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Superovulation

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https://www.readbyqxmd.com/read/29093204/in-vitro-fertilization-in-mice
#1
Robert Taft
In vitro fertilization (IVF) involves fertilization of mature oocytes with capacitated sperm in a tissue culture dish. This technique can generate large numbers of cleavage-stage embryos without using a significant number of single-caged stud males for mating. In addition, sperm penetration is synchronous during incubation with mature oocytes, leading to synchronous development, unlike fertilization in vivo, when natural ovulation is usually spread over time. These embryos can be used for a variety of applications including rapid strain expansion and generation of age-matched experimental cohorts as well as cryopreservation at the two-cell stage (speed cryo)...
November 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29076557/colour-doppler-ultrasound-imaging-as-a-laparoscopy-substitute-to-count-corpora-lutea-in-superovulated-sheep
#2
Phn Pinto, G M Bragança, Mfa Balaro, Ekn Arashiro, G B Dos Santos, G N de Souza, Jmg Souza-Fabjan, J F Da Fonseca, F Z Brandão
This study evaluated colour-Doppler ultrasound imaging (UI) as a substitute for laparoscopy to count the corpora lutea (CL) in superovulated sheep. Twenty-five Santa Ines ewes were superovulated three times at 21-day intervals. Corpora lutea were counted by colour-Doppler UI (CLDOPPLER ) 6 days after each superovulation and confirmed by laparoscopy (CLLAP ) 12 hr later. The mean number of CL was similar for both techniques (2.1 ± 2.5 vs. 2.1 ± 2.7 for CLDOPPLER and CLLAP , respectively) with a significant positive correlation (r = ...
October 27, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29059602/superovulatory-response-following-fsh-ecg-fsh-and-hmg-and-pregnancy-rates-following-transfer-of-hatched-blastocyst-embryos-with-different-diameter-and-shape-in-dromedary-camel
#3
Tahereh Ararooti, Amir Niasari-Naslaji, Behnam Asadi-Moghaddam, Kamal Razavi, Farnaz Panahi
The objectives of this study were to compare three superovulation protocols and to investigate the effect of embryo diameter and shape on pregnancy rates in dromedary camel. In Experiment 1, following follicular wave synchronization of donors using two injections of GnRH (25 μg, im), superovulation was initiated 2 days after the second GnRH (Day 0 of experiment). Then, donors were assigned into three groups (n = 5) and received 1) FSH alone (390 mg), 2) eCG (1000 IU; single dose on Day 0) and FSH (330 mg) and 3) hMG (17...
October 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/29046705/super-pregnancy-in-a-balb-c-mouse-superovulated-with-pmsg
#4
Hanieh Vaseghi, Asghar Mogheiseh, Masood Sepehrimanesh, Mojtaba Kafi, Mohammad Hossein Nooranizadeh
This paper reports a case of super pregnancy in a BALB/c mouse pregnant with 30 pups following induction of superovulation using a PMSG-hCG protocol. Superovulation was induced in 10 mice by injecting 5 IU PMSG followed by 5 IU hCG 48 hours later. Immediately after injection of hCG, animals were placed with males at a ratio of 1 to 1 for 24 hours. On day 14 after mating, animals were killed by cervical dislocation and the uterus was examined for pregnancy and the number of fetuses. The mean (±SEM) number of fetuses observed in ten mice was 5...
September 2017: Laboratory Animal Research
https://www.readbyqxmd.com/read/29039170/-research-progress-on-the-safety-of-offsprings-conceived-by-assisted-reproductive-technology
#5
Liya Wang, Yeqing Qian, Fan Jin
Assisted reproductive technology (ART) employs superovulation, in vitro culture and other micromanipulation to complete oocyte maturation, fertilization and early embryo development. Although these techniques have been successfully applied to solve infertility problems, the process may interfere in cell proliferation, differentiation and growth. The clinical and laboratory studies on the safety issue of ART are reviewed in this article. Studies found that the incidence of birth defects increased in ART offspring...
