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https://www.readbyqxmd.com/read/27917926/clonal-spread-of-mcr-1-in-pmqr-carrying-st34-salmonella-isolates-from-animals-in-china
#1
Xing-Ping Li, Liang-Xing Fang, Jia-Qi Song, Jing Xia, Wei Huo, Jin-Tao Fang, Xiao-Ping Liao, Ya-Hong Liu, Youjun Feng, Jian Sun
Since initial identification in China, the widespread geographical occurrence of plasmid-mediated colistin resistance gene mcr-1 in Enterobacteriaceae has been of great concern. In this study, a total of 22 Salmonella enterica were resistant to colistin, while only five isolates which belonged to ST34 Salmonella enterica serovar Typhimurium (S. Typhimurium) were mcr-1 positive. Four of them shared nearly identical PFGE type, although they were from different host species and diverse geographical locations. All the mcr-1-positive S...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27767038/isolation-and-plasmid-characterization-of-carbapenemase-imp-4-producing-salmonella-enterica-typhimurium-from-cats
#2
Sam Abraham, Mark O'Dea, Darren J Trott, Rebecca J Abraham, David Hughes, Stanley Pang, Genevieve McKew, Elaine Y L Cheong, John Merlino, Sugiyono Saputra, Richard Malik, Thomas Gottlieb
Carbapenem-resistant Enterobacteriaceae (CRE) are a pressing public health issue due to limited therapeutic options to treat such infections. CREs have been predominantly isolated from humans and environmental samples and they are rarely reported among companion animals. In this study we report on the isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from a companion animal. Carbapenemase-producing S. enterica Typhimurium carrying blaIMP-4 was identified from a systemically unwell (index) cat and three additional cats at an animal shelter...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27746775/inchi2-plasmids-are-predominant-in-antibiotic-resistant-salmonella-isolates
#3
Wenyao Chen, Tingzi Fang, Xiujuan Zhou, Daofeng Zhang, Xianming Shi, Chunlei Shi
The wide usage of antibiotics contributes to the increase in the prevalence of antibiotic-resistant Salmonella. Plasmids play a critical role in horizontal transfer of antibiotic resistance markers in Salmonella. This study aimed to screen and characterize plasmid profiles responsible for antibiotic resistance in Salmonella and ultimately to clarify the molecular mechanism of transferable plasmid-mediated antibiotic resistance. A total of 226 Salmonella isolates were examined for antimicrobial susceptibility by a disk diffusion method...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27572409/inchi2-plasmids-are-the-key-vectors-responsible-for-oqxab-transmission-among-salmonella-spp
#4
Marcus Ho-Yin Wong, Edward Wai-Chi Chan, Liqi Xie, Ruichao Li, Sheng Chen
This study reported and analysed the complete sequences of two oqxAB-bearing IncHI2 plasmids harboured by a clinical S. Typhimurium strain and an S. Indiana strain of animal origin, respectively. Particularly, pA3T recovered from S. Indiana comprised the resistance determinants oqxAB, aac(6')Ib-cr, fosA3 and blaCTX-M-14 Further genetic screening of 63 oqxAB-positive Salmonella spp. isolates revealed that the majority carried IncHI2 plasmids, confirming that such plasmids play a pivotal role in dissemination of oqxAB in Salmonella spp...
August 29, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27572407/genome-and-plasmid-analysis-of-blaimp-4-carrying-citrobacter-freundii-b38
#5
Jianhui Xiong, Maxime Déraspe, Naeem Iqbal, Jennifer Ma, Frances B Jamieson, Jessica Wasserscheid, Ken Dewar, Peter M Hawkey, Paul H Roy
Sequencing of the blaIMP-4 -carrying C. freundii B38 using PacBio SMRT technique revealed that the genome contained a chromosome of 5,134,500 bp, and three plasmids, pOZ172 (127,005 bp), pOZ181 (277,592 bp), and pOZ182 (18,467 bp). Plasmid pOZ172 was identified as IncFIIY, like pP10164-NDM and pNDM-EcGN174. It carries a class 1 integron with four cassettes: blaIMP-4-qacG2-aacA4-aphA15, and a complete hybrid tni module (tniR-tniQ-tniB-tniA). The recombination of tniR from Tn402 (identical) with tniQBA (99%) from Tn5053 occurred within the res site of Tn402/5053...
