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https://www.readbyqxmd.com/read/29330515/hyphatracker-an-imagej-toolbox-for-time-resolved-analysis-of-spore-germination-in-filamentous-fungi
#1
Michael Brunk, Sebastian Sputh, Sören Doose, Sebastian van de Linde, Ulrich Terpitz
The dynamics of early fungal development and its interference with physiological signals and environmental factors is yet poorly understood. Especially computational analysis tools for the evaluation of the process of early spore germination and germ tube formation are still lacking. For the time-resolved analysis of conidia germination of the filamentous ascomycete Fusarium fujikuroi we developed a straightforward toolbox implemented in ImageJ. It allows for processing of microscopic acquisitions (movies) of conidial germination starting with drift correction and data reduction prior to germling analysis...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29329121/technique-for-preparing-ultrathin-and-nanothin-descemet-stripping-automated-endothelial-keratoplasty-tissue
#2
Albert Y Cheung, Joshua H Hou, Peter Bedard, Veronique Grimes, Natalie Buckman, Medi Eslani, Edward J Holland
PURPOSE: To describe and report outcomes of our single-pass microkeratome technique for preparation of ultrathin (UT, ≤100 μm) and nanothin (NT, ≤50 μm) Descemet stripping automated endothelial keratoplasty (DSAEK) grafts. METHODS: To prepare NT-DSAEK grafts, a pachymetry nomogram specific to each technician and individual microkeratome head was developed based on accumulated precut and postcut pachymetry data from previous DSAEK grafts. Mean graft thickness as well as precut and postcut endothelial cell counts (ECCs) of NT-DSAEK, UT-DSAEK, and Descemet membrane endothelial keratoplasty (DMEK) grafts between July 2015 and July 2017 were calculated and compared statistically...
January 11, 2018: Cornea
https://www.readbyqxmd.com/read/29323728/3d-reconstruction-of-prostate-cancer-architecture-with-serial-immunohistochemical-sections-hallmarks-of-tumour-growth-tumour-compartmentalization-and-implications-for-grading-and-heterogeneity
#3
Yuri Tolkach, Stefan Thomann, Glen Kristiansen
BACKGROUND: Conventional morphology of the prostate cancer (PCA) considers only two-dimensional architecture of the tumour. Our aim was to examine the feasibility of 3D-reconstruction of tumour morphology based on multiple consecutive histological sections and to decipher relevant features of PCA architecture. METHODS: Seventy-five consecutive histological sections (5μm) of a typical prostate adenocarcinoma (Gleason-Score 3+4=7) were immunostained (Pan-Cytokeratin) and scanned for further 3D reconstructions with Fiji / ImageJ software...
January 11, 2018: Histopathology
https://www.readbyqxmd.com/read/29321450/microfluidic-paper-based-analytical-devices-for-determination-of-creatinine-in-urine-samples
#4
Suphanan Sununta, Poomrat Rattanarat, Orawon Chailapakul, Narong Praphairaksit
Simple, low-cost and portable microfluidic paper-based analytical devices (μPADs) for determination of creatinine in urine samples were developed. The methodology was based on Jaffé reaction between the creatinine and picric acid in alkaline conditions, generating a colorimetric creatinine-alkaline picrate complex. The product exhibits an orange color that is clearly visible on the μPADs. The color intensity of the complex, which is indicative of the concentration of creatinine, is then quantitatively determined using ImageJ software...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29319936/submucosal-gland-mucus-strand-velocity-is-decreased-in-chronic-rhinosinusitis
#5
Kiranya E Tipirneni, Shaoyan Zhang, Do-Yeon Cho, Jessica Grayson, Daniel F Skinner, Calvin Mackey, Lindsay Moore, Denzel Cole, Catherine G Banks, Bradford A Woodworth
BACKGROUND: Chronic rhinosinusitis (CRS) may be initiated by innately impaired host defense mechanisms that predispose the upper airways to infection. Recent evidence suggests tethering of submucosal gland mucus strands represents an inciting event within cystic fibrosis (CF) airways, occurring prior to onset of chronic infection. Submucosal gland hypertrophy and defective mucociliary clearance (MCC) are present in actively inflamed sinuses, but mucus strand velocity may also be affected as a secondary event, further contributing to chronic disease...
