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https://www.readbyqxmd.com/read/28644936/the-deltoid-lift-a-comparison-study-of-exposure-area-in-proximal-humeral-approaches
#1
Michael Ting, Nicholas Kusnezov, John Dunn, Alok K Dwivedi, Miguel A Pirela-Cruz
BACKGROUND: Adequate exposure of the proximal humerus is necessary to address atypical or complex proximal humerus fractures and orthopedic tumors. Surgical management may be difficult through existing approaches due to their limited nature and the delicate neurovascular anatomy of the shoulder. The deltoid lift, a previously described extensile approach, can be incorporated into the surgeon's armamentarium as an alternative exposure to the proximal humerus. The objective of this study was to quantify and compare the humeral exposure achieved through the deltoid lift with the standard direct lateral deltoid-splitting, anterolateral acromial, and deltopectoral approaches in terms of surface area and exposure of key anatomic landmarks...
July 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28643130/retreatability-of-three-calcium-silicate-containing-sealers-and-one-epoxy-resin-based-root-canal-sealer-with-four-different-root-canal-instruments
#2
David Donnermeyer, Clarissa Bunne, Edgar Schäfer, Till Dammaschke
OBJECTIVES: The objective of the study was to compare the retreatability of three calcium silicate-containing sealers (BioRoot RCS, MTA Fillapex, Endo C.P.M.) and an epoxy resin-based sealer (AH Plus) with different root canal instruments (Hedström files, Reciproc R40, Mtwo retreatment file R 25/.05 + Mtwo 40/.06, and F6 SkyTaper) concerning sealer remnants and retreatment time. MATERIALS AND METHODS: Root canals of 192 teeth were instrumented with Reciproc R40...
June 22, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28637033/quantitative-swept-source-optical-coherence-tomography-of-early-enamel-erosion-in-vivo
#3
Rupert S Austin, Maisalamah Haji Taha, Frederic Festy, Richard Cook, Manoharan Andiappan, Juliana Gomez, Iain A Pretty, Rebecca Moazzez
Swept-source optical coherence tomography (SS-OCT) shows potential for the in vivo quantitative evaluation of micro-structural enamel surface phenomena occurring during early erosive demineralization. This randomized controlled single-blind cross-over clinical study aimed to evaluate the use of SS-OCT for detecting optical changes in the enamel of 30 healthy volunteers subjected to orange juice rinsing (erosive challenge) in comparison to mineral water rinsing (control), according to wiped and non-wiped enamel surface states...
June 22, 2017: Caries Research
https://www.readbyqxmd.com/read/28623652/micro-thin-layer-chromatography-fingerprints-of-selected-basil-species-and-their-chemometric-analysis
#4
Mirosław A Hawrył, Ryszard Świeboda, Anna Hawrył, Małgorzata Niemiec, Monika Waksmundzka-Hajnos
The dried aerial parts of 12 plants of Ocimum spp. were extracted with the Soxhlet apparatus usingdichloromethane and methanol as solvents. A micro-TLC system with silica and a normal-phase solvent system (propan-2-ol-n-heptane-formic acid) was used for the chemometric analysis of 12 selected basil methanolic extracts. Some indices of similarity (Pearson's correlation coefficient, determination coefficient, congruence coefficient, and Euclidean, Manhattan (city-block), Chebyshev, and Hausdorff distances) were calculated on the basis of thin-layer chromatograms using ImageJ software...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28616362/assessing-the-accuracy-of-foveal-avascular-zone-measurements-using-optical-coherence-tomography-angiography-segmentation-and-scaling
#5
Rachel Linderman, Alexander E Salmon, Margaret Strampe, Madia Russillo, Jamil Khan, Joseph Carroll
PURPOSE: The foveal avascular zone (FAZ) is altered in numerous diseases. We assessed factors (axial length, segmentation method, age, sex) impacting FAZ measurements from optical coherence tomography (OCT) angiography images. METHODS: We recruited 116 Caucasian subjects without ocular disease, and acquired two 3 × 3 mm AngioVue scans per each right eye (232 total scans). In images of the superficial plexus, the FAZ was segmented using the AngioVue semiautomatic nonflow measurement tool and ImageJ manual segmentation...
June 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/28610577/coronary-angiogenic-effect-of-long-term-administration-of-nigella-sativa
#6
Lubna I Al Asoom
BACKGROUND: Coronary angiogenesis is one of the preferable adaptive responses of aerobic training. Previous studies found inotropic and hypertrophic cardiac effects for long-term administration of Nigella sativa (NS), but no studies have explored its coronary angiogenic effect. The present study compared the effect of long-term NS- administration and exercise training on the induction of coronary angiogenesis. METHOD: Fifteen adult male Wistar rats were divided into three groups: control, NS-fed, and exercise-trained (Ex)...
June 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28596679/oxidative-stress-induced-mitochondrial-dysfunction-in-a-normal-colon-epithelial-cell-line
#7
Nandakumar Packiriswamy, Kari F Coulson, Susan J Holcombe, Lorraine M Sordillo
AIM: To determine how a normal human colon cell line reacts to microbial challenge as a way to study oxidative stress-induced responses associated with inflammatory bowel disease. METHODS: Normal human colon epithelial cells (ATCC(®) CRL.1790™) were stimulated with either heat killed E. coli or heat killed murine cecal contents (HKC) and examined for several relevant biomarkers associated with inflammation and oxidative stress including cytokine production, mitochondrial autophagy and oxidant status...
May 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28594001/a-basic-tool-for-background-and-shading-correction-of-optical-microscopy-images
#8
Tingying Peng, Kurt Thorn, Timm Schroeder, Lichao Wang, Fabian J Theis, Carsten Marr, Nassir Navab
Quantitative analysis of bioimaging data is often skewed by both shading in space and background variation in time. We introduce BaSiC, an image correction method based on low-rank and sparse decomposition which solves both issues. In comparison to existing shading correction tools, BaSiC achieves high-accuracy with significantly fewer input images, works for diverse imaging conditions and is robust against artefacts. Moreover, it can correct temporal drift in time-lapse microscopy data and thus improve continuous single-cell quantification...
June 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28572833/development-of-high-throughput-methods-to-screen-disease-caused-by-rhizoctonia-solani-ag-2-1-in-oilseed-rape
#9
Fryni Drizou, Neil S Graham, Toby J A Bruce, Rumiana V Ray
BACKGROUND: Rhizoctonia solani (Kühn) is a soil-borne, necrotrophic fungus causing damping off, root rot and stem canker in many cultivated plants worldwide. Oilseed rape (OSR, Brassica napus) is the primary host for anastomosis group (AG) 2-1 of R. solani causing pre- and post-emergence damping-off resulting in death of seedlings and impaired crop establishment. Presently, there are no known resistant OSR genotypes and the main methods for disease control are fungicide seed treatments and cultural practices...
2017: Plant Methods
https://www.readbyqxmd.com/read/28570534/quantification-of-endosome-and-lysosome-motilities-in-cultured-neurons-using-fluorescent-probes
#10
Fuminori Tsuruta, Tomomi Okajima, Sarasa Yano, Tomoki Chiba
In the brain, membrane trafficking systems play important roles in regulating neuronal functions, such as neuronal morphology, synaptic plasticity, survival, and glial communications. To date, numerous studies have reported that defects in these systems cause various neuronal diseases. Thus, understanding the mechanisms underlying vesicle dynamics may provide influential clues that could aid in the treatment of several neuronal disorders. Here, we describe a method for quantifying vesicle motilities, such as motility distance and rate of movement, using a software plug-in for the ImageJ platform...
