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https://www.readbyqxmd.com/read/28108753/release-of-free-amino-acids-upon-oxidation-of-peptides-and-proteins-by-hydroxyl-radicals
#1
Fobang Liu, Senchao Lai, Haijie Tong, Pascale S J Lakey, Manabu Shiraiwa, Michael G Weller, Ulrich Pöschl, Christopher J Kampf
Hydroxyl radical-induced oxidation of proteins and peptides can lead to the cleavage of the peptide, leading to a release of fragments. Here, we used high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) and pre-column online ortho-phthalaldehyde (OPA) derivatization-based amino acid analysis by HPLC with diode array detection and fluorescence detection to identify and quantify free amino acids released upon oxidation of proteins and peptides by hydroxyl radicals. Bovine serum albumin (BSA), ovalbumin (OVA) as model proteins, and synthetic tripeptides (comprised of varying compositions of the amino acids Gly, Ala, Ser, and Met) were used for reactions with hydroxyl radicals, which were generated by the Fenton reaction of iron ions and hydrogen peroxide...
January 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28107925/green-synthesized-co-nanoparticles-doped-amino-graphene-modified-electrode-and-its-application-towards-determination-of-baicalin
#2
Kai Sheng, Lu Wang, Huichao Li, Lina Zou, Baoxian Ye
The present paper reported an one-pot synthesis of Co nanoparticles doped amino-graphene nanocomposites (Co-amino-Gr), involving in situ generation of Co nanoparticles (NPs). The simultaneous reduction of graphene oxide (GO) and Co(2+) to produce Co-amino-Gr had been achieved under mild reaction conditions using an environmentally benign reducing agent, glycine. By combining the merits of amino-Gr and the Co NPs, a highly sensitive electrochemical sensor was erected to detect baicalin based on the Co-amino-Gr nanocomposites...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107733/glycine-increases-cold-tolerance-in-rice-via-the-regulation-of-n-uptake-physiological-characteristics-and-photosynthesis
#3
Cao Xiaochuang, Zhong Chu, Zhu Lianfeng, Zhang Junhua, Sajid Hussain, Wu Lianghuan, Jin Qianyu
To investigate the response of rice growth and photosynthesis to different nitrogen (N) sources under cold stress, hydroponic cultivation of rice was done in greenhouse, with glycine, ammonium, and nitrate as the sole N sources. The results demonstrate that exposure to low temperature reduced the rice biomass and leaf chlorophyll content, but their values in the glycine-treated plants were significantly higher than in the ammonium- and nitrate-treated plants. This might be attributed to the higher N uptake rate and root area and activity in the glycine-treated plants...
January 12, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28107674/mitochondrial-serine-hydroxymethyltransferase-2-is-a-potential-diagnostic-and-prognostic-biomarker-for-human-glioma
#4
Bo Wang, Wei Wang, ZhiZhong Zhu, XueBin Zhang, Fan Tang, Dong Wang, Xi Liu, XiaoLing Yan, Hao Zhuang
OBJECTIVE: Scholars have gradually come to appreciate the relevance of serine and glycine metabolism. Recently, researchers have discovered that mitochondrial serine hydroxymethyltransferase 2 (SHMT2) is overexpressed in various types of cancer. However, the function of SHMT2 in glioma is not clear. In this study, we sought to examine the expression of SHMT2 in glioma, the association between SHMT2 expression and clinicopathological characteristics, and the association of SHMT2 expression with prognosis in glioma patients...
January 16, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28106735/mass-spectrometry-based-metabolomic-and-lipidomic-analyses-of-the-effects-of-dietary-platycodon-grandiflorum-on-liver-and-serum-of-obese-mice-under-a-high-fat-diet
#5
Hye Min Park, Kab-Tae Park, Edmond Changkyun Park, Seung Ii Kim, Myung Sook Choi, Kwang-Hyeon Liu, Choong Hwan Lee
We aimed to identify metabolites involved in the anti-obesity effects of Platycodon grandiflorum (PG) in high-fat diet (HFD)-fed mice using mass spectrometry (MS)-based metabolomic techniques. C57BL/6J mice were divided into four groups: normal diet (ND)-fed mice, HFD-fed mice, HFD with 1% PG extract-fed mice (HPGL), and HFD with 5% PG extract-fed mice (HPGH). After 8 weeks, the HFD group gained more weight than the ND group, while dietary 5% PG extract attenuated this change. The partial least squares discriminant analysis (PLS-DA) score plots showed a clear distinction between experimental groups in serum and liver markers...
