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https://www.readbyqxmd.com/read/29142517/is-methylation-analysis-of-sfrp2-tfpi2-ndrg4-and-bmp3-promoters-suitable-for-colorectal-cancer-screening-in-the-korean-population
#1
Soo-Kyung Park, Hae Lim Baek, Junghee Yu, Ji Yeon Kim, Hyo-Joon Yang, Yoon Suk Jung, Kyu Yong Choi, Hungdai Kim, Hyung Ook Kim, Kyung Uk Jeong, Ho-Kyung Chun, Kyungeun Kim, Dong Il Park
Background/Aims: Colorectal cancer (CRC) screening using stool DNA was recently found to yield good detection rates. A multi-target stool DNA test (Cologuard®, Exact Sciences), including methylated genes has been recently approved by the U.S. Food and Drug Administration. The aim of this study was to validate these aberrantly methylated genes as stool-based DNA markers for detecting CRC and colorectal advanced adenoma (AA) in the Korean population. Methods: A single-center study was conducted in 36 patients with AA; 35 patients with CRC; and 40 endoscopically diagnosed healthy controls using CRC screening colonoscopy...
October 2017: Intestinal Research
https://www.readbyqxmd.com/read/29131365/gut-microbiota-and-body-composition-in-anorexia-nervosa-inpatients-in-comparison-to-athletes-overweight-obese-and-normal-weight-controls
#2
Sabrina Mörkl, Sonja Lackner, Wolfram Müller, Gregor Gorkiewicz, Karl Kashofer, Andreas Oberascher, Annamaria Painold, Anna Holl, Peter Holzer, Andreas Meinitzer, Harald Mangge, Sandra Holasek
OBJECTIVES: Anorexia nervosa (AN) is a heterogeneous eating disorder associated with alterations of body structure and the gut microbiome. We aimed to investigate the gut microbiota composition of a large female cohort including different BMI groups and activity levels along with body composition parameters. METHOD: 106 female participants were included in this cross-sectional study: AN patients (n = 18), athletes (n = 20), normal weight (n = 26), overweight (n = 22), and obese women (n = 20)...
November 13, 2017: International Journal of Eating Disorders
https://www.readbyqxmd.com/read/29126996/blastocystis-genetic-diversity-among-children-of-low-income-daycare-center-in-southeastern-brazil
#3
Ana Paula Oliveira-Arbex, Érica Boarato David, Semíramis Guimarães
Blastocystis, an unicellular anaerobic eukaryote, is known to be a very common intestinal parasite found in humans and animals fecal samples worldwide. Currently, there is an increasing interest to yield insights into its prevalence and diversity in human populations living in poor and deprived areas. In this study, we describe the prevalence and genetic variability of Blastocystis isolates obtained from daycare center attendees aged 0 to 6years and staff, as well as some children family members and their dogs in a low-income community in São Paulo State, Brazil...
November 7, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29122790/cohort-profile-design-and-first-results-of-the-dutch-ibd-biobank-a-prospective-nationwide-biobank-of-patients-with-inflammatory-bowel-disease
#4
Lieke M Spekhorst, Floris Imhann, Eleonora Am Festen, Ad A van Bodegraven, Nanne Kh de Boer, Gerd Bouma, Herma H Fidder, Geert D'Haens, Frank Hoentjen, Daan W Hommes, Dirk J de Jong, Mark Löwenberg, Pw Jeroen Maljaars, Andrea E van der Meulen-de Jong, Bas Oldenburg, Marieke J Pierik, Cyriel Y Ponsioen, Pieter C Stokkers, Hein W Verspaget, Marijn C Visschedijk, C Janneke van der Woude, Gerard Dijkstra, Rinse K Weersma
PURPOSE: The Dutch IBD Biobank aims to facilitate the discovery of predictors for individual disease course and treatment response in patients with inflammatory bowel disease (IBD). In this paper, we aim to describe the establishment of the Dutch IBD Biobank, including the facilitators and barriers to establishment. Moreover, we aim to provide a complete overview of the content of the Dutch IBD Biobank. PARTICIPANTS: Since 2007, every patient with IBD treated in one of the eight Dutch university medical centres is asked to participate in the Dutch IBD Biobank in which 225 standardised IBD-related data items and biomaterials, such as serum, DNA, biopsies and a stool sample, are collected...