May 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28566114/effects-of-ovarian-disaggregation-on-adult-murine-follicle-yield-and-viability
#11
Fiona Young, John Drummond, Emma Akers, Louise Bartle, David Kennedy, Mohammad Asaduzzaman
Follicles are isolated from ovaries for numerous reasons, including IVM, but adult murine yields are <2 folliclesmg-1. The aim of the present study was to optimise ovarian disaggregation and develop methods applicable to the rapid screening of follicle viability. Ovaries from adult mice (n=7) were halved and disaggregated mechanically, or by using collagenase IV (Col-IV; 590UmL-1) or animal origin-free collagenase IV (AOF) at 590 or 1180UmL-1. Isolated follicles were stained with 4',6'-diamidino-2-phenylindole (DAPI; nuclei), chloromethyl-X-rosamine (CMXRos; mitochondria) or fluorescein isothiocyanate-conjugated anti-α-tubulin antibody...
June 1, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28561033/smooth-2d-manifold-extraction-from-3d-image-stack
#12
Asm Shihavuddin, Sreetama Basu, Elton Rexhepaj, Felipe Delestro, Nikita Menezes, Séverine M Sigoillot, Elaine Del Nery, Fekrije Selimi, Nathalie Spassky, Auguste Genovesio
Three-dimensional fluorescence microscopy followed by image processing is routinely used to study biological objects at various scales such as cells and tissue. However, maximum intensity projection, the most broadly used rendering tool, extracts a discontinuous layer of voxels, obliviously creating important artifacts and possibly misleading interpretation. Here we propose smooth manifold extraction, an algorithm that produces a continuous focused 2D extraction from a 3D volume, hence preserving local spatial relationships...
May 31, 2017: Nature Communications
https://www.readbyqxmd.com/read/28560481/fluorescence-to-highlight-the-urethra-a-human-cadaveric-study
#13
T G Barnes, M Penna, R Hompes, C Cunningham
BACKGROUND: Urethral injury is a complication feared by surgeons performing transanal TME (TaTME) or abdominoperineal excision (APE) procedures. Injury during TaTME occurs when the prostate is inadvertently mobilised or as a direct injury similar to the direct injury during the perineal dissection of APE procedures. We performed a proof of principle study to assess the feasibility of using indocyanine green (ICG) to fluoresce the urethra in human cadavers. METHODS: Indocyanine green at varying doses was mixed with Instillagel and infiltrated into the urethra of male human cadavers...
May 30, 2017: Techniques in Coloproctology
https://www.readbyqxmd.com/read/28558328/microscopic-evaluation-of-the-ventricular-tissue-using-stereological-and-voronoi-tessellation-methods-application-on-doxorubicin-induced-cardiotoxicity-in-rats
#14
Ali Rafati, Zahra Jafarinezhad, Saied Karbalay-Doust, Leila Hosseini, Ali Noorafshan
There is limited quantitative microscopic information concerning the three-dimensional arrangement of cardiomyocytes and microvessels in cardiotoxic condition. The aim of this study was to evaluate the cardiac tissue of rats under different experimental conditions using modern second-order stereology and Voronoi tessellation techniques to show the spatial arrangement of the cardiomyocytes and microvessels. Sprague-Dawley rats were randomly divided into two groups (n=6). The control group received PBS and the doxorubicin (DOX) group received 4mg/kg DOX on days 1, 8, 15, and 22...
May 24, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28545510/effect-of-bone-marrow-derived-mesenchymal-stem-cells-and-stem-cell-supernatant-on-equine-corneal-wound-healing-in-vitro
#15
Amanda B Sherman, Brian C Gilger, Alix K Berglund, Lauren V Schnabel
BACKGROUND: We aimed to determine and compare the in vitro effects of autologous bone marrow-derived mesenchymal stem cells (BM-MSCs) and mesenchymal stem cell supernatant (MSC-Sp) on the wound healing capacity of equine corneal fibroblasts using a scratch assay. METHODS: Bone marrow aspirates and eyes were collected from normal, euthanized horses with subsequent isolation and culture of BM-MSCs and corneal stromal cells. Corneal stromal cells were culture-expanded in the culture well of transwell plates and then treated with an autologous BM-MSC suspension (dose: 2...
