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https://www.readbyqxmd.com/read/29330559/a-post-translational-balancing-act-the-good-and-the-bad-of-sumoylation-in-pancreatic-islets
#1
EDITORIAL
Patrick E MacDonald
Post-translational modification of proteins contributes to the control of cell function and survival. The balance of these in insulin-producing pancreatic beta cells is important for the maintenance of glucose homeostasis. Protection from the damaging effects of reactive oxygen species is required for beta cell survival, but if this happens at the expense of insulin secretory function then the ability of islets to respond to changing metabolic conditions may be compromised. In this issue of Diabetologia, He et al ( https://doi...
January 12, 2018: Diabetologia
https://www.readbyqxmd.com/read/29328071/sumoylation-of-histone-deacetylase-1-regulates-myod-signaling-during-myogenesis
#2
Hosouk Joung, Sehee Kwon, Kyoung-Hoon Kim, Yun-Gyeong Lee, Sera Shin, Duk-Hwa Kwon, Yeong-Un Lee, Taewon Kook, Nakwon Choe, Jeong Chul Kim, Young-Kook Kim, Gwang Hyeon Eom, Hyun Kook
Sumoylation, the conjugation of a small ubiquitin-like modifier (SUMO) protein to a target, has diverse cellular effects. However, the functional roles of the SUMO modification during myogenesis have not been fully elucidated. Here, we report that basal sumoylation of histone deacetylase 1 (HDAC1) enhances the deacetylation of MyoD in undifferentiated myoblasts, whereas further sumoylation of HDAC1 contributes to switching its binding partners from MyoD to Rb to induce myocyte differentiation. Differentiation in C2C12 skeletal myoblasts induced new immunoblot bands above HDAC1 that were gradually enhanced during differentiation...
January 12, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29326161/sumo2-3-modification-of-activating-transcription-factor-5-atf5-controls-its-dynamic-translocation-at-the-centrosome
#3
Yunsheng Yuan, Kari Gaither, Eugene Kim, Edward Liu, Ming Hu, Kathy Lengel, Dongmeng Qian, Yidi Xu, Bin Wang, Henning Knipprath, David Liu
Activating transcription factor 5 (ATF5) is a member of the ATF/CREB family of transcription factors. ATF5 regulates stress responses and cell survival, proliferation, and differentiation and also plays a role in viral infections, cancer, diabetes, schizophrenia, and the olfactory system. Moreover, it was found to also have a critical, cell cycle-dependent structural function at the centrosome. However, the mechanism that controls ATF5's localization at the centrosome is unclear. Here, we report that ATF5 is SUMO2/3- modified at a conserved SUMO-targeting consensus site in various types of mammalian cells...
January 11, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29325714/aging-related-sumoylation-pattern-in-the-cortex-and-blood-plasma-of-wild-type-mice
#4
E Ficulle, M D Shah Sufian, C Tinelli, M Corbo, M Feligioni
Protein activities and mechanisms related to aging has become a growing interest nowadays. Since SUMOylation is implicated in several cellular processes, its investigation related to senescence, aging and frailty is of high interest. In our study, wild type mice cortical lysates, synaptosomes and plasma have been processed to evaluate SUMOylation and SUMO machinery expression (Ubc9 and SENP1 enzymes) profile at different ages. In cortical lysates, SUMO-1ylation reached a peak at 6 months followed by a decrease; while in synaptosomes, it progressively increased till 18 months...
January 8, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29324855/ctls-a-new-class-of-ring-h2-ubiquitin-ligases-uncovered-by-yeell-a-motif-close-to-the-ring-domain-that-is-present-across-eukaryotes
#5
Domingo Jiménez-López, Laura Aguilar-Henonin, Juan Manuel González-Prieto, Victor Aguilar-Hernández, Plinio Guzmán
RING ubiquitin E3 ligases enclose a RING domain for ubiquitin ligase activity and associated domains and/or conserved motifs outside the RING domain that collectively facilitate their classification and usually reveal some of key information related to mechanism of action. Here we describe a new family of E3 ligases that encodes a RING-H2 domain related in sequence to the ATL and BTL RING-H2 domains. This family, named CTL, encodes a motif designed as YEELL that expands 21 amino acids next to the RING-H2 domain that is present across most eukaryotic lineages...
