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https://www.readbyqxmd.com/read/28205389/growth-inhibition-of-formed-corneal-neovascularization-following-fosaprepitant-treatment
#1
Fabio Bignami, Anna Lorusso, Paolo Rama, Giulio Ferrari
PURPOSE: The aim of this study was to test the efficacy of Neurokinin-1 Receptor (NK-1R) antagonist -Fosaprepitant- in inducing regression of established corneal neovascularization (CNV). METHODS: Twenty C57BL/6 mice underwent alkali burn. Seven days later, when corneal neovessels had developed, they received Fosaprepitant 10 mg/ml, administered topically six times a day in the right eye for 10 days. In parallel, a group of 20 causticated mice was treated with normal saline, as control...
February 15, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28196606/imaging-and-cell-count-in-cleared-intact-cochlea-in-the-mongolian-gerbil-using-laser-scanning-confocal-microscopy
#2
M Risoud, J Sircoglou, G Dedieu, M Tardivel, C Vincent, N-X Bonne
OBJECTIVES: To draw up a clearing protocol for Mongolian gerbil cochlea, and to assess the feasibility of quantifying and analyzing 3D cell architecture in the transparent cochleae. MATERIALS AND METHODS: Freshly dissected inner ears were prepared on a 13-day protocol: fixation, microdissection, post-fixation, decalcification, pretreatment (signal enhancement, permeabilization and blocking), fluorescent labeling (indirect immunolabeling and direct labeling), dehydration, clearing in Spalteholz solution (MSBB: methyl salicylate and benzyl benzoate) and mounting...
February 9, 2017: European Annals of Otorhinolaryngology, Head and Neck Diseases
https://www.readbyqxmd.com/read/28196271/dose-distribution-at-the-bragg-peak-dose-measurements-using-ebt-and-rtqa-gafchromic-film-set-at-two-positions-to-the-central-beam-axis
#3
Borowicz Dorota Maria, Malicki Julian, Mytsin Gennady, Shipulin Konstantin
AIM: To evaluate the impact of radiochromic film positioning relative to the central beam axis (CAX) in proton beam therapy. Secondarily, to compare the dosimetric measurements obtained by RTQA and EBT film and to compare these to the doses calculated by the treatment planning system (TPS). METHODS: EBT and RTQA dosimetric radiochromic films were immersed in a water phantom and irradiated with a proton beam. The films were placed parallel to the CAX and at a 5º angle on the horizontal plane to assess the effect of film inclination on Bragg peak profiles...
February 14, 2017: Medical Physics
https://www.readbyqxmd.com/read/28186647/technical-note-fully-automated-analysis-of-jaszczak-phantom-measurements-as-part-of-routine-spect-quality-control
#4
Albert Hirtl, Helmar Bergmann, Barbara Knäusl, Thomas Beyer, Michael Figl, Johann Hummel
PURPOSE: Inspection and quantitative validation of tomographic imaging properties of SPECT systems, i.e. spatial resolution, contrast and inhomogeneity must be performed in regular intervals. Typically, the modular Jaszczak phantom is used for that purpose, since it offers the possibility to investigate all three system properties with a single measurement. The interpretation of the measurement is performed visually, thus, being insensitive to subtle changes in system performance. To overcome this limitation, a fully-automated software for the objective analysis of Jaszczak phantom measurements is proposed here...
February 10, 2017: Medical Physics
https://www.readbyqxmd.com/read/28185265/teres-major-muscle-insertion-footprint
#5
Malte Dancker, Simon Lambert, Erich Brenner
Teres major muscle (TM) and latissimus dorsi muscle (LD) are frequently used in muscle transfers around the shoulder girdle. Some authors have suggested harvesting techniques in which the muscle is detached in continuity with a bone segment. Information on the bony attachment footprint of these muscles is lacking. The purpose of this study was to investigate the region of attachment of the TM to facilitate safe and complete harvesting with a bone segment where it is indicated, and to determine the relationship of the TM footprint with that of the LD...
