keyword
MENU ▼
Read by QxMD icon Read
search

Decalcification

keyword
https://www.readbyqxmd.com/read/28810293/-comparative-study-of-three-different-decalcifying-solutions-in-bone-metastasis-specimens-with-breast-cancer
#1
H Y Wu, T Wang, K L Chen, Z W Fan, X H Pu, B Zhang, X S Fan, F Q Meng
Objective: To investigate the optimal strategy for immunohistochemical (IHC) staining in bone metastasis specimens from breast cancer. Methods: Twenty-eight bone metastases specimens from breast cancers were divided into three groups and subjected to different decalcifying agents (group A-10% nitrate, group B-EDTA decalcification, and group C-imported decalcifying solution RapidCal). The effects of those on HE and IHC staining for Ki-67, ER, PR, GATA3, RANK, RANKL, HER2 and HER2 FISH results were assessed. Results: There were no significant differences among three groups in HE morphology and IHC staining...
August 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/28806375/physics-and-histologic-evaluation-of-rotary-ultrasonic-and-sonic-instruments
#2
Emanuele Ruga, Ettore Amerio, Vincenzo Carbone, Marco Volante, Sergio Gandolfo
Rotary instruments (RIs) are the most commonly used to perform osteotomies in many fields of medicine. Owing to a new interest in performing a minimally invasive surgery, over last fifteen years new devices have been used in oral surgery such as ultrasonic instruments (UIs) and, lately, sonic instruments (SIs). Nowadays, bone preservation and regeneration are paramount in many clinical situations and, consequently, it is crucial to rely upon instruments, which cause the least tissue damage during the surgery...
August 10, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28797001/scintigraphic-patterns-of-indium-111-oxine-white-blood-cell-imaging-of-gram-negative-versus-gram-positive-vertebral-osteomyelitis
#3
Abdelhamid H Elgazzar, Shorouk Dannoon, Ismet Sarikaya, Medhat Farghali, Thamradeen A Junaid
OBJECTIVE: The goal of the study was to investigate whether or not Gram-negative organisms that secret anti-chemotactic factors caused the non-accumulation pattern of 111In-oxine-labeled white blood cell (111In WBC) scan. MATERIALS AND METHODS: Staphylococcus aureus (Gram-positive) (group 1) was injected into 25 rabbits and Escherichia coli (Gram-negative) (group 2) to another 25 to induce infection in the lumbar vertebrae or left thigh bone (femur). Sixteen rabbits of each group that were successfully infected surviving rabbits were used for imaging and analysis...
August 8, 2017: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
https://www.readbyqxmd.com/read/28731055/choroidal-excavation-in-choroidal-osteoma-complicated-by-choroidal-neovascularization
#4
L Pierro, A Marchese, M Gagliardi, U Introini, M Battaglia Parodi, G Casalino, F Bandello
PurposeTo describe multimodal imaging features of choroidal osteoma (CO) complicated by choroidal neovascularization (CNV) and focal choroidal excavation (FCE).MethodsPatients presenting with CO and CNV between January and October 2016 were considered for this study. Diagnosis of CO was confirmed by ultrasound examination. All patients underwent multimodal imaging including optical coherence tomography (OCT), swept-source OCT angiography (DRI OCT Triton, Topcon, Inc., Tokyo, Japan) and fluorescein angiography (Spectralis HRA+OCT; Heidelberg Engineering, Heidelberg, Germany)...
July 21, 2017: Eye
https://www.readbyqxmd.com/read/28726666/-pathomorphological-changes-under-latent-mastoiditis
#5
Kh Chitadze, T Jorbenadze, M Adamia
In our opinion it is caused by incomplete awareness of the distribution of pathological process and the character of morphological changes in the patients. In this regard, we studied structural changes in the soft and bone tissue taken from various cavities of the middle ear system. Operating material was formed in paraffin after the fixation and proper processing. We proceed with decalcification of bone tissues in 15-20% nitric acid. We painted 5-6 mm thick peels prepared on the rotating microtome with hematoxylin and eosin...
