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https://www.readbyqxmd.com/read/28734144/expression-of-planar-cell-polarity-genes-during-mouse-tooth-development
#1
Nobuko Obara, Yuko Suzuki, Kazuharu Irie, Shunichi Shibata
OBJECTIVE: Planar cell polarity (PCP) refers to the cell polarity across the tissue plane and controls various cell behaviors and structures. Although the expression of several PCP signaling components has been detected in tooth germs, knowledge of the gene expression patterns of these PCP components during tooth development remains incomplete. The aim of this study is to characterize the temporal and spatial changes in PCP gene expression during tooth development. DESIGN: Expression of Celsr1 and 2, Fzd3 and 6, Vangl1 and 2, and Dvl1-3 genes was analyzed in mouse molar germs from the bud to the bell stage using in situ hybridization...
July 14, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28734061/evaluation-of-a-technique-for-color-correction-in-restoring-anterior-teeth
#2
Gabrielle Branco Rauber, Jussara Karina Bernardon, Luiz Clovis Cardoso Vieira, Luiz Narciso Baratieri
OBJECTIVE: This study aimed to evaluate the ability of the proposed technique in producing restorations that exhibit mimesis with tooth structure and to define a restorative clinical protocol. MATERIALS AND METHODS: For this study a typodont was used. The right upper central incisor with Class IV lesion was restored with the layering technique (reference tooth, RT). For the left upper central incisor with Class IV lesion, six teeth were restored monochromatically (test teeth, TT), using DA3...
July 22, 2017: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/28733464/lineage-tracing-of-epithelial-cells-in-developing-teeth-reveals-two-strategies-for-building-signaling-centers
#3
Wei Du, Jimmy Kuang-Hsien Hu, Wen Du, Ophir D Klein
An important event in organogenesis is the formation of signaling centers, which are clusters of growth-factor secreting cells. In the case of tooth development, sequentially formed signaling centers known as the initiation knot (IK) and the enamel knot (EK) regulate morphogenesis. However, despite the importance of signaling centers, their origin, as well as the fate of the cells composing them, remain open questions. Here, using lineage tracing of distinct epithelial populations, we found that the EK of the mouse incisor is derived de novo from a group of SRY-box 2 (Sox2)-expressing cells in the posterior half of the tooth germ...
July 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28732182/stim1-regulates-enamel-mineralization-and-ameloblast-modulation
#4
Y Furukawa, N Haruyama, M Nikaido, M Nakanishi, N Ryu, M Oh-Hora, K Kuremoto, K Yoshizaki, Y Takano, I Takahashi
Loss-of-function mutations in the Ca(2+) release-activated Ca(2+) channel genes ORAI1 and STIM1 abolish store-operated Ca(2+) entry (SOCE) and result in ectodermal dysplasia with amelogenesis imperfecta. However, because of the limited availability of patient tissue, analyses of enamel mineralization or possible changes in ameloblast function or morphology have not been possible. Here, we generated mice with ectodermal tissue-specific deletion of Stim1 ( Stim1 cKO [conditional knockout]), Stim2 ( Stim2 cKO), and Stim1 and Stim2 ( Stim1/2 cKO) and analyzed their enamel phenotypes as compared with those of control ( Stim1/2(fl/fl)) animals...
July 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28731486/demineralized-dentin-and-enamel-matrices-as-suitable-substrates-for-bone-regeneration
#5
Nina Bono, Paolo Tarsini, Gabriele Candiani
BACKGROUND: In recent decades, tooth derivatives such as dentin (D) and enamel (E) have been considered as potential graft biomaterials to treat bone defects. This study aimed to investigate the effects of demineralization on the physical-chemical and biological behavior of D and E. METHODS: Human D and E were minced into particles (Ø<1 mm), demineralized and sterilized. Thorough physical-chemical and biochemical characterizations of native and demineralized materials were performed by SEM and EDS analysis and ELISA kits to determine mineral, collagen type I and BMP-2 contents...
July 20, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28729789/in-vitro-evaluation-of-microleakage-under-orthodontic-brackets-bonded-with-different-adhesive-systems
#6
Ramin Atash, Ali Fneiche, Sibel Cetik, Babak Bahrami, Alain Balon-Perin, Maria Orellana, Régine Glineur
OBJECTIVE: Adhesives systems have a drawback when utilized for bonding orthodontic brackets: they shrink during photopolymerization creating microleakage. The aim of this study was to assess the stability of different orthodontic adhesives around brackets and enamel. MATERIALS AND METHODS: Sixty noncarious mandibular premolars extracted for orthodontic reasons were randomly divided into six groups of adhesives used for bonding brackets to dental enamel: NeoBond(®) Light Cure Adhesive Kit, Transbond™ Plus Self-Etching, Victory V-Slot APC PLUS(®) + Transbond™ MIP, Rely-A-Bond(®) Kit, Light Cure Orthodontic Adhesive Kit (OptiBond(®)), and Transbond™ MIP...
