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https://www.readbyqxmd.com/read/29180279/impact-of-the-colistin-resistance-gene-mcr-1-on-bacterial-fitness
#1
Manuela Tietgen, Torsten Semmler, Sara Riedel-Christ, Volkhard A J Kempf, Antonio Molinaro, Christa Ewers, Stephan Göttig
A Klebsiella pneumoniae isolate harbouring a 217 kb IncHI2-type plasmid, termed pKP2442, encoding the colistin resistance gene mcr-1 was isolated from a leukaemia patient. pKP2442 was mobilized by intra- and intergenus transconjugation from the clinical isolate to E. coli J53 (transconjugation frequency: 6.86 x 10-8 ± 5.57 x 10-8) and K. pneumoniae PRZ (4.04 x 10-8 ± 3.03 x 10-8), respectively. Since acquisition of resistance determinants often results in a loss of fitness, the impact of mcr-1 on fitness of E...
November 24, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29148380/high-rate-of-mcr-1-producing-escherichia-coli-and-klebsiella-pneumoniae-among-pigs-portugal
#2
Nicolas Kieffer, Marta Aires-de-Sousa, Patrice Nordmann, Laurent Poirel
The mcr-1 (mobile colistin resistance 1) gene, which encodes phosphoethanolamine transferase, has been recently identified as a source of acquired resistance to polymyxins in Escherichia coli. Using the SuperPolymyxin selective medium, we prospectively screened 100 pigs at 2 farms in Portugal for polymyxin-resistant Enterobacteriaceae and recovered 98 plasmid-mediated MCR-1-producing isolates. Most isolates corresponded to nonclonally related E. coli belonging to many sequence types; we also found 2 Klebsiella pneumoniae sequence types...
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29127343/global-phylogenetic-analysis-of-escherichia-coli-and-plasmids-carrying-the-mcr-1-gene-indicates-bacterial-diversity-but-plasmid-restriction
#3
Sébastien Matamoros, Jarne M van Hattem, Maris S Arcilla, Niels Willemse, Damian C Melles, John Penders, Trung Nguyen Vinh, Ngo Thi Hoa, Menno D de Jong, Constance Schultsz
To understand the dynamics behind the worldwide spread of the mcr-1 gene, we determined the population structure of Escherichia coli and of mobile genetic elements (MGEs) carrying the mcr-1 gene. After a systematic review of the literature we included 65 E. coli whole genome sequences (WGS), adding 6 recently sequenced travel related isolates, and 312 MLST profiles. We included 219 MGEs described in 7 Enterobacteriaceae species isolated from human, animal and environmental samples. Despite a high overall diversity, 2 lineages were observed in the E...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29118748/remarkable-diversity-of-escherichia-coli-carrying-mcr-1-from-hospital-sewage-with-the-identification-of-two-new-mcr-1-variants
#4
Feifei Zhao, Yu Feng, Xiaoju Lü, Alan McNally, Zhiyong Zong
The plasmid-borne colistin-resistant gene mcr-1 has rapidly become a worldwide public health concern. This study aims to determine the host bacterial strains, plasmids, and genetic contexts of mcr-1 in hospital sewage. A 1-ml hospital sewage sample was cultured. Colistin-resistant bacterial colonies were selected on agar plates and were subjected to whole genome sequencing and subsequent analysis. The transfer of mcr-1 between bacterial strains was tested using conjugation. New variants of mcr-1 were cloned to test the impact of variations on the function of mcr-1...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29103683/complete-genetic-analysis-of-a-salmonella-enterica-serovar-indiana-isolate-accompanying-four-plasmids-carrying-mcr-1-esbl-and-other-resistance-genes-in-china
#5
Juan Wang, Xianglei Li, Juan Li, Daniel Hurley, Xue Bai, Zhongyi Yu, Yu Cao, Ellen Wall, Séamus Fanning, Li Bai
One mcr-1-carrying Salmonella enterica serovar Indiana strain D90, was identified from 1320 Salmonella enterica isolates from poultry slaughterhouse in 2012 in China. The objective of this study was to verify the transferability of the mcr-1 gene and also completely characterize the sequence of the strain at the whole-genome level. Broth matting assays were carried out to detect the transferability and whole-genome sequencing (WGS) of S. enterica serovar Indiana D90 was performed using the PacBio RS II system...
