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https://www.readbyqxmd.com/read/28436264/interventions-to-improve-grandparent-caregivers-mental-and-physical-health-an-integrative-review
#1
Jen'nea Sumo, JoEllen Wilbur, Wrenetha Julion, Susan Buchholz, Michael Schoeny
The aim of this integrative review is to appraise grandparent caregiver interventions that are designed to improve their physical and mental health. A database search was performed to identify relevant studies published between January 1, 1980, and December 31, 2014. Thirteen publications, including 11 studies, met all inclusion and exclusion criteria. All studies included grandparent mental health outcomes with fewer focusing on physical health and social relations. Improvements were found in all three areas with fewer improvements seen in physical health...
April 1, 2017: Western Journal of Nursing Research
https://www.readbyqxmd.com/read/28435066/cross-talk-between-sumoylation-and-phosphorylation-in-mouse-spermatocytes
#2
Yuxuan Xiao, Benjamin Lucas, Elana Molcho, Margarita Vigodner
The meiotic G2/M1 transition is mostly regulated by posttranslational modifications, however, the cross-talk between different posttranslational modifications is not well-understood, especially in spermatocytes. Sumoylation has emerged as a critical regulatory event in several developmental processes, including reproduction. In mouse oocytes, inhibition of sumoylation caused various meiotic defects and led to aneuploidy. However, the role of sumoylation in male reproduction has only begun to be elucidated. Given the important role of several SUMO targets (including kinases) in meiosis, in this study, the role of sumoylation was addressed by monitoring the G2/M1 transition in pachytene spermatocytes in vitro upon inhibition of sumoylation...
April 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28433946/the-activities-of-the-e3-ubiquitin-ligase-cop1-spa-a-key-repressor-in-light-signaling
#3
REVIEW
Ute Hoecker
Light is a critical signal to integrate plant growth and development with the environment. Downstream of photoreceptors, the E3 ubiquitin ligase COP1/SPA is a key repressor of photomorphogenesis which targets many positive regulators of light signaling, mainly transcription factors, for degradation in darkness. In light-grown plants COP1/SPA activity is repressed, allowing light responses to occur. This review provides an overview on our current knowledge on COP1/SPA repressor function, focusing in particular on the roles of the respective protein domains and the mechanisms of light-induced inactivation of COP1/SPA...
April 20, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28432497/immobilization-of-ulp1-protease-on-nhs-activated-sepharose-a-useful-tool-for-cleavage-of-the-sumo-tag-of-recombinant-proteins
#4
Qiujin Liang, Zhengzhi Huang, Yuan Zhang, Hongtao Li
OBJECTIVE: To fabricate an active and stable enzyme through covalent immobilization, a Ubl-specific protease (Ulp1) was used to cleave small ubiquitin-like modifier (SUMO) fusion proteins. RESULTS: We immobilized Ulp1 on N-hydroxysuccinimide (NHS)-activated Sepharose with a coupling efficiency of 1.7 mg/ml. The immobilized Ulp1 maintains 95% substrate-cleavage ability and significantly enhances pH and thermal stability, especially can withstand pH of 10.5. Besides resistance against some small molecules, the immobilized Ulp1 can tolerate 15% (v/v) DMSO and 20% (v/v) ethanol...
