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plasma DNase1

Larissa Berloffa Belardin, Paula Toni Del Giudice, Mariana Camargo, Paula Intasqui, Mariana Pereira Antoniassi, Ricardo Pimenta Bertolla, Agnaldo Pereira Cedenho
PURPOSE: To verify if the presence of varicocele (grades II and III) with and without seminal alterations, using the 5(th) centile cutoff values in table A1.1 of the World Health Organization (WHO, 2010) manual, alters the seminal plasma levels of proteins DNASE1 (deoxyribonuclease-1) and IGFBP7 (Insulin-like growth factor-binding protein 7), which are related to apoptosis regulation and cell proliferation, respectively, demonstrating that these proteins are important for correct spermatogenesis...
September 14, 2016: Journal of Assisted Reproduction and Genetics
Inna B Korzeneva, Svetlana V Kostuyk, Liza S Ershova, Andrian N Osipov, Veronika F Zhuravleva, Galina V Pankratova, Lev N Porokhovnik, Natalia N Veiko
The blood plasma of healthy people contains cell-fee (circulating) DNA (cfDNA). Apoptotic cells are the main source of the cfDNA. The cfDNA concentration increases in case of the organism's cell death rate increase, for example in case of exposure to high-dose ionizing radiation (IR). The objects of the present research are the blood plasma and blood lymphocytes of people, who contacted occupationally with the sources of external gamma/neutron radiation or internal β-radiation of tritium N = 176). As the controls (references), blood samples of people, who had never been occupationally subjected to the IR sources, were used (N = 109)...
September 2015: Mutation Research
M Jiménez-Alcázar, M Napirei, R Panda, E C Köhler, J A Kremer Hovinga, H G Mannherz, S Peine, T Renné, B Lämmle, T A Fuchs
BACKGROUND: Acute thrombotic microangiopathies (TMAs) are characterized by excessive microvascular thrombosis and are associated with markers of neutrophil extracellular traps (NETs) in plasma. NETs are composed of DNA fibers and promote thrombus formation through the activation of platelets and clotting factors. OBJECTIVE: The efficient removal of NETs may be required to prevent excessive thrombosis such as in TMAs. To test this hypothesis, we investigated whether TMAs are associated with a defect in the degradation of NETs...
May 2015: Journal of Thrombosis and Haemostasis: JTH
Jan Rossaint, Jan M Herter, Hugo Van Aken, Markus Napirei, Yvonne Döring, Christian Weber, Oliver Soehnlein, Alexander Zarbock
There is emerging evidence that neutrophil extracellular traps (NETs) play important roles in inflammatory processes. Here we report that neutrophils have to be simultaneously activated by integrin-mediated outside-in- and G-protein-coupled receptor (GPCR) signaling to induce NET formation in acute lung injury (ALI), which is associated with a high mortality rate in critically ill patients. NETs consist of decondensed chromatin decorated with granular and cytosolic proteins and they can trap extracellular pathogens...
April 17, 2014: Blood
Martin Sillesen, Guang Jin, Rahmi Oklu, Hassan Albadawi, Ayesha M Imam, Cecilie H Jepsen, John O Hwabejire, Sisse R Ostrowski, Pär I Johansson, Lars S Rasmussen, Hasan B Alam
INTRODUCTION: Traumatic brain injury and shock are among the leading causes of trauma-related mortality. We have previously shown that fresh-frozen plasma (FFP) resuscitation reduces the size of brain lesion and associated swelling compared with crystalloids. We hypothesized that this effect would be associated with an attenuation of circulating nucleosome levels, a biomarker of injury with cytotoxic potential, through reconstitution of circulating deoxyribonuclease-1 (DNAse1), an enzyme identified as critical in nucleosome clearance from the circulation...
August 2013: Surgery
Axelle Caudrillier, Kai Kessenbrock, Brian M Gilliss, John X Nguyen, Marisa B Marques, Marc Monestier, Pearl Toy, Zena Werb, Mark R Looney
There is emerging evidence that platelets are major contributors to inflammatory processes through intimate associations with innate immune cells. Here, we report that activated platelets induce the formation of neutrophil extracellular traps (NETs) in transfusion-related acute lung injury (TRALI), which is the leading cause of death after transfusion therapy. NETs are composed of decondensed chromatin decorated with granular proteins that function to trap extracellular pathogens; their formation requires the activation of neutrophils and release of their DNA in a process that may or may not result in neutrophil death...
July 2012: Journal of Clinical Investigation
Yuhua Ni, Jianjun Zhang, Baogui Sun
DNASE1, the encoding gene of deoxyribonuclease I (DNase I), exhibits polymorphisms, including a single nucleotide polymorphism (SNP A2317G) in exon 8 and a 56 bp variable number of tandem repeat, designated as HumDN1 in intron 4. Several different ethnic population studies have revealed both A2317G and HumDN1 demonstrate genetic heterogeneity in the worldwide distribution. Recently, G2317 allele was proposed as an independent risk factor for myocardial infarction in Japanese population. In the present study, we identified A2317G and HumDN1 genotypes in 402 unrelated healthy Han Chinese individuals...
September 2008: Molecular Biology Reports
Dirk Eulitz, Hans Georg Mannherz
Deoxyribonuclease I (Dnase1) is the major extracellular endonuclease. It is secreted by digestive glands into the alimentary tract and into the plasma, lacrimal fluid and urine by hepatocytes, lacrimal glands and renal proximal tubular cells, respectively. In many species the activity of Dnase1 is inhibited by monomeric actin. However, the biological significance of this high affinity interaction is unknown. We generated a Dnase1 mutant with extremely reduced actin binding capacity. EGFP-constructs of wild-type and mutant Dnase1 were transfected into MCF-7 breast cancer cells and apoptosis or necrosis was induced by staurosporine or oxidative stress...
August 2007: Apoptosis: An International Journal on Programmed Cell Death
K Miyake, P L McNeil, K Suzuki, R Tsunoda, N Sugai
Plasma membrane disruption is a common form of cell injury in many normal biological environments, including many mammalian tissues. Survival depends on the initiation of a rapid resealing response that is mounted only in the presence of physiological levels of extracellular Ca(2+). Vesicle-vesicle and vesicle-plasma membrane fusion events occurring in cortical cytoplasm surrounding the defect are thought to be a crucial element of the resealing mechanism. However, in mammalian cells, the vesicles used in this fusion reaction (endosomes/lysosomes) are not present in a 'pre-docked' configuration and so must be brought into physical contact with one another and with the plasma membrane...
October 2001: Journal of Cell Science
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