Modified ultrafiltration

Elimination of tiny oil droplets nearly miscible with wastewater can be realized using membrane technology through ultrafiltration. The novelty of this work was to blend different phases of molybdenum disulfide (MoS2 ) in isotropic polyethersulfone (PES). We prepared isotropic PES membranes by optimizing nonsolvent vapour-induced phase separation (VIPS). Membranes were blended with MoS2 nanosheets of different phases to promote separation performance and antifouling resistance. FE-SEM revealed the flower-like surface morphology of MoS2 nanosheets...

Surface modification is an attractive strategy to adjust the properties of polymer membranes. Unfortunately, predictive structure-processing-property relationships between the modification strategies and membrane performance are often unknown. One possibility to tackle this challenge is the application of data-driven methods such as machine learning. In this study, we applied machine learning methods to data sets containing the performance parameters of modified membranes. The resulting machine learning models were used to predict performance parameters, such as the pure water permeability and the zeta potential of membranes modified with new substances...

Plants can produce complex pharmaceutical and technical proteins. Spider silk proteins are one example of the latter and can be used, for example, as compounds for high-performance textiles or wound dressings. If genetically fused to elastin-like polypeptides (ELPs), the silk proteins can be reversibly precipitated from clarified plant extracts at moderate temperatures of ~ 30 °C together with salt concentrations > 1.5 M, which simplifies purification and thus reduces costs...

Global water scarcity is a threat that can be alleviated through membrane filtration technologies. However, the widespread adoption of membranes faces significant challenges, primarily due to membrane biofouling. This is the reason why membrane modifications have been under increasing investigation to address the fouling issues. Antibacterial membranes, designed to combat biofouling by eliminating microorganisms, offer a promising solution. Within this study, flat sheet ultrafiltration (UF) membranes with integrated photocatalytic zinc oxide (ZnO) nanoparticles were developed, characterized, and assessed through filtration and fouling tests...

Contamination of groundwater by nitrate from intensive agriculture is a serious problem globally. Excessive fertilization has led to nitrate contamination of the Coastal Aquifer in Israel. Here we report the efficient removal of nitrate from contaminated groundwater by micellar-enhanced ultrafiltration (MEUF) using a specially tailored membrane. Graft polymerization with hydrophilic poly(methacrylate) and incorporation of porous zeolitic imidazole framework ZIF-L nanoparticles imparted antifouling properties to the membrane...

Abandoning the costly development of new membrane materials and instead directly remodeling the naturally occurring cake layer constitutes a dynamic, low-cost, long-lasting, and proactive strategy to "fight fouling with fouling". Several optimization strategies, including coagulation/modified magnetic seed loading and applying a weak magnetic force (0.01T) at the ultrafiltration end, improved the anti-fouling, retention, and sieving performances of conventional ultrafiltration process during the treatment of source water having complex natural organic matter (NOMs) and small molecule micropollutants...

Polymeric membranes have garnered great interest in wastewater treatment; however, fouling is known as their main limitation. Therefore, the blending of hydrophilic nanoparticles in polymeric membranes' structure is a promising approach for fouling reduction. Herein, a hydrophilic boehmite-tannic acid-graphene quantum dot (BM-TA-GQD) nanoparticle was synthesized and blended in a polyethersulfone polymeric membrane in different percentages. The fabricated membranes were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) images, water contact angle, porosity measurement, and antibacterial and antifouling properties...

Reducing sugars causes confirmatory alterations in albumin structure by the nonenzymatic glycation of the amino group of serum albumin. In this study, glucose and its hazardous metabolic products like glyoxal and methylglyoxal were incubated with bovine serum albumin (BSA). The confirmational changes in BSA molecule's structure by glycating substances was investigated using a variety of spectroscopic methods, including deconvolutionFourier Transform Infra-red (FT-IR) spectroscopy, fluorescence spectroscopy, UV spectroscopy and circular dichroism (CD) spectroscopy...

PURPOSE OF REVIEW: To summarize the contemporary evidence on decongestion strategies in patients with acute heart failure (AHF). RECENT FINDINGS: While loop diuretic therapy has remained the backbone of decongestive treatment in AHF, multiple randomized clinical trials suggest that early combination with other diuretic classes or molecules with diuretic properties should be considered. Mineralocorticoid receptor antagonists and sodium-glucose co-transporter-2 inhibitors are disease-modifying drugs in heart failure that favourably influence prognosis early on, advocating their start as soon as possible in the absence of any compelling contraindications...

Ultralow-pressurized ultrafiltration membrane process with low energy consumption is promising in surface water purification. However, membrane fouling and low selectivity are significant barriers for the wide application of this process. Herein, an ultrathin zwitterionic hydrogel nanolayer was in-situ grown on polysulfone ultrafiltration membrane surface through interfacially-initiated free radical polymerization. The hydrogel-modified membrane possessed improved biological fouling resistance during the dynamic filtration process (bovine serum albumin, Escherichia coli and Staphylococcus aureus), comparing with commercial polysulfone membrane...

