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https://www.readbyqxmd.com/read/28643254/gene-targeting-in-rabbits-single-step-generation-of-knock-out-rabbits-by-microinjection-of-crispr-cas9-plasmids
#1
Yoshihiro Kawano, Arata Honda
The development of genome editing technology has allowed gene disruptions to be achieved in various animal species and has been beneficial to many mammals. Gene disruption using pluripotent stem cells is difficult to achieve in rabbits, but thanks to advances in genome editing technology, a number of gene disruptions have been conducted. This paper describes a simple and easy method for carrying out gene disruptions in rabbits using CRISPR/Cas9 in which the gene to be disrupted is marked, the presence or absence of off-target candidates is checked, and a plasmid allowing simultaneous expression of Cas9 and sgRNA is constructed...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28623008/yucca-schidigera-can-promote-rabbit-growth-fecundity-affect-the-release-of-hormones-in-vivo-and-in-vitro-induce-pathological-changes-in-liver-and-reduce-ovarian-resistance-to-benzene
#2
Martina Földešiová, Andrej Baláži, Ľubica Chrastinová, Juraj Pivko, Jan Kotwica, Abdel Halim Harrath, Peter Chrenek, Alexander V Sirotkin
This study evaluated the effect of Yucca schidigera (YS) extract on the physiological, reproductive, and endocrine indexes of New Zealand White rabbit does. Six-week-old rabbit does were fed a standard diet (control group) or a diet enriched with 5 or 20g of Y powder extract per 100-kg feed mixture for 350days. The does were artificially inseminated after induction of superovulation. Weight gain; conception and kindling rate; viability of pups and mothers; histopathological state of liver and muscle; plasma levels of progesterone (P4), oxytocin (OT), and prostaglandin F (PGF); and the release of P4, insulin-like growth factor I (IGF-I), OT, and PGF by isolated ovarian fragments and their response to the addition of benzene were analyzed...
June 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28601158/effect-of-corifollitropin-alfa-supplemented-with-or-without-lh-on-ovarian-stimulation-and-embryo-viability-in-rabbit
#3
María Pilar Viudes-de-Castro, Francisco Marco-Jiménez, José Isaí Cedano-Castro, José Salvador Vicente
There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable embryos per donor, in order to either support genetic improvement programs, ex situ conservation or to optimize other biotechnologies. Over time, the use of this biotechnology has shown variable outcomes as a consequence of several factors, such as the origin of exogenous hormone, posology and the effect of gonadotropins used simultaneously, the donor and the environment...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601157/protein-based-flushing-related-blood-urea-nitrogen-effects-on-ovarian-response-embryo-recovery-and-embryo-quality-in-superovulated-ewes
#4
İrfan Tur, Dursun Ali Dínç, Ahmet Semacan
The present study is the first report that evaluates effects of nutritional effects of flushing with differing diet crude protein ratios on blood urea nitrogen (BUN) levels, related some reproductive parameters and embryo quality in ewe. During mating season, before synchronization protocol ewes were fed on alfalfa hay and additive concentrate feeding as flushing. Intra vaginal FGA containing sponges applied for 12 days for the purpose of synchronization and pFSH was administered by 8 declining doses for the purpose of superovulation...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28591825/corticotrophin-releasing-hormone-and-corticosterone-impair-development-of-preimplantation-embryos-by-inducing-oviductal-cell-apoptosis-via-activating-the-fas-system-an-in-vitro-study
#5
Xiu-Wen Tan, Chang-Li Ji, Liang-Liang Zheng, Jie Zhang, Hong-Jie Yuan, Shuai Gong, Jiang Zhu, Jing-He Tan
STUDY QUESTION: What are the mechanisms by which corticotrophin-releasing hormone (CRH) and corticosterone impair the development of preimplantation embryos in the oviduct. SUMMARY ANSWER: CRH and corticosterone do not affect preimplantation embryos directly, but impair their development indirectly by triggering apoptosis of oviductal epithelial cells (OECs) through activation of the Fas system. WHAT IS KNOWN ALREADY: Studies report that stress impairs embryo development with facilitated secretion of CRH and glucocorticoids...
