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Decalcification

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https://www.readbyqxmd.com/read/28297096/accelerated-orthodontic-treatment-what-s-the-evidence
#1
P Miles
The demand and accessibility of orthodontic care has increased but has also been accompanied by patient requests for shorter treatment times. Longer orthodontic treatment increases the risk of decalcification, gingival recession, and root resorption and so shorter treatment times have multiple advantages as well as appealing to patient's desires. Numerous techniques and materials have been suggested to reduce treatment times but, in most cases, are based upon selected case reports with no prospective clinical trials to validate claims...
March 2017: Australian Dental Journal
https://www.readbyqxmd.com/read/28259046/organic-extraction-of-bone-lysates-improves-dna-purification-with-silica-beads
#2
Stijn Desmyter, Greet De Cock, Sabine Moulin, Fabrice Noël
In our standard protocol for DNA extraction from skeletal remains of unidentified bodies, bone lysates resulting from decalcification and Proteinase K treatment were purified with the DNA IQ™ Casework Pro Kit for Maxwell(®)16 automate (Promega, WI). Despite its success in the majority of cases, the DNA purification with paramagnetic silica beads failed in some challenging samples. This failure in DNA recovery was often associated with filter clogging during the required volume reduction of the lysate to enable loading on the automate...
February 15, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28246608/evaluation-of-decalcification-techniques-for-rat-femurs-using-he-and-immunohistochemical-staining
#3
Haixia Liu, Ruyuan Zhu, Chenyue Liu, Rufeng Ma, Lili Wang, Beibei Chen, Lin Li, Jianzhao Niu, Dandan Zhao, Fangfang Mo, Min Fu, Dieter Brömme, Dongwei Zhang, Sihua Gao
Aim. In routine histopathology, decalcification is an essential step for mineralized tissues. The purpose of this study is to evaluate the effects of different decalcification solutions on the morphological and antigenicity preservation in Sprague Dawley (SD) rat femurs. Materials and Methods. Four different decalcification solutions were employed to remove the mineral substances from rat femurs, including 10% neutral buffered EDTA, 3% nitric acid, 5% nitric acid, and 8% hydrochloric acid/formic acid. Shaking and low temperature were used to process the samples...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28230910/adhesives-for-bonded-molar-tubes-during-fixed-brace-treatment
#4
REVIEW
Declan T Millett, Nicky A Mandall, Rye Cr Mattick, Joy Hickman, Anne-Marie Glenny
BACKGROUND: Orthodontic treatment involves using fixed or removable appliances (dental braces) to correct the positions of teeth. The success of a fixed appliance depends partly on the metal attachments (brackets and bands) being glued to the teeth so that they do not become detached during treatment. Brackets (metal squares) are usually attached to teeth other than molars, where bands (metal rings that go round each tooth) are more commonly used. Orthodontic tubes (stainless steel tubes that allow wires to pass through them), are typically welded to bands but they may also be glued directly (bonded) to molars...
February 23, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28229056/experimental-visualization-of-labyrinthine-structure-with-optical-coherence-tomography
#5
Saleh Mohebbi, Marjan Mirsalehi, Lüder-Alexander Kahrs, Tobias Ortmaier, Thomas Lenarz, Omid Majdani
INTRODUCTION: Visualization of inner ear structures is a valuable strategy for researchers and clinicians working on hearing pathologies. Optical coherence tomography (OCT) is a high-resolution imaging technology which may be used for the visualization of tissues. In this experimental study we aimed to evaluate inner ear anatomy in well-prepared human labyrinthine bones. MATERIALS AND METHODS: Three fresh human explanted temporal bones were trimmed, chemically decalcified with ethylenediaminetetraacetic acid (EDTA), and mechanically drilled under visual control using OCT in order to reveal the remaining bone shell...
