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https://www.readbyqxmd.com/read/28437232/epidemiological-physiological-and-molecular-characteristics-of-a-brazilian-collection-of-carbapenem-resistant-acinetobacter-baumannii-and-pseudomonas-aeruginosa
#1
Vanessa Cordeiro Dias, Juliana Alves Resende, André Netto Bastos, Lucas Quinet De Andrade Bastos, Victor Quinet De Andrade Bastos, Ricardo Villela Bastos, Cláudio Galuppo Diniz, Vânia Lúcia Da Silva
Nonfermenting Gram-negative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii are widespread in the environment and are increasingly associated with nosocomial infections, often associated with multidrug-resistance phenotypes. This study aimed to evaluate epidemiological, physiological, and molecular characteristics of carbapenem resistance in P. aeruginosa and A. baumannii. In total, 63 nonreplicated strains (44 A. baumannii and 19 P. aeruginosa) were isolated from hospitalized patients. Antimicrobial resistance patterns, biocide tolerance, oxidative stress, hemolytic activity, and biofilm formation were assessed...
February 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28436320/antibacterial-activity-of-desert-truffles-from-saudi-arabia-against-staphylococcus-aureus-and-pseudomonas-aeruginosa
#2
Domenico Schillaci, Maria Grazia Cusimano, Stella Maria Cascioferro, Vita Di Stefano, Vincenzo Arizza, Marco Chiaramonte, Luigi Inguglia, Abdulhakim Bawadekji, Salvatore Davino, Maria Letizia Gargano, Giuseppe Venturella
Medicinal mushrooms represent an unlimited source of polysaccharides with nutritional, antitumoral, antibacterial, and immune-stimulating properties. Traditional studies of epigeous higher Basidiomycetes have recently been joined by studies of hypogeous fungi and, in particular, of so-called desert truffles. With the aim to obtain novel agents against bacteria of clinical importance, we focused on the edible desert truffle mushrooms Tirmania pinoyi, Terfezia claveryi, and Picoa juniperi as sources of new antimicrobial agents...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28435429/antibacterial-and-cytotoxic-activities-of-naphthoquinone-pigments-from-onosma-visianii-clem
#3
Milena D Vukic, Nenad L Vukovic, Gorica T Djelic, Suzana Lj Popovic, Milan M Zaric, Dejan D Baskic, Gordana B Krstic, Vele V Tesevic, Miroslava M Kacaniova
In this study, the antibacterial and cytotoxic activities of isolated compounds from the roots of Onosma visianii were investigated. By using different chromatographic techniques and appropriate spectroscopic methods, the seven naphthoquinones were described: deoxyshikonin ( 1 ), isobutyrylshikonin ( 2 ), α-methylbutyrylshikonin ( 3 ), acetylshikonin ( 4 ), β-hydroxyisovalerylshikonin ( 5 ), 5,8-O-dimethyl isobutyrylshikonin ( 6 ) and 5,8-O-dimethyl deoxyshikonin ( 7 ). Among the tested compounds, 3 and 4 exhibited the highest antibacterial activities toward all tested bacterial species (MIC50 and MIC90 for gram positive bacteria: 6...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28435260/development-of-gold-nanoparticles-coated-with-silica-containing-the-antibiofilm-drug-cinnamaldehyde-and-their-effects-on-pathogenic-bacteria
#4
Mohankandhasamy Ramasamy, Jin-Hyung Lee, Jintae Lee
Emerging resistance to antibiotics is a mounting worldwide health concern and increases the need for nonantibiotic strategies to combat infectious diseases caused by bacterial pathogens. In this study, the authors used the antibiofilm activity of the naturally occurring antimicrobial cinnamaldehyde (CNMA) conjugated to the surface of gold nanoparticles (GNPs) to deliver CNMA efficiently and eradicate biofilms of Gram-negative organisms (enterohemorrhagic Escherichia coli O157:H7, and Pseudomonas aeruginosa), Gram positive (methicillin-sensitive Staphylococcus aureus organisms, and methicillin-resistant Staphylococcus aureus) bacteria...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28434115/interaction-of-selected-phenylpropenes-with-dipalmitoylphosphatidylcholine-membrane-and-their-relevance-to-antibacterial-activity
#5
Riham Gharib, Amal Najjar, Lizette Auezova, Catherine Charcosset, Hélène Greige-Gerges
The effect of structurally closely related phenylpropenes (PPs), estragole, anethole, eugenol, and isoeugenol, on the fluidity of dipalmitoyl phosphatidyl choline (DPPC) liposome membrane was investigated by DSC, Raman, and fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH). Liposomes were prepared by thin-film hydration method at various DPPC:PP molar ratios. The DPH anisotropy measurements of blank and PP-loaded liposomes were performed at 28, 41, and 50 °C, which correspond, respectively, to gel phase, main transition temperature of DPPC, and liquid phase...
