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Decalcification

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https://www.readbyqxmd.com/read/29313383/comparison-of-decalcifying-agents-and-techniques-for-human-dental-tissues
#1
A Choube, M Astekar, A Choube, G Sapra, A Agarwal, A Rana
Teeth are among the hardest animal tissues, because they are composed of large amounts of inorganic compounds. Consequently, teeth are difficult to prepare for microscopic examination. Acids and chelating agents traditionally have been used to remove calcium ions. We compared decalcifying agents including strong acids, weak acids, chelating agents, techniques using electric current, agitation and heat. Freshly extracted teeth were fixed and decalcified using formic acid-formalin, formal-nitric acid, formalin-EDTA, Von Ebner's fluid and Perenyi's fluid...
January 9, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29299732/mi-varnish-and-mi-paste-plus-in-a-caries-prevention-and-remineralization-study-a-randomized-controlled-trial
#2
Peter Rechmann, Sona Bekmezian, Beate M T Rechmann, Benjamin W Chaffee, John D B Featherstone
OBJECTIVES: White spot lesions (WSLs) are a complication of orthodontic therapy. This study investigated the effect of MI (minimally invasive) Paste Plus (MIPP) and MI Varnish (MIV) on WSLs in orthodontic patients during a 12-month, randomized, single-blind, prospective, standard-of-care controlled clinical trial. MATERIALS AND METHODS: Forty subjects, recruited from the UCSF School of Dentistry Orthodontics Clinic, were randomly assigned to the experimental (twice-daily 1100 ppm fluoride toothpaste, daily MIPP, quarterly MIV application) or control group (twice-daily 1100 ppm fluoride toothpaste, fluoride rinse recommendation)...
January 4, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29260499/choroidal-osteoma-and-pattern-dystrophy-of-retinal-pigment-epithelium
#3
Vinod Kumar
PURPOSE: To describe co-occurrence of choroidal osteoma in a patient with pattern dystrophy of retinal pigment epithelium. METHODS: Clinical case report RESULTS: A young female presented with decreased vision in the right eye due to decalcification of choroidal osteoma. Multimodal imaging including fundus autofluorescence, fluorescein angiography and optical coherence tomography showed features of pattern dystrophy of retinal pigment epithelium that simulates the Stargardt disease...
December 19, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/29168058/analysis-of-siamese-crocodile-crocodylus-siamensis-eggshell-proteome
#4
Ivan Mikšík, Statis Paradis, Adam Eckhardt, David Sedmera
The proteins and pigment of the eggshell of the Siamese crocodile (Crocodylus siamensis) were analysed. For proteomic analysis, various decalcification methods were used when the two main surface layers were analyzed. These layers are important for antimicrobial defense of egg (particularly the cuticle). We found 58 proteins in both layers, of which 4 were specific for the cuticle and 26 for the palisade (honeycomb) layer. Substantial differences between proteins in the eggshell of crocodile and previously described birds' eggshells exist (both in terms of quality and quantity), however, the entire proteome of Crocodilians has not been described yet...
November 22, 2017: Protein Journal
https://www.readbyqxmd.com/read/29157956/comparative-analysis-of-fixation-and-embedding-techniques-for-optimized-histological-preparation-of-zebrafish
#5
Jean E Copper, Lynn R Budgeon, Christina A Foutz, Damian B van Rossum, Daniel J Vanselow, Margaret J Hubley, Darin P Clark, David T Mandrell, Keith C Cheng
In recognition of the importance of zebrafish as a model organism for studying human disease, we have created a web-based reference atlas of zebrafish microanatomy for comparing histology and histopathology between model systems and with humans (http://bio-atlas.psu.edu). Fixation, decalcification, embedding, and sectioning were optimized to maximize section quality. A comparison of protocols involving six fixatives showed that 10% Neutral Buffered Formalin at 21°C for 24h yielded excellent results. Sectioning of juveniles and adults requires bone decalcification; EDTA at 0...
