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https://www.readbyqxmd.com/read/29780324/intercalated-disk-extracellular-nanodomain-expansion-in-patients-with-atrial-fibrillation
#1
Tristan B Raisch, Matthew S Yanoff, Timothy R Larsen, Mohammed A Farooqui, D Ryan King, Rengasayee Veeraraghavan, Robert G Gourdie, Joseph W Baker, William S Arnold, Soufian T AlMahameed, Steven Poelzing
Aims: Atrial fibrillation (AF) is the most common sustained arrhythmia. Previous evidence in animal models suggests that the gap junction (GJ) adjacent nanodomain - perinexus - is a site capable of independent intercellular communication via ephaptic transmission. Perinexal expansion is associated with slowed conduction and increased ventricular arrhythmias in animal models, but has not been studied in human tissue. The purpose of this study was to characterize the perinexus in humans and determine if perinexal expansion associates with AF...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29778280/influence-of-cataract-surgery-on-meibomian-gland-dysfunction
#2
A El Ameen, S Majzoub, G Vandermeer, P-J Pisella
PURPOSE: To evaluate the influence of cataract surgery on Meibomian gland dysfunction, in particular on postoperative functional symptoms. PATIENTS AND METHODS: Thirty patients who underwent cataract surgery were included in the study. A clinical examination (OSDI questionnaire, measurement of tear break-up time (TBUT), corneal staining, Meibomian gland expression test) and a paraclinical evaluation (Meibomian gland loss [MGL] measured using ImageJ on Meibography, conjunctival hyperemia and non-invasive keratograph break-up time [NIK-BUT]) were performed preoperatively and at 1month and 3months after phacoemulsification...
May 16, 2018: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/29770908/level-of-hydrogen-peroxide-affects-expression-and-sub-cellular-localization-of-pax6
#3
Sachin Shukla, Rajnikant Mishra
The Pax6 is a multifunctional pairedbox and homeobox containing transcription factor which is involved in several functions of brain, eyes, and pancreas. It regulates expression of genes involved in cell proliferation, differentiation, inflammation, oxidative stress management, and neuropathy. Dynamic changes in the sub-cellular localization of Pax6 are proposed to regulate its activity, however, the underlying mechanism remains poorly understood. The oxidative stress mediated changes were studied in sub-cellular localization of Pax6 in cultured cells derived from the eye (cornea) and pancreas...
May 16, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29770879/no-difference-in-the-graft-shift-between-a-round-and-a-rounded-rectangular-femoral-tunnel-for-anterior-cruciate-ligament-reconstruction-an-experimental-study
#4
Yasushi Takata, Junsuke Nakase, Takeshi Oshima, Kengo Shimozaki, Kazuki Asai, Hiroyuki Tsuchiya
INTRODUCTION: We developed a novel technique of creating a rounded rectangular femoral bone tunnel for anatomical, single-bundle, autologous hamstring tendon anterior cruciate ligament (ACL) reconstruction. Although this tunnel has many advantages, its non-circular shape has raised concerns regarding excessive graft shift within the bone tunnel. This study aimed to compare the graft shift between round and rounded rectangular tunnels using a graft diameter tester for simulating the femoral bone tunnel...
May 16, 2018: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/29769037/a-method-for-estimating-relative-changes-in-the-synaptic-density-in-drosophila-central-nervous-system
#5
Dipti Rai, Swagata Dey, Krishanu Ray
BACKGROUND: Synapse density is an essential indicator of development and functioning of the central nervous system. It is estimated indirectly through the accumulation of pre and postsynaptic proteins in tissue sections. 3D reconstruction of the electron microscopic images in serial sections is one of the most definitive means of estimating the formation of active synapses in the brain. It is tedious and highly skill-dependent. Confocal imaging of whole mounts or thick sections of the brain provides a natural alternative for rapid gross estimation of the synapse density in large areas...
May 16, 2018: BMC Neuroscience
https://www.readbyqxmd.com/read/29765587/variation-in-the-degree-of-reciprocal-herkogamy-affects-the-degree-of-legitimate-pollination-in-a-distylous-species
#6
Xian-Feng Jiang, Xing-Fu Zhu, Qing-Jun Li
Distyly is a widespread floral polymorphism characterized by the flowers within a population showing reciprocal placement of the anthers and stigma. Darwin hypothesizes that distyly evolves to promote precise pollen transfer between morphs. Primula chungensis exhibits two types of anther heights, and these two types of anthers show pollen of two different size classes. To understand whether the stigma could capture more pollen grains from the anthers of the pollen donor as the separation between the stigma of pollen receiver and the anther of pollen donor decreased, the present research assessed the source of the pollen load in a series of open-pollinated flowers with continuous variation of style lengths...