January 10, 2018: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/29319229/-cyt-nuc-a-customizable-and-documenting-imagej-macro-for-evaluation-of-protein-distributions-between-cytosol-and-nucleus
#6
Tilman Grune, Richard Kehm, Annika Höhn, Tobias Jung
Large amounts of data from multi-channel, high resolution, fluorescence microscopic images require tools that provide easy, customizable and reproducible high-throughput analysis. The freeware "ImageJ" has become one of the standard tools for scientific image analysis. Since ImageJ offers recording of "macros", even a complex multi-step process can be easily applied fully automated to large numbers of images, saving both time and reducing human subjective evaluation. In this work, we present "Cyt/Nuc", an ImageJ macro, able to recognize and to compare the nuclear and cytosolic areas of tissue samples, in order to investigate distributions of immunostained proteins between both compartments, while it documents in detail the whole process of evaluation and pattern recognition...
January 10, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29318552/beyond-the-proteolytic-activity-examining-the-functional-relevance-of-the%C3%A2-ancillary-domains-using-tri-dimensional-3d-spheroid-invasion-assay
#7
Erico Tosoni Costa, Anamaria Aranha Camargo
In this chapter, we describe a straightforward protocol to generate multicellular tumor spheroids (MTSs) and evaluate the role of specific genes in regulating cell invasiveness in real-time and tridimensional (3D) matrices. This approach provides advantages over other conventional invasion assays by offering intimate cell-cell and cell-ECM contacts and by mimicking the pathophysiological characteristics observed in tumor microenvironments (e.g., microregional gradients in glucose and O2 concentrations and metabolic and proliferative tumor heterogeneity)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29304059/space-use-by-4-strains-of-laying-hens-to-perch-wing-flap-dust-bathe-stand-and-lie-down
#8
Elizabeth R Riddle, Ahmed B A Ali, Dana L M Campbell, Janice M Siegford
The laying hen industry is implementing aviary systems intended to improve welfare by providing hens with more space and resources to perform species-specific behaviors. To date, limited research has examined spatial requirements of various strains of laying hens for performing key behaviors and none has been conducted within an alternative housing system. This study investigated the amount of space used by 4 strains of laying hens (Hy-Line Brown [HB], Bovans Brown [BB], DeKalb White [DW], and Hy-Line W36) to perform 5 different behaviors in the litter area of a commercial-style aviary...
2018: PloS One
https://www.readbyqxmd.com/read/29302731/quantifying-dynamic-deformity-after-dual-plane-breast-augmentation
#9
Marcelo Recondo Cheffe, Jorge Diego Valentini, Marcus Vinicius Martins Collares, Pedro Salomão Piccinini, Jefferson Luis Braga da Silva
BACKGROUND: Dynamic breast deformity (DBD) is characterized by visible distortion and deformity of the breast due to contraction of the pectoralis major muscle after submuscular breast augmentation; fortunately, in most cases, this is not a clinically significant complaint from patients. The purpose of this study is to present a simple method for objectively measuring DBD in patients submitted to dual plane breast augmentation (DPBA). METHODS: We studied 32 women, between 18 and 50 years old, who underwent primary DPBA with at least 1 year of follow-up...
January 4, 2018: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/29298687/segmentation-error-in-spectral-domain-optical-coherence-tomography-measures-of-the-retinal-nerve-fibre-layer-thickness-in-idiopathic-intracranial-hypertension
#10
Anuriti Aojula, Susan P Mollan, John Horsburgh, Andreas Yiangou, Kiera A Markey, James L Mitchell, William J Scotton, Pearse A Keane, Alexandra J Sinclair
BACKGROUND: Optical Coherence Tomography (OCT) imaging is being increasingly used in clinical practice for the monitoring of papilloedema. The aim is to characterise the extent and location of the Retinal Nerve Fibre Layer (RNFL) Thickness automated segmentation error (SegE) by manual refinement, in a cohort of Idiopathic Intracranial Hypertension (IIH) patients with papilloedema and compare this to controls. METHODS: Baseline Spectral Domain OCT (SDOCT) scans from patients with IIH, and controls with no retinal or optic nerve pathology, were examined...
