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Mesenchymal stem cells

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https://www.readbyqxmd.com/read/29036224/immortalized-porcine-mesenchymal-cells-derived-from-nasal-mucosa-lungs-lymph-nodes-spleen-and-bone-marrow-retain-their-stemness-properties-and-trigger-the-expression-of-siglec-1-in-co-cultured-blood-monocytic-cells
#1
Abubakar Garba, Lowiese M B Desmarets, Delphine D Acar, Bert Devriendt, Hans J Nauwynck
Mesenchymal stromal cells have been isolated from different sources. They are multipotent cells capable of differentiating into many different cell types, including osteocytes, chondrocytes and adipocytes. They possess a therapeutic potential in the management of immune disorders and the repair of damaged tissues. Previous work in our laboratory showed an increase of the percentages of CD172a+, CD14+, CD163+, Siglec-1+, CD4+ and CD8+ hematopoietic cells, when co-cultured with immortalized mesenchymal cells derived from bone marrow...
2017: PloS One
https://www.readbyqxmd.com/read/29035932/extracellular-vesicles-in-cartilage-homeostasis-and-osteoarthritis
#2
Shigeru Miyaki, Martin K Lotz
PURPOSE OF REVIEW: Extracellular vesicles carry bioactive molecules that can be transferred between cells and tissues. The purpose of this review is to describe how extracellular vesicles regulate functions of cells in cartilage and other joint tissues. The potential application of extracellular vesicles in the treatment of osteoarthritis and as biomarkers will also be discussed. RECENT FINDINGS: Extracellular vesicles are found in synovial fluid, in articular cartilage and in the supernatants of synoviocytes and chondrocytes...
October 13, 2017: Current Opinion in Rheumatology
https://www.readbyqxmd.com/read/29035736/mechanotransduction-of-human-pluripotent-stem-cells-cultivated-on-tunable-cell-derived-extracellular-matrix
#3
In Gul Kim, Chang-Hyun Gil, Joseph Seo, Soon-Jung Park, Ramesh Subbiah, Taek-Hee Jung, Jong Soo Kim, Young-Hoon Jeong, Hyung-Min Chung, Jong Ho Lee, Man Ryul Lee, Sung-Hwan Moon, Kwideok Park
Cell-derived matrices (CDM) are becoming an attractive alternative to conventional biological scaffolding platforms due to its unique ability to closely recapitulate a native extracellular matrix (ECM) de novo. Although cell-substrate interactions are recognized to be principal in regulating stem cell behavior, very few studies have documented the acclimation of human pluripotent stem cells (hPSCs) on pristine and altered cell-derived matrices. Here, we investigate crosslink-induced mechanotransduction of hPSCs cultivated on decellularized fibroblast-derived matrices (FDM) to explore cell adhesion, growth, migration, and pluripotency in various biological landscapes...
October 9, 2017: Biomaterials
https://www.readbyqxmd.com/read/29035685/mechanistic-effect-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-on-the-submandibular-salivary-gland-in-ovariectomized-rats
#4
Zienab Gouda, Mohamed Khalifa, Sally Shalaby, Samia Hussein
To reveal the effect of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on the submandibular gland after bilateral ovariectomy, 21 adult Sprague-Dawley female rats were divided equally into three groups: sham-operated group (SHAM), ovariectomized group (OVX) and OVX received repeated intravenous injection of hUCB-MSCs group (OVX + hUCB-MSCs). RT-PCR for AQP3, AQP4, AQP5 and BMP6 gene expression was determined. The cellular localization and expression of anti-human CD105 anti-human CD34, anti-proliferating nuclear antigen (PCNA), anti-single stranded DNA(ssDNA), anti-Caspase 3, anti-aquaporin 1(AQP1) and anti-α smooth muscle actin(α-SMA) were determined immunohistochemically...
October 16, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29035380/mixed-cell-therapy-of-bone-marrow-derived-mesenchymal-stem-cells-and-articular-cartilage-chondrocytes-ameliorates-osteoarthritis-development
#5
Indira Prasadam, Akoy Akuien, Thor E Friis, Wei Fang, Xinzhan Mao, Ross W Crawford, Yin Xiao
Of the many cell-based treatments that have been tested in an effort to regenerate osteoarthritic articular cartilage, none have ever produced cartilage that compare with native hyaline cartilage. Studies show that different cell types lead to inconsistent results and for cartilage regeneration to be considered successful, there must be an absence of fibrotic tissue. Here we report of a series of experiments in which bone marrow-derived stem cells (BMSCs) and articular cartilage chondrocytes (ACCs) were mixed in a 1:1 ratio and tested for their ability to enhance cartilage regeneration in three different conditions: (1) in an in vitro differentiation model; (2) in an ex vivo cartilage defect model implanted subcutaneously in mice; and (3) as an intra-articular injection in a meniscectomy-induced OA model in rats...