June 2018: AoB Plants
https://www.readbyqxmd.com/read/29764932/distribution-of-mast-cell-subtypes-in-interstitial-cystitis-implications-for-novel-diagnostic-and-therapeutic-strategies
#7
Shabana T Malik, Brian R Birch, David Voegeli, Mandy Fader, Vipul Foria, Alan J Cooper, Andrew F Walls, Bashir A Lwaleed
AIMS: To identify the presence and geographical distribution of mast cell (MC) subtypes: MCT (tryptase positive-chymase negative) and MCTC (tryptase positive-chymase positive) in bladder tissue. METHODS: Bladder tissue was obtained from patients with painful bladder syndrome/interstitial cystitis (n=14) and normal histology from University Hospital Southampton tissue bank. Sequential tissue slices were immunohistochemically stained for MC subtypes using anti-MC tryptase (for MCT and MCTC ) and anti-MC chymase (for MCTC )...
May 15, 2018: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29761487/the-phytoestrogen-genistein-prevents-trabecular-bone-loss-and-affects-thyroid-follicular-cells-in-a-male-rat-model-of-osteoporosis
#8
Branko Filipović, Branka Šošić-Jurjević, Vladimir Ajdžanović, Jasmina Živanović, Milica Manojlović-Stojanoski, Nataša Nestorović, Nataša Ristić, Svetlana Trifunović, Verica Milošević
As a major phytoestrogen of soy, genistein effectively prevents bone loss in both humans and rat models of osteoporosis. However, although the bone-sparing effects of genistein are achieved directly through estrogen receptors, its mode of action on bone by modulation of other endocrine functions is not entirely clear. Thus, thyroid hormones and calcitonin (CT) have an essential influence on bone metabolism. Besides its action on bones, in this study we examined the effect of genistein on the activity of two different endocrine cell populations, thyroid follicular and C-cells...
May 14, 2018: Journal of Anatomy
https://www.readbyqxmd.com/read/29753580/acceleration-of-diabetic-wound-healing-with-adipose-derived-stem-cells-endothelial-differentiated-stem-cells-and-topical-conditioned-medium-therapy-in-a-swine-model
#9
Robin F Irons, Kevin W Cahill, Deviney A Rattigan, Joseph H Marcotte, Marc W Fromer, Shaohua Chang, Ping Zhang, Eric M Behling, Kathryn C Behling, Francis J Caputo
OBJECTIVE: The purpose of our study was to investigate the effect of adipose-derived stem cells (ASCs), endothelial-differentiated ASCs (EC/ASCs), and various conditioned media (CM) on wound healing in a diabetic swine model. We hypothesized that ASC-based therapies would accelerate wound healing. METHODS: Diabetes was induced in four Yorkshire swine through intravenous injection of streptozotocin. ASCs were harvested from flank fat and cultured in either M199 or EGM-2 medium...
May 9, 2018: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29752492/novel-antidepressant-effects-of-paeonol-alleviate-neuronal-injury-with-concomitant-alterations-in-bdnf-rac1-and-rhoa-levels-in-chronic-unpredictable-mild-stress-rats
#10
Xiu-Ling Zhu, Jing-Jing Chen, Fei Han, Chuan Pan, Ting-Ting Zhuang, Ya-Fei Cai, Ya-Ping Lu
RATIONALE: Increasing evidence has suggested that major depressive disorder (MDD) is highly associated with brain-derived neurotrophic factor (BDNF) levels, dendrites atrophy, and loss of dendritic spines, especially in emotion-associated brain regions including the hippocampus. Paeonol is a kind of polyphenols natural product with a variety of therapeutic effects. Recent studies have reported its antidepressant effects. However, it is unclear what signaling pathways contribute to improve MDD...