January 4, 2018: BMC Ophthalmology
https://www.readbyqxmd.com/read/29288639/retinal-vascular-impairment-in-best-vitelliform-macular-dystrophy-assessed-by-means-of-optical-coherence-tomography-angiography
#11
Maurizio Battaglia Parodi, Francesco Romano, Maria Vittoria Cicinelli, Alessandro Rabiolo, Alessandro Arrigo, Luisa Pierro, Pierluigi Iacono, Francesco Bandello
PURPOSE: To evaluate vascular abnormalities at superficial (SCP), deep (DCP) capillary plexuses and choriocapillaris (CC) in patients with Best vitelliform macular dystrophy (BVMD) by means of optical coherence tomography angiography (OCT-A). DESIGN: Cross-sectional case series. METHODS: Sixty-six eyes of 33 patients with BVMD (sixteen males) and 33 controls were consecutively enrolled. Patients were subdivided in classic stages and underwent best-corrected visual acuity (BCVA), fundus autofluorescence and spectral domain-optical coherence tomography (Spectralis HRA+OCT; Heidelberg Engineering), and 4...
December 27, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29288586/promoij-a-new-tool-for-automatic-three-dimensional-analysis-of-microglial-process-motility
#12
Iñaki Paris, Julie C Savage, Laura Escobar, Oihane Abiega, Steven Gagnon, Chin-Wai Hui, Marie-Ève Tremblay, Amanda Sierra, Jorge Valero
Microglia, the immune cells of the central nervous system, continuously survey the brain to detect alterations and maintain tissue homeostasis. The motility of microglial processes is indicative of their surveying capacity in normal and pathological conditions. The gold standard technique to study motility involves the use of two-photon microscopy to obtain time-lapse images from brain slices or the cortex of living animals. This technique generates four dimensionally-coded images which are analyzed manually using time-consuming, non-standardized protocols...
December 30, 2017: Glia
https://www.readbyqxmd.com/read/29284107/assessing-the-appearance-and-fluorescence-of-resin-infiltrated-white-spot-lesions-with-caries-detection-devices
#13
K Markowitz, K Carey
OBJECTIVE: This in vitro study examined the effectiveness of caries detector devices in assessing the ability of resin infiltration (RI) (Icon, DMG-Hamburg, Hamburg, Germany) to improve the optical properties of enamel white spot lesions (WSLs). METHODS AND MATERIALS: Ten caries-free third molars were used. Photographs, a subjective visual assessment of the photographs, fluorescent camera (FC) images using the Spectra (Air Techniques, Melville, NY, USA), and laser fluorescent (LF) readings using the DIAGNOdent (KaVo, Biberach, Germany) were obtained from each tooth's buccal surface...
January 2018: Operative Dentistry
https://www.readbyqxmd.com/read/29273231/voice-acoustic-analysis-of-pediatric-vocal-nodule-patients-using-ratios-calculated-with-biomedical-image-segmentation
#14
Nagihan Bilal, Turab Selcuk, Selman Sarica, Ahmet Alkan, İsrafil Orhan, Adem Doganer, Saime Sagiroglu, Mehmet Akif Kılıc
OBJECTIVE: The aim of this study was to determine nodules using newly developed software with a computer-assisted visual process technique for the calculation of size. The effects of the ratios of nodule base and width were evaluated with voice acoustic analysis. METHODS: A total of 72 patients with pediatric vocal nodule were evaluated. Nodules were marked with the ImageJ News program on photographs obtained from the video recordings in the videostroboscopic examination and classified according to the Shah et al scale...
