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https://www.readbyqxmd.com/read/29031224/direct-one-pot-synthesis-of-cinnamaldehyde-immobilized-on-gold-nanoparticles-and-their-antibiofilm-properties
#1
Mohankandhasamy Ramasamy, Jin-Hyung Lee, Jintae Lee
The objective of the present study was to develop a one-pot strategy to synthesis gold nanoparticle complexes using cinnamaldehyde, a potent antibiofilm agent which in its free form, exhibits high volatility and unstable nature. Hence, we developed cinnamaldehyde gold nanoparticles (CGNPs) in a single step to overcome the limitations of free cinnamaldehyde. Furthermore, reduction abilities of cinnamaldehyde under different experimental conditions, that is, varying precursor concentrations of cinnamaldehyde and gold, metal salts, pH, temperature, and light sources, were investigated...
October 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29031223/enhanced-antibacterial-activity-and-biocompatibility-of-zinc-incorporated-organic-inorganic-nanocomposite-coatings-via-electrophoretic-deposition
#2
Pin Huang, Kena Ma, Xinjie Cai, Dan Huang, Xu Yang, Jiabing Ran, Fushi Wang, Tao Jiang
Increased use of reconstruction procedures in orthopedics has improved the life of patients undergoing surgery. However, surgical site infection remains a major challenge. Efforts were made to fabricate antibacterial surfaces with good biocompatibility. This present study aimed to fabricate zinc-incorporated chitosan/gelatin (CS/G) nanocomposite coatings on the titanium substrates via electrophoretic deposition (EPD). Physicochemical characterization confirmed that zinc was successfully deposited in a metallic oxide/salt complex status...
October 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29031106/new-insights-into-resistance-to-colistin-and-third-generation-cephalosporins-of-escherichia-coli-in-poultry-portugal-novel-blactx-m-166-and-blaesac-genes
#3
Vera Manageiro, Lurdes Clemente, Rafael Graça, Ivone Correia, Teresa Albuquerque, Eugénia Ferreira, Manuela Caniça
The increasing incidence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae and Gram negative organisms that has been observed in food animals such as poultry, cattle and pigs, are suggestive that animals, food and environment are potential sources of ESBL-producing bacteria. Hence, the aim of this study was to characterized commensal E. coli obtained from healthy broiler and turkey flocks at slaughter for the presence of penicillinases-, ESBL-, extended-spectrum AmpC (ESAC)-, plasmid-mediated quinolone resistance- and MCR-encoding genes...
October 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29029321/rapid-novel-facile-biosynthesized-silver-nanoparticles-from-bacterial-release-induce-biogenicity-and-concentration-dependent-in-vivo-cytotoxicity-with-embryonic-zebrafish-a-mechanistic-insight
#4
Suresh K Verma, Ealisha Jha, Pritam Kumar Panda, Anurag Mishra, Arun Thirumurugan, Biswadeep Das, S K S Parashar, Mrutyunjay Suar
In this study, rapid one step facile synthesis of silver nanoparticles (AgNP) was done using culture supernatant of two Gram positive (B.thuringiensis, S.aureus) and Gram negative (E.coli, S.typhimurium) bacterial strains and were termed as "BTAgNP", "SAAgNP", "ECAgNP" and "STAgNP" respectively. Synthesized AgNPs were well characterized with the help of different standard techniques like FESEM, DLS, UV-Vis spectroscopy and FTIR. Mechanism of AgNPs synthesis was elucidated using in silico approach. In vivo cytotoxicity of synthesized AgNPs was assessed in embryonic Zebrafish model with the help of uptake, oxidative stress and apoptosis induction experimental assays and the mechanism was investigated through in silico approach at the molecular level...
September 28, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29029067/pharmacodynamics-of-fosfomycin-against-esbl-and-or-carbapenemase-producing-enterobacteriaceae
#5
Fiona Fransen, Kelly Hermans, Maria J B Melchers, Claudia C M Lagarde, Joseph Meletiadis, Johan W Mouton
Background: The increase in antibiotic resistance in Gram-negative bacteria and the limited therapeutic options due to the shortage of new antibiotics have increased the interest of the 'old' antibiotic fosfomycin in the treatment of infections. However, there are contradictory reports on the pharmacodynamics of and emergence of resistance to fosfomycin. Methods: Time-kill assays were performed with 11 ESBL-positive and 3 ESBL-negative strains, exposing the bacteria to 2-fold static concentrations from 0...