October 16, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29035367/targeting-glioma-stem-cells-through-combined-bmi1-and-ezh2-inhibition
#6
Xun Jin, Leo J Y Kim, Qiulian Wu, Lisa C Wallace, Briana C Prager, Tanwarat Sanvoranart, Ryan C Gimple, Xiuxing Wang, Stephen C Mack, Tyler E Miller, Ping Huang, Claudia L Valentim, Qi-Gang Zhou, Jill S Barnholtz-Sloan, Shideng Bao, Andrew E Sloan, Jeremy N Rich
Glioblastomas are lethal cancers defined by angiogenesis and pseudopalisading necrosis. Here, we demonstrate that these histological features are associated with distinct transcriptional programs, with vascular regions showing a proneural profile, and hypoxic regions showing a mesenchymal pattern. As these regions harbor glioma stem cells (GSCs), we investigated the epigenetic regulation of these two niches. Proneural, perivascular GSCs activated EZH2, whereas mesenchymal GSCs in hypoxic regions expressed BMI1 protein, which promoted cellular survival under stress due to downregulation of the E3 ligase RNF144A...
October 9, 2017: Nature Medicine
https://www.readbyqxmd.com/read/29035086/notch-signaling-is-essential-for-maturation-self-renewal-and-tri-differentiation-of-in-vitro-derived-human-neural-stem-cells
#7
Katari Venkatesh, L Vinod Kumar Reddy, Salar Abbas, Madhubanti Mullick, Erfath Thanjeem Begum Moghal, Janardhana Papayya Balakrishna, Dwaipayan Sen
Although neural stem cells (NSCs) have potential applications in treating neurological disorders, much still needs to be understood about the differentiation biology for their successful clinical translation. In this study, we aimed at deriving NSCs from human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) and explored the role of Notch signaling in the differentiation process. The hUCB-MSCs were characterized as per guidelines of the International Society of Cellular Therapy. NSCs were successfully generated from hUCB-MSCs by using epidermal and fibroblast growth factors under serum-free conditions...
October 16, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/29034841/ppar-%C3%AE-and-wnt-regulate-the-differentiation-of-mscs-into-adipocytes-and-osteoblasts-respectively
#8
Yue Li, Daxiang Jin, Weixing Xie, Longfei Wen, Weijian Chen, Jixi Xu, Jinyong Ding, Dongcheng Ren
Under the transcriptional control of numerous factors and intracellular signals, mesenchymal stem cells (MSCs) can differentiate into various cell types, including adipocytes and osteoblasts. However, the precise cellular signaling factors that determine the cell fate of MSCs in bone marrow remain largely unknown. Peroxisome proliferator-activated receptor-γ (PPAR-γ) is well established as a prime inducer of adipogenesis, while the Wnt pathway is regarded as the master moderator of osteogenesis. A theoretical inverse relationship exists between adipogenic and osteogenic lineage commitment and differentiation: the differentiation toward an osteoblast phenotype occurs at the expense of an adipocyte phenotype...
October 12, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29033930/immunomodulatory-effects-of-calcium-and-strontium-co-doped-titanium-oxides-on-osteogenesis
#9
Xiangwei Yuan, Huiliang Cao, Jiaxing Wang, Kaiwei Tang, Bin Li, Yaochao Zhao, Mengqi Cheng, Hui Qin, Xuanyong Liu, Xianlong Zhang
The effects of calcium (Ca) or strontium (Sr) on host osteogenesis and immune responses have been investigated separately. In clinical practice, these two elements may both be present around an orthopedic device, but their potential synergistic effects on osteogenesis and the immune response have not been explored to date. In this work, we investigated the immunomodulatory effects of Ca and Sr co-doped titanium oxides on osteogenesis in vitro using the mouse macrophage cell line RAW 264.7 alone and in co-culture with mouse bone mesenchymal stem cells (BMSCs), and in vivo using a mouse air-pouch model...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29033835/farnesol-has-an-anti-obesity-effect-in-high-fat-diet-induced-obese-mice-and-induces-the-development-of-beige-adipocytes-in-human-adipose-tissue-derived-mesenchymal-stem-cells
#10
Hye-Lin Kim, Yunu Jung, Jinbong Park, Dong-Hyun Youn, JongWook Kang, Seona Lim, Beom Su Lee, Mi-Young Jeong, Seong-Kyu Choe, Raekil Park, Kwang Seok Ahn, Jae-Young Um
Brown adipocytes dissipate energy as heat and hence have an important therapeutic capacity for obesity. Development of brown-like adipocytes (also called beige) is also another attractive target for obesity treatment. Here, we investigated the effect of farnesol, an isoprenoid, on adipogenesis in adipocytes and on the browning of white adipose tissue (WAT) as well as on the weight gain of high-fat diet (HFD)-induced obese mice. Farnesol inhibited adipogenesis and the related key regulators including peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α through the up-regulation of AMP-activated protein kinase in 3T3-L1 murine adipocytes and human adipose tissue-derived mesenchymal stem cells (hAMSCs)...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29033380/cd105-is-regulated-by-hsa-mir-1287-and-its-expression-is-inversely-correlated-with-osteopotential-in-shed
#11
Felipe Augusto André Ishiy, Roberto Dalto Fanganiello, Gerson Shigeru Kobayashi, Erika Kague, Patrícia Semedo Kuriki, Maria Rita Passos-Bueno
A more accurate understanding of the molecular mechanisms and signaling pathways underpinning human mesenchymal stem cell (MSC) plasticity and differentiation properties is pivotal for accomplishing solid and diligent translation of MSC-based experimental therapeutics and clinical trials to broad clinical practice. In addition, this knowledge enables selection of MSC subpopulations with increased differentiation potential and/or use of exogenous factors to boost this potential. Here, we report that CD105 (ENG) is a predictive biomarker of osteogenic potential in two types of MSCs: stem cells from human exfoliated deciduous teeth (SHED) and human adipose-derived stem cells (hASC)...
