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Superovulation

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https://www.readbyqxmd.com/read/28327456/relationship-between-plasma-anti-m%C3%A3-llerian-hormone-concentrations-during-the-rearing-period-and-subsequent-embryo-productivity-in-japanese-black-cattle
#1
H Nabenishi, G Kitahara, S Takagi, A Yamazaki, T Osawa
To use plasma anti-Müllerian hormone (AMH) concentrations as early-stage markers for donor cow selection, we investigated the relationship between plasma AMH concentrations in Japanese black heifers and subsequent embryo productivity following superovulation treatment. Plasma AMH and nonesterified fatty acid (NEFA) concentrations in 6 heifers were evaluated once per month from 3 mo before successful AI for primiparity to 3 mo postpartum. Following calving, embryo collection by superovulation treatment was performed at 3- to 4-mo intervals...
February 4, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28288431/non-art-pregnancy-predictive-factors-in-infertile-patients-with-peritoneal-superficial-endometriosis
#2
J Boujenah, I Cedrin-Durnerin, C Herbemont, C Sifer, C Poncelet
OBJECTIVE: To study the predictive factors for non-ART pregnancy in infertile women after laparoscopic diagnosis and surgery for isolated superficial peritoneal endometriosis (SUP). STUDY DESIGN: Retrospective observational study from January-2004 to December-2015 in a tertiary care university hospital and Assisted Reproductive Technology (ART) centre. Infertile women with laparoscopic surgery for SUP (with histologic diagnosis) were included. The surgical treatment was followed by spontaneous fertility or post-operative ovarian stimulation (pOS) using superovulation (gonadotrophins)±Intra Uterine Insemination (IUI)...
March 7, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28239895/ultrastructure-of-cell-organelles-in-pre-implantation-embryos-from-cows-with-different-body-condition-score
#3
L Olexiková, J Pivko, A V Makarevich, E Kubovičová, A Ostró, J Živčák, P Chrenek
Morphology of important cell organelles (mitochondria, lipid droplets, vacuoles, inclusion bodies and apoptotic bodies) in embryos derived from cows with different body condition score (BCS) was analysed by transmission electron microscopy (TEM). Embryos were recovered on 7th day after the insemination by a standard non-surgical flushing of the uterine horns from superovulated Holstein Friesian cows with BCS 2, 3, 4 and 5. Thereafter, the good quality blastocysts were processed for TEM. The electronograms were evaluated by stereological analysis...
February 27, 2017: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/28238490/composition-of-single-step-media-used-for-human-embryo-culture
#4
Dean E Morbeck, Nikola A Baumann, Devin Oglesbee
OBJECTIVE: To determine compositions of commercial single-step culture media and test with a murine model whether differences in composition are biologically relevant. DESIGN: Experimental laboratory study. SETTING: University-based laboratory. ANIMAL(S): Inbred female mice were superovulated and mated with outbred male mice. INTERVENTION(S): Amino acid, organic acid, and ions content were determined for single-step culture media: CSC, Global, G-TL, and 1-Step...
February 23, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28237339/embryo-production-in-heifers-with-low-or-high-dry-matter-intake-submitted-to-superovulation
#5
Marcos R Mollo, Pedro L J Monteiro, Ricardo S Surjus, Aline C Martins, Alexandre F Ramos, Gerson B Mourão, Luiz H D Carrijo, Gláucio Lopes, Rodolfo Rumpf, Milo C Wiltbank, Roberto Sartori
This study investigated the influence of feed intake on superovulatory response and embryo production of Nelore heifers. Pubertal heifers were kept in a feedlot and were submitted to the same diets, but with different levels of feed consumption: High (1.7 M; n = 20) or Low (0.7 M; n = 19) feed intake. Heifers in the 1.7 M treatment consumed 170% (2.6% of body weight [BW] in dry matter) and the 0.7 M heifers ate 70% (1.1% of BW in dry matter) of a maintenance diet. After 7 wk on these diets, heifers were treated with eight decreasing doses of follicle-stimulating hormone (FSH) given every 12 h, totaling 133 mg Folltropin (Folltropin-V; Bioniche Animal Health, Canada) per heifer...
