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Decalcification

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https://www.readbyqxmd.com/read/29157956/comparative-analysis-of-fixation-and-embedding-techniques-for-optimized-histological-preparation-of-zebrafish
#1
Jean E Copper, Lynn R Budgeon, Christina A Foutz, Damian B van Rossum, Daniel J Vanselow, Margaret J Hubley, Darin P Clark, David T Mandrell, Keith C Cheng
In recognition of the importance of zebrafish as a model organism for studying human disease, we have created a web-based reference atlas of zebrafish microanatomy for comparing histology and histopathology between model systems and with humans (http://bio-atlas.psu.edu). Fixation, decalcification, embedding, and sectioning were optimized to maximize section quality. A comparison of protocols involving six fixatives showed that 10% Neutral Buffered Formalin at 21°C for 24h yielded excellent results. Sectioning of juveniles and adults requires bone decalcification; EDTA at 0...
November 17, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29131649/characterization-of-proteomes-extracted-through-collagen-based-stable-isotope-and-radiocarbon-dating-methods
#2
Caroline Wadsworth, Michael Buckley
Isotope analyses on "collagen" extracted from ancient bone have been routinely used for dietary and chronological inferences worldwide for decades. These methods involve the decalcification of biomineralized tissues with acid, often followed by processes to remove exogenous contaminants, and then gelatinization of what is often described as the "collagen" fraction. However, little is known about the relative content of collagen to the many other noncollagenous proteins (NCPs) potentially present. Some of these NCPs have great longevity in ancient bone, and some, for example, fetuin-A, are useful for obtaining better taxonomic information than collagen...
November 13, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29110340/effect-of-topical-fluoride-application-on-enamel-after-in-office-bleaching-as-evaluated-using-a-novel-hardness-tester-and-a-transverse-microradiography-method
#3
Mai Fukuyama, Chiharu Kawamoto, Pipop Saikaew, Yasuhiro Matsuda, Ricardo M Carvalho, Denis Selimovic, Hidehiko Sano
This study evaluated the effect of topical fluoride application on enamel hardness after in-office bleaching. Twelve human incisors were cut along the long axis, resulting in 24 halves used in four treatment groups (n = 6 in each group): (i) untreated group (C); (ii) in-office bleaching material (B); (iii) treatment with surface reaction-type prereacted glass-ionomer varnish after in-office bleaching (B+PRG); and (iv) treatment with acidulated phosphate fluoride solution after bleaching (B+F). All specimens were subjected to pH-cycling for 4 wk...
November 7, 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/29076527/a-comparative-analysis-of-microscopic-alterations-in-modern-and-ancient-undecalcified-and-decalcified-dry-bones
#4
Valentina Caruso, Marco Cummaudo, Emanuela Maderna, Annalisa Cappella, Giorgio Caudullo, Valentina Scarpulla, Cristina Cattaneo
OBJECTIVES: The present study aims to evaluate the preservation of the microstructure of skeletal remains collected from four different known burial sites (archaeological and contemporary). Histological analysis on undecalcified and decalcified thin sections was performed in order to assess which of the two techniques is more affected by taphonomic insults. MATERIALS AND METHODS: A histological analysis was performed on both undecalcified and decalcified thin sections of 40 long bones and the degree of diagenetic change was evaluated using transmitted and polarized light microscopy according to the Oxford Histological Index (OHI)...
October 27, 2017: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/29023217/visual-impairment-by-multiple-vascular-embolization-with-hydroxyapatite-particles
#5
Yayoi Marumo, Miki Hiraoka, Masato Hashimoto, Hiroshi Ohguro
We demonstrate a case of ocular impairment caused by a hydroxyapatite filler injection and review the prior literature on clinical presentations. A healthy woman, who received a hydroxyapatite filler injection into the glabella for nose augmentation suddenly had symptoms of nausea, diplopia, visual loss in the left eye, and impaired consciousness. Her left eye showed paresis of the inferior branch of the oculomotor nerve, conjunctival injection, cell infiltration in the anterior chamber, and multiple white spots in the nasal fundus...