October 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29091201/is26-mediated-formation-of-a-virulence-and-resistance-plasmid-in-salmonella-enteritidis
#6
Marcus Ho-Yin Wong, Edward Wai-Chi Chan, Sheng Chen
Objectives: To characterize a novel virulence-resistance plasmid pSE380T carried by a Salmonella enterica serotype Enteritidis clinical strain SE380. Methods: The plasmid pSE380T was conjugated to Escherichia coli strain J53 and sequenced by PacBio RSII, followed by subsequent annotation and genetic analysis. Results: Sequence analysis of this plasmid revealed that the entire Salmonella Enteritidis-specific virulence plasmid, pSEN, had been incorporated into an IncHI2 MDR plasmid, which comprises the cephalosporin and fosfomycin resistance determinants blaCTX-M-14 and fosA3...
October 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29081769/characterization-of-oqxab-in-escherichia-coli-isolates-from-animals-retail-meat-and-human-patients-in-guangzhou-china
#7
Jing Wang, Chan-Ping Zhi, Xiao-Jie Chen, Ze-Wen Guo, Wu-Ling Liu, Juan Luo, Xin-Yi Huang, Li Zeng, Jia-Wei Huang, Ying-Bi Xia, Meng-Ying Yi, Teng Huang, Zhen-Ling Zeng, Jian-Hua Liu
The purpose of this study was to investigate the prevalence and genetic elements of oqxAB among Escherichia coli isolates from animals, retail meat, and humans (patients with infection or colonization) in Guangzhou, China. A total of 1,354 E. coli isolates were screened for oqxAB by PCR. Fifty oqxAB-positive isolates were further characterized by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), S1-PFGE, genetic environment analysis, plasmid replicon typing, and plasmid sequencing...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29061750/dissemination-and-characteristics-of-a-novel-plasmid-encoded-carbapenem-hydrolyzing-class-d-%C3%AE-lactamase-oxa-436-from-four-patients-involving-six-different-hospitals-in-denmark
#8
Ørjan Samuelsen, Frank Hansen, Bettina Aasnæs, Henrik Hasman, Bjarte Aarmo Lund, Hanna-Kirsti S Leiros, Berit Lilje, Jessin Janice, Lotte Jakobsen, Pia Littauer, Lillian M Søes, Barbara J Holzknecht, Leif P Andersen, Marc Stegger, Paal S Andersen, Anette M Hammerum
The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D carbapenemase (CHDL) named OXA-436. In total, six OXA-436-producing Enterobacteriaceae isolates including Enterobacter asburiae (n=3), Citrobacter freundii (n=2) and Klebsiella pneumoniae (n=1) were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing Enterobacteriaceae were found in one patient...
October 23, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29051246/complete-genome-sequence-of-a-colistin-resistant-escherichia-coli-strain-harboring-mcr-1-on-an-inchi2-plasmid-in-the-united-states
#9
Victoria L Gilrane, Stephen Lobo, Weihua Huang, Jian Zhuge, Changhong Yin, Donald Chen, Kimberly J Alvarez, Alexandra Budhai, Ilana Nadelman, Nevenka Dimitrova, John T Fallon, Guiqing Wang
We report here the incidental detection and complete genome sequence of a urinary Escherichia coli strain harboring mcr-1 and resistant to colistin in a New York patient returning from Portugal in 2016. This strain, with sequence type 1485 (ST1485), was a non-extended-spectrum beta-lactamase (ESBL) and non-carbapenemase producer and carried the mcr-1 gene on an IncHI2 plasmid.
October 19, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29040419/high-rates-of-human-fecal-carriage-of-mcr-1-positive-multi-drug-resistant-enterobacteriaceae-isolates-emerge-in-china-in-association-with-successful-plasmid-families
#10
Lan-Lan Zhong, Hang T T Phan, Cong Shen, Karina Doris-Vihta, Anna E Sheppard, Xi Huang, Kun-Jiao Zeng, Hong-Yu Li, Xue-Fei Zhang, Sandip Patil, Derrick W Crook, A Sarah Walker, Yong Xing, Jia-Lin Lin, Lian-Qiang Feng, Yohei Doi, Yong Xia, Nicole Stoesser, Guo-Bao Tian
Objectives: mcr-1-mediated colistin resistance in Enterobacteriaceae is concerning, as colistin is used in treating multidrug-resistant Enterobacteriaceae infections. Rates of human mcr-1 gastrointestinal carriage have historically been low. We identified trends in human fecal mcr-1-positivity rates and colonization with mcr-1-positive+third-generation cephalosporin-resistant (3GC-R) Enterobacteriaceae in Guangzhou, China, and investigated the genetic contexts of mcr-1 in a subset of mcr-1-positive+3GC-R strains...