April 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28219466/factors-of-importance-when-selecting-sows-as-embryo-donors
#6
A Nohalez, C A Martinez, J Reixach, M Diaz, J Vila, I Colina, I Parrilla, J L Vazquez, J Roca, M A Gil, H Rodriguez-Martinez, E A Martinez, C Cuello
The improvement in porcine embryo preservation and non-surgical embryo transfer (ET) procedures achieved in recent years represents essential progress for the practical use of ET in the pig industry. This study aimed to evaluate the effects of parity, weaning-to-estrus interval (WEI) and season on reproductive and embryonic parameters at day 6 after insemination of donor sows superovulated after weaning. The selection of donor sows was based on their reproductive history, body condition and parity. The effects of parity at weaning (2 to 3, 4 to 5 or 6 to 7 litters), season (fall, winter and spring), and WEI (estrus within 3 to 4 days), and their interactions on the number of corpus luteum, cysts in sows with cysts, number and quality of viable and transferable embryos, embryo developmental stage and recovery and fertilization rates were evaluated using linear mixed effects models...
February 21, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28213529/case-control-study-on-the-use-of-pituitary-derived-hormones-from-sheep-as-a-potential-risk-factor-for-the-occurrence-of-atypical-scrapie-in-great-britain
#7
E Marier, M Dawson, M Simmons, J Hope, A Ortiz-Peláez
A case-control study was conducted in 2013 to investigate the use of pituitary-derived hormones from sheep as a potential risk factor for the presence of atypical scrapie in Great Britain sheep holdings. One hundred and sixty-five holdings were identified as cases. Two equal sets of controls were selected: no case of scrapie and cases of classical scrapie. A total of 495 holdings were selected for the questionnaire survey, 201 responses were received and 190 (38.3 per cent) were suitable for analysis. The variables 'use-of-heat-synchronisation/superovulation' and 'flock size' were significantly associated with the occurrence of atypical scrapie...
February 17, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28181120/comparison-of-three-superovulation-protocols-with-or-without-gnrh-treatment-at-the-time-of-artificial-insemination-on-ovarian-response-and-embryo-quality-in-thai-native-heifers
#8
Vibuntita Chankitisakul, Jakkhaphan Pitchayapipatkul, Phirawit Chuawongboon, Dumrongrak Rakwongrit, Denpong Sakhong, Wuttigrai Boonkum, Thevin Vongpralub
To optimize the superovulation protocol in Thai native cattle, the present research was designed to (1) compare three different protocols designed to induce superstimulation and (2) study the effect of gonadotropin-releasing hormone (GnRH) administration at insemination time (to induce ovulation) on ovarian follicular activities in terms of the number of large follicles, corpora lutea (CLs) and unovulated follicles, and the number and quality of ova/embryos recovered in Thai native heifers. Initially, the estrous cycles of animals (n = 36) at unknown stages were synchronized by two prostaglandin F2α (PGF2α) injections at an interval of 12 days...
March 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28170286/effects-of-alcohol-on-mitochondrial-functions-of-cumulus-cells-in-mice
#9
Wanlu Xu, Lihui Huo, Jingjing Li, Chunli Xu, Shuang Wang, Yanhong Yang, Chuang Liu, Xiaomin Zheng, Xiuliang Feng, Xingrong Yan
Alcohol is an important compound used in food, agriculture, and medicine. In this study, we investigated the effect of alcohol on oocyte quality in mice by exposing animals for different duration times during an estrous cycle. Cumulus-oocyte complexes were collected from mice after pregnant mare serum gonadotropin- and human chorionic gonadotropin-induced superovulation. Ovulation number, E2 level in serum, and parthenogenetic embryo development in vitro were evaluated. Mitochondrial gene expression, mitochondrial membrane potential, and reactive oxygen species (ROS) levels in the cumulus were also assessed...
