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JOURNAL ARTICLE
Tong Sun, Yamin Lin, Yun Yu, Siqi Gao, Xingen Gao, Hongyi Zhang, Kecan Lin, Juqiang Lin
BACKGROUND: Surface-enhanced Raman scattering (SERS) technology have unique advantages of rapid, simple, and highly sensitive in the detection of serum, it can be used for the detection of liver cancer. However, some protein biomarkers in body fluids are often present at ultra-low concentrations and severely interfered with by the high-abundance proteins (HAPs), which will affect the detection of specificity and accuracy in cancer screening based on the SERS immunoassay. Clearly, there is a need for an unlabeled SERS method based on low abundance proteins, which is rapid, noninvasive, and capable of high precision detection and screening of liver cancer...
May 22, 2024: Analytica Chimica Acta
#2
JOURNAL ARTICLE
Kyung Hyun Boo, Jin Woo Kim, Minkyung Song
Adiponectin, a crucial protein hormone originating from adipose tissue, regulates key metabolic processes, including lipid metabolism, mitochondrial activity, and insulin sensitivity. These pleiotropic roles of adiponectin, along with its inverse correlation with metabolic disorders such as obesity, type II diabetes, and atherosclerosis, establish this protein as a potential therapeutic target. However, due to this complexity, challenges have arisen in its production with a natural conformation in bacterial or mammalian expression systems, hindering clinical translation...
March 30, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
#3
JOURNAL ARTICLE
April Kennedy, Gabriel Ma, Roozbeh Manshaei, Rebekah K Jobling, Raymond H Kim, Tamorah Lewis, Iris Cohn
Commercial pharmacogenetic testing panels capture a fraction of the genetic variation underlying medication metabolism and predisposition to adverse reactions. In this study we compared variation in six pharmacogenes detected by whole genome sequencing (WGS) to a targeted commercial panel in a cohort of 308 individuals with family history of pediatric heart disease. In 1% of the cohort, WGS identified rare variants that altered the interpretation of metabolizer status and would thus prevent potential errors in gene-based dosing...
February 22, 2024: NPJ Genomic Medicine
#4
JOURNAL ARTICLE
Wen You, Asal Pilehvari, Ruoding Shi, Wendy Cohn, Christina Sheffield, Philip I-Fon Chow, Becca Anne Krukowski, Roger Anderson
BACKGROUND: Existing financial hardship screening does not capture the multifaceted and dynamic nature of the problem. The use of existing health system data is a promising way to enable scalable and sustainable financial hardship screening. METHODS: We used existing data from 303 adult patients with cancer at the University of Virginia Comprehensive Cancer Center (2016-2018). All received distress screening and had a valid financial assistance screening based solely on household size-adjusted income...
November 21, 2023: Cancer Medicine
#5
JOURNAL ARTICLE
Guntur Berlian, Catur Riani, Neng Fisheri Kurniati, Heni Rachmawati
As one of the most popular sources for fish albumin, Channa striata has been considered as a promising substitute for human albumin. However, scientific information regarding its genomic and proteomic is very limited, making its identification rather complicated. In this study, we aimed to isolate, characterize, and examine the bioactivity of protein and peptide derivatives of C. striata albumin. Fractionation of albumin from C. striata extract was conducted using Cohn Process and the yield was evaluated. The peptides were further produced by enzymatic hydrolysis...
May 2023: Heliyon
#6
REVIEW
Albert Farrugia
Chronic recipients of plasma products are at risk of infection from blood-borne pathogens as a result of their inevitable exposure to agents which will contaminate a plasma manufacturing pool made up of thousands of individual donations. The generation of such a pool is an essential part of the large-scale manufacture of these products and is required for good manufacturing practice (GMP). Early observations of the transmission of hepatitis by pooled plasma and serum led to the incorporation of heat treatment of the albumin solution produced by industrial Cohn fractionation of plasma...
February 15, 2023: Pathogens
#7
JOURNAL ARTICLE
Lawrence M Schopfer, Emilie David, Steven H Hinrichs, Oksana Lockridge
Protection from the toxicity of nerve agents is achieved by pretreatment with human butyrylcholinesterase (BChE). Current methods for purifying large quantities of BChE from frozen Cohn fraction IV-4 produce 99% pure enzyme, but the yield is low (21%). Our goal was to simplify the purification procedure and increase the yield. Butyrylcholinesterase was extracted from frozen Cohn fraction IV-4 in 10 volumes of water pH 6. The filtered extract was pumped onto a Hupresin affinity column. The previously utilized anion exchange chromatography step was omitted...