October 12, 2017: Orbit
https://www.readbyqxmd.com/read/28958188/comparison-of-different-decalcification-methods-using-rat-mandibles-as-a-model
#6
Flavia M Savi, Gary I Brierly, Jeremy Baldwin, Christina Theodoropoulos, Maria A Woodruff
Selection of decalcification agents is an essential consideration when processing mineralized tissues because the integrity and immunohistochemical characteristics of the tissues may be affected. Here, we report results obtained from the decalcification of rat mandibles using 10% ethylenediaminetetraacetic acid (EDTA) at room temperature (RT), 10% EDTA at 37C, 5% nitric acid, and 10% formic acid at RT. Decalcification endpoints were determined by microcomputed tomography. Morphological preservation and antigenicity were evaluated by hematoxylin and eosin staining and immunohistochemistry...
September 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28929330/development-of-a-rapid-analysis-method-for-bone-resection-margins-for-oral-squamous-cell-carcinoma-by-immunoblotting
#7
Claudia Haase, Bernd Lethaus, Ruth Knüchel-Clarke, Frank Hölzle, Angela Cassataro, Till Braunschweig
The purpose of this proof-of-principle study was to develop a rapid and approachable method to analyse bone resection margins in patients with oral squamous cell carcinoma (OSCC) in an intraoperative setting, similar to assessing frozen sections of soft tissue. Bone excision and risk of remaining tumour cells could be minimised, thus improving reconstruction measures and facilitating convalescence. Frozen, sawed wafers of porcine bone artificially combined with porcine skin (simulating OSCC properties) were used to develop and evaluate a new molecular method: protein transfer from non-decalcified, sawed wafers onto a membrane stained by immunofluorescence (Tissue-ProtTrans)...
September 19, 2017: Head and Neck Pathology
https://www.readbyqxmd.com/read/28920546/blood-supply-and-vascularity-of-the-glenoid-labrum-its-clinical-implications
#8
Abduelmenem Alashkham, Abdulrahman Alraddadi, Paul Felts, Roger Soames
BACKGROUND: Tears of the glenoid labrum are common after dislocation of the glenohumeral joint. The outcome for healing or surgical reconstruction of the glenoid labrum relies on the extent of its vascularization. This study aims to evaluate the glenoid labrum blood supply and to determine its regional vascularity. MATERIALS AND METHODS: A total of 140 shoulders (30 male and 40 female cadavers) were examined: mean age 81.5 years, range 53-101 years. All blood vessels around the glenohumeral joint were dissected and recorded...
September 2017: Journal of Orthopaedic Surgery
https://www.readbyqxmd.com/read/28915544/mobilisation-of-toxic-trace-elements-under-various-beach-nourishments
#9
Iris R Pit, Stefan C Dekker, Tobias J Kanters, Martin J Wassen, Jasper Griffioen
To enhance protection and maintain wide beaches for recreation, beaches are replenished with sand: so-called beach nourishments. We compared four sites: two traditional beach nourishments, a mega beach nourishment and a reference without beach nourishment. Two sites contain calcareous-rich sand, whereas the other two sites have calcareous-poor sand. We aimed to understand hydrogeochemical processes to indicate factors critical for the mobility of trace elements at nourishments. We therefore analysed the chemical characteristics of sediment and pore water to ascertain the main drivers that mobilise toxic trace elements...
December 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28890234/calibration-of-a-lactic-acid-model-for-simulating-biofilm-induced-degradation-of-the-dentin-composite-interface
#10
Laikuan Zhu, Yuping Li, Carola A Carrera, Yung-Chung Chen, Mingyu Li, Alex Fok
OBJECTIVE: To verify and calibrate a chemical model for simulating the degradation of the dentin-composite interface induced by multi-species oral biofilms in vitro. METHODS: Dentin-composite disks (5-mm dia.×2-mm thick) were made from bovine incisor roots and filled with either Z100™ (Z100) or Filtek™ LS (LS) composite. The disks, which were covered with nail varnish, but with one of the dentin-composite margins exposed, were immersed in lactic acid solution at pH 4...