February 7, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28166964/toxoplasma-gondii-transmission-by-artificial-insemination-in-sheep-with-experimentally-contaminated-frozen-semen
#10
Angélica Consalter, Andressa F Silva, Edwards Frazão-Teixeira, Luis F Matos, Francisco C R de Oliveira, Juliana S Leite, Franciele B F Silva, Ana M R Ferreira
Toxoplasma gondii is a parasite considered one of the major causes of reproductive problems in sheep. Furthermore, the presence of the agent in ram semen urges the possibility of sexual transmission in this species. The aim of this study was to evaluate if ram's frozen semen spiked with T. gondii tachyzoites would be able to cause infection in sheep by laparoscopic artificial insemination (AI). Nine ewes tested seronegative to anti-T. gondii antibodies by the modified agglutination test (MAT) were superovulated and inseminated to collect embryos...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28131573/evaluation-of-genetic-components-in-traits-related-to-superovulation-in-vitro-fertilization-and-embryo-transfer-in-holstein-cattle
#11
K L Parker Gaddis, S Dikmen, D J Null, J B Cole, P J Hansen
The objectives of this study were to estimate variance components and identify regions of the genome associated with traits related to embryo transfer in Holsteins. Reproductive technologies are used in the dairy industry to increase the reproductive rate of superior females. A drawback of these methods remains the variability of animal responses to the procedures. If some variability can be explained genetically, selection can be used to improve animal response. Data collected from a Holstein dairy farm in Florida from 2008 to 2015 included 926 superovulation records (number of structures recovered and number of good embryos), 628 in vitro fertilization records (number of oocytes collected, number of cleaved embryos, number of high- and low-quality embryos, and number of transferrable embryos), and 12,089 embryo transfer records (pregnancy success)...
January 25, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28116645/long-term-effects-of-repeated-superovulation-on-the-uterus-and-mammary-gland-in-rhesus-monkeys
#12
Peipei Yan, Jingyi Xu, Yan Zeng, Guoying Dong, Huarong Cao, Meimei Zheng, Hui Zhu
PURPOSE: The aim of this study is to evaluate the effect of repeated controlled ovarian hyperstimulation (COH) on the structure and function of the uterus and mammary gland. METHODS: Three adult female rhesus monkeys were superovulated up to four times, and three spontaneously ovulating monkeys were used as controls. After a 5-year period, the uterus and mammary gland tissue samples were collected for examination of their structure and function. Further, the expression of certain tumor markers was examined to assess the cancer risk for each organ...
January 23, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28115208/activin-a-stimulates-migration-of-the-fallopian-tube-epithelium-an-origin-of-high-grade-serous-ovarian-cancer-through-non-canonical-signaling
#13
Matthew Dean, David A Davis, Joanna E Burdette
Factors that stimulate the migration of fallopian tube epithelial (FTE)-derived high-grade serous ovarian cancer (HGSOC) to the ovary are poorly elucidated. This study characterized the effect of the ovarian hormone, activin A, on normal FTE and HGSOC. Activin A and TGFβ1 induced an epithelial-to-mesenchymal transition in murine oviductal epithelial (MOE) cells, but only activin A increased migration. The migratory effect of activin A was independent of Smad2/3 and required phospho-AKT, phospho-ERK, and Rac1...
April 10, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28099885/oestrus-synchronisation-and-superovulation-alter-the-cervicovaginal-mucus-proteome-of-the-ewe
#14
Jessie W Maddison, Jessica P Rickard, Naomi C Bernecic, Guillaume Tsikis, Clement Soleilhavoup, Valerie Labas, Lucie Combes-Soia, Gregoire Harichaux, Xavier Druart, Tamara Leahy, Simon P de Graaf
Although essential for artificial insemination (AI) and MOET (multiple ovulation and embryo transfer), oestrus synchronisation and superovulation are associated with increased female reproductive tract mucus production and altered sperm transport. The effects of such breeding practices on the ovine cervicovaginal (CV) mucus proteome have not been detailed. The aim of this study was to qualitatively and quantitatively investigate the Merino CV mucus proteome in naturally cycling (NAT) ewes at oestrus and mid-luteal phase, and quantitatively compare CV oestrus mucus proteomes of NAT, progesterone synchronised (P4) and superovulated (SOV) ewes...
