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https://www.readbyqxmd.com/read/29453057/mammarenaviruses-deleted-from-their-z-gene-are-replicative-and-produce-an-infectious-progeny-in-bhk-21-cells
#1
Amélie D Zaza, Cécile H Herbreteau, Christophe N Peyrefitte, Sébastien F Emonet
Mammarenaviruses bud out of infected cells via the recruitment of the endosomal sorting complex required for transport through late domain motifs localized into their Z protein. Here, we demonstrated that mammarenaviruses lacking this protein can be rescued and are replicative, despite a 3-log reduction in virion production, in BHK-21 cells, but not in five other cell lines. Mutations of putative late domain motifs identified into the viral nucleoprotein resulted in the almost complete abolition of infectious virion production by Z-deleted mammarenaviruses...
February 13, 2018: Virology
https://www.readbyqxmd.com/read/29439402/mammarenaviral-infection-is-dependent-on-directional-exposure-to-and-release-from-polarized-intestinal-epithelia
#2
Nikole L Warner, Jenny D Jokinen, Juliane I Beier, Kevin J Sokoloski, Igor S Lukashevich
Mammarenavirusesare single-stranded RNA viruses with a bisegmented ambisense genome. Ingestion has been shown as a natural route of transmission for both Lassa virus (LASV) and Lymphocytic choriomeningitis virus (LCMV). Due to the mechanism of transmission, epithelial tissues are among the first host cells to come in contact with the viruses, and as such they potentially play a role in spread of virus to naïve hosts. The role of the intestinal epithelia during arenavirus infection remains to be uncharacterized...
February 10, 2018: Viruses
https://www.readbyqxmd.com/read/29430345/design-of-peptide-based-epitope-vaccine-and-further-binding-site-scrutiny-led-to-groundswell-in-drug-discovery-against-lassa-virus
#3
Mohammad Uzzal Hossain, Taimur Md Omar, Arafat Rahman Oany, K M Kaderi Kibria, Abu Zaffar Shibly, Md Moniruzzaman, Syed Raju Ali, Md Monirul Islam
Lassa virus (LASV) is responsible for an acute viral hemorrhagic fever known as Lassa fever. Sequence analyses of LASV proteome identified the most immunogenic protein that led to predict both T-cell and B-cell epitopes and further target and binding site depiction could allow novel drug findings for drug discovery field against this virus. To induce both humoral and cell-mediated immunity peptide sequence SSNLYKGVY, conserved region 41-49 amino acids were found as the most potential B-cell and T-cell epitopes, respectively...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29295909/lamp1-increases-the-efficiency-of-lassa-virus-infection-by-promoting-fusion-in-less-acidic-endosomal-compartments
#4
Christine E Hulseberg, Lucie Fénéant, Katarzyna M Szymańska, Judith M White
Lassa virus (LASV) is an arenavirus whose entry into host cells is mediated by a glycoprotein complex (GPC) comprised of a receptor binding subunit, GP1, a fusogenic transmembrane subunit, GP2, and a stable signal peptide. After receptor-mediated internalization, arenaviruses converge in the endocytic pathway, where they are thought to undergo low-pH-triggered, GPC-mediated fusion with a late endosome membrane. A unique feature of LASV entry is a pH-dependent switch from a primary cell surface receptor (α-dystroglycan) to an endosomal receptor, lysosomal-associated membrane protein (Lamp1)...
January 2, 2018: MBio
https://www.readbyqxmd.com/read/29287681/alphavirus-vector-based-replicon-particles-expressing-multivalent-cross-protective-lassa-virus-glycoproteins
#5
Min Wang, Jenny Jokinen, Irina Tretyakova, Peter Pushko, Igor S Lukashevich
Lassa virus (LASV) is the most prevalent rodent-borne arenavirus circulated in West Africa. With population at risk from Senegal to Nigeria, LASV causes Lassa fever and is responsible for thousands of deaths annually. High genetic diversity of LASV is one of the challenges for vaccine R&D. We developed multivalent virus-like particle vectors (VLPVs) derived from the human Venezuelan equine encephalitis TC-83 IND vaccine (VEEV) as the next generation of alphavirus-based bicistronic RNA replicon particles. The genes encoding VEEV structural proteins were replaced with LASV glycoproteins (GPC) from distantly related clades I and IV with individual 26S promoters...