November 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28877070/effects-of-hydrochloric-acid-and-formic-acid-decalcification-on-breast-tumor-biomarkers-and-her2-fluorescence-in-situ-hybridization
#11
Beth Z Clark, Jennifer M Yoest, Agnieszka Onisko, David J Dabbs
Biomarker analysis of metastatic breast carcinoma (MBC) is routinely recommended by ASCO/CAP guidelines, and establishing a diagnosis of MBC often requires immunohistochemistry (IHC). The reliability of breast tumor biomarkers and breast-specific markers on decalcified tissues has not been extensively studied. We performed IHC studies on breast tumors exposed to hydrochloric acid (HCl) and formic acid (FA) decalcification solutions, and HER2 fluorescence in situ hybridization on a subset of these tumors to establish a protocol for handling bone specimens with suspicion for MBC...
September 4, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28873437/three-dimensional-hard-and-soft-tissue-imaging-of-the-human-cochlea-by-scanning-laser-optical-tomography-slot
#12
Nadine Tinne, Georgios C Antonopoulos, Saleh Mohebbi, José Andrade, Lena Nolte, Heiko Meyer, Alexander Heisterkamp, Omid Majdani, Tammo Ripken
The present study focuses on the application of scanning laser optical tomography (SLOT) for visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique which allows for tomographic imaging of the internal structure of transparent specimens. Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises decalcification, dehydration as well as optical clearing of the cochlea samples in toto...
2017: PloS One
https://www.readbyqxmd.com/read/28864350/immunohistochemical-analysis-of-nkx2-2-etv4-and-bcor-in-a-large-series-of-genetically-confirmed-ewing-sarcoma-family-of-tumors
#13
Isidro Machado, Akihiko Yoshida, José Antonio López-Guerrero, María Gema Nieto, Samuel Navarro, Piero Picci, Antonio Llombart-Bosch
Ewing sarcoma is an aggressive neoplasm of pediatric and adolescent patients. Immunohistochemistry (IHC) can be used to support the morphologic diagnosis of Ewing sarcoma family of tumors (ESFT) in a convincing clinical/radiological context. Although neither NKX2.2 nor CD99 alone are entirely specific, when combined, the diagnostic specificity is high. The aim of the present study was to investigate the IHC expression of NKX2.2, ETV4 and BCOR in a large series of genetically confirmed ESFT. The results for CD99 and CAV-1 immunoreactivity, and the histological and fusion gene subtypes were retrieved from our previous study...
August 25, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28856324/decalcification-of-a-heavily-calcified-common-femoral-artery-and-its-bifurcation-with-a-cavitron-ultrasonic-surgical-aspirator
#14
S Maeda, T Nakamura
INTRODUCTION: Surgical endarterectomy is the preferred method for treating occlusive disease of the common femoral artery (CFA). However, endarterectomy is not always straightforward in cases with heavily calcified plaque. To overcome this limitation, a new method for decalcification, which utilizes a Cavitron ultrasonic surgical aspirator (CUSA) has been developed. REPORT: The method involves full exposure of the calcified lesion. Following an arteriotomy, protruding calcification is removed using the CUSA, taking care to avoid vessel perforation...
2017: EJVES Short Rep
https://www.readbyqxmd.com/read/28833664/compound-specific-amino-acid-%C3%AE-15-n-values-in-archaeological-shell-assessing-diagenetic-integrity-and-potential-for-isotopic-baseline-reconstruction
#15
Nicole Misarti, Elizabeth Gier, Bruce Finney, Kelli Barnes, Matthew McCarthy
RATIONALE: Reconstructing stable isotope (SI) ratios at the base of paleo-food webs is often challenging. For coastal systems, the SI ratios of organic matter in archeological shell represents a possible solution, providing a direct record of primary consumer SI ratios in the littoral zone. However, shell is often porous, with organic compounds susceptible to diagenetic alteration or contamination. If molecular isotopic information is well preserved, compound-specific amino acid isotope analysis (CSI-AA) has the potential to provide direct proxies for baseline SI ratios, bypassing many contamination issues, and to allow assessment of the diagenetic state...