August 29, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27572405/characterization-of-a-novel-inchi2-plasmid-carrying-tandem-copies-of-blactx-m-2-in-a-fosa6-harboring-escherichia-coli-st410-strain
#6
Qinglan Guo, Baixing Ding, Thomas Jové, Nicole Stoesser, Vaughn S Cooper, Minggui Wang, Yohei Doi
Extended-spectrum β-lactamase gene blaCTX-M-2 is mainly associated with ISCR1 embedded in complex sul1-type integrons, but information on the genetic context of plasmids harboring the ISCR1-blaCTX-M-2module remains limited. In this study, a blaCTX-M-2-harboring plasmid pYD786-1 belonging to novel ST2-IncHI2 was sequenced and analyzed from an Escherichia coli ST410 clinical strain. pYD786-1 belonged to APEC-O1-R type IncHI2 plasmids which are widely distributed in human, poultry and livestock strains. It contained a multidrug resistance mosaic region (MRR) consisting of Tn21::In2 transposon backbone augmented by acquisition of duplicated ISCR1-blaCTX-M-2 Tn2411, a Tn21::In2 precursor, likely played a role in the generation of the MRR in pN13-01290_23, the putative progenitor plasmid of pYD786-1 found in a foodborne Salmonella strain...
August 29, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27566913/plasmid-mediated-colistin-resistance-in-escherichia-coli-from-the-arabian-peninsula
#7
Ágnes Sonnevend, Akela Ghazawi, Manaf Alqahtani, Atef Shibl, Wafa Jamal, Rayhan Hashmey, Tibor Pal
OBJECTIVES: Searching for the presence of the mcr-1 gene in colistin resistant Enterobacteriaceae in countries of the Arabian Peninsula. METHODS: Seventy-five independent, colistin resistant Enterobacteriaceae strains isolated from clinical cases in Bahrain, Kuwait, Oman, Saudi Arabia and the United Arab Emirates were tested by PCR for the mcr-1 gene. mcr-1 positive strains were genotyped, and their antibiotic susceptibility was established. The mcr-1 containing plasmids were mobilized into Escherichia coli K-12 and their sequence was determined...
September 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27550364/emergence-of-ndm-5-and-mcr-1-producing-escherichia-coli-clone-st648-and-st156-from-a-single-muscovy-duck-cairina-moschata
#8
Run-Shi Yang, Youjun Feng, Xiao-Yue Lv, Jia-Hong Duan, Jing Chen, Liang-Xing Fang, Jing Xia, Xiao-Ping Liao, Jian Sun, Ya-Hong Liu
Two E. coli clones (ST648 and ST156) that coproduce NDM-5 and MCR-1 were detected from a single fowl in China. The blaNDM-5 gene was found on the two indistinguishable IncX3 plasmids from the two different E. coli isolates, whereas the mcr-1 gene was located on IncHI2 and IncI2 plasmid respectively, suggesting that blaNDM-5 andmcr-1 have spread in avian intestinal flora. Also, the two strains harbor blaTEM-1, blaCTX-M-55, fosA3 and aac(6')-Ib The multi-resistant E. coli (esp. the epidemic clone ST648) might raise a potential threat to human health via food-chain transmission...
August 22, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27436392/spread-of-enterobacter-cloacae-carrying-blandm-1-blactx-m-15-blashv-12-and-plasmid-mediated-quinolone-resistance-genes-in-a-surgical-intensive-care-unit-in-croatia
#9
N Petrosillo, M Vranić-Ladavac, C Feudi, L Villa, D Fortini, N Barišić, B Bedenić, R Ladavac, S D'Arezzo, A Tambić Andrašević, A Capone
The objective of this study was to describe a hospital cluster of NDM-1-producing Enterobacter cloacae infections observed in the surgical intensive care unit (ICU) of a tertiary-care hospital at Pula, Croatia. NDM-1-producing E. cloacae strains isolated from clinical samples were screened by PCR for the presence of carbapenemases. Genetic relatedness of NDM-1-producing E. cloacae strains was determined by multilocus sequence typing (MLST). During the period October 2013 to April 2014, four patients, with overlapping hospital stay in the surgical ICU, developed severe infections caused by E...
March 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27387036/mcr-1-in-multidrug-resistant-and-copper-tolerant-clinically-relevant-salmonella-1-4-5-12-i-and-s-rissen-clones-in-portugal-2011-to-2015
#10
Joana Campos, Luís Cristino, Luísa Peixe, Patrícia Antunes
The mcr-1 gene was found in 11 isolates from a Portuguese Salmonella collection (n = 1,010; 58 serotypes; 2002-15) of clinical samples, foodstuff, food-animals and water. Mcr-1 has been located on different plasmids (IncX4/IncHI2) in pig-associated multidrug-resistant, copper-tolerant S.1,4,[5],12:i:-/ST34 and S. Rissen/ST469 clones from human and pork products since at least 2011. Our data highlight dissemination of mcr-1 by successful resistant clones in Europe and raise questions about the efficacy of copper-based interventions to reduce colistin use...