January 15, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28070055/superovulatory-responses-in-cynomolgus-monkeys-macaca-fascicularis-depend-on-the-interaction-between-donor-status-and-superovulation-method-used
#15
Ji-Su Kim, Seung-Bin Yoon, Kang-Jin Jeong, Bo-Woong Sim, Seon-A Choi, Sang-Il Lee, Yeung Bae Jin, Bong-Seok Song, Sang-Rae Lee, Sun-Uk Kim, Kyu-Tae Chang
The current study was performed to investigate the effect of oocyte donor status, including age and body weight, on metaphase II (MII) oocyte recovery using two superovulation methods in cynomolgus monkeys. The use of Method A [recombinant gonadotrophin (75 IU/kg, 3×, 3-day intervals) and human chorionic gonadotropin (hCG)] led to great increases in ovary size and the mean number of MII oocytes retrieved in age- and body-weight-dependent manner; in contrast, both the parameters were similar in Method B [recombinant gonadotrophin (60 IU, twice daily, 6 days), recombinant gonadotropin and recombinant human luteinizing hormone (rhLH) (60 IU, twice daily, 3 days), and hCG]...
January 9, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28069900/impact-of-donor-and-recipient-adiposity-on-feto-placental-growth-in-adolescent-sheep
#16
Jacqueline M Wallace, John S Milne, Clare L Adam, Raymond P Aitken
The influence of maternal obesity during oocyte development and its putative interaction with nutrient reserves at conception on pregnancy outcome were examined in an adolescent sheep model. Donor ewes were nutritionally managed to achieve contrasting adiposity (control [CD]/obese [ObD]) for 6 weeks prior to superovulation, and inseminated by a non-obese sire. Morulae from 6 CD and 7 ObD were transferred in singleton into adolescent recipients of identical age but differing adiposity, classified as relatively fat or thin, respectively...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28040122/vitrification-of-mouse-mii-oocytes-developmental-competency-using-paclitaxel
#17
Farzaneh Fesahat, Azita Faramarzi, Arezoo Khoradmehr, Marjan Omidi, Fatemeh Anbari, Mohammad Ali Khalili
OBJECTIVE: Oocyte cryopreservation provides an important alternative for fertility preservation for women who will be treated with cytotoxic drugs. However, it can cause spindle disorganization of microtubules, putting the zygote at risk for aneuploidy. Paclitaxel is known to stabilize the microtubules that constitute the spindle. The aim of this study was to investigate the suitable concentration of paclitaxel for adding to the vitrification media to improve the developmental potential of post-thawed mature oocytes to blastocyst formation in mice...
December 2016: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28017685/physical-exercise-before-pregnancy-helps-the-development-of-mouse-embryos-produced-in-vitro
#18
Wen Hai Xu, Hao Wu, Wei Lan Xia, Hui Lan, Yongsheng Wang, Yong Zhang, Song Hua
Effects of pre-gestational physical activity on the later development of embryos generated in vitro were evaluated. Kunming mice were divided into two groups, namely exercised and unexercised, with the former undergoing physical training on a motor-driven leveled treadmill over a period of 4weeks (5days/week and 60min/day). After that, following superovulation, collection of oocytes from both groups was performed for in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). Notably and specifically, natural mating between the unexercised mice was also done for in vivo fertilization (IVIF)...
December 23, 2016: Mitochondrion
https://www.readbyqxmd.com/read/28002603/association-of-growth-factor-receptor-bound-protein-10-gene-polymorphism-with-superovulation-traits-in-changbaishan-black-cattle
#19
Y Wu, Z Zhang, J B Zhang, Q Deng
The application of assisted reproductive technology in animal production benefits the economy and conservation of biological resources. Single nucleotide polymorphism (SNPs) was used as predictive markers for breeding and reproduction. In the present study, we examined the association between a SNP of the grb10 gene and superovulation traits in cattle. Sequencing results indicated a point mutation and statistical analysis showed a significant association of the mutation with superovulation traits. The high number of embryos collected from the heterozygotes suggested that the mutation in the grb10 gene exerted a significant effect on the number of embryos recovered although the quality was not affected...
December 19, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27926922/high-speed-mouse-backcrossing-through-the-female-germ-line
#20
Erin Grove, Sigrid Eckardt, K John McLaughlin
Transferring mouse mutations into specific mouse strain backgrounds can be critical for appropriate analysis of phenotypic effects of targeted genomic alterations and quantitative trait loci. Speed congenic breeding strategies incorporating marker-assisted selection of progeny with the highest percentage target background as breeders for the next generation can produce congenic strains within approximately 5 generations. When mating selected donor males to target strain females, this may require more than 1 year, with each generation lasting 10 to 11 weeks including 3 weeks of gestation and 7 to 8 weeks until the males reach sexual maturity...
2016: PloS One
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