June 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28688180/effect-of-silver-diamine-fluoride-sdf-on-the-dentin-pulp-complex-ex-vivo-histological-analysis-on-human-primary-teeth-and-rat-molars
#6
Glenda Rossi, Aldo Squassi, Patricia Mandalunis, Andrea Kaplan
The aim of this study was to determine the effect of SDF on the dentin-pulp complex using two models: teeth after SDF application (ex vivo) and experimental animal molars. A descriptive study was performed using two models. In the first model, primary teeth (ex vivo) with enamel-dentin caries, without pulp involvement and previously treated with 38% SDF, were evaluated by means of two techniques: (a) Scanning Electron Microscopy (SEM) and energy-dispersive X-ray detector (EDS) to determine qualitative and quantitative composition, and (b) brightfield optical microscopy (OM) after decalcification...
April 2017: Acta Odontológica Latinoamericana: AOL
https://www.readbyqxmd.com/read/28672969/comparative-study-on-the-biomechanics-between-improved-pvp-and-traditional-pkp-in-the-treatment-of-vertebral-peripheral-wall-damage-type-ovcf
#7
Tao Zhou, Hao Lin, Hongliang Wang, Xiaoqiang Chen, Fang He
We compared the biomechanics between improved percutaneous vertebroplasty (improved PVP) and traditional percutaneous kyphoplasty (PKP) in the treatment of vertebral peripheral wall damage-type osteoporotic vertebral compression fracture (OVCF). A total of 15 vertebral peripheral wall damage-type OVCF models of new calves (12-14 weeks) were treated with a decalcifying agent (Shandon TBD-1) with the vertebral compression fracture. The vertebral volume and anterior height before modeling, and the vertebral BMD before and after modeling were measured...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28604492/a-microstructural-investigation-of-disc-disruption-induced-by-low-frequency-cyclic-loading
#8
Meredith L Schollum, Kelly R Wade, Peter A Robertson, Ashvin Thambyah, Neil D Broom
STUDY DESIGN: Microstructural investigation of low frequency cyclic loading and flexing of the lumbar disc. OBJECTIVE: To explore micro-level structural damage in motion segments subjected to low frequency repetitive loading and flexing at sub-acute loads. SUMMARY OF BACKGROUND DATA: Cumulative exposure to mechanical load has been implicated in low back pain and injury. The mechanical pathways by which cyclic loading physically affects spine tissues remain unclear, in part due to the absence of high quality microstructural evidence...
June 9, 2017: Spine
https://www.readbyqxmd.com/read/28588314/frequency-of-gnas-r201h-substitution-mutation-in-polyostotic-fibrous-dysplasia-pyrosequencing-analysis-in-tissue-samples-with-or-without-decalcification
#9
Su-Jin Shin, Seok Joo Lee, Sang Kyum Kim
Guanine nucleotide-binding protein/α-subunit (GNAS) mutations are involved in fibrous dysplasia (FD) pathogenesis. Here, we analyzed GNAS mutations in FD which were performed by pyrosequencing DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue. The mutation detection rate was determined in FD specimens with and without decalcification. GNAS mutation was identified in 28 cases out of 87 FDs (32.18%) [p.R201C (N = 14) and p.R201H (N = 14)]. GNAS mutation was more likely to occur in polyostotic FD (7/28, 25...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28580541/decalcification-prevention-around-orthodontic-brackets-bonded-to-bleached-enamel-using-different-topical-agents
#10
Ferial Ahmed Msallam, Mohammed El-Awady Grawish, Ahmad Mohammed Hafez, Yasser Lotfy Abdelnaby
BACKGROUND: The present study was conducted to evaluate the effect of different topical agents utilized for prevention of enamel decalcification around orthodontic brackets bonded to bleached and non-bleached enamel. METHODS: Human maxillary premolars (n = 120) were divided into two equal groups. Teeth in group I were left without bleaching while those in group II were bleached with Vivastyle gel. Metal brackets were bonded to all the teeth using light-cured adhesive...
December 2017: Progress in Orthodontics
https://www.readbyqxmd.com/read/28580397/imaging-and-mapping-of-mouse-bone-using-maldi-imaging-mass-spectrometry
#11
Yoko Fujino, Tomoko Minamizaki, Hirotaka Yoshioka, Mitsugi Okada, Yuji Yoshiko
Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is an advanced method used globally to analyze the distribution of biomolecules on tissue cryosections without any probes. In bones, however, hydroxyapatite crystals make it difficult to determine the distribution of biomolecules using MALDI-IMS. Additionally, there is limited information regarding the use of this method to analyze bone tissues. To determine whether MALDI-IMS analysis of bone tissues can facilitate comprehensive mapping of biomolecules in mouse bone, we first dissected femurs and tibiae from 8-week-old male mice and characterized the quality of multiple fixation and decalcification methods for preparation of the samples...
December 2016: Bone Reports
https://www.readbyqxmd.com/read/28566845/prevention-and-treatment-of-white-spot-lesions-in-orthodontic-patients
#12
REVIEW
Maryam Khoroushi, Marzie Kachuie
Decalcification of enamel, appearing as white spot lesions (WSLs), around fixed orthodontic appliances is a major challenge during and after fixed orthodontic treatment by considering the fact that the goal of orthodontic treatment is to enhance facial and dental esthetic appearance. Banded or bonded teeth exhibit a significantly higher rate of WSLs compared to the controls with no braces as fixed appliances and the bonding materials promote retention of biofilms. These lesions are managed in the first step by establishing good oral hygiene habits and prophylaxis with topical fluorides, including high-fluoride toothpastes, fluoride mouthwashes, gels, varnishes, fluoride-containing bonding materials, and elastic ligatures...
January 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/28549358/-complication-analysis-in-lisfranc-fracture-dislocation
#13
R J García-Renedo, A Carranza-Bencano, R Leal-Gómez, F Cámara-Arrigunaga
OBJECTIVE: To value the complications and sequels for patients with Lisfrancs fracture-luxation (FLL). MATERIAL AND METHODS: A transverse ambispective study of cohorts was realized of 83 patients by diagnosis of Lisfrancs fracture-luxation. There was in use a protocol of withdrawal of information with variables related to the injury, clinical, therapeutic parameters, complications and scales of clinical valuation. RESULTS: 66 patients (79...
November 2016: Acta Ortopédica Mexicana
https://www.readbyqxmd.com/read/28527949/microproteome-of-dentoalveolar-tissues
#14
Cristiane R Salmon, Ana Paula O Giorgetti, Adriana F Paes Leme, Romênia R Domingues, Tamara N Kolli, Brian L Foster, Francisco H Nociti
Proteomic analysis of extracellular matrices (ECM) of dentoalveolar tissues can provide insights into developmental, pathological, and reparative processes. However, targeted dissection of mineralized tissues, dental cementum (DC), alveolar bone (AB), and dentin (DE), presents technical difficulties. We demonstrate an approach combining EDTA decalcification and laser capture microdissection (LCM), followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), to analyze proteome profiles of these tissues...
May 17, 2017: Bone
https://www.readbyqxmd.com/read/28502741/high-metabolic-activity-of-tissue-nonspecific-alkaline-phosphatase-not-only-in-young-but-also-in-adult-bone-as-demonstrated-using-a-new-histochemical-detection-protocol
#15
I Piotr Maly, Elisabeth Eppler, Magdalena Müller-Gerbl
Tissue-nonspecific alkaline phosphatase (TNAP) is playing a key role in bone calcification, as has been demonstrated in different mammalian species including human and rodents. However, to investigate age-related changes during life history, histochemical demonstration of TNAP is severely hampered, particularly in the elderly, by technical difficulties associated with sectioning calcified tissue. Sufficient fixation must precede decalcification since poorly fixed bone tissue is exposed to the deleterious effects of decalcification reagents...
