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https://www.readbyqxmd.com/read/29651117/field-validation-of-recombinant-antigen-immunoassays-for-diagnosis-of-lassa-fever
#1
Matthew L Boisen, Jessica N Hartnett, Jeffrey G Shaffer, Augustine Goba, Mambu Momoh, John Demby Sandi, Mohamed Fullah, Diana K S Nelson, Duane J Bush, Megan M Rowland, Megan L Heinrich, Anatoliy P Koval, Robert W Cross, Kayla G Barnes, Anna E Lachenauer, Aaron E Lin, Mahan Nekoui, Dylan Kotliar, Sarah M Winnicki, Katherine J Siddle, Michael Gbakie, Mbalu Fonnie, Veronica J Koroma, Lansana Kanneh, Peter C Kulakosky, Kathryn M Hastie, Russell B Wilson, Kristian G Andersen, Onikepe O Folarin, Christian T Happi, Pardis C Sabeti, Thomas W Geisbert, Erica Ollmann Saphire, S Humarr Khan, Donald S Grant, John S Schieffelin, Luis M Branco, Robert F Garry
Lassa fever, a hemorrhagic fever caused by Lassa virus (LASV), is endemic in West Africa. It is difficult to distinguish febrile illnesses that are common in West Africa from Lassa fever based solely on a patient's clinical presentation. The field performance of recombinant antigen-based Lassa fever immunoassays was compared to that of quantitative polymerase chain assays (qPCRs) using samples from subjects meeting the case definition of Lassa fever presenting to Kenema Government Hospital in Sierra Leone. The recombinant Lassa virus (ReLASV) enzyme-linked immunosorbant assay (ELISA) for detection of viral antigen in blood performed with 95% sensitivity and 97% specificity using a diagnostic standard that combined results of the immunoassays and qPCR...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29596412/development-and-evaluation-of-antibody-capture-immunoassays-for-detection-of-lassa-virus-nucleoprotein-specific-immunoglobulin-m-and-g
#2
Martin Gabriel, Donatus I Adomeh, Jacqueline Ehimuan, Jennifer Oyakhilome, Emmanuel O Omomoh, Yemisi Ighodalo, Thomas Olokor, Kofi Bonney, Meike Pahlmann, Petra Emmerich, Michaela Lelke, Linda Brunotte, Stephan Ölschläger, Corinna Thomé-Bolduan, Beate Becker-Ziaja, Carola Busch, Ikponmwonsa Odia, Ephraim Ogbaini-Emovon, Peter O Okokhere, Sylvanus A Okogbenin, George O Akpede, Herbert Schmitz, Danny A Asogun, Stephan Günther
BACKGROUND: The classical method for detection of Lassa virus-specific antibodies is the immunofluorescence assay (IFA) using virus-infected cells as antigen. However, IFA requires laboratories of biosafety level 4 for assay production and an experienced investigator to interpret the fluorescence signals. Therefore, we aimed to establish and evaluate enzyme-linked immunosorbent assays (ELISA) using recombinant Lassa virus nucleoprotein (NP) as antigen. METHODOLOGY/PRINCIPAL FINDINGS: The IgM ELISA is based on capturing IgM antibodies using anti-IgM, and the IgG ELISA is based on capturing IgG antibody-antigen complexes using rheumatoid factor or Fc gamma receptor CD32a...
March 29, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29593043/a-vaccine-platform-against-arenaviruses-based-on-a-recombinant-hyper-attenuated-mopeia-virus-expressing-heterologous-glycoproteins
#3
Xavier Carnec, Mathieu Matéo, Audrey Page, Stéphanie Reynard, Jimmy Hortion, Caroline Picard, Elsie Yekwa, François Ferron, Sylvain Baize
Several Old and New-World arenaviruses are responsible for severe endemic and epidemic hemorrhagic fevers, whereas other members of the Arenaviridae family are nonpathogenic. To date, no approved vaccines, antiviral or specific treatments are available, except for Junín virus. Protection in non-human primates against Lassa fever virus (LASV) is however possible through the inoculation of the closely related but nonpathogenic Mopeia virus (MOPV) before challenge with LASV. We reasoned that this virus, modified using reverse genetics, would represent the basis for the generation of a vaccine platform against LASV and other pathogenic arenaviruses...