2023: PloS One
#8
JOURNAL ARTICLE
Arash Jamshidi, Minetta C Liu, Eric A Klein, Oliver Venn, Earl Hubbell, John F Beausang, Samuel Gross, Collin Melton, Alexander P Fields, Qinwen Liu, Nan Zhang, Eric T Fung, Kathryn N Kurtzman, Hamed Amini, Craig Betts, Daniel Civello, Peter Freese, Robert Calef, Konstantin Davydov, Saniya Fayzullina, Chenlu Hou, Roger Jiang, Byoungsok Jung, Susan Tang, Vasiliki Demas, Joshua Newman, Onur Sakarya, Eric Scott, Archana Shenoy, Seyedmehdi Shojaee, Kristan K Steffen, Virgil Nicula, Tom C Chien, Siddhartha Bagaria, Nathan Hunkapiller, Mohini Desai, Zhao Dong, Donald A Richards, Timothy J Yeatman, Allen L Cohn, David D Thiel, Donald A Berry, Mohan K Tummala, Kristi McIntyre, Mikkael A Sekeres, Alan Bryce, Alexander M Aravanis, Michael V Seiden, Charles Swanton
In the Circulating Cell-free Genome Atlas (NCT02889978) substudy 1, we evaluate several approaches for a circulating cell-free DNA (cfDNA)-based multi-cancer early detection (MCED) test by defining clinical limit of detection (LOD) based on circulating tumor allele fraction (cTAF), enabling performance comparisons. Among 10 machine-learning classifiers trained on the same samples and independently validated, when evaluated at 98% specificity, those using whole-genome (WG) methylation, single nucleotide variants with paired white blood cell background removal, and combined scores from classifiers evaluated in this study show the highest cancer signal detection sensitivities...
December 12, 2022: Cancer Cell
#9
JOURNAL ARTICLE
Nadia Khan, Magdalena Kurnik-Łucka, Gniewomir Latacz, Krzysztof Gil, Sheikh Arshad Saeed
Platelet aggregation contributes to the pathogenesis of cardiovascular diseases. After activation it leads to dense granule secretion and 5-HT release. The question arises; how platelet aggregation is endogenously controlled during blood circulation. In preliminary studies, we observed that human platelets aggregate more rapidly when suspended in buffer as compared to those suspended in plasma (PRP). These observations point to the presence of an endogenous substance that may inhibit arachidonic acid- induced platelet aggregation...
September 15, 2022: Folia Medica Cracoviensia
#10
JOURNAL ARTICLE
Prabodh Sadana, Melissa Edler, Mirjavid Aghayev, Andrea Arias-Alvarado, Emilie Cohn, Serguei Ilchenko, Helen Piontkivska, Jagan A Pillai, Sangeeta Kashyap, Takhar Kasumov
Several aspects of diabetes pathophysiology and complications result from hyperglycemia-induced alterations in the structure and function of plasma proteins. Furthermore, insulin has a significant influence on protein metabolism by affecting both the synthesis and degradation of proteins in various tissues. To understand the role of progressive hyperglycemia on plasma proteins, in this study, we measured the turnover rates of high-density lipoprotein (HDL)-associated proteins in control (chow diet), prediabetic (a high-fat diet [HFD] for eight weeks) or diabetic (HFD for eight weeks with low-dose streptozotocin [HFD+STZ] in weeks 5-8 of HFD) C57BL/6J mice using heavy water (2 H2 O)-based metabolic labeling approach...
October 12, 2022: American Journal of Physiology. Endocrinology and Metabolism
#11
JOURNAL ARTICLE
Chaoji Huangfu, Nan Tang, Xiaokun Yang, Zhanwei Gong, Junzheng Li, Junting Jia, Jingang Zhang, Yan Huang, Yuyuan Ma
CONTEXT: α2-Macroglobulin (α2-M) is believed to be a potential anti-irradiation agent, but related mechanisms remains unclear. OBJECTIVE: We investigated the irradiation protective effect of α2-M. MATERIALS AND METHODS: A total of 10 Gy dose of irradiation was used to damage human skin fibroblasts. The influence of α2-M (100 µg/mL) on the proliferation, migration, invasion and apoptosis of fibroblasts was observed using Cell Counting Kit-8 (CCK8), wound healing, transwell, and flow cytometry...
