keyword
MENU ▼
Read by QxMD icon Read
search

Environmental enrichment

keyword
https://www.readbyqxmd.com/read/28641137/real-time-pcr-methods-for-the-detection-of-blown-pack-spoilage-causing-clostridium-species-c-estertheticum-c-gasigenes-and-c-ruminantium
#1
Rachael Reid, Catherine M Burgess, Evonne McCabe, Séamus Fanning, Paul Whyte, Joe Kerry, Declan Bolton
A set of real-time PCR methods for the detection of C. estertheticum, C. gasigenes and C. ruminantium, the causative agents of blown pack spoilage (BPS) in vacuum packaged beef, was developed. Robust validation of the sensitivity and specificity was carried out in the three matrices (beef meat drip, wet environmental swabs and dry environmental swabs) as encountered in our testing laboratory and against Clostridium strains (n=76) and non-Clostridium strains (n=36). It was possible to detect 4-5 spores per ml for C...
June 3, 2017: Meat Science
https://www.readbyqxmd.com/read/28640897/impact-of-selected-non-steroidal-anti-inflammatory-pharmaceuticals-on-microbial-community-assembly-and-activity-in-sequencing-batch-reactors
#2
Cong Jiang, Jinju Geng, Haidong Hu, Haijun Ma, Xingsheng Gao, Hongqiang Ren
This study covers three widely detected non-steroidal anti-inflammatory pharmaceuticals (NSAIDs), diclofenac (DCF), ibuprofen (IBP) and naproxen (NPX), as NSAIDs pollutants. The objective is to evaluate the impact of NSAIDs at their environmental concentrations on microbial community assembly and activity. The exposure experiments were conducted under three conditions (5 μg L-1 DCF, 5 μg L-1 DCF+5 μg L-1 IBP and 5 μg L-1 DCF+5 μg L-1 IBP+ 5 μg L-1 NPX) in sequencing batch reactors (SBRs) for 130 days...
2017: PloS One
https://www.readbyqxmd.com/read/28637318/experience-dependent-regulation-of-cajal-retzius-cell-networks-in-the-developing-and-adult-mouse-hippocampus
#3
Max Anstötz, Sun Kyong Lee, Tamra I Neblett, Gabriele M Rune, Gianmaria Maccaferri
In contrast to their near-disappearance in the adult neocortex, Cajal-Retzius cells have been suggested to persist longer in the hippocampus. A distinctive feature of the mature hippocampus, not maintained by other cortical areas, is its ability to sustain adult neurogenesis. Here, we have investigated whether environmental manipulations affecting hippocampal postnatal neurogenesis have a parallel impact on Cajal-Retzius cells. We used multiple mouse reporter lines to unequivocally identify Cajal-Retzius cells and quantify their densities during postnatal development...
June 16, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28634817/identification-of-the-acclimation-genes-in-transcriptomic-responses-to-heat-stress-of-white-pekin-duck
#4
Jun-Mo Kim, Kyu-Sang Lim, Mijeong Byun, Kyung-Tai Lee, Young-Rok Yang, Mina Park, Dajeong Lim, Han-Ha Chai, Han-Tae Bang, Jong Hwangbo, Yang-Ho Choi, Yong-Min Cho, Jong-Eun Park
White Pekin duck is an important meat resource in the livestock industries. However, the temperature increase due to global warming has become a serious environmental factor in duck production, because of hyperthermia. Therefore, identifying the gene regulations and understanding the molecular mechanism for adaptation to the warmer environment will provide insightful information on the acclimation system of ducks. This study examined transcriptomic responses to heat stress treatments (3 and 6 h at 35 °C) and control (C, 25 °C) using RNA-sequencing analysis of genes from the breast muscle tissue...
June 20, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28634240/leucine-biosynthesis-is-involved-in-regulating-high-lipid-accumulation-in-yarrowia-lipolytica
#5
Eduard J Kerkhoven, Young-Mo Kim, Siwei Wei, Carrie D Nicora, Thomas L Fillmore, Samuel O Purvine, Bobbie-Jo Webb-Robertson, Richard D Smith, Scott E Baker, Thomas O Metz, Jens Nielsen
The yeast Yarrowia lipolytica is a potent accumulator of lipids, and lipogenesis in this organism can be influenced by a variety of factors, such as genetics and environmental conditions. Using a multifactorial study, we elucidated the effects of both genetic and environmental factors on regulation of lipogenesis in Y. lipolytica and identified how two opposite regulatory states both result in lipid accumulation. This study involved comparison of a strain overexpressing diacylglycerol acyltransferase (DGA1) with a control strain grown under either nitrogen or carbon limitation conditions...