2018: PloS One
https://www.readbyqxmd.com/read/29305585/sumoylation-of-notch1-represses-its-target-gene-expression-during-cell-stress
#6
Christian J M Antila, Vilma Rraklli, Henri A Blomster, Käthe M Dahlström, Tiina A Salminen, Johan Holmberg, Lea Sistonen, Cecilia Sahlgren
The Notch signaling pathway is a key regulator of stem cells during development, and its deregulated activity is linked to developmental defects and cancer. Transcriptional activation of Notch target genes requires cleavage of the Notch receptor in response to ligand binding, production of the Notch intracellular domain (NICD1), NICD1 migration into the nucleus, and assembly of a transcriptional complex. Post-translational modifications of Notch regulate its trafficking, turnover, and transcriptional activity...
January 5, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29299635/both-conditional-ablation-and-overexpression-of-e2-sumo-conjugating-enzyme-ubc9-in-mouse-pancreatic-beta-cells-result-in-impaired-beta-cell-function
#7
Xiaoyu He, Qiaohong Lai, Cai Chen, Na Li, Fei Sun, Wenting Huang, Shu Zhang, Qilin Yu, Ping Yang, Fei Xiong, Zhishui Chen, Quan Gong, Boxu Ren, Jianping Weng, Décio L Eizirik, Zhiguang Zhou, Cong-Yi Wang
AIMS/HYPOTHESIS: Post-translational attachment of a small ubiquitin-like modifier (SUMO) to the lysine (K) residue(s) of target proteins (SUMOylation) is an evolutionary conserved regulatory mechanism. This modification has previously been demonstrated to be implicated in the control of a remarkably versatile regulatory mechanism of cellular processes. However, the exact regulatory role and biological actions of the E2 SUMO-conjugating enzyme (UBC9)-mediated SUMOylation function in pancreatic beta cells has remained elusive...
January 3, 2018: Diabetologia
https://www.readbyqxmd.com/read/29277914/synaptic-localization-of-the-sumoylation-regulating-protease-senp5-in-the-adult-mouse-brain
#8
Hiroki Akiyama, Kazuhiko Nakadate, Shin-Ichi Sakakibara
Covalent conjugation of small ubiquitin-like modifiers (SUMOs) or SUMOylation is a reversible post-translational modification that regulates the stability and function of target proteins. SUMOs are removed from substrate proteins by sentrin/SUMO-specific proteases (SENPs). Numerous studies have implicated SUMOylation in various physiological and pathological processes in neurons. To understand the functional roles of SUMOylation, it is necessary to determine the distribution of enzymes regulating SUMO conjugation and deconjugation; yet, the localization of SENPs has not been described in detail in intact brain tissue...
December 26, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29276714/radiological-changes-in-the-cervical-spine-in-freshman-collegiate-sumo-wrestlers
#9
Yasuaki Nakagawa, Shogo Mukai, Kazufumi Minami, Yuji Hattori, Takashi Nakamura
Background: Sumo has long been a traditional sport in Japan and is rapidly attracting enthusiasts abroad. Sumo wrestling entails a risk of impact to the cervical spine during an initial charge. Few reports are available in the English-language literature regarding radiological changes in the cervical spine in sumo wrestlers. Purpose: To examine radiological changes in the cervical spine in freshmen collegiate sumo wrestlers. Study Design: Case series; Level of evidence, 4...
December 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29275599/-hypoxia-inducible-factor-1%C3%AE-desumoylation-reduces-the-stemness-maintenance-ability-of-endometrial-cancer-stem-cell-and-increases-its-chemosensitivity
#10
L Yuan, Z M Jiang, X H Chen, X Y Bian, Y X Li, X F Ma, X Z Liu
Objective: To reduce the stemness maintenance ability of endometrial cancer stem cell and increase its sensitivity to chemotherapy by inducing hypoxia inducible factor 1α (HIF-1α) protein deSUMOylation. Methods: Lentiviral plasmid mediated ubiquitin carrier protein 9 (Ubc9) gene silencing was transgened into KLE endometrial carcinoma cells. The expression of Ubc9, small ubiquitin-related modifier 1(SUMO1) and HIF-1α protein was detected by Western blotting. Then tumor stem cells clones were cultured in 96 well plates, and these clone balls diameter were calculated...