April 21, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28429743/novel-sumo-protease-senp7s-regulates-%C3%AE-catenin-signaling-and-mammary-epithelial-cell-transformation
#5
Samaneh Karami, Feng-Ming Lin, Santosh Kumar, Shaymaa Bahnassy, Hariprasad Thangavel, Maram Quttina, Yue Li, Jing Ren, Tasneem Bawa-Khalfe
SUMO post-translational modification of proteins or SUMOylation ensures normal cell function. Disruption of SUMO dynamics prompts various pathophysiological conditions, including cancer. The burden of deSUMOylating the large SUMO-proteome rests on 6 full-length mammalian SUMO-proteases or SENP. While multiple SENP isoforms exist, the function of these isoforms remains undefined. We now delineate the biological role of a novel SENP7 isoform SENP7S in mammary epithelial cells. SENP7S is the predominant SENP transcript in human mammary epithelia but is significantly reduced in precancerous ductal carcinoma in situ and all breast cancer subtypes...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28423498/the-pias3-smurf2-sumoylation-pathway-suppresses-breast-cancer-organoid-invasiveness
#6
Amrita Singh Chandhoke, Ayan Chanda, Kunal Karve, Lili Deng, Shirin Bonni
Tumor metastasis profoundly reduces the survival of breast cancer patients, but the mechanisms underlying breast cancer invasiveness and metastasis are incompletely understood. Here, we report that the E3 ubiquitin ligase Smurf2 acts in a sumoylation-dependent manner to suppress the invasive behavior of MDA-MB-231 human breast cancer cell-derived organoids. We also find that the SUMO E3 ligase PIAS3 inhibits the invasive growth of breast cancer cell-derived organoids. In mechanistic studies, PIAS3 maintains breast cancer organoids in a non-invasive state via sumoylation of Smurf2...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28419593/protein-sumoylation-and-phosphorylation-intersect-in-arabidopsis-signaling
#7
Ella Nukarinen, Konstantin Tomanov, Ionida Ziba, Wolfram Weckwerth, Andreas Bachmair
Conjugation of the small ubiquitin-related modifier SUMO to protein substrates impacts on stress responses and on development. We analyzed the proteome and phosphoproteome of mutants in this pathway. Mutants chosen had defects in SUMO ligase SIZ1, which catalyzes attachment of single SUMO moieties onto substrates, and in ligases PIAL1 and 2, which are known to form SUMO chains. 2657 proteins and 550 phosphopeptides were identified and quantified. Approximately 40% of the proteins and 20% of the phosphopeptides showed abundance differences in at least one of the analyzed genotypes, demonstrating the influence of SUMO conjugation on protein abundance and phosphorylation...
April 17, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28417919/sumo-specific-cysteine-protease-1-promotes-epithelial-mesenchymal-transition-of-prostate-cancer-cells-via-regulating-smad4-desumoylation
#8
Xiaoyan Zhang, Hao Wang, Hua Wang, Fengjun Xiao, Prem Seth, Weidong Xu, Qinghua Jia, Chutse Wu, Yuefeng Yang, Lisheng Wang
In advanced prostate cancer, small ubiquitin-like modifier (SUMO)-specific cysteine protease 1 (SENP1) is up-regulated. However, the role of SENP1 in regulating deSUMOylation of TGF-β/SMADs signaling is unknown. In this study, we developed a lentiviral vector, PLKO.1-shSENP1, to silence SENP1 in prostate cancer cells with high metastatic characteristics (PC3M). Likewise, we also created an adenovirus vector, Ad5/F11p-SENP1 to over-express SENP1 in prostate cancer cells with low metastatic potential (LNCaP)...
April 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28414785/the-epstein-barr-virus-mir-bhrf1-1-targets-rnf4-during-productive-infection-to-promote-the-accumulation-of-sumo-conjugates-and-the-release-of-infectious-virus
#9
Jinlin Li, Simone Callegari, Maria G Masucci
Post-translational modification by the Small Ubiquitin-like Modifier (SUMO) regulates a variety of cellular functions, and is hijacked by viruses to remodel the host cell during latent and productive infection. Here we have monitored the activity of the SUMO conjugation machinery in cells productively infected with Epstein-Barr virus (EBV). We found that SUMO2/3 conjugates accumulate during the late phase of the productive virus cycle, and identified several viral proteins as bone fide SUMOylation substrates...
April 17, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28412963/posttranslationally-modified-progesterone-receptors-direct-ligand-specific-expression-of-breast-cancer-stem-cell-associated-gene-programs
#10
Todd P Knutson, Thu H Truong, Shihong Ma, Nicholas J Brady, Megan E Sullivan, Ganesh Raj, Kathryn L Schwertfeger, Carol A Lange
BACKGROUND: Estrogen and progesterone are potent breast mitogens. In addition to steroid hormones, multiple signaling pathways input to estrogen receptor (ER) and progesterone receptor (PR) actions via posttranslational events. Protein kinases commonly activated in breast cancers phosphorylate steroid hormone receptors (SRs) and profoundly impact their activities. METHODS: To better understand the role of modified PRs in breast cancer, we measured total and phospho-Ser294 PRs in 209 human breast tumors represented on 2754 individual tissue spots within a tissue microarray and assayed the regulation of this site in human tumor explants cultured ex vivo...
