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https://www.readbyqxmd.com/read/28342044/3d-histopathology-a-lung-tissue-segmentation-workflow-for-microfocus-x-ray-computed-tomography-scans
#1
Lasse Wollatz, Steven J Johnston, Peter M Lackie, Simon J Cox
Lung histopathology is currently based on the analysis of 2D sections of tissue samples. The use of microfocus X-ray-computed tomography imaging of unstained soft tissue can provide high-resolution 3D image datasets in the range of 2-10 μm without affecting the current diagnostic workflow. Important details of structural features such as the tubular networks of airways and blood vessels are contained in these datasets but are difficult and time-consuming to identify by manual image segmentation. Providing 3D structures permits a better understanding of tissue functions and structural interrelationships...
March 24, 2017: Journal of Digital Imaging: the Official Journal of the Society for Computer Applications in Radiology
https://www.readbyqxmd.com/read/28339458/rapid-surveillance-for-vector-presence-rsvp-development-of-a-novel-system-for-detecting-aedes-aegypti-and-aedes-albopictus
#2
Brian L Montgomery, Martin A Shivas, Sonja Hall-Mendelin, Jim Edwards, Nicholas A Hamilton, Cassie C Jansen, Jamie L McMahon, David Warrilow, Andrew F van den Hurk
BACKGROUND: The globally important Zika, dengue and chikungunya viruses are primarily transmitted by the invasive mosquitoes, Aedes aegypti and Aedes albopictus. In Australia, there is an increasing risk that these species may invade highly urbanized regions and trigger outbreaks. We describe the development of a Rapid Surveillance for Vector Presence (RSVP) system to expedite presence- absence surveys for both species. METHODOLOGY/PRINCIPAL FINDINGS: We developed a methodology that uses molecular assays to efficiently screen pooled ovitrap (egg trap) samples for traces of target species ribosomal RNA...
March 24, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28336360/gain-a-graph-based-method-to-automatically-analyze-neurite-outgrowth-in-cell-cultures
#3
B L Long, H Li, A Mahadevan, T Tang, K Balotin, N Grandel, J Soto, S Y Wong, A Abrego, S Li, A A Qutub
BACKGROUND: Neurite outgrowth is a metric widely used to assess the success of in vitro neural stem cell differentiation or neuron reprogramming protocols and of high-content screening assays for neural regenerative drug discovery. However, neurite measurements are tedious to perform manually, and there is a paucity of freely available, fully automated software to determine neurite measurements and neuron counting. To provide such a tool to the neurobiology, stem cell, cell engineering, and neuroregenerative communities, we developed an algorithm for performing high-throughput neurite analysis in immunofluorescence images...
March 20, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28332239/clinical-application-of-calculated-split-renal-volume-using-computed-tomography-based-renal-volumetry-after-partial-nephrectomy-correlation-with-technetium-99m-dimercaptosuccinic-acid-renal-scan-data
#4
Chan Ho Lee, Young Joo Park, Ja Yoon Ku, Hong Koo Ha
OBJECTIVE: To evaluate the clinical application of computed tomography-based measurement of renal cortical volume and split renal volume as a single tool to assess the anatomy and renal function in patients with renal tumors before and after partial nephrectomy, and to compare the findings with technetium-99m dimercaptosuccinic acid renal scan. METHODS: The data of 51 patients with a unilateral renal tumor managed by partial nephrectomy were retrospectively analyzed...
March 22, 2017: International Journal of Urology: Official Journal of the Japanese Urological Association
https://www.readbyqxmd.com/read/28314612/a-simple-imagej-macro-tool-for-analyzing-mitochondrial-network-morphology-in-mammalian-cell-culture
#5
Andrew J Valente, Lucas A Maddalena, Ellen L Robb, Fereshteh Moradi, Jeffrey A Stuart
Mitochondria exist in a dynamic cycle of fusion and fission whose balance directly influences the morphology of the 'mitochondrial network', a term that encompasses the branched, reticular structure of fused mitochondria as well as the separate, punctate individual organelles within a eukaryotic cell. Over the past decade, the significance of the mitochondrial network has been increasingly appreciated, motivating the development of various approaches to analyze it. Here, we describe the Mitochondrial Network Analysis (MiNA) toolset, a relatively simple pair of macros making use of existing ImageJ plug-ins, allowing for semi-automated analysis of mitochondrial networks in cultured mammalian cells...
March 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28302946/influence-of-lyophilization-factors-and-gelatin-concentration-on-pore-structures-of-atelocollagen-gelatin-sponge-biomaterial
#6
Longqiang Yang, Koji Tanabe, Tadashi Miura, Masao Yoshinari, Shinji Takemoto, Seikou Shintani, Masataka Kasahara
This study aimed to investigate influences of lyophilization factors and gelatin concentration on pore structures of ACG sponge. ACG sponges of different freezing temperatures (-30, -80 and -196(o)C), freezing times (1, 2 and 24 h), gelatin concentrations (0.6%AC+0.15%G, 0.6%AC+0.6%G and 0.6%AC+2.4%G), and with 500 μM fluvastatin were fabricated. Pore structures including porosity and pore size were analyzed by scanning electron microscopy and ImageJ. The cytotoxic effects of ACG sponges were evaluated in vitro...
March 8, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28301022/use-of-an-alternate-light-source-to-detect-tooth-and-bone
#7
Geraldo Elias Miranda, Rodolfo Francisco Haltenhoff Melani, Luiz Francisquini, Eduardo Daruge
The aim of this study was to identify the combination of wavelength and filter that best detects tooth and bone, and to determine which biological materials (enamel, dental root or bone) have highest fluorescence intensity when exposed to an alternate light source (ALS). Tooth and bone samples were lighted with ALS and photographed. Adobe Photoshop™ and ImageJ™ softwares were used for image analysis. Data obtained by measuring the photograph pixels were subjected to analysis of variance. The mean values of significant effects were compared by the Tukey test...
January 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/28298832/reconstruction-of-interdental-papilla-using-autogenous-bone-and-connective-tissue-grafts
#8
Santhanakrishnan Muthukumar, Pooja Ajit, Shiyamali Sundararajan, Suresh Ranga Rao
Previous studies have reported the management of Class I and II papillary defects, but knowledge on Class III defects, estimated to have a poor periodontal prognosis, remains minimal. In this case report, a Class III papillary defect reconstruction was attempted mainly since the patient reported with difficulty in phonetics. In Stage I, autogenous bone graft from the maxillary tuberosity and subepithelial connective tissue graft was augmented to decrease the distance between the interdental bone crest and contact point, simultaneously achieving a switch in the periodontal biotype...
July 2016: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/28296321/measurement-of-image-rotation-angle-in-ct-for-radiotherapy-treatment-planning
#9
Witold Skrzynski, Wioletta Ślusarczyk-Kacprzyk
The geometric accuracy of CT images is essential when they are used for planning radiation therapy. In this study, a method of quantitative testing of image rotation in CT is presented, based on the automated analysis of an image of a phantom half-filled with water. A plug-in was written for ImageJ software for automated detection of a water surface in an image and measurement of its angle relative to a horizontal line. A water phantom can be used for the evaluation of image rotation for axial mode. In helical mode the movement of the table would cause movement of the water...
July 2016: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28293873/the-association-between-nd-yag-laser-and-desensitizing-dentifrices-for-the-treatment-of-dentin-hypersensitivity
#10
Sandra Ribeiro Cunha, Stephanie Assimakopoulos Garófalo, Taís Scaramucci, Denise Maria Zezell, Ana Cecilia Corrêa Aranha
This study aims to evaluate the association between Nd:YAG laser (with and without a photoabsorber) and two desensitizing dentifrices containing 15% NovaMin or 8% arginine, as potential treatments for dentin hypersensitivity (DH). DH was simulated by EDTA application for 2 min. Specimens were then analyzed with an environmental scanning electron microscope (ESEM) to ensure open dentin tubules (ODT), counted by using ImageJ software. Specimens were randomized into eight groups (n = 10): Laser (L), Laser+Photoabsorber (LP), Arginine (A), Arginine+Laser (AL), Arginine+Laser+Photoabsorber (ALP), NovaMin (N), NovaMin+Laser (NL), and NovaMin+Laser+Photoabsorber (NLP)...
March 14, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28292256/a-novel-measure-and-significance-testing-in-data-analysis-of-cell-image-segmentation
#11
Jin Chu Wu, Michael Halter, Raghu N Kacker, John T Elliott, Anne L Plant
BACKGROUND: Cell image segmentation (CIS) is an essential part of quantitative imaging of biological cells. Designing a performance measure and conducting significance testing are critical for evaluating and comparing the CIS algorithms for image-based cell assays in cytometry. Many measures and methods have been proposed and implemented to evaluate segmentation methods. However, computing the standard errors (SE) of the measures and their correlation coefficient is not described, and thus the statistical significance of performance differences between CIS algorithms cannot be assessed...
March 14, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28284896/progesterone-increased-%C3%AE-endorphin-innervation-of-the-locus-coeruleus-but-ovarian-steroids-had-no-effect-on-noradrenergic-neurodegeneration
#12
Fernanda B Lima, Cristiane M Leite, Cynthia L Bethea, Janete A Anselmo-Franci
With the decline of ovarian steroids levels at menopause, many women experience an increase in anxiety and stress sensitivity. The locus coeruleus (LC), a central source of noradrenaline (NE), is activated by stress and is inhibited by β-endorphin. Moreover, increased NE has been implicated in pathological anxiety syndromes. Hormone replacement therapy (HRT) in menopause appears to decrease anxiety and vulnerability to stress. Therefore, we questioned the effect of HRT on the inhibitory β-endorphin innervation of the LC...
March 8, 2017: Brain Research
https://www.readbyqxmd.com/read/28272929/perfusion-of-the-rotator-cuff-tendon-according-to-the-repair-configuration-using-an-indocyanine-green-fluorescence-arthroscope
#13
Sae Hoon Kim, Won-Sang Cho, Ho-Yun Joung, Young Eun Choi, Minwoong Jung
BACKGROUND: The disturbance of rotator cuff tendon perfusion has been connected with the suture-bridge configuration repair (SBCR) technique; however, in vivo assessments of the tendon blood supply have been problematic with other modalities. An evaluation of tissue perfusion by an indocyanine green (ICG) fluorescence arthroscope has been developed to counteract this difficulty. PURPOSE: To verify the hindrance of perfusion in SBCR, we used an ICG fluorescence camera to compare parallel-type transosseous repair (PTR) and SBCR in rabbits immediately and at 3 days after rotator cuff repair...
March 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28267872/a-3d-imaging-and-visualization-workflow-using-confocal-microscopy-and-advanced-image-processing-for-brachyuran-crab-larvae
#14
S A Kamanli, T C Kihara, A D Ball, D Morritt, P F Clark
Confocal laser scanning microscopy is an excellent tool for nondestructive imaging of arthropods and can provide detailed information on morphology including fine surface detail. A methodology is presented here for the visualization by confocal microscopy of arthropods, using brachyuran crab zoeal stages as examples and postprocessing techniques derived from micro-CT protocols to improve the final images. This protocol is divided into description of the preprocessing steps (cleaning, staining, digesting and mounting), confocal laser scanning microscopy and data visualization using open-source, freeware programs ImageJ and Drishti...
March 7, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28264244/demineralized-bone-matrix-dbm-as-a-bone-void-filler-in-lumbar-interbody-fusion-a-prospective-pilot-study-of-simultaneous-dbm-and-autologous-bone-grafts
#15
Bum-Joon Kim, Se-Hoon Kim, Haebin Lee, Seung-Hwan Lee, Won-Hyung Kim, Sung-Won Jin
OBJECTIVE: Solid bone fusion is an essential process in spinal stabilization surgery. Recently, as several minimally invasive spinal surgeries have developed, a need of artificial bone substitutes such as demineralized bone matrix (DBM), has arisen. We investigated the in vivo bone growth rate of DBM as a bone void filler compared to a local autologous bone grafts. METHODS: From April 2014 to August 2015, 20 patients with a one or two-level spinal stenosis were included...