December 19, 2017: Journal of Voice: Official Journal of the Voice Foundation
https://www.readbyqxmd.com/read/29259701/quantitative-assessment-of-angiogenesis-and-pericyte-coverage-in-human-cell-derived-vascular-sprouts
#15
Jan Eglinger, Haiko Karsjens, Eckhard Lammert
Background: Pericytes, surrounding the endothelium, fulfill diverse functions that are crucial for vascular homeostasis. The loss of pericytes is associated with pathologies, such as diabetic retinopathy and Alzheimer's disease. Thus, there exists a need for an experimental system that combines pharmacologic manipulation and quantification of pericyte coverage during sprouting angiogenesis. Here, we describe an in vitro angiogenesis assay that develops lumenized vascular sprouts composed of endothelial cells enveloped by pericytes, with the additional ability to comparatively screen the effect of multiple small molecules simultaneously...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29251541/imagej-a-free-easy-and-reliable-method-to-measure-leg-ulcers-using-digital-pictures
#16
Javier Aragón-Sánchez, Yurena Quintana-Marrero, Cristina Aragón-Hernández, María José Hernández-Herero
Wound measurement to document the healing course of chronic leg ulcers has an important role in the management of these patients. Digital cameras in smartphones are readily available and easy to use, and taking pictures of wounds is becoming a routine in specialized departments. Analyzing digital pictures with appropriate software provides clinicians a quick, clean, and easy-to-use tool for measuring wound area. A set of 25 digital pictures of plain foot and leg ulcers was the basis of this study. Photographs were taken placing a ruler next to the wound in parallel with the healthy skin with the iPhone 6S (Apple Inc, Cupertino, CA), which has a camera of 12 megapixels using the flash...
December 2017: International Journal of Lower Extremity Wounds
https://www.readbyqxmd.com/read/29251450/correspondence-between-fiber-post-and-drill-dimensions-for-post-canal-preparation
#17
Ricardo Pablo Portigliatti, José Luis Tumini, Alejandro Daniel Bertoldi Hepburn, Romina Flavia Aromando, Jorge Lorenzo Olmos
PURPOSE: To compare fiber posts of several calibers and trademarks to their corresponding root canal preparation drills. METHODS: Three widely used endodontic post brands and their drills were evaluated: Exacto, ParaPost Taper Lux, and Macro-Lock Illusion X-RO. Fiber posts and drills were microphotographed with a scanning electron microscope and images were analyzed using ImageJ image processing software. Fiber post diameter on apical extreme (Pd0), fiber post diameter at 5 mm from the apical extreme (Pd5), drill diameter on apical extreme (Dd0) and drill diameter at 5 mm from the apical extreme (Dd5) were analyzed...
December 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29249023/cortical-fractal-analysis-and-collagen-crosslinks-content-in-femoral-neck-after-osteoporotic-fracture-in-postmenopausal-women-comparison-with-osteoarthritis
#18
Gustavo Davi Rabelo, Jean-Paul Roux, Nathalie Portero-Muzy, Evelyne Gineyts, Roland Chapurlat, Pascale Chavassieux
The femoral neck (FN) has been previously characterized by thinner cortices in osteoporotic fracture (HF) when compared to hip osteoarthritis (HOA). The purposes of this study were to complete the previous investigations on FNs from HF and HOA by analyzing the complexity of the cortical structure and to approach the intrinsic properties of cortical bone by assessing the collagen crosslink contents. FN samples were obtained during arthroplasty in 35 postmenopausal women (HF; n = 17; mean age 79 ± 2 years; HOA; n = 18; mean age 66 ± 2 years)...
December 16, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/29246392/image-analysis-of-interarytenoid-area-to-detect-cases-of-laryngopharyngeal-reflux-an-objective-method
#19
Anindya Nayak, Sunil Kumar, Rubeena Arora, Gautam Bir Singh
PURPOSE: To diagnose Laryngopharyngeal Reflux by observing colour (Red, Green, Blue) at the interarytenoid area during 70° laryngeal endoscopy. MATERIALS AND METHODS: Endoscopic images from 50 normal controls and 50 patients of LPR were obtained in this observational study. LPR patients were selected on the basis of RSI and RFS. Images were analysed using ImageJ, a free image analysis software, developed by the National Institute of Health (NIH). Colour changes in the form of RGB (red, green, blue) values were calculated and analysed at the interarytenoid area...
November 28, 2017: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/29240235/three-dimensional-digital-reconstruction-of-skin-epidermis-and-dermis
#20
P Liu, J-Y Zhu, B Tang, Z-C Hu
This study describes how three-dimensional (3D) human skin tissue is reconstructed, and provides digital anatomical data for the physiological structure of human skin tissue based on large-scale thin serial sections. Human skin samples embedded in paraffin were cut serially into thin sections and then stained with hematoxylin-eosin. Images of serial sections obtained from lighting microscopy were scanned and aligned by the scale-invariant feature transform algorithm. 3D reconstruction of the skin tissue was generated using Mimics software...
December 14, 2017: Journal of Microscopy
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