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https://www.readbyqxmd.com/read/28340331/sumo-on-crmps-wrestling-for-pain
#1
Anne Baumann, Petri Kursula
No abstract text is available yet for this article.
March 24, 2017: Channels
https://www.readbyqxmd.com/read/28340002/overexpression-of-the-rice-sumo-e3-ligase-gene-ossiz1-in-cotton-enhances-drought-and-heat-tolerance-and-substantially-improves-fiber-yields-in-the-field-under-reduced-irrigation-and-rainfed-conditions
#2
Neelam Mishra, Li Sun, Xunlu Zhu, Jennifer Smith, Anurag Prakash Srivastava, Xiaojie Yang, Necla Pehlivan, Nardana Esmaeili, Hong Luo, Guoxin Shen, Don Jones, Dick Auld, John Burke, Paxton Payton, Hong Zhang
The Arabidopsis SUMO E3 ligase gene AtSIZ1 plays important roles in plant response to abiotic stresses as loss of function in AtSIZ1 leads to increased sensitivity to drought, heat and salt stresses. Overexpression of the AtSIZ1 rice homolog, OsSIZ1, leads to increased heat and drought tolerance in bentgrass, suggesting that the function of the E3 ligase SIZ1 is highly conserved in plants and it plays a critical role in abiotic stress responses. To test the possibility that the SUMO E3 ligase could be used to engineer drought- and heat-tolerant crops, the rice gene OsSIZ1 was overexpressed in cotton...
March 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28330929/the-role-of-pias-sumo-e3-ligases-in-cancer
#3
REVIEW
Andrea Rabellino, Cristina Andreani, Pier Paolo Scaglioni
SUMOylation modifies the interactome, localization, activity, and lifespan of its target proteins. This process regulates several cellular machineries, including transcription, DNA damage repair, cell-cycle progression, and apoptosis. Accordingly, SUMOylation is critical in maintaining cellular homeostasis, and its deregulation leads to the corruption of a plethora of cellular processes that contribute to disease states. Among the proteins involved in SUMOylation, the protein inhibitor of activated STAT (PIAS) E3-ligases were initially described as transcriptional coregulators...
March 22, 2017: Cancer Research
https://www.readbyqxmd.com/read/28326729/-study-of-human-leucine-rich-amelogenin-peptide-and-its-regulation-of-mineralization-by-cryogenic-transmission-electron-microscopy
#4
Tian Kun, Feng Xiaoyun, Du Qin, Liao Chuhang, Ren Xiaohua
OBJECTIVE: Recombinant human leucine-rich amelogenin peptide (LRAP) was studied by cryogenic transmission electron microscopy (TEM); evaluation focused on its self-assembly and crystal growth in vitro. METHODS: Human LRAP was recombined through prokaryotic expression vector pCold-SUMO and transformed into Escherichia coli BL21plys to acquire purified proteins. Cryogen TEM recorded assembly and self-assembling of LRAP from pH 3.5 to pH 8.0, and the hydroxyapatite crystal growth in the mixture of LRAP protein solution and artificial saliva was observed using TEM and selected area electron diffraction...
February 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28323006/the-role-of-sumoylation-in-cerebral-hypoxia-and-ischemia
#5
REVIEW
Myriam Peters, Betty Wielsch, Johannes Boltze
The process of protein modification by adding or detaching small ubiquitin-like modifiers (SUMO) proteins, called SUMOylation, contributes to the regulation of numerous processes in eukaryotic cells. SUMOylation also represents a key response and adaption mechanism to different forms of metabolic stress. The central nervous system (CNS) and neurons in particular are highly susceptible to hypoxic-ischemic stress due to the lack of significant oxygen and energy reserves. SUMOylation is observed in many molecular responses to metabolic stress in the brain, and is therefore supposed to represent an endogenous neuroprotective mechanism...