June 30, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/27324774/full-length-nucleotide-sequences-of-mcr-1-harboring-plasmids-isolated-from-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolates-of-different-origins
#11
Katrin Zurfluh, Jochen Klumpp, Magdalena Nüesch-Inderbinen, Roger Stephan
Here, we present the full sequences of three mcr-1-carrying plasmids isolated from extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli The plasmids belong to three different replicon types and are 34,640 bp, 209,401 bp, and 247,885 bp in size. We describe for the first time a composite transposon containing mcr-1 localized on a multidrug-resistant (MDR) IncHI2 plasmid harboring additional determinants of resistance to six different classes of antibiotics, including the ESBL gene blaCTX-M-1, and heavy metal resistance...
September 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27318267/pdgo100-a-type-1-incc-plasmid-from-1981-carrying-ari-a-and-a-tn1696-like-transposon-in-a-novel-integrating-element
#12
Christopher J Harmer, Sally R Partridge, Ruth M Hall
Most A/C plasmids sequenced to date were recovered in the last two decades. To gain insight into the evolution of this group, the IncC plasmid pDGO100, found in a multiply antibiotic-resistant Escherichia coli strain isolated in 1981, was sequenced. pDGO100 belongs to the type 1 lineage and carries an ARI-A antibiotic resistance island but not an ARI-B island. The A/C2 backbone of pDGO100 has a deletion in the rhs1 gene previously found in pRMH760 and differs by only six single base pair substitutions from pRMH760, recovered at the same hospital 16years later...
July 2016: Plasmid
https://www.readbyqxmd.com/read/27302904/diversity-of-class-1-and-2-integrons-detected-in-escherichia-coli-isolates-from-diseased-and-apparently-healthy-dogs
#13
Amanda K Siqueira, Geovana B Michael, Daniela F Domingos, Mirtis M G Ferraz, Márcio G Ribeiro, Stefan Schwarz, Domingos S Leite
Escherichia coli is one of the major pathogens causing urinary tract infections (UTIs) and pyometra in dogs. The aims of this study were to investigate canine E. coli isolates for the presence of class 1 and 2 integrons by PCR/sequencing and to characterize these isolates and their integron-carrying plasmids. Isolates were characterized by phylotyping, XbaI-macrorestriction analysis and plasmid transfer experiments. Plasmids were analyzed by S1 nuclease-PFGE, replicon typing, conjugation and restriction analysis...
May 13, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27297483/travelers-can-import-colistin-resistant-enterobacteriaceae-including-those-possessing-the-plasmid-mediated-mcr-1-gene
#14
Odette J Bernasconi, Esther Kuenzli, João Pires, Regula Tinguely, Alessandra Carattoli, Christoph Hatz, Vincent Perreten, Andrea Endimiani
Stool samples from 38 travelers returning from India were screened for extended-spectrum cephalosporin- and carbapenem-resistant Enterobacteriaceae implementing standard selective plates. Twenty-six (76.3%) people were colonized with CTX-M or DHA producers, but none of the strains was colistin resistant and/or mcr-1 positive. Nevertheless, using overnight enrichment and CHROMagar Orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistant Escherichia coli One cephalosporin-susceptible sequence type 10 (ST10) strain carried a 4,211-bp ISApl1-mcr-1-ISApl1 element in an IncHI2 plasmid backbone...
August 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27143648/co-spread-of-metal-and-antibiotic-resistance-within-st3-inchi2-plasmids-from-e-coli-isolates-of-food-producing-animals
#15
Liangxing Fang, Xingping Li, Liang Li, Shumin Li, Xiaoping Liao, Jian Sun, Yahong Liu
Concerns have been raised in recent years regarding co-selection for antibiotic resistance among bacteria exposed to heavy metals, particularly copper and zinc, used as growth promoters for some livestock species. In this study, 25 IncHI2 plasmids harboring oqxAB (20/25)/blaCTX-M (18/25) were found with sizes ranging from ∼260 to ∼350 kb and 22 belonged to the ST3-IncHI2 group. In addition to blaCTX-M and oqxAB, pcoA-E (5/25) and silE-P (5/25), as well as aac(6')-Ib-cr (18/25), floR (16/25), rmtB (6/25), qnrS1(3/25) and fosA3 (2/25), were also identified on these IncHI2 plasmids...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27118781/tolerance-to-multiple-metal-stressors-in-emerging-non-typhoidal-mdr-salmonella-serotypes-a-relevant-role-for-copper-in-anaerobic-conditions
#16
Joana Mourão, Sara Marçal, Paula Ramos, Joana Campos, Jorge Machado, Luísa Peixe, Carla Novais, Patrícia Antunes
OBJECTIVES: Factors driving the expansion of particular MDR Salmonella serotypes/clones are not completely understood. We assessed if emergent MDR Salmonella serotypes/clones were more enriched in metal tolerance genes (e.g. to Cu/Ag) than other less frequent ones, as an additional feature to survive in environments contaminated with metals. METHODS: Metal (Cu pco/Ag,Cu sil/Hg mer/As ars/Te ter) tolerance genes screening (PCR/sequencing), MICs of CuSO4/AgNO3 (aerobiosis/anaerobiosis), genetic element characterization (S1/I-CeuI PFGE) and conjugation assays were performed in a well-characterized Salmonella collection (n = 275 isolates; 2000-14; 49 serotypes/clones)...