June 7, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28579598/rabbit-models-for-biomedical-research-revisited-via-genome-editing-approaches
#6
Arata Honda, Atsuo Ogura
Although the laboratory rabbit has long contributed to many paradigmatic studies in biology and medicine, it is often considered to be a "classical animal model" because in the last 30 years, the laboratory mouse has been more often used, thanks to the availability of embryonic stem cells that have allowed the generation of gene knockout (KO) animals. However, recent genome-editing strategies have changed this unrivaled condition; so far, more than 10 mammalian species have been added to the list of KO animals...
June 2, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28575154/effects-of-propylene-glycol-or-elevated-lh-during-follicle-development-on-ovulation-fertilization-and-early-embryo-development
#7
Katherine S Hackbart, Robb W Bender, Paulo D Carvalho, Lais M Vieira, Ana R Dresch, Jerry N Guenther, Hidir Gencoglu, Anibal B Nascimento, Randy D Shaver, Milo C Wiltbank
Seventeen non-lactating Holstein cows were superovulated in a Latin-square designed experiment to determine effects of increased propylene glycol (PROP) and LH during antral follicle development on ovarian function, fertilization, and early embryo quality. PROP was orally drenched every 4 h for 7d to induce hyperinsulinemia and associated metabolic changes. LH concentrations were altered by increasing LH (3-fold) during last 2 d of superovulation. Treatment groups were: 1) Control-oral drenching with water plus low-LH preparation; 2) HLH-water plus high-LH preparation; 3) PROP-drenching with PROP plus low-LH; 4) PROP/HLH-PROP plus high-LH...
May 30, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28566018/autoimmune-abnormality-affects-ovulation-and-oocyte-pick-up-in-mrl-mpj-fas-lpr-lpr-mice
#8
M Hosotani, O Ichii, T Nakamura, S Otsuka-Kanazawa, Y Hosny Ali Elewa, Y Kon
Ovulation and oocyte-pick-up are essential processes in fertilization. Herein, we found associations between autoimmune disease and the aforementioned processes in mice. At three and six months, along with the evaluation of autoimmune disease indices, the ovary, mesosalpinx, and oviducts were histologically examined in C57BL/6, MRL/MpJ, and MRL/MpJ-Fas (lpr/lpr) mice as healthy control, mild and severe models of autoimmune disease, respectively. In superovulated mice, the number of "oocyte cumulus complexes" found in the ampulla was macroscopically counted, and that of "ovulated oocytes" was histologically evaluated, as indicated by ruptured follicles or corpora hemorrhagica in ovaries...
January 1, 2017: Lupus
https://www.readbyqxmd.com/read/28535841/the-epigenetic-effects-of-assisted-reproductive-technologies-ethical-considerations
#9
M-C Roy, C Dupras, V Ravitsky
The use of assisted reproductive technologies (ART) has increased significantly, allowing many coping with infertility to conceive. However, an emerging body of evidence suggests that ART could carry epigenetic risks for those conceived through the use of these technologies. In accordance with the Developmental Origins of Health and Disease hypothesis, ART could increase the risk of developing late-onset diseases through epigenetic mechanisms, as superovulation, fertilization methods and embryo culture could impair the embryo's epigenetic reprogramming...
May 24, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28521851/regulation-of-interleukin-11-expression-in-ovulatory-follicles-of-the-rat-ovary
#10
You-Jee Jang, You-Jee Jang, Jae-Il Park, Jae-Il Park, Seong-Eun Jeong, Seong-Eun Jeong, You-Mi Seo, You-Mi Seo, Phuong T M Dam, Phuong T M Dam, Young-Woo Seo, Young-Woo Seo, Bum-Chae Choi, Bum-Chae Choi, Sang-Jin Song, Sang-Jin Song, Sang-Young Chun, Sang-Young Chun, Moon-Kyoung Cho, Moon-Kyoung Cho
The aim of the present study was to examine the regulation of interleukin (IL)-11 expression, as well as the role of IL-11, during ovulation in gonadotropin-primed immature rats. Injection of equine chorionic gonadotropin (eCG), followed by human CG (hCG) to induce superovulation stimulated expression of the Il11 gene in theca cells within 6h, as revealed by northern blot and in situ hybridisation analyses. Real-time reverse transcription-polymerase chain reaction analysis showed that the IL-11 receptor, α subunit gene was expressed in granulosa and theca cells and that injection of hCG had no effect on its expression...
