Ana Paula Serafini Poeta Silva, Robert Mugabi, Marisa L Rotolo, Seth Krantz, Dapeng Hu, Rebecca Robbins, Deanne Hemker, Andres Diaz, A W Tucker, Rodger Main, Jean Paul Cano, Perry Harms, Chong Wang, Maria Jose Clavijo
Tracheal pooling for Mycoplasma hyopneumoniae (M. hyopneumoniae) DNA detection allows for decreased diagnostic cost, one of the main constraints in surveillance programs. The objectives of this study were to estimate the sensitivity of pooled-sample testing for the detection of M. hyopneumoniae in tracheal samples and to develop probability of M. hyopneumoniae detection estimates for tracheal samples pooled by 3, 5, and 10. A total of 48 M. hyopneumoniae PCR-positive field samples were pooled 3-, 5-, and 10-times using field M...
May 3, 2024: Scientific Reports