December 5, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29273057/cenp-b-protects-centromere-chromatin-integrity-by-facilitating-histone-deposition-via-the-h3-3-specific-chaperone-daxx
#11
Viacheslav M Morozov, Serena Giovinazzi, Alexander M Ishov
BACKGROUND: The main chromatin unit, the nucleosome, can be modulated by the incorporation of histone variants that, in combination with posttranslational histones modifications, determine epigenetics properties of chromatin. Understanding the mechanism that creates a histone variants landscape at different genomic elements is expected to elevate our comprehension of chromatin assembly and function. The Daxx chaperone deposits transcription-associated histone H3.3 at centromeres, but mechanism of centromere-specific Daxx targeting remains unclear...
December 22, 2017: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/29250092/arabidopsis-tcp-transcription-factors-interact-with-the-sumo-conjugating-machinery-in-nuclear-foci
#12
Magdalena J Mazur, Benjamin J Spears, André Djajasaputra, Michelle van der Gragt, Georgios Vlachakis, Bas Beerens, Walter Gassmann, Harrold A van den Burg
In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs) are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybrid library containing more than 1,100 TFs. We identified 76 interactors that foremost interact with the SUMO conjugation enzyme SCE1 and/or the SUMO E3 ligase SIZ1. These interactors belong to various TF families, which control a wide range of processes in plant development and stress signaling...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29246538/sumoylation-and-ubiquitylation-crosstalk-in-the-control-of-%C3%AE-np63%C3%AE-protein-stability
#13
Michela Ranieri, Maria Vivo, Marco De Simone, Luisa Guerrini, Alessandra Pollice, Girolama La Mantia, Viola Calabrò
ΔNp63α is finely and strictly regulated during embryogenesis and differentiation. ΔNp63α is the only p63 isoform degraded by the proteasome after Ubiquitin and SUMO (Small Ubiquitin-like MOdifier) conjugation. Here, we show that p63 ubiquitylation per se is not the signal triggering p63 proteasomal degradation. Taking advantage of natural ΔNp63α mutants isolated by patients with Split Hand and Foot Malformation IV syndrome, we found that SUMO and Ub modifications are not redundant and both are required to guarantee efficient ΔNp63α degradation...
December 12, 2017: Gene
https://www.readbyqxmd.com/read/29244762/towards-realistic-urban-traffic-experiments-using-dfrouter-heuristic-validation-and-extensions
#14
Jorge Luis Zambrano-Martinez, Carlos T Calafate, David Soler, Juan-Carlos Cano
Traffic congestion is an important problem faced by Intelligent Transportation Systems (ITS), requiring models that allow predicting the impact of different solutions on urban traffic flow. Such an approach typically requires the use of simulations, which should be as realistic as possible. However, achieving high degrees of realism can be complex when the actual traffic patterns, defined through an Origin/Destination (O-D) matrix for the vehicles in a city, remain unknown. Thus, the main contribution of this paper is a heuristic for improving traffic congestion modeling...
December 15, 2017: Sensors
https://www.readbyqxmd.com/read/29242151/resveratrol-induces-sumoylated-cox-2-dependent-anti-proliferation-in-human-prostate-cancer-lncap-cells
#15
Tsai-Mu Cheng, Yu-Tang Chin, Yih Ho, Yi-Ru Chen, Yung-Ning Yang, Yu-Chen Yang, Ya-Jang Shih, Ting-I Lin, Hung-Yun Lin, Paul J Davis
Cyclooxygenase (COX)-2 has been implicated in cancer development. However, resveratrol-induced nuclear accumulation of COX-2 enhances p53-dependent anti-proliferation in different types of cancers. Treatment with resveratrol leads to phosphorylation and nuclear translocation of mitogen-activated protein kinase (ERK1/2), and accumulation of nuclear COX-2 to complex with pERK1/2 and p53. The consequence is Ser-15 phosphorylation of p53 (pSer15-p53), and induction of anti-proliferation in cancer cells. We investigated the mechanisms by which resveratrol-inducible COX-2 facilitates p53-dependent anti-proliferation in prostate cancer LNCaP cells...
