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https://www.readbyqxmd.com/read/28434682/a-proposed-linear-skeletal-distance-to-predict-occlusal-vertical-dimension-a-cephalometric-study
#1
Mohammed Nasser Alhajj, Ammar A Daer
STATEMENT OF PROBLEM: Establishing a correct occlusal vertical dimension is considered one of the most important aspects of facial esthetics for patients in need of oral rehabilitation. PURPOSE: The purpose of this retrospective study was to evaluate the use of a proposed linear skeletal distance to predict the appropriate occlusal vertical dimension. MATERIAL AND METHODS: A total of 93 digital cephalometric radiographs were collected in Joint Photographic Experts Group (JPEG) format (51 men, 42 women) from the archive of the orthodontics department at the Faculty of Dentistry, Sana'a University, Yemen...
April 20, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28432829/trabecular-mapping-leveraging-geometric-morphometrics-for-analyses-of-trabecular-structure
#2
Adam D Sylvester, Claire E Terhune
OBJECTIVES: Trabecular microstructure of limb bone epiphyses has been used to elucidate the relationship between skeletal form and behavior among mammals. Such studies have often relied on the analysis of a single volume of interest (VOI). Here we present a method for evaluating variation in bone microstructure across articular surfaces by leveraging sliding semilandmarks. METHODS: Two samples were used to demonstrate the proposed methodology and test the hypothesis that microstructural variables are homogeneously distributed: tali from two ape genera (Pan and Pongo, n = 9) and modern human distal femora (n = 10)...
April 22, 2017: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/28429640/spinal-alterations-of-reil-insula-in-alzheimer-s-disease
#3
Foivos E Petrides, Ioannis A Mavroudis, Martha Spilioti, Fotios G Chatzinikolaou, Vasiliki G Costa, Stavros J Baloyannis
Alzheimer's disease (AD) is a progressive neurodegenerative disease that involves numerous cellular and biochemical mechanisms resulting in synaptic alterations and extensive neuronal loss. It is primarily characterized by impairment of memory, associated frequently with mood disorders. Continuous studies have shown that insula may be an important target of AD, but neuropathological alterations have not been described extensively. In the present study, we attempted to describe the morphometric and morphological changes of the spines of Reil insula in AD in comparison with normal aging using a silver impregnation technique...
January 1, 2017: American Journal of Alzheimer's Disease and Other Dementias
https://www.readbyqxmd.com/read/28425932/validation-of-a-novel-technique-and-evaluation-of-the-surface-free-energy-of-food
#4
Tugce Senturk Parreidt, Markus Schmid, Carolin Hauser
Characterizing the physical properties of a surface is largely dependent on determining the contact angle exhibited by a liquid. Contact angles on the surfaces of rough and irregularly-shaped food samples are difficult to measure using a contact angle meter (goniometer). As a consequence, values for the surface energy and its components can be mismeasured. The aim of this work was to use a novel contact angle measurement method, namely the snake-based ImageJ program, to accurately measure the contact angles of rough and irregular shapes, such as food samples, and so enable more accurate calculation of the surface energy of food materials...
April 20, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28423830/bridging-the-semantic-gap-between-diagnostic-histopathology-and-image-analysis
#5
Lamine Traore, Yannick Kergosien, Daniel Racoceanu
With the wider acceptance of Whole Slide Images (WSI) in histopathology domain, automatic image analysis algorithms represent a very promising solution to support pathologist's laborious tasks during the diagnosis process, to create a quantification-based second opinion and to enhance inter-observer agreement. In this context, reference vocabularies and formalization of the associated knowledge are especially needed to annotate histopathology images with labels complying with semantic standards. In this work, we elaborate a sustainable triptych able to bridge the gap between pathologists and image analysis scientists...