March 17, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28318435/determining-zebrafish-epitope-reactivity-to-commercially-available-antibodies
#6
Michael A Villarreal, Nicole M Biediger, Natalie A Bonner, Jennifer N Miller, Samantha K Zepeda, Benjamin J Ricard, Dana M García, Karen A Lewis
Antibodies raised against mammalian proteins may exhibit cross-reactivity with zebrafish proteins, making these antibodies useful for fish studies. However, zebrafish may express multiple paralogues of similar sequence and size, making them difficult to distinguish by traditional Western blot analysis. To identify the zebrafish proteins that are recognized by an antimammalian antibody, we developed a system to screen putative epitopes by cloning the sequences between the yeast SUMO protein and a C-terminal 6xHis tag...
March 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28318385/arsenic-induced-sumoylation-of-mus81-is-involved-in-regulating-genomic-stability
#7
Liyan Hu, Feikun Yang, Lou Lu, Wei Dai
Chronic environmental exposure to metal toxicants such as chromium and arsenic is closely related to the development of several types of common cancers. Genetic and epigenetic studies in the past decade reveal that post-translational modifications of histones play a role in metal carcinogenesis. However, exact molecular mechanisms of metal carcinogenesis remain to be elucidated. In this study we found that As2O3, an environmental metal toxicant, up-regulated overall modifications of many cellular proteins by SUMO2/3...
March 20, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28315713/maternal-senp7-programs-meiosis-architecture-and-embryo-survival-in-mouse
#8
ChunJie Huang, Di Wu, XiaoFei Jiao, Faheem Ahmed Khan, ChengLiang Xiong, XiaoMing Liu, Jing Yang, TaiLang Yin, LiJun Huo
Understanding the mechanisms underlying abnormal egg production and pregnancy loss is significant for human fertility. SENP7, a SUMO poly-chain editing enzyme, has been regarded as a mitotic regulator of heterochromatin integrity and DNA repair. Herein, we report the roles of SENP7 in mammalian reproductive scenario. Mouse oocytes deficient in SENP7 experienced meiotic arrest at prophase I and metaphase I stages, causing substantial decrease of mature eggs. Hyperaceylation and hypomethylation of histone H3 and up-regulation of Cdc14B/C accompanied by down-regulation of CyclinB1 and CyclinB2 were further recognized as contributors to defective M-phase entry and spindle assembly in oocytes...
March 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28289178/proteome-wide-mapping-of-endogenous-sumoylation-sites-in-mouse-testis
#9
Lili Cai, Jun Tu, Lei Song, Zhihua Gao, Kai Li, Yunzhi Wang, Yang Liu, Fan Zhong, Rui Ge, Jun Qin, Chen Ding, Fuchu He
SUMOylation is a reversible post-translational modification involved in various critical biological processes. To date, there is limited approach for endogenous wild-type SUMO-modified peptides enrichment and SUMOylation sites identification. In this study, we generated a high-affinity SUMO1 antibody to facilitate the enrichment of endogenous SUMO1-modified peptides from Trypsin/Lys-C protease digestion. Following secondary Glu-C protease digestion, we identified 53 high-confidence SUMO1-modified sites from mouse testis by using high-resolution mass spectrometry...
March 13, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28285006/gata5-sumoylation-is-indispensable-for-zebrafish-cardiac-development
#10
Bin Wen, Hao Yuan, Xiaohui Liu, Haihong Wang, Saijuan Chen, Zhu Chen, Hugues de The, Jun Zhou, Jun Zhu
BACKGROUND: SUMOylation is a critical regulatory protein modification in eukaryotic cells and plays a pivotal role in cardiac development and disease. Several cardiac transcription factors are modified by SUMO, but little is known about the impact of SUMOylation on their function during cardiac development. METHODS: We used a zebrafish model to address the impact of SUMOylation on GATA5, an essential transcription factor in zebrafish cardiac development. GATA5 SUMOylation was probed by western blot, the subcellular localization and transcriptional activity of GATA5 mutants were examined by immunostaining and luciferase reporter assay...
March 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28284030/sumo-conjugation-a-mechanistic-view
#11
REVIEW
Andrea Pichler, Chronis Fatouros, Heekyoung Lee, Nathalie Eisenhardt
The regulation of protein fate by modification with the small ubiquitin-related modifier (SUMO) plays an essential and crucial role in most cellular pathways. Sumoylation is highly dynamic due to the opposing activities of SUMO conjugation and SUMO deconjugation. SUMO conjugation is performed by the hierarchical action of E1, E2 and E3 enzymes, while its deconjugation involves SUMO-specific proteases. In this review, we summarize and compare the mechanistic principles of how SUMO gets conjugated to its substrate...