May 25, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28543185/iron-oxide-nanoparticles-decrease-nuclear-fractal-dimension-of-buccal-epithelial-cells-in-a-time-dependent-manner
#16
D Nikolovski, S Dugalic, I Pantic
In this paper, we present results that iron oxide nanoparticles (IONPs) induce time-dependent structural changes in nuclei of buccal epithelial cells. The cells were treated with magnetite, Fe3 O4 nanoparticles (spherical shape, diameter 80-100 nanometres). The digital micrographs of the nuclei were made in 3 different time points: 15, 30 and 60 min after the treatment with IONPs, as well as in the control cells. A total of 120 nuclear structures (30 per sample) were analysed. Fractal analysis of nuclei was done in ImageJ software of the National Institutes of Health, (Bethesda, MD, USA)...
May 23, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28540178/scanometry-as-microplate-reader-for-high-throughput-method-based-on-dpph-dry-reagent-for-antioxidant-assay
#17
Mochammad Amrun Hidayat, Aulia Fitri, Bambang Kuswandi
The stable chromogenic radical 1,1'-diphenyl-2-picrylhydrazyl (DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular flatbed scanner was used as microplate reader to obtain analytical parameters for antioxidant assay using one-shot optical sensors as scanometry technique. Variables affecting the acquisition of the images were optimized and the analytical parameters are obtained from an area of the sensing zone inside microwell using the average luminosity of the sensing zone captured as the mean of red, green, and blue (RGB) value using ImageJ(®) program...
May 2017: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/28540005/post-photorefractive-keratectomy-pain-and-corneal-sub-basal-nerve-density
#18
Masoumeh Mohebbi, Amin Rafat-Nejad, Seyed-Farzad Mohammadi, Kosar Asna-Ashari, Maryam Kasiri, Saeed Heidari-Keshel, Farshad Askarizadeh
PURPOSE: The perceived and reported pain of patients receiving photorefractive keratectomy (PRK) widely varies. We assessed the potential role of the subbasal nerve plexus density as a predictor of postoperative pain level. Consecutive patients scheduled to undergo PRK at the Refractive Surgery Clinic of Farabi Eye Hospital, Tehran, were approached. METHODS: Forty-nine myopic left eyes from 49 patients who consented to undergo scanning slit confocal microscopy assessments preoperatively were included...
April 2017: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/28536976/memory-and-learning-dysfunction-following-copper-toxicity-biochemical-and-immunohistochemical-basis
#19
Jayantee Kalita, Vijay Kumar, Usha K Misra, Himangsu K Bora
The prototype disease of Cu toxicity in human is Wilson disease, and cognitive impairment is the presenting symptom of it. There is no study correlating Cu-induced excitotoxicity, apoptosis, and astrocytic reaction with memory dysfunction. We report excitotoxicity, apoptosis, and astrocytic reaction of the hippocampus and frontal cortex with memory dysfunction in rat model of Cu toxicity. Thirty-six rats were divided into group I (control) and group II (100 mg/kgBwt/day CuSO4 orally). Y-maze was performed for memory and learning at 0, 30, 60, and 90 days...
May 23, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28535911/validation-of-a-free-software-for-unsupervised-assessment-of-abdominal-fat-in-mri
#20
Michele Maddalo, Ivan Zorza, Stefano Zubani, Giorgio Nocivelli, Giulio Calandra, Pierantonio Soldini, Lorella Mascaro, Roberto Maroldi
PURPOSE: To demonstrate the accuracy of an unsupervised (fully automated) software for fat segmentation in magnetic resonance imaging. The proposed software is a freeware solution developed in ImageJ that enables the quantification of metabolically different adipose tissues in large cohort studies. METHODS: The lumbar part of the abdomen (19cm in craniocaudal direction, centered in L3) of eleven healthy volunteers (age range: 21-46years, BMI range: 21.7-31.6kg/m(2)) was examined in a breath hold on expiration with a GE T1 Dixon sequence...
May 2017: Physica Medica: PM
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