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https://www.readbyqxmd.com/read/28522882/monoclonal-suncus-antibodies-generation-of-fusion-partners-to-produce-suncus-suncus-hybridomas
#1
Yoshikazu Sado, Satoko Inoue, Yasuko Tomono, Makoto Matsuyama, Masaki Fukushima, Toshitaka Oohashi, Takamichi Jogahara, Sen-Ichi Oda
We used suncus (Suncus murinus; house musk shrew) to generate partner cells for cell fusion to produce suncus monoclonal antibodies. Suncus are insectivores that are genetically distant to rodents, and recognize antigens and epitopes that are not immunogenic in mice and rats, which are the animals most commonly used in basic life science research and from which monoclonal antibodies are usually produced. To date, monoclonal antibodies from suncus have not been generated due to the lack of a plasmacytoma fusion partner...
April 27, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28490374/a-serosurvey-of-selected-cystogenic-coccidia-in-spanish-equids-first-detection-of-anti-besnoitia-spp-specific-antibodies-in-europe
#2
Daniel Gutiérrez-Expósito, Ignacio García-Bocanegra, Daniel K Howe, Antonio Arenas-Montes, Michelle R Yeargan, SallyAnne L Ness, Luis M Ortega-Mora, G Álvarez-García
BACKGROUND: Equine besnoitiosis, caused by Besnoitia bennetti, and equine protozoal myeloencephalitis (EPM), caused by Sarcocystis neurona and Neospora hughesi are relevant equine diseases in the Americas that have been scarcely studied in Europe. Thus, a serosurvey of these cystogenic coccidia was carried out in Southern Spain. A cross-sectional study was performed and serum samples from horses (n = 553), donkeys (n = 85) and mules (n = 83) were included. An in-house enzyme-linked immunosorbent assay (ELISA) was employed to identify a Besnoitia spp...
May 10, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28479999/mitochondrial-function-and-bioenergetic-trade-offs-during-lactation-in-the-house-mouse-mus-musculus
#3
Annelise V Mowry, Zachary S Donoviel, Andreas N Kavazis, Wendy R Hood
Energy allocation theory predicts that a lactating female should alter the energetic demands of its organ systems in a manner that maximizes nutrient allocation to reproduction while reducing nutrient use for tasks that are not vital to immediate survival. We posit that organ-specific plasticity in the function of mitochondria plays a key role in mediating these energetic trade-offs. The goal of this project was to evaluate mitochondrial changes that occur in response to lactation in two of the most energetically demanding organs in the body of a rodent, the liver and skeletal muscle...
May 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28473258/representational-specializations-of-the-hippocampus-in-phylogenetic-perspective
#4
REVIEW
Elisabeth A Murray, Steven P Wise, Kim S Graham
In a major evolutionary transition that occurred more than 520 million years ago, the earliest vertebrates adapted to a life of mobile, predatory foraging guided by distance receptors concentrated on their heads. Vision and olfaction served as the principal sensory systems for guiding their search for nutrients and safe haven. Among their neural innovations, these animals had a telencephalon that included a homologue of the hippocampus. Experiments on goldfish, turtles, lizards, rodents, macaque monkeys and humans have provided insight into the initial adaptive advantages provided by the hippocampus homologue...
May 1, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28463690/impact-of-wear-and-diet-on-molar-row-geometry-and-topography-in-the-house-mouse
#5
Sabrina Renaud, Ronan Ledevin
OBJECTIVES: Dental evolution affects the geometry of the tooth, but the adaptive relevance of these changes is related to tooth sharpness, complexity, and relief (topography). On a set of laboratory mice, we assessed how wear related to age and food consistency affected molar geometry and topography. DESIGN: Three groups of laboratory inbred mice (C57BL/6J strain) were considered: Four week old mice close to weaning, six month old mice fed on regular rodent pellets, and six month old mice fed on rodent pellets that were powdered and served as jelly...