January 17, 2017: Nutrients
https://www.readbyqxmd.com/read/28106251/affinity-capillary-electrophoresis-and-quantum-mechanical-calculations-applied-to-investigation-of-gly-6-antamanide-binding-with-sodium-and-potassium-ions
#6
Sachin Pangavhane, Stanislav Böhm, Emanuel Makrlík, Paolo Ruzza, Václav Kašička
Affinity capillary electrophoresis in a free solution (ACE) and quantum mechanical density functional theory (DFT) have been applied to the investigation of interactions of glycine-6-antamanide ([Gly(6) ]AA), a synthetic derivative of cyclic decapeptide antamanide isolated from the highly poisonous mushroom Amanita phalloides, with sodium or potassium ions in methanol. First, from the dependence of effective electrophoretic mobility of [Gly(6) ]AA on Na(+) or K(+) ions concentration in the background electrolyte (BGE) (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pHMeOH 7...
January 20, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28105955/small-rna-profiles-in-soybean-primary-root-tips-under-water-deficit
#7
Yun Zheng, Vandana Hivrale, Xiaotuo Zhang, Babu Valliyodan, Christine Lelandais-Brière, Andrew D Farmer, Gregory D May, Martin Crespi, Henry T Nguyen, Ramanjulu Sunkar
BACKGROUND: Soybean (Glycine max) production is significantly hampered by frequent droughts in many regions of the world including the United States. Identifying microRNA (miRNA)-controlled posttranscriptional gene regulation under drought will enhance our understanding of molecular basis of drought tolerance in this important cash crop. Indeed, miRNA profiles in soybean exposed to drought were studied but not from the primary root tips, which is not only a main zone of water uptake but also critical for water stress sensing and signaling...
December 5, 2016: BMC Systems Biology
https://www.readbyqxmd.com/read/28105280/glun2a-selective-pyridopyrimidinone-series-of-nmdar-positive-allosteric-modulators-with-an-improved-in-vivo-profile
#8
Elisia Villemure, Matthew Volgraf, Yu Jiang, Guosheng Wu, Cuong Q Ly, Po-Wai Yuen, Aijun Lu, Xifeng Luo, Mingcui Liu, Shun Zhang, Patrick J Lupardus, Heidi J A Wallweber, Bianca M Liederer, Gauri Deshmukh, Emile Plise, Suzanne Tay, Tzu-Ming Wang, Jesse E Hanson, David H Hackos, Kimberly Scearce-Levie, Jacob B Schwarz, Benjamin D Sellers
The N-methyl-d-aspartate receptor (NMDAR) is an ionotropic glutamate receptor, gated by the endogenous coagonists glutamate and glycine, permeable to Ca(2+) and Na(+). NMDAR dysfunction is associated with numerous neurological and psychiatric disorders, including schizophrenia, depression, and Alzheimer's disease. Recently, we have disclosed GNE-0723 (1), a GluN2A subunit-selective and brain-penetrant positive allosteric modulator (PAM) of NMDARs. This work highlights the discovery of a related pyridopyrimidinone core with distinct structure-activity relationships, despite the structural similarity to GNE-0723...
January 12, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28105238/immune-associated-proteins-with-potential-in-vivo-anti-tumor-activities-are-upregulated-in-lung-cancer-cells-treated-with-umbelliprenin-a-proteomic-approach
#9
Narges Khaghanzadeh, Kazuyuki Nakamura, Yasuhiro Kuramitsu, Abbas Ghaderi, Zahra Mojtahedi
Umbelliprenin (Umb), a natural coumarin, has demonstrated anti-tumor activities, both in vitro and particularly in vivo, in several types of cancer, including lung cancer. The present study aimed to identify molecular targets of Umb using a high-throughput approach. Lung cancer cell lines, QU-DB (large-cell lung carcinoma) and A549 (adenocarcinoma), were treated with Umb. Differentially-expressed proteins were identified using two-dimensional electrophoresis coupled to mass spectrometry. In the QU-DB cells, differential expression of proteins, including downregulation of the tumorigenic protein heat shock protein 90 kDa and upregulation of the potential anti-tumor proteins Nipsnap1 and glycine-tRNA ligase (GRS), suggested that Umb is a strong anti-tumor compound...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28104923/unique-heating-curves-generated-by-radiofrequency-electric-field-interactions-with-semi-aqueous-solutions
#10
Nadia C Lara, Asad A Haider, Lon J Wilson, Steven A Curley, Stuart J Corr
Aqueous and nanoparticle-based solutions have been reported to heat when exposed to an alternating radiofrequency (RF) electric-field. Although the theoretical models have been developed to accurately model such a behavior given the solution composition as well as the geometrical constraints of the sample holder, these models have not been investigated across a wide-range of solutions where the dielectric properties differ, especially with regard to the real permittivity. In this work, we investigate the RF heating properties of non-aqueous solutions composed of ethanol, propylene glycol, and glycine betaine with and without varying amounts of NaCl and LiCl...