November 17, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29131649/characterization-of-proteomes-extracted-through-collagen-based-stable-isotope-and-radiocarbon-dating-methods
#6
Caroline Wadsworth, Michael Buckley
Isotope analyses on "collagen" extracted from ancient bone have been routinely used for dietary and chronological inferences worldwide for decades. These methods involve the decalcification of biomineralized tissues with acid, often followed by processes to remove exogenous contaminants, and then gelatinization of what is often described as the "collagen" fraction. However, little is known about the relative content of collagen to the many other noncollagenous proteins (NCPs) potentially present. Some of these NCPs have great longevity in ancient bone, and some, for example, fetuin-A, are useful for obtaining better taxonomic information than collagen...
November 13, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29110340/effect-of-topical-fluoride-application-on-enamel-after-in-office-bleaching-as-evaluated-using-a-novel-hardness-tester-and-a-transverse-microradiography-method
#7
Mai Fukuyama, Chiharu Kawamoto, Pipop Saikaew, Yasuhiro Matsuda, Ricardo M Carvalho, Denis Selimovic, Hidehiko Sano
This study evaluated the effect of topical fluoride application on enamel hardness after in-office bleaching. Twelve human incisors were cut along the long axis, resulting in 24 halves used in four treatment groups (n = 6 in each group): (i) untreated group (C); (ii) in-office bleaching material (B); (iii) treatment with surface reaction-type prereacted glass-ionomer varnish after in-office bleaching (B+PRG); and (iv) treatment with acidulated phosphate fluoride solution after bleaching (B+F). All specimens were subjected to pH-cycling for 4 wk...
November 7, 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/29076527/a-comparative-analysis-of-microscopic-alterations-in-modern-and-ancient-undecalcified-and-decalcified-dry-bones
#8
Valentina Caruso, Marco Cummaudo, Emanuela Maderna, Annalisa Cappella, Giorgio Caudullo, Valentina Scarpulla, Cristina Cattaneo
OBJECTIVES: The present study aims to evaluate the preservation of the microstructure of skeletal remains collected from four different known burial sites (archaeological and contemporary). Histological analysis on undecalcified and decalcified thin sections was performed in order to assess which of the two techniques is more affected by taphonomic insults. MATERIALS AND METHODS: A histological analysis was performed on both undecalcified and decalcified thin sections of 40 long bones and the degree of diagenetic change was evaluated using transmitted and polarized light microscopy according to the Oxford Histological Index (OHI)...
October 27, 2017: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/29023217/visual-impairment-by-multiple-vascular-embolization-with-hydroxyapatite-particles
#9
Yayoi Marumo, Miki Hiraoka, Masato Hashimoto, Hiroshi Ohguro
We demonstrate a case of ocular impairment caused by a hydroxyapatite filler injection and review the prior literature on clinical presentations. A healthy woman, who received a hydroxyapatite filler injection into the glabella for nose augmentation suddenly had symptoms of nausea, diplopia, visual loss in the left eye, and impaired consciousness. Her left eye showed paresis of the inferior branch of the oculomotor nerve, conjunctival injection, cell infiltration in the anterior chamber, and multiple white spots in the nasal fundus...
October 12, 2017: Orbit
https://www.readbyqxmd.com/read/28958188/comparison-of-different-decalcification-methods-using-rat-mandibles-as-a-model
#10
Flavia M Savi, Gary I Brierly, Jeremy Baldwin, Christina Theodoropoulos, Maria A Woodruff
Selection of decalcification agents is an essential consideration when processing mineralized tissues because the integrity and immunohistochemical characteristics of the tissues may be affected. Here, we report results obtained from the decalcification of rat mandibles using 10% ethylenediaminetetraacetic acid (EDTA) at room temperature (RT), 10% EDTA at 37C, 5% nitric acid, and 10% formic acid at RT. Decalcification endpoints were determined by microcomputed tomography. Morphological preservation and antigenicity were evaluated by hematoxylin and eosin staining and immunohistochemistry...
September 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28929330/development-of-a-rapid-analysis-method-for-bone-resection-margins-for-oral-squamous-cell-carcinoma-by-immunoblotting
#11
Claudia Haase, Bernd Lethaus, Ruth Knüchel-Clarke, Frank Hölzle, Angela Cassataro, Till Braunschweig
The purpose of this proof-of-principle study was to develop a rapid and approachable method to analyse bone resection margins in patients with oral squamous cell carcinoma (OSCC) in an intraoperative setting, similar to assessing frozen sections of soft tissue. Bone excision and risk of remaining tumour cells could be minimised, thus improving reconstruction measures and facilitating convalescence. Frozen, sawed wafers of porcine bone artificially combined with porcine skin (simulating OSCC properties) were used to develop and evaluate a new molecular method: protein transfer from non-decalcified, sawed wafers onto a membrane stained by immunofluorescence (Tissue-ProtTrans)...
September 19, 2017: Head and Neck Pathology
https://www.readbyqxmd.com/read/28920546/blood-supply-and-vascularity-of-the-glenoid-labrum-its-clinical-implications
#12
Abduelmenem Alashkham, Abdulrahman Alraddadi, Paul Felts, Roger Soames
BACKGROUND: Tears of the glenoid labrum are common after dislocation of the glenohumeral joint. The outcome for healing or surgical reconstruction of the glenoid labrum relies on the extent of its vascularization. This study aims to evaluate the glenoid labrum blood supply and to determine its regional vascularity. MATERIALS AND METHODS: A total of 140 shoulders (30 male and 40 female cadavers) were examined: mean age 81.5 years, range 53-101 years. All blood vessels around the glenohumeral joint were dissected and recorded...
September 2017: Journal of Orthopaedic Surgery
https://www.readbyqxmd.com/read/28915544/mobilisation-of-toxic-trace-elements-under-various-beach-nourishments
#13
Iris R Pit, Stefan C Dekker, Tobias J Kanters, Martin J Wassen, Jasper Griffioen
To enhance protection and maintain wide beaches for recreation, beaches are replenished with sand: so-called beach nourishments. We compared four sites: two traditional beach nourishments, a mega beach nourishment and a reference without beach nourishment. Two sites contain calcareous-rich sand, whereas the other two sites have calcareous-poor sand. We aimed to understand hydrogeochemical processes to indicate factors critical for the mobility of trace elements at nourishments. We therefore analysed the chemical characteristics of sediment and pore water to ascertain the main drivers that mobilise toxic trace elements...
December 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28890234/calibration-of-a-lactic-acid-model-for-simulating-biofilm-induced-degradation-of-the-dentin-composite-interface
#14
Laikuan Zhu, Yuping Li, Carola A Carrera, Yung-Chung Chen, Mingyu Li, Alex Fok
OBJECTIVE: To verify and calibrate a chemical model for simulating the degradation of the dentin-composite interface induced by multi-species oral biofilms in vitro. METHODS: Dentin-composite disks (5-mm dia.×2-mm thick) were made from bovine incisor roots and filled with either Z100™ (Z100) or Filtek™ LS (LS) composite. The disks, which were covered with nail varnish, but with one of the dentin-composite margins exposed, were immersed in lactic acid solution at pH 4...
November 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28877070/effects-of-hydrochloric-acid-and-formic-acid-decalcification-on-breast-tumor-biomarkers-and-her2-fluorescence-in-situ-hybridization
#15
Beth Z Clark, Jennifer M Yoest, Agnieszka Onisko, David J Dabbs
Biomarker analysis of metastatic breast carcinoma (MBC) is routinely recommended by ASCO/CAP guidelines, and establishing a diagnosis of MBC often requires immunohistochemistry (IHC). The reliability of breast tumor biomarkers and breast-specific markers on decalcified tissues has not been extensively studied. We performed IHC studies on breast tumors exposed to hydrochloric acid (HCl) and formic acid (FA) decalcification solutions, and HER2 fluorescence in situ hybridization on a subset of these tumors to establish a protocol for handling bone specimens with suspicion for MBC...