April 24, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28460593/the-role-of-rodents-in-avian-influenza-outbreaks-in-poultry-farms-a-review
#6
Francisca C Velkers, Simon J Blokhuis, Edwin J B Veldhuis Kroeze, Sara A Burt
Avian influenza outbreaks continue to strike poultry worldwide, affecting human and animal health and welfare and representing a substantial economic burden. Wild migratory birds, especially waterfowl and shore birds, are associated with global spread of avian influenza virus (AIV). Although direct contact with wild birds and virus-contaminated fomites is unlikely in modern non-free range poultry farms applying standard biosecurity measures, AIV outbreaks still occur. This suggests involvement of other intermediate factors for virus transmission between wild birds and poultry...
May 2, 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28452476/impact-of-processing-on-the-protein-quality-of-pinto-bean-phaseolus-vulgaris-and-buckwheat-fagopyrum-esculentum-moench-flours-and-blends-as-determined-by-in-vitro-and-in-vivo-methodologies
#7
Matthew G Nosworthy, Adam Franczyk, Anna Zimoch-Korzycka, Paulyn Appah, Alphonsus Utioh, Jason Neufeld, James D House
Blending of protein sources can increase protein quality by compensating for limiting amino acids present in individual sources, whereas processing grain flours by extrusion or baking can also alter protein quality. To determine the effect of baking and extrusion on the protein quality of blended flours from buckwheat and pinto beans, a rodent bioassay was performed and compared to an in vitro method of protein quality determination. Overall, extruded products had higher protein efficiency ratio values, increased digestibility, and greater protein digestibility corrected amino acid score (PDCAAS) values than baked products, with the extruded buckwheat/pinto blend having the greatest PDCAAS value of the experimental diets investigated...
May 9, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28447367/sex-hormones-function-as-sex-attractant-pheromones-in-house-mice-and-brown-rats
#8
Stephen Takacs, Regine Gries, Gerhard J Gries
Sex hormones of mammals control the expression of sexual characteristics and bodily functions. The male hormone testosterone and the female hormones progesterone and estradiol are known to occur in urine markings of mice. Herein, we show that all three hormones are also present in urine of brown rats, and that they are effective sexual communication signals (pheromones) that elicit attraction behavior of prospective mates in both brown rats and house mice. When added as lures to trap boxes in field experiments, synthetic testosterone, e...
April 26, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28433669/environmental-enrichment-reverses-memory-impairment-induced-by-toluene-in-mice
#9
Sergio Montes, Rocío Del Carmen Solís-Guillen, David García-Jácome, Nayeli Páez-Martínez
Toluene is the main component of a variety of inhalants that are used for intoxication purposes. Alterations in memory have been reported in inhalant users; however, it is unclear whether these impairments could be reversed, and the mechanisms involved in the putative recovery. Therefore, the main purpose of this study was to model the deleterious effects of toluene on memory in mice and to evaluate the effect of environmental enrichment on that response. In the second part of the study, the concentrations of glutamate and GABA, following chronic toluene exposure and after environmental enrichment treatment, were evaluated...
April 19, 2017: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/28433648/repeated-threat-without-direct-harm-alters-metabolic-capacity-in-select-regions-that-drive-defensive-behavior
#10
D J Kim, A S Lee, A A Yttredahl, R Gómez-Rodríguez, B J Anderson
To understand the behavioral consequences of intermittent anticipatory stress resulting from threats without accompanying physiological challenges, we developed a semi-naturalistic rodent housing and foraging environment that can include threats that are unpredictable in timing. Behavior is automatically recorded while rats forage for food or water. Over three weeks, the threats have been shown to elicit risk assessment behaviors, increase defensive burying and increase adrenal gland weight. To identify brain regions activated by this manipulation, we measured cytochrome c oxidase (COX), which is tightly coupled to neural activity...