October 12, 2017: Bone
https://www.readbyqxmd.com/read/29033306/oocyte-specific-homeobox-1-obox1-facilitates-reprogramming-by-promoting-mesenchymal-to-epithelial-transition-and-mitigating-cell-hyperproliferation
#12
Li Wu, You Wu, Bing Peng, Zhenzhen Hou, Yu Dong, Kang Chen, Mingyue Guo, Han Li, Xia Chen, Xiaochen Kou, Yanhong Zhao, Yan Bi, Yixuan Wang, Hong Wang, Rongrong Le, Lan Kang, Shaorong Gao
Mammalian oocytes possess fascinating unknown factors, which can reprogram terminally differentiated germ cells or somatic cells into totipotent embryos. Here, we demonstrate that oocyte-specific homeobox 1 (Obox1), an oocyte-specific factor, can markedly enhance the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts in a proliferation-independent manner and can replace Sox2 to achieve pluripotency. Overexpression of Obox1 can greatly promote mesenchymal-to-epithelial transition (MET) at early stage of OSKM-induced reprogramming, and meanwhile, the hyperproliferation of THY1-positive cells can be significantly mitigated...
October 11, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29033030/how-to-use-stem-cells-for-repair-in-stroke-patients
#13
REVIEW
O Detante, C Rome, J Papassin
Regenerative cell therapy is a promising therapeutic strategy in neurology, most notably to improve stroke recovery. Although tolerability and feasibility have apparently been validated, many questions remain as to what is the best type of cells to use, the best route and the post-stroke delay for administration. Two main strategies have currently emerged: intravenous injection of mesenchymal stem cells with systemic trophic support; and intracerebral grafting of neural stem cells with brain repair effects at the lesion site...
October 12, 2017: Revue Neurologique
https://www.readbyqxmd.com/read/29031987/intra-articular-injection-of-mesenchymal-stem-cells-and-platelet-rich-plasma-to-treat-patellofemoral-osteoarthritis-preliminary-results-of-a-long-term-pilot-study
#14
Julien Pintat, Alain Silvestre, Guy Magalon, Alain Pierre Gadeau, Lionel Pesquer, Anne Perozziello, Alain Peuchant, Charbel Mounayer, Benjamin Dallaudière
PURPOSE: To assess the feasibility and safety of concomitant intra-articular (IA) knee injection of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) under fluoroscopic guidance to treat patellofemoral osteoarthritis (OA). MATERIALS AND METHODS: This prospective study included 19 consecutive patients referred for fluoroscopically guided IA MSC and PRP injection for symptomatic patellofemoral chondropathy in which conservative treatment had failed. Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score and magnetic resonance (MR) data, including T2 mapping sequence, were prospectively collected before and 6 months after treatment...
October 12, 2017: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/29031640/the-application-of-mesenchymal-stem-cells-to-treat-thermal-and-radiation-burns
#15
Kathleen Rodgers, Sachin S Jadhav
Mesenchymal stem cells (MSCs) have been developed for a number of indications due to their regenerative and anti-inflammatory phenotypes and their utility is enhanced by the fact that allogeneic transplant is feasible with this cell type. Animal studies and early human cases indicate this has the potential to be an exciting new therapy for treating chronic non-healing wounds such as diabetic ulcers, burns and cutaneous radiation burns. This review will focus on the use of MSCs to treat thermal and radiation burns...