May 25, 2017: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/29035799/use-of-the-endometriosis-fertility-index-in-daily-practice-a-prospective-evaluation
#6
J Boujenah, I Cedrin-Durnerin, C Herbemont, A Bricou, C Sifer, C Poncelet
OBJECTIVE: To perform a prospective evaluation of postoperative fertility management using the endometriosis fertility index (EFI). STUDY: This prospective non-interventional observational study was performed from January 2013 to February 2016 in a tertiary care university hospital and an assisted reproductive technology (ART) centre. In total, 196 patients underwent laparoscopic surgery for endometriosis-related infertility. Indications for surgery included pelvic pain (dysmenorrhoea, and/or deep dyspareunia), abnormal hysterosalpingogram, and failure to conceive after three or more superovulation cycles with or without intra-uterine insemination...
October 10, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29024972/effect-of-metabolic-status-on-conceptus-maternal-interactions-on-day-19-in-dairy-cattle-ii-effects-on-the-endometrial-transcriptome
#7
Stefan Bauersachs, Constantine A Simintiras, Roger G Sturmey, Stefan Krebs, Jochen Bick, Helmut Blum, Eckhard Wolf, Pat Lonergan, Niamh Forde
The aim of this study was to test the hypothesis that the metabolic stresses associated with lactation alter the ability of the endometrium to respond appropriately to the conceptus by examining endometrial gene expression on day 19 of pregnancy. Immediately after calving, primiparous Holstein cows with similar production and fertility estimated breeding values were randomly divided into two groups and either dried off (i.e. never milked) immediately or milked twice daily. Approximately 65-75 days postpartum, grade 1 blastocysts recovered from superovulated Holstein heifer donors (n = 5) were transferred (1 per recipient) into lactating (n = 11) and nonlactating (n = 11) recipients...
September 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28985546/ovulation-induction-and-controlled-ovarian-stimulation-using-letrozole-gonadotropin-combination-a-single-center-retrospective-cohort-study
#8
Sushila Arya, Sanja Kupesic-Plavsic, Zuber D Mulla, Alok K Dwivedi, Zeni Crisp, Jisha Jose, Luis S Noble
OBJECTIVE: To assess the effect of letrozole in combination with low dose gonadotropins for ovulation induction in anovulatory infertility from polycystic ovary syndrome (PCOS) and controlled ovarian stimulation for endometriosis, and unexplained infertility patients. STUDY DESIGN: Retrospective cohort study in a setting of private Reproductive Endocrinology and Infertility Clinic affiliated with the University. Three hundred couples (650 cycles) requiring OI/COS for PCOS (92 patients, 195 cycles), endometriosis (89 patients, 217 cycles), and unexplained infertility (119 patients, 238 cycles)...
November 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28965278/the-value-of-delaying-hcg-administration-to-enable-maturation-of-medium-sized-follicles-in-patients-undergoing-superovulation-for-ivf-icsi
#9
Awoniyi O Awonuga, Karen Wheeler, Mili Thakur, Roohi Jeelani, Michael P Diamond, Elizabeth E Puscheck
PURPOSE: The purpose of the study is to determine whether continued stimulation of mature follicles to allow "catch up" growth of medium-sized follicles in assisted reproductive technology compromises the clinical pregnancy (CPR) and live birth (LBR) rates in IVF/ICSI cycles. METHODS: This retrospective cohort study reviewed 200 first IVF ± ICSI cycles out of a total of 340 cycles with complete data. Women underwent stimulation protocols with gonadotropins (Gn) and GnRH antagonist...
September 30, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28962017/treatment-with-aicar-inhibits-blastocyst-development-trophectoderm-differentiation-and-tight-junction-formation-and-function-in-mice
#10
Michele D Calder, Nicole A Edwards, Dean H Betts, Andrew J Watson
STUDY QUESTION: What is the impact of adenosine monophosphate-activated protein kinase (AMPK) activation on blastocyst formation, gene expression, and tight junction formation and function? SUMMARY ANSWER: AMPK activity must be tightly controlled for normal preimplantation development and blastocyst formation to occur. WHAT IS KNOWN ALREADY: AMPK isoforms are detectable in oocytes, cumulus cells and preimplantation embryos. Cultured embryos are subject to many stresses that can activate AMPK...