June 20, 2017: MBio
https://www.readbyqxmd.com/read/28633843/ultrasound-assisted-dispersive-magnetic-solid-phase-extraction-for-preconcentration-and-determination-of-trace-amount-of-hg-ii-ions-from-food-samples-and-aqueous-solution-by-magnetic-graphene-oxide-fe3o4-go-2-ptsc-central-composite-design-optimization
#6
Akram Keramat, Rouholah Zare-Dorabei
In this work, the synthesis of the magnetic graphene oxide modified by 2-pyridinecarboxaldehyde thiosemicarbazone groups (Fe3O4@GO/2-PTSC) was utilized for preconcentration and determination of mercuric ions in a trace amount by inductively coupled plasma-optical emission spectrometry (ICP-OES). Characterization of the adsorbent was performed using various techniques, such as FT-IR, VSM, SEM and XRD analysis. Central composite design (CCD) under response surface methodology (RSM) was used for obtaining the most important parameters and probable interactions in variables...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28633360/expression-properties-exhibit-correlated-patterns-with-the-fate-of-duplicated-genes-their-divergence-and-transcriptional-plasticity-in-saccharomycotina
#7
Florian Mattenberger, Beatriz Sabater-Muñoz, Christina Toft, Gaurav Sablok, Mario A Fares
Gene duplication is an important source of novelties and genome complexity. What genes are preserved as duplicated through long evolutionary times can shape the evolution of innovations. Identifying factors that influence gene duplicability is therefore an important aim in evolutionary biology. Here, we show that in the yeast Saccharomyces cerevisiae the levels of gene expression correlate with gene duplicability, its divergence, and transcriptional plasticity. Genes that were highly expressed before duplication are more likely to be preserved as duplicates for longer evolutionary times and wider phylogenetic ranges than genes that were lowly expressed...
June 15, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28631837/transcriptional-and-histological-alterations-in-gonad-of-adult-zebrafish-after-exposure-to-the-synthetic-progestin-norgestrel
#8
Wen-Jun Shi, Jian-Liang Zhao, Yu-Xia Jiang, Guo-Yong Huang, You-Sheng Liu, Jin-Na Zhang, Guang-Guo Ying
The aim of this study was to investigate the effects of Norgestrel (NGT) on the gonadal development in adult zebrafish. Adult zebrafish were exposed to NGT for 14 days at 871 ng L(-1) for microarray analysis, and a follow-up experiment was conducted to further study targeted pathway in adult zebrafish after exposure to NGT at 6.7 ng L(-1) , 83 ng L(-1) and 912 ng L(-1) by quantitative PCR (qPCR) and histology analysis. The microarray analysis revealed that 11545 transcripts were identified. Gene ontology analysis showed organ development, system development, multicellular organismal development, single-organism developmental process and developmental process were significantly enriched...
June 20, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28631591/comparative-evaluation-of-veriflow-%C3%A2-listeria-species-to-usda-culture-based-method-for-the-detection-of-listeria-spp-in-food-and-environmental-samples
#9
Adam C Joelsson, Shawn P Terkhorn, Ashley S Brown, Amrita Puri, Benjamin J Pascal, Zara E Gaudioso, Nicholas A Siciliano
Veriflow(®)Listeria species (Veriflow LS) isa molecular-based assay for the presumptive detection of Listeria spp. from environmental surfaces (stainless steel, sealed concrete, plastic, and ceramic tile) and ready-to-eat (RTE) food matrixes (hot dogs and deli meat). The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post-PCR amplification and requires only a 24 h enrichment for maximum sensitivity. The Veriflow LS system eliminates the need for sample purification, gel electrophoresis, or fluorophore-based detection of target amplification and does not require complex data analysis...
June 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28631590/comparative-evaluation-of-veriflow-%C3%A2-salmonella-species-to-usda-and-fda-culture-based-methods-for-the-detection-of-salmonella-spp-in-food-and-environmental-samples
#10
Amrita Puri, Adam C Joelsson, Shawn P Terkhorn, Ashley S Brown, Zara E Gaudioso, Nicholas A Siciliano
Veriflow(®)Salmonella species (Veriflow SS)is a molecular-based assay for the presumptive detection of Salmonella spp. from environmental surfaces (stainless steel, sealed concrete, plastic, and ceramic tile), dairy (2% milk), raw meat (20% fat ground beef), chicken carcasses, and ready-to-eat (RTE) food (hot dogs). The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post-PCR amplification and requires only an 18 h enrichment for maximum sensitivity...