February 9, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28185184/acquisition-and-post-processing-of-immunohistochemical-images
#6
Jerry Sedgewick
Augmentation of digital images is almost always a necessity in order to obtain a reproduction that matches the appearance of the original. However, that augmentation can mislead if it is done incorrectly and not within reasonable limits. When procedures are in place for insuring that originals are archived, and image manipulation steps reported, scientists not only follow good laboratory practices, but avoid ethical issues associated with post processing, and protect their labs from any future allegations of scientific misconduct...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28181789/a-novel-image-processing-technique-for-three-dimensional-volumetric-analysis-of-severely-resorbed-alveolar-sockets-with-cone-beam-computed-tomography
#7
Valeria Manavella, Federica Romano, Federica Garrone, Mara Terzini, Cristina Bignardi, Mario Aimetti
BACKGROUND: The aim of this study was to present and validate a novel procedure for the quantitative volumetric assessment of extraction sockets that combines Cone-Beam Computed Tomography (CBCT) and image processing techniques. METHODS: The CBCT dataset of 9 severely resorbed extraction sockets was analyzed by means of two image processing software, Image J and Mimics, using manual and automated segmentation techniques. They were also applied on 5 mm spherical aluminum markers of known volume and on a polyvinyl chloride model of one alveolar socket scanned with Micro-CT to test the accuracy...
February 9, 2017: Minerva Stomatologica
https://www.readbyqxmd.com/read/28177210/noise-reduction-method-for-quantifying-nanoparticle-light-scattering-in-low-magnification-dark-field-microscope-far-field-images
#8
Dali Sun, Jia Fan, Chang Liu, Yang Liu, Yang Bu, Christopher J Lyon, Ye Hu
Nanoparticles have become a powerful tool for cell imaging and biomolecule, cell and protein interaction studies, but are difficult to rapidly and accurately measure in most assays. Dark-field microscope (DFM) image analysis approaches used to quantify nanoparticles require high-magnification near-field (HN) images that are labor intensive due to a requirement for manual image selection and focal adjustments needed when identifying and capturing new regions of interest. Low-magnification far-field (LF) DFM imagery is technically simpler to perform but cannot be used as an alternate to HN-DFM quantification, since it is highly sensitive to surface artifacts and debris that can easily mask nanoparticle signal...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28166432/quantitative-histochemistry-for-macrophage-biodistribution-on-mice-liver-and-spleen-after-the-administration-of-a-pharmacological-relevant-dose-of-polyacrylic-acid-coated-iron-oxide-nanoparticles
#9
Daniela Rodrigues, Marisa Freitas, Vera Marisa Costa, M Arturo Lopez-Quintela, José Rivas, Paulo Freitas, Félix Carvalho, Eduarda Fernandes, Paula Silva
Understanding in vivo biodistribution of iron oxide nanoparticles (IONs), and the involvement of the phagocyte system in this process, is crucial for the assessment of their potential health risk. In the present study, the histochemical expression of iron in spleen and liver sections of CD-1 mice (aged 8 weeks) was quantified, 24 hours after intravenous administration of polyacrylic acid-coated IONs (PAA-coated IONs) (8, 20, 50 mg/kg). Organ sections were stained with Perls' Prussian blue for iron detection, followed by the quantification of iron deposition with ImageJ software...
February 6, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28150202/detecting-sonolysis-of-polyethylene-glycol-upon-functionalizing-carbon-nanotubes
#10
Ruhung Wang, Vasanth S Murali, Rockford Draper
Polyethylene glycol (PEG) and related polymers are often used in the solubilization and noncovalent functionalization of carbon nanomaterials by sonication. For example, carbon nanotubes are frequently sonicated with PEG-containing surfactants of the Pluronic(®) series or phospholipid-PEG polymers to noncovalently functionalize the nanotubes. However, PEG is very sensitive to degradation upon sonication and the degradation products can be toxic to mammalian cells and to organisms such as zebrafish embryos...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28145974/quantitative-analysis-of-optical-coherence-tomography-angiography-in-adult-onset-foveomacular-vitelliform-dystrophy
#11
Maurizio Battaglia Parodi, Alessandro Rabiolo, Maria Vittoria Cicinelli, Pierluigi Iacono, Francesco Romano, Francesco Bandello
PURPOSE: To quantify the foveal avascular zone area at superficial capillary plexus (SCP) and deep capillary plexus (DCP) and the global, parafoveal, and perifoveal vessel densities at SCP, DCP, and choriocapillaris using optical coherence tomography angiography in patients affected by adult-onset foveomacular vitelliform dystrophy (AOFVD). METHODS: Twenty eyes of 20 consecutive patients (10 females, 50%) with AOFVD and 20 eyes of 20 healthy controls presenting at the Department of Ophthalmology of San Raffaele Hospital, Milan, Italy were enrolled...