January 2, 2017: Applied Physics Letters
https://www.readbyqxmd.com/read/28102596/delineation-of-ehlers-danlos-syndrome-phenotype-due-to-the-c-934c-t-p-arg312cys-mutation-in-col1a1-report-on-a-three-generation-family-without-cardiovascular-events-and-literature-review
#11
Marina Colombi, Chiara Dordoni, Marina Venturini, Arianna Zanca, Piergiacomo Calzavara-Pinton, Marco Ritelli
Classical Ehlers-Danlos syndrome (cEDS) is a rare connective tissue disorder primarily characterized by hyperextensible skin, defective wound healing, abnormal scars, easy bruising, and generalized joint hypermobility; arterial dissections are rarely observed. Mutations in COL5A1 and COL5A2 encoding type V collagen account for more than 90% of the patients so far characterized. In addition, cEDS phenotype was reported in a small number of patients carrying the c.934C>T mutation in COL1A1 that results in an uncommon substitution of a non-glycine residue in one Gly-Xaa-Yaa repeat of the pro-α1(I)-chain p...
February 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28102497/regulated-partitioning-of-fixed-carbon-14-c-sodium-na-potassium-k-and-glycine-betaine-determined-salinity-stress-tolerance-of-gamma-irradiated-pigeonpea-cajanus-cajan-l-millsp
#12
Pankaj Kumar, Vasundhara Sharma, Chobhe Kapil Atmaram, Bhupinder Singh
Soil salinity is a major constraint that limits legume productivity. Pigeonpea is a salt sensitive crop. Seed gamma irradiation at a very low dose (2.5 Gy) is known to enhance seedling establishment, plant growth and yield of cereals and other crops. The present study conducted using two genetically diverse varieties of pigeonpea viz., Pusa-991 and Pusa-992 aimed at establishing the role of pre-sowing seed gamma irradiation at 0, 0.0025, 0.005, 0.01, 0.02, 0.05 and 0.1 kGy on plant growth, seed yield and seed quality under salt stress at 0, 80 and 100 mM NaCl (soil solution EC equivalent 1...
January 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28102080/n-oleoylglycine-induced-hyperphagia-was-associated-with-the-activation-of-agrp-neuron-by-cb1r
#13
Junguo Wu, Canjun Zhu, Liusong Yang, Zhonggang Wang, Lina Wang, Songbo Wang, Ping Gao, Yongliang Zhang, Qingyan Jiang, Gang Shu, Xiaotong Zhu
N-acyl amino acids (NAAAs) are conjugate products of fatty acids and amino acids, which are available in animal-derived food. We compared the effects of N-arachidonoyl glycine (NAGly), N-arachidonoyl serine (NASer), and N-Oleoylglycine (OLGly) on in vivo food intake and in vitro [Ca2+]i of AgRP neurons to identify the role of these compounds in energy homeostasis. The hypothalamic neuropeptide expression and anxiety behavior in response to OLGly were also tested. To further identify the underlying mechanism of OLGly on food intake, we first detected the expression level of potential OLGly receptors...
January 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28101463/development-of-a-novel-human-single-chain-antibody-against-egfrviii-antigen-by-phage-display-technology
#14
Leila Rahbarnia, Safar Farajnia, Hossein Babaei, Jafar Majidi, Bahman Akbari, Shiva Ahdi Khosroshahi
Purpose: EGFRvIII as the most common mutant variant of the epidermal growth factor receptor is resulting from deletion of exons 2-7 in the coding sequence and junction of exons 1 and 8 through a novel glycine residue. EGFRvIII is highly expressed in glioblastoma, carcinoma of the breast, ovary, and lung but not in normal cells. The aim of the present study was identification of a novel single chain antibody against EGFRvIII as a promising target for cancer therapy. Methods: In this study, a synthetic peptide corresponding to EGFRvIII protein was used for screening a naive human scFv phage library...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28100213/transcriptomic-response-of-wolf-spider-pardosa-pseudoannulata-to-transgenic-rice-expressing-bacillus-thuringiensis-cry1ab-protein
#15
Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song, Xuguo Zhou
BACKGROUND: Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthetic insecticides. Non-target organisms can be exposed to Bt toxins through direct feeding or trophic interactions in the field. The wolf spider Pardosa pseudoannulata, one of the dominant predators in South China, plays a crucial role in the rice agroecosystem. In this study, we investigated transcriptome responses of the 5th instar spiders fed on preys maintained on Bt- and non-Bt rice...