June 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28630595/qsar-models-of-human-data-can-enrich-or-replace-llna-testing-for-human-skin-sensitization
#11
Vinicius M Alves, Stephen J Capuzzi, Eugene Muratov, Rodolpho C Braga, Thomas Thornton, Denis Fourches, Judy Strickland, Nicole Kleinstreuer, Carolina H Andrade, Alexander Tropsha
Skin sensitization is a major environmental and occupational health hazard. Although many chemicals have been evaluated in humans, there have been no efforts to model these data to date. We have compiled, curated, analyzed, and compared the available human and LLNA data. Using these data, we have developed reliable computational models and applied them for virtual screening of chemical libraries to identify putative skin sensitizers. The overall concordance between murine LLNA and human skin sensitization responses for a set of 135 unique chemicals was low (R = 28-43%), although several chemical classes had high concordance...
December 21, 2016: Green Chemistry: An International Journal and Green Chemistry Resource: GC
https://www.readbyqxmd.com/read/28629963/environmental-enrichment-reduces-innate-anxiety-with-no-effect-on-depression-like-behaviour-in-mice-lacking-the-serotonin-transporter
#12
Jake Rogers, Shanshan Li, Laurence Lanfumey, Anthony J Hannan, Thibault Renoir
Along with being the main target of many antidepressant medications, the serotonin transporter (5-HTT) is known to be involved in the pathophysiology of depression and anxiety disorders. In line with this, mice with varying 5-HTT genotypes are invaluable tools to study depression- and anxiety-like behaviours as well as the mechanisms mediating potential therapeutics. There is clear evidence that both genetic and environmental factors play a role in the aetiology of psychiatric disorders. In that regard, housing paradigms which seek to enhance cognitive stimulation and physical activity have been shown to exert beneficial effects in animal models of neuropsychiatric disorders...
June 16, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28627812/genetics-of-alcohol-consumption-in-drosophila-melanogaster
#13
Sophia Fochler, Tatiana V Morozova, Morgan R Davis, Alexander W Gearhart, Wen Huang, Trudy F C Mackay, Robert R H Anholt
Individual variation in alcohol consumption in human populations is determined by genetic, environmental, social and cultural factors. In contrast to humans, genetic contributions to complex behavioral phenotypes can be readily dissected in Drosophila, where both the genetic background and environment can be controlled and behaviors quantified through simple high-throughput assays. Here, we measured voluntary consumption of ethanol in ~3,000 individuals of each sex from an advanced intercross population derived from 37 lines of the Drosophila melanogaster Genetic Reference Panel...
June 19, 2017: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/28627010/a-turtle-cognition-research-demonstration-enhances-visitor-engagement-and-keeper-animal-relationships
#14
Andrew C Alba, Katherine A Leighty, Victoria L Pittman Courte, Alison P Grand, Tamara L Bettinger
Environmental enrichment techniques present animals with cognitive challenges while providing them opportunities to make choices and exert control over their environment. In this way, cognitive research and training is enriching to animals and can be used as a form of enrichment in zoos and aquariums. Cognitive research demonstrations also provide an opportunity to enhance visitor experience, as well as foster interactions between animals and keepers. We investigated how cognitive research sessions involving eastern box turtles (Terrapene carolina carolina) at Disney's Animal Kingdom(®) impacted both the rate of visitors coming to the exhibit and the amount of time they spent engaged...