January 31, 2017: Retina
https://www.readbyqxmd.com/read/28144754/contact-cooling-of-random-pattern-cutaneous-flaps-does-it-increase-necrosis
#12
Edward Nahabet, Hooman Riazi, Mark Asirwatham, Emily Wirtz, Donald J Harvey, Davood Varghai, Bahman Guyuron
BACKGROUND: Cooling after surgery reduces pain, swelling and ecchymosis. However, the fear of adverse effects of vasoconstriction caused by cooling may prevent its use when the skin is undermined extensively, for example, after rhytidectomy. The purpose of this study is to determine whether the contact cooling of random-pattern skin flaps increases the area of necrosis observed. METHODS: Twenty-eight random-pattern skin flaps (4 × 10 cm) were raised on four pigs...
January 31, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28129915/analysis-of-the-modifications-of-mri-signal-of-the-brachial-plexus-of-rats-comparative-study-before-and-after-freezing-thawing
#13
A Perruisseau-Carrier, N Bahlouli, C Po, P Vernet, S Facca, P Liverneaux
The aim of this study was to compare the MRI signal of the brachial plexus and surrounding muscles before and after freezing/thawing on a murine model. A first MRI going through the brachial plexuses of 5 healthy Wistar rats was performed immediately post-mortem. A second MRI was performed after freezing at -30°C and then thawing at 20°C for 24hours. All MRI images were segmented to make nerve and muscular structures appear and calculate the average intensity of the MRI signal using the program ImageJ. The average nerve and muscular MRI signals were compared before and after freezing/thawing and rated in grayscale units between 0 and 255...
January 24, 2017: Annales de Chirurgie Plastique et Esthétique
https://www.readbyqxmd.com/read/28125294/effects-of-exposure-parameters-and-slice-thickness-on-detecting-clear-and-unclear-mandibular-canals-using-cone-beam-ct
#14
Gainer R Jasa, Mayumi Shimizu, Kazutoshi Okamura, Kenji Tokumori, Yohei Takeshita, Warangkana Weerawanich, Kazunori Yoshiura
: Objectives The purpose of this study was to clarify the effects of exposure parameters and image processing methods when using cone beam CT (CBCT) to detect clear and unclear mandibular canals (MCs). Methods Twenty-four dry half mandibles were divided into two groups with clear and unclear MCs based on a previous CBCT study. Mandibles were scanned using a CBCT system with varying exposure parameters (tube voltages 60, 70, and 90 kV; and tube currents 2, 5, 10 and 15 mA) to obtain a total of 144 scans...
January 26, 2017: Dento Maxillo Facial Radiology
https://www.readbyqxmd.com/read/28110941/assessment-of-the-trabecular-structure-of-mandibular-condyles-in-patients-with-temporomandibular-disorders-using%C3%A2-fractal-analysis
#15
Belde Arsan, Taha Emre Köse, Erhan Çene, İlknur Özcan
OBJECTIVES: The aim of this study was to evaluate changes in the trabecular structure of the mandibular condyle in patients with temporomandibular disorders (TMDs) using fractal analysis. STUDY DESIGN: A total of 100 patients ages 18 to 73 years were clinically assessed using the Diagnostic Criteria for Temporomandibular Disorders. The control group was age- and sex-matched with the patient group. Panoramic radiographs were obtained using a Kodak 8000 digital device with 73 kVp and 5 mA fixed parameters...
March 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28107406/classification-and-segmentation-of-nanoparticle-diffusion-trajectories-in-cellular-micro-environments
#16
Thorsten Wagner, Alexandra Kroll, Chandrashekara R Haramagatti, Hans-Gerd Lipinski, Martin Wiemann
Darkfield and confocal laser scanning microscopy both allow for a simultaneous observation of live cells and single nanoparticles. Accordingly, a characterization of nanoparticle uptake and intracellular mobility appears possible within living cells. Single particle tracking allows to measure the size of a diffusing particle close to a cell. However, within the more complex system of a cell's cytoplasm normal, confined or anomalous diffusion together with directed motion may occur. In this work we present a method to automatically classify and segment single trajectories into their respective motion types...