December 2022: Pharmaceutical Biology
#12
JOURNAL ARTICLE
Elzbieta Lachert, Joanna Lasocka, Artur Bielawski, Ewa Sulkowska, Katarzyna Guz, Krzysztof Pyrc, Agnieszka Dabrowska, Agata Wawryniuk-Malmon, Magdalena Letowska, Krzysztof Tomasiewicz, Piotr Grabarczyk
This study aims to characterize the intermediates, and the final product (FP) obtained during the production of human intramuscular hyperimmune gamma globulin anti-SARS-CoV-2 (hIHGG anti-SARS-CoV-2) and to determine its stability. Material and methods : hIHGG anti-SARS-CoV-2 was fractionated from 270 convalescent plasma donations with the Cohn method. Prior to fractionation, the plasma was inactivated (Theraflex MB Plasma). Samples were defined using enzyme immunoassays (EIA) for anti-S1, anti-RBD S1, and anti-N antibodies, and neutralization assays with SARS-CoV-2 (VN) and pseudoviruses (PVN, decorated with SARS-CoV-2 S protein)...
June 17, 2022: Viruses
#13
JOURNAL ARTICLE
Javad Ghorbani, Peter D Wentzell, Mohsen Kompany-Zareh, Nematollah Omidikia
A two steps proposal for the purification of immunoglobulin G from human blood plasma is investigated. The first step is precipitation using cold ethanol based on the Cohn method with some modification and the second step is a chromatographic separation by DEAE-Sepharose FF resin as a weak anion exchanger. The presence of interferent in the region3 of chromatographic fractions, which is co-eluted with IgG, restricts the application of the mechanistic chromatography model. Therefore, multivariate cure resolution-alternating least squares (MCR-ALS) as a soft method is employed on measured absorbance data matrix from eluted fractions to recover pure concentration and spectral profiles...
July 19, 2022: Journal of Chromatography. A
#14
JOURNAL ARTICLE
Giuseppe Vergaro, Francesco Gentile, Alberto Aimo, James L Januzzi, A Mark Richards, Carolyn S P Lam, Rudolf A de Boer, Laura M G Meems, Roberto Latini, Lidia Staszewsky, Inder S Anand, Jay N Cohn, Thor Ueland, Lars Gullestad, Pål Aukrust, Hans-Peter Brunner-La Rocca, Antoni Bayes-Genis, Josep Lupón, Akiomi Yoshihisa, Yasuchika Takeishi, Michael Egstrup, Ida Gustafsson, Hanna K Gaggin, Kai M Eggers, Kurt Huber, Greg D Gamble, Lieng H Ling, Kui Toh Gerard Leong, Poh Shuah Daniel Yeo, Hean Yee Ong, Fazlur Jaufeerally, Tze P Ng, Richard Troughton, Robert N Doughty, Gerry Devlin, Mayanna Lund, Alberto Giannoni, Claudio Passino, Michele Emdin
AIMS: To define plasma concentrations, determinants, and optimal prognostic cut-offs of soluble suppression of tumorigenesis-2 (sST2), high-sensitivity cardiac troponin T (hs-cTnT), and N-terminal pro-B-type natriuretic peptide (NT-proBNP) in women and men with chronic heart failure (HF). METHODS AND RESULTS: Individual data of patients from the Biomarkers In Heart Failure Outpatient Study (BIOS) Consortium with sST2, hs-cTnT, and NT-proBNP measured were analysed...
August 2022: ESC Heart Failure
#15
JOURNAL ARTICLE
Raphael Schuster, Pascal Steffen, Benjamin Dreyer, Sascha Rohn, Hartmut Schlüter, Maria Riedner
Urotensin II (UII) and UII-related peptide (URP) are vasoactive peptide hormones causing strong vasoconstriction or vasodilation, depending on the type of blood vessel. In humans, the active forms are resulting from proteolytic cleavage of their inactive precursor protein. In blood plasma, a defined protease converting the inactive UII and URP precursors into their active forms has not been identified yet. Using mass spectrometry-based enzyme screening for detecting UII- and URP-converting enzymes, the human plasma fraction Cohn IV-4 was chromatographed, and the resulting fractions were screened for UII- or URP-generating activity...
November 4, 2021: Journal of Proteome Research
#16
JOURNAL ARTICLE
David W Haas, Rosie Mngqibisa, Jose Francis, Helen McIlleron, Jennifer A Robinson, Michelle A Kendall, Paxton Baker, Sajeeda Mawlana, Sharlaa Badal-Faesen, Francis Angira, Ayotunde Omoz-Oarhe, Wadzanai P Samaneka, Paolo Denti, Susan E Cohn
OBJECTIVE: In AIDS Clinical Trials Group study A5338, concomitant rifampicin, isoniazid, and efavirenz was associated with more rapid plasma medroxyprogesterone acetate (MPA) clearance compared to historical controls without tuberculosis or HIV therapy. We characterized the pharmacogenetics of this interaction. METHODS: In A5338, women receiving efavirenz-based HIV therapy and rifampicin plus isoniazid for tuberculosis underwent pharmacokinetic evaluations over 12 weeks following a 150-mg intramuscular injection of depot MPA...
August 6, 2021: Pharmacogenetics and Genomics
#17
JOURNAL ARTICLE
Giuseppe Vergaro, Francesco Gentile, Laura M G Meems, Alberto Aimo, James L Januzzi, A Mark Richards, Carolyn S P Lam, Roberto Latini, Lidia Staszewsky, Inder S Anand, Jay N Cohn, Thor Ueland, Lars Gullestad, Pål Aukrust, Hans-Peter Brunner-La Rocca, Antoni Bayes-Genis, Josep Lupón, Akiomi Yoshihisa, Yasuchika Takeishi, Michael Egstrup, Ida Gustafsson, Hanna K Gaggin, Kai M Eggers, Kurt Huber, Greg D Gamble, Lieng H Ling, Kui Tong Gerard Leong, Poh Shuah Daniel Yeo, Hean Yee Ong, Fazlur Jaufeerally, Tze P Ng, Richard Troughton, Robert N Doughty, Gerry Devlin, Mayanna Lund, Alberto Giannoni, Claudio Passino, Rudolf A de Boer, Michele Emdin
OBJECTIVES: The goal of this study was to assess the predictive power of N-terminal pro-B-type natriuretic peptide (NT-proBNP) and the decision cutoffs in heart failure (HF) across body mass index (BMI) categories. BACKGROUND: Concentrations of NT-proBNP predict outcome in HF. Although the influence of BMI to reduce levels of NT-proBNP is known, the impact of obesity on prognostic value remains uncertain. METHODS: Individual data from the BIOS (Biomarkers In Heart Failure Outpatient Study) consortium were analyzed...
June 29, 2021: JACC. Heart Failure
#18
JOURNAL ARTICLE
Ivan S Pires, Krianthan Govender, Carlos J Munoz, Alexander T Williams, Quintin T O'Boyle, Chintan Savla, Pedro Cabrales, Andre F Palmer
BACKGROUND: Hemolysis releases toxic cell-free hemoglobin (Hb), heme, and iron, which overwhelm their natural scavenging mechanisms during acute or chronic hemolytic conditions. This study describes a novel strategy to purify a protein cocktail containing a comprehensive set of scavenger proteins for potential treatment of hemolysis byproducts. STUDY DESIGN AND METHODS: Tangential flow filtration was used to purify a protein cocktail from Human Cohn Fraction IV (FIV)...
June 2021: Transfusion
#19
JOURNAL ARTICLE
Nick Marcks, Alberto Aimo, James L Januzzi, Giuseppe Vergaro, Aldo Clerico, Roberto Latini, Jennifer Meessen, Inder S Anand, Jay N Cohn, Jørgen Gravning, Thor Ueland, Antoni Bayes-Genis, Josep Lupón, Rudolf A de Boer, Akiomi Yoshihisa, Yasuchika Takeishi, Michael Egstrup, Ida Gustafsson, Hanna K Gaggin, Kai M Eggers, Kurt Huber, Ioannis Tentzeris, Andrea Ripoli, Claudio Passino, Sandra Sanders-van Wijk, Michele Emdin, Hans-Peter Brunner-La Rocca
BACKGROUND: Higher body mass index (BMI) is associated with better outcome compared with normal weight in patients with HF and other chronic diseases. It remains uncertain whether the apparent protective role of obesity relates to the absence of comorbidities. Therefore, we investigated the effect of BMI on outcome in younger patients without co-morbidities as compared to older patients with co-morbidities in a large heart failure (HF) population. METHODS: In an individual patient data analysis from pooled cohorts, 5,819 patients with chronic HF and data available on BMI, co-morbidities and outcome were analysed...
August 2021: Clinical Research in Cardiology: Official Journal of the German Cardiac Society
#20
JOURNAL ARTICLE
Daniele Focosi, Marco Tuccori, Massimo Franchini
Effective treatments specific for COVID-19 are still lacking. In the setting of passive immunotherapies based on neutralizing antibodies (nAbs), randomized controlled trials of COVID-19 convalescent plasma (CCP) anti-SARS-CoV-2 Spike protein monoclonal antibodies (mAb), which have been granted emergency use authorization, have suggested benefit in early disease course (less than 72 hours from symptoms and seronegative). Meanwhile, polyclonal immunoglobulins (i.e., hyperimmune serum), derived either from CCP donations or from animals immunized with SARS-CoV-2 antigens, are likely to become the next nAb-derived candidate...
February 15, 2021: Life
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