April 22, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28433834/antimicrobial-and-anticancer-photodynamic-activity-of-a-phthalocyanine-photosensitizer-with-n-methyl-morpholiniumethoxy-substituents-in-non-peripheral-positions
#6
Jolanta Dlugaszewska, Wojciech Szczolko, Tomasz Koczorowski, Paulina Skupin-Mrugalska, Anna Teubert, Krystyna Konopka, Malgorzata Kucinska, Marek Murias, Nejat Düzgüneş, Jadwiga Mielcarek, Tomasz Goslinski
Photodynamic therapy involves the use of a photosensitizer that is irradiated with visible light in the presence of oxygen, resulting in the formation of reactive oxygen species. A novel phthalocyanine derivative, the quaternary iodide salt of magnesium(II) phthalocyanine with N-methyl morpholiniumethoxy substituents, was synthesized, and characterized. The techniques used included mass spectrometry (MALDI TOF), UV-vis, NMR spectroscopy, and photocytotoxicity against bacteria, fungi and cancer cells. The phthalocyanine derivative possessed typical characteristics of compounds of the phthalocyanine family but the effect of quaternization was observed on the optical properties, especially in terms of absorption efficiency...
April 8, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28433761/a-glycoproteomic-approach-reveals-that-the-s-layer-glycoprotein-of-lactobacillus-kefiri-cidca-83111-is-o-and-n-glycosylated
#7
Gustavo J Cavallero, Mariano Malamud, Adriana C Casabuono, M de Los Ángeles Serradell, Alicia S Couto
In Gram-positive bacteria, such as lactic acid bacteria, general glycosylation systems have not been documented so far. The aim of this work was to characterize in detail the glycosylation of the S-layer protein of Lactobacillus kefiri CIDCA 83111. A reductive β-elimination treatment followed by anion exchange high performance liquid chromatography analysis was useful to characterize the O-glycosidic structures. MALDI-TOF mass spectrometry analysis confirmed the presence of oligosaccharides bearing from 5 to 8 glucose units carrying galacturonic acid...
April 19, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28432554/epigallocatechin-gallate-protects-beas-2b-cells-from-lipopolysaccharide-induced-apoptosis-through-upregulation-of-gastrin-releasing-peptide
#8
Peethambaran Divya, Bijesh Puthusseri, Denny Joseph Kollareth Manual, Mohammed Azharuddin Savanur
Gastrin-releasing peptide (GRP) plays a major role in the development and maintenance of lung epithelial cells by promoting cell division, whereas its suppression causes growth arrest and apoptosis. The present study shows that human bronchial epithelial BEAS-2B cells challenged with lipopolysaccharide (LPS), an endotoxin from gram-negative bacteria, downregulated GRP expression and induced apoptosis via upregulation of p53 and active caspase-3, signifying the importance of GRP in lung epithelial cell survival...
April 21, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28432541/genome-characteristics-of-the-proteorhodopsin-containing-marine-flavobacterium-polaribacter-dokdonensis-dsw-5
#9
Kiyoung Yoon, Ju Yeon Song, Min-Jung Kwak, Soon-Kyeong Kwon, Jihyun F Kim
Bacteria in the genus Polaribacter, belonging to the family Flavobacteriaceae, are typically isolated from marine environments. Polaribacter dokdonensis DSW-5, the type strain of the species, is a Gram-negative bacterium isolated from the East Sea of Korea. Whole genome shotgun sequencing was performed with the HiSeq 2000 platform and paired-end reads were generated at 188-fold coverage. The sequencing reads were assembled into two contigs with a total length of 3.08 Mb. The genome sequences of DSW-5 contain 2,776 proteincoding sequences and 41 RNA genes...