May 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28460674/effect-of-extending-fsh-treatment-on-superovulation-and-embryo-production-in-wood-bison-bison-bison-athabascae
#11
J Manuel Palomino, Miriam P Cervantes, Reuben J Mapletoft, Murray R Woodbury, Gregg P Adams
The effect of extending the length of the FSH treatment protocol on superovulatory response and embryo production was investigated in wood bison during the anovulatory and ovulatory seasons. In Experiment 1 (anovulatory season), follicular wave emergence was synchronized by follicular ablation (Day -1) and bison were assigned randomly to two groups (n = 14/group) and given 200 mg FSH on Day 0 and Day 2 (non-extended group), or 133 mg FSH on Days 0, 2, and 4 (extended group). Human chorionic gonadotropin (hCG; 3000 IU) was given on Day 5 and Day 6 in the non-extended and extended groups, respectively, and bison were inseminated 12 and 24 h later...
June 2017: Theriogenology
https://www.readbyqxmd.com/read/28456001/high-milking-sheep-have-a-lower-ovulation-rate-and-tend-to-yield-fewer-embryos-in-response-to-superovulation-and-intrauterine-artificial-insemination
#12
A Meraï, M Dattena, S Casu, M Rekik, N Lassoued
Antagonistic relationship between milk yield and reproduction is reported in several livestock species. This study aimed to investigate whether genetic merit for milk production in dairy sheep affects responses to superovulation, embryo yield and quality. A total of 21 cross-bred Sarda x Lacaune ewes homogeneous for age, parity and stage of lactation were included. The ewes were stratified as high-producing or low-producing based on their genetic merit for milk production estimated by a pentatrait repeatability animal model...
April 29, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28438565/improvements-in-oocyte-competence-in-superovulated-mice-following-treatment-with-cilostazol-ovulation-of-immature-oocytes-with-high-developmental-rates
#13
Ahmed M Taiyeb, Saeeda A Muhsen-Alanssari, William L Dees, Jill Hiney, Michael E Kjelland, Duane C Kraemer, Mundhir T Ridha-Albarzanchi
Exogenous administration of superovulatory hormones negatively affects oocyte competence in mammals. Phosphodiesterase 3A inhibitors were found to improve competence of oocytes matured in vitro in several species, including humans. This study was therefore designed to define oocyte maturation synchronization and competence, in vivo, using superovulated mice treated with cilostazol, a selective phosphodiesterase 3A inhibitor. Swiss Webster mice were superovulated and treated orally with 7.5mg cilostazol once or twice to result in ovulation of immature oocytes at the metaphase I (MI) or germinal vesicle (GV) stage, respectively...
August 1, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28424461/a-lack-of-coordination-between-sister-chromatids-segregation-and-cytokinesis-in-the-oocytes-of-b6-y-tir-xy-sex-reversed-female-mice
#14
Jia-Qiao Zhu, Seang Lin Tan, Teruko Taketo
The B6.Y(TIR) (XY) mouse develops bilateral ovaries despite the expression of the testis-determining gene Sry during gonadal differentiation. We reported that the oocytes of the XY female are defective in their cytoplasm, resulting in a failure in the second meiotic division after activation or fertilization in vitro. However, the mechanism of meiotic failure or the cause of infertility remained to be clarified. In the present study, we obtained mature oocytes from XY females by superovulation and confirmed that these oocytes also fail in zygotic development...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28419839/near-infrared-laser-irradiation-improves-the-development-of-mouse-pre-implantation-embryos
#15
Masaki Yokoo, Miho Mori
The aim of the present study was to assess the effects of near-infrared laser irradiation on the in vitro development of mouse embryos. Female ICR mice were superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin (hCG), and mated with male mice. Two-cell stage embryos were collected 40 h after administering hCG and cultured in M16 medium. Two-cell embryos (0 h after culture), 8-cell embryos (approx. 30 h after culture), morula (approx. 48 h after culture), and blastocysts (approx...
