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Severiano R Silva, Rita Payan-Carreira, Miguel Quaresma, Cristina M Guedes, Ana Sofia Santos
BACKGROUND: In equids, health and welfare depend on body composition. A growing number of equids are now used as leisure and companion animals, and often found overfeed. The need for a close monitoring of body fatness led to the search for tools allowing a rapid and non-invasive estimation of fatness. This study intends to assess real-time ultrasonography (RTU) usefulness in establishing a relationship between ultrasound measures of subcutaneous fat-plus-skin thickness (SF-Skin) and body condition score (BCS) in horses and donkeys...
October 20, 2016: Acta Veterinaria Scandinavica
Gerardo Mendoza-Azpur, Miguel Lau, Erick Valdivia, Jorge Rojas, Henry Muñoz, Myron Nevins
In oral rehabilitation, crown-to-root ratio is accepted as an indicator of bone loss, and this concept was transferred to implants. Recent studies have indicated that there is no significant difference between short and standard implants. The aim of this study was to compare marginal bone-level alteration through radiographic evaluation and clinical parameters between short and standard implants supporting single crowns. This prospective clinical trial study included 82 systemically healthy, nonsmoking subjects...
November 2016: International Journal of Periodontics & Restorative Dentistry
Stephania Caroline Rodolfo Silva, Aion Mangino Messias, Filipe de Oliveira Abi-Rached, Raphael Freitas de Souza, José Maurício Dos Santos Nunes Reis
This study evaluated the accuracy of gypsum casts after different impression techniques and double pouring. Ten patients were selected and for each one it was obtained 5 partial putty/wash impressions with vinyl polysiloxane (VPS) material from teeth #13 to #16 with partial metal stock trays. The following techniques were performed: (1) one-step; two-step relief with: (2) PVC film; (3) slow-speed tungsten carbide bur and scalpel blade, (4) small movements of the tray and (5) without relief-negative control...
2016: PloS One
John F Antiabong, Mafora G Ngoepe, Adakole S Abechi
AIM: The interpretation of conventional polymerase chain reaction (PCR) assay results is often limited to either positive or negative (non-detectable). The more robust quantitative PCR (qPCR) method is mostly reserved for quantitation studies and not a readily accessible technology in laboratories across developing nations. The aim of this study was to evaluate a semi-quantitative method for conventional PCR amplicons using digital image analysis of electrophoretic gel. The potential applications are also discussed...
September 2016: Veterinary World
Veeran Veeravarmal, Ravi David Austin, Nagini Siddavaram, Sambanthan Thiruneelakandan, Mohamed Hanifa Mohamed Nassar
CONTEXT: The epithelium atrophy, as the oral submucous fibrosis (OSMF) progresses, is believed to be an after effect of stromal fibrosis, hyalinization, decrease in vascularity and cellularity and is considered as "ischemic atrophy." Due to hypoxia, caspase-3 get activation and subsequent decrease in viable cell count can occur. AIMS AND OBJECTIVES: To determine caspase-3 expression in various grades of OSMF and oral squamous cell carcinoma (OSCC) to find out whether upregulation of apoptosis is responsible for the epithelial changes in OSMF...
September 2016: Journal of Oral and Maxillofacial Pathology: JOMFP
Ivan R Ilic, Nikola M Stojanović, Pavle J Randjelović, Marina N Mihajlović, Niko S Radulović, Ratko S Ilić
BACKGROUND: Invasive lobular breast cancer (ILC) is the second most frequent form of breast cancer. While cancer cells are regularly investigated, tumor stroma represents a highly unexplored field. AIMS: The aim of this study is to perform a detailed investigation of clinical, immunohistochemical, and morphometric characteristics of desmoplastic (D) and nondesmoplastic (ND) ILC. MATERIALS AND METHODS: This study included twenty cases of ILC that were divided into two groups designated as D and ND groups...
October 2016: Indian Journal of Pathology & Microbiology
Sang-Mok Lee, Hyuk Jin Choi, Heejin Choi, Mee Kum Kim, Won Ryang Wee
: BACKGROUND: Though the development and fitting of scleral contact lenses are expanding steadily, there is no simple method to provide scleral metrics for scleral contact lens fitting yet. The aim of this study was to establish formulae for estimation of the axial radius of curvature (ARC) of the anterior sclera using ocular biometric parameters that can be easily obtained with conventional devices. METHODS: A semi-automated stitching method and a computational analysis tool for calculating ARC were developed by using the ImageJ and MATLAB software...
October 7, 2016: BMC Ophthalmology
Jason T Hamamoto, Timothy Leroux, Jorge Chahla, Sanjeev Bhatia, John D Higgins, Anthony A Romeo, Adam B Yanke, Nikhil N Verma
The preoperative assessment of anterior glenoid bone loss is a critical step in surgical planning for patients with recurrent anterior glenohumeral instability. The structural integrity of the glenoid has been identified as one of the most important factors influencing the success of operative repair. The currently accepted gold standard for glenoid structural assessment among most orthopaedic surgeons is the use of 3-dimensional reconstructed computed tomography images with the humeral head digitally subtracted, yielding an en face sagittal oblique view of the glenoid...
August 2016: Arthroscopy Techniques
Gabriela Tavares, Manuella Martins, Joana Sofia Correia, Vanessa Morais Sardinha, Sónia Guerra-Gomes, Sofia Pereira das Neves, Fernanda Marques, Nuno Sousa, João Filipe Oliveira
Astrocytes display important features that allow them to maintain a close dialog with neurons, ultimately impacting brain function. The complex morphological structure of astrocytes is crucial to the role of astrocytes in brain networks. Therefore, assessing morphologic features of astrocytes will help provide insights into their physiological relevance in healthy and pathological conditions. Currently available tools that allow the tridimensional reconstruction of astrocytes present a number of disadvantages, including the need for advanced computational skills and powerful hardware, and are either time-consuming or costly...
September 30, 2016: Brain Structure & Function
Linnea K Honeker, Robert A Root, Jon Chorover, Raina M Maier
Metal(loid)-contamination of the environment due to anthropogenic activities is a global problem. Understanding the fate of contaminants requires elucidation of biotic and abiotic factors that influence metal(loid) speciation from molecular to field scales. Improved methods are needed to assess micro-scale processes, such as those occurring at biogeochemical interfaces between plant tissues, microbial cells, and metal(loid)s. Here we present an advanced method that combines fluorescence in situ hybridization (FISH) with synchrotron-based multiple-energy micro-focused X-ray fluorescence microprobe imaging (ME μXRF) to examine colocalization of bacteria and metal(loid)s on root surfaces of plants used to phytostabilize metalliferous mine tailings...
September 29, 2016: Journal of Microbiological Methods
Kyle B Serkies, Reena Garcha, Laura E Tam, Grace M De Souza, Yoav Finer
OBJECