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https://www.readbyqxmd.com/read/28506631/susceptibility-of-neuroblastoma-cells-to-rabies-virus-may-be-affected-by-passage-number
#1
Craig Pouliott, Michelle Dupuis, Kim Appler, Scott Brunt, Robert Rudd, April Davis
Maintaining a healthy, continuous immortalized cell line is essential for rabies laboratories that perform virus isolation assays and test for the presence of viral neutralizing antibodies. Individuals who routinely work with rabies virus, such as rabies laboratory employees, or those who may have a high potential for exposure to rabies virus, including veterinarians, should be tested for the presence of anti-rabies viral neutralizing antibodies (VNA) every 6 to 24 months, depending on potential exposure level...
May 12, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28490138/contamination-of-chinese-salted-fish-with-volatile-n-nitrosamines-as-determined-by-quechers-and-gas-chromatography-tandem-mass-spectrometry
#2
Yuesheng Qiu, Jie-Hua Chen, Weijun Yu, Pei Wang, Minxian Rong, Hong Deng
The QuEChERS sample preparation method and gas chromatography-tandem mass spectrometry were employed to determine nine volatile N-nitrosamines (VNAs) in Chinese salted fish. This method was validated by considering calibration plot linearity, selectivity, matrix effects, trueness, precision, limits of quantification and specificity. Fifty-four samples of Chinese salted fish obtained from five provinces were analyzed. The results indicated that the concentrations of one VNA, N-nitrosodimethylamine in 57.4% of the samples exceeded the acceptable limit (the China national standard value of 4μgkg(-1)), and total VNA contents in 68...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28490112/real-time-monitoring-of-sucrose-sorbitol-d-glucose-and-d-fructose-concentration-by-electromagnetic-sensing
#3
Supakorn Harnsoongnoen, Anuwat Wanthong
Magnetic sensing at microwave frequencies for real-time monitoring of sucrose, sorbitol, d-glucose and d-fructose concentrations is reported. The sensing element was designed based on a coplanar waveguide (CPW) loaded with a split ring resonator (SRR), which was fabricated on a DiClad 880 substrate with a thickness of 1.6mm and relative permittivity (εr) of 2.2. The magnetic sensor was connected to a Vector Network Analyzer (VNA) and the electromagnetic interaction between the samples and sensor was analyzed...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28417925/a-cost-effective-geodetic-strainmeter-based-on-dual-coaxial-cable-bragg-gratings
#4
Jihua Fu, Xu Wang, Tao Wei, Meng Wei, Yang Shen
Observations of surface deformation are essential for understanding a wide range of geophysical problems, including earthquakes, volcanoes, landslides, and glaciers. Current geodetic technologies, such as global positioning system (GPS), interferometric synthetic aperture radar (InSAR), borehole and laser strainmeters, are costly and limited in their temporal or spatial resolutions. Here we present a new type of strainmeters based on the coaxial cable Bragg grating (CCBG) sensing technology that provides cost-effective strain measurements...
April 12, 2017: Sensors
https://www.readbyqxmd.com/read/28317660/safety-tolerability-and-efficacy-of-intradermal-rabies-immunization-with-debioject%C3%A2
#5
Paul Vescovo, Nils Rettby, Nirinarilala Ramaniraka, Julie Liberman, Karen Hart, Astrid Cachemaille, Laurent-Dominique Piveteau, Reto Zanoni, Pierre-Alexandre Bart, Giuseppe Pantaleo
In a single-center study, 66 healthy volunteers aged between 18 and 50years were randomized to be immunized against rabies with three different injection routes: intradermal with DebioJect™ (IDJ), standard intradermal with classical needle (IDS), also called Mantoux method, and intramuscular with classical needle (IM). "Vaccin rabique Pasteur®" and saline solution (NaCl 0.9%) were administered at D0, D7 and D28. Antigen doses for both intradermal routes were 1/5 of the dose for IM. Tolerability, safety and induced immunogenicity of IDJ were compared to IDS and IM routes...
March 27, 2017: Vaccine
https://www.readbyqxmd.com/read/28151079/exposure-to-lyssaviruses-in-bats-of-the-democratic-republic-of-the-congo
#6
Lem's N Kalemba, Michael Niezgoda, Amy T Gilbert, Jeffrey B Doty, Ryan M Wallace, Jean M Malekani, Darin S Carroll
Lyssavirus infections in the Democratic Republic of Congo are poorly documented. We examined 218 bats. No lyssavirus antigens were detected but Lagos bat virus (LBV) neutralizing antibodies (VNA) were detected in Eidolon helvum and Myonycteris torquata . Four samples with LBV VNA reacted against Shimoni bat virus.
April 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28113238/channel-modeling-of-miniaturized-battery-powered-capacitive-human-body-communication-systems
#7
Jiwoong Park, Harinath Garudadri, Patrick P Mercier
The purpose of this contribution is to estimate the path loss of capacitive human body communication (HBC) systems under practical conditions. METHODS: Most prior work utilizes large, grounded instruments to perform path loss measurements, resulting in overly optimistic path loss estimates for wearable HBC devices. In this paper, small battery-powered transmitter and receiver devices are implemented to measure path loss under realistic assumptions. A hybrid electrostatic-FEM simulation model is presented that validates measurements and enables rapid and accurate characterization of future capacitive HBC systems...
April 29, 2016: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28100620/overexpression-of-interleukin-7-extends-the-humoral-immune-response-induced-by-rabies-vaccination
#8
Yingying Li, Ming Zhou, Zhaochen Luo, Yachun Zhang, Min Cui, Huanchun Chen, Zhen F Fu, Ling Zhao
Rabies continues to present a public health threat in most countries of the world. The most efficient way to prevent and control rabies is to implement vaccination programs for domestic animals. However, traditional inactivated vaccines used in animals are costly and have relatively low efficiency, which impedes their extensive use in developing countries. There is, therefore, an urgent need to develop single-dose and long-lasting rabies vaccines. However, little information is available regarding the mechanisms underlying immunological memory, which can broaden humoral responses following rabies vaccination...
April 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28089546/expression-of-interleukin-6-by-a-recombinant-rabies-virus-enhances-its-immunogenicity-as-a-potential-vaccine
#9
Jun Luo, Boyue Zhang, Yuting Wu, Qin Tian, Jing Zhao, Ziyu Lyu, Qiong Zhang, Mingzhu Mei, Yongwen Luo, Xiaofeng Guo
Several studies have confirmed that interleukin-6 (IL6) mediates multiple biological effects that enhance immune responses when used as an adjuvant. In the present study, recombinant rabies virus (RABV) expressing canine IL6 (rHEP-CaIL6) was rescued and its pathogenicity and immunogenicity were investigated in mice. We demonstrated that mice received a single intramuscular immunization with rHEP-CaIL6 showed an earlier increase and higher maximum titres of virus-neutralizing antibody (VNA) as well as anti-RABV antibodies compared with mice immunized with the parent strain...
January 12, 2017: Vaccine
https://www.readbyqxmd.com/read/28075375/an-approach-to-biometric-verification-based-on-human-body-communication-in-wearable-devices
#10
Jingzhen Li, Yuhang Liu, Zedong Nie, Wenjian Qin, Zengyao Pang, Lei Wang
In this paper, an approach to biometric verification based on human body communication (HBC) is presented for wearable devices. For this purpose, the transmission gain S21 of volunteer's forearm is measured by vector network analyzer (VNA). Specifically, in order to determine the chosen frequency for biometric verification, 1800 groups of data are acquired from 10 volunteers in the frequency range 0.3 MHz to 1500 MHz, and each group includes 1601 sample data. In addition, to achieve the rapid verification, 30 groups of data for each volunteer are acquired at the chosen frequency, and each group contains only 21 sample data...
January 10, 2017: Sensors
https://www.readbyqxmd.com/read/27997343/rabies-pre-exposure-prophylaxis-elicits-long-lasting-immunity-in-humans
#11
Karen L Mansfield, Nick Andrews, Hooman Goharriz, Trudy Goddard, Lorraine M McElhinney, Kevin E Brown, Anthony R Fooks
Despite the availability of safe and effective human vaccines, rabies remains a global threat, with an estimated 60,000 human deaths annually attributed to rabies. Pre-exposure prophylaxis against rabies infection is recommended for travelers to countries where rabies is endemic, and also for those with a higher risk of exposure. In this study, the rabies-specific neutralising antibody responses in a cohort of rabies-vaccinated recipients over a period of twenty years have been assessed. In particular, the antibody response to primary vaccinations and boosters, and the waning of antibody post primary vaccination and post booster were investigated...
November 21, 2016: Vaccine
https://www.readbyqxmd.com/read/27902338/recombinant-rabies-virus-expressing-il-21-enhances-immunogenicity-through-activation-of-t-follicular-helper-cells-and-germinal-centre-b-cells
#12
Yajing Zhang, Ming Zhou, Zhao Wang, Jie Yang, Mingming Li, Kunlun Wang, Min Cui, Huanchun Chen, Zhen F Fu, Ling Zhao
Previous studies have demonstrated that the lack of interleukin-21 (IL-21) signalling could affect specific antibody induction after rabies vaccination. Here, to further investigate the over-expression of IL-21 on the immunogenicity of rabies virus (RABV), a recombinant RABV expressing murine IL-21, designated LBNSE-IL21, was constructed and evaluated in a mouse model. It was found that in mice immunized with LBNSE-IL21, there was a substantial increase in the number of T follicular helper cells and germinal centre B cells but no enhancement of dendritic cell activation...
December 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27897209/lightweight-nife2o4-with-controllable-3d-network-structure-and-enhanced-microwave-absorbing-properties
#13
Fen Wang, Xing Wang, Jianfeng Zhu, Haibo Yang, Xingang Kong, Xiao Liu
3D network structure NiFe2O4 was successfully synthesized by a templated salt precipitation method using PMMA colloid crystal as templates. The morphology, phase composition and microwave absorbing properties of as-prepared samples were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), vector network analyzer (VNA), and so on. The results revealed that the 3D network structure was configurated with smooth spherical walls composed of NiFe2O4 nanocrystals and their pore diameters being in the range of 80-250 nm...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27863040/a-comparative-study-of-the-rapina-and-the-virus-neutralizing-test-rffit-for-the-estimation-of-antirabies-neutralizing-antibody-levels-in-dog-samples
#14
D L Manalo, K Yamada, I Watanabe, M E G Miranda, S M D Lapiz, E Tapdasan, W Petspophonsakul, S Inoue, P Khawplod, A Nishizono
The mass vaccination of dogs against rabies is a highly rational strategy for interrupting the natural transmission of urban rabies. According to the World Organization for Animal Health (OIE) and the World Health Organization (WHO), the immunization of at least 70% of the total dog population minimizes the risk of endemic rabies. Knowledge of the virus-neutralizing antibody (VNA) level against the rabies virus (RABV) is required to evaluate protective immunity and vaccine coverage of dogs in the field. The rapid focus fluorescent inhibition test (RFFIT) and the fluorescent antibody virus neutralization (FAVN) test are recommended by OIE and WHO to determine the VNA levels in serum...
November 12, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/27852854/a-novel-rabies-vaccine-expressing-cxcl13-enhances-humoral-immunity-by-recruiting-both-t-follicular-helper-and-germinal-center-b-cells
#15
Zhao Wang, Mingming Li, Ming Zhou, Yajing Zhang, Jie Yang, Yandi Cao, Kunlun Wang, Min Cui, Huanchun Chen, Zhen F Fu, Ling Zhao
Rabies remains a public health threat in most parts of the world, and approximately 99% of the cases are transmitted by dogs. There is an urgent need to develop an efficacious and affordable vaccine to control canine-transmitted rabies in developing countries. Our previous studies demonstrate that overexpression of chemokines/cytokines such as CCL-3 (MIP-1α) and granulocyte-macrophage colony-stimulating factor (GM-CSF) can enhance the immunogenicity of rabies vaccines. In the present study, the chemokine CXCL13 was inserted into the genome of the recombinant rabies virus (rRABV) strain LBNSE, and the effect of the chemokine CXCL13 on the immunogenicity of RABV was investigated...
