keyword
MENU ▼
Read by QxMD icon Read
search

sumo

keyword
https://www.readbyqxmd.com/read/28817247/assessing-predictions-of-fitness-effects-of-missense-mutations-in-sumo-conjugating-enzyme-ube2i
#1
Jing Zhang, Lisa N Kinch, Qian Cong, Jochen Weile, Song Sun, Atina G Cote, Frederick P Roth, Nick V Grishin
The exponential growth of genomic variants uncovered by next-generation sequencing necessitates efficient and accurate computational analyses to predict their functional effects. A number of computational methods have been developed for the task, but few unbiased comparisons of their performance are available. To fill the gap, The Critical Assessment of Genome Interpretation (CAGI) comprehensively assesses phenotypic predictions on newly collected experimental datasets. Here, we present the results of the SUMO conjugase challenge where participants were predicting functional effects of missense mutations in human SUMO-conjugating enzyme UBE2I...
September 2017: Human Mutation
https://www.readbyqxmd.com/read/28796315/senp1-hif-1%C3%AE-feedback-loop-modulates-hypoxia-induced-cell-proliferation-invasion-and-emt-in-human-osteosarcoma-cells
#2
Xiaowei Wang, Xiaoju Liang, Huan Liang, Bing Wang
Hypoxia is an element intrinsic to most solid-tumor microenvironments, including that of osteosarcoma (OS), and is associated with resistance to therapy, poor survival, and a malignant phenotype. The purpose of the present study was to investigate the role and underlying mechanism of SUMO-specific protease 1 (SENP1)/hypoxia-inducible factor-1α (HIF-1α) feedback loop in hypoxic microenvironment of OS. We observed that the expression of SENP1 was remarkably upregulated in OS cells. Additionally, there was a concomitant high expression of HIF-1α and SENP1 in MG-63 cells under a hypoxic microenvironment...
August 10, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28792214/aggregation-and-fibril-structure-of-a%C3%AE-m01-42-and-a%C3%AE-1-42
#3
Robert Silvers, Michael T Colvin, Kendra K Frederick, Angela C Jacavone, Susan L Lindquist, Sara Linse, Robert G Griffin
A mechanistic understanding of Aβ aggregation and high-resolution structures of Aβ fibrils and oligomers are vital to elucidating relevant details of neurodegeneration in Alzheimer's disease, which will facilitate the rational design of diagnostic and therapeutic protocols. The most detailed and reproducible insights into the structure and kinetics of have been achieved using recombinant Aβ peptides produced by direct expression, which results in the addition of a methionine on the N-terminus. While the length of the C-terminus is well established to have a profound impact on the peptide's aggregation propensity, structure, and neurotoxicity, the impact of the N-terminal methionine on the aggregation pathways, and structure is unclear...
August 9, 2017: Biochemistry
https://www.readbyqxmd.com/read/28789962/expression-and-characterization-of-an-enhanced-recombinant-heparinase-i-with-chitin-binding-domain
#4
Shuqin Xu, Meiling Qiu, Xuanyue Zhang, Jinghua Chen
Heparinase I (Hep I) can efficiently depolymerize heparin and heparin sulfate to oligosaccharides or unsaturated disaccharides, which resulted in loss of physiological function such as blood coagulation. In order to realize the immobilization of Hep I on chitin carriers, we cloned Hep I with the chitin binding domain (ChBD) as a chitin-affinity tag, and the Small Ubiquitin-like MOdifier (SUMO) linker as a solvation enhancer in different fusion sequence. DNA and protein gels suggested that 4 kinds of recombinants were successfully constructed and expressed in Escherichia coli (E...
August 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28782654/mir-485-5p-modulates-mitochondrial-fission-through-targeting-mitochondrial-anchored-protein-ligase-in-cardiac-hypertrophy
#5
Yanfang Zhao, Murugavel Ponnusamy, Cuiyun Liu, Jing Tian, Yanhan Dong, Jinning Gao, Chaoqun Wang, Yuan Zhang, Lei Zhang, Kun Wang, Peifeng Li
The pathogenesis of cardiac hypertrophy is tightly associated with mitochondrial dysfunction. Disequilibrium of mitochondrial dynamic is one of the main drivers in the pathological processes during development of various cardiac diseases. However, the effect of mitochondrial dynamics on cardiac hypertrophy remains largely unclear. MicroRNAs (miRNAs) are small noncoding RNAs that can switch off expression of many genes. Mitochondrial anchored protein ligase (MAPL) is a small ubiquitin-like modifier (SUMO) E3 ligase, which is an important contributor in mitochondrial fission process...
