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https://www.readbyqxmd.com/read/28723708/subclinical-herpesvirus-shedding-among-hiv-1-infected-men-on-antiretroviral-therapy
#1
Arcadio Agudelo-Hernandez, Yue Chen, Arlene Bullotta, William G Buchanan, Cynthia R Klamar-Blain, Luann Borowski, Sharon A Riddler, Charles R Rinaldo, Bernard Jc Macatangay
DESIGN: We evaluated the subclinical shedding of 6 different herpesviruses in antiretroviral drug-treated HIV(+) men who have sex with men (MSM), and determined how this is associated with markers of inflammation and immune activation. METHODS: We obtained blood, semen, throat washing, urine, and stool from 15 antiretroviral-treated HIV-1-infected MSM with CD4 T cell reconstitution, and 12 age-matched HIV(-) MSM from the Multicenter AIDS Cohort Study at 4 timepoints over 24 weeks to measure DNA levels of cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus 1 and 2, human herpesvirus 6 (HHV6), and HHV8...
July 18, 2017: AIDS
https://www.readbyqxmd.com/read/28719952/molecular-phylogenetics-of-trichostrongylus-species-nematoda-trichostrongylidae-from-humans-of-mazandaran-province-iran
#2
Meysam Sharifdini, Zahra Heidari, Zahra Hesari, Sajad Vatandoost, Eshrat Beigom Kia
The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences...
June 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28713847/next-generation-sequencing-of-the-dna-virome-from-fecal-samples
#3
Cynthia L Monaco, Douglas S Kwon
Herein we describe a detailed protocol for DNA virome analysis of low input human stool samples (Monaco et al., 2016). This protocol is divided into four main steps: 1) stool samples are pulverized to evenly distribute microbial matter; 2) stool is enriched for virus-like particles and DNA is extracted by phenol-chloroform; 3) purified DNA is multiple-strand displacement amplified (MDA) and fragmented; and 4) libraries are constructed and sequenced using Illumina Miseq. Subsequent sequence analysis for viral sequence identification should be sensitive but stringent...
March 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28707271/identification-and-genetic-characterization-of-a-novel-circular-single-stranded-dna-virus-in-a-human-upper-respiratory-tract-sample
#4
Lunbiao Cui, Binyao Wu, Xiaojuan Zhu, Xiling Guo, Yiyue Ge, Kangchen Zhao, Xian Qi, Zhiyang Shi, Fengcai Zhu, Lixin Sun, Minghao Zhou
Metagenomic analysis through high-throughput sequencing is a tool for detecting both known and novel viruses. Using this technique, a novel circular single-stranded DNA (ssDNA) virus genome was discovered in respiratory secretions from a febrile traveler. The virus, named human respiratory-associated PSCV-5-like virus (HRAPLV), has a genome comprising 3,018 bases, with two major putative ORFs inversely encoding capsid (Cap) and replicase (Rep) protein and separated by two intergenic regions. One stem-loop structure was predicted in the larger intergenic region (LIR)...
July 13, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28700744/hypermethylated-dna-a-circulating-biomarker-for-colorectal-cancer-detection
#5
Simon Ladefoged Rasmussen, Henrik Bygum Krarup, Kåre Gotschalck Sunesen, Martin Berg Johansen, Mogens Tornby Stender, Inge Søkilde Pedersen, Poul Henning Madsen, Ole Thorlacius-Ussing
BACKGROUND: Colorectal cancer (CRC) is one of the most common cancers in the western world. Screening is an efficient method of reducing cancer-related mortality. Molecular biomarkers for cancer in general and CRC in particular have been proposed, and hypermethylated DNA from stool or blood samples are already implemented as biomarkers for CRC screening. We aimed to evaluate the performance of proven hypermethylated DNA promoter regions as plasma based biomarkers for CRC detection. METHODS: We conducted a cross-sectional case-control study of 193 CRC patients and 102 colonoscopy-verified healthy controls...