February 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27722994/comparison-of-immune-responses-to-attenuated-rabies-virus-and-street-virus-in-mouse-brain
#16
Fa-Ming Miao, Shou-Feng Zhang, Shu-Chao Wang, Ye Liu, Fei Zhang, Rong-Liang Hu
Rabies is a lethal neurological disease caused by the neurotropic rabies virus (RABV). To investigate the innate immune response in the brain during rabies infection, key gene transcripts indicative of innate immunity in a mouse model system were measured using real-time RT-PCR. Mice were infected via the intracerebral or intramuscular route with either attenuated rabies virus (SRV9) or pathogenic rabies virus (BD06). Infection with SRV9 resulted in the early detection of viral replication and the rapid induction of innate immune response gene expression in the brain...
January 2017: Archives of Virology
https://www.readbyqxmd.com/read/27587141/biasing-vector-network-analyzers-using-variable-frequency-and-amplitude-signals
#17
J E Nobles, V Zagorodnii, A Hutchison, Z Celinski
We report the development of a test setup designed to provide a variable frequency biasing signal to a vector network analyzer (VNA). The test setup is currently used for the testing of liquid crystal (LC) based devices in the microwave region. The use of an AC bias for LC based devices minimizes the negative effects associated with ionic impurities in the media encountered with DC biasing. The test setup utilizes bias tees on the VNA test station to inject the bias signal. The square wave biasing signal is variable from 0...
August 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27539858/a-diverse-set-of-single-domain-antibodies-vhhs-against-the-anthrax-toxin-lethal-and-edema-factors-provides-a-basis-for-construction-of-a-bispecific-agent-that-protects-against-anthrax-infection
#18
Catherine E Vrentas, Mahtab Moayeri, Andrea B Keefer, Allison J Greaney, Jacqueline Tremblay, Danielle O'Mard, Stephen H Leppla, Charles B Shoemaker
Infection with Bacillus anthracis, the causative agent of anthrax, can lead to persistence of lethal secreted toxins in the bloodstream, even after antibiotic treatment. VHH single-domain antibodies have been demonstrated to neutralize diverse bacterial toxins both in vitro and in vivo, with protein properties such as small size and high stability that make them attractive therapeutic candidates. Recently, we reported on VHHs with in vivo activity against the protective antigen component of the anthrax toxins...
October 7, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27534615/gene-signatures-associated-with-adaptive-humoral-immunity-following-seasonal-influenza-a-h1n1-vaccination
#19
I G Ovsyannikova, H M Salk, R B Kennedy, I H Haralambieva, M T Zimmermann, D E Grill, A L Oberg, G A Poland
This study aimed to identify gene expression markers shared between both influenza hemagglutination inhibition (HAI) and virus-neutralization antibody (VNA) responses. We enrolled 158 older subjects who received the 2010-2011 trivalent inactivated influenza vaccine. Influenza-specific HAI and VNA titers and mRNA-sequencing were performed using blood samples obtained at Days 0, 3 and 28 post vaccination. For antibody response at Day 28 versus Day 0, several gene sets were identified as significant in predictive models for HAI (n=7) and VNA (n=35) responses...
December 2016: Genes and Immunity
https://www.readbyqxmd.com/read/27515260/chimeric-rabies-glycoprotein-with-a-transmembrane-domain-and-cytoplasmic-tail-from-newcastle-disease-virus-fusion-protein-incorporates-into-the-newcastle-disease-virion-at-reduced-levels
#20
Gui Mei Yu, Shu Long Zu, Wei Wei Zhou, Xi Jun Wang, Lei Shuai, Xue Lian Wang, Jin Ying Ge, Zhi Gao Bu
Rabies is still an important world-wide health problem. Newcastle disease virus (NDV) has been developed as a vaccine vector in animals using a reverse genetics system. Previously, our group generated a recombinant NDV (LaSota strain) expressing the complete rabies G protein (RVG), named rL-RVG. In this study, we constructed a variant, rL-RVGTM, which expresses a chimeric rabies G protein RVGTM containing the ectodomain of RVG and the transmembrane domain(TM) and cytoplasmic tail (CT) from the NDV fusion glycoprotein to study the function of RVG's TM and CT...
August 10, 2016: Journal of Veterinary Science
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