Jielin Chen, Zheze Dai, Hui Lv, Zhongchao Jin, Yuqing Tang, Xiaodong Xie, Jiye Shi, Fei Wang, Qian Li, Xiaoguo Liu, Chunhai Fan
Self-assembled DNA crystals offer a precise chemical platform at the ångström-scale for DNA nanotechnology, holding enormous potential in material separation, catalysis, and DNA data storage. However, accurately controlling the crystallization kinetics of such DNA crystals remains challenging. Herein, we found that atomic-level 5-methylcytosine (5mC) modification can regulate the crystallization kinetics of DNA crystal by tuning the hybridization rates of DNA motifs. We discovered that by manipulating the axial and combination of 5mC modification on the sticky ends of DNA tensegrity triangle motifs, we can obtain a series of DNA crystals with controllable morphological features...
March 12, 2024: Proceedings of the National Academy of Sciences of the United States of America