March 1, 2017: Biomolecular Concepts
https://www.readbyqxmd.com/read/28282126/tuning-the-rectification-ratio-by-changing-the-electronic-nature-open-shell-and-closed-shell-in-donor-acceptor-self-assembled-monolayers
#12
Manuel Souto, Li Yuan, Dayana C Morales, Li Jiang, Imma Ratera, Christian A Nijhuis, Jaume Veciana
This Communication describes the mechanism of charge transport across self-assembled monolayers (SAMs) of two donor-acceptor systems consisting of a polychlorotriphenylmethyl (PTM) electron-acceptor moiety linked to an electron-donor ferrocene (Fc) unit supported by ultraflat template-stripped Au and contacted by a eutectic alloy of gallium and indium top contacts. The electronic and supramolecular structures of these SAMs were well characterized. The PTM unit can be switched between the nonradical and radical forms, which influences the rectification behavior of the junction...
March 16, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28278057/anabolic-transcription-secrets-of-the-sumo-diet
#13
Jorrit Enserink, Aurélie Nguéa P, Pierre Chymkowitch
No abstract text is available yet for this article.
February 14, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28277940/a-single-structurally-conserved-sumoylation-site-in-crmp2-controls-nav1-7-function
#14
Erik Thomas Dustrude, Samantha Perez-Miller, Liberty François-Moutal, Aubin Moutal, May Khanna, Rajesh Khanna
The neuronal collapsin response mediator protein 2 (CRMP2) undergoes several posttranslational modifications that codify its functions. Most recently, CRMP2 SUMOylation (addition of small ubiquitin like modifier (SUMO)) was identified as a key regulatory step within a modification program that codes for CRMP2 interaction with, and trafficking of, voltage-gated sodium channel NaV1.7. In this paper, we illustrate the utility of combining sequence alignment within protein families with structural analysis to identify, from several putative SUMOylation sites, those that are most likely to be biologically relevant...
February 28, 2017: Channels
https://www.readbyqxmd.com/read/28275011/ataxin-3-consolidates-the-mdc1-dependent-dna-double-strand-break-response-by-counteracting-the-sumo-targeted-ubiquitin-ligase-rnf4
#15
Annika Pfeiffer, Martijn S Luijsterburg, Klara Acs, Wouter W Wiegant, Angela Helfricht, Laura K Herzog, Melania Minoia, Claudia Böttcher, Florian A Salomons, Haico van Attikum, Nico P Dantuma
The SUMO-targeted ubiquitin ligase RNF4 functions at the crossroads of the SUMO and ubiquitin systems. Here, we report that the deubiquitylation enzyme (DUB) ataxin-3 counteracts RNF4 activity during the DNA double-strand break (DSB) response. We find that ataxin-3 negatively regulates ubiquitylation of the checkpoint mediator MDC1, a known RNF4 substrate. Loss of ataxin-3 markedly decreases the chromatin dwell time of MDC1 at DSBs, which can be fully reversed by co-depletion of RNF4. Ataxin-3 is recruited to DSBs in a SUMOylation-dependent fashion, and in vitro it directly interacts with and is stimulated by recombinant SUMO, defining a SUMO-dependent mechanism for DUB activity toward MDC1...
March 8, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28267405/genetic-characterization-of-t-dna-insertions-in-the-genome-of-the-arabidopsis-thaliana-sumo1-2-knock-down-line
#16
Valentin Hammoudi, Georgios Vlachakis, Ronnie de Jonge, Timo M Breit, Harrold A van den Burg
Sumoylation is an essential post-translational modification in Arabidopsis thaliana, which entails the conjugation of the SUMO protein onto lysine residues in target proteins. In Arabidopsis, 2 closely related genes, SUMO1 and SUMO2, act redundantly and are in combination essential for plant development, i.e. the combined loss of SUMO1 and SUMO2 results in embryo-lethality. To circumvent this lethality, SUMO2 was previously knocked down in a sumo1 knockout background by expressing an artificial microRNA that targets SUMO2 (amiR-SUMO2)...