2017: Studies in Health Technology and Informatics
https://www.readbyqxmd.com/read/28423698/parameters-of-simultaneous-18f-fdg-pet-mri-predict-tumor-stage-and-several-histopathological-features-in-uterine-cervical-cancer
#6
Alexey Surov, Hans Jonas Meyer, Stefan Schob, Anne-Kathrin Höhn, Kristina Bremicker, Marc Exner, Patrick Stumpp, Sandra Purz
The purpose of this study was to analyze associations between apparent diffusion coefficient (ADC) and standardized uptake values (SUV) values and different histopathological parameters in uterine cervical cancer. 21 patients with primary uterine cervical cancer were involved into the study. All patients underwent a whole body simultaneous18F-FDG PET/MRI. Mean and maximum SUV were noted (SUVmean and SUVmax). In all tumors minimal, mean, and maximal ADC values (ADCmin, ADCmean, and ADCmax) were estimated. Combined parameters were calculated: SUVmax/SUVmean, ADCmin/ ADCmean, SUVmax/ADCmin and SUVmax/ADCmean...
March 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28416437/the-ct-based-fractal-analysis-of-trabecular-bone-structure-may-help-in-detecting-decreased-quality-of-bone-prior-to-urgent-spinal-procedures
#7
Marcin Czyz, Arion Kapinas, James Holton, Renata Pyzik, Bronek M Boszczyk, Nasir A Quraishi
BACKGROUND CONTEXT: To date no reliable method is available to determine the parameters of bone density based on the routine spinal CT in the emergency setup. We propose the use of fractal analysis to detect patients with poor quality of bone prior to urgent or semi-urgent spinal procedures. PURPOSE: To validate the hypothesis that the CT-based fractal analysis of the trabecular bone structure may help in detecting patients with poor quality of bone prior to urgent spinal procedures...
April 14, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28413719/intra-specific-variation-and-allometry-of-the-skull-of-late-cretaceous-side-necked-turtle-bauruemys-elegans-pleurodira-podocnemididae-and-how-to-deal-with-morphometric-data-in-fossil-vertebrates
#8
Thiago F Mariani, Pedro S R Romano
BACKGROUND: Previous quantitative studies on Bauruemys elegans (Suárez, 1969) shell variation, as well as the taphonomic interpretation of its type locality, have suggested that all specimens collected in this locality may have belonged to the same population. We rely on this hypothesis in a morphometric study of the skull. Also, we tentatively assessed the eating preference habits differentiation that might be explained as due to ontogenetic changes. METHODS: We carried out an ANOVA testing 29 linear measurements from 21 skulls of B...
2017: PeerJ
https://www.readbyqxmd.com/read/28406495/quantitative-3d-structured-illumination-microscopy-of-nuclear-structures
#9
Felix Kraus, Ezequiel Miron, Justin Demmerle, Tsotne Chitiashvili, Alexei Budco, Quentin Alle, Atsushi Matsuda, Heinrich Leonhardt, Lothar Schermelleh, Yolanda Markaki
3D structured illumination microscopy (3D-SIM) is the super-resolution technique of choice for multicolor volumetric imaging. Here we provide a validated sample preparation protocol for labeling nuclei of cultured mammalian cells, image acquisition and registration practices, and downstream image analysis of nuclear structures and epigenetic marks. Using immunostaining and replication labeling combined with image segmentation, centroid mapping and nearest-neighbor analyses in open-source environments, 3D maps of nuclear structures are analyzed in individual cells and normalized to fluorescence standards on the nanometer scale...
May 2017: Nature Protocols
https://www.readbyqxmd.com/read/28403808/imagej-macros-for-the-user-friendly-analysis-of-soft-agar-and-wound-healing-assays
#10
João Paulo Silva Nunes, Adriana Abalen Martins Dias
Recent advances in biological imaging techniques and the enormous amount of data they generate call for the development of computational tools for efficient and reliable high-throughput analysis. Several software applications with this functionality are available, and one of the most commonly used is ImageJ. Here, we present two independent macros (WH_NJ and SA_NJ) for automating and facilitating the analysis of images acquired from two in vitro assays frequently used in cancer studies and drug screening: the wound-healing and soft-agar assays...