May 12, 2018: Psychopharmacology
https://www.readbyqxmd.com/read/29750093/laser-phototherapy-induces-angiogenesis-in-the-periodontal-tissue-after-delayed-tooth-replantation-in-rats
#11
Felipe-de Souza Matos, Fernanda-de Jesus Godolphim, Ricardo-Luiz-Cavalcanti Albuquerque-Júnior, Luiz-Renato Paranhos, Sigmar-de Mello Rode, Cláudio-Antonio-Talge Carvalho, Maria-Amália-Gonzaga Ribeiro
Background: Laser phototherapy (LPT) has been suggested as a new therapeutic tool to improve the repair of replanted teeth. However, its effects and mechanism of action are not yet completely understood. Objectives: This study evaluated histologically the effect of laser phototherapy (LPT) λ808 and λ660 nm on angiogenesis in the periodontal tissue of replanted teeth in rats. Material and Methods: Twenty maxillary right incisors were extracted from twenty Wistar rats and randomly assigned to four groups (n = 5): PN - teeth were stored in paper napkin for 45 min; WM - teeth were immersed in 20 ml of UHT whole cow milk for 45 min; PNL and WML - teeth received the same treatment described for PN and WM, respectively, plus LPT at λ808 and λ660 nm...
April 2018: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/29744916/human-organotypic-retinal-flat-mount-culture-horfc-as-a-model-for-retinitis-pigmentosa11
#12
Leila Azizzadeh Pormehr, Narsis Daftarian, Shahin Ahmadian, Mozhgan Rezaei Kanavi, Hamid Ahmadieh, Mahshid Shafiezadeh
The splicing factor PRPF31 is the most commonly mutated general splicing factor in the retinitis pigmentosa. We used a rapid, convenient and cost effective transfection method with an efficient PRPF31 knockdown in HORFC in order to study the effect of PRPF31 downregulation on retinal gene expressions in an ex vivo model. Modified calcium phosphate method was used to transfect HORFC by PRPF31 siRNA. Different times and doses of siRNA for transfection were assayed and optimum condition was obtained. PRPF31 mRNA and protein downregulation were assessed by qRTPCR and Western blot...
May 10, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29724801/direct-synthesis-of-radioactive-gold-nanoparticles-using-a-research-nuclear-reactor
#13
Maria Camila Garcia Toro, Joshua P Schlegel, Carlos H C Giraldo
We report the synthesis of radioactive gold nanoparticles in a single step process with appropriate activity and size to be used in cancer treatment and diagnosis. Methods: A 2 mM solution of gold chloride (HAuCl4), 1 mM of polyvinylpyrrolidone (PVP, M.W: 360,000), and 60 mM of 2-propanol was prepared in deionized water. Seven different samples of the solution were irradiated in a total neutron flux of 7.45 x 1012 n/cm2 s in a research reactor for 0.5, 1, 3, 5, 10, 30, and 60 minutes. The resulting nanoparticles were characterized for morphology and chemical composition using a Transmission Electron Microscopy (TEM) and ImageJ...
May 3, 2018: Journal of Nuclear Medicine Technology
https://www.readbyqxmd.com/read/29710557/quantification-of-the-traffic-generated-particulate-matter-capture-by-plant-species-in-a-living-wall-and-evaluation-of-the-important-leaf-characteristics
#14
Udeshika Weerakkody, John W Dover, Paul Mitchell, Kevin Reiling
Traffic-generated particulate matter (PM) is a significant fraction of urban PM pollution and little is known about the use of living walls as a short-term strategy to reduce this pollution. The present study evaluated the potential of twenty living wall plants to reduce traffic-based PM using a living wall system located along a busy road in Stoke-on-Trent, UK. An Environmental Scanning Electron Microscope (ESEM) and ImageJ software were employed to quantify PM accumulation on leaves (PM1 , PM2.5 and PM10 ) and their elemental composition was determined using Energy Dispersive X-ray (EDX)...
April 20, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29700782/fabrication-and-testing-of-electrospun-polyurethane-blended-with-chitosan-nanoparticles-for-vascular-graft-applications
#15
Ranjeeta Subramaniam, Mohan Prasath Mani, Saravana Kumar Jaganathan
In this study, a small vascular graft based on polyurethane (PU) blended with chitosan (Ch) nanoparticles was fabricated using electrospinning technique. Initially, the chitosan nanoparticles were synthesized using ionic gelation method. UV-Vis spectrophotometer confirmed the presence of synthesized Ch nanoparticles by exhibiting absorption peak at 288 nm and the Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the existence of the chitosan. Further, the synthesized Ch nanoparticles showed size diameter in the range of 134 ± 58 nm as measured using ImageJ...