2017: PloS One
https://www.readbyqxmd.com/read/28700459/the-microbiome-in-posttraumatic-stress-disorder-and-trauma-exposed-controls-an-exploratory-study
#6
Sian M J Hemmings, Stefanie Malan-Muller, Leigh L van den Heuvel, Brittany A Demmitt, Maggie A Stanislawski, David G Smith, Adam D Bohr, Christopher E Stamper, Embriette R Hyde, James T Morton, Clarisse A Marotz, Philip H Siebler, Maarten Braspenning, Wim Van Criekinge, Andrew J Hoisington, Lisa A Brenner, Teodor T Postolache, Matthew B McQueen, Kenneth S Krauter, Rob Knight, Soraya Seedat, Christopher A Lowry
OBJECTIVE: Inadequate immunoregulation and elevated inflammation may be risk factors for posttraumatic stress disorder (PTSD), and microbial inputs are important determinants of immunoregulation; however, the association between the gut microbiota and PTSD is unknown. This study investigated the gut microbiome in a South African sample of PTSD-affected individuals and trauma-exposed (TE) controls, to identify potential differences in microbial diversity or microbial community structure...
July 11, 2017: Psychosomatic Medicine
https://www.readbyqxmd.com/read/28698878/bacterial-community-profile-of-the-gut-microbiota-differs-between-hypercholesterolemic-subjects-and-controls
#7
Camilo Rebolledo, Alejandro Cuevas, Tomás Zambrano, Jacquelinne J Acuña, Milko A Jorquera, Kathleen Saavedra, Claudia Martínez, Fernando Lanas, Pamela Serón, Luis A Salazar, Nicolás Saavedra
The role of gut microbiota in the development of metabolic illnesses has been abundantly demonstrated. Recent studies suggest that gut microbiota alterations may also be related to the development of hypercholesterolemia. Therefore, we aimed to assess differences in the gut bacterial community profiles between hypercholesterolemic subjects and controls. Thirty cases diagnosed with hypercholesterolemia and 27 normocholesterolemic controls were included. A fasting whole blood sample was obtained to determine the lipid profile...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28692668/a-novel-species-specific-real-time-pcr-assay-for-the-detection-of-the-emerging-zoonotic-parasite-ancylostoma-ceylanicum-in-human-stool
#8
Marina Papaiakovou, Nils Pilotte, Jessica R Grant, Rebecca J Traub, Stacey Llewellyn, James S McCarthy, Alejandro J Krolewiecki, Rubén Cimino, Rojelio Mejia, Steven A Williams
BACKGROUND: Molecular-based surveys have indicated that Ancylostoma ceylanicum, a zoonotic hookworm, is likely the second most prevalent hookworm species infecting humans in Asia. Most current PCR-based diagnostic options for the detection of Ancylostoma species target the Internal Transcribed Spacer (ITS) regions of the ribosomal gene cluster. These regions possess a considerable degree of conservation among the species of this genus and this conservation can lead to the misidentification of infecting species or require additional labor for accurate species-level determination...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28687269/chemopreventive-effects-of-a-tamarindus-indica-fruit-extract-against-colon-carcinogenesis-depends-on-the-dietary-cholesterol-levels-in-hamsters
#9
Flavia Martinello, Vinicius Kannen, João José Franco, Bianca Gasparotto, Juliana Yumi Sakita, Atushi Sugohara, Sergio Britto Garcia, Sergio Akira Uyemura
Tamarind has significant antioxidant potential. We showed that tamarind protects hypercholesterolemic hamsters from atherosclerosis. Hypercholesterolemia might increase the risk of colon cancer. We investigated whether tamarind extract modulates the risk of colon cancer in hypercholesterolemic hamsters. Hamsters (n = 64) were given tamarind and a hypercholesterolemic diet for 8 weeks. The groups were the control, tamarind treatment, hypercholesterolemic, and hypercholesterolemic treated with tamarind groups...
July 4, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28668239/association-of-altered-gut-microbiota-composition-with-chronic-urticaria
#10
Edris Nabizadeh, Nima Hosseini Jazani, Morteza Bagheri, Shahram Shahabi
BACKGROUND: An altered gut microbiota composition has recently been linked to some types of allergies. OBJECTIVE: To compare the relative amounts of Akkermansia muciniphila, Clostridium leptum, Faecalibacterium prausnitzii, and Enterobacteriaceae as members of gut microbiota among patients with chronic urticaria (CU) and healthy controls. METHODS: A total of 20 patients with CU and 20 healthy individuals matched by age and sex participated in the study...