April 17, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28402272/ginkgolic-acids-inhibit-migration-in-breast-cancer-cells-by-inhibition-of-nemo-sumoylation-and-nf-%C3%AE%C2%BAb-activity
#11
Sami Hamdoun, Thomas Efferth
Ginkgolic acids (GA), a group of alkyl phenols found in crude extracts of Ginkgo biloba leaves, are known to have anticancer activity, but their mode of action is not well understood. Our aim in this study was to investigate the anti-migratory activity of seven GA against breast cancer cells and to determine the molecular mechanism behind this activity. All seven GA and their mixture inhibited wound healing in MCF-7 and MDA-MB 231 breast cancer cells. None of the compounds nor the mixture showed cytotoxicity towards the two cell lines, if tested by the resazurin assay...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28400296/recombinant-expression-and-purification-of-the-rna-binding-larp6-proteins-from-fish-genetic-model-organisms
#12
José M Castro, Daniel A Horn, Karen A Lewis
The RNA-binding proteins that comprise the La-related protein (LARP) superfamily have been implicated in a wide range of cellular functions, from tRNA maturation to regulation of protein synthesis. To more expansively characterize the biological function of the LARP6 subfamily, we have recombinantly expressed the full-length LARP6 proteins from two teleost fish, platyfish (Xiphophorus maculatus) and zebrafish (Danio rerio). The yields of the recombinant proteins were enhanced to >2 mg/L using a tandem approach of an N-terminal His6-SUMO tag and an iterative solubility screening assay to identify structurally stabilizing buffer components...
April 8, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28382294/the-correlation-between-preoperative-volumetry-and-real-graft-weight-comparison-of-two-volumetry-programs
#13
Nadiar Mussin, Marco Sumo, Kwang-Woong Lee, YoungRok Choi, Jin Yong Choi, Sung-Woo Ahn, Kyung Chul Yoon, Hyo-Sin Kim, Suk Kyun Hong, Nam-Joon Yi, Kyung-Suk Suh
PURPOSE: Liver volumetry is a vital component in living donor liver transplantation to determine an adequate graft volume that meets the metabolic demands of the recipient and at the same time ensures donor safety. Most institutions use preoperative contrast-enhanced CT image-based software programs to estimate graft volume. The objective of this study was to evaluate the accuracy of 2 liver volumetry programs (Rapidia vs. Dr. Liver) in preoperative right liver graft estimation compared with real graft weight...
April 2017: Annals of Surgical Treatment and Research
https://www.readbyqxmd.com/read/28379520/sumo-conjugation-to-spliceosomal-proteins-is-required-for-efficient-pre-mrna-splicing
#14
Berta Pozzi, Laureano Bragado, Cindy L Will, Pablo Mammi, Guillermo Risso, Henning Urlaub, Reinhard Lührmann, Anabella Srebrow
Pre-mRNA splicing is catalyzed by the spliceosome, a multi-megadalton ribonucleoprotein machine. Previous work from our laboratory revealed the splicing factor SRSF1 as a regulator of the SUMO pathway, leading us to explore a connection between this pathway and the splicing machinery. We show here that addition of a recombinant SUMO-protease decreases the efficiency of pre-mRNA splicing in vitro. By mass spectrometry analysis of anti-SUMO immunoprecipitated proteins obtained from purified splicing complexes formed along the splicing reaction, we identified spliceosome-associated SUMO substrates...
March 30, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28379052/regulation-of-transcription-factors-by-sumoylation
#15
Emanuel Rosonina, Akhi Akhter, Yimo Dou, John Babu, Veroni S Sri Theivakadadcham
Transcription factors (TFs) are among the most frequently detected targets of sumoylation, and effects of the modification have been studied for about 200 individual TFs to date. TF sumoylation is most often associated with reduced target gene expression, which can be mediated by enhanced interactions with corepressors or by interference with protein modifications that promote transcription. However, recent studies show that sumoylation also regulates gene expression by controlling the levels of TFs that are associated with chromatin...