October 4, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29028495/non-cytotoxic-effect-of-green-synthesized-silver-nanoparticles-and-its-antibacterial-activity
#6
B Senthil, T Devasena, B Prakash, A Rajasekar
Silver nanoparticles (AgNPs) were green synthesized using ethanolic extract of fenugreek leaves and characterized using UV-Vis spectroscopy, Fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), high resolution transmission electron microscopy HRTEM and energy dispersive X-ray analysis (EDX) techniques. The HRTEM results revealed the formation of highly stable, mono dispersed, spherical shaped AgNPs with the size ranging from 20 to 30nm. The presence of flavonoids and their interaction with the AgNPs were confirmed using FTIR...
October 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29028485/a-study-on-%C3%AE-glucan-binding-protein-%C3%AE-gbp-and-its-involvement-in-phenoloxidase-cascade-in-indian-white-shrimp-fenneropenaeus-indicus
#7
Mahalingam Anjugam, Baskaralingam Vaseeharan, Arokiadhas Iswarya, Muthu Amala, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
The present study reports the purification of novel immune molecule β-1, 3 glucan binding protein from the heamolymph of the Indian white shrimp, Fenneropenaeus indicus (Fiβ-GBP). The purified Fiβ-GBP had 95kDa molecular weight in SDS-PAGE analysis. MALDI-TOF/TOF analysis revealed that the purified Fiβ-GBP showed similarity to various crustacean proteins; 48 and 46% similarity was observed for β-1, 3 glucan binding protein of Chinese white shrimp Fenneropenaeus chinensis and banana shrimp Fenneropenaeus merguiensis, with MOWSE score of 3...
October 10, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29027589/diagnosis-and-treatment-of-severe-neurosurgical-patients-with-pyogenic-ventriculitis-caused-by-gram-negative-bacteria
#8
Feng Shang, Yueqiao Xu, Ning Wang, Weitao Cheng, Wenjin Chen, Wanru Duan
The aim of the study is to explore the experiences in diagnosis and treatment of severe neurosurgical patients with pyogenic ventriculitis caused by gram-negative bacteria (G(-)). Nineteen patients with pyogenic ventriculitis were reviewed for their treatment. The bacterial testing results of cerebrospinal fluid (CSF), the clinical intervention, and the patients' prognosis were evaluated. The bacterial smears of ventricular drainage from all the cases were G(-) bacteria. Head CT and MRI scans confirmed that they were intraventricular empyema...
October 13, 2017: Neurological Sciences
https://www.readbyqxmd.com/read/29027446/-interactions-of-aminoglycosides-with-rnas-and-proteins-via-carbohydrate-microarray
#9
Xueyu Wang, Xiaoli Wang, Beilei Zhang, Jing Hu, Jian Yin
Aminoglycosides are broad-spectrum antibacterials to treat bacterial infections, especially gram-negative bacteria infections. However, aminoglycosides are losing efficacy because of the increase in antibiotic resistance and their inherent toxicity, attracting more interests in developing new aminoglycosides. Several clinically used aminoglycosides are mainly exerted by inhibition of protein synthesis through binding to bacterial rRNA. The bacterial ribosome RNA is the most currently exploited RNA drug target...
October 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29027348/type-vi-secretion-systems-in-plant-associated-bacteria
#10
Patricia Bernal, María A Llamas, Alain Filloux
The type VI secretion system (T6SS) is a bacterial nanomachine used to inject effectors into prokaryotic or eukaryotic cells and is thus involved in both host manipulation and inter-bacterial competition. The T6SS is widespread among Gram-negative bacteria, mostly within the Proteobacterium Phylum. This secretion system is commonly found in commensal and pathogenic plant-associated bacteria. Phylogenetic analysis of phytobacterial T6SS clusters shows that they are distributed in the five main clades previously described (group 1 to 5)...
