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Aurélie Jayol, Laurent Poirel, Laurent Dortet, Patrice Nordmann
From January 2014 to December 2014, 972 consecutive non-replicate carbapenemase-producing Enterobacteriaceae isolates from colonised or infected patients were collected at the Associated French National Reference Centre as part of the French national survey on antimicrobial resistance. It included 577 Klebsiella spp. (59%), 236 Escherichia coli (24%), 108 Enterobacter spp. (11%), 50 Citrobacter spp. (5%), and a single Salmonella spp. isolate (0.1%). Of 561 K. pneumoniae isolates, 35 were found to be resistant to colistin (6...
September 15, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Cecilia Pozzi, Filomena De Luca, Manuela Benvenuti, Laurent Poirel, Patrice Nordmann, Gian Maria Rossolini, Stefano Mangani, Jean-Denis Docquier
BEL-1 is an acquired class A ESBL found in P. aeruginosa clinical isolates from Belgium, which is divergent from other ESBLs (max. identity, 54% with GES-type enzymes). This enzyme is efficiently inhibited by clavulanate, imipenem and moxalactam. Crystals of BEL-1 were obtained at pH 5.6 and the structure of native BEL-1 was determined from orthorhombic and monoclinic crystal forms, at 1.60-Å and 1.48 Å resolution, respectively. By soaking native BEL-1 crystals, complexes with imipenem (monoclinic form, 1...
September 26, 2016: Antimicrobial Agents and Chemotherapy
Séverine Bontron, Patrice Nordmann, Laurent Poirel
The blaNDM-1 gene encodes a carbapenemase that confers resistance to almost all β-lactams, including latest resort carbapenems. It is increasingly reported worldwide in nosocomial and community-acquired Gram-negative bacteria. Acinetobacter baumannii is an important opportunistic pathogen considered as an intermediate reservoir for the blaNDM-1 gene. In this species, the blaNDM-1 gene is located within the Tn125 composite transposon. The mechanism driving the mobility of Tn125 has not yet been elucidated. Here we experimentally demonstrated transposition of Tn125 in A...
September 26, 2016: Antimicrobial Agents and Chemotherapy
Nadia Liassine, Laetititia Assouvie, Marie-Christine Descombes, Valérie Dénervaud Tendon, Nicolas Kieffer, Laurent Poirel, Patrice Nordmann
No abstract text is available yet for this article.
August 17, 2016: International Journal of Infectious Diseases: IJID
Katrin Zurfluh, Nicolas Kieffer, Laurent Poirel, Patrice Nordmann, Roger Stephan
No abstract text is available yet for this article.
October 2016: Antimicrobial Agents and Chemotherapy
K Zurfluh, D Hindermann, M Nüesch-Inderbinen, L Poirel, P Nordmann, R Stephan
No abstract text is available yet for this article.
June 2016: Schweizer Archiv Für Tierheilkunde
Nicolas Kieffer, Patrice Nordmann, Marta Aires-de-Sousa, Laurent Poirel
This study aimed to evaluate the prevalence of carbapenemase-producing Enterobacteriaceae in Luanda, Angola. A total of 157 rectal samples were collected from children visiting a pediatric hospital in Luanda in March 2015. Fifty-seven imipenem-nonsusceptible enterobacterial isolates were recovered, most of which were non-clonally related. The blaOXA-181 (50/57) and blaNDM-1 (7/57) carbapenemase genes were identified. Notably, OXA-181-producing Escherichia coli isolates rarely coproduced extended-spectrum β-lactamases and consequently remained susceptible to broad-spectrum cephalosporins...
October 2016: Antimicrobial Agents and Chemotherapy
Katrin Zurfluh, Taurai Tasara, Laurent Poirel, Patrice Nordmann, Roger Stephan
We present the draft genome of Escherichia coli S51, a colistin-resistant extended-spectrum β-lactamase-producing strain isolated in 2015 from raw chicken meat imported from Germany. Assembly and annotation of this draft genome resulted in a 4,994,918-bp chromosome and revealed a chromosomally encoded mcr-1 gene responsible for the colistin resistance of the strain.
2016: Genome Announcements
Aurélie Jayol, Véronique Dubois, Laurent Poirel, Patrice Nordmann
Enterobacterial strains resistant to polymyxins are being increasingly reported worldwide. The conventional methods for detection of colistin-resistant isolates such as broth microdilution remain time-consuming (24 to 48 h), and methods such as disc diffusion and Etest are not reliable. Recently, the rapid polymyxin NP test was developed for rapid identification of polymyxin-resistant Enterobacteriaceae This test is based on the detection of glucose metabolism related to bacterial growth in the presence of a defined concentration of colistin (or polymyxin B)...
September 2016: Journal of Clinical Microbiology
Patrice Nordmann, Aurélie Jayol, Laurent Poirel
For identification of polymyxin resistance in Enterobacteriaceae, we developed a rapid test that detects glucose metabolization associated with bacterial growth in the presence of a defined concentration of colistin or polymyxin B. Formation of acid metabolites is evidenced by a color change (orange to yellow) of a pH indicator (red phenol). To evaluate the test, we used bacterial colonies of 135 isolates expressing various mechanisms of colistin resistance (intrinsic, chromosomally encoded, and plasmid-mediated MCR-1) and 65 colistin-susceptible isolates...