April 22, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28432320/formation-of-cuo-nano-flowered-surfaces-via-submerged-photo-synthesis-of-crystallites-and-their-antimicrobial-activity
#10
Fumika Nishino, Melbert Jeem, Lihua Zhang, Kazumasa Okamoto, Satoshi Okabe, Seiichi Watanabe
We report the fabrication of flower-like CuO nanostructured surfaces via submerged photo-synthesis of crystallites (SPSC), which requires only UV illumination in neutral water. In this paper, we discuss the reaction mechanism of the photochemical formation of the SPSC-fabricated CuO nanostructures in detail based on surface microstructural analyses and a radiation-chemical consideration with additional gamma-ray irradiation. Since the SPSC method for surface nanostructural fabrication can work at low temperatures at atmospheric pressure without using harmful substances, it is a potential fabrication method for green nanotechnology applications...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28431310/characterization-of-bacterial-communities-of-donkey-milk-by-high-throughput-sequencing
#11
Maria de Los Dolores Soto Del Rio, Alessandra Dalmasso, Tiziana Civera, Maria Teresa Bottero
The interest in donkey milk (DM) is growing because of its functional properties and nutritional value, especially for children with allergies and food intolerances. However, most of the available reports of DM microbiota are based on culture-dependent methods to investigate food safety issues and the presence of lactic acid bacteria (LAB). The aim of this study was to determine the composition of DM bacterial communities using a high-throughput sequencing (HTS) approach. Bulk milk samples from Italian donkey dairy farms from two consecutive years were analysed using the MiSeq Illumina platform...
March 30, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28430942/short-term-responses-and-resistance-of-soil-microbial-community-structure-to-elevated-co2-and-n-addition-in-grassland-mesocosms
#12
Marie Simonin, Naoise Nunan, Juliette M G Bloor, Valérie Pouteau, Audrey Niboyet
Nitrogen (N) addition is known to affect soil microbial communities, but the interactive effects of N addition with other drivers of global change remain unclear. The impacts of multiple global changes on the structure of microbial communities may be mediated by specific microbial groups with different life-history strategies. Here, we investigated the combined effects of elevated CO2 and N addition on soil microbial communities using PLFA profiling in a short-term grassland mesocosm experiment. We also examined the linkages between the relative abundance of r- and K-strategist microorganisms and resistance of the microbial community structure to experimental treatments...
April 18, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28430277/rapid-single-cell-detection-and-identification-of-pathogens-by-using-surface-enhanced-raman-spectroscopy
#13
N E Dina, H Zhou, A Colniţă, N Leopold, T Szoke-Nagy, C Coman, C Haisch
For the successful treatment of infections, real-time analysis and enhanced multiplex capacity, sensitivity and cost-effectiveness of the developed detection method are critical. In this work, surface-enhanced Raman scattering (SERS) was employed with the final aim of identification and discrimination of pathogenic bacteria, based on their detected SERS fingerprint at the single-cell level. Several genera of bacteria that are found in most of the isolated infections in bacteraemia were successfully identified in less than 5 minutes without the use of antibodies or other specific receptors...
April 21, 2017: Analyst
https://www.readbyqxmd.com/read/28429713/gating-of-tonb-dependent-transporters-by-substrate-specific-forced-remodelling
#14
Samuel J Hickman, Rachael E M Cooper, Luca Bellucci, Emanuele Paci, David J Brockwell
Membrane proteins play vital roles in inside-out and outside-in signal transduction by responding to inputs that include mechanical stimuli. Mechanical gating may be mediated by the membrane or by protein(s) but evidence for the latter is scarce. Here we use force spectroscopy, protein engineering and bacterial growth assays to investigate the effects of force on complexes formed between TonB and TonB-dependent transporters (TBDT) from Gram-negative bacteria. We confirm the feasibility of protein-only mediated mechanical gating by demonstrating that the interaction between TonB and BtuB (a TBDT) is sufficiently strong under force to create a channel through the TBDT...
April 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28429192/photoinactivation-effect-of-eosin-methylene-blue-and-chlorophyllin-sodium-copper-against-staphylococcus-aureus-and-escherichia-coli
#15
Cynthia S A Caires, Cassia R B Leal, Carlos A N Ramos, Danielle Bogo, Alessandra R Lima, Eduardo J Arruda, Samuel L Oliveira, Anderson R L Caires, Valter A Nascimento
The use of eosin methylene blue according to Giemsa as photosensitizer is presented for the first time in this paper. The present study evaluated the potential application of chlorophyllin sodium copper salt (CuChlNa) and eosin methylene blue according to Giemsa (EMB) as antimicrobial photosensitizers (aPS) for photodynamic inactivation (PDI) of Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. The experiments were performed using S. aureus stain ATCC 25923 and E. coli ATCC 25922 in which five aPS concentrations (0...