April 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28729668/novel-fam83h-mutations-in-patients-with-amelogenesis-imperfecta
#7
Wang Xin, Wang Wenjun, Qin Man, Zhao Yuming
Amelogenesis imperfecta (AI), characterized by a deficiency in the quantity and/or quality of dental enamel, is genetically heterogeneous and phenotypically variable. The most severe type, hypocalcified AI, is mostly caused by truncating mutations in the FAM83H gene. This study aimed to identify genetic mutations in four Chinese families with hypocalcified AI. We performed mutation analysis by sequencing the candidate FAM83H gene. Three novel mutations (c.931dupC, p.V311Rfs*13; c.1130_1131delinsAA, p.S377X; and c...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729563/effect-of-resin-infiltration-on-the-color-and-microhardness-of-bleached-white-spot-lesions-in-bovine-enamel-an-in-vitro-study
#8
(no author information available yet)
This study would appear to give little steer as to which of the many approaches most effectively manage white spot lesions.
July 21, 2017: British Dental Journal
https://www.readbyqxmd.com/read/28727472/relieving-dry-mouth-varying-levels-of-ph-found-in-bottled-water
#9
Bailey Jean Fisher, Angela Spencer, Van Haywood, Gayathri Konchady
It is estimated that 30% of people older than 60 years suffer from hyposalivation or dry mouth. Drinking water frequently has been recommended as a safe, non-pharmacologic way to combat hyposalivation. The saliva in patients with dry mouth is acidic. Beverages consumed daily may have an erosive potential on teeth. The pH and the mineral content of the beverage determine its erosive potential. An acidic beverage, therefore, may have harmful effects on mineralized tooth structures, causing erosion of enamel, dentin, and cementum...
July 2017: Compendium of Continuing Education in Dentistry
https://www.readbyqxmd.com/read/28727410/exosomes-mediate-epithelium-mesenchyme-crosstalk-in-organ-development
#10
Nan Jiang, Lusai Xiang, Ling He, Guodong Yang, Jinxuan Zheng, Chenglin Wang, Yimei Zhang, Sainan Wang, Yue Zhou, Tzong-Jen Sheu, Jiaqian Wu, Kenian Chen, Paulo G Coelho, Nicky M Tovar, Shin Hye Kim, Mo Chen, Yan-Heng Zhou, Jeremy J Mao
Organ development requires complex signaling by cells in different tissues. Epithelium and mesenchyme interactions are crucial for the development of the skin, hair follicles, kidney, lungs, prostate, major glands and the teeth. Despite myriad literature on cell-cell interactions and ligand-receptor binding, the roles extracellular vesicles in epithelium-mesenchyme interactions during organogenesis are poorly understood. Here, we discovered that ~100 nm exosomes were secreted by the epithelium and mesenchyme of a developing tooth organ, and diffused through the basement membrane...
July 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/28727319/novel-evaluation-and-treatment-techniques-for-white-spot-lesions-an-in-vitro-study
#11
T A Bakhsh, A S Bakry, M M Mandurah, M A Abbassy
OBJECTIVE: White spot lesions (WSLs) are commonly seen during and after orthodontic treatment. Therefore, the objective of this in vitro study was to assess the effect of 45S5-bioglass in remineralizing WSLs using cross-polarization optical coherence tomography (CP-OCT) and confirm the findings by micro-hardness test. METHODS: Ceramic orthodontic brackets were bonded to the buccal surface of 45 human premolars with Transbond XT primer followed by Transbond PLUS according to the manufacturer's instructions...
August 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28727170/entire-papilla-preservation-technique-in-the-regenerative-treatment-of-deep-intrabony-defects-1-year-results
#12
Serhat Aslan, Nurcan Buduneli, Pierpaolo Cortellini
AIM: This study evaluates the clinical performance of a novel tunnel-like surgical technique in the treatment of isolated deep intrabony defects. MATERIAL AND METHODS: Twelve patients presenting with at least one isolated deep intrabony defect received regenerative periodontal treatment with "entire papilla preservation" technique. Access to the intrabony defect for debridement was provided by a bevelled vertical releasing incision positioned in the buccal gingiva of the neighbouring interdental space...
July 20, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28722001/enamel-color-changes-following-orthodontic-treatment
#13
REVIEW
Akshaya Pandian, Sukanya Ranganathan, Sridevi Padmanabhan
OBJECTIVE: To evaluate and compare the effect of various orthodontic bonding systems and clean up procedures on quantitative enamel colour change. MATERIALS AND METHODS: A literature search was done to identify the studies that assessed the quantitative enamel colour change associated with the various bonding systems and cleanup procedures. Electronic database (Pub Med, Cochrane and Google Scholar) were searched. First stage screening was performed and the abstracts were selected according to the initial selection criteria...
May 2017: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
https://www.readbyqxmd.com/read/28721667/molar-incisor-hypomineralisation-mih-training-manual-for-clinical-field-surveys-and-practice
#14
A Ghanim, M J Silva, M E C Elfrink, N A Lygidakis, R J Mariño, K L Weerheijm, D J Manton
BACKGROUND: Despite clear assessment criteria, studies of molar incisor hypomineralisation (MIH) and hypomineralised second primary molars (HSPM) are marked by inconsistency in outcome measurements. This has detracted from meaningful comparisons between studies and limited interpretation. AIM: To provide a comprehensive manual as a companion to assist researchers in planning epidemiological studies of MIH and HSPM, with particular reference to outcome measurement...
