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Mesenchymal stem cells

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https://www.readbyqxmd.com/read/29667686/rheological-characterization-of-dynamic-remodeling-of-the-pericellular-region-by-human-mesenchymal-stem-cell-secreted-enzymes-in-well-defined-synthetic-hydrogel-scaffolds
#1
Maryam Daviran, Sarah M Longwill, Jonah F Casella, Kelly M Schultz
Human mesenchymal stem cells (hMSCs) dynamically remodel their microenvironment during basic processes, such as migration and differentiation. Migration requires extracellular matrix invasion, necessitating dynamic cell-material interactions. Understanding these interactions is critical to advancing materials designs that harness and manipulate these processes for applications including wound healing and tissue regeneration. In this work, we encapsulate hMSCs in a cell-degradable poly(ethylene glycol)-peptide hydrogel to determine how cell-secreted enzymes, specifically matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), create unique pericellular microenvironments...
April 18, 2018: Soft Matter
https://www.readbyqxmd.com/read/29667532/human-osteoblast-derived-extracellular-matrix-with-high-homology-to-bone-proteome-is-osteopromotive
#2
Marta Baroncelli, Bram C J van der Eerden, Siddharth Chatterji, Enrique Rull Trinidad, Yik Y Kan, Marijke Koedam, Ingmar A J van Hengel, Rodrigo D A M Alves, Lidy E Fratila-Apachitei, Jeroen A A Demmers, Jeroen van de Peppel, Johannes Ptm van Leeuwen
Efficient osteogenic differentiation of mesenchymal stem cells (MSCs) is crucial to accelerate bone formation. In this context, the use of extracellular matrix (ECM) as natural 3D-framework mimicking in vivo tissue architecture is of interest. The aim of this study was to generate a devitalized human osteogenic MSC-derived ECM and to investigate its impact on MSC osteogenic differentiation to improve MSC properties in bone regeneration. The devitalized ECM significantly enhanced MSC adhesion and proliferation...
April 18, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29667344/concise-review-aggressive-colorectal-cancer-role-of-epithelial-cell-adhesion-molecule-in-cancer-stem-cells-and-epithelial-to-mesenchymal-transition
#3
REVIEW
Maximilian Boesch, Gilbert Spizzo, Andreas Seeber
Colorectal cancer (CRC) is one of the most common malignancies worldwide. In spite of various attempts to ameliorate outcome by escalating treatment, significant improvement is lacking particularly in the adjuvant setting. It has been proposed that cancer stem cells (CSCs) and the epithelial-to-mesenchymal transition (EMT) are at least partially responsible for therapy resistance in CRC. The epithelial cell adhesion molecule (EpCAM) was one of the first CSC antigens to be described. Furthermore, an EpCAM-specific antibody (edrecolomab) has the merit of having launched the era of monoclonal antibody treatment in oncology in the 1990s...
April 17, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29667069/the-versatile-role-of-exosomes-in-cancer-progression-diagnostic-and-therapeutic-implications
#4
REVIEW
Vignesh Sundararajan, Fazlul H Sarkar, Thamil Selvee Ramasamy
BACKGROUND: Recent advances in cancer biology have highlighted the relevance of exosomes and nanovesicles as carriers of genetic and biological messages between cancer cells and their immediate and/or distant environments. It has been found that these molecular cues may play significant roles in cancer progression and metastasis. Cancer cells secrete exosomes containing diverse molecules that can be transferred to recipient cells and/or vice versa to induce a plethora of biological processes, including angiogenesis, metastasis formation, therapeutic resistance, epithelial-mesenchymal transition and epigenetic/stemness (re)programming...
April 17, 2018: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/29667027/neonatal-overfeeding-impairs-differentiation-potential-of-mice-subcutaneous-adipose-mesenchymal-stem-cells
#5
Isabelle Dias, Ísis Salviano, André Mencalha, Simone Nunes de Carvalho, Alessandra Alves Thole, Laís Carvalho, Erika Cortez, Ana Carolina Stumbo
Nutritional changes in the development (intrauterine life and postnatal period) may trigger long-term pathophysiological complications such as obesity and cardiovascular disease. Metabolic programming leads to organs and tissues modifications, including adipose tissue, with increased lipogenesis, production of inflammatory cytokines, and decreased glucose uptake. However, stem cells participation in adipose tissue dysfunctions triggered by overfeeding during lactation has not been elucidated. Therefore, this study was the first to evaluate the effect of metabolic programming on adipose mesenchymal stem cells (ASC) from mice submitted to overfeeding during lactation, using the litter reduction model...