October 13, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29027347/a-two-step-evolutionary-process-establishes-a-non-native-vitamin-b6-pathway-in-bacillus-subtilis
#11
Jonathan Rosenberg, KahYen C Yeak, Fabian M Commichau
Pyridoxal 5'-phosphate (PLP), the most important form of vitamin B6 serves as a cofactor for many proteins. Two alternative pathways for de novo PLP biosynthesis are known: the short deoxy-xylulose-5-phosphate (DXP)-independent pathway, which is present in the Gram-positive model bacterium Bacillus subtilis and the longer DXP-dependent pathway, which has been intensively studied in the Gram-negative model bacterium Escherichia coli. Previous studies revealed that bacteria contain many promiscuous enzymes causing a so-called "underground metabolism", which can be important for the evolution of novel pathways...
October 13, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29026655/sharing-of-carbapenemase-encoding-plasmids-between-enterobacteriaceae-in-uk-sewage-uncovered-by-minion-sequencing
#12
Catherine Ludden, Sandra Reuter, Kim Judge, Theodore Gouliouris, Beth Blane, Francesc Coll, Plamena Naydenova, Martin Hunt, Alan Tracey, Katie L Hopkins, Nicholas M Brown, Neil Woodford, Julian Parkhill, Sharon J Peacock
Dissemination of carbapenem resistance among pathogenic Gram-negative bacteria is a looming medical emergency. Efficient spread of resistance within and between bacterial species is facilitated by mobile genetic elements. We hypothesized that wastewater contributes to the dissemination of carbapenemase-producing Enterobacteriaceae (CPE), and studied this through a cross-sectional observational study of wastewater in the East of England. We isolated clinically relevant species of CPE in untreated and treated wastewater, confirming that waste treatment does not prevent release of CPE into the environment...
July 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29026621/aeromonas-species-endogenous-endophthalmitis
#13
Laura Ryan, Gareth Higgins, Maeve Doyle
INTRODUCTION: Aeromonas spp. are Gram-negative bacteria classically associated with water sources and a variety of clinical infections in humans. CASE PRESENTATION: A 79-year-old female patient presented with gastroenteritis with associated Aeromonas spp. bloodstream infection. Two days after admission she developed eye symptoms and was diagnosed with endophthalmitis and underwent emergency evisceration and implant. Aeromonas spp. was also recovered from intra-ocular samples...
May 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29025364/pharmacokinetic-pharmacodynamic-parameters-for-treatment-optimization-of-infection-due-to-antibiotic-resistant-bacteria-a-summary-for-practical-purposes-in-children-and-adults
#14
Giulia Gustinetti, Giuliana Cangemi, Roberto Bandettini, Elio Castagnola
In the last years, there has been a tremendous increase in the incidence of bacterial infections due to resistant strains, especially multi-drug resistant Gram-negative bacilli. In Europe, a north to south and a west to east gradient was noticed, with more than one third of the K. pneumonia isolates being resistant to carbapenems in few countries. New antibiotics are lacking and, as a consequence, pharmacokinetic/pharmacodynamic parameters, normalized to pathogen minimal inhibitory concentration, are used with increased frequency to treat infections due to difficult-to-treat pathogens...
October 13, 2017: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29024922/isolation-and-whole-genome-analysis-of-endospore-forming-bacteria-from-heroin
#15
Jörn Kalinowski, Björn Ahrens, Arwa Al-Dilaimi, Anika Winkler, Daniel Wibberg, Uwe Schleenbecker, Christian Rückert, Roman Wölfel, Gregor Grass
Infections caused by endospore-forming bacteria have been associated with severe illness and death among persons who inject drugs. Analysis of the bacteria residing in heroin has thus been biased towards species that affect human health. Similarly, exploration of the bacterial diversity of seized street market heroin correlated with the skin microflora of recreational heroin users insofar as different Staphylococus spp. or typical environmental endospore formers including Bacillus cereus and other Bacilli outside the B...
