keyword
MENU ▼
Read by QxMD icon Read
search

Decalcification

keyword
https://www.readbyqxmd.com/read/27891473/a-comparison-of-conventional-and-microwave-decalcification-and-processing-of-tooth-and-mandibular-bone-specimens
#1
A Thirumal Raj, Shankargouda Patil, Roopa S Rao
INTRODUCTION: Any laboratory procedure exposes the technician and the pathologists to the hazardous effects of chemicals. Conventional procedures like decalcification and histo-processing employed in laboratories are labour intense and time consuming thereby delaying the report dispatch. The present study was an attempt to employ a kitchen microwave to hasten the process and facilitate faster and accurate reporting; thus, benefitting the technician, pathologist and the patient. AIM: To compare conventional and microwave based decalci-fication, processing and staining of tooth and mandibular bone specimens using 5% nitric acid as decalcifying agent...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27863377/variable-patterns-of-ectopic-mineralization-in-enpp1asj-2j-mice-a-model-for-generalized-arterial-calcification-of-infancy
#2
Sarah Y Siu, Nathaniel A Dyment, David W Rowe, John P Sundberg, Jouni Uitto, Qiaoli Li
Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder characterized by early onset of extensive mineralization of the cardiovascular system. The classical forms of GACI are caused by mutations in the ENPP1 gene, encoding a membrane-bound pyrophosphatase/phosphodiesterase that hydrolyzes ATP to AMP and inorganic pyrophosphate. The asj-2J mouse harboring a spontaneous mutation in the Enpp1 gene has been characterized as a model for GACI. These mutant mice develop ectopic mineralization in skin and vascular connective tissues as well as in cartilage and collagen-rich tendons and ligaments...
November 14, 2016: Oncotarget
https://www.readbyqxmd.com/read/27855678/augmentation-and-repair-of-tendons-using-demineralised-cortical-bone
#3
Sherif Elnikety, Catherine J Pendegrass, Roberta Ferro de Godoy, Charles Holden, Gordon W Blunn
BACKGROUND: In severe injuries with loss of tendon substance a tendon graft or a synthetic substitute is usually used to restore functional length. This is usually associated with donor site morbidity, host tissue reactions and lack of remodelling of the synthetic substitutes, which may result in suboptimal outcome. A biocompatible graft with mechanical and structural properties that replicate those of normal tendon and ligament has so far not been identified. The use of demineralised bone for tendon reattachment onto bone has been shown to be effective in promoting the regeneration of a normal enthesis...
November 17, 2016: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/27849308/sutureless-aortic-prosthesis-implantation-the-first-brazilian-experience-with-perceval-device
#4
Ana Paula Tagliari, Leandro de Moura, Luiz Henrique Dussin, Eduardo Keller Saadi
This is a report of the first Brazilian experience with the Perceval sutureless aortic prosthesis in two patients with severe aortic stenosis. Transesophageal echocardiography was used during the procedure. The aortotomy was performed 1 cm above the sinotubular junction, followed by leaflets removal and decalcification. Correct valve size was selected, device released and an accommodation balloon used. The cardiopulmonary bypass times were 47 and 38 min and the cross-clamp times were 38 and 30 min. There was a significant decrease in mean gradients (41 and 75 mmHg preoperatively; 7 and 8 mmHg postoperatively)...
July 2016: Brazilian Journal of Cardiovascular Surgery
https://www.readbyqxmd.com/read/27744044/pushing-the-limits-in-transcatheter-aortic-valve-replacement-high-volume-center-s-effect-overconfidence-or%C3%A2-something%C3%A2-else
#5
Francesco Nappi, Cristiano Spadaccio, Jean Louis Sablayrolles
The recent literature on transcatheter aortic valve replacement (TAVR) is shedding new light on the perspective to extend this procedure to other lower risk-category of patients, leading in fact to a potential erosion of the current guidelines. Notwithstanding the warnings provided in the literature regarding the risk of severely impairing complications, unclear survival advantage and cost-inefficiency, many observational studies, especially performed in high-volume centers, support a general drive toward the recruitment of intermediate-low risk patients in the expectation of clinical advantages versus standard surgical replacement...
October 6, 2016: JACC. Cardiovascular Interventions
https://www.readbyqxmd.com/read/27734089/efficacy-of-a-new-sealant-to-prevent-white-spot-lesions-during-fixed-orthodontic-treatment-a-12-month-single-center-randomized-controlled-clinical-trial
#6
Shaza M Hammad, Michael Knösel
BACKGROUND AND OBJECTIVE: White spot lesions (WSLs) are an undesirable side effect of fixed orthodontic appliance therapy and are reported to occur in 2-96 % of orthodontic patients. In this study, the efficacy of a new sealant to prevent WSLs during fixed orthodontic treatment was compared to a control group that did not receive sealant. PATIENTS AND METHODS: For this 2-arm parallel-group randomized trial, 50 subjects aged 12-18 years (mean age 14.57 ± 2...
October 12, 2016: Journal of Orofacial Orthopedics, Fortschritte der Kieferorthopädie
https://www.readbyqxmd.com/read/27725521/irradiation-effects-of-low-temperature-multi-gas-plasma-jet-on-oral-bacteria
#7
Tahsin Raquib Abonti, Masato Kaku, Shunichi Kojima, Hiromi Sumi, Shotoku Kojima, Taeko Yamamoto, Yuka Yashima, Hidekazu Miyahara, Akitoshi Okino, Toshitsugu Kawata, Kazuo Tanne, Kotaro Tanimoto
The purpose of this study is to evaluate the sterilization effects of a newly developed low temperature multi gas plasma jet on oral pathogenic microorganisms (Streptococcus mutans [S. mutans], Lactobacillus fermentum [L. fermentum], Aggregatibacter actinomycetemcomitans [A. actinomycetemcomitans]). Plasma gas which generated from O2, N2, Ar and 50% (O2+N2) was irradiated to the microbes. Effect of O2 plasma irradiation on S. mutans under scanning electron microscopy (SEM) was also observed. O2 plasma was directly applied to dental plaque on human extracted tooth...
2016: Dental Materials Journal
https://www.readbyqxmd.com/read/27725518/effect-of-different-acidities-of-electrolyzed-water-on-dentin-surface-roughness-decalcification-and-microhardness-a-preliminary-study
#8
Ker-Kong Chen, Chau-Hsiang Wang, Chun-Chan Ting, Shin-I Wei, Je-Kang DU
This study investigated the exposed coronal superficial dentin of 28 human molars were immersed in strongly acidic electrolyzed water (AW group), neutral electrolyzed water (NW group), 5% sodium hypochlorite (negative control, NL group), or deionized water (positive control, DW group). Microhardness was determined at 5-min intervals for 60 min. Surface roughness, energy dispersive X-ray spectroscopy and scanning electron microscopy analyses were performed after 5 and 60 min. Reductions in microhardness were observed in the AW, NW and NL groups, with further decreases as immersion time increased...
2016: Dental Materials Journal
https://www.readbyqxmd.com/read/27622177/an-experimental-study-for-minimum-level-of-decalcification-to-detect-the-osteolytic-bone-metastasis-of-long-bone-on-plain-radiography
#9
Jun Ho Baek, Il Hyung Park, Sung Hwa Seo
BACKGROUND: In 1951, Ardran reported that metastatic bone lesions could be detectable on plain radiography with 30% to 50% of decalcification. Authors performed experimental study for minimum level of decalcification to detect the osteolytic bone metastasis of long bone with recent technique of radiographs. METHODS: One pair of fibula and humerus from two cadavers was cut into specimen 1 inch in length. Distal half of specimen was dipped into hydrochloride (HCl) with 15 min interval...
August 2016: Journal of Bone Metabolism
https://www.readbyqxmd.com/read/27610135/endobronchial-carcinoid-tumour-with-extensive-ossification-an-unusual-case-presentation
#10
Allison Osmond, Emily Filter, Mariamma Joseph, Richard Inculet, Keith Kwan, David McCormack
Carcinoid tumour is a well-known primary endobronchial lung neoplasm. Although calcifications may be seen in up to 30% of pulmonary carcinoid tumours, near complete ossification of these tumours is an unusual finding. Such lesions can prove diagnostically challenging at the time of intraoperative frozen section as the latter technique requires thin sectioning of the lesion for microscopic assessment. We present an unusual case of endobronchial carcinoid tumour with extensive ossification in a 45-year-old male...
2016: Case Reports in Medicine
https://www.readbyqxmd.com/read/27603892/-gastrointestinal-and-hepatic-diseases
#11
Carlos Moctezuma-Velázquez, Jonathan Aguirre-Valadez
Diet is considered an important triggering factor for gastrointestinal symptoms whose physiopathology includes not only measurable, inflammatory reactions, but also functional disorders, where no organic effects may be measured or demonstrated. Moreover, the prevalence of the perceived intolerance to certain foods ranges from 20-25% (within the general population) to 50-70% in diseases like irritable bowel syndrome. This intolerance has been observed particularly after the consumption of milk and dairy products, which are frequently considered as causative of gastrointestinal symptoms, thus limiting their ingestion...
September 2016: Gaceta Médica de México
https://www.readbyqxmd.com/read/27572616/incidence-and-predictors-of-new-onset-atrioventricular-block-requiring-pacemaker-implantation-after-sutureless-aortic-valve-replacement
#12
Beatriz Toledano, Felipe Bisbal, Maria Luisa Camara, Carlos Labata, Elisabet Berastegui, Carolina Gálvez-Montón, Roger Villuendas, Axel Sarrias, Teresa Oliveres, Damià Pereferrer, Xavier Ruyra, Antoni Bayés-Genís
OBJECTIVES: In high-risk patients with severe aortic stenosis, aortic valve replacement (AVR) with a sutureless Perceval prosthesis (SU-AVR) can be performed instead of conventional AVR or transcatheter aortic valve implantation. Little data are available regarding postoperative conduction disorders after SU-AVR. We aimed to determine the incidence and predictors of new-onset complete atrioventricular block (NO-AVB) requiring permanent cardiac stimulation following SU-AVR. METHODS: We studied consecutive patients who underwent SU-AVR between 2013 and 2015...
August 29, 2016: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/27562496/influence-of-decalcification-procedures-on-immunohistochemistry-and-molecular-pathology-in-breast-cancer
#13
Willemijne A M E Schrijver, Petra van der Groep, Laurien Dc Hoefnagel, Natalie D Ter Hoeve, Ton Peeters, Cathy B Moelans, Paul J van Diest
Distant breast cancer metastases are nowadays routinely biopsied to reassess receptor status and to isolate DNA for sequencing of druggable targets. Bone metastases are the most frequent subgroup. Decalcification procedures may negatively affect antigenicity and DNA quality. We therefore evaluated the effect of several decalcification procedures on receptor status and DNA/RNA quality. In 23 prospectively collected breast tumors, we compared ERα, PR and HER2 status by immunohistochemistry in (non-decalcified) tissue routinely processed for diagnostic purposes and in parallel tissue decalcified in Christensen's buffer with and without microwave, EDTA and Formical-4...
August 26, 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/27538158/-preliminary-research-of-er-yag-laser-used-for-pulpotomy-of-beagle-dogs
#14
Y D Guo, S Zhang
OBJECTIVE: To investigate the effects of erbium:yttrium aluminium garnet (Er:YAG)laser irradiation on pulp and formation of dentin bridge. The beagle dogs' coronal pulp is amputated by Er:YAG laser. METHODS: In the study, 24 premolar teeth of two one-year-old Beagle dogs were used. They were divided into 3 groups: bur group, 200 mJ laser group,100 mJ laser group. The coronal pulp were removed by traditional bur or 200 mJ/20 Hz or 100 mJ/20 Hz Er:YAG laser. Then, they were dressed with mineral trioxide aggregate (MTA) and sealed with resin...
February 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/27523626/influence-of-nonenzymatic-glycation-in-dentinal-collagen-on-dental-caries
#15
Y Matsuda, J Miura, M Shimizu, T Aoki, M Kubo, S Fukushima, M Hashimoto, F Takeshige, T Araki
Advanced glycation end-products (AGEs) are generated via nonenzymatic glycation of dentinal collagen, resulting in accumulation of AGEs in dentin tissue. Since accumulated AGEs cause crosslinking between amino acid polypeptides in the collagen molecule and modify mechanical properties of dentinal collagen, the authors assumed that there would be a significant interaction between the generation of AGEs and progression of caries in dentin. To confirm such an interaction, spectroscopic imaging analyses (i.e., nanosecond fluorescence lifetime imaging and second harmonic generation light imaging) were performed in addition to biochemical and electron microscopic analyses in the present study...