July 2017: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/28647465/french-registry-on-acute-st-elevation-and-non-st-elevation-myocardial-infarction-2015-fast-mi-2015-design-and-baseline-data
#11
Loïc Belle, Guillaume Cayla, Yves Cottin, Pierre Coste, Khalife Khalife, Jean-Noël Labèque, Bruno Farah, Thibaut Perret, Patrick Goldstein, Pierre-Yves Gueugniaud, François Braun, Jacques Gauthier, Martine Gilard, Jean-Yves Le Heuzey, Nicolas Naccache, Elodie Drouet, Vincent Bataille, Jean Ferrières, Etienne Puymirat, François Schiele, Tabassome Simon, Nicolas Danchin
BACKGROUND: The FAST-MI programme, consisting of 1-month surveys of patients admitted to hospital for acute myocardial infarction (AMI) in France, has run since 2005. AIM: To gather data on the characteristics, management and outcomes of patients hospitalized for AMI at the end of 2015 in France and to provide comparisons with the previous surveys. METHODS: Consecutive adults with ST-segment elevation myocardial infarction (STEMI) or non-ST-segment-elevation myocardial infarction (NSTEMI) with symptom onset≤48hours were included over a 1-month period, with a possible extension of recruitment for 1 additional month...
June 21, 2017: Archives of Cardiovascular Diseases
https://www.readbyqxmd.com/read/28634574/reducing-viability-bias-in-analysis-of-gut-microbiota-in-preterm-infants-at-risk-of-nec-and-sepsis
#12
Gregory R Young, Darren L Smith, Nicholas D Embleton, Janet E Berrington, Edward C Schwalbe, Stephen P Cummings, Christopher J van der Gast, Clare Lanyon
Necrotising enterocolitis (NEC) and sepsis are serious diseases of preterm infants that can result in feeding intolerance, the need for bowel resection, impaired physiological and neurological development, and high mortality rates. Neonatal healthcare improvements have allowed greater survival rates in preterm infants leading to increased numbers at risk of developing NEC and sepsis. Gut bacteria play a role in protection from or propensity to these conditions and have therefore, been studied extensively using targeted 16S rRNA gene sequencing methods...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28628614/lamphimerus-a-novel-lamp-assay-for-detecting-amphimerus-sp-dna-in-human-stool-samples
#13
William Cevallos, Pedro Fernández-Soto, Manuel Calvopiña, Cristina Fontecha-Cuenca, Hiromu Sugiyama, Megumi Sato, Julio López Abán, Belén Vicente, Antonio Muro
BACKGROUND: Amphimeriasis is a fish-borne disease caused by the liver fluke Amphimerus spp. that has recently been reported as endemic in the tropical Pacific side of Ecuador with a high prevalence in humans and domestic animals. The diagnosis is based on the stool examination to identify parasite eggs, but it lacks sensitivity. Additionally, the morphology of the eggs may be confounded with other liver and intestinal flukes. No immunological or molecular methods have been developed to date...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28619831/stool-dna-test-of-methylated-syndecan-2-for-the-early-detection-of-colorectal-neoplasia
#14
Feng Niu, Jialing Wen, Xinhui Fu, Chujun Li, Rongsong Zhao, Shan Wu, Hao Yu, Xianglin Liu, Xia Zhao, Side Liu, Xinying Wang, Jianping Wang, Hongzhi Zou
BACKGROUND: While the incidence of colorectal cancer (CRC) is steadily increasing, screening for CRC with conventional approaches is not routinely performed in China. Noninvasive screening methods are attractive options to resolve this issue. Syndecan-2 (SDC2) is frequently methylated in CRC. However, the value of a stool test of methylated SDC2 for the detection of CRC is unknown. METHODS: Methylation status of SDC2 was tested in cell lines and 398 colorectal tissue samples, and further evaluated with 497 stool samples, including 196 from CRC patients, 122 from adenoma patients, and 179 from normal individuals, using real-time methylation-specific PCR (qMSP)...
June 15, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28613041/genotyping-horse-epithelial-cells-from-fecal-matter-by-isolation-of-polymerase-chain-reaction-products
#15
Pero Dimsoski
AIM: To show that application of the polymerase chain reaction (PCR) method modified for amplification of a low-copy number DNA samples, ie, the isolation of PCR products (IPCRp), would represent improvement in obtaining genotypes from a fecal DNA compared with previously used genotyping methods. METHODS: The DNA from the horse fecal matter was extracted by modified Qiagen DNA Stool Mini Kit protocol. Following the extraction, the DNA genotypes from fecal samples were obtained by the most powerful PCR amplification method, the IPCRp...