April 20, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28428543/microspectrofluorimetry-to-dissect-the-permeation-of-ceftazidime-in-gram-negative-bacteria
#16
Anas Allam, Laure Maigre, Julia Vergalli, Estelle Dumont, Bertrand Cinquin, Rodolphe Alves de Sousa, Jelena Pajovic, Elizabeth Pinet, Nikaia Smith, Jean-Philippe Herbeuval, Matthieu Réfrégiers, Isabelle Artaud, Jean-Marie Pagès
A main challenge in chemotherapy is to determine the in cellulo parameters modulating the drug concentration required for therapeutic action. It is absolutely urgent to understand membrane permeation and intracellular concentration of antibiotics in clinical isolates: passing the membrane barrier to reach the threshold concentration inside the bacterial periplasm or cytoplasm is the pivotal step of antibacterial activity. Ceftazidime (CAZ) is a key molecule of the combination therapy for treating resistant bacteria...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28427461/inhibitory-activities-of-selected-sudanese-medicinal-plants-on-porphyromonas-gingivalis-and-matrix-metalloproteinase-9-and-isolation-of-bioactive-compounds-from-combretum-hartmannianum-schweinf-bark
#17
Ebtihal Abdalla M Mohieldin, Ali Mahmoud Muddathir, Tohru Mitsunaga
BACKGROUND: Periodontal diseases are one of the major health problems and among the most important preventable global infectious diseases. Porphyromonas gingivalis is an anaerobic Gram-negative bacterium which has been strongly implicated in the etiology of periodontitis. Additionally, matrix metalloproteinases-9 (MMP-9) is an important factor contributing to periodontal tissue destruction by a variety of mechanisms. The purpose of this study was to evaluate the selected Sudanese medicinal plants against P...
April 20, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28426936/cholesterol-assisted-bacterial-cell-surface-engineering-for-photodynamic-inactivation-of-gram-positive-and-gram-negative-bacteria
#18
Hao-Ran Jia, Ya-Xuan Zhu, Zhan Chen, Fu-Gen Wu
Antibacterial photodynamic therapy (PDT) which enables effective killing of regular and multidrug-resistant (MDR) bacteria is a promising treatment modality for bacterial infection. However, because most photosensitizer (PS) molecules fail to strongly interact with the surface of Gram-negative bacteria, this technique is only suitable for treating Gram-positive bacterial infection, which largely hampers its practical applications. Herein, we revealed for the first time that cholesterol could significantly facilitate the hydrophobic binding of PSs to the bacterial surface, achieving the hydrophobic interaction-based bacterial cell surface engineering that could effectively photo-inactivate both Gram-negative and Gram-positive bacteria...
April 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28425464/metabolic-labelling-of-the-carbohydrate-core-in-bacterial-peptidoglycan-and-its-applications
#19
Hai Liang, Kristen E DeMeester, Ching-Wen Hou, Michelle A Parent, Jeffrey L Caplan, Catherine L Grimes
Bacterial cells are surrounded by a polymer known as peptidoglycan (PG), which protects the cell from changes in osmotic pressure and small molecule insults. A component of this material, N-acetyl-muramic acid (NAM), serves as a core structural element for innate immune recognition of PG fragments. We report the synthesis of modifiable NAM carbohydrate derivatives and the installation of these building blocks into the backbone of Gram-positive and Gram-negative bacterial PG utilizing metabolic cell wall recycling and biosynthetic machineries...
April 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28424676/seasonal-fluctuations-of-microbial-aerosol-in-live-poultry-markets-and-the-detection-of-endotoxin
#20
Bo Wu, Kai Meng, Liangmeng Wei, Yumei Cai, Tongjie Chai
Microbial aerosol whose species and concentrations are closely related to human health is ubiquitous. The effect of microbes on human and animal health and production performance is, in many cases, caused by the spreading of air. Samples in this experiment were from a live poultry market (LPM) in Tai'an, China, collected three times a day (8 am, 14 pm, and 20 pm) over three consecutive days each month for 11 months (Original sampling plan was a year, the government due to the environmental protection, the was LPM closed)...
2017: Frontiers in Microbiology
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