December 26, 2017: Vaccine
https://www.readbyqxmd.com/read/29238121/epidemic-preparedness-and-management-a-guide-on-lassa-fever-outbreak-preparedness-plan
#6
REVIEW
Akinola Ayoola Fatiregun, Elvis Efe Isere
Epidemic prone diseases threaten public health security. These include diseases such as cholera, meningitis, and hemorrhagic fevers, especially Lassa fever for which Nigeria reports considerable morbidity and mortality annually. Interestingly, where emergency epidemic preparedness plans are in place, timely detection of outbreaks is followed by a prompt and appropriate response. Furthermore, due to the nature of spread of Lassa fever in an outbreak setting, there is the need for health-care workers to be familiar with the emerging epidemic management framework that has worked in other settings for effective preparedness and response...
January 2017: Nigerian Medical Journal: Journal of the Nigeria Medical Association
https://www.readbyqxmd.com/read/29237830/axl-can-serve-as-entry-factor-for-lassa-virus-depending-on-the-functional-glycosylation-of-dystroglycan
#7
Chiara Fedeli, Giulia Torriani, Clara Galan-Navarro, Marie-Laurence Moraz, Hector Moreno, Gisa Gerold, Stefan Kunz
Fatal infection with the highly pathogenic Lassa virus (LASV) is characterized by extensive viral dissemination, indicating broad tissue tropism. The major cellular receptor for LASV is the highly conserved extracellular matrix receptor dystroglycan (DG). Binding of LASV depends on DG's tissue-specific post-translational modification with the unusual O-linked polysaccharide matriglycan. Interestingly, functional glycosylation of DG does not always correlate with viral tropism observed in vivo The broadly expressed phosphatidylserine (PS) receptors Axl and Tyro3 were recently identified as alternative LASV receptor candidates...
December 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29186289/discharge-criteria-for-patient-with-lassa-fever-infection
#8
Emanuele Nicastri, Francesco Vairo, Giuseppe Ippolito
No abstract text is available yet for this article.
November 24, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29155659/us-federal-travel-restrictions-for-persons-with-higher-risk-exposures-to-communicable-diseases-of-public-health-concern
#9
Laura A Vonnahme, M Robynne Jungerman, Reena K Gulati, Petra Illig, Francisco Alvarado-Ramy
Published guidance recommends controlled movement for persons with higher-risk exposures (HREs) to communicable diseases of public health concern; US federal public health travel restrictions (PHTRs) might be implemented to enforce these measures. We describe persons eligible for and placed on PHTRs because of HREs during 2014-2016. There were 160 persons placed on PHTRs: 142 (89%) involved exposure to Ebola virus, 16 (10%) to Lassa fever virus, and 2 (1%) to Middle East respiratory syndrome coronavirus. Most (90%) HREs were related to an epidemic...
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29138016/the-challenges-of-detecting-and-responding-to-a-lassa-fever-outbreak-in-an-ebola-affected-setting
#10
E L Hamblion, P Raftery, A Wendland, E Dweh, G S Williams, R N C George, L Soro, V Katawera, P Clement, A N Gasasira, E Musa, T K Nagbe
OBJECTIVES: Lassa fever (LF), a priority emerging pathogen likely to cause major epidemics, is endemic in much of West Africa and is difficult to distinguish from other viral hemorrhagic fevers, including Ebola virus disease (EVD). Definitive diagnosis requires laboratory confirmation, which is not widely available in affected settings. The public health action to contain a LF outbreak and the challenges encountered in an EVD-affected setting are reported herein. METHODS: In February 2016, a rapid response team was deployed in Liberia in response to a cluster of LF cases...
January 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29136087/the-utility-and-perspectives-of-ngs-based-methods-in-bsl-3-and-bsl-4-laboratory-sequencing-and-analysis-strategies
#11
Tomasz Wolkowicz
Modern diagnostics is in general based on molecular biology methods. Nowadays sequencing-based methods, especially whole genome sequencing, are becoming increasingly important. Implementation of such methods into routine diagnostic of highly dangerous pathogens, like Bacillus anthracis, Francisella tularensis, Yersinia pestis, Ebola virus, MERS, Lassa virus etc. would be very helpful. The best diagnostic strategy would be the metagenomic sequencing directly from the clinical sample. Implementation of majority of currently available WGS platforms inside the BSL-3 or 4 laboratory is impractical because of the size of the equipment and time consuming wet lab part (e...
