keyword
MENU ▼
Read by QxMD icon Read
search

Stem cell replacement

keyword
https://www.readbyqxmd.com/read/28734258/mechanical-properties-and-biocompatibility-of-porous-titanium-scaffolds-for-bone-tissue-engineering
#1
Yunhui Chen, Jessica Ellen Frith, Ali Dehghan-Manshadi, Hooyar Attar, Damon Kent, Nicolas Dominique Mathieu Soro, Michael J Bermingham, Matthew S Dargusch
Synthetic scaffolds are a highly promising new approach to replace both autografts and allografts to repair and remodel damaged bone tissue. Biocompatible porous titanium scaffold was manufactured through a powder metallurgy approach. Magnesium powder was used as space holder material which was compacted with titanium powder and removed during sintering. Evaluation of the porosity and mechanical properties showed a high level of compatibility with human cortical bone. Interconnectivity between pores is higher than 95% for porosity as low as 30%...
July 13, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28732241/generation-of-skeletal-myogenic-progenitors-from-human-pluripotent-stem-cells-using-non-viral-delivery-of-minicircle-dna
#2
Jaemin Kim, Vanessa K P Oliveira, Ami Yamamoto, Rita C R Perlingeiro
Currently, the most efficient and promising approach for generating large numbers of engraftable human skeletal myogenic progenitors from pluripotent stem cells requires the conditional in vitro overexpression of PAX7 using lentiviral vectors. Because a non-integrating approach would be preferable to eliminate or minimize the risk associated with random genomic integration, here we investigate whether transient expression of PAX7 using minicircle DNA would enable the generation of functional pluripotent stem cell-derived myogenic progenitors, equivalent to those generated by lentivirus...
July 12, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28730879/rgrna-prediction-of-rna-secondary-structure-based-on-replacement-and-growth-of-stems
#3
Jin Li, Chengzhen Xu, Hong Liang, Wang Cong, Ying Wang, Kuan Luan, Yunlong Liu
Owing to their structural diversity, RNAs perform many diverse biological functions in the cell. RNA secondary structure is thus important for predicting RNA function. Here, we propose a new combinatorial optimization algorithm, named RGRNA, to improve the accuracy of predicting RNA secondary structure. Following the establishment of a stempool, the stems are sorted by length, and chosen from largest to smallest. If the stem selected is the true stem, the secondary structure of this stem when combined with another stem selected at random will have low free energy, and the free energy will tend to gradually diminish...
July 21, 2017: Computer Methods in Biomechanics and Biomedical Engineering
https://www.readbyqxmd.com/read/28730556/stem-cell-derived-retinal-pigment-epithelium-the-role-of-pigmentation-as-maturation-marker-and-gene-expression-profile-comparison-with-human-endogenous-retinal-pigment-epithelium
#4
A Bennis, J G Jacobs, L A E Catsburg, J B Ten Brink, C Koster, R O Schlingemann, J van Meurs, T G M F Gorgels, P D Moerland, V M Heine, A A Bergen
In age-related macular degeneration (AMD) the retinal pigment epithelium (RPE) deteriorates, leading to photoreceptor decay and severe vision loss. New therapeutic strategies aim at RPE replacement by transplantation of pluripotent stem cell (PSC)-derived RPE. Several protocols to generate RPE have been developed where appearance of pigmentation is commonly used as indicator of RPE differentiation and maturation. It is, however, unclear how different pigmentation stages reflect developmental stages and functionality of PSC-derived RPE cells...