2017: PloS One
https://www.readbyqxmd.com/read/28101433/use-of-sonic-tomography-to-detect-and-quantify-wood-decay-in-living-trees
#17
Gregory S Gilbert, Javier O Ballesteros, Cesar A Barrios-Rodriguez, Ernesto F Bonadies, Marjorie L Cedeño-Sánchez, Nohely J Fossatti-Caballero, Mariam M Trejos-Rodríguez, José Moises Pérez-Suñiga, Katharine S Holub-Young, Laura A W Henn, Jennifer B Thompson, Cesar G García-López, Amanda C Romo, Daniel C Johnston, Pablo P Barrick, Fulvia A Jordan, Shiran Hershcovich, Natalie Russo, Juan David Sánchez, Juan Pablo Fábrega, Raleigh Lumpkin, Hunter A McWilliams, Kathleen N Chester, Alana C Burgos, E Beatriz Wong, Jonathan H Diab, Sonia A Renteria, Jennifer T Harrower, Douglas A Hooton, Travis C Glenn, Brant C Faircloth, Stephen P Hubbell
PREMISE OF THE STUDY: Field methodology and image analysis protocols using acoustic tomography were developed and evaluated as a tool to estimate the amount of internal decay and damage of living trees, with special attention to tropical rainforest trees with irregular trunk shapes. METHODS AND RESULTS: Living trunks of a diversity of tree species in tropical rainforests in the Republic of Panama were scanned using an Argus Electronic PiCUS 3 Sonic Tomograph and evaluated for the amount and patterns of internal decay...
December 2016: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28098418/investigation-of-the-variability-of-anterior-chamber-scan-protocol-with-cirrus-high-definition-optical-coherence-tomography
#18
Tin A Tun, Shayne S Tan, Eray Atalay, Sushma Verma, Monisha E Nongpiur, Mani Baskaran, Tin Aung, Rahat Husain
IMPORTANCE: The evaluation of anterior chamber scan (ACS) of Cirrus optical coherence tomography (OCT) for routine clinical use BACKGROUND: To assess the variability of anterior chamber angle (ACA) measurements using the ACS DESIGN: Cross-sectional observational study PARTICIPANTS: Forty subjects aged 40-80 years METHODS: One randomly selected eye from 40 subjects was imaged with Cirrus OCT (model 5000, Carl Zeiss Meditec, Dublin, CA) by two different operators (expert vs non-expert) with a 15-minute interval for inter- and intra-observer variability of image acquisition...
January 18, 2017: Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/28095845/relationship-of-diagnostic-accuracy-of-renal-cortical-echogenicity-with-renal-histopathology-in-dogs-and-cats-a-quantitative-study
#19
Tommaso Banzato, Federico Bonsembiante, Luca Aresu, Alessandro Zotti
BACKGROUND: Renal cortical echogenicity is routinely evaluated during ultrasonographic investigation of the kidneys. Both in dog and cat previous ex-vivo studies have revealed a poor correlation between renal echogenicity and corresponding lesions. The aim of this study was to establish the in-vivo relationship between renal cortical echogenicity and renal histopathology. RESULTS: Thirty-eight dogs and fifteen cats euthanized for critical medical conditions were included in the study...
January 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28095484/experimental-diabetes-alters-the-morphology-and-nano-structure-of-the-achilles-tendon
#20
Rodrigo Ribeiro de Oliveira, Rômulo Medina de Mattos, Luciana Magalhães Rebelo, Fernanda Guimarães Meireles Ferreira, Fernanda Tovar-Moll, Luiz Eurico Nasciutti, Gerly Anne de Castro Brito
Although of several studies that associate chronic hyperglycemia with tendinopathy, the connection between morphometric changes as witnessed by magnetic resonance (MR) images, nanostructural changes, and inflammatory markers have not yet been fully established. Therefore, the present study has as a hypothesis that the Achilles tendons of rats with diabetes mellitus (DM) exhibit structural changes. The animals were randomly divided into two experimental groups: Control Group (n = 06) injected with a vehicle (sodium citrate buffer solution) and Diabetic Group (n = 06) consisting of rats submitted to intraperitoneal administration of streptozotocin...
2017: PloS One
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