October 10, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29038441/distinct-mechanisms-of-acquisition-of-mcr-1-bearing-plasmid-by-salmonella-strains-recovered-from-animals-and-food-samples
#11
Mingquan Cui, Jinfei Zhang, Chunping Zhang, Ruichao Li, Edward Wai-Chi Chan, Chenbin Wu, Congming Wu, Sheng Chen
Since the report of its discovery in E. coli in late 2015, the plasmid-mediated colistin resistance gene, mcr-1, has been detected in various bacterial species in clinical setting and various environmental niches. However, the transmission mechanisms of this gene in Salmonella is less defined. In this study, we conducted a comprehensive study to characterize the genetic features of mcr-1-positive Salmonella strains isolated from animals and foods. Our data revealed that Salmonella recovered from animals and food specimens exhibited highly different PFGE patterns, and acquired mcr-1-encoding plasmids via different mechanism...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28830739/molecular-bases-for-multidrug-resistance-in-yersinia-pseudotuberculosis
#12
Nicolas Cabanel, Marc Galimand, Christiane Bouchier, Margarita Chesnokova, Valery Klimov, Elisabeth Carniel
The enteropathogen Yersinia pseudotuberculosis causes gastrointestinal infections in humans. Although this species is usually susceptible to antibiotics active against Gram-negative bacteria, we identified three multidrug resistant (MDR) strains of Y. pseudotuberculosis that were isolated from the environment in Russia and from a patient in France. The resistance traits of the two Russian isolates were transferable at high frequencies (≈2×10(-1)/donor CFU) to Y. pseudotuberculosis. In contrast no transfer of the antibiotic resistances carried by the French strain was observed...
August 16, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28818900/genome-sequence-of-an-australian-monophasic-salmonella-enterica-subsp-enterica-typhimurium-isolate-tw-stm6-carrying-a-large-plasmid-with-multiple-antimicrobial-resistance-genes
#13
Mike L Dyall-Smith, Yuhong Liu, Helen Billman-Jacobe
We report the genome sequence of a monophasic Salmonella enterica subsp. enterica Typhimurium strain (TW-Stm6) isolated in Australia that is similar to epidemic multidrug-resistant strains from Europe and elsewhere. This strain carries additional antibiotic and heavy-metal resistance genes on a large (275-kb) IncHI2 plasmid.
August 17, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28797324/novel-mcr-3-variant-encoding-mobile-colistin-resistance-in-an-st131-escherichia-coli-isolate-from-bloodstream-infection-denmark-2014
#14
Louise Roer, Frank Hansen, Marc Stegger, Ute Wolff Sönksen, Henrik Hasman, Anette M Hammerum
A novel variant of the plasmid-borne colistin resistance gene mcr-3 was detected on an IncHI2 plasmid in an ST131 CTX-M-55-producing Escherichia coli isolate from a Danish patient with bloodstream infection in 2014. The discovery of novel plasmid-borne genes conferring resistance to colistin is of special interest since colistin has reemerged as an important drug in the treatment of infections with multidrug-resistant Gram-negative bacteria.
August 3, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28771073/co-occurrence-of-3-different-resistance-plasmids-in-a-multi-drug-resistant-cronobacter-sakazakii-isolate-causing-neonatal-infections
#15
Lining Shi, Quanhui Liang, Zhe Zhan, Jiao Feng, Yachao Zhao, Yong Chen, Mei Huang, Yigang Tong, Weili Wu, Weijun Chen, Xiaojun Li, Zhe Yin, Jinglin Wang, Dongsheng Zhou
Cronobacter sakazakii 505108 was isolated from a sputum specimen of a neonate with severe pneumonia. C. sakazakii 505108 co-harbors 3 resistance plasmids of the IncHI2, IncX3, and IncFIB incomparability groups, respectively. These 3 plasmids have acquired several accessory modules, which carry an extremely large number of resistance genes, especially including those involved in resistance to carbapenems, aminoglycoside, tetracyclines, and phenicols and sulphonamide/trimethoprim. These plasmid-borne antibiotic resistance genes were associated with insertion sequences, integrons, and transposons, indicating that the assembly and mobilization of the corresponding accessory modules with complex chimera structures are facilitated by transposition and/or homologous recombination...