July 21, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28730239/wnt-and-nodal-signaling-simultaneously-induces-definitive-endoderm-differentiation-of-mouse-embryonic-stem-cells
#5
Wa Zhong, Yu Lai, Tao Yu, Zhong Sheng Xia, Yu Hong Yuan, Hui Ouyang, Ti Dong Shan, Qi Kui Chen
Induced differentiation of definitive endoderm (DE) from embryonic stem cells (ESCs) has been the recent focus of studies investigating regeneration and transplantation of organs of the digestive system. Poor cell survival is the most important challenge to DE differentiation from ESCs. This study aimed to optimize culture conditions to promote the differentiation of mouse ESCs into DE, and to investigate the roles of the Wnt and Nodal signaling pathways in the DE differentiation. The mouse ESCs were treated with or without leukemia inhibitory factor, Wnt3a and Activin A alone or together, and examined the DE differentiation by the DE marker CXCR4 and the ESC marker Oct4...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28729666/expansion-of-human-midbrain-floor-plate-progenitors-from-induced-pluripotent-stem-cells-increases-dopaminergic-neuron-differentiation-potential
#6
Stefania Fedele, Ginetta Collo, Katharina Behr, Josef Bischofberger, Stephan Müller, Tilo Kunath, Klaus Christensen, Anna Lisa Gündner, Martin Graf, Ravi Jagasia, Verdon Taylor
Human induced pluripotent stem cells (hiPSCs) are invaluable to study developmental processes and disease mechanisms particularly in the brain. hiPSCs can be differentiated into mature and functional dopaminergic (DA) neurons. Having robust protocols for the generation of differentiated DA neurons from pluripotent cells is a prerequisite for the use of hiPSCs to study disease mechanisms, for drug discovery, and eventually for cell replacement therapy. Here, we describe a protocol for generating and expanding large numbers of homogeneous midbrain floor plate progenitors (mFPPs) that retain efficient DA neurogenic potential over multiple passages and can be cryobanked...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729539/comparative-proteomic-analysis-of-human-embryonic-stem-cell-derived-and-primary-human-retinal-pigment-epithelium
#7
Heidi Hongisto, Antti Jylhä, Janika Nättinen, Jochen Rieck, Tanja Ilmarinen, Zoltán Veréb, Ulla Aapola, Roger Beuerman, Goran Petrovski, Hannu Uusitalo, Heli Skottman
Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) provide an unlimited cell source for retinal cell replacement therapies. Clinical trials using hESC-RPE to treat diseases such as age-related macular degeneration (AMD) are currently underway. Human ESC-RPE cells have been thoroughly characterized at the gene level but their protein expression profile has not been studied at larger scale. In this study, proteomic analysis was used to compare hESC-RPE cells differentiated from two independent hESC lines, to primary human RPE (hRPE) using Isobaric tags for relative quantitation (iTRAQ)...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28727910/hla-b-40-01-45-a-novel-variant-of-hla-b-40-01-discovered-in-a-taiwanese-hematopoitic-stem-cell-donor
#8
K L Yang, P Y Lin
Two nucleotide replacements within HLA-B*40:01:01 result in the novel allele, HLA-B*40:01:45.
July 20, 2017: HLA
https://www.readbyqxmd.com/read/28726331/novel-fluid-shear-based-dissociation-device-for-improved-single-cell-dissociation-of-spheroids-and-cell-aggregates
#9
Ursula L Triantafillu, Jaron N Nix, Yonghyun Kim
Biological industries commonly rely on bioreactor systems for the large-scale production of cells. Cell aggregation, clumping, and spheroid morphology of certain suspension cells make their large-scale culture challenging. Growing stem cells as spheroids is indispensable to retain their stemness, but large spheroids (>500 µm diameter) suffer from poor oxygen and nutrient diffusion, ultimately resulting in premature cell death in the centers of the spheroids. Despite this, most large-scale bioprocesses do not have an efficient method for dissociating cells into single cells, but rely on costly enzymatic dissociation techniques...
July 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28726153/immunological-issues-after-stem-cell-based-%C3%AE-cell-replacement
#10
REVIEW
Valeria Sordi, Silvia Pellegrini, Lorenzo Piemonti
PURPOSE OF REVIEW: Islet and pancreas transplantation prove that β cell replacement can cure the glycemic derangements in type 1 diabetes (T1D). Induced pluripotent stem cells (iPSCs) can differentiate into functional insulin-producing cells, able to restore normoglycemia in diabetic animal models. iPSCs in particular can be derived from the somatic cells of a person with T1D. This review aims to clarify if it is possible to transplant autologous iPSC-derived β cells without immunosuppression or which are the alternative approaches...