April 19, 2017: Neuroscience
https://www.readbyqxmd.com/read/28432010/exposure-to-an-enriched-environment-facilitates-motor-recovery-and-prevents-short-term-memory-impairment-and-reduction-of-striatal-bdnf-in-a-progressive-pharmacological-model-of-parkinsonism-in-mice
#11
Clarissa L C Campêlo, José R Santos, Anatildes F Silva, Aline L Dierschnabel, André Pontes, Jeferson S Cavalcante, Alessandra M Ribeiro, Regina H Silva
Previous studies showed that the repeated administration with a low dose of reserpine (RES) induces a gradual appearance of motor signs and cognitive deficits compatible with parkinsonism in rodents. Environmental stimulation has neuroprotective effects in animal models of neurodegenerative damage, including acutely induced parkinsonism. We investigated the effects of exposure to an enriched environment (EE) on motor, cognitive and neuronal (levels of tyrosine hydroxylase, TH and brain derived neurotrophic factor, BDNF) deficits induced by a progressive model of Parkinson's disease (PD) in mice...
April 18, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28428867/adaptative-evolution-of-the-vkorc1-gene-in-mus-musculus-domesticus-is-influenced-by-the-selective-pressure-of-anticoagulant-rodenticides
#12
Joffrey Goulois, Véronique Lambert, Lionel Legros, Etienne Benoit, Virginie Lattard
Anticoagulant rodenticides are commonly used to control rodent pests worldwide. They specifically inhibit the vitamin K epoxide reductase (VKORC1), which is an enzyme encoded by the Vkorc1 gene, involved in the recycling of vitamin K. Therefore, they prevent blood clotting. Numerous mutations of Vkorc1 gene were reported in rodents, and some are involved in the resistant to rodenticides phenotype. Two hundred and sixty-six mice tails were received from 65 different locations in France. Coding sequences of Vkorc1 gene were sequenced in order to detect mutations...
April 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28425496/3-4-methylenedioxymethamphetamine-increases-affiliative-behaviors-in-squirrel-monkeys-in-a-serotonin-2a-receptor-dependent-manner
#13
Elizabeth G Pitts, Adelaide R Minerva, Erika B Oliver, Jordan N Kohn, Meghan T Logun, Agnieszka Sulima, Kenner C Rice, Leonard L Howell
3,4-methylenedioxymethamphetamine (MDMA) increases sociality in humans and animals. Release of serotonin (5-HT) is thought to play an important role in the increase in social behaviors, but the mechanisms underlying these effects are poorly understood. Despite the advantages of nonhuman primate models, no studies have examined the mechanisms of the social effects of MDMA in nonhuman primates. The behavior and vocalizations of four group-housed squirrel monkeys were examined following administration of MDMA, its enantiomers, and methamphetamine...
April 20, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28422070/one-year-follow-up-risk-assessment-in-skh-1-mice-and-wounds-treated-with-an-argon-plasma-jet
#14
Anke Schmidt, Thomas von Woedtke, Jan Stenzel, Tobias Lindner, Stefan Polei, Brigitte Vollmar, Sander Bekeschus
Multiple evidence in animal models and in humans suggest a beneficial role of cold physical plasma in wound treatment. Yet, risk assessment studies are important to further foster therapeutic advancement and acceptance of cold plasma in clinics. Accordingly, we investigated the longterm side effects of repetitive plasma treatment over 14 consecutive days in a rodent full-thickness ear wound model. Subsequently, animals were housed for 350 days and sacrificed thereafter. In blood, systemic changes of the proinflammatory cytokines interleukin 1β and tumor necrosis factor α were absent...
April 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28418764/disease-surveillance-of-california-ground-squirrels-spermophilus-beecheyi-in-a-drive-through-zoo-in-oregon-usa
#15
Julia Ter Beest, Andrew Cushing, Modesto McClean, Wendy Hsu, Robert Bildfell
Rodents and other small wild mammals are often considered to be pests and vectors for disease in zoos that house small populations of valuable threatened and endangered animals. In 2005, three nonhuman primates at a drive-through zoo in Oregon, USA, acquired tularemia from an unknown source. Due to an abundance of California ground squirrels ( Spermophilus beecheyi ) on zoo grounds, we instituted serosurveillance of this species from July through September 2008 to determine the prevalence of antibodies against pathogens considered to be potentially transmissible to collection animals...