August 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28770955/the-regulatory-role-of-dopamine-receptor-d1-on-pp2a-via-sumo-1-modification
#6
C-Q Yu, L-Q Yin, Z-T Tu, D-W Liu, W-P Luo
OBJECTIVE: Renal dopamine receptor D1 played a critical role in the regulation of body blood pressure. Under hypertension, over-phosphorylation of D1 receptor impaired its function. G protein kinase 4 (GRK4) and protein phosphatase 2A (PP2A) exerted the effect to phosphorylate and de-phosphorylate D1 receptor. A current study revealed that the inhibition of GRK4 cannot normalize the phosphorylation level of D1 receptor. Meanwhile, the PP2A was activated under hypertension, indicating abnormal de-phosphorylation function of D1 receptor, the reason for which remains unknown...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28770041/stromal-senp1-promotes-mouse-early-folliculogenesis-by-regulating-bmp4-expression
#7
Shu Tan, Boya Feng, Mingzhu Yin, Huanjiao Jenny Zhou, Ge Lou, Weidong Ji, Yonghao Li, Wang Min
BACKGROUND: Mammalian folliculogenesis, maturation of the ovarian follicles, require both growth factors derived from oocyte and surrounding cells, including stromal cells. However, the mechanism by which stromal cells and derived factors regulate oocyte development remains unclear. RESULTS: We observed that SENP1, a small ubiquitin-related modifier (SUMO)-specific isopeptidase, was expressed in sm22α-positive stromal cells of mouse ovary. The sm22α-positive stromal cells tightly associated with follicle maturation...
2017: Cell & Bioscience
https://www.readbyqxmd.com/read/28768860/zinc-finger-containing-cellular-transcription-corepressor-zbtb25-promotes-influenza-virus-rna-transcription-and-is-a-target-for-zinc-ejector-drugs
#8
Shu-Chuan Chen, King-Song Jeng, Michael M C Lai
Influenza A virus (IAV) replication relies on an intricate interaction between virus and host cells. How the cellular proteins are usurped for IAV replication remains largely obscure. The aim of this study was to search for novel and potential cellular factors that participate in IAV replication. ZBTB25, a transcription repressor of a variety of cellular genes, was identified by an RNAi genomic library screening. Depletion of ZBTB25 significantly reduced IAV production. Conversely, overexpression of ZBTB25 enhanced it...
August 2, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28760777/sumoylation-negatively-regulates-angiogenesis-by-targeting-endothelial-notch-signaling
#9
Xiaolong Zhu, Sha Ding, Cong Qiu, Yanna Shi, Lin Song, Yueyue Wang, Yuewen Wang, Jinying Li, Yiran Wang, Yi Sun, Lingfeng Qin, Jun Chen, Michael Simons, Wang Min, Luyang Yu
Rationale: The highly conserved NOTCH signaling pathway functions as a key cell-cell interaction mechanism controlling cell-fate and tissue patterning, while its dysregulation is implicated in a variety of developmental disorders and cancers. The pivotal role of endothelial NOTCH in regulation of angiogenesis is widely appreciated; however, little is known about what controls it signal transduction. Our previous study indicated the potential role of post-translational small ubiquitin-like modifier (SUMO) modification (SUMOylation) in vascular disorders...
July 31, 2017: Circulation Research
https://www.readbyqxmd.com/read/28757467/high-level-expression-and-purification-of-soluble-form-of-human-natural-killer-cell-receptor-nkr-p1-in-hek293s-gnti-cells
#10
Jan Bláha, Barbora Kalousková, Ondřej Skořepa, Samuel Pažický, Petr Novák, Ondřej Vaněk
Human natural killer receptor protein 1 (NKR-P1, CD161, gene klrb1) is a C-type lectin-like receptor of natural killer (NK) cells responsible for recognition of its cognate protein ligand lectin-like transcript 1 (LLT1). NKR-P1 is the single human orthologue of the prototypical rodent NKR-P1 receptors. Naturally, human NKR-P1 is expressed on the surface of NK cells, where it serves as inhibitory receptor; and on T and NKT cells functioning as co-stimulatory receptor promoting secretion of IFNγ. Most notably, it is expressed on Th17 and Tc17 lymphocytes where presumably promotes targeting into LLT1 expressing immunologically privileged niches...