March 28, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29593035/a-highly-conserved-leucine-in-mammarenavirus-matrix-z-protein-is-required-for-z-interaction-with-the-virus-l-polymerase-and-z-stability-in-cells-harboring-an-active-viral-ribonucleoprotein
#4
Masaharu Iwasaki, Juan C de la Torre
Mammarenaviruses cause chronic infections in their natural rodent hosts. Infected rodents shed infectious virus into excreta. Humans are infected through mucosal exposure to aerosols or direct contact of abraded skin with fomites, resulting in a wide-range of manifestations from asymptomatic or mild febrile illness to severe life-threatening hemorrhagic fever. The mammarenavirus matrix Z protein has been shown to be a main driving force of virus budding and to act as a negative regulator of viral RNA synthesis...
March 28, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29590055/nigeria-hit-by-unprecedented-lassa-fever-outbreak
#5
Leslie Roberts
No abstract text is available yet for this article.
March 16, 2018: Science
https://www.readbyqxmd.com/read/29582756/lassa-fever-and-global-health-security
#6
EDITORIAL
The Lancet Infectious Diseases
No abstract text is available yet for this article.
April 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/29581897/baseline-mapping-of-lassa-fever-virology-epidemiology-and-vaccine-research-and-development
#7
REVIEW
Hoai J Hallam, Steven Hallam, Sergio E Rodriguez, Alan D T Barrett, David W C Beasley, Arlene Chua, Thomas G Ksiazek, Gregg N Milligan, Vaseeharan Sathiyamoorthy, Lisa M Reece
Lassa fever (LF) is a zoonotic disease associated with acute and potentially fatal hemorrhagic illness caused by the Lassa virus (LASV), a member of the family Arenaviridae . It is generally assumed that a single infection with LASV will produce life-long protective immunity. This suggests that protective immunity induced by vaccination is an achievable goal and that cell-mediated immunity may play a more important role in protection, at least following natural infection. Seropositive individuals in endemic regions have been shown to have LASV-specific T cells recognizing epitopes for nucleocapsid protein (NP) and glycoprotein precursor (GPC), suggesting that these will be important vaccine immunogens...
2018: NPJ Vaccines
https://www.readbyqxmd.com/read/29572697/movement-patterns-of-small-rodents-in-lassa-fever-endemic-villages-in-guinea
#8
Joachim Mariën, Fodé Kourouma, N'Faly Magassouba, Herwig Leirs, Elisabeth Fichet-Calvet
The Natal multimammate mouse (Mastomys natalensis) is the reservoir host of Lassa arenavirus, the etiological agent of Lassa fever in humans. Because there exists no vaccine for human use, rodent control and adjusting human behavior are currently considered to be the only options for Lassa fever control. In order to develop efficient rodent control programs, more information about the host's ecology is needed. In this study, we investigated the spatial behavior of M. natalensis and other small rodents in two capture-mark-recapture and four dyed bait (Rhodamine B) experiments in Lassa fever-endemic villages in Upper Guinea...
March 23, 2018: EcoHealth
https://www.readbyqxmd.com/read/29565399/deadly-lassa-fever-outbreak-tests-nigeria-s-revamped-health-agency
#9
Amy Maxmen
No abstract text is available yet for this article.
March 22, 2018: Nature
https://www.readbyqxmd.com/read/29524445/favipiravir-as-a-potential-countermeasure-against-neglected-and-emerging-rna-viruses
#10
REVIEW
Leen Delang, Rana Abdelnabi, Johan Neyts
Favipiravir, also known as T-705, is an antiviral drug that has been approved in 2014 in Japan to treat pandemic influenza virus infections. The drug is converted intracellularly into its active, phosphoribosylated form, which is recognized as a substrate by the viral RNA-dependent RNA polymerase. Interestingly, besides its anti-influenza virus activity, this molecule is also able to inhibit the replication of flavi-, alpha-, filo-, bunya-, arena-, noro-, and of other RNA viruses, which include neglected and (re)emerging viruses for which no antiviral therapy is currently available...
