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Nordmann poirel

Patrice Nordmann, Alain Mazé, Esther Culebras, Jan Dobias, Aurélie Jayol, Laurent Poirel
The amikacin plus gentamicin-containing SuperAminoglycoside medium was developed for screening multiple-aminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n=59) Gram-negative isolates, including 16S rRNA methylase producers (n=20). Its sensitivity and specificity of detection were, respectively, of 95% and 96% for detecting multiple aminoglycoside-resistant methylase producers...
February 3, 2018: Diagnostic Microbiology and Infectious Disease
Nacim Bouheraoua, Laurent Poirel, Baptiste Bourreau, Rémy Bonnin, Laurent Laroche, Thierry Naas, Patrice Nordmann
Integrons are genetic elements that can acquire and rearrange gene cassettes. The bla BEL-1 gene encodes an extended-spectrum β-lactamase, BEL-1, that is present at second position of the variable region of class 1 integrons identified in Pseudomonas aeruginosa The mobility of the bel-1 gene cassette was analyzed in physiological conditions and with the integrase gene being overexpressed. Cassette mobility in Escherichia coli was detected by excision/integration into the recipient integron In 3 on the conjugative plasmid R388, with the over-produced integrase...
February 26, 2018: Antimicrobial Agents and Chemotherapy
Trestan Pillonel, Patrice Nordmann, Claire Bertelli, Guy Prod' Hom, Laurent Poirel, Gilbert Greub
Carbapenemase-producing Klebsiella pneumoniae are increasingly reported worldwide (1, 2).….
February 26, 2018: Antimicrobial Agents and Chemotherapy
Aurélie Jayol, Patrice Nordmann, Catherine André, Laurent Poirel, Véronique Dubois
Background: The broth microdilution (BMD) method is currently the recommended technique to determine susceptibility to colistin. Objectives: We evaluated the accuracy of three commercialized BMD panels [Sensititre (ThermoFisher Diagnostics), UMIC (Biocentric) and MicroScan (Beckman Coulter)] to determine colistin susceptibility. Methods: A collection of 185 isolates of Gram-negative bacilli (133 colistin resistant and 52 colistin susceptible) was tested...
February 22, 2018: Journal of Antimicrobial Chemotherapy
Daniel Wibberg, Ileana P Salto, Felix G Eikmeyer, Irena Maus, Anika Winkler, Patrice Nordmann, Alfred Pühler, Laurent Poirel, Andreas Schlüter
Multidrug-resistant (MDR) Acinetobacter baumannii strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs). A. baumannii strain K50 recovered from a hospitalized patient in Kuwait exhibited resistance to carbapenems, and additionally to ciprofloxacin, chloramphenicol, sulfonamides, amikacin and gentamicin. Genome sequencing revealed that the strain possesses two plasmids, pK50a (79.6 kb) and pK50b (9.5 kb) and a 3.75 Mb chromosome. A. baumannii K50 exhibits an Average Nucleotide Identity (ANI) value of 99...
February 20, 2018: Antimicrobial Agents and Chemotherapy
Laurent Poirel, Juliette Goutines, Marta Aires-de-Sousa, Patrice Nordmann
Acquired 16S rRNA methylases (RMTase) conferring pandrug resistance to aminoglycosides were searched among enterobacterial isolates recovered in Angola. A total of 36 hospitalized children were screened for rectal colonization using the SuperAminoglycoside medium selective. Twenty-two pan-aminoglycoside-resistant enterobacterial isolates were recovered, all of them producing RMTases, namely RmtB, ArmA, and RmtC. A high diversity of genetic backgrounds was identified either for Escherichia coli and Klebsiella pneumoniae isolates, most of them co-producing carbapenemases NDM-1 or NDM-5, respectively...
February 12, 2018: Antimicrobial Agents and Chemotherapy
Stefano Mancini, Laurent Poirel, Nicolas Kieffer, Patrice Nordmann
PER-1 is an extended-spectrum β-lactamase that is encoded by a gene located into the composite transposon Tn1213 made by two distinct insertion sequences, namely ISPa12 and ISPa13 In vitro mobilization performed in Escherichia coli shows that Tn1213 is functional and is able to mobilize the blaPER-1 gene although at a very low frequency (ca 1x10-9).
January 8, 2018: Antimicrobial Agents and Chemotherapy
Stefano Mancini, Laurent Poirel, Myriam Corthesy, Gilbert Greub, Patrice Nordmann
A carbapenem- and pan-aminoglycoside-resistant Klebsiella pneumoniae strain was isolated from a Brazilian patient hospitalized in a Swiss hospital. The clinical isolate carried genes encoding the KPC-2 carbapenemase and the RmtG 16S rRNA methyltransferase. This is the first report of a carbapenem-resistant K. pneumoniae producing RmtG in Europe.
February 2018: Diagnostic Microbiology and Infectious Disease
Stefano Mancini, Laurent Poirel, Marie-Lise Tritten, Reto Lienhard, Cécile Bassi, Patrice Nordmann
No abstract text is available yet for this article.
November 29, 2017: Journal of Antimicrobial Chemotherapy
Aurélie Jayol, Patrice Nordmann, Laurent Poirel, Véronique Dubois
No abstract text is available yet for this article.
November 18, 2017: Journal of Antimicrobial Chemotherapy
Meritxell Garcia-Quintanilla, Laurent Poirel, Patrice Nordmann
A novel chromogenic medium CHROMagar mSuperCARBA was evaluated to detect carbapenem-resistant Gram-negatives. This medium is as sensitive and as specific as the SUPERCARBA medium for detecting KPC, MBL and OXA-48-type producers (100% and 100%, respectively) and is compatible with subsequent testing of carbapenemase activity using the RAPIDEC(®) CARBA NP.