May 27, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28407855/corrigendum-to-reducing-treatments-in-cattle-superovulation-protocols-by-combining-a-pituitary-extract-with-a-5-hyaluronan-solution-is-it-able-to-diminish-activation-of-the-hypothalamic-pituitary-adrenal-axis-compared-to-the-traditional-protocol-theriogenology
#16
Andrea Biancucci, Tatiana Sbaragli, Antonella Comin, Lakamy Sylla, Maurizio Monaci, Tanja Peric, Giuseppe Stradaioli
No abstract text is available yet for this article.
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407853/effects-of-environmental-enrichment-on-reproductive-performance-and-quantity-and-morphology-of-cumulus-oocyte-complexes-obtained-from-rattus-norvegicus
#17
Joana Fisch, Iáskara Vieira de Oliveira, Juliana Fank, Lia Mara Gomes Paim, Marília Remuzzi Zandoná, Eliana Franco Lopes, Fernanda Bastos de Mello, Alexandre Tavares Duarte de Oliveira
Several researchers have observed that environmental enrichment (EE) can be effective in reducing stressful conditions and abnormal behavior and may provide better reproductive performance in rodents. In this context, this study aimed to evaluate the reproductive performance of Wistar rats reared in three different housing conditions. Animals were separated into breeding pairs, one pair per cage and pairs randomly assigned to three experimental groups (ten couples per group): control group were provided cages without any environmental enrichment; PVC group with PVC pipe; and cardboard roll group with a commercially available cardboard tube...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407512/impact-of-large-ovarian-endometriomas-on-the-response-to-superovulation-for-in-vitro-fertilization-a-retrospective-study
#18
Simone Ferrero, Carolina Scala, Emanuela Tafi, Annalisa Racca, Pier Luigi Venturini, Umberto Leone Roberti Maggiore
OBJECTIVE: To assess the response to superovulation for in vitro fertilization (IVF) in patients with unilateral endometriomas with diameter ≥5cm and in the contralateral healthy ovary. STUDY DESIGN: This retrospective analysis of a prospectively collected database included patients who underwent superovulation for IVF/ICSI cycles and had unoperated single unilateral endometrioma with diameter ≥5cm and healthy contralateral ovary. The primary outcome of the study was to compare the number of oocyte retrieved in the ovary with the endometrioma and the contralateral healthy ovary...
April 2, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28407051/igf1r-expression-in-ovarian-granulosa-cells-is-essential-for-steroidogenesis-follicle-survival-and-fertility-in-female-mice
#19
Sarah C Baumgarten, Marah Armouti, CheMyong Ko, Carlos Stocco
Folliculogenesis is a lengthy process that requires the proliferation and differentiation of granulosa cells (GCs) for preovulatory follicle formation. The most crucial endocrine factor involved in this process is follicle-stimulating hormone (FSH). Interestingly, previous in vitro studies indicated that FSH fails to stimulate GC proliferation in the absence of the insulin-like growth factor 1 receptor (IGF1R). To determine the role of the IGF1R in vivo, female mice with a conditional knockdown of the IGF1R in the GCs were produced and had undetectable levels of IGF1R mRNA and protein in the GCs...
April 12, 2017: Endocrinology
https://www.readbyqxmd.com/read/28397811/a-novel-action-of-follicle-stimulating-hormone-in-the-ovary-promotes-estradiol-production-without-inducing-excessive-follicular-growth-before-puberty
#20
Charlotte M François, Florence Petit, Frank Giton, Alain Gougeon, Célia Ravel, Solange Magre, Joëlle Cohen-Tannoudji, Céline J Guigon
In cyclic females, FSH stimulates ovarian estradiol (E2) production and follicular growth up to the terminal stage. A transient elevation in circulating FSH and E2 levels occurs shortly after birth. But what could be the action of FSH on the ovary during this period, and in particular how it stimulates ovarian steroidogenesis without supporting terminal follicular maturation is intriguing. By experimentally manipulating FSH levels, we demonstrate in mice that the mid-infantile elevation in FSH is mandatory for E2 production by the immature ovary, but that it does not stimulate follicle growth...
April 11, 2017: Scientific Reports
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