November 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28961951/the-polycystic-ovary-syndrome-associated-gene-yap1-is-regulated-by-gonadotropins-and-sex-steroid-hormones-in-hyperandrogenism-induced-oligo-ovulation-in-mouse
#11
Shu-Yan Ji, Xiao-Man Liu, Bo-Tai Li, Yin-Li Zhang, Hong-Bin Liu, Yu-Chao Zhang, Zi-Jiang Chen, Junping Liu, Heng-Yu Fan
STUDY QUESTION: What is the physiological function of Yes-associated protein-1 (Yap1), a susceptibility gene for polycystic ovary syndrome (PCOS), in ovarian granulosa cells (GCs)? SUMMARY ANSWER: Physiologically, steroid sex hormones stimulate follicle growth by activating YAP1; however, the preovulatory inhibition of YAP1 activity in GCs is a prerequisite of LH actions. WHAT IS KNOWN ALREADY: PCOS is a common gynecologic and endocrine disease with multiple short and long-term consequences...
October 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28960511/effect-of-oestrous-synchrony-between-embryo-donors-and-recipients-embryo-quality-and-state-on-the-pregnancy-rate-in-beef-cattle
#12
McC Rodrigues, Alm Bonotto, Dav Acosta, A A Boligon, M N Corrêa, C C Brauner
This study aimed to evaluate the effect of oestrous synchrony between donors and recipients and the embryo quality on the pregnancy rate in beef cow recipients. The experiment was performed over two years at an embryo transfer (ET) centre in Southern Brazil. Ninety Aberdeen Angus cows were subjected to superovulation (SOV) protocols, resulting in the recovery of 1,048 transferable embryos. Eleven groups were formed with intervals of 6 hr, from -30 to +30 hr, with respect to recipient versus donor oestrous detection...
September 27, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28931827/altered-methylations-of-h19-snrpn-mest-and-peg3-are-reversible-by-developmental-reprogramming-in-kidney-tissue-of-icsi-derived-mice
#13
Qitao Zhan, Xuchen Qi, Ning Wang, Fang Le, Luna Mao, Xinyun Yang, Mu Yuan, Hangying Lou, Xiangrong Xu, Xijing Chen, Fan Jin
Although the prevalence of Intracytoplasmic sperm injection (ICSI) has increased year by year, there remains concern about the safety of these procedures because of reports of the increased risk for imprinting disorders. Previous research has demonstrated that gonadotropin stimulation contributes to an increased incidence of epimutations in ICSI-derived mice. However, the epimutations in ICSI offspring after removing the effect of gonadotropin stimulation and the possibility that epimutations are reversible by developmental reprogramming has not been investigated...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28928773/mesenchymal-stem-cells-in-restoration-of-fertility-at-experimental-pelvic-inflammatory-disease
#14
Nataliia Volkova, Mariia Yukhta, Anatoliy Goltsev
Inflammatory disorders account for a significant percentage of gynecologic diseases, particularly in women of reproductive age. It is known that stem cells have anti-inflammatory and regenerative properties. Based on this, we investigated the effect of intravenous administration of cryopreserved mesenchymal stem cells (cMSCs) of bone marrow on experimental chronic inflammation of the ovaries. The paper shows that on the 21st day after cMSC therapy, leukocyte infiltration of ovaries was slightly relative to the control group without treatment, and the ratio of developing and atretic follicles in the animals with cMSC injection dramatically increased, while in the control, it still remained on the side of atretic forms...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28890423/liposome-encapsulated-diacyl-glycerol-and-inositol-triphosphate-induced-delayed-oocyte-activation-and-poor-development-of-parthenotes
#15
Ramya Nair, Jyothsna Manikkath, Aswathi R Hegde, Srinivas Mutalik, Guruprasad Kalthur, Satish Kumar Adiga
OBJECTIVE: To explore the ability of diacyl glycerol (DAG) and inositol triphosphate (IP3), two major secondary messengers in the calcium signaling pathway, in activating oocytes. MATERIAL AND METHODS: Oocyte cumulus complex obtained from superovulated Swiss albino mice were incubated in M16 medium with liposome-encapsulated 1,2-Dipalmitoyl-sn-glycerol (LEDAG) and/or IP3 for 3 h. Strontium chloride was used as positive control. The activation potential, ploidy status, and blastocyst rate was calculated...