September 4, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28873437/three-dimensional-hard-and-soft-tissue-imaging-of-the-human-cochlea-by-scanning-laser-optical-tomography-slot
#16
Nadine Tinne, Georgios C Antonopoulos, Saleh Mohebbi, José Andrade, Lena Nolte, Heiko Meyer, Alexander Heisterkamp, Omid Majdani, Tammo Ripken
The present study focuses on the application of scanning laser optical tomography (SLOT) for visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique which allows for tomographic imaging of the internal structure of transparent specimens. Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises decalcification, dehydration as well as optical clearing of the cochlea samples in toto...
2017: PloS One
https://www.readbyqxmd.com/read/28864350/immunohistochemical-analysis-of-nkx2-2-etv4-and-bcor-in-a-large-series-of-genetically-confirmed-ewing-sarcoma-family-of-tumors
#17
Isidro Machado, Akihiko Yoshida, José Antonio López-Guerrero, María Gema Nieto, Samuel Navarro, Piero Picci, Antonio Llombart-Bosch
Ewing sarcoma is an aggressive neoplasm of pediatric and adolescent patients. Immunohistochemistry (IHC) can be used to support the morphologic diagnosis of Ewing sarcoma family of tumors (ESFT) in a convincing clinical/radiological context. Although neither NKX2.2 nor CD99 alone are entirely specific, when combined, the diagnostic specificity is high. The aim of the present study was to investigate the IHC expression of NKX2.2, ETV4 and BCOR in a large series of genetically confirmed ESFT. The results for CD99 and CAV-1 immunoreactivity, and the histological and fusion gene subtypes were retrieved from our previous study...
August 25, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28856324/decalcification-of-a-heavily-calcified-common-femoral-artery-and-its-bifurcation-with-a-cavitron-ultrasonic-surgical-aspirator
#18
S Maeda, T Nakamura
INTRODUCTION: Surgical endarterectomy is the preferred method for treating occlusive disease of the common femoral artery (CFA). However, endarterectomy is not always straightforward in cases with heavily calcified plaque. To overcome this limitation, a new method for decalcification, which utilizes a Cavitron ultrasonic surgical aspirator (CUSA) has been developed. REPORT: The method involves full exposure of the calcified lesion. Following an arteriotomy, protruding calcification is removed using the CUSA, taking care to avoid vessel perforation...
2017: EJVES Short Reports
https://www.readbyqxmd.com/read/28833664/compound-specific-amino-acid-%C3%AE-15-n-values-in-archaeological-shell-assessing-diagenetic-integrity-and-potential-for-isotopic-baseline-reconstruction
#19
Nicole Misarti, Elizabeth Gier, Bruce Finney, Kelli Barnes, Matthew McCarthy
RATIONALE: Reconstructing stable isotope (SI) ratios at the base of paleo-food webs is often challenging. For coastal systems, the SI ratios of organic matter in archeological shell represents a possible solution, providing a direct record of primary consumer SI ratios in the littoral zone. However, shell is often porous, with organic compounds susceptible to diagenetic alteration or contamination. If molecular isotopic information is well preserved, compound-specific amino acid isotope analysis (CSI-AA) has the potential to provide direct proxies for baseline SI ratios, bypassing many contamination issues, and to allow assessment of the diagenetic state...
November 30, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28810293/-comparative-study-of-three-different-decalcifying-solutions-in-bone-metastasis-specimens-with-breast-cancer
#20
H Y Wu, T Wang, K L Chen, Z W Fan, X H Pu, B Zhang, X S Fan, F Q Meng
Objective: To investigate the optimal strategy for immunohistochemical (IHC) staining in bone metastasis specimens from breast cancer. Methods: Twenty-eight bone metastases specimens from breast cancers were divided into three groups and subjected to different decalcifying agents (group A-10% nitrate, group B-EDTA decalcification, and group C-imported decalcifying solution RapidCal). The effects of those on HE and IHC staining for Ki-67, ER, PR, GATA3, RANK, RANKL, HER2 and HER2 FISH results were assessed. Results: There were no significant differences among three groups in HE morphology and IHC staining...
August 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
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