November 10, 2017: Briefings in Functional Genomics
https://www.readbyqxmd.com/read/29135337/dna-vaccines-elicit-durable-protective-immunity-against-individual-or-simultaneous-infections-with-lassa-and-ebola-viruses-in-guinea-pigs
#12
Kathleen A Cashman, Eric R Wilkinson, Suzanne E Wollen, Joshua D Shamblin, Justine M Zelko, Jeremy J Bearss, Xiankun Zeng, Kate E Broderick, Connie S Schmaljohn
We previously developed optimized DNA vaccines against both Lassa fever and Ebola hemorrhagic fever viruses and demonstrated that they were protective individually in guinea pig and nonhuman primate models. In this study, we vaccinated groups of strain 13 guinea pigs two times, four weeks apart with 50 µg of each DNA vaccine or a mock vaccine at discrete sites by intradermal electroporation. Five weeks following the second vaccinations, guinea pigs were exposed to lethal doses of Lassa virus, Ebola virus, or a combination of both viruses simultaneously...
November 14, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29126899/merimepodib-an-impdh-inhibitor-suppresses-replication-of-zika-virus-and-other-emerging-viral-pathogens
#13
Xiao Tong, Jeanon Smith, Natalya Bukreyeva, Takaaki Koma, John T Manning, Raj Kalkeri, Ann D Kwong, Slobodan Paessler
Zika virus (ZIKV), a member of the Flaviviridae family, has recently been linked to abnormal pregnancies, fetal death, microcephaly, and Guillain-Barré syndrome in humans. Merimepodib (MMPD, VX-497), a potent inhibitor of inosine-5'-monophosphate dehydrogenase (IMPDH), has shown antiviral activity against HCV and a variety of DNA and RNA viruses in vitro. In this report, we expand the antiviral spectrum of MMPD, and demonstrate that MMPD inhibits ZIKV RNA replication with an EC50 of 0.6 μM. Furthermore, MMPD reduces the virus production of ZIKV as well as several other important emerging viral pathogens such as Ebola, Lassa, Chikungunya, and Junin viruses...
January 2018: Antiviral Research
https://www.readbyqxmd.com/read/29083948/chimpanzee-adenoviral-vectors-as-vaccines-for-outbreak-pathogens
#14
Katie Ewer, Sarah Sebastian, Alexandra J Spencer, Sarah Gilbert, Adrian V S Hill, Teresa Lambe
The 2014-15 Ebola outbreak in West Africa highlighted the potential for large disease outbreaks caused by emerging pathogens and has generated considerable focus on preparedness for future epidemics. Here we discuss drivers, strategies and practical considerations for developing vaccines against outbreak pathogens. Chimpanzee adenoviral (ChAd) vectors have been developed as vaccine candidates for multiple infectious diseases and prostate cancer. ChAd vectors are safe and induce antigen-specific cellular and humoral immunity in all age groups, as well as circumventing the problem of pre-existing immunity encountered with human Ad vectors...
October 30, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29070682/epistastic-interactions-within-the-jun%C3%A3-n-virus-envelope-glycoprotein-complex-provide-an-evolutionary-barrier-to-reversion-in-the-live-attenuated-candid-1-vaccine
#15
Joanne York, Jack H Nunberg
The Candid#1 strain of Junín virus was developed using a conventional attenuation strategy of serial passage in non-host animals and cultured cells. The live-attenuated Candid#1 vaccine is used in Argentina to protect at-risk individuals against Argentine hemorrhagic fever, but has not been licensed in the United States. Recent studies have revealed that Candid#1 attenuation is entirely dependent on a phenylalanine-to-isoleucine substitution at position 427 in the fusion subunit (GP2) of the viral envelope glycoprotein complex (GPC), thereby raising concerns regarding the potential for reversion to virulence...
October 25, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29045192/a-dna-vaccine-delivered-by-dermal-electroporation-fully-protects-cynomolgus-macaques-against-lassa-fever
#16
Kathleen A Cashman, Eric R Wilkinson, Carl I Shaia, Paul R Facemire, Todd M Bell, Jeremy J Bearss, Joshua D Shamblin, Suzanne E Wollen, Kate E Broderick, Niranjan Y Sardesai, Connie S Schmaljohn
Lassa virus (LASV) is an ambisense RNA virus in the Arenaviridae family and is the etiological agent of Lassa fever, a severe hemorrhagic disease endemic to West and Central Africa. (1,2) There are no US Food and Drug Administration (FDA)-licensed vaccines available to prevent Lassa fever. (1,2) in our previous studies, we developed a gene-optimized DNA vaccine that encodes the glycoprotein precursor gene of LASV (Josiah strain) and demonstrated that 3 vaccinations accompanied by dermal electroporation protected guinea pigs from LASV-associated illness and death...