October 11, 2017: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/29031565/quantified-postsurgical-small-cell-size-ctcs-and-epcam-circulating-tumor-stem-cells-with-cytogenetic-abnormalities-in-hepatocellular-carcinoma-patients-determine-cancer-relapse
#16
Liang Wang, Yilin Li, Jing Xu, Aiqun Zhang, Xuedong Wang, Rui Tang, Xinjing Zhang, Hongfang Yin, Manting Liu, Daisy Dandan Wang, Peter Ping Lin, Lin Shen, Jiahong Dong
Detection of hepatocellular carcinoma circulating tumor cells performed with conventional strategies, is significantly limited due to inherently heterogeneous and dynamic expression of EpCAM, as well as degradation of cytokeratins during epithelial-to-mesenchymal transition, which inevitably lead to non-negligible false negative detection of such "uncapturable and invisible" CTCs. A novel SE-iFISH strategy, improved and optimized for detection of HCC-CTCs in this study, was applied to comprehensively detect, phenotypically and karyotypically characterize hepatocellular and cholangiocarcinoma CTCs in patients subjected to surgical resection...
October 12, 2017: Cancer Letters
https://www.readbyqxmd.com/read/29031416/zinc-silicate-mineral-coated-scaffold-improved-in-vitro-osteogenic-differentiation-of-equine-adipose-derived-mesenchymal-stem-cells
#17
Behnaz Bageshlooyafshar, Saeid Vakilian, Mousa Kehtari, Tarlan Eslami-Arshaghi, Farjad Rafeie, Rouhallah Ramezanifard, Reza Rahchamani, Abdollah Mohammadi-Sangcheshmeh, Yousef Mostafaloo, Ehsan Seyedjafari
In current study we aimed to coat the PLLA scaffold with zinc (Zn) silicate mineral nanoparticles. Then, using equine adipose-derived stem cells (ASCs) we intended to compare the osteogenic induction potency of Zn silicate mineral-coated PLLA scaffold with uncoated PLLA scaffold and tissue culture plastic (TCPS). Adipose tissues were collected from 3 horses, and isolation of ASCs was achieved by enzymatic digestion. PLLA scaffold was successfully prepared using a phase separation method and coated with Zn silicate mineral nanoparticles...
September 18, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29031343/musculoskeletal-injuries-and-regenerative-medicine-in-the-elderly-patient
#18
REVIEW
John C Cianca, Prathap Jayaram
Regenerative medicine has gained increasing popularity in its clinical applications, particularly in the field of musculoskeletal medicine. Regenerative medicine, a broad term, can be thought of as a particular medical strategy that strives to rebuild and restore diseased tissue to normal physiologic tissue baseline. Simply put, regenerative strategies augment the body's innate physiology to heal pathologic processes. This article focuses on specific regenerative strategies and the uses of them for common pathologies in the aging adult, including platelet-rich plasma, mesenchymal stem cells, viscosupplementation, and prolotherapy...
November 2017: Physical Medicine and Rehabilitation Clinics of North America
https://www.readbyqxmd.com/read/29031226/ectopic-vascularized-bone-formation-by-human-mesenchymal-stem-cell-microtissues-in-a-biocomposite-scaffold
#19
Rajan Narayan, Tarun Agarwal, Debasish Mishra, Somnath Maji, Sujata Mohanty, Asok Mukhopadhyay, Tapas Kumar Maiti
Three-dimensional multicellular human bone marrow mesenchymal stem cells (hBM-MSCs) are showing a great promise in the repair of bone tissue due to its osteogenic differentiation potential, mimicking in vivo microenvironment and immunomodulatory property. In the present study, the potential of hBM-MSC microtissues (MTs) in combination with a biocomposite material to form vascularized bone-like tissue at an ectopic site in an immunocompromised mouse was evaluated. The scaffold was fabricated using gelatin, carboxymethyl cellulose, polyvinyl alcohol and nano-hydroxyapatite (GCnHP) by the freeze-drying method...
October 5, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29030823/a-facile-in-vitro-384-well-plate-system-to-model-disseminated-tumor-cells-in-the-bone-marrow-microenvironment
#20
Johanna M Buschhaus, Kathryn E Luker, Gary D Luker
Bone marrow disseminated tumor cells (DTCs) are dormant cancer cells that harbor themselves in a bone marrow niche for years after patient remission before potentially returning to a proliferative state, causing recurrent cancer. DTCs reside in bone marrow environments with physiologically important mesenchymal stem cells that are often negatively affected by chemotherapy treatments. Currently, there are very few models of DTCs that recapitulate their dormant phenotype while producing enough samples to accurately quantify cancer and surrounding stromal cell behaviors...
2018: Methods in Molecular Biology
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