Exosomes are bioactive substances secreted by various cells that play a crucial role in cell communication. Due to their nanoscale size and interference from nonexosome proteins, the rapid capture and nondestructive release of exosomes remain a technical challenge which significantly hinders their biomedical application. To overcome this obstacle, we have designed a CD63 aptamer-based thermosensitive copolymer for the effective isolation of exosomes from mesenchymal stem cells (MSCs). A thermal-responsive copolymer, poly( N -isopropylacrylamide- co -butyl methacrylate- co - N -hydroxysuccinimide methacrylate) P(NIPAM- co -BMA- co -NHSMA, PNB), was prepared, which could realize reversible hydrophilic/hydrophobic phase transition by varying temperature...

Sodium hypochlorite (NaClO) is widely used for the chemical cleaning of fouled ultrafiltration (UF) membranes. Various studies performed on polymeric membranes demonstrate that long-term (>100 h) exposure to NaClO deteriorates the physicochemical properties of the membranes, leading to reduced performance and service life. However, the effect of NaClO cleaning on ceramic membranes, particularly the number of cleaning cycles they can undergo to alleviate irreversible fouling, remains poorly understood. Silicon carbide (SiC) membranes have garnered widespread attention for water and wastewater treatment, but their chemical stability in NaClO has not been studied...

Pulse proteins, such as pea and chickpea proteins, have inferior functionality, specifically gelation, compared to soy protein, hindering their applications in different food products, such as meat analogs. To close the functionality gap, protein polymerization via targeted modification can be pursued. Accordingly, transglutaminase-induced polymerization was evaluated in pea protein isolate (PPI) and chickpea protein isolate (ChPI) to improve their functionality. The PPI and ChPI were produced following a scaled-up salt extraction coupled with ultrafiltration (SE-UF) process...

This study is an extension of our previous studies in which the lysozyme was isolated and purified from Bacillus subtilis BSN314 (Naveed et al., 2022; Naveed et al., 2023). In this study, the lysozyme genes were cloned into the E. coli BL21. For the expression of lysozyme in E. coli BL21, two target genes, Lyz-1 and Lyz-2, were ligated into the modified vector pET28a to generate pET28a-Lyz1 and pET28a-Lyz2, respectively. To increase the production rate of the enzyme, 0.5-mM concentration of IPTG was added to the culture media and incubated at 37 °C and 220 rpm for 24 h...

Host cell protein (HCP) characterization is a crucial quality parameter for biotherapeutic drug safety and stability. With a liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach, we identified ubiquitin in ultrafiltration/diafiltration (UF/DF) pools of one of our monoclonal antibody (mAb) products. Since ubiquitin occurs physiologically as a post-translational modification (PTM) involved in many cellular functions, we suspected the possibility that if identified as an HCP, it may occur as a covalent modification on the mAb...

INTRODUCTION: Heparin is the primary anticoagulant for cardiopulmonary bypass (CPB) support during cardiac surgery. While widely used, ∼2% of cardiac surgery patients develop heparin-induced thrombocytopenia (HIT) and 4-26% develop heparin resistance. Bivalirudin is an alternative anticoagulant mainly used for percutaneous coronary interventions. Given the challenges associated with heparin anticoagulation, we conducted a review to explore the use of bivalirudin for CPB surgery...

INTRODUCTION: Smoking is common in chronic hemodialysis patients who are characterized by a high cardiovascular risk. PATIENTS & METHODS: This is a multicenter cross-sectional study over a period of 4 months, from July 2021 to October 2021, in the Agadir province. RESULTS: Among the 245 patients interviewed, 9,4% were current smokers and 16,7% were former smokers. Compared to non-smoking patients, former and current smokers were predominantly male, had more comorbidities, higher diastolic blood pressure, higher mean ultrafiltration rate and more antihypertensive treatments...

A newly developed water-soluble polymeric nano-additive termed "partially cross-linked nanoparticles graft copolymer (PCLNPG)" has been successfully synthesized and harnessed as a pore former for modifying a polyethersulfone ultrafiltration membrane for dyes removal. The PCLNPG content was varied in the PES polymeric matrix aiming to scrutinize its impact on membrane surface characteristics, morphological structure, and overall performance. Proposed interaction mechanism between methylene blue (MB), methyle orange (MO), and malachite green (MG) dyes with PES membrane was presented as well...

Abstract In this work, a new type of composite nanoparticles, "pearl chain", was developed by linking titanium dioxide and silicon dioxide by polyacrylic acid polymer chains, and the prepared TiO2 -PAA-SiO2 composite nanoparticles were analyzed by SEM, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy and thermogravimetric analysis, zeta potential, x-ray diffraction, etc. The success of this work was verified by the successful linking of TiO2 -PAA-SiO2 composite nanoparticles.TiO2 -PAA-SiO2 composite nanoparticles were analyzed to verify the successful attachment of pearl chains...

To mitigate membrane fouling and address the trade-off between permeability and selectivity, we fabricated nanocellulose (NC) fine-tuned polyvinylidene fluoride (PVDF) porous membranes (NC-PVDFs) using phase inversion method through blending NCs with varied aspect ratios, surface charges and grafted functional groups. NC-PVDF presented rougher surface (increased by at least 18.3 %), higher porosity and crystallinity compared to PVDF membrane. Moreover, cellulose nanocrystals incorporated PVDF (CNC-PVDF) elevated membrane surface charge and hydrophilicity (from 74...