October 4, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29024556/synthesis-of-metal-nanoparticles-in-metal-phenolic-networks-catalytic-and-antimicrobial-applications-of-coated-textiles
#16
Gyeongwon Yun, Shuaijun Pan, Ting-Yi Wang, Junling Guo, Joseph J Richardson, Frank Caruso
The synthesis of metal nanoparticle (NP)-coated textiles (nanotextiles) is achieved by a dipping process in water without toxic chemicals or complicated synthetic procedures. By taking advantage of the unique nature of tannic acid, metal-phenolic network-coated textiles serve as reducing and stabilizing sites for the generation of metal nanoparticles of controllable size. The textiles can be decorated with various metal nanoparticles, including palladium, silver, or gold, and exhibit properties derived from the presence of the metal nanoparticles, for example, catalytic activity in water (>96% over five cycles using palladium nanoparticles) and antibacterial activity against Gram-negative bacteria (inhibition of Escherichia coli using silver nanoparticles) that outperforms a commercial bandage...
October 12, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29024084/characterization-of-and-lipopolysaccharide-binding-to-the-e-coli-lptc-protein-dimer
#17
Kathryn M Schultz, Candice S Klug
Lipopolysaccharide (LPS, endotoxin) is the major component of the outer leaflet of the outer membrane of Gram-negative bacteria such as Escherichia coli and Salmonella typhimurium. LPS is a large lipid containing several acyl chains as its hydrophobic base and numerous sugars as its hydrophilic core and O-antigen domains, and is an essential element of the organisms' natural defenses in adverse environmental conditions. LptC is one of seven members of the lipopolysaccharide transport (Lpt) protein family that functions to transport LPS from the inner membrane (IM) to the outer leaflet of the outer membrane of the bacterium...
October 12, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29022382/opposing-effect-of-lactobacillus-on-in-vitro-klebsiella-pneumoniae-in-biofilm-and-in-an-in-vivo-intestinal-colonisation-model
#18
R Lagrafeuille, S Miquel, D Balestrino, M Vareille-Delarbre, F Chain, P Langella, C Forestier
Beneficial bacteria represent potential sources of therapy, particularly in the battle against antibiotic-resistant pathogens. The Gram-negative bacillus Klebsiella pneumoniae is not only a paradigm of multi-resistant opportunistic pathogen, but it is also able to colonise the human intestine and displays a high capacity to form biofilm. In this study, the anti-biofilm activity of 140 neutralised Lactobacillus supernatants was assessed against K. pneumoniae. Among the 13 strains whose supernatant significantly impaired biofilm formation, Lactobacillus plantarum CIRM653 was selected because it was also able to impair K...
October 12, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/29022348/modified-mesoporous-silica-nanoparticles-with-dual-synergetic-antibacterial-effect
#19
Marios Michailidis, Ioritz Sorzabal-Bellido, Evanthia Adamidou, Yuri Antonio Diaz-Fernandez, Jenny Louise Aveyard, Reut Wengier, Dmitry O Grigoriev, Rasmita Raval, Yehuda Benayahu, Raechelle D'Sa, Dmitry G Shchukin
Application of mesoporous silica nanoparticles as antifouling/antibacterial carriers is limited and specifically with dual synergetic effect. In the present work, mesoporous silica nanoparticles (MSNs) modified with quaternary ammonium salts (QASs) and loaded with the biocide Parmetol S15 were synthesised as functional fillers for antifouling/antibacterial coatings. From the family of the mesoporous silica nanoparticles, MCM-48 was selected as a carrier due to its cubic pore structure, high surface area and high specific pore volume...
October 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29022146/cupriavidus-malaysiensis-sp-nov-a-novel-poly-3-hydroxybutyrate-co-4-hydroxybutyrate-accumulating-bacterium-isolated-from-the-malaysian-environment
#20
Hema Ramachandran, Nur Asilla Hani Shafie, Kumar Sudesh, Mohamad Noor Azizan, Mohamad Isa Abdul Majid, Al-Ashraf Abdullah Amirul
Bacterial classification on the basis of a polyphasic approach was conducted on three poly(3 hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] accumulating bacterial strains that were isolated from samples collected from Malaysian environments; Kulim Lake, Sg. Pinang river and Sg. Manik paddy field. The Gram-negative, rod-shaped, motile, non-sporulating and non-fermenting bacteria were shown to belong to the genus Cupriavidus of the Betaproteobacteria on the basis of their 16S rRNA gene sequence analyses...
October 11, 2017: Antonie Van Leeuwenhoek
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