Nicolas Veland, Hannah J Gleneadie, Karen E Brown, Alessandro Sardini, Joaquim Pombo, Andrew Dimond, Vanessa Burns, Karen Sarkisyan, Chris Schiering, Zoe Webster, Matthias Merkenschlager, Amanda G Fisher
Aryl hydrocarbon receptor (AHR) signalling integrates biological processes that sense and respond to environmental, dietary, and metabolic challenges to ensure tissue homeostasis. AHR is a transcription factor that is inactive in the cytosol but upon encounter with ligand translocates to the nucleus and drives the expression of AHR targets, including genes of the cytochrome P4501 family of enzymes such as Cyp1a1. To dynamically visualise AHR activity in vivo, we generated reporter mice in which firefly luciferase (Fluc) was non-disruptively targeted into the endogenous Cyp1a1 locus...
April 10, 2024: Communications Biology