April 5, 2017: Transcription
https://www.readbyqxmd.com/read/28373296/a-proteomic-approach-to-identify-alterations-in-the-sumo-network-during-controlled-mechanical-ventilation-in-rat-diaphragm-muscle
#16
Arvind Venkat Namuduri, Gabriel Heras, Jia Mi, Nicola Cacciani, Katarina Hörnaeus, Anne Konzer, Sara Bergström Lind, Lars Larsson, Stefano Gastaldello
The Small Ubiquitin-like Modifier (SUMO) is as a regulator of many cellular functions by reversible conjugation to a broad number of substrates. Under endogenous or exogenous perturbations, the SUMO network becomes a fine sensor of stress conditions by alterations in the expression level of SUMO enzymes and consequently changing the status of SUMOylated proteins. The diaphragm is the major inspiratory muscle, which is continuously active under physiological conditions, but its structure and function is severely affected when passively displaced for long extents during mechanical ventilation (MV)...
April 3, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28358373/myogenic-differentiation-triggers-pml-nuclear-body-loss-and-daxx-relocalization-to-chromocentres
#17
Jayme Salsman, Lindsy M Rapkin, Nandini N Margam, Roy Duncan, David P Bazett-Jones, Graham Dellaire
The promyelocytic leukemia protein (PML) is expressed in most normal human tissues and forms nuclear bodies (NBs) that have roles in gene regulation and cellular processes such as DNA repair, cell cycle control, and cell fate decisions. Using murine C2C12 myoblasts, we demonstrate that activation of skeletal muscle differentiation results in loss of PML and PML NBs prior to myotube fusion. Myotube formation was associated with marked chromatin reorganization and the relocalization of DAXX from PML NBs to chromocentres...
March 30, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28351334/upregulation-of-senp3-smt3ip1-promotes-epithelial-ovarian-cancer-progression-and-forecasts-poor-prognosis
#18
Jialin Cheng, Min Su, Yunfeng Jin, Qinghua Xi, Yan Deng, Jie Chen, Wei Wang, Yannan Chen, Lingling Chen, Nannan Shi, Guoxin Mao
As a crucial member of the small ubiquitin-like modifier system, SUMO-specific protease 3, was identified to be essential for cell proliferation and ribosomal RNA processing. Recent studies showed that SUMO-specific protease 3 was elevated in ovarian cancer compared to normal tissue samples. However, the connection between SUMO-specific protease 3-specific expression and clinicopathological parameters of epithelial ovarian cancer, as well as the physiologically potential role of SUMO-specific protease 3 in epithelial ovarian cancer remained unclear...
March 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28349302/a-mutant-sumo-facilitates-quick-plasmid-construction-for-expressing-proteins-with-native-n-termini-after-tag-removal
#19
Yuzhu Zhang, Yuting Fan
Sumo is one of the fusion tags commonly used to enhance the expression and the solubility of recombinant proteins. One advantage of using sumo is that the removal of the sumo tag is highly specific because its recognition by a sumo protease is determined by its structural characteristics, instead of the sequence of a short peptide. Recently, it was reported that sumo could also be used as a protease recognition site to facilitate the removal of other fusion tags, such as MBP, when sumo itself is not suitable to enhance the solubility of a particular target protein...
March 27, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28346086/a-small-molecule-microarray-approach-for-the-identification-of-e2-enzyme-inhibitors-in-ubiquitin-like-conjugation-pathways
#20
Katherine Zlotkowski, William M Hewitt, Ranu S Sinniah, Joseph E Tropea, Danielle Needle, George T Lountos, Joseph J Barchi, David S Waugh, John S Schneekloth
E2 enzymes in ubiquitin-like conjugation pathways are important, highly challenging pharmacological targets, and despite significant efforts, few noncovalent modulators have been discovered. Small-molecule microarray (SMM)-based screening was employed to identify an inhibitor of the "undruggable" small ubiquitin-like modifier (SUMO) E2 enzyme Ubc9. The inhibitor, a degradation product from a commercial screening collection, was chemically synthesized and evaluated in biochemical, mechanistic, and structure-activity relationship studies...
January 1, 2017: SLAS Discovery
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