October 18, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29031848/local-national-and-regional-viral-haemorrhagic-fever-pandemic-potential-in-africa-a-multistage-analysis
#17
David M Pigott, Aniruddha Deshpande, Ian Letourneau, Chloe Morozoff, Robert C Reiner, Moritz U G Kraemer, Shannon E Brent, Isaac I Bogoch, Kamran Khan, Molly H Biehl, Roy Burstein, Lucas Earl, Nancy Fullman, Jane P Messina, Adrian Q N Mylne, Catherine L Moyes, Freya M Shearer, Samir Bhatt, Oliver J Brady, Peter W Gething, Daniel J Weiss, Andrew J Tatem, Luke Caley, Tom De Groeve, Luca Vernaccini, Nick Golding, Peter Horby, Jens H Kuhn, Sandra J Laney, Edmond Ng, Peter Piot, Osman Sankoh, Christopher J L Murray, Simon I Hay
BACKGROUND: Predicting when and where pathogens will emerge is difficult, yet, as shown by the recent Ebola and Zika epidemics, effective and timely responses are key. It is therefore crucial to transition from reactive to proactive responses for these pathogens. To better identify priorities for outbreak mitigation and prevention, we developed a cohesive framework combining disparate methods and data sources, and assessed subnational pandemic potential for four viral haemorrhagic fevers in Africa, Crimean-Congo haemorrhagic fever, Ebola virus disease, Lassa fever, and Marburg virus disease...
December 16, 2017: Lancet
https://www.readbyqxmd.com/read/29019310/lessons-learnt-from-imported-cases-and-onward-transmission-of-lassa-fever-in-europe-support-broader-management-of-viral-haemorrhagic-fevers
#18
Roger Hewson
No abstract text is available yet for this article.
September 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29019309/management-of-a-lassa-fever-outbreak-rhineland-palatinate-germany-2016
#19
Lutz Ehlkes, Maja George, Gerhard Samosny, Florian Burckhardt, Manfred Vogt, Stefan Bent, Klaus Jahn, Philipp Zanger
Due to rapid diagnosis and isolation of imported cases, community outbreaks of viral haemorrhagic fevers (VHF) are considered unlikely in industrialised countries. In March 2016, the first documented locally acquired case of Lassa fever (LF) outside Africa occurred, demonstrating the disease's potential as a cross-border health threat. We describe the management surrounding this case of LF in Rhineland-Palatinate - the German federal state where secondary transmission occurred. Twelve days after having been exposed to the corpse of a LF case imported from Togo, a symptomatic undertaker tested positive for Lassa virus RNA...
September 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29019307/control-measures-following-a-case-of-imported-lassa-fever-from-togo-north-rhine-westphalia-germany-2016
#20
Clara Lehmann, Matthias Kochanek, Diana Abdulla, Stephan Becker, Boris Böll, Anne Bunte, Daniel Cadar, Arno Dormann, Markus Eickmann, Petra Emmerich, Torsten Feldt, Christina Frank, Jochen Fries, Martin Gabriel, Udo Goetsch, René Gottschalk, Stephan Günther, Michael Hallek, Dieter Häussinger, Christian Herzog, Björn Jensen, Felix Kolibay, Michael Krakau, Georg Langebartels, Toni Rieger, Lars Schaade, Jonas Schmidt-Chanasit, Edgar Schömig, Gundolf Schüttfort, Alexander Shimabukuro-Vornhagen, Michael von Bergwelt-Baildon, Ulrike Wieland, Gerhard Wiesmüller, Timo Wolf, Gerd Fätkenheuer
In a patient transferred from Togo to Cologne, Germany, Lassa fever was diagnosed 12 days post mortem. Sixty-two contacts in Cologne were categorised according to the level of exposure, and gradual infection control measures were applied. No clinical signs of Lassa virus infection or Lassa specific antibodies were observed in the 62 contacts. Thirty-three individuals had direct contact to blood, other body fluids or tissue of the patients. Notably, with standard precautions, no transmission occurred between the index patient and healthcare workers...
September 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
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