December 11, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29236778/interaction-of-porcine-reproductive-and-respiratory-syndrome-virus-proteins-with-sumo-conjugating-enzyme-reveals-the-sumoylation-of-nucleocapsid-protein
#16
Cong Wang, Nanfang Zeng, Siyu Liu, Qi Miao, Lei Zhou, Xinna Ge, Jun Han, Xin Guo, Hanchun Yang
SUMOylation is a reversible post-translational modification that regulates the function of target protein. In this study, we first predicted by software that the multiple proteins of porcine reproductive and respiratory syndrome virus (PRRSV) could be sumoylated. Next, we confirmed that Nsp1β, Nsp4, Nsp9, Nsp10 and nucleocapsid (N) protein of PRRSV could interact with the sole SUMO E2 conjugating enzyme Ubc9, and Ubc9 could be co-localized with Nsp1β, Nsp4, Nsp9 and Nsp10 in the cytoplasm, while with N protein in both the cytoplasm and nucleus...
2017: PloS One
https://www.readbyqxmd.com/read/29234079/a-genetic-screen-to-discover-sumoylated-proteins-in-living-mammalian-cells
#17
Maki Komiya, Akihiro Ito, Mizuki Endo, Daisuke Hiruma, Mitsuru Hattori, Hisato Saitoh, Minoru Yoshida, Takeaki Ozawa
Post-translational modification by the Small Ubiquitin-related Modifier (SUMO) is indispensable for diverse biological mechanisms. Although various attempts have been made to discover novel SUMO substrate proteins to unveil the roles of SUMOylation, the reversibility of SUMOylation, and the differences in the SUMOylation level still makes it difficult to explore infrequently-SUMOylated proteins in mammalian cells. Here, we developed a method to screen for mammalian SUMOylated proteins using the reconstitution of split fluorescent protein fragments in living mammalian cells...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29234076/molecular-mechanism-of-k65-acetylation-induced-attenuation-of-ubc9-and-the-ndsm-interaction
#18
Mandar T Naik, Mooseok Kang, Chun-Chen Ho, Pei-Hsin Liao, Yung-Lin Hsieh, Nandita M Naik, Szu-Huan Wang, Iksoo Chang, Hsiu-Ming Shih, Tai-Huang Huang
The negatively charged amino acid-dependent sumoylation motif (NDSM) carries an additional stretch of acidic residues downstream of the consensus Ψ-K-x-E/D sumoylation motif. We have previously shown that acetylation of the SUMO E2 conjugase enzyme, Ubc9, at K65 downregulates its binding to the NDSM and renders a selective decrease in sumoylation of substrates with the NDSM motif. Here, we provide detailed structural, thermodynamic, and kinetics results of the interactions between Ubc9 and its K65 acetylated variant (Ac-Ubc9K65) with three NDSMs derived from Elk1, CBP, and Calpain2 to rationalize the mechanism beneath this reduced binding...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29234018/dynamics-of-rif1-sumoylation-is-regulated-by-pias4-in-the-maintenance-of-genomic-stability
#19
Ramesh Kumar, Cheok Chit Fang
RIF1 plays a key role in inhibiting DNA end resection and promoting NHEJ mediated DNA double stand break repair in G1. However, whether SUMOlyation may regulate RIF1 functions is still largely unknown. Here, we report that RIF1 is SUMOlyated in response to DNA damage. We identified PIAS4 as the primary SUMO E3 ligase required for the SUMOylation of RIF1 protein. Mammalian cells compromised of PIAS4 expression, show impaired RIF1 SUMOylation and defective for the disassembly of DNA damage responsive RIF1 foci...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29231927/the-sumo-guards-for-snail
#20
COMMENT
Xin Ye, Robert A Weinberg
No abstract text is available yet for this article.
November 17, 2017: Oncotarget
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