April 17, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29666989/effect-of-nanoheat-stimulation-mediated-by-magnetic-nanocomposite-hydrogel-on-the-osteogenic-differentiation-of-mesenchymal-stem-cells
#6
Zheng Cao, Dan Wang, Yongsan Li, Wensheng Xie, Xing Wang, Lei Tao, Yen Wei, Xiumei Wang, Lingyun Zhao
Hyperthermia has been considered as a promising healing treatment in bone regeneration. We designed a tissue engineering hydrogel based on magnetic nanoparticles to explore the characteristics of hyperthermia for osteogenic regeneration. This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3 O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF)...
April 3, 2018: Science China. Life Sciences
https://www.readbyqxmd.com/read/29666957/conditioned-medium-from-umbilical-cord-mesenchymal-stem-cells-improves-nasal-mucosa-damage-by-radiation
#7
Hong-Gang Duan, Fang Ji, Chun-Quan Zheng, Jing Li, Jing Wang
OBJECTIVES: To explore therapeutic effects of conditioned medium from human umbilical cord mesenchymal stem cells (hUC-MSCs) on nasal mucosa radiation damage both in vivo and in vitro. RESULTS: The mucus cilia clearance time (7 and 30 days), degree of mucosal edema (7, 30, 90 and 180 days), cilia coverage (180 days) of concentrated conditioned medium group improved compared with radiotherapy control group. The proliferation and migration abilities of irradiated and non-irradiated nasal epithelial cells significantly increased after culture in bronchial epithelial cell growth medium (BEGM) containing 10% conditioned medium of hUC-MSCs compared to cells cultured in BEGM alone...
April 17, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29666653/mesenchymal-stem-cells-in-oriented-plga-acecm-composite-scaffolds-enhance-structure-specific-regeneration-of-hyaline-cartilage-in-a-rabbit-model
#8
Weimin Guo, Xifu Zheng, Weiguo Zhang, Mingxue Chen, Zhenyong Wang, Chunxiang Hao, Jingxiang Huang, Zhiguo Yuan, Yu Zhang, Mingjie Wang, Jiang Peng, Aiyuan Wang, Yu Wang, Xiang Sui, Wenjing Xu, Shuyun Liu, Shibi Lu, Quanyi Guo
Articular cartilage lacks a blood supply and nerves. Hence, articular cartilage regeneration remains a major challenge in orthopedics. Decellularized extracellular matrix- (ECM-) based strategies have recently received particular attention. The structure of native cartilage exhibits complex zonal heterogeneity. Specifically, the development of a tissue-engineered scaffold mimicking the aligned structure of native cartilage would be of great utility in terms of cartilage regeneration. Previously, we fabricated oriented PLGA/ACECM (natural, nanofibrous, articular cartilage ECM) composite scaffolds...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29666468/a-novel-endogenous-damage-signal-glycyl-trna-synthetase-activates-multiple-beneficial-functions-of-mesenchymal-stem-cells
#9
Se-Ra Park, Hyun-Jin Kim, Se-Ran Yang, Chan Hum Park, Hwa-Yong Lee, In-Sun Hong
During tissue repair, the injury site releases various bioactive molecules as damage signals to actively recruit stem cells to the damaged region. Despite convincing evidence that mesenchymal stem cells (MSCs) can sense damage signals and promote repair processes, the identity of these signals and how these signals regulate stem cell-mediated tissue repair remain unknown. Glycyl tRNA synthetase (GRS) is a ubiquitously expressed enzyme that catalyzes the first step of protein synthesis in all organisms. In addition to this canonical function, we identified for the first time that GRS is released by damaged tissues or cells in response to various injury signals and may function as a damage signal that activates the proliferative, differentiation, and migratory potential of MSCs, possibly through its identified receptor, cadherin-6 (CDH-6)...
April 17, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29666253/biomaterial-surface-energy-driven-ligand-assembly-strongly-regulates-stem-cell-mechanosensitivity-and-fate-on-very-soft-substrates
#10
Tojo Razafiarison, Claude N Holenstein, Tino Stauber, Milan Jovic, Edward Vertudes, Marko Loparic, Maciej Kawecki, Laetitia Bernard, Unai Silvan, Jess G Snedeker
Although mechanisms of cell-material interaction and cellular mechanotransduction are increasingly understood, the mechanical insensitivity of mesenchymal cells to certain soft amorphous biomaterial substrates has remained largely unexplained. We reveal that surface energy-driven supramolecular ligand assembly can regulate mesenchymal stem cell (MSC) sensing of substrate mechanical compliance and subsequent cell fate. Human MSCs were cultured on collagen-coated hydrophobic polydimethylsiloxane (PDMS) and hydrophilic polyethylene-oxide-PDMS (PEO-PDMS) of a range of stiffnesses...