November 8, 2017: BMJ Open
https://www.readbyqxmd.com/read/29120399/a-microbiomic-analysis-in-african-americans-with-colonic-lesions-reveals-streptococcus-sp-vt162-as-a-marker-of-neoplastic-transformation
#5
Hassan Brim, Shibu Yooseph, Edward Lee, Zaki Sherif, Muneer Abbas, Adeyinka O Laiyemo, Sudhir Varma, Manolito Torralba, Scot E Dowd, Karen E Nelson, Wimal Pathmasiri, Susan Sumner, Willem de Vos, Qiaoyi Liang, Jun Yu, Erwin Zoetendal, Hassan Ashktorab
Increasing evidence suggests a role of the gut microbiota in colorectal carcinogenesis (CRC). To detect bacterial markers of colorectal cancer in African Americans a metabolomic analysis was performed on fecal water extracts. DNA from stool samples of adenoma and healthy subjects and from colon cancer and matched normal tissues was analyzed to determine the microbiota composition (using 16S rDNA) and genomic content (metagenomics). Metagenomic functions with discriminative power between healthy and neoplastic specimens were established...
November 9, 2017: Genes
https://www.readbyqxmd.com/read/29118177/detection-of-shigella-in-direct-stool-specimens-using-a-metagenomic-approach
#6
Jie Liu, Mathieu Almeida, Furqan Kabir, Sadia Shakoor, Shahida Qureshi, Anita Zaidi, Shan Li, Boubou Tamboura, Samba O Sow, Inacio Mandomando, Pedro L Alonso, Thandavarayan Ramamurthy, Dipika Sur, Karen Kotloff, James Nataro, Myron M Levine, O Colin Stine, Eric Houpt
The underestimation of Shigella species as a cause of childhood diarrhea disease has become increasingly apparent with quantitative (q)PCR based diagnostic methods versus culture. We sought to confirm qPCR-based detection of Shigella via a metagenomics approach. Three groups of samples were selected from diarrheal cases from the Global Enteric Multicenter Study: 9 Shigella culture positive and qPCR positive (culture+/qPCR+), 9 culture negative but qPCR positive (culture-/qPCR+), 9 culture negative and qPCR negative (culture-/qPCR-)...
November 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29113617/molecular-detection-of-cyclospora-cayetanensis-in-human-stool-specimens-using-unex-based-dna-extraction-and-real-time-pcr
#7
Yvonne Qvarnstrom, Theresa Benedict, Paula L Marcet, Ryan E Wiegand, Barbara L Herwaldt, Alexandre J da Silva
Cyclospora cayetanensis is a coccidian parasite associated with diarrheal illness. In the USA, foodborne outbreaks of cyclosporiasis have been documented almost every year since the mid-1990s. The typical approach used to identify this parasite in human stools is an examination of acid-fast-stained smears under bright-field microscopy. UV fluorescence microscopy of wet mounts is more sensitive and specific than acid-fast staining but requires a fluorescence microscope with a special filter not commonly available in diagnostic laboratories...
November 8, 2017: Parasitology
https://www.readbyqxmd.com/read/29093617/detection-of-kras-g12d-in-colorectal-cancer-stool-by-droplet-digital-pcr
#8
Susana Olmedillas-López, Dennis César Lévano-Linares, Carmen Laura Aúz Alexandre, Luz Vega-Clemente, Edurne León Sánchez, Alejandro Villagrasa, Jaime Ruíz-Tovar, Mariano García-Arranz, Damián García-Olmo
AIM: To assess KRAS G12D mutation detection by droplet digital PCR (ddPCR) in stool-derived DNA from colorectal cancer (CRC) patients. METHODS: In this study, tumor tissue and stool samples were collected from 70 patients with stage I-IV CRC diagnosed by preoperative biopsy. KRAS mutational status was determined by pyrosequencing analysis of DNA obtained from formalin-fixed paraffin-embedded (FFPE) tumor tissues. The KRAS G12D mutation was then analyzed by ddPCR in FFPE tumors and stool-derived DNA from patients with this point mutation...
October 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29070463/-structural-changes-of-gut-microbiota-after-gastric-bypass-in-obese-patients-with-type-2-diabetes
#9
Min Lang, Ke-Hong Zheng, Song-Hua Zeng, Yuan Yang, Liang-Ping Wu, Zong-Hai Huang
OBJECTIVE: To investigate the structural changes inintestinal flora and metabolic changes in type 2 diabetic patients with obesity(BMI≥40 kg/m(2))by sequencing the 16S rRNA genes. METHODS: Stool samples were collected from 4 diabetic patients before and after gastric bypass surgery for extraction of the total DNA. The diversity of the intestinal flora in the samples was investigated by 16S rRNA sequencing. After surgery, the changes in glucose and lipid metabolism were evaluated in the patients, and the changesin body mass index (BMI) and waist to hip ratio were assessed at 3 month intervals...