November 30, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28810293/-comparative-study-of-three-different-decalcifying-solutions-in-bone-metastasis-specimens-with-breast-cancer
#16
H Y Wu, T Wang, K L Chen, Z W Fan, X H Pu, B Zhang, X S Fan, F Q Meng
Objective: To investigate the optimal strategy for immunohistochemical (IHC) staining in bone metastasis specimens from breast cancer. Methods: Twenty-eight bone metastases specimens from breast cancers were divided into three groups and subjected to different decalcifying agents (group A-10% nitrate, group B-EDTA decalcification, and group C-imported decalcifying solution RapidCal). The effects of those on HE and IHC staining for Ki-67, ER, PR, GATA3, RANK, RANKL, HER2 and HER2 FISH results were assessed. Results: There were no significant differences among three groups in HE morphology and IHC staining...
August 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/28806375/physics-and-histologic-evaluation-of-rotary-ultrasonic-and-sonic-instruments
#17
Emanuele Ruga, Ettore Amerio, Vincenzo Carbone, Marco Volante, Sergio Gandolfo
Rotary instruments (RIs) are the most commonly used to perform osteotomies in many fields of medicine. Owing to a new interest in performing a minimally invasive surgery, over last fifteen years new devices have been used in oral surgery such as ultrasonic instruments (UIs) and, lately, sonic instruments (SIs). Nowadays, bone preservation and regeneration are paramount in many clinical situations and, consequently, it is crucial to rely upon instruments, which cause the least tissue damage during the surgery...
October 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28797001/scintigraphic-patterns-of-indium-111-oxine-white-blood-cell-imaging-of-gram-negative-versus-gram-positive-vertebral-osteomyelitis
#18
Abdelhamid H Elgazzar, Shorouk Dannoon, Ismet Sarikaya, Medhat Farghali, Thamradeen A Junaid
OBJECTIVE: The goal of the study was to investigate whether or not Gram-negative organisms that secret anti-chemotactic factors caused the non-accumulation pattern of 111In-oxine-labeled white blood cell (111In WBC) scan. MATERIALS AND METHODS: Staphylococcus aureus (Gram-positive) (group 1) was injected into 25 rabbits and Escherichia coli (Gram-negative) (group 2) to another 25 to induce infection in the lumbar vertebrae or left thigh bone (femur). Sixteen rabbits of each group that were successfully infected surviving rabbits were used for imaging and analysis...
August 8, 2017: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
https://www.readbyqxmd.com/read/28731055/choroidal-excavation-in-choroidal-osteoma-complicated-by-choroidal-neovascularization
#19
L Pierro, A Marchese, M Gagliardi, U Introini, M Battaglia Parodi, G Casalino, F Bandello
PurposeTo describe multimodal imaging features of choroidal osteoma (CO) complicated by choroidal neovascularization (CNV) and focal choroidal excavation (FCE).MethodsPatients presenting with CO and CNV between January and October 2016 were considered for this study. Diagnosis of CO was confirmed by ultrasound examination. All patients underwent multimodal imaging including optical coherence tomography (OCT), swept-source OCT angiography (DRI OCT Triton, Topcon, Inc., Tokyo, Japan) and fluorescein angiography (Spectralis HRA+OCT; Heidelberg Engineering, Heidelberg, Germany)...
July 21, 2017: Eye
https://www.readbyqxmd.com/read/28726666/-pathomorphological-changes-under-latent-mastoiditis
#20
Kh Chitadze, T Jorbenadze, M Adamia
In our opinion it is caused by incomplete awareness of the distribution of pathological process and the character of morphological changes in the patients. In this regard, we studied structural changes in the soft and bone tissue taken from various cavities of the middle ear system. Operating material was formed in paraffin after the fixation and proper processing. We proceed with decalcification of bone tissues in 15-20% nitric acid. We painted 5-6 mm thick peels prepared on the rotating microtome with hematoxylin and eosin...
June 2017: Georgian Medical News
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