April 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29666119/depot-specific-characteristics-of-adipose-tissue-derived-stromal-cells-in-thyroid-associated-orbitopathy
#11
Janice Siu Chong Wong, Wai Kit Chu, Benjamin Fuk-Loi Li, Chi-Pui Pang, Kelvin Kam-Lung Chong
BACKGROUND: Thyroid-associated orbitopathy (TAO) causes inflammatory fibroproliferation of periocular connective tissues. We compared adipose tissue-derived stem/stromal cells (ADSCs) from three adipose depots of each patient with TAO on mesenchymal, myofibrogenic, adipogenic properties and associated hyaluronan (HA) synthesis. METHODS: ADSCs were generated from periocular (eyelid, orbital) and subcutaneous (abdominal) adipose tissues of three patients with TAO...
April 17, 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29665934/-effect-of-regulating-ppar%C3%AE-by-mtor-signaling-on-adipogenesis-of-bone-marrow-mesenchymal-stem-cells-from-aplastic-anemia
#12
Fang Wei, Wei-Huan Zhang, Xiu-Lian Zhang, Xiao-Jing Ge, Shu-Qin Zhang
OBJECTIVE: To study the effect and mechanism of mTOR signaling on adipogenesis of bone marrow mesenchymal stem cells(BM-MSCs) from aplastic anemia (AA) patients through regulation of PPARγ. METHODS: BM-MSCs were isolated from 24 newly diagnosed AA patients and 24 healthy controls. The surface antigen expression of BM-MSCs was identified by flow cytometry. The capacity of adipogenic differentiation of BM-MSCs was determined by lipid droplets based on Oil Red O staining and by the expression of FABP4 based on Western blot...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665933/-effect-of-mir-99a-5p-on-the-differential-ability-of-human-bone-marrow-mesenchymal-stem-cells
#13
Hui Xu, Jian Wang, Xu Ye, Jing Ming, Su-Jun Li, Xiang Wang, Cai-Xia He, Xing-Bing Wang
OBJECTIVE: To investigate the effect of microRNA-99a-5p (miR-99a-5p) on differentiation ability of human bone marrow mesenchymal stem cells (BM-MSC). METHODS: BM-MSC was cultured and then transfected with miR-99a-5p mimics or inhibitors. The transfection efficiency was detected by real-time quantitative PCR. The effects of miR-99a-5p on the adipogenic and osteogenic differentiation ability of BM-MSC were detected by differentiation experiment. RESULTS: As compared with the negative control group, the expression of miR-99a-5p was significantly up-regulated after transfection with miR-99a-5p mimics(P<0...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665920/-effect-of-bone-marrow-mesenchymal-stem-cells-in-patients-with-multiple-myelima-on-migration-of-myeloma-cells-in-vitro
#14
Xu-Xia Zhang, Ling-Fang Zhang, Le Liu, Hong-Ling Li
OBJECTIVE: To explore the effect of bone marrow mesenchymal stem cells (MSC) in patients with multiple myeloma(MM) on chemotactic migration of myeloma cells in vitro. METHODS: By in vitro co-culture with diffferent MSC, the myeloma cell U266 was divided into 2 groups: group A in which the U266 cells were co-cultured with normal person MSC (N-MSC) and group B in which the U266 cells were co-cultured with MM-MSC. The expression level of CCR1 in U266 cells, migration rate of U266 cells in Transwell, and the effect of supernantant from co-culture of U266 cells with N-MSC and MM-MSC on the migration in Transwell were compared in condition with or without bortezomib...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665441/nanorod-diameter-modulated-osteogenic-activity-of-hierarchical-micropore-nanorod-patterned-coatings-via-a-wnt-%C3%AE-catenin-pathway
#15
Jianhong Zhou, Lingzhou Zhao, Bo Li, Yong Han
Hierarchical micropore/nanorod-patterned strontium doped hydroxyapatite (Ca9 Sr1 (PO4 )6 (OH)2 , Sr1 -HA) structures (MNRs) with different nanorod diameters of about 30, 70 and 150 nm were coated on titanium, to investigate the effect of nanorod diameter on osteogenesis and the involved mechanism. Compared to micropore/nanogranule-patterned Sr1 -HA coating (MNG), MNRs gave rise to dramatically enhanced in vitro mesenchymal stem cell functions including osteogenic differentiation in the absence of osteogenic supplements and in vivo osseointegration related to the nanorod diameter with about 70 nm displaying the best effects...