July 18, 2017: European Archives of Paediatric Dentistry: Official Journal of the European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/28718921/homology-homoplasy-and-cusp-variability-at-the-enamel-dentine-junction-of-hominoid-molars
#15
Alejandra Ortiz, Shara E Bailey, Jean-Jacques Hublin, Matthew M Skinner
Evolutionary studies of mammalian teeth have generally concentrated on the adaptive and functional significance of dental features, whereas the role of development on phenotypic generation and as a source of variation has received comparatively little attention. The present study combines an evolutionary biological framework with state-of-the-art imaging techniques to examine the developmental basis of variation of accessory cusps. Scholars have long used the position and relatedness of cusps to other crown structures as a criterion for differentiating between developmentally homologous and homoplastic features, which can be evaluated with greater accuracy at the enamel-dentine junction (EDJ)...
July 18, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28717800/from-molecules-to-macrostructures-recent-development-of-bioinspired-hard-tissue-repair
#16
REVIEW
Chunmei Ding, Zhuoxin Chen, Jianshu Li
Enamel, dentin and bone are calcified hard tissues in the human body that play significant roles in food mastication and movement support. Generally, hard tissues lack the ability of self-repair, except for the regeneration ability of bone for small-scale defects. Fabrication of man-made repair materials is therefore highly demanded. In this review, following a bioinspired strategy, we describe the composition and multiscale structures of different hard tissues, and highlight the key points for the reconstruction of hard tissues...
July 18, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28717632/effect-of-different-intracoronal-bleaching-methods-on-shear-bond-strength-of-ceramic-brackets-bonded-to-bleached-enamel-an-in-vitro-study
#17
Vikas Chauhan, Piush Kumar, Payal Sharma, Divya Shetty
OBJECTIVE: To investigate the effect of different intracoronal bleaching methods on the shear bond strength and site of failure of ceramic brackets. MATERIALS AND METHODS: Sixty freshly extracted human maxillary incisors were randomly divided into four groups (n = 15). Endodontic access cavity was prepared and root canals were filled, root fillings were removed 2mm apical to the cementoenamel junction, and a 2-mmthick layer of glass ionomer cement base was applied...
July 2017: Journal of Orthodontic Science
https://www.readbyqxmd.com/read/28715508/biofilm-extracellular-polysaccharides-degradation-during-starvation-and-enamel-demineralization
#18
Bárbara Emanoele Costa Oliveira, Jaime Aparecido Cury, Antônio Pedro Ricomini Filho
This study was conducted to evaluate if extracellular polysaccharides (EPS) are used by Streptococcus mutans (Sm) biofilm during night starvation, contributing to enamel demineralization increasing occurred during daily sugar exposure. Sm biofilms were formed during 5 days on bovine enamel slabs of known surface hardness (SH). The biofilms were exposed to sucrose 10% or glucose + fructose 10.5% (carbohydrates that differ on EPS formation), 8x/day but were maintained in starvation during the night. Biofilm samples were harvested during two moments, on the end of the 4th day and in the morning of the 5th day, conditions of sugar abundance and starvation, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28713769/fracture-fragment-reattachment-using-projectors-and-anatomic-everstick-post%C3%A2-an-ultraconservative-approach
#19
Velagala L Deepa, Satti Narayana Reddy, Venkata Charan Garapati, Srirama Rao Sudhamashetty, Padmasri Yadla
Traumatized anterior teeth require quick functional and esthetic repair. Tooth fragment reattachment is a simple and conservative clinical procedure to restore fractured anterior teeth. Different techniques of reattachment include enamel beveling, "v-" shaped internal enamel and dentin grooves, external chamfer, over contouring, and by placing fiber post, all of which were invasive methods. This article describes a novel technique of reattachment using canal projectors for stabilizing fractured fragments followed by endodontic therapy and internal reinforcement with custom fiber post (everStick Post™, GC Corporation, Tokyo, Japan)...
June 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28713645/effects-of-a-novel-disc-formulation-on-dry-mouth-symptoms-and-enamel-remineralization-in-patients-with-hyposalivation-an-in-vivo-study
#20
Jessica Ho, Miracle Vania Firmalino, Afarin Golabgir Anbarani, Thair Takesh, Joel Epstein, Petra Wilder-Smith
BACKGROUND: The goal of this study was to identify the in vivo effects in patients with hyposalivation of a novel slowly dissolving adhering test disc upon on enamel remineralization, oral biofilm, salivary production, pH and buffering, gingival health, and on self-evaluation of oral well-being. METHODS: Five subjects with xerostomia wore custom made retainers carrying 5 demineralized enamel chips for periods of 1 week each. In 1 study arm, subjects used the test agent plus oral hygiene self-care; in the other they used oral hygiene self-care only, with a 1 week washout in between arms...
February 2017: Dentistry: Current Research
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