March 4, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28265077/arsenic-trioxide-targets-mthfd1-and-sumo-dependent-nuclear-de-novo-thymidylate-biosynthesis
#17
Elena Kamynina, Erica R Lachenauer, Aislyn C DiRisio, Rebecca P Liebenthal, Martha S Field, Patrick J Stover
Arsenic exposure increases risk for cancers and is teratogenic in animal models. Here we demonstrate that small ubiquitin-like modifier (SUMO)- and folate-dependent nuclear de novo thymidylate (dTMP) biosynthesis is a sensitive target of arsenic trioxide (As2O3), leading to uracil misincorporation into DNA and genome instability. Methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) and serine hydroxymethyltransferase (SHMT) generate 5,10-methylenetetrahydrofolate for de novo dTMP biosynthesis and translocate to the nucleus during S-phase, where they form a multienzyme complex with thymidylate synthase (TYMS) and dihydrofolate reductase (DHFR), as well as the components of the DNA replication machinery...
March 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28264436/toxin-fused-with-sumo-tag-a-new-expression-vector-strategy-to-obtain-recombinant-venom-toxins-with-easy-tag-removal-inside-the-bacteria
#18
Lhiri H A L Shimokawa-Falcão, Maria C Caporrino, Katia C Barbaro, Maisa S Della-Casa, Geraldo S Magalhães
Many animal toxins may target the same molecules that need to be controlled in certain pathologies; therefore, some toxins have led to the formulation of drugs that are presently used, and many other drugs are still under development. Nevertheless, collecting sufficient toxins from the original source might be a limiting factor in studying their biological activities. Thus, molecular biology techniques have been applied in order to obtain large amounts of recombinant toxins into Escherichia coli. However, most animal toxins are difficult to express in this system, which results in insoluble, misfolded, or unstable proteins...
February 27, 2017: Toxins
https://www.readbyqxmd.com/read/28262828/senp3-mediated-desumoylation-of-drp1-facilitates-interaction-with-mff-to-promote-cell-death
#19
Chun Guo, Kevin A Wilkinson, Ashley J Evans, Philip P Rubin, Jeremy M Henley
The GTPase dynamin-related protein 1 (Drp1) is essential for physiological and pathophysiological mitochondrial fission. DeSUMOylation of Drp1 by the enzyme SENP3 promotes cell death during reperfusion after ischaemia by enhancing Drp1 partitioning to the mitochondrial outer membrane (MOM), which causes cytochrome c release and apoptosis. However, how deSUMOylation recruits Drp1 to the MOM is unknown. Here we show that deSUMOylation selectively promotes Drp1 binding to the MOM resident adaptor protein mitochondrial fission factor (Mff)...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28258134/senp1-promotes-hypoxia-induced-cancer-stemness-by-hif-1%C3%AE-desumoylation-and-senp1-hif-1%C3%AE-positive-feedback-loop
#20
Chun-Ping Cui, Carmen Chak-Lui Wong, Alan Ka-Lun Kai, Daniel Wai-Hung Ho, Eunice Yuen-Ting Lau, Yu-Man Tsui, Lo-Kong Chan, Tan-To Cheung, Kenneth Siu-Ho Chok, Albert C Y Chan, Regina Cheuk-Lam Lo, Joyce Man-Fong Lee, Terence Kin-Wah Lee, Irene Oi Lin Ng
OBJECTIVE: We investigated the effect and mechanism of hypoxic microenvironment and hypoxia-inducible factors (HIFs) on hepatocellular carcinoma (HCC) cancer stemness. DESIGN: HCC cancer stemness was analysed by self-renewal ability, chemoresistance, expression of stemness-related genes and cancer stem cell (CSC) marker-positive cell population. Specific small ubiquitin-like modifier (SUMO) proteases 1 (SENP1) mRNA level was examined with quantitative PCR in human paired HCCs...
March 3, 2017: Gut
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