April 26, 2018: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/29700758/assessing-sarcopenia-with-vastus-lateralis-muscle-ultrasound-an-operative-protocol
#16
Andrea Ticinesi, Marco V Narici, Fulvio Lauretani, Antonio Nouvenne, Elena Colizzi, Marco Mantovani, Andrea Corsonello, Francesco Landi, Tiziana Meschi, Marcello Maggio
BACKGROUND: Muscle ultrasound (MUS) has so far not been implemented for sarcopenia assessment in clinical geriatric practice due to allegedly low reproducibility of results in the absence of standardization of procedures. However, rigorous and standardized application of this technique yields highly reproducible results. Its application, especially if integrated with clinical evaluation and comprehensive geriatric assessment, proofs very useful for rapidly obtaining information on muscle mass and architecture...
April 26, 2018: Aging Clinical and Experimental Research
https://www.readbyqxmd.com/read/29695321/increased-number-of-tissue-factor-protein-expressing-thrombocytes-in-canine-idiopathic-immune-mediated-hemolytic-anemia
#17
Imke Hennink, Martin W van Leeuwen, Louis C Penning, Christine J Piek
Dogs suffering from canine idiopathic immune mediated hemolytic anemia (cIIMHA) are at great risk of dying particularly in the first two weeks after the diagnosis is made. This high mortality risk may be associated with the development of thromboembolism (TE) and/or disseminated intravascular coagulation (DIC) resulting in organ failure. The exact mechanism of the development of TE and/or DIC in cIIMHA is still undetermined. Therefore, this study investigates the presence of tissue factor (TF) in thrombocytes of dogs suffering from cIIMHA, using OptiPrep™ for the isolation of blood cells and immunocytochemistry (ICC) to visualize TF on thrombocytes...
February 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29685773/dynamic-tracking-of-purkinje-images-for-the-comparison-of-pseudophakodonesis-between-in-the-bag-and-secondary-fixated-intraocular-lenses
#18
Dhivya Ashok Kumar, Amar Agarwal, Nivean Madhivanan, Soundari Sivagnanam, Radika Chandrasekar, Srinivasan Venugopal
PURPOSE: To compare the pseudophakodonesis of in-the-bag intraocular lens (IOL) and a secondary fixated IOL by tracking the Purkinje image IV. SETTING: Dr. Agarwal's Eye Hospital and Eye Research Centre, Chennai, India. DESIGN: Prospective case series. METHODS: The IOL movement was recorded via slitlamp videography (DC3), streamed in video editor (Pinnacle), and evaluated by ImageJ analysis. The positional difference of Purkinje IV in relation to the stationary image Purkinje I at 3 random timeframes was measured...
April 20, 2018: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29676511/role-of-the-high-mobility-group-box-1-signalling-axes-via-the-receptor-for-advanced-glycation-end-products-and-toll-like-receptor-4-in-the-immunopathology-of-oral-lichen-planus-a-potential-drug-target
#19
Abdelhakim Salem, Rabeia Almahmoudi, Mari Vehviläinen, Tuula Salo
High mobility group box 1 (HMGB1) is an extremely conserved DNA-binding protein that stabilizes nucleosomes and facilitates gene transcription in mammalian cells. When released extracellularly, HMGB1 becomes an alarmin that can mediate systemic diseases. High mobility group box 1 signals via two main receptors: receptor for advanced glycation end-products (RAGE) and toll-like receptor-4 (TLR4). We hypothesized that HMGB1 expression is increased in patients with oral lichen planus (OLP) relative to healthy controls...
April 20, 2018: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/29667294/image-quality-and-absorbed-dose-comparison-of-single-and-dual-source-cone-beam-computed-tomography
#20
Hideharu Miura, Shuichi Ozawa, Toshiya Okazue, Atsushi Kawakubo, Kiyoshi Yamada, Yasushi Nagata
PURPOSE: Dual-source cone-beam computed tomography (DCBCT) is currently available in the Vero4DRT image-guided radiotherapy system. We evaluated the image quality and absorbed dose for DCBCT and compared the values with those for single-source CBCT (SCBCT). METHODS: Image uniformity, Hounsfield unit (HU) linearity, image contrast, and spatial resolution were evaluated using a Catphan phantom. The rotation angle for acquiring SCBCT and DCBCT images is 215° and 115°, respectively...
April 17, 2018: Journal of Applied Clinical Medical Physics
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