August 14, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27512386/changes-in-skeletal-integrity-and-marrow-adiposity-during-high-fat-diet-and-after-weight-loss
#16
Erica L Scheller, Basma Khoury, Kayla L Moller, Natalie K Y Wee, Shaima Khandaker, Kenneth M Kozloff, Simin H Abrishami, Brian F Zamarron, Kanakadurga Singer
The prevalence of obesity has continued to rise over the past three decades leading to significant increases in obesity-related medical care costs from metabolic and non-metabolic sequelae. It is now clear that expansion of body fat leads to an increase in inflammation with systemic effects on metabolism. In mouse models of diet-induced obesity, there is also an expansion of bone marrow adipocytes. However, the persistence of these changes after weight loss has not been well described. The objective of this study was to investigate the impact of high-fat diet (HFD) and subsequent weight loss on skeletal parameters in C57Bl6/J mice...
2016: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/27500784/effects-of-qmix-and-ethylenediaminetetraacetic-acid-on-decalcification-and-erosion-of-root-canal-dentin
#17
Hacer Aksel, Ahmet Serper, Sukru Kalayci, Guler Somer, Cevat Erisken
The aim of this study was to evaluate the effect of initial NaOCl on the decalcification and erosion ability of EDTA and QMix. Sixty-maxillary-incisors were bisected longitudinally and the tooth-halves were used. The experiment was conducted in two-sets. In set-I, 80-tooth halves were treated in the presence or absence of initial NaOCl and EDTA. In set-II, 40-tooth halves were immersed in NaOCl and QMix. After each treatment, calcium-ion release was determined with flame photometry. The erosion was imaged using SEM...
August 8, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27499316/is-there-crosstalk-between-subchondral-bone-cartilage-and-meniscus-in-the-pathogenesis-of-osteoarthritis
#18
O Şahap Atik, Deniz Erdoğan, Cemile Merve Seymen, Hasan Hüseyin Bozkurt, Gülnur Take Kaplanoğlu
OBJECTIVES: This study aims to investigate if there is any crosstalk between subchondral bone, cartilage, and meniscus in the pathogenesis of osteoarthritis. PATIENTS AND METHODS: Twelve female patients (mean age 64 years; range 59 to 71 years) with osteoarthritis in medial compartment were included in the study. The samples of subchondral bone, cartilage and meniscus were obtained during total knee arthroplasty. Degenerated tissue samples obtained from medial compartment were used as the experimental group (12 samples of subchondral bone and cartilage, 1x1 cm each; and 12 samples of meniscus, 1x1 cm each)...
August 2016: Eklem Hastalıkları Ve Cerrahisi, Joint Diseases & related Surgery
https://www.readbyqxmd.com/read/27478409/the-study-of-calcified-atherosclerotic-arteries-an-alternative-to-evaluate-the-composition-of-a-problematic-tissue-reveals-new-insight%C3%A2-including-metakaryotic-cells
#19
Silvia Fittipaldi, Francesco Vasuri, Alessio Degiovanni, Rodolfo Pini, Mauro Gargiulo, Andrea Stella, Gianandrea Pasquinelli, William G Thilly, Elena V Gostjeva
BACKGROUND: Calcifications of atherosclerotic plaques represent a controversial issue as they either lead to the stabilization or rupture of the lesion. However, the cellular key players involved in the progression of the calcified plaques have not yet been described. The primary reason for this lacuna is that decalcification procedures impair protein and nucleic acids contained in the calcified tissue. The aim of our study was to preserve the cellular content of heavily calcified plaques with a new rapid fixation in order to simplify the study of calcifications...
2016: BMC Clinical Pathology
https://www.readbyqxmd.com/read/27471662/calcium-orthophosphates-capo4-occurrence-and-properties
#20
Sergey V Dorozhkin
The present overview is intended to point the readers' attention to the important subject of calcium orthophosphates (CaPO4). This type of materials is of the special significance for the human beings because they represent the inorganic part of major normal (bones, teeth and antlers) and pathological (i.e., those appearing due to various diseases) calcified tissues of mammals. For example, atherosclerosis results in blood vessel blockage caused by a solid composite of cholesterol with CaPO4, while dental caries and osteoporosis mean a partial decalcification of teeth and bones, respectively, that results in replacement of a less soluble and harder biological apatite by more soluble and softer calcium hydrogenorthophosphates...
2016: Progress in biomaterials
keyword
keyword
22223
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"