April 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28403025/p16ink4a-cytokeratin-7-and-ki-67-as-potential-markers-for-low-grade-cervical-intraepithelial-neoplasia-progression
#11
Larissa Bastos Eloy da Costa, Renata De Marchi Triglia, Liliana A Lucci De Angelo Andrade
OBJECTIVE: The aim of this study was to evaluate p16, cytokeratin 7 (CK7), and Ki-67 immunoexpressions in low-grade squamous intraepithelial lesion (LSIL), looking for differences among cases that progress to high-grade squamous intraepithelial lesion, maintain LSIL, or regress. MATERIALS AND METHODS: Sixty-six LSIL biopsies were studied. In the follow-up, a second biopsy showed 28.7% regression, 37.9% LSIL, and 33.4% progressed to high-grade squamous intraepithelial lesion...
April 11, 2017: Journal of Lower Genital Tract Disease
https://www.readbyqxmd.com/read/28395715/-subcellular-re-distribution-of-f-actin-in-mcf7-cells-upon-inducible-expression-of-cdkn2a-p16-ink4a
#12
Jianqing Liang, Manna Wang, Zhengrong Zhang, Long Yuan, You Zheng, Hongyan Huang, Qiang Sun, Jufang Wang, Xiaoning Wang
Objective To investigate the effect of CDKN2A/p16(INK4a), a cyclin-dependent kinase inhibitor, on cell morphology and cytoskeleton in MCF7 breast cancer cells by tetracycline operon (Tet-on) inducible gene expression system. Methods MCF7 cells, genetically null for p16(INK4a) expression, were sequentially infected with two viruses of Tet-on systems to make MCF7-pTet-on-p16(INK4a) cells, which were induced to express p16NK4a by doxycycline (Dox) treatment. Cell growth was evaluated by cell counting. To observe the effect of p16(INK4a) on cell adhesion and cytoskeleton, intercellular adhesion and intracellular F-actin were examined by fluorescent staining with anti-E-cadherin antibody and phalloidin, respectively...
April 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28393458/increased-filaggrin-metabolizing-enzyme-activity-in-atopic-skin-a-pilot-study-using-a-canine-model-of-atopic-dermatitis
#13
Natalia Fanton, Domenico Santoro, Luisa Cornegliani, Rosanna Marsella
BACKGROUND: Filaggrin (FLG) and its metabolites are essential for skin barrier function and hydration of the stratum corneum. Alteration of the FLG metabolism could be the basis for an abnormal skin barrier in allergic dogs. OBJECTIVES: To investigate the expression and distribution of calpain-1, caspase-14, furin and matriptase, four enzymes involved in FLG metabolism, in the skin of atopic and healthy beagles. METHODS: Skin biopsies were collected from four healthy and four atopic beagles before and after allergen exposure...
April 9, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28390286/ij-opencv-combining-imagej-and-opencv-for-processing-images-in-biomedicine
#14
César Domínguez, Jónathan Heras, Vico Pascual
BACKGROUND AND OBJECTIVE: The effective processing of biomedical images usually requires the interoperability of diverse software tools that have different aims but are complementary. The goal of this work is to develop a bridge to connect two of those tools: ImageJ, a program for image analysis in life sciences, and OpenCV, a computer vision and machine learning library. METHODS: Based on a thorough analysis of ImageJ and OpenCV, we detected the features of these systems that could be enhanced, and developed a library to combine both tools, taking advantage of the strengths of each system...
April 1, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28389953/tracking-the-movement-of-a-single-prokaryotic-cell-in-extreme-environmental-conditions
#15
Masayoshi Nishiyama, Yoshiyuki Arai
Many bacterial species move toward favorable habitats. The flagellum is one of the most important machines required for the motility in solution and is conserved across a wide range of bacteria. The motility machinery is thought to function efficiently with a similar mechanism in a variety of environmental conditions, as many cells with similar machineries have been isolated from harsh environments. To understand the common mechanism and its diversity, microscopic examination of bacterial movements is a crucial step...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28385302/subclassification-of-primary-angle-closure-using-anterior-segment-optical-coherence-tomography-and-ultrasound-biomicroscopic-parameters
#16
Junki Kwon, Kyung Rim Sung, Seungbong Han, Ye Ji Moon, Joong Won Shin
PURPOSE: To classify eyes with primary angle closure (PAC) in terms of the features visualized using anterior segment optical coherence tomography (AS-OCT) and ultrasound biomicroscopy (UBM). DESIGN: Retrospective, observational study. PARTICIPANTS: A total of 73 eyes of 73 patients with PAC. METHODS: Participants' eyes that had undergone laser peripheral iridotomy (LPI) were imaged using AS-OCT and UBM under the same lighting conditions...