January 18, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28100173/whole-genome-wide-transcript-profiling-to-identify-differentially-expressed-genes-associated-with-seed-field-emergence-in-two-soybean-low-phytate-mutants
#16
Fengjie Yuan, Xiaomin Yu, Dekun Dong, Qinghua Yang, Xujun Fu, Shenlong Zhu, Danhua Zhu
BACKGROUND: Seed germination is important to soybean (Glycine max) growth and development, ultimately affecting soybean yield. A lower seed field emergence has been the main hindrance for breeding soybeans low in phytate. Although this reduction could be overcome by additional breeding and selection, the mechanisms of seed germination in different low phytate mutants remain unknown. In this study, we performed a comparative transcript analysis of two low phytate soybean mutants (TW-1 and TW-1-M), which have the same mutation, a 2 bp deletion in GmMIPS1, but show a significant difference in seed field emergence, TW-1-M was higher than that of TW-1 ...
January 18, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28098982/glycine-substitutions-in-collagen-heterotrimers-alter-triple-helical-assembly
#17
Katherine A Clements, Amanda M Acevedo-Jake, Douglas R Walker, Jeffrey D Hartgerink
Osteogenesis imperfecta typically results from missense mutations in the collagen genome where the required glycine residues are replaced with another amino acid. Many models have attempted to replicate the structure of mutated collagen on the triple helix level. However, composition and register control of the triple helix is complicated and requires extreme precision, especially when these destabilizing mutations are present. Here we present mutations to a composition- and register-controlled AAB helix where one of the requisite glycines in the A chain of the triple helix is changed to serine or alanine...
January 18, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28097768/biosynthesis-of-the-carbonylmethylene-structure-found-in-the-ketomemicin-class-of-pseudotripeptides
#18
Junpei Kawata, Taiki Naoe, Yasushi Ogasawara, Tohru Dairi
We recently discovered novel pseudotripeptides, the ketomemicins, which possess a C-terminal pseudodipeptide connected with a carbonylmethylene instead of an amide bond, through heterologous expression of gene clusters identified in actinobacteria. The carbonylmethylene structure is a stable isostere of the amide bond and its biological significance has been shown in several natural and synthetic products. Despite the biological importance of these compounds, little is known about how the carbonylmethylene structure is biosynthesized...
January 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28097750/the-structure-of-glycine-dihydrate-implications-for-the-crystallization-of-glycine-from-solution-and-its-structure-in-outer-space
#19
Wenqian Xu, Qiang Zhu, Chunhua Tony Hu
Glycine, the simplest amino acid, is also the most polymorphous. Herein, we report the structure determination of a long unknown phase of glycine, which was first reported by Pyne and Suryanarayanan in 2001. To date, this phase has only been prepared at 208 K as nanocrystals within ice. Through computational crystal-structure prediction and powder X-ray diffraction methods, we identified this elusive phase as glycine dihydrate (GDH), representing the first report on the structure of a hydrated glycine structure...
January 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28097715/c-di-amp-modulates-listeria-monocytogenes-central-metabolism-to-regulate-growth-antibiotic-resistance-and-osmoregulation
#20
Aaron T Whiteley, Nicholas E Garelis, Bret N Peterson, Philip H Choi, Liang Tong, Joshua J Woodward, Daniel A Portnoy
Cyclic di-adenosine monophosphate (c-di-AMP) is a conserved nucleotide second messenger critical for bacterial growth and resistance to cell wall-active antibiotics. In Listeria monocytogenes, the sole diadenylate cyclase, DacA, is essential in rich, but not synthetic media and ΔdacA mutants are highly sensitive to the β-lactam antibiotic cefuroxime. In this study, loss of function mutations in the oligopeptide importer (oppABCDF) and glycine betaine importer (gbuABC) allowed ΔdacA mutants to grow in rich medium...
January 18, 2017: Molecular Microbiology
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