April 14, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29665439/hyaluronic-acid-conjugated-nanoparticle-delivery-of-sirna-against-twist-reduces-tumor-burden-and-enhances-sensitivity-to-cisplatin-in-ovarian-cancer
#16
Sophia A Shahin, Ruining Wang, Shirleen I Simargi, Altagracia Contreras, Liliana Parra, Louise Qu, Wei Wen, Thanh Dellinger, Juli Unternaehrer, Fuyuhiko Tamanoi, Jeffrey I Zink, Carlotta A Glackin
TWIST protein is critical to development and is activated in many cancers. TWIST regulates epithelial-mesenchymal transition, and is linked to angiogenesis, metastasis, cancer stem cell phenotype, and drug resistance. The majority of epithelial ovarian cancer (EOC) patients with metastatic disease respond well to first-line chemotherapy but most relapse with disease that is both metastatic and drug resistant, leading to a five-year survival rate under 20%. We are investigating the role of TWIST in mediating these relapses...
April 14, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29665386/enzymatically-crosslinked-and-mechanically-tunable-silk-fibroin-pullulan-hydrogels-for-mesenchymal-stem-cells-delivery
#17
Tao Li, Xiongbo Song, Changmei Weng, Xin Wang, Jiangyi Wu, Li Sun, Xiaoyuan Gong, Wei-Nan Zeng, Liu Yang, Cheng Chen
Hydrogels with good biocompatibility, proper degradation rates, and tissue-matched elasticity are widely used in tissue engineering, regenerative medicine, and drug delivery. In this study, enzymatically crosslinked biocompatible hydrogels were successfully developed using silk fibroin (SF) and pullulan (PL) under physiological conditions in the presence of both horseradish peroxidase (HRP) and hydrogen peroxide (H2 O2 ). A series of properties of the hydrogels including gelation time, equilibrium swelling, enzyme degradation, morphology, rheological property, and compression modulus of SF/PL hydrogels were studied by varying the concentration of PL...
April 14, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29665255/concise-review-rational-use-of-mesenchymal-stem-cells-in-the-treatment-of-ischemic-heart-disease
#18
REVIEW
Michael R Ward, Armin Abadeh, Kim A Connelly
The capacity of stem and progenitor cells to stimulate cardiac regeneration has been studied for almost 20 years, with very promising preclinical data and mixed clinical results. Several cell types have been studied, identified by their cell surface markers, differentiation capacity and their secreted growth factors. Bone marrow derived mesenchymal stem cells (MSCs) have been found to have potent regenerative capacity, through multiple mechanisms, including mesoderm lineage differentiation, immunomodulation, and paracrine stimulation...
April 17, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29665002/three-dimensional-collagenous-niche-and-azacytidine-selectively-promote-time-dependent-cardiomyogenesis-from-human-bone-marrow-derived-msc-spheroids
#19
Jyotsna Joshi, Gautam Mahajan, Chandrasekhar R Kothapalli
Endogenous adult cardiac regenerative machinery is not capable of replacing the lost cells following myocardial infarction, often leading to permanent alterations in structure-function-mechanical properties. Regenerative therapies based on delivering autologous stem cells within an appropriate 3D milieu could meet such demand, by enabling homing and directed differentiation of the transplanted cells into lost specialized cell populations. Since type I collagen is the predominant cardiac tissue matrix protein, we here optimized the 3D niche which could promote time-dependent evolution of cardiomyogenesis from human bone marrow-derived mesenchymal stem cells (BM-MSC)...
April 17, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29663987/-effects-of-hippo-signaling-on-anti-oxidative-stress-of-mesenchymal-stem-cells-in-vitro
#20
Lang Li, Liang Dong, Jiaojie Hui, Fei Gao, Qiuhui Wang, Lan Yang, Jiangqian Zhang, Jie Yan
OBJECTIVE: To explore the effects of Hippo signaling on anti-oxidative stress of mouse marrow mesenchymal stem cells (mMSCs) in vitro. METHODS: mMSCs derived from C57BL/6 mice were identified using fluorescence-activated cell sorting analysis and the capabilities of osteogenic, chondrogenic and adipogenic differentiation were evaluated. 2-deoxy-D-glucose (2-DG) or XMU-MP-1 was used to modulate Hippo signaling. Oxidative stress was induced by H2 O2 treatment and the effect of oxidative stress induced by H2 O2 on survival of mMSCs was evaluated using methyl thiazolyl tetrazolium (MTT) assay...
April 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
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