June 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28612148/first-detection-and-genotyping-of-giardia-intestinalis-in-stool-samples-collected-from-children-in-ghazni-province-eastern-afghanistan-and-evaluation-of-the-pcr-assay-in-formalin-fixed-specimens
#16
Anna Lass, Panagiotis Karanis, Krzysztof Korzeniewski
It is estimated that faecal-orally transmitted diseases are common in Afghanistan, as a consequence of poor hygienic standards of life and widespread contamination of water and food with both human and animal faeces. However, there is little information in the literature concerning infections caused by intestinal parasites in the Afghan population. In this study, we report the occurrence of Giardia intestinalis assemblages (A and B) in formalin-fixed stool samples collected from 245 Afghan schoolchildren living in Ghazni Province in eastern Afghanistan...
June 13, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28606969/faecal-microbiota-study-reveals-specific-dysbiosis-in-spondyloarthritis
#17
Maxime Breban, Julien Tap, Ariane Leboime, Roula Said-Nahal, Philippe Langella, Gilles Chiocchia, Jean-Pierre Furet, Harry Sokol
OBJECTIVE: Altered microbiota composition or dysbiosis is suspected to be implicated in the pathogenesis of chronic inflammatory diseases, such as spondyloarthritis (SpA) and rheumatoid arthritis (RA). METHODS: 16S ribosomal RNA gene sequencing was performed on faecal DNA isolated from stool samples in two consecutive cross-sectional cohorts, each comprising three groups of adult volunteers: SpA, RA and healthy controls (HCs). In the second study, HCs comprised a majority of aged-matched siblings of patients with known HLA-B27 status...
June 12, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28596600/detection-of-dna-amplicons-of-polymerase-chain-reaction-using-litmus-test
#18
Dingran Chang, Kha Tram, Ben Li, Qian Feng, Zhifa Shen, Christine H Lee, Bruno J Salena, Yingfu Li
We report on a new colorimetric DNA detection method that takes advantage of the power of polymerase chain reaction (PCR) and the simplicity of the classic litmus test. The strategy makes use of a modified set of primers for PCR to facilitate ensuing manipulations of resultant DNA amplicons: their tagging with urease and immobilization onto magnetic beads. The amplicon/urease-laden beads are then used to hydrolyze urea, resulting in the increase of pH that can be conveniently reported by a pH-sensitive dye...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28588430/gut-microbial-diversity-in-health-and-disease-experience-of-healthy-indian-subjects-and-colon-carcinoma-and-inflammatory-bowel-disease-patients
#19
V Deepak Bamola, Arnab Ghosh, Raj Kishor Kapardar, Banwari Lal, Simrita Cheema, Priyangshu Sarma, Rama Chaudhry
Background: The intestinal microbiota, through complex interactions with the gut mucosa, play a key role in the pathogenesis of colon carcinoma and inflammatory bowel disease (IBD). The disease condition and dietary habits both influence gut microbial diversity. Objective: The aim of this study was to assess the gut microbial profile of healthy subjects and patients with colon carcinoma and IBD. Healthy subjects included 'Indian vegetarians/lactovegetarians', who eat plant produce, milk and milk products, and 'Indian non-vegetarians', who eat plant produce, milk and milk products, certain meats and fish, and the eggs of certain birds and fish...
2017: Microbial Ecology in Health and Disease
https://www.readbyqxmd.com/read/28579087/the-protective-role-of-helicobacter-pylori-neutrophil-activating-protein-in-childhood-asthma
#20
A Karakullukcu, H B Tokman, S Nepesov, M Demirci, S Saribas, S Vehid, R Caliskan, Z Taner, H Cokugras, T Ziver, S Demiryas, B Kocazeybek
BACKGROUND: Helicobacter pylori quantity and HP-NAP gene expression were evaluated in the faeces of healthy and asthmatic children. METHODS: H. pylori DNAs and RNAs were isolated from the stool samples of 92 asthmatic children (AC; 3-8 years) and 88 healthy controls (HC). Quantitative PCR was used to determine the quantity of H. pylori and HP-NAP expression relative to the 16S rRNA (reference gene). Gene expression was analysed using the delta delta-Ct method. RESULTS: H...
May 31, 2017: Allergologia et Immunopathologia
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