August 3, 2017: Virulence
https://www.readbyqxmd.com/read/28757017/characterization-of-ndm-5-positive-extensively-resistant-escherichia-coli-isolates-from-dairy-cows
#16
Tao He, Ruicheng Wei, Lili Zhang, Lichang Sun, Maoda Pang, Ran Wang, Yang Wang
The aim of this study was to investigate the prevalence of blaNDM-5 gene in Escherichia coli isolates from dairy cows and to characterize the molecular traits of the blaNDM-5-positive isolates. A total of 169 cows were sampled (169 feces and 169 raw milk samples) in three dairy farms in Jiangsu Province and 203 E. coli isolates were recovered. Among these strains, three isolates carried blaNDM-5 gene, including one co-harboring mcr-1, which belonged to sequence type 446 and the other two belonged to ST2. Susceptibility testing revealed that the three blaNDM-5-positive isolates showed extensive resistance to antimicrobials...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28739785/emergence-of-mcr-1-in-raoultella-ornithinolytica-and-escherichia-coli-isolates-from-retail-vegetables-in-china
#17
Juan Luo, Xu Yao, Luchao Lv, Yohei Doi, Xiuyu Huang, Sicheng Huang, Jian-Hua Liu
The presence of mcr-1 among Enterobacteriaceae isolates collected from retail vegetables in China between 2015 and 2016 was investigated. Two Raoultella ornithinolytica and seven Escherichia coli strains recovered from lettuce and tomato samples were identified as MCR-1-producers. Similar to isolates from animals and humans, the mcr-1 gene was located on the IncHI2/ST3, IncI2, or IncX4 plasmids. The presence of MCR-1-producing organisms in ready-to-eat food samples represents a serious risk for human health...
October 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28655818/novel-plasmid-mediated-colistin-resistance-gene-mcr-3-in-escherichia-coli
#18
Wenjuan Yin, Hui Li, Yingbo Shen, Zhihai Liu, Shaolin Wang, Zhangqi Shen, Rong Zhang, Timothy R Walsh, Jianzhong Shen, Yang Wang
The mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant Gram-negative bacteria. To date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the GenBank database. Here, we characterized a third mobile colistin resistance gene, mcr-3 The gene coexisted with 18 additional resistance determinants in the 261-kb IncHI2-type plasmid pWJ1 from porcine Escherichia colimcr-3 showed 45...
June 27, 2017: MBio
https://www.readbyqxmd.com/read/28643490/characterization-of-extended-spectrum-%C3%AE-lactamase-genes-of-shigella-flexneri-isolates-with-fosfomycin-resistance-from-patients-in-china
#19
Yanyan Liu, Yue Cheng, Haifei Yang, Lifen Hu, Jun Cheng, Ying Ye, Jiabin Li
BACKGROUND: The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flexneri from patients in China. METHODS: A total of 263 S. flexneri isolates were collected from 34 hospitals in the Anhui Province of China during September 2012-September 2015 and screened for fosA3, fosA, and fosC2 by PCR amplification and sequencing...
September 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28642846/decreased-fitness-and-virulence-in-st10-escherichia-coli-harboring-blandm-5-and-mcr-1-against-a-st4981-strain-with-blandm-5
#20
Yawei Zhang, Kang Liao, Hua Gao, Qi Wang, Xiaojuan Wang, Henan Li, Ruobing Wang, Hui Wang
Although coexistence of blaNDM-5 and mcr-1 in Escherichia coli has been reported, little is known about the fitness and virulence of such strains. Three carbapenem-resistant Escherichia coli (GZ1, GZ2, and GZ3) successively isolated from one patient in 2015 were investigated for microbiological fitness and virulence. GZ1 and GZ2 were also resistant to colistin. To verify the association between plasmids and fitness, growth kinetics of the transconjugants were performed. We also analyzed genomic sequences of GZ2 and GZ3 using PacBio sequencing...
2017: Frontiers in Cellular and Infection Microbiology
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