September 2017: Current Diabetes Reports
https://www.readbyqxmd.com/read/28725655/cell-replacement-therapy-is-the-remedial-solution-for-treating-parkinson-s-disease
#11
REVIEW
Venkatesan Dhivya, Vellingiri Balachandar
The selective degeneration of dopaminergic (DA) neurons in Parkinson's disease (PD) has made an idol target for cell replacement therapies and other emerging surgical treatments. Certainly, by transplantation method, the therapeutic regimens such as human fetal ventral midbrain (hfVM) cells, human embryonic stem cells (hESCs), human neural stem/precursor/ progenitor cells (hNSCs/hNPCs), human mesenchymal stem cells (hMSCs), human induced neural stem cells (hiNSCs), and human induced pluripotent stem cells (hiPSCs) have been used into DA deficient striatum...
2017: Stem Cell Investigation
https://www.readbyqxmd.com/read/28724453/decrease-in-vancomycin-resistant-enterococcus-colonization-after-extensive-renovation-of-a-unit-dedicated-to-the-treatment-of-hematologic-malignancies-and-hematopoietic-stem-cell-transplantation
#12
Clyde D Ford, Michaela A Gazdik Stofer, Jana Coombs, Bert K Lopansri, Brandon J Webb, Gabriela Motyckova, Finn Bo Petersen
OBJECTIVE While a direct relation between hospital construction and concomitant infection rates has been clearly established, few data are available regarding the environmental decontamination effects of renovation in which surfaces are replaced and regarding subsequent infection incidence. DESIGN Retrospective clinical study with vancomycin-resistant Enterococcus (VRE) molecular strain typing and environmental cultures. SETTING A regional referral center for acute leukemia and hematopoietic stem-cell transplantation...
July 20, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28719101/neuronal-degeneration-and-regeneration-induced-by-axotomy-in-the-olfactory-epithelium-of-xenopus-laevis
#13
A S Cervino, D A Paz, J L Frontera
The olfactory epithelium (OE) has the remarkable capability to constantly replace olfactory receptor neurons (ORNs) due to the presence of neural stem cells (NSCs). For this reason, the OE provides an excellent model to study neurogenesis and neuronal differentiation. In the present work, we induced neuronal degeneration in the OE of Xenopus laevis larvae by bilateral axotomy of the olfactory nerves. We found that axotomy induces specific- neuronal death through apoptosis between 24 and 48h post-injury. In concordance, there was a progressive decrease of the mature-ORN marker OMP until it was completely absent 72h post-injury...
July 18, 2017: Developmental Neurobiology
https://www.readbyqxmd.com/read/28715851/paradigm-shifts-peregrinations-and-pixies-in-ophthalmology
#14
REVIEW
A O Minas Coroneo
Defending vision, our highest bandwidth sense, challenges human ingenuity. Replacing or repairing highly specialized but scarce tissue within constraints of transparency, tissue shape and alignment, tissue borders and pressure maintenance, presents special challenges. Many, mostly destructive surgical procedures, were developed prior to an understanding of underlying pathophysiology. For a number of conditions, both reconstructive and destructive procedures co-exist yet there are few guidelines as to the better approach...
July 17, 2017: Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/28713606/autologous-concentrated-bone-marrow-grafting-for-the-treatment-of-osteonecrosis-of-the-humeral-head-a-report-of-five-shoulders-in-four-cases
#15
Takeshi Makihara, Tomokazu Yoshioka, Hisashi Sugaya, Masashi Yamazaki, Hajime Mishima
Five shoulders in four patients affected by advanced osteonecrosis of the humeral head were treated with autologous concentrated bone marrow grafting. Bone marrow sample was aspirated from the iliac crests, concentrated by a centrifugation technique, and injected into the necrotic site. The shoulders were evaluated radiologically with X-ray scoring and clinically with measurement of range of motion and pain score (visual analogue scale, VAS). The mean follow-up period was 49.4 (range, 24-73) months. The concentration ratio of nucleated cells was calculated and the number of transplanted mesenchymal stem cells (MSC) was estimated by a colony-forming assay...