March 7, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29523497/clinical-and-laboratory-predictors-of-lassa-fever-outcome-in-a-dedicated-treatment-facility-in-nigeria-a-retrospective-observational-cohort-study
#11
Peter Okokhere, Andres Colubri, Chukwuemeka Azubike, Christopher Iruolagbe, Omoregie Osazuwa, Shervin Tabrizi, Elizabeth Chin, Sara Asad, Ehi Ediale, Mojeed Rafiu, Donatus Adomeh, Ikponmwosa Odia, Rebecca Atafo, Chris Aire, Sylvanus Okogbenin, Meike Pahlman, Beate Becker-Ziaja, Danny Asogun, Terrence Fradet, Ben Fry, Stephen F Schaffner, Christian Happi, George Akpede, Stephan Günther, Pardis C Sabeti
BACKGROUND: Lassa fever is a viral haemorrhagic disease endemic to west Africa. No large-scale studies exist from Nigeria, where the Lassa virus (LASV) is most diverse. LASV diversity, coupled with host genetic and environmental factors, might cause differences in disease pathophysiology. Small-scale studies in Nigeria suggest that acute kidney injury is an important clinical feature and might be a determinant of survival. We aimed to establish the demographic, clinical, and laboratory factors associated with mortality in Nigerian patients with Lassa fever, and hypothesised that LASV was the direct cause of intrinsic renal damage for a subset of the patients with Lassa fever...
March 6, 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/29523495/predicting-outcome-and-improving-treatment-for-lassa-fever
#12
Thomas W Geisbert
No abstract text is available yet for this article.
March 6, 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/29507948/what-pediatricians-should-know-about-lassa-virus
#13
David Greenky, Barbara Knust, Eric J Dziuban
No abstract text is available yet for this article.
March 5, 2018: JAMA Pediatrics
https://www.readbyqxmd.com/read/29486937/lassa-fever-in-nigeria-the-great-unknown
#14
Talha Burki
No abstract text is available yet for this article.
February 24, 2018: Lancet
https://www.readbyqxmd.com/read/29470486/lassa-fever-induced-sensorineural-hearing-loss-a-neglected-public-health-and-social-burden
#15
REVIEW
Elizabeth J Mateer, Cheng Huang, Nathan Y Shehu, Slobodan Paessler
Although an association between Lassa fever (LF) and sudden-onset sensorineural hearing loss (SNHL) was confirmed clinically in 1990, the prevalence of LF-induced SNHL in endemic countries is still underestimated. LF, a viral hemorrhagic fever disease caused by Lassa virus (LASV), is endemic in West Africa, causing an estimated 500,000 cases and 5,000 deaths per year. Sudden-onset SNHL, one complication of LF, occurs in approximately one-third of survivors and constitutes a neglected public health and social burden...
February 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29462184/interactome-analysis-of-the-lymphocytic-choriomeningitis-virus-nucleoprotein-in-infected-cells-reveals-atpase-na-k-transporting-subunit-alpha-1-and-prohibitin-as-host-cell-factors-involved-in-the-life-cycle-of-mammarenaviruses
#16
Masaharu Iwasaki, Petra Minder, Yíngyún Caì, Jens H Kuhn, John R Yates, Bruce E Torbett, Juan C de la Torre
Several mammalian arenaviruses (mammarenaviruses) cause hemorrhagic fevers in humans and pose serious public health concerns in their endemic regions. Additionally, mounting evidence indicates that the worldwide-distributed, prototypic mammarenavirus, lymphocytic choriomeningitis virus (LCMV), is a neglected human pathogen of clinical significance. Concerns about human-pathogenic mammarenaviruses are exacerbated by of the lack of licensed vaccines, and current anti-mammarenavirus therapy is limited to off-label use of ribavirin that is only partially effective...