June 2016: Emerging Infectious Diseases
Laurent Poirel, Nicolas Kieffer, Adrian Brink, Jennifer Coetze, Aurélie Jayol, Patrice Nordmann
A series of colistin-resistant Escherichia coli clinical isolates was recovered from hospitalized and community patients in South Africa. Seven clonally unrelated isolates harbored the mcr-1 gene located on different plasmid backbones. Two distinct plasmids were fully sequenced, and identical 2,600-bp-long DNA sequences defining a mcr-1 cassette were identified. Promoter sequences responsible for the expression of mcr-1, deduced from the precise identification of the +1 transcription start site for mcr-1, were characterized...
July 2016: Antimicrobial Agents and Chemotherapy
Jennifer Coetzee, Craig Corcoran, Elizabeth Prentice, Mischka Moodley, Marc Mendelson, Laurent Poirel, Patrice Nordmann, Adrian John Brink
The polymyxin antibiotic colistin is an antibiotic of last resort for the treatment of extensively drug-resistant Gram-negative bacteria, including carbapenemase-producing Enterobacteriaceae. The State of the World's Antibiotics report in 2015 highlighted South Africa (SA)'s increasing incidence of these 'superbugs' (3.2% of Klebsiella pneumoniae reported from SA were carbapenemase producers), and in doing so, underscored SA's increasing reliance on colistin as a last line of defence. Colistin resistance effectively renders such increasingly common infections untreatable...
May 2016: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
Séverine Bontron, Laurent Poirel, Patrice Nordmann
OBJECTIVES: The aim of the study was to develop a simple assay for rapid detection of the mcr-1 gene, recently identified as a source of plasmid-mediated acquired resistance to polymyxins in Enterobacteriaceae. METHODS: A SYBR Green-based real-time PCR assay was designed for detection of the mcr-1 gene. This assay was applied to cultured bacteria and to spiked human and cattle stools. RESULTS: The mcr-1 gene could be detected with a lower limit of 10(2) cultured bacteria...
August 2016: Journal of Antimicrobial Chemotherapy
Gülçin Bayramoğlu, Gülşen Uluçam, Çiğdem Gençoğlu Özgür, Ali Osman Kılıç, Faruk Aydın
A rapid, practical, and accurate identification of carbapenemase-producing Enterobacteriaceae isolates is crucial for the implementation of appropriate infection control measures and proper treatment of the infections. For this purpose, a large number of phenotypic test methods have been developed, although none has 100% sensitivity and specificity. Variations in sensitivity and specificity of these tests based on the type of beta-lactamase enzymes carried by that isolates might result in differences between regions and countries...
January 2016: Mikrobiyoloji Bülteni
Laurent Poirel, Patrice Nordmann
No abstract text is available yet for this article.
August 2016: Journal of Antimicrobial Chemotherapy
P Nordmann, L Poirel
No abstract text is available yet for this article.
May 2016: Clinical Microbiology and Infection
Patrice Nordmann, Aurélie Jayol, Laurent Poirel
The colistin-containing SuperPolymyxin medium was developed for screening polymyxin-resistant Gram-negative bacteria. It was evaluated with 88 polymyxin-susceptible or polymyxin-resistant cultured Gram-negative isolates. Its sensitivity and specificity of detection were ca. 100%. The SuperPolymyxin medium is the first screening medium that is able to detect intrinsic and acquired polymyxin-resistant bacteria.
May 2016: Journal of Clinical Microbiology
Thomas Krahn, Daniel Wibberg, Irena Maus, Anika Winkler, Séverine Bontron, Alexander Sczyrba, Patrice Nordmann, Alfred Pühler, Laurent Poirel, Andreas Schlüter
The species Acinetobacter baumannii is one of the most important multidrug-resistant human pathogens. To determine its virulence and antibiotic resistance determinants, the genome of the nosocomial blaNDM-1-positive A. baumannii strain R2090 originating from Egypt was completely sequenced. Genome analysis revealed that strain R2090 is highly related to the community-acquired Australian A. baumannii strain D1279779. The two strains belong to sequence type 267 (ST267). Isolate R2090 harbored the chromosomally integrated transposon Tn125 carrying the carbapenemase gene blaNDM-1 that is not present in the D1279779 genome...
May 2016: Antimicrobial Agents and Chemotherapy
Aurélie Jayol, Patrice Nordmann, Marine Desroches, Jean-Winoc Decousser, Laurent Poirel
An extended-spectrum β-lactamase (ESBL)-producing and colistin-resistant Klebsiella pneumoniae clinical isolate was recovered from a patient who was treated with cefotaxime. This isolate harbored a blaCTX-M-15 ESBL gene that was associated with an ISEcp1 insertion sequence. Transposition of that tandem occurred within the chromosomal mgrB gene, leading to inactivation of the mgrB gene and consequently to acquired resistance to colistin. We showed here a coselection of colistin resistance as a result of a broad-spectrum cephalosporin selective pressure...
May 2016: Antimicrobial Agents and Chemotherapy
Dereje Dadi Gudeta, Valeria Bortolaia, Aurélie Jayol, Laurent Poirel, Patrice Nordmann, Luca Guardabassi
OBJECTIVES: The origin of KPC is unknown. The aim of this study was to detect progenitors of KPC in silico and to functionally verify their β-lactam hydrolysis activity. METHODS: The sequence of KPC-2 was used to mine the NCBI protein sequence database. The best non-KPC hits were analysed by amino acid (aa) alignment and phylogenetic tree construction. Genes encoding KPC-2 homologues were expressed in Escherichia coli. The carbapenemase activities of the recombinant strains were characterized by the CarbaNP test and UV spectrophotometry and MICs of selected β-lactams were determined...
June 2016: Journal of Antimicrobial Chemotherapy
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