January 2017: Iranian Journal of Otorhinolaryngology
https://www.readbyqxmd.com/read/28208997/comparison-of-self-etch-primers-with-conventional-acid-etching-system-on-orthodontic-brackets
#6
Amit Zope, Yogita Zope-Khalekar, Shrikant S Chitko, Veerendra V Kerudi, Harshal Ashok Patil, Prasad Vasudeo Bonde, Pratik Jaltare, Siddhesh G Dolas
INTRODUCTION: The self-etching primer system consists of etchant and primer dispersed in a single unit. The etching and priming are merged as a single step leading to fewer stages in bonding procedure and reduction in the number of steps that also reduces the chance of introduction of error, resulting in saving time for the clinician. It also results in smaller extent of enamel decalcification. AIM: To compare the Shear Bond Strength (SBS) of orthodontic bracket bonded with Self-Etch Primers (SEP) and conventional acid etching system and to study the surface appearance of teeth after debonding; etching with conventional acid etch and self-etch priming, using stereomicroscope...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28196606/imaging-and-cell-count-in-cleared-intact-cochlea-in-the-mongolian-gerbil-using-laser-scanning-confocal-microscopy
#7
M Risoud, J Sircoglou, G Dedieu, M Tardivel, C Vincent, N-X Bonne
OBJECTIVES: To draw up a clearing protocol for Mongolian gerbil cochlea, and to assess the feasibility of quantifying and analyzing 3D cell architecture in the transparent cochleae. MATERIALS AND METHODS: Freshly dissected inner ears were prepared on a 13-day protocol: fixation, microdissection, post-fixation, decalcification, pretreatment (signal enhancement, permeabilization and blocking), fluorescent labeling (indirect immunolabeling and direct labeling), dehydration, clearing in Spalteholz solution (MSBB: methyl salicylate and benzyl benzoate) and mounting...
February 9, 2017: European Annals of Otorhinolaryngology, Head and Neck Diseases
https://www.readbyqxmd.com/read/28150745/first-real-time-observation-of-transverse-division-in-azooxanthellate-scleractinian-corals
#8
Yuki Tokuda, Hiroko Haraguchi, Yoichi Ezaki
Asexual reproduction is one of the most important traits in the evolutionary history of corals. No real-time observations of asexual reproduction in azooxanthellate solitary scleractinian corals have been conducted to date. Here, we describe previously unknown aspects of asexual reproduction by using Truncatoflabellum spheniscus (Family Flabellidae) based on observations of transverse division conducted over 1200 days. The findings revealed that (1) transverse division was caused by decalcification; (2) compared to the anthocyathus (upper part of the divided corallum), the soft parts of the anthocaulus (lower part of the divided corallum) were severely damaged and injured during division; (3) these injuries were repaired rapidly; and (4) the anthocaulus regrew and repeatedly produced anthocyathi by means of transverse division...
February 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28118552/decellularized-tooth-bud-scaffolds-for-tooth-regeneration
#9
W Zhang, B Vazquez, D Oreadi, P C Yelick
Whole tooth regeneration approaches currently are limited by our inability to bioengineer full-sized, living replacement teeth. Recently, decellularized organ scaffolds have shown promise for applications in regenerative medicine by providing a natural extracellular matrix environment that promotes cell attachment and tissue-specific differentiation leading to full-sized organ regeneration. We hypothesize that decellularized tooth buds (dTBs) created from unerupted porcine tooth buds (TBs) can be used to guide reseeded dental cell differentiation to form whole bioengineered teeth, thereby providing a potential off-the-shelf scaffold for whole tooth regeneration...
January 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28116985/rotary-ultrasonic-drilling-on-bone-a-novel-technique-to-put-an-end-to-thermal-injury-to-bone
#10
Vishal Gupta, Pulak M Pandey, Ravi K Gupta, Asit R Mridha
Bone drilling is common in orthopedic procedures and the heat produced during conventional experimental drilling often exceeds critical temperature of 47 °C and induces thermal osteonecrosis. The osteonecrosis may be the reason for impaired healing, early loosening and implant failure. This study was undertaken to control the temperature rise by interrupted cutting and reduced friction effects at the interface of drill tool and the bone surface. In this work, rotary ultrasonic drilling technique with diamond abrasive particles coated on the hollow drill tool without any internal or external cooling assistance was used...