June 18, 2017: Zoo Biology
https://www.readbyqxmd.com/read/28626452/proteomic-differences-between-developmental-stages-of-toxoplasma-gondii-revealed-by-itraq-based-quantitative-proteomics
#15
Ze-Xiang Wang, Chun-Xue Zhou, Hany M Elsheikha, Shuai He, Dong-Hui Zhou, Xing-Quan Zhu
Toxoplasma gondii has a complex two-host life-cycle between intermediate host and definitive host. Understanding proteomic variations across the life-cycle stages of T. gondii may improve the understanding of molecular adaption mechanism of T. gondii across life-cycle stages, and should have implications for the development of new treatment and prevention interventions against T. gondii infection. Here, we utilized LC-MS/MS coupled with iTRAQ labeling technology to identify differentially expressed proteins (DEPs) specific to tachyzoite (T), bradyzoites-containing cyst (C) and sporulated oocyst (O) stages of the cyst-forming T...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28626137/novel-palm-fatty-acid-functionalized-magnetite-nanoparticles-for-magnetic-solid-phase-extraction-of-trace-polycyclic-aromatic-hydrocarbons-from-environmental-samples
#16
Siti Khalijah Mahmad Rozi, Hamid Rashidi Nodeh, Muhammad Afzal Kamboh, Ninie Suhana Abdul Manan, Sharifah Mohamad
A novel adsorbent, palm fatty acid coated magnetic Fe3O4 nanoparticles (MNP-FA) was successfully synthesized with immobilization of the palm fatty acid onto the surface of MNPs. The successful synthesis of MNP-FA was further confirmed by X-Ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and Energy dispersive X-Ray spectroscopy (EDX) analyses and water contact angle (WCA) measurement. This newly synthesized MNP-FA was applied as magnetic solid phase extraction (MSPE) adsorbent for the enrichment of polycyclic aromatic hydrocarbons (PAHs), namely fluoranthene (FLT), pyrene (Pyr), chrysene (Cry) and benzo(a)pyrene (BaP) from environmental samples prior to High Performance Liquid Chromatography- Diode Array Detector (HPLC-DAD) analysis...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28624629/zinc-oxide-nanoparticle-exposure-triggers-different-gene-expression-patterns-in-maize-shoots-and-roots
#17
Hongwei Xun, Xintong Ma, Jing Chen, Zhongzhou Yang, Bao Liu, Xiang Gao, Guo Li, Jiamiao Yu, Li Wang, Jinsong Pang
The potential impacts of environmentally accumulated zinc oxide nanoparticles (nZnOs) on plant growth have not been well studied. A transcriptome profile analysis of maize exposed to nZnOs showed that the genes in the shoots and roots responded differently. Although the number of differentially expressed genes (DEGs) in the roots was greater than that in the shoots, the number of up- or down-regulated genes in both the shoots and roots was similar. The enrichment of gene ontology (GO) terms was also significantly different in the shoots and roots...
June 15, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28624468/fish-mass-immunization-against-virus-with-recombinant-spiny-bacterins
#18
Julio Coll
Bacterins obtained from recombinant bacteria displaying heterologous antigens in its surface coded by prokaryotic rather than eukaryotic expression plasmids (here called "spiny" bacterins or spinycterins), have been used to increase fish immunogenicity of recombinant viral protein fragments. To explore their immunogenicity, five bacterial-specific membrane anchor-motifs characterized in the literature (Nmistic, Mistic, NTD, YAIN and YBEL) were genetically fused to the immunorelevant cystein-free 117 amino acid fragment II from the ORF149 of cyprinid herpes virus 3 (frgIICyHV3)...
June 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28624316/reversal-learning-in-c58-mice-modeling-higher-order-repetitive-behavior
#19
Cristina M Whitehouse, Lisa S Curry-Pochy, Robin Shafer, Joseph Rudy, Mark H Lewis
Restricted, repetitive behaviors are diagnostic for autism and prevalent in other neurodevelopmental disorders. These behaviors cluster as repetitive sensory-motor behaviors and behaviors reflecting resistance to change. The C58 mouse strain is a promising model for these behaviors as it emits high rates of aberrant repetitive sensory-motor behaviors. The purpose of the present study was to extend characterization of the C58 model to resistance to change. This was done by comparing C58 to C57BL/6 mice on a reversal learning task under either a 100% or 80%/20% probabilistic reinforcement schedule...
June 14, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28623920/ketamine-induced-apoptosis-in-the-mouse-cerebral-cortex-follows-similar-characteristic-of-physiological-apoptosis-and-can-be-regulated-by-neuronal-activity
#20
Qi Wang, Feng-Yan Shen, Rong Zou, Jing-Jing Zheng, Xiang Yu, Ying-Wei Wang
The effects of general anesthetics on inducing neuronal apoptosis during early brain development are well-documented. However, since physiological apoptosis also occurs during this developmental window, it is important to determine whether anesthesia-induced apoptosis targets the same cell population as physiological apoptosis or different cell types altogether. To provide an adequate plane of surgery, ketamine was co-administered with dexmedetomidine. The apoptotic neurons in the mouse primary somatosensory cortex (S1) were quantitated by immunohistochemistry...
June 17, 2017: Molecular Brain
keyword
keyword
22368
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"