March 2017: Journal of Korean Neurosurgical Society
https://www.readbyqxmd.com/read/28258811/human-and-swine-dental-pulp-stem-cells-form-a-vascularlike-network-after-angiogenic-differentiation-in-comparison-with-endothelial-cells-a-quantitative-analysis
#16
Hacer Aksel, George T-J Huang
INTRODUCTION: The aim of this study was to quantify vascular network formation capacity after angiogenic induction of human and swine dental pulp stem cells (DPSCs) in comparison with endothelial cells. METHODS: Primary human DPSCs or swine DPSCs were induced in endothelial growth medium for 7 days. The expression of the endothelial marker von Willebrand factor was determined by immunostaining. Induced DPSCs (iDPSCs) and noninduced DPSCs (niDPSCs) were seeded at different cell numbers onto Matrigel (BD Biosciences, San Jose, CA) for vascular network formation assays and analyzed after 4, 8, 12, and 18 hours in comparison with human microvascular endothelial cells (hMECs)...
April 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28256485/evaluation-of-various-filling-techniques-in-distal-canals-of-mandibular-molars-instrumented-with-different-single-file-nickel-titanium-systems
#17
A Dumani, S Yilmaz, O Yoldas, C Kuden
BACKGROUND AND AIMS: The aim of this study was to evaluate the quality of various filling techniques in distal canals of mandibular molars instrumented with different single-file nickel-titanium (NiTi) systems. MATERIALS AND METHODS: A total of 150 distal roots of mandibular molar teeth were randomly assigned into three main groups and instrumented by using Reciproc (VDW, Munich, Germany), WaveOne (Dentsply Tulsa, Tulsa, OK, USA), or One Shape (MicroMega, Besancon, France) NiTi file systems...
March 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28243788/quantifying-filopodia-in-cultured-astrocytes-by-an-algorithm
#18
Georg Aumann, Felix Friedländer, Matthias Thümmler, Fabian Keil, Robert Brunkhorst, Horst-Werner Korf, Amin Derouiche
Astrocytes in vivo extend thin processes termed peripheral astrocyte processes (PAPs), in particular around synapses where they can mediate glia-neuronal communication. The relation of PAPs to synapses is not based on coincidence, but it is not clear which stimuli and mechanisms lead to their formation and are active during process extension/ retraction in response to neuronal activity. Also, the molecular basis of the extremely fine PAP morphology (often 50 to 100 nm) is not understood. These open questions can be best investigated under in vitro conditions studying glial filopodia...
February 27, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28243706/bone-texture-analysis-using-ct-simulation-scans-to-individuate-risk-parameters-for-radiation-induced-insufficiency-fractures
#19
V Nardone, P Tini, S F Carbone, A Grassi, M Biondi, L Sebaste, T Carfagno, E Vanzi, G De Otto, G Battaglia, G Rubino, P Pastina, G Belmonte, L N Mazzoni, F Banci Buonamici, M A Mazzei, L Pirtoli
This study deals with the role of texture analysis as a predictive factor of radiation-induced insufficiency fractures in patients undergoing pelvic radiation. INTRODUCTION: This study aims to assess the texture analysis (TA) of computed tomography (CT) simulation scans as a predictive factor of insufficiency fractures (IFs) in patients with pelvic malignancies undergoing radiation therapy (RT). METHODS: We performed an analysis of patients undergoing pelvic RT from January 2010 to December 2014, 24 of whom had developed pelvic bone IFs...
February 27, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28238558/a-novel-computer-system-for-the-evaluation-of-nasolabial-morphology-symmetry-and-aesthetics-after-cleft-lip-and-palate-treatment-part-1-general-concept-and-validation
#20
Piotr Pietruski, Marcin Majak, Tomasz Debski, Boguslaw Antoszewski
PURPOSE: The need for a widely accepted method suitable for a multicentre quantitative evaluation of facial aesthetics after surgical treatment of cleft lip and palate (CLP) has been emphasized for years. The aim of this study was to validate a novel computer system 'Analyse It Doc' (A.I.D.) as a tool for objective anthropometric analysis of the nasolabial region. MATERIALS AND METHODS: An indirect anthropometric analysis of facial photographs was conducted with the A...
February 1, 2017: Journal of Cranio-maxillo-facial Surgery
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