2017: Case Reports in Orthopedics
https://www.readbyqxmd.com/read/28709842/what-compatibility-in-2017-for-the-haematopoietic-stem-cell-transplantation
#16
X Lafarge
The diversification of potential donors to perform stem cell allografts now enables to propose a compatible graft cell source adapted to the different clinical situations. Transplants with a geno-identical sibling donor, otherwise with the most HLA-compatible unrelated donor, remain the first-line solutions. Alternative transplants allow to graft patients having no donors in international registries, owing to the rarity of their HLA typing. They are carried out with fairly incompatible grafts and are therefore limited by the existence in the recipient of preformed anti-HLA antibodies which predispose to their rejection...
July 11, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/28705258/fate-bias-during-neural-regeneration-adjusts-dynamically-without-recapitulating-developmental-fate-progression
#17
Jeremy Ng Chi Kei, Peter David Currie, Patricia Regina Jusuf
BACKGROUND: Regeneration of neurons in the central nervous system is poor in humans. In other vertebrates neural regeneration does occur efficiently and involves reactivation of developmental processes. Within the neural retina of zebrafish, Müller glia are the main stem cell source and are capable of generating progenitors to replace lost neurons after injury. However, it remains largely unknown to what extent Müller glia and neuron differentiation mirror development. METHODS: Following neural ablation in the zebrafish retina, dividing cells were tracked using a prolonged labelling technique...
July 13, 2017: Neural Development
https://www.readbyqxmd.com/read/28702321/a-high-content-small-molecule-screen-identifies-novel-inducers-of-definitive-endoderm
#18
Alexander Korostylev, Pallavi U Mahaddalkar, Oliver Keminer, Kamyar Hadian, Kenji Schorpp, Philip Gribbon, Heiko Lickert
OBJECTIVES: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) can generate any given cell type in the human body. One challenge for cell-replacement therapy is the efficient differentiation and expansion of large quantities of progenitor cells from pluripotent stem cells produced under good manufacturing practice (GMP). FOXA2 and SOX17 double positive definitive endoderm (DE) progenitor cells can give rise to all endoderm-derived cell types in the thymus, thyroid, lung, pancreas, liver, and gastrointestinal tract...
July 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28699526/hydrogel-based-cell-therapies-for-kidney-regeneration-current-trends-in-biofabrication-and-in-vivo-repair
#19
Katja Jansen, Carl C L Schuurmans, Jitske Jansen, Rosalinde Masereeuw, Tina Vermonden
Facing the problems of limited renal regeneration capacity and the persistent shortage of donor kidneys, dialysis remains the only treatment option for many end-stage renal disease patients. Unfortunately, dialysis is only a medium-term solution because large and protein-bound uremic solutes are not efficiently cleared from the body and lead to disease progression over time. Current strategies for improved renal replacement therapies (RRTs) range from whole organ engineering to biofabrication of renal assist devices and biological injectables for in vivo regeneration...
July 10, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28698932/small-molecules-for-reprogramming-and-transdifferentiation
#20
REVIEW
Hua Qin, Andong Zhao, Xiaobing Fu
Pluripotency reprogramming and transdifferentiation induced by transcription factors can generate induced pluripotent stem cells, adult stem cells or specialized cells. However, the induction efficiency and the reintroduction of exogenous genes limit their translation into clinical applications. Small molecules that target signaling pathways, epigenetic modifications, or metabolic processes can regulate cell development, cell fate, and function. In the recent decade, small molecules have been widely used in reprogramming and transdifferentiation fields, which can promote the induction efficiency, replace exogenous genes, or even induce cell fate conversion alone...
July 11, 2017: Cellular and Molecular Life Sciences: CMLS
keyword
keyword
22424
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"