April 3, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28377323/brain-injury-results-in-lower-levels-of-melatonin-receptors-subtypes-mt1-and-mt2
#17
Nicole D Osier, Lan Pham, Bunny J Pugh, Ava Puccio, Dianxu Ren, Yvette P Conley, Sheila Alexander, C Edward Dixon
BACKGROUND: Traumatic brain injury (TBI) is a devastating and costly acquired condition that affects individuals of all ages, races, and geographies via a number of mechanisms. The effects of TBI on melatonin receptors remain unknown. PURPOSE: The purpose of this study is to explore whether endogenous changes in two melatonin receptor subtypes (MT1 and MT2) occur after experimental TBI. SAMPLE: A total of 25 adult male Sprague Dawley rats were used with 6 or 7 rats per group...
April 2, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28374260/is-there-a-role-for-near-infrared-technology-in-laparoscopic-resection-of-pancreatic-neuroendocrine-tumors-results-of-the-colpan-colour-and-resect-the-pancreas-study
#18
Salvatore Paiella, Matteo De Pastena, Luca Landoni, Alessandro Esposito, Luca Casetti, Marco Miotto, Marco Ramera, Roberto Salvia, Erica Secchettin, Deborah Bonamini, Gessica Manzini, Mirko D'Onofrio, Giovanni Marchegiani, Claudio Bassi
BACKGROUND: The intraoperative identification of pancreatic neuroendocrine tumors (PanNETs) is of utmost importance to drive their laparoscopic resection. Near-infrared (NIR) surgery has emerged as a new technique for localizing tumors or neoplastic tissue. This study aimed to explore the results of the application of NIR in the laparoscopic resection of PanNETs. METHODS: Per protocol we enrolled ten subjects undergoing laparoscopic pancreatic surgery for PanNET from March 2016 to October 2016...
April 3, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28374129/dot-immunoassay-for-the-simultaneous-determination-of-postvaccination-immunity-against-pertussis-diphtheria-and-tetanus
#19
Pavel Khramtsov, Maria Bochkova, Valeria Timganova, Svetlana Zamorina, Mikhail Rayev
A dot immunoassay for simultaneous semiquantitative detection of IgG against tetanus toxoid (Ttx) and diphtheria toxoid (Dtx) and qualitative detection of anti-Bordetella pertussis IgGs in human blood serum using carbon nanoparticles functionalized with streptococcal protein G was developed. Inactivated B. pertussis cells in suspension form were used as an antigen in the immunoassay. Pertussis, tetanus, and diphtheria antigens were separately spotted onto nitrocellulose strips, and then the immunostrips were successively incubated with blood sera and a suspension of carbon nanoparticles...
April 3, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28373429/dna-double-strand-breaks-induced-byfractionated-neutron-beam-irradiation-for-boron-neutron-capture-therapy
#20
Yuko Kinashi, Natsuya Yokomizo, Sentaro Takahashi
AIM: To use the 53BP1 foci assay to detect DNA double-strand breaks induced by fractionated neutron beam irradiation of normal cells. MATERIALS AND METHODS: The Kyoto University Research Reactor heavy-water facility and gamma-ray irradiation system were used as experimental radiation sources. After fixation of Chinese Hamster Ovary cells with 3.6% formalin, immunofluorescence staining was performed. Number and size of foci were analyzed using ImageJ software. RESULTS: Fractionated neutron irradiation induced 25% fewer 53BP1 foci than single irradiation at the same dose...
April 2017: Anticancer Research
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