March 2017: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/28098734/multimodal-fundus-imaging-of-outer-retinal-tubulations-in-choroidal-osteoma-patients
#11
Yi Xuan, Yongjin Zhang, Min Wang, Jinli Guo, Lei Li, Wei Liu, Xiaofeng Ye
PURPOSE: To evaluate the morphological findings of outer retinal tubulations (ORTs) shown on multimodal imaging modalities in patients with choroidal osteoma. METHODS: Nineteen eyes of 17 patients with choroidal osteoma underwent full clinical and imaging assessments. Color fundus photography, spectral-domain optical coherence tomography (OCT), and en face OCT were used to identify and detect the characteristics of ORT structures, including the shape, configuration, location, and distribution in the fundus...
January 16, 2017: Retina
https://www.readbyqxmd.com/read/28092288/sutureless-aortic-valves-who-is-the-right-patient
#12
Amine Mazine, Christopher Bonneau, Dimos Karangelis, Bobby Yanagawa, Subodh Verma, Daniel Bonneau
PURPOSE OF REVIEW: Sutureless aortic valve replacement (AVR) has emerged as an alternative to traditional AVR for patients with aortic stenosis who present a higher surgical risk, such as the elderly, or those with small or highly calcified aortic roots. With transcatheter aortic valve implantation - the other major AVR alternative - being used in increasingly lower-risk patients, the place of sutureless valves in the AVR landscape needs to be defined. In this review, we discuss recent data and expert opinion as it pertains to the subject of sutureless AVR...
March 2017: Current Opinion in Cardiology
https://www.readbyqxmd.com/read/28051148/tissue-microarray-analysis-applied-to-bone-diagenesis
#13
Rafael Barrios Mello, Maria Regina Regis Silva, Maria Teresa Seixas Alves, Martin Paul Evison, Marco Aurelio Guimarães, Rafaella Arrabaca Francisco, Rafael Dias Astolphi, Edna Sadayo Miazato Iwamura
Taphonomic processes affecting bone post mortem are important in forensic, archaeological and palaeontological investigations. In this study, the application of tissue microarray (TMA) analysis to a sample of femoral bone specimens from 20 exhumed individuals of known period of burial and age at death is described. TMA allows multiplexing of subsamples, permitting standardized comparative analysis of adjacent sections in 3-D and of representative cross-sections of a large number of specimens. Standard hematoxylin and eosin, periodic acid-Schiff and silver methenamine, and picrosirius red staining, and CD31 and CD34 immunohistochemistry were applied to TMA sections...
January 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28009953/mitral-valve-re-repair-in-an-adult-patient-having-undergone-mitral-ring-annuloplasty-at-five-years-of-age
#14
Jong Hun Kim, Tae Youn Kim, Jong Bum Choi, Ja Hong Kuh
In adult patients who have undergone mitral annuloplasty with a rigid ring at a young age, the mitral valve may ultimately deform and demonstrate insufficient growth because the valve annulus is fixed to the ring. Mitral valve re-repair, following this scenario, was performed for mitral stenoinsufficiency in a 26-year-old female patient who had undergone mitral annuloplasty with a rigid ring at the age of five years. The valve re-repair procedure consisted of decalcification and stripping of the anterior leaflet and annulus, posterior leaflet augmentation using an elliptical autologous pericardial patch, and a posterior strip annuloplasty sparing the anterior annulus and commissures...
July 2016: Journal of Heart Valve Disease
https://www.readbyqxmd.com/read/27965484/influence-of-dentin-deproteinization-on-bonding-degradation-1-year-results
#15
César R Pucci, Natália R Barbosa, Eduardo Bresciani, Karen Ck Yui, Maria Filomena Rl Huhtala, Daphne C Barcellos, Carlos Rg Torres
INTRODUCTION: The decalcification protocol of dentin surface with sodium hypochlorite removes the exposed collagen fibrils and could improve the longevity of the bonding interface. This study evaluated the influence of collagen removal with 10% sodium hypochlorite (10% NaOCl) on the longitudinal shear bond strength (SBS) of adhesives to dentin. MATERIALS AND METHODS: Seventy-two extracted human molars were sectioned and the buccal and lingual surfaces were flattened and acid etched with 37% phosphoric acid for 15 seconds...
December 1, 2016: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/27891473/a-comparison-of-conventional-and-microwave-decalcification-and-processing-of-tooth-and-mandibular-bone-specimens
#16
A Thirumal Raj, Shankargouda Patil, Roopa S Rao
INTRODUCTION: Any laboratory procedure exposes the technician and the pathologists to the hazardous effects of chemicals. Conventional procedures like decalcification and histo-processing employed in laboratories are labour intense and time consuming thereby delaying the report dispatch. The present study was an attempt to employ a kitchen microwave to hasten the process and facilitate faster and accurate reporting; thus, benefitting the technician, pathologist and the patient. AIM: To compare conventional and microwave based decalci-fication, processing and staining of tooth and mandibular bone specimens using 5% nitric acid as decalcifying agent...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27863377/variable-patterns-of-ectopic-mineralization-in-enpp1asj-2j-mice-a-model-for-generalized-arterial-calcification-of-infancy
#17
Sarah Y Siu, Nathaniel A Dyment, David W Rowe, John P Sundberg, Jouni Uitto, Qiaoli Li
Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder characterized by early onset of extensive mineralization of the cardiovascular system. The classical forms of GACI are caused by mutations in the ENPP1 gene, encoding a membrane-bound pyrophosphatase/phosphodiesterase that hydrolyzes ATP to AMP and inorganic pyrophosphate. The asj-2J mouse harboring a spontaneous mutation in the Enpp1 gene has been characterized as a model for GACI. These mutant mice develop ectopic mineralization in skin and vascular connective tissues as well as in cartilage and collagen-rich tendons and ligaments...
December 20, 2016: Oncotarget
https://www.readbyqxmd.com/read/27855678/augmentation-and-repair-of-tendons-using-demineralised-cortical-bone
#18
Sherif Elnikety, Catherine J Pendegrass, Roberta Ferro de Godoy, Charles Holden, Gordon W Blunn
BACKGROUND: In severe injuries with loss of tendon substance a tendon graft or a synthetic substitute is usually used to restore functional length. This is usually associated with donor site morbidity, host tissue reactions and lack of remodelling of the synthetic substitutes, which may result in suboptimal outcome. A biocompatible graft with mechanical and structural properties that replicate those of normal tendon and ligament has so far not been identified. The use of demineralised bone for tendon reattachment onto bone has been shown to be effective in promoting the regeneration of a normal enthesis...
November 17, 2016: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/27849308/sutureless-aortic-prosthesis-implantation-the-first-brazilian-experience-with-perceval-device
#19
Ana Paula Tagliari, Leandro de Moura, Luiz Henrique Dussin, Eduardo Keller Saadi
This is a report of the first Brazilian experience with the Perceval sutureless aortic prosthesis in two patients with severe aortic stenosis. Transesophageal echocardiography was used during the procedure. The aortotomy was performed 1 cm above the sinotubular junction, followed by leaflets removal and decalcification. Correct valve size was selected, device released and an accommodation balloon used. The cardiopulmonary bypass times were 47 and 38 min and the cross-clamp times were 38 and 30 min. There was a significant decrease in mean gradients (41 and 75 mmHg preoperatively; 7 and 8 mmHg postoperatively)...
July 2016: Brazilian Journal of Cardiovascular Surgery
https://www.readbyqxmd.com/read/27744044/pushing-the-limits-in-transcatheter-aortic-valve-replacement-high-volume-center-s-effect-overconfidence-or%C3%A2-something%C3%A2-else
#20
Francesco Nappi, Cristiano Spadaccio, Jean Louis Sablayrolles
The recent literature on transcatheter aortic valve replacement (TAVR) is shedding new light on the perspective to extend this procedure to other lower risk-category of patients, leading in fact to a potential erosion of the current guidelines. Notwithstanding the warnings provided in the literature regarding the risk of severely impairing complications, unclear survival advantage and cost-inefficiency, many observational studies, especially performed in high-volume centers, support a general drive toward the recruitment of intermediate-low risk patients in the expectation of clinical advantages versus standard surgical replacement...
October 6, 2016: JACC. Cardiovascular Interventions
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