October 19, 2017: Diagnostic Microbiology and Infectious Disease
Nicolas Kieffer, Marta Aires-de-Sousa, Patrice Nordmann, Laurent Poirel
The mcr-1 (mobile colistin resistance 1) gene, which encodes phosphoethanolamine transferase, has been recently identified as a source of acquired resistance to polymyxins in Escherichia coli. Using the SuperPolymyxin selective medium, we prospectively screened 100 pigs at 2 farms in Portugal for polymyxin-resistant Enterobacteriaceae and recovered 98 plasmid-mediated MCR-1-producing isolates. Most isolates corresponded to nonclonally related E. coli belonging to many sequence types; we also found 2 Klebsiella pneumoniae sequence types...
December 2017: Emerging Infectious Diseases
Laurent Poirel, Yu Larpin, Jan Dobias, Roger Stephan, Jean-Winoc Decousser, Jean-Yves Madec, Patrice Nordmann
The Rapid Polymyxin NP test has been recently developed to rapidly detect polymyxin resistance in Enterobacteriaceae. Here we evaluated this test for detecting MCR-1/MCR-2-producing Enterobacteriaceae using a collection of 70 non-redundant strains either recovered from the environment, animals, or humans. Sensitivity and specificity were found to be 100%.
January 2018: Diagnostic Microbiology and Infectious Disease
Valérie Denervaud-Tendon, Laurent Poirel, Lynn E Connolly, Kevin M Krause, Patrice Nordmann
Objectives: Plazomicin, a novel aminoglycoside with in vitro activity against MDR Gram-negative organisms, is under development to treat patients with serious enterobacterial infections. We evaluated the activity of plazomicin and comparators against colistin-resistant enterobacterial isolates. Methods: Susceptibility to plazomicin and comparators was tested by broth microdilution for a collection of 95 colistin-resistant enterobacterial isolates collected from 29 hospitals in eight countries...
October 1, 2017: Journal of Antimicrobial Chemotherapy
Laurent Poirel, Nicolas Kieffer, José F Fernandez-Garayzabal, Ana Isabel Vela, Yu Larpin, Patrice Nordmann
No abstract text is available yet for this article.
October 1, 2017: Journal of Antimicrobial Chemotherapy
Fernanda Esposito, Miriam R Fernandes, Ralf Lopes, Maria Muñoz, Caetano P Sabino, Marcos P Cunha, Ketrin C Silva, Rodrigo Cayô, Willames M B S Martins, Andrea M Moreno, Terezinha Knöbl, Ana C Gales, Nilton Lincopan
The emergence and rapid dissemination of colistin-resistant Escherichia coli carrying the plasmid-mediated mcr-1 gene have created an urgent need to develop specific screening methods. In this study, we evaluated four assays based on the inhibition of MCR-1 activity by EDTA: (i) a combined-disk test (CDT) comparing the inhibition zones of colistin and colistin (10 μg) plus EDTA (100 mM); (ii) reduction of colistin MIC (CMR) in the presence of EDTA (80 μg/ml); (iii) a modified rapid polymyxin Nordmann/Poirel test (MPNP); and (iv) alteration of zeta potential (RZP = ZP+EDTA /ZP-EDTA )...
December 2017: Journal of Clinical Microbiology
R Deepa, Mythry Ravichandran, S Thasneem Banu, R Sharmila
CONTEXT: Current phenotypic techniques for extended spectrum beta lactamase (ESBL) detection can be interpreted after 24 h of incubation only, resulting in a delay in initiating therapy. Nordmann, Dortet, and Poirel (NDP) in 2012 proposed a novel test named ESBL NDP to overcome this limitation. AIMS: This study aimed to evaluate the ESBL NDP test for the identification of ESBL among Escherichia coli isolates against the Clinical Laboratory Standards Institute-recommended phenotypic confirmatory method...
October 2017: Journal of Laboratory Physicians
Katrin Zurfluh, Magdalena Nüesch-Inderbinen, Jochen Klumpp, Laurent Poirel, Patrice Nordmann, Roger Stephan
BACKGROUND: Mcr-1-harboring Enterobacteriaceae are reported worldwide since their first discovery in 2015. However, a limited number of studies are available that compared full-length plasmid sequences of human and animal origins. METHODS: In this study, mcr-1-bearing plasmids from seven Escherichia coli isolates recovered from patients (n = 3), poultry meat (n = 2) and turkey meat (n = 2) in Switzerland were further analyzed and compared. Isolates were characterized by multilocus sequence typing (MLST)...
2017: Antimicrobial Resistance and Infection Control
Aurélie Jayol, Patrice Nordmann, Adrian Brink, Maria-Virginia Villegas, Véronique Dubois, Laurent Poirel
Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype...
November 2017: Antimicrobial Agents and Chemotherapy
J Dobias, V Dénervaud-Tendon, L Poirel, P Nordmann
The novel siderophore cephalosporin cefiderocol (S-649266) with potent activity against Gram-negative pathogens was recently developed (Shionogi & Co., Ltd.). Here, we evaluated the activity of this new molecule and comparators against a collection of previously characterized Gram-negative isolates using broth microdilution panels. A total of 753 clinical multidrug-resistant Gram-negative isolates collected from hospitals worldwide were tested against cefiderocol and antibiotic comparators (ceftolozane-tazobactam [CT], meropenem [MEM], ceftazidime [CAZ], ceftazidime-avibactam [CZA], colistin [CST], aztreonam [ATM], amikacin [AMK], ciprofloxacin [CIP], cefepime [FEP], and tigecycline [TGC]) for their susceptibility...
December 2017: European Journal of Clinical Microbiology & Infectious Diseases
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