February 20, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29460758/new-lineage-of-lassa-virus-togo-2016
#17
Shannon L M Whitmer, Thomas Strecker, Daniel Cadar, Hans-Peter Dienes, Kelly Faber, Ketan Patel, Shelley M Brown, William G Davis, John D Klena, Pierre E Rollin, Jonas Schmidt-Chanasit, Elisabeth Fichet-Calvet, Bernd Noack, Petra Emmerich, Toni Rieger, Svenja Wolff, Sarah Katharina Fehling, Markus Eickmann, Jan Philipp Mengel, Tilman Schultze, Torsten Hain, William Ampofo, Kofi Bonney, Juliana Naa Dedei Aryeequaye, Bruce Ribner, Jay B Varkey, Aneesh K Mehta, G Marshall Lyon, Gerrit Kann, Philipp De Leuw, Gundolf Schuettfort, Christoph Stephan, Ulrike Wieland, Jochen W U Fries, Matthias Kochanek, Colleen S Kraft, Timo Wolf, Stuart T Nichol, Stephan Becker, Ute Ströher, Stephan Günther
We describe a strain of Lassa virus representing a putative new lineage that was isolated from a cluster of human infections with an epidemiologic link to Togo. This finding extends the known range of Lassa virus to Togo.
March 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29453057/mammarenaviruses-deleted-from-their-z-gene-are-replicative-and-produce-an-infectious-progeny-in-bhk-21-cells
#18
Amélie D Zaza, Cécile H Herbreteau, Christophe N Peyrefitte, Sébastien F Emonet
Mammarenaviruses bud out of infected cells via the recruitment of the endosomal sorting complex required for transport through late domain motifs localized into their Z protein. Here, we demonstrated that mammarenaviruses lacking this protein can be rescued and are replicative, despite a 3-log reduction in virion production, in BHK-21 cells, but not in five other cell lines. Mutations of putative late domain motifs identified into the viral nucleoprotein resulted in the almost complete abolition of infectious virion production by Z-deleted mammarenaviruses...
February 13, 2018: Virology
https://www.readbyqxmd.com/read/29439402/mammarenaviral-infection-is-dependent-on-directional-exposure-to-and-release-from-polarized-intestinal-epithelia
#19
Nikole L Warner, Jenny D Jokinen, Juliane I Beier, Kevin J Sokoloski, Igor S Lukashevich
Mammarenavirusesare single-stranded RNA viruses with a bisegmented ambisense genome. Ingestion has been shown as a natural route of transmission for both Lassa virus (LASV) and Lymphocytic choriomeningitis virus (LCMV). Due to the mechanism of transmission, epithelial tissues are among the first host cells to come in contact with the viruses, and as such they potentially play a role in spread of virus to naïve hosts. The role of the intestinal epithelia during arenavirus infection remains to be uncharacterized...
February 10, 2018: Viruses
https://www.readbyqxmd.com/read/29430345/design-of-peptide-based-epitope-vaccine-and-further-binding-site-scrutiny-led-to-groundswell-in-drug-discovery-against-lassa-virus
#20
Mohammad Uzzal Hossain, Taimur Md Omar, Arafat Rahman Oany, K M Kaderi Kibria, Abu Zaffar Shibly, Md Moniruzzaman, Syed Raju Ali, Md Monirul Islam
Lassa virus (LASV) is responsible for an acute viral hemorrhagic fever known as Lassa fever. Sequence analyses of LASV proteome identified the most immunogenic protein that led to predict both T-cell and B-cell epitopes and further target and binding site depiction could allow novel drug findings for drug discovery field against this virus. To induce both humoral and cell-mediated immunity peptide sequence SSNLYKGVY, conserved region 41-49 amino acids were found as the most potential B-cell and T-cell epitopes, respectively...
February 2018: 3 Biotech
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