July 27, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28748780/mir-133a-3p-targets-sumo-specific-protease-1-to-inhibit-cell-proliferation-and-cell-cycle-progress-in-colorectal-cancer
#11
Guo-Qiang Zhou, Fu Han, Zhi-Liang Shi, Liang Yu, Xue-Feng Li, Cheng Yu, Cheng-Long Shen, Dai-Wei Wan, Xin-Quo Zhu, Rui Li, Song-Bing He
Dysregulation of SUMO-specific protease 1(SENP1) expression has been reported in several kinds of cancer, including human colorectal and prostate cancers, proposing SENP1 as a oncogene with a critical role in cancer progression. MiR-133a-3p has been reported as a tumor suppressor in several malignant neoplasias. However, the precise molecular mechanisms underlying its role in colorectal cancer remain largely unknown. The aim of this work is to investigate the relationship between miR-133a-3p and SENP1 in colorectal cancer cells...
July 26, 2017: Oncology Research
https://www.readbyqxmd.com/read/28747610/retraction-protective-effect-of-senp3-mediated-sumoylation-against-cadmium-toxicity
#12
Hongquan Zhu
[This retracts the article DOI: 10.2131/jts.42.233.].
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28747609/increased-sumo-2-3-ylation-mediated-by-senp3-degradation-is-protective-against-cadmium-induced-caspase-3-dependent-cytotoxicity
#13
Jia Luo, Sonam Gurung, Laura Lee, Jeremy M Henley, Kevin A Wilkinson, Chun Guo
Increased post-translational modification of proteins by SUMO-2/3 is a cytoprotective response against cell stress induced by ischaemia and reperfusion. However, it is still unclear what other cell stressors trigger protein SUMOylation, what mechanisms enhance and maintain the enhanced SUMOylation, and if it is a general protective mediator against other cytotoxic stresses. Here, we show increased levels of SUMOylation and decreased levels of the SUMO deconjugating enzyme SENP3 in PC12 cells treated with the toxic heavy metal cadmium...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28743749/sumoylation-determines-the-voltage-required-to-activate-cardiac-iks-channels
#14
Dazhi Xiong, Tian Li, Hui Dai, Anthony F Arena, Leigh D Plant, Steve A N Goldstein
IKs channels open in response to depolarization of the membrane voltage during the cardiac action potential, passing potassium ions outward to repolarize ventricular myocytes and end each beat. Here, we show that the voltage required to activate IKs channels depends on their covalent modification by small ubiquitin-like modifier (SUMO) proteins. IKs channels are comprised of four KCNQ1 pore-forming subunits, two KCNE1 accessory subunits, and up to four SUMOs, one on Lys424 of each KCNQ1 subunit. Each SUMO shifts the half-maximal activation voltage (V1/2) of IKs ∼ +8 mV, producing a maximal +34-mV shift in neonatal mouse cardiac myocytes or Chinese hamster ovary (CHO) cells expressing the mouse or human subunits...
July 25, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28740167/dna-end-resection-requires-constitutive-sumoylation-of-ctip-by-cbx4
#15
Isabel Soria-Bretones, Cristina Cepeda-García, Cintia Checa-Rodriguez, Vincent Heyer, Bernardo Reina-San-Martin, Evi Soutoglou, Pablo Huertas
DNA breaks are complex DNA lesions that can be repaired by two alternative mechanisms: non-homologous end-joining and homologous recombination. The decision between them depends on the activation of the DNA resection machinery, which blocks non-homologous end-joining and stimulates recombination. On the other hand, post-translational modifications play a critical role in DNA repair. We have found that the SUMO E3 ligase CBX4 controls resection through the key factor CtIP. Indeed, CBX4 depletion impairs CtIP constitutive sumoylation and DNA end processing...