April 18, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28418312/exposure-characteristics-of-hantavirus-pulmonary-syndrome-patients-united-states-1993-2015
#16
Annabelle de St Maurice, Elizabeth Ervin, Mare Schumacher, Hayley Yaglom, Elizabeth VinHatton, Sandra Melman, Ken Komatsu, Jennifer House, Dallin Peterson, Danielle Buttke, Alison Ryan, Del Yazzie, Craig Manning, Paul Ettestad, Pierre Rollin, Barbara Knust
Those at highest risk are persons in occupations with potential for rodent exposure and American Indian women 40--64 years of age.
May 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28407853/effects-of-environmental-enrichment-on-reproductive-performance-and-quantity-and-morphology-of-cumulus-oocyte-complexes-obtained-from-rattus-norvegicus
#17
Joana Fisch, Iáskara Vieira de Oliveira, Juliana Fank, Lia Mara Gomes Paim, Marília Remuzzi Zandoná, Eliana Franco Lopes, Fernanda Bastos de Mello, Alexandre Tavares Duarte de Oliveira
Several researchers have observed that environmental enrichment (EE) can be effective in reducing stressful conditions and abnormal behavior and may provide better reproductive performance in rodents. In this context, this study aimed to evaluate the reproductive performance of Wistar rats reared in three different housing conditions. Animals were separated into breeding pairs, one pair per cage and pairs randomly assigned to three experimental groups (ten couples per group): control group were provided cages without any environmental enrichment; PVC group with PVC pipe; and cardboard roll group with a commercially available cardboard tube...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28399925/human-serum-rnase-l-level-is-inversely-associated-with-metabolic-syndrome-and-age
#18
Yi-Ting Wang, Ping-Huei Tseng, Chi-Ling Chen, Der-Sheng Han, Yu-Chiao Chi, Fen-Yu Tseng, Wei-Shiung Yang
BACKGROUND: Ribonuclease-L (RNase-L) was known to be a ubiquitous enzyme involved in several cellular functions, especially innate immunity. It was recently shown to participate in adipogenesis in rodents. Here, we developed a method to measure serum levels of RNase-L and analyzed the relationship between RNase-L and metabolic syndrome (MetS). METHODS: A total of 396 subjects were recruited from a health check-up program. An in-house RNase-L immunoassay was developed...
April 11, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/28397512/evaluation-of-perlite-wood-shavings-and-corncobs-for-bedding-material-in-rats
#19
Fatih Yildirim, Betül A Yildirim, Ahmet Yildiz, Kübra A Kapakin Terim, Seyda Cengiz, Selçuk Özdemir
Bedding material, which is a significant part of rodent housing, affects the health and well-being of laboratory animals. The aim of this study was to evaluate perlite as a bedding material for rodents and to compare it with wood shavings, expanded perlite and corncobs. The animals used in this experiment were 48 male and 48 female Sprague-Dawley rats. The bedding materials collected from experimental groups were analysed microbiologically. Blood samples from rats were subjected to biochemical analysis for catalase, glutathione, glutathione peroxidase, malondialdehyde, superoxide and dismutase, and foot pad skins of rats were subjected to histopathological examination...
March 30, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28378204/a-schizophrenia-relevant-5-choice-serial-reaction-time-task-for-mice-assessing-broad-monitoring-distractibility-and-impulsivity
#20
Huiping Huang, Simone Guadagna, Maddalena Mereu, Mariasole Ciampoli, Giacomo Pruzzo, Theresa Ballard, Francesco Papaleo
The 5-Choice Serial Reaction Time Task (5-CSRTT) is an automated test for rodents allowing the assessment of multiple cognitive measures. Originally designed to assess cognitive deficits relevant to attention deficit hyperactivity disorder, it has been widely used in the investigation of neural systems of attention. In the current study, we have set up a modified version, which reduced the training phase to only 8-9 days with minimal food deprivation and without single-housing. Furthermore, based on evidence that patients with schizophrenia are more impaired in broad monitoring abilities than in sustained attention, we successfully developed a protocol replicating the Spatial Attentional Resource Allocation Task (SARAT), used in humans to assess broad monitoring...
April 5, 2017: Psychopharmacology
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