September 1, 2017: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/28888334/preparation-characterization-and-application-of-long-acting-fsh-analogs-for-assisted-reproduction
#16
David Ben-Menahem
Assisted reproduction technologies are widely used in humans and domestic animals and often include follicle stimulating hormone (FSH) in the protocol. One limitation with most of the available FSH preparations is the relative short half-life in the circulation that dictates multiple daily injections for the desired follicle development and superovulation. The development of bioactive long-acting structurally modified FSH analogs is desirable for human and veterinary use. In addition, optimal preparations and/or formulations are expected to improve the regimen and efficiency of the treatment...
September 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28886590/corpora-lutea-in-superovulated-ewes-fed-different-planes-of-nutrition
#17
A Kraisoon, D A Redmer, C S Bass, C Navanukraw, S T Dorsam, V Valkov, A Reyaz, A T Grazul-Bilska
The corpus luteum (CL) is an ovarian structure which is critical for the maintenance of reproductive cyclicity and pregnancy support. Diet and/or diet components may affect some luteal functions. FSH is widely used to induce multiple follicle development and superovulation. We hypothesized that FSH would affect luteal function in ewes fed different nutritional planes. Therefore, the aim of this study was to determine if FSH-treatment affects (1) ovulation rate; (2) CL weight; (3) cell proliferation; (4) vascularity; (5) expression of endothelial nitric oxide (eNOS) and soluble guanylate cyclase (sGC) proteins; and (6) luteal and serum progesterone (P4) concentration in control (C), overfed (O), and underfed (U) ewes at the early- and mid-luteal phases...
August 12, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28865223/improvement-of-dairy-cow-embryo-yield-with-low-level-laser-irradiation
#18
G Palubinskas, V Žilaitis, R Antanaitis
The goal of this study is to estimate the effects of low-level laser irradiation (LLLI) on the superovulatory response according to the number of corpora lutea (CL), follicles (F) and the embryo yield. In recent years, while searching for new, more efficient and organic methods to improve superovulatory response and embryo yield with respect to the conventional methods, low-level laser irradiation (LLLI) is a more sensitive and less costly technology that can be used to improve animal reproduction, namely, artificial insemination and the embryo production system...
March 1, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28859279/the-superovulated-environment-independent-of-embryo-vitrification-results-in-low-birthweight-in-a-mouse-model
#19
Rachel Weinerman, Teri Ord, Marisa S Bartolomei, Christos Coutifaris, Monica Mainigi
Epidemiological studies suggest that babies born following in vitro fertilization (IVF) and fresh embryo transfer are of lower birthweight than babies born following frozen embryo transfer, although the mechanism responsible for this phenotype is not known. We developed a novel mouse model that isolates the independent effects of embryo freezing and the superovulated environment, which cannot be performed in humans. We transferred blastocysts that had been vitrified and warmed, mixed with with fresh blastocysts, into individual pseudopregnant recipients produced by either natural mating or mating following injection with equine chorionic gonadotropin and human chorionic gonadotropin and hCG (superovulation)...
July 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28837782/production-of-inbred-offspring-by-intracytoplasmic-sperm-injection-of-oocytes-from-juvenile-female-mice
#20
Jie Zhu, Wei Cui, Yan-Feng Dai
The aim of the present study was to determine whether the use of oocytes from juvenile female mice would improve the efficiency of intracytoplasmic sperm injection (ICSI). In the present study, 15 adult and 14 juvenile C57BL6/J female mice were superovulated, with 17.8 oocytes per mouse harvested from adults, significantly lower than the 40.2 harvested from juveniles (P<0.01). Sixty and 233 oocytes were harvested from C57BL/6J adult and juvenile mice respectively, activated in 10mM SrCl2+5μgmL-1 cytochalasin B for 5-6h and cultured in potassium simplex optimisation medium (KSOM) for 3...
August 25, 2017: Reproduction, Fertility, and Development
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