July 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28734548/ubiquitin-like-modifications-in-the-dna-damage-response
#16
REVIEW
Zhifeng Wang, Wei-Guo Zhu, Xingzhi Xu
Genomic DNA is damaged at an extremely high frequency by both endogenous and environmental factors. An improper response to DNA damage can lead to genome instability, accelerate the aging process and ultimately cause various human diseases, including cancers and neurodegenerative disorders. The mechanisms that underlie the cellular DNA damage response (DDR) are complex and are regulated at many levels, including at the level of post-translational modification (PTM). Since the discovery of ubiquitin in 1975 and ubiquitylation as a form of PTM in the early 1980s, a number of ubiquitin-like modifiers (UBLs) have been identified, including small ubiquitin-like modifiers (SUMOs), neural precursor cell expressed, developmentally down-regulated 8 (NEDD8), interferon-stimulated gene 15 (ISG15), human leukocyte antigen (HLA)-F adjacent transcript 10 (FAT10), ubiquitin-fold modifier 1 (UFRM1), URM1 ubiquitin-related modifier-1 (URM1), autophagy-related protein 12 (ATG12), autophagy-related protein 8 (ATG8), fan ubiquitin-like protein 1 (FUB1) and histone mono-ubiquitylation 1 (HUB1)...
July 11, 2017: Mutation Research
https://www.readbyqxmd.com/read/28726589/proteomic-study-of-sumoylation-during-solanum-tuberosum-phytophthora-infestans-in-compatible-interactions
#17
Bertrand Colignon, Marc Dieu, Catherine Demazy, Edouard Delaive, Yordan Muhovski, Martine Raes, Sergio Mauro
Invasive plant pathogens have developed the ability to modify the metabolism of their host, promoting metabolic processes which facilitate the growth of the pathogen; at the general expense of the host. The particular enzymatic process SUMOylation which performs post-translational modification of target proteins, leading to changes in many aspects of protein activity and hence metabolism has been demonstrated to be active in many eukaryotic organisms, both animals and plants. Here we provide experimental evidence which indicates that in leaves of Solanum tuberosum which have been infected by Phytophthora infestans, the SUMO pathway enzymes of the host are partially under transcriptional control exerted by the oomycete...
July 20, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28713906/chronic-administration-of-sumo%C3%A2-1-has-negative-effects-on-novel-object-recognition-memory-as-well-as-cell-proliferation-and-neuroblast-differentiation-in-the-mouse-dentate-gyrus
#18
Dae Young Yoo, Dae Won Kim, Hyun Jung Kwon, Hyo Young Jung, Sung Min Nam, Jong Whi Kim, Jin Young Chung, Moo-Ho Won, Yeo Sung Yoon, Soo Young Choi, In Koo Hwang
Post‑translational modifications have been associated with developmental and aging processes, as well as in the pathogenesis of certain diseases. The present study aimed to investigate the effects of small ubiquitin‑like modifier 1 (SUMO‑1) on hippocampal dependent memory function, cell proliferation and neuroblast differentiation. To facilitate the delivery of SUMO‑1 into hippocampal neurons, a transactivator of transcription (Tat)‑SUMO‑1 fusion protein was constructed and mice were divided into two groups: A vehicle (Tat peptide)‑treated group and a Tat‑SUMO‑1‑treated group...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713891/hypothermia-exerts-early-neuroprotective-effects-involving-protein-conjugation-of-sumo%C3%A2-2-3-in-a-rat-model-of-middle-cerebral-artery-occlusion
#19
Gang Li, Xiaozhi Liu, Zhiguo Su, Dong Zhang
How hypothermia serves an early protective role against cerebral ischemia remains to be determined. The small ubiquitin‑related modifier protein (SUMO) functions as a post‑translational modification system and SUMO‑2/3 subtypes are often activated in early stress. The present study investigated changes in the protein level of SUMO using western blotting and immunocytochemistry when neurons were exposed to oxygen‑glucose deprivation (OGD) in vitro, as well as in a rat model of middle cerebral artery occlusion (MCAO) in vivo...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28712389/rescuing-p53-from-mdm2-by-intrinsically-unfolded-sumo-protease-4
#20
Do-Hyoung Kim, Chewook Lee, Bom Kim, Si-Hyung Lee, Kyou-Hoon Han
Many intrinsically unstructured/unfolded proteins (IUPs) contain transient local secondary structures even though they are "unstructured" in a tertiary sense. These local secondary structures are named "pre-structured motifs (PreSMos)" and in fact are the specificity determinants for IUP-target binding, i.e., the active sites in IUPs. Using high-resolution NMR we have delineated a PreSMo active site in the intrinsically unfolded mid-domain (residues 201-300) of SUMO-specific protease 4 (SUSP4). This 29-residue motif which we termed a p53 rescue motif can protect p53 from mdm2 quenching by binding to the p53-helix binding pocket in mdm2(3-109)...
July 17, 2017: BMB Reports
keyword
keyword
22336
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"