August 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27101788/complete-nucleotide-sequence-of-ph11-an-inchi2-plasmid-conferring-multi-antibiotic-resistance-and-multi-heavy-metal-resistance-genes-in-a-clinical-klebsiella-pneumoniae-isolate
#17
Yao Zhai, Zilong He, Yu Kang, Haiying Yu, Jianfeng Wang, Pengcheng Du, Zhao Zhang, Songnian Hu, Zhancheng Gao
The complete 284,628bp sequence of pH11, an IncHI2 plasmid, was determined through single-molecule, real-time (SMRT) sequencing. Harbored by a clinical Klebsiella pneumoniae strain H11, and isolated in Beijing, this plasmid contains multiple antibiotic resistance genes, including catA2, aac(6')-Ib, strB, strA, dfrA19, blaTEM-1, blaSHV-12, sul1, qacE delta 1, ereA, arr2, and aac3. The aac(6')-Ib is carried by a class I integron. Plasmid pH11 also carries several genes associated with resistance to heavy metals, such as tellurium, mercury, cobalt, zinc, nickel, copper, lead and cadmium...
July 2016: Plasmid
https://www.readbyqxmd.com/read/27090630/detection-of-the-plasmid-mediated-mcr-1-gene-conferring-colistin-resistance-in-human-and-food-isolates-of-salmonella-enterica-and-escherichia-coli-in-england-and-wales
#18
Michel Doumith, Gauri Godbole, Philip Ashton, Lesley Larkin, Tim Dallman, Martin Day, Michaela Day, Berit Muller-Pebody, Matthew J Ellington, Elizabeth de Pinna, Alan P Johnson, Katie L Hopkins, Neil Woodford
OBJECTIVES: In response to the first report of transmissible colistin resistance mediated by the mcr-1 gene in Escherichia coli and Klebsiella spp. from animals and humans in China, we sought to determine its presence in Enterobacteriaceae isolated in the UK. METHODS: The PHE archive of whole-genome sequences of isolates from surveillance collections, submissions to reference services and research projects was retrospectively analysed for the presence of mcr-1 using Genefinder...
August 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27065989/ctx-m-27-producing-salmonella-enterica-serotypes-typhimurium-and-indiana-are-prevalent-among-food-producing-animals-in-china
#19
Wen-Hui Zhang, Xiang-Yan Lin, Liang Xu, Xi-Xi Gu, Ling Yang, Wan Li, Si-Qi Ren, Ya-Hong Liu, Zhen-Ling Zeng, Hong-Xia Jiang
Salmonella spp. is one of the most important food-borne pathogens causing digestive tract and invasive infections in both humans and animals. Extended-spectrum β-lactamases (ESBLs) especially the CTX-M-type ESBLs are increasingly being reported worldwide and in China. These studies seldom focused on Salmonella isolates from food-producing animals. The aim of this study was to characterize the antimicrobial resistance profiles, serotypes and ESBLs and in particular, CTX-M producing Salmonella isolates from chickens and pigs in China...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26940999/impact-of-food-animal-trade-on-the-spread-of-mcr-1-mediated-colistin-resistance-tunisia-july-2015
#20
Raoudha Grami, Wejdene Mansour, Wahib Mehri, Olfa Bouallègue, Noureddine Boujaâfar, Jean-Yves Madec, Marisa Haenni
We report a high prevalence of MCR-1 and CTX-M-1-producing Escherichia coli in three Tunisian chicken farms. Chickens were imported from France or derived from French imported chicks. The same IncHI2-type plasmid reported to carry those genes in cattle in France and in a food sample in Portugal was found in Tunisian chickens of French origin. This suggests a significant impact of food animal trade on the spread of mcr-1-mediated colistin resistance in Europe.
2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
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