May 11, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28501995/one-step-preservation-and-decalcification-of-bony-tissue-for-molecular-profiling
#16
Claudius Mueller, Michael G Harpole, Virginia Espina
Bone metastasis from primary cancer sites creates diagnostic and therapeutic challenges. Calcified bone is difficult to biopsy due to tissue hardness and patient discomfort, thus limiting the frequency and availability of bone/bone marrow biopsy material for molecular profiling. In addition, bony tissue must be demineralized (decalcified) prior to histomorphologic analysis. Decalcification processes rely on three main principles: (a) solubility of calcium salts in an acid, such as formic or nitric acid; (b) calcium chelation with ethylenediaminetetraacetic acid (EDTA); or (c) ion-exchange resins in a weak acid...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28501354/calcium-orthophosphates-capo4-occurrence-and-properties
#17
REVIEW
S V Dorozhkin
The present overview is intended to point the readers' attention to the important subject of calcium orthophosphates (CaPO4). This type of materials is of the special significance for the human beings because they represent the inorganic part of major normal (bones, teeth and antlers) and pathological (those appearing due to various diseases) calcified tissues of mammals. For example, atherosclerosis results in blood vessel blockage caused by a solid composite of cholesterol with CaPO4, while dental caries (tooth decay) and osteoporosis (a low bone mass with microarchitectural changes) mean a partial decalcification of teeth and bones, respectively, that results in replacement of a less soluble and harder biological apatite by more soluble and softer calcium hydrogenorthophosphates...
May 10, 2017: Morphologie: Bulletin de L'Association des Anatomistes
https://www.readbyqxmd.com/read/28424781/postembedding-decalcification-of-mineralized-tissue-sections-preserves-the-integrity-of-implanted-biomaterials-and-minimizes-number-of-experimental-animals
#18
Thaqif El Khassawna, Diaa Eldin S Daghma, Sabine Stoetzel, Seemun Ray, Stefanie Kern, Deeksha Malhan, Volker Alt, Ulrich Thormann, Anja Henß, Marcus Rohnke, Annette Stengel, Fathi Hassan, Stefan Maenz, Klaus D Jandt, Michael Diefenbeck, Matthias Schumacher, Michael Gelinsky, Katrin Susanne Lips, Christian Heiss
Bone histology of decalcified or undecalcified samples depends on the investigation. However, in research each method provides different information to answer the scientific question. Decalcification is the first step after sample fixation and governs what analysis is later feasible on the sections. Besides, decalcification is favored for immunostaining and in situ hybridization. Otherwise, sample decalcification can be damaging to bone biomaterials implants that contains calcium or strontium. On the other hand, after decalcification mineralization cannot be assessed using histology or imaging mass spectrometry...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28407126/mitral-valve-replacement-in-severely-calcified-mitral-valve-annulus-a-10-year-experience
#19
Kareem Salhiyyah, Hassan Kattach, Ahmed Ashoub, Diana Patrick, Szabolcs Miskolczi, Geoffrey Tsang, Sunil K Ohri, Clifford W Barlow, Theodore Velissaris, Steve Livesey
OBJECTIVES: Severe calcification in the mitral valve annulus is a challenging problem during mitral valve surgery. We describe our experience with mitral valve replacement in severely calcified mitral valve without decalcification of the annulus. METHODS: Between April 2001 and July 2011, 61 patients underwent mitral valve replacement with severe mitral annulus calcification without decalcification of the annulus. This retrospective study was performed to assess the surgical and the long-term postoperative outcomes in this group...
April 11, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28406968/the-sense-of-balance-in-humans-structural-features-of-otoconia-and-their-response-to-linear-acceleration
#20
Rüdiger Kniep, Dirk Zahn, Jana Wulfes, Leif Erik Walther
We explored the functional role of individual otoconia within the otolith system of mammalians responsible for the detection of linear accelerations and head tilts in relation to the gravity vector. Details of the inner structure and the shape of intact human and artificial otoconia were studied using environmental scanning electron microscopy (ESEM), including decalcification by ethylenediaminetetraacetic acid (EDTA) to discriminate local calcium carbonate density. Considerable differences between the rhombohedral faces of human and artificial otoconia already indicate that the inner architecture of otoconia is not consistent with the point group -3m...
2017: PloS One
keyword
keyword
22223
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"