October 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29070053/distribution-of-blastocystis-subtypes-isolated-from-humans-from-an-urban-community-in-rio-de-janeiro-brazil
#10
Carolina Valença Barbosa, Rosemary de Jesus Batista, Ricardo Pereira Igreja, Claudia Masini d'Avila Levy, Heloisa Werneck de Macedo, Helena Lúcia Carneiro Santos
BACKGROUND: Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil...
October 25, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29067276/characteristics-of-clostridium-difficile-infection-in-patients-hospitalized-with-myelodysplastic-syndrome-or-acute-myelogenous-leukemia
#11
Kamini Shah, Bryan F Curtin, Christopher Chu, Daniel Hwang, Mark H Flasar, Erik von Rosenvinge
AIM: To evaluate factors associated with Clostridium difficile infection (CDI) and outcomes of CDI in the myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) population. METHODS: After IRB approval, all MDS/AML patients hospitalized at the University of Maryland Greenebaum Comprehensive Cancer Center between August 2011 and December 2013 were identified. Medical charts were reviewed for demographics, clinical information, development of CDI, complications of CDI, and mortality...
October 10, 2017: World Journal of Clinical Oncology
https://www.readbyqxmd.com/read/29049378/immune-modulatory-genomic-properties-differentiate-gut-microbiota-of-infants-with-and-without-eczema
#12
Seungdae Oh, Gaik Chin Yap, Pei-Ying Hong, Chiung-Hui Huang, Marion M Aw, Lynette Pei-Chi Shek, Wen-Tso Liu, Bee Wah Lee
Gut microbiota play an important role in human immunological processes, potentially affecting allergic diseases such as eczema. The diversity and structure of gut microbiota in infants with eczema have been previously documented. This study aims to evaluate by comparative metagenomics differences in genetic content in gut microbiota of infants with eczema and their matched controls. Stools were collected at the age of one month old from twelve infants from an at risk birth cohort in a case control manner. Clinical follow up for atopic outcomes were carried out at the age of 12 and 24 months...
2017: PloS One
https://www.readbyqxmd.com/read/29047387/isothermal-diagnostic-assays-for-the-detection-of-soil-transmitted-helminths-based-on-the-smartamp2-method
#13
Nour Rashwan, Aïssatou Diawara, Marilyn E Scott, Roger K Prichard
BACKGROUND: Diagnosis of soil-transmitted helminths (STHs) has traditionally relied on stool microscopy, which has a number of critical deficiencies. Molecular diagnostics are powerful tools to identify closely related species, but the requirement for costly equipment makes their implementation difficult in low-resource or field settings. Rapid, sensitive and cost-effective diagnostic tools are crucial for accurate estimation of STH infection intensity in MDA programmes in which the goal is to reduce morbidity following repeated rounds of chemotherapy...
October 18, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29039846/gut-bacterial-communities-of-diarrheic-patients-with-indications-of-clostridioides-difficile-infection
#14
Dominik Schneider, Andrea Thürmer, Kathleen Gollnow, Raimond Lugert, Katrin Gunka, Uwe Groß, Rolf Daniel
We present bacterial 16S rRNA gene datasets derived from stool samples of 44 patients with diarrhea indicative of a Clostridioides difficile infection. For 20 of these patients, C. difficile infection was confirmed by clinical evidence. Stool samples from patients originating from Germany, Ghana, and Indonesia were taken and subjected to DNA isolation. DNA isolations of stool samples from 35 asymptomatic control individuals were performed. The bacterial community structure was assessed by 16S rRNA gene analysis (V3-V4 region)...
October 17, 2017: Scientific Data
https://www.readbyqxmd.com/read/29035858/detection-of-small-number-of-giardia-in-biological-materials-prepared-from-stray-dogs
#15
Leila Esmailikia, Elahe Ebrahimzade, Parviz Shayan, Narges Amininia
Giardia lamblia is an intestinal protozoa with intermittent and low shedding especially in dogs, and the detection of Giardia is accompanied with problems such as sampling and diagnostic method. The objective of this study was to detection of Giardia in biological materials with low number of parasite using parasitological and molecular methods, and also to determine whether the examined stray dogs harbor known zoonotic genotype of Giardia. For this aim 85 fecal and duodenal samples were studied from which 1 was positive by Trichrome staining of stool, 4 were positive by staining of duodenal samples...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29029307/droplet-digital-pcr-diagnosis-of-human-schistosomiasis-parasite-cell-free-dna-detection-in-diverse-clinical-samples
#16
Kosala G Weerakoon, Catherine A Gordon, Gail M Williams, Pengfei Cai, Geoffrey N Gobert, Remigio M Olveda, Allen G Ross, David U Olveda, Donald P McManus
Background: Schistosomiasis japonica remains a major public health and socio-economic concern in Southeast-Asia. Sensitive and accurate diagnostics can play a pivotal role in achieving disease elimination goals. Methods: We previously reported a novel droplet digital-PCR (ddPCR) assay targeting the mitochondrial gene nad1 to diagnose schistosomiasis japonica. The tool identified both pre-patent and patent infections using Schistosoma japonicum DNA isolated from serum, urine, salivary glands and faeces in a murine model...
September 27, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29025731/macrophage-depletion-using-clodronate-liposomes-decreases-tumorigenesis-and-alters-gut-microbiota-in-the-aom-dss-mouse-model-of-colon-cancer
#17
Jackie E Bader, Reilly T Enos, Kandy T Velazquez, Meredith S Carson, Mitzi Nagarkatti, Prakash S Nagarkatti, Ioulia Chatzistamou, J Mark Davis, James A Carson, Cory Robinson, E Angela Murphy
We examined the role of macrophages in inflammation associated colorectal cancer (CRC). Given the emerging evidence on immune-microbiota interactions in CRC, we also sought to examine the interaction between macrophages and gut microbiota. To induce CRC, male C57BL/6 mice (n=32) received a single injection of azoxymethane (AOM) followed by three cycles of dextran sodium sulfate (DSS) supplemented water at weeks 1, 4, and 7, respectively. Prior to the final DSS cycle (week 7) and twice weekly until sacrifice, mice (n=16/group) received either 200μl i...
October 12, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/29021787/integron-digestive-carriage-in-human-and-cattle-a-one-health-cultivation-independent-approach
#18
Delphine Chainier, Olivier Barraud, Geoffrey Masson, Elodie Couve-Deacon, Bruno François, Claude-Yves Couquet, Marie-Cécile Ploy
Objectives: Dissemination of antimicrobial resistance (AMR) is a global issue that requires the adoption of a "One-Health" approach promoting integration of human and animal health. Besides culture-dependent techniques frequently used for AMR surveillance, cultivation-independent methods can give additional insights into the diversity and reservoir of AMR genetic determinants. Integrons are molecular markers that can provide overall and reliable estimation of AMR dissemination. In this study, considering the "One-Health" approach, we have analyzed the integron digestive carriage from stools of humans and cattle living in a same area and exposed to different antibiotic selection pressures...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29016326/human-intestinal-parasite-burden-and-poor-sanitation-in-rural-alabama
#19
Megan L McKenna, Shannon McAtee, Patricia E Bryan, Rebecca Jeun, Tabitha Ward, Jacob Kraus, Maria E Bottazzi, Peter J Hotez, Catherine C Flowers, Rojelio Mejia
Hookworm infection affects 430 million people worldwide, causing iron deficiency, impaired cognitive development, and stunting in children. Because of the environmental conditions needed for the hookworm life-cycle, this parasite is endemic to resource-limited countries. Necator americanus was endemic in the southern United States before improvement of sewage disposal systems and eradication programs. With continued poverty, poor sanitation, and an environment suitable for the hookworm life-cycle in some regions of the southern United States, a current prevalence study using modern molecular diagnostics is warranted...
September 5, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28991924/application-of-a-loop-mediated-isothermal-amplification-lamp-assay-targeting-cox1-gene-for-the-detection-of-clonorchis-sinensis-in-human-fecal-samples
#20
S M Mazidur Rahman, Hyun Beom Song, Yan Jin, Jin-Kyoung Oh, Min Kyung Lim, Sung-Tae Hong, Min-Ho Choi
BACKGROUND: Clonorchiasis is prevalent in the Far East, and a major health problem in endemic areas. Infected persons may experience, if not treated, serious complications such as bile stone formation, pyogenic cholangitis, and even cholangiocarcinoma. Early diagnosis and treatment are important to prevent serious complications and, therefore, the simple and reliable diagnostic method is necessary to control clonorchiasis in endemic areas, where resources for the diagnosis are limited...
October 2017: PLoS Neglected Tropical Diseases
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