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https://www.readbyqxmd.com/read/29331456/vocalization-as-an-indicator-of-estrus-climax-in-holstein-heifers-during-natural-estrus-and-superovulation
#1
Volker Röttgen, Frank Becker, Armin Tuchscherer, Christine Wrenzycki, Sandra Düpjan, Peter C Schön, Birger Puppe
The reliable detection of estrus is an important scientific and practical challenge in dairy cattle farming. Female vocalization may indicate reproductive status, and preliminary evidence suggests that this information can be used to detect estrus in dairy cattle. The aim of this study was to associate the changes in the vocalization rate of dairy heifers with behavioral estrus indicators as well as test the influence of the type of estrus (natural estrus vs. superovulation-induced estrus). We analyzed 6 predefined estrus-related behavior patterns (standing to be mounted, head-side mounting, active mounting, chin resting, being mounted while not standing, and active sniffing in the anogenital region) and vocalization rates in the peri-estrus period (day of estrus ± 1 d) of 12 German Holstein heifers using audio-visual recordings...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29315373/female-offspring-from-chronic-hyperandrogenemic-dams-exhibit-delayed-puberty-and-impaired-ovarian-reserve
#2
Zhiqiang Wang, Mingjie Shen, Ping Xue, Sara A DiVall, James Segars, Sheng Wu
Female offspring of many species exposed to high doses of androgens in utero experience endocrine dysfunction during adulthood. The phenotype of offspring from females with pre-pregnancy hyperandrogenemia and impaired ovulation, however, has not been examined. We developed a mouse model of hyperandrogenemia by implanting a low dose dihydrotestosterone (DHT) pellet 15 days before conception. The serum DHT levels in dams treated with the pellet were 1.7 to 2 fold higher (p<0.005) than levels in dams without DHT...
January 5, 2018: Endocrinology
https://www.readbyqxmd.com/read/29308626/evaluating-the-effect-of-melatonin-on-has2-and-pgr-expression-as-well-as-cumulus-expansion-and-fertility-potential-in-mice
#3
Maryam Ezzati, Leila Roshangar, Jafar Soleimani Rad, Nahid Karimian
OBJECTIVES: Infertility is a worldwide health problem which affects approximately 15% of sexually active couples. One of the factors influencing the fertility is melatonin. Also, protection of oocytes and embryos from oxidative stress inducing chemicals in the culture medium is important. The aim of the present study was to investigate if melatonin could regulate hyaluronan synthase-2 (HAS2) and Progesterone receptor (PGR) expressions in the cumulus cells of mice oocytes and provide an in vitro fertilization (IVF) approach...
April 2018: Cell Journal
https://www.readbyqxmd.com/read/29295897/administration-of-gonadotropins-for-superovulation-in-mice
#4
Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy
For experiments that require large numbers of preimplantation mouse embryos, such as microinjection of zygotes, gonadotropins are administered to females before mating to increase the number of oocytes that are ovulated (i.e., to induce superovulation). Pregnant mare serum gonadotropin (PMSG) is used to mimic the oocyte maturation effect of the endogenous follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG) is used to mimic the ovulation induction effect of luteinizing hormone (LH).
January 2, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29288978/expression-and-function-of-muc1-in-uterine-tissues-during-early-pregnancy-in-sheep-after-natural-oestrous-or-artificially-induced-oestrous
#5
Xiangguo Wang, Boyang Zhu, Shanhui Xiong, Xihui Sheng, Xiaolong Qi, Qizhen Huang, Chaolei Chen, Yong Guo, Hemin Ni
Mucin 1 (MUC1), a cell surface glycoprotein, is expressed mainly in the endometrial luminal epithelium (LE) and glandular epithelium (GE) of the endometrium in many mammalian species including mice, rats, pigs, sheep, horses and humans during various stages of a menstrual or oestrous cycle, where it plays an important role in embryo implantation and placentation. However, the expression and function of MUC1 in uterine tissues during early pregnancy in sheep after artificially-induced oestrous is not known. Therefore, we investigated the expression and function of MUC1 in the early pregnant and non-pregnant uterine tissues of sheep with natural oestrous or artificially-induced oestrous on days 10, 12, 14, 16 and 18 of the cycle by in situ hybridization, quantitative real-time polymerase chain reaction, immunohistochemical staining and western blotting methods...
December 20, 2017: Theriogenology
https://www.readbyqxmd.com/read/29280527/the-effects-of-superovulation-and-reproductive-aging-on-the-epigenome-of-the-oocyte-and-embryo
#6
REVIEW
Kira Lynn Marshall, Rocío Melissa Rivera
A societal preference of delaying maternal age at first childbirth has increased reliance on assisted reproductive technologies/therapies (ART) to conceive a child. Oocytes that have undergone physiologic aging (≥35 years for humans) are now commonly used for ART, yet evidence is building that suboptimal reproductive environments associated with aging negatively affect oocyte competence and embryo development - although the mechanisms underlying these relationship are not yet well understood. Epigenetic programming of the oocyte occurs during its growth within a follicle, so the ovarian stimulation protocols that administer exogenous hormones, as part of the first step for all ART procedures, may prevent the gamete from establishing an appropriate epigenetic state...
December 27, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29233618/nutritional-impact-on-gene-expression-and-competence-of-oocytes-used-to-support-embryo-development-and-livebirth-by-cloning-procedures-in-goats
#7
C C L Fernandes, L H Aguiar, C E M Calderón, A M Silva, J P M Alves, R Rossetto, L R Bertolini, M Bertolini, D Rondina
Changes in the nutritional plan have been shown to affect oocyte quality, crucial to oocyte donors animals used in cloning. This study aimed to evaluate the impact of diets with increasing nutritional levels (maintenance diet=M; 1.3M; 1.6M; 1.9M) fed to goats for four weeks on follicular fluid composition, gene expression and oocyte competence used to cloning in goats. Donor females were superovulated for the retrieval of matured oocytes and physical measurements reported. After four weeks, groups receiving diets above maintenance increased thickness of subcutaneous adipose tissue and body weight, with higher values in 1...
October 19, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29217815/effects-of-nicotine-and-tocotrienol-rich-fraction-supplementation-on-cytoskeletal-structures-of-murine-pre-implantation-embryos
#8
Nurul Kamsani Hamirah, Yuhaniza Shafinie Kamsani, Nor-Ashikin Mohamed Nor Khan, Sharaniza Ab Rahim, Mohd Hamim Rajikin
BACKGROUND Cytoskeletal structures, in particular actin and tubulin, provide a fundamental framework in all cells, including embryos. The objective of this study was to evaluate the effects of nicotine, which is a source of oxidative stress, and subsequent supplementation with Tocotrienol-rich fraction (TRF) on actin and tubulin of 2- and 8-cell murine embryos. MATERIAL AND METHODS Thirty female Balb/C mice were divided into 4 groups: Group 1 received: subcutaneous (sc) injection of 0.9% NaCl; Group 2 received sc injection of 3...
December 8, 2017: Medical Science Monitor Basic Research
https://www.readbyqxmd.com/read/29208313/pursuit-of-a-method-for-single-administration-of-pfsh-for-superstimulation-in-cattle-what-we-have-learned
#9
Gabriel A Bó, Dragan R Rogan, Reuben J Mapletoft
A single dose protocol of FSH for superstimulation in cattle may improve compliance and superovulatory response. A single subcutaneous (sc) administration of pFSH was efficacious, but response depended on body condition and injection site; the adipose tissue pad behind the shoulder was most efficacious. Inconsistent results in Holsteins were partially overcome by sc administration of 75% of the total pFSH dose behind the shoulder on the first day followed by 25% 48 h later. An alternative would be to combine FSH with polymers that cause it to be released slowly over several days...
September 28, 2017: Theriogenology
https://www.readbyqxmd.com/read/29179787/sperm-derived-factors-enhance-the-in-vitro-developmental-potential-of-haploid-parthenotes
#10
Ramya Nair, Shahin Aboobacker, Srinivas Mutalik, Guruprasad Kalthur, Satish Kumar Adiga
Parthenotes are characterized by poor in vitro developmental potential either due to the ploidy status or the absence of paternal factors. In the present study, we demonstrate the beneficial role of sperm-derived factors (SDF) on the in vitro development of mouse parthenotes. Mature (MII) oocytes collected from superovulated Swiss albino mice were activated using strontium chloride (SrCl2) in the presence or absence of various concentrations of SDF in M16 medium. The presence of SDF in activation medium did not have any significant influence on the activation rate...
November 28, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29175682/expression-of-progesterone-receptor-protein-in-the-ovine-uterus-during-the-estrous-cycle-effects-of-nutrition-arginine-and-fsh
#11
Anna T Grazul-Bilska, Jiratti Thammasiri, Aree Kraisoon, Arshi Reyaz, Casie S Bass, Samantha L Kaminski, Chainarong Navanukraw, Dale A Redmer
To evaluate expression of progesterone receptor (PGR) AB in follicle stimulating hormone (FSH)-treated or non-treated sheep administered with arginine (Arg) or saline (Sal) fed a control (C), excess (O) or restricted (U) diet, uterine tissues were collected at the early, mid and/or late luteal phases. In exp. 1, ewes from each diet were randomly assigned to one of two treatments, Arg or Sal administration three times daily from day 0 of the first estrous cycle until uterine tissue collection. In exp. 2, ewes were injected twice daily with FSH on days 13-15 of the first estrous cycle...
November 20, 2017: Theriogenology
https://www.readbyqxmd.com/read/29175175/the-relationship-between-circulating-concentration-of-amh-and-lh-content-in-the-follicle-stimulating-hormone-fsh-preparations-on-follicular-growth-and-ovulatory-response-to-superovulation-in-water-buffaloes
#12
A K Redhead, N Siew, N Lambie, D Carnarvon, R Ramgattie, M Knights
The relationship between circulating concentration of anti-mullerian hormone (AMH) and the LH content of follicle stimulating hormone (FSH) preparation on follicular growth and ovulatory response in water buffaloes was evaluated. A single blood sample was taken from cows (N=31; age: 9.06±0.98years) to determine systemic AMH. Animals with concentrations higher or lower than 194±30pg/ml were placed into LOW and HIGH AMH groups and were assigned randomly to be superovulated FSH containing either a high (FSHp, HLH) or low (FolltropinV, LLH) LH content...
November 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29173240/overweight-negatively-affects-outcome-of-superovulation-treatment-in-female-mice
#13
Dušan Fabian, Janka Babeľová, Štefan Čikoš, Zuzana Šefčíková
Superovulatory response is characterized by a high degree of variability and unpredictability. The aim of the present experimental study was to examine whether the amount of maternal body fat can influence the efficiency of ovarian stimulation with gonadotropins. Female mice of two body condition types, normal and obese, produced in a standardized two-generation model, were subjected to ovarian stimulation using eCG and hCG followed by natural mating. Produced ova and embryos were recovered on day 1 and day 4 of pregnancy respectively, and several quantitative, qualitative and developmental parameters were evaluated in them...
November 27, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29171142/interleukin-6-regulates-expression-of-fos-and-jun-genes-to-affect-the-development-of-mouse-preimplantation-embryos
#14
Chunhua Yu, Xinyan Zhang, Li Wang, Yinan Liu, Na Li, Min Li, Li Chen, Yingyu Liu, Yuanqing Yao
AIM: We investigated whether recombinant mouse interleukin-6 (IL-6) affects the development of preimplantation embryos and induces the -signal transducers and activators of transcription (JAK-STAT) signaling pathway by binding IL-6 signal transducer (IL-6st) and regulating Fos and Jun gene expression, thereby accounting for the negative effect of superovulation on embryo development. METHODS: We compared rates of blastocyst formation from embryos cultured with different concentrations of IL-6 or/and anti-interleukin 6 receptor antibody (anti-IL-6RAb) in superovulated experimental and normal control groups...
November 24, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29164502/superovulation-alters-dna-methyltransferase-protein-expression-in-mouse-oocytes-and-early-embryos
#15
Fatma Uysal, Saffet Ozturk, Gokhan Akkoyunlu
PURPOSE: DNA methylation is an epigenetic mechanism that plays critical roles during mammalian oocyte and preimplantation embryo development. It is achieved by adding a methyl group to the fifth carbon atom of cytosine residues within cytosine-phosphate-guanine (CpG) and non-CpG dinucleotide sites using DNA methyltransferase (DNMT) enzymes for de novo and maintenance methylation processes. DNMT1, DNMT3A, and DNMT3B play important roles in establishing methylation of developmentally related genes in oocytes and early embryos...
November 22, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29153167/a-100-year-review-reproductive-technologies-in-dairy-science
#16
S G Moore, J F Hasler
Reproductive technology revolutionized dairy production during the past century. Artificial insemination was first successfully applied to cattle in the early 1900s. The next major developments involved semen extenders, invention of the electroejaculator, progeny testing, addition of antibiotics to semen during the 1930s and 1940s, and the major discovery of sperm cryopreservation with glycerol in 1949. The 1950s and 1960s were particularly productive with the development of protocols for the superovulation of cattle with both pregnant mare serum gonadotrophin/equine chorionic gonadotrophin and FSH, the first successful bovine embryo transfer, the discovery of sperm capacitation, the birth of rabbits after in vitro fertilization, and the development of insulated liquid nitrogen tanks...
December 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29093204/in-vitro-fertilization-in-mice
#17
Robert Taft
In vitro fertilization (IVF) involves fertilization of mature oocytes with capacitated sperm in a tissue culture dish. This technique can generate large numbers of cleavage-stage embryos without using a significant number of single-caged stud males for mating. In addition, sperm penetration is synchronous during incubation with mature oocytes, leading to synchronous development, unlike fertilization in vivo, when natural ovulation is usually spread over time. These embryos can be used for a variety of applications including rapid strain expansion and generation of age-matched experimental cohorts as well as cryopreservation at the two-cell stage (speed cryo)...
November 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29076557/colour-doppler-ultrasound-imaging-as-a-laparoscopy-substitute-to-count-corpora-lutea-in-superovulated-sheep
#18
Phn Pinto, G M Bragança, Mfa Balaro, Ekn Arashiro, G B Dos Santos, G N de Souza, Jmg Souza-Fabjan, J F Da Fonseca, F Z Brandão
This study evaluated colour-Doppler ultrasound imaging (UI) as a substitute for laparoscopy to count the corpora lutea (CL) in superovulated sheep. Twenty-five Santa Ines ewes were superovulated three times at 21-day intervals. Corpora lutea were counted by colour-Doppler UI (CLDOPPLER ) 6 days after each superovulation and confirmed by laparoscopy (CLLAP ) 12 hr later. The mean number of CL was similar for both techniques (2.1 ± 2.5 vs. 2.1 ± 2.7 for CLDOPPLER and CLLAP , respectively) with a significant positive correlation (r = ...
October 27, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29059602/superovulatory-response-following-fsh-ecg-fsh-and-hmg-and-pregnancy-rates-following-transfer-of-hatched-blastocyst-embryos-with-different-diameter-and-shape-in-dromedary-camel
#19
Tahereh Ararooti, Amir Niasari-Naslaji, Behnam Asadi-Moghaddam, Kamal Razavi, Farnaz Panahi
The objectives of this study were to compare three superovulation protocols and to investigate the effect of embryo diameter and shape on pregnancy rates in dromedary camel. In Experiment 1, following follicular wave synchronization of donors using two injections of GnRH (25 μg, im), superovulation was initiated 2 days after the second GnRH (Day 0 of experiment). Then, donors were assigned into three groups (n = 5) and received 1) FSH alone (390 mg), 2) eCG (1000 IU; single dose on Day 0) and FSH (330 mg) and 3) hMG (17...
October 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/29046705/super-pregnancy-in-a-balb-c-mouse-superovulated-with-pmsg
#20
Hanieh Vaseghi, Asghar Mogheiseh, Masood Sepehrimanesh, Mojtaba Kafi, Mohammad Hossein Nooranizadeh
This paper reports a case of super pregnancy in a BALB/c mouse pregnant with 30 pups following induction of superovulation using a PMSG-hCG protocol. Superovulation was induced in 10 mice by injecting 5 IU PMSG followed by 5 IU hCG 48 hours later. Immediately after injection of hCG, animals were placed with males at a ratio of 1 to 1 for 24 hours. On day 14 after mating, animals were killed by cervical dislocation and the uterus was examined for pregnancy and the number of fetuses. The mean (±SEM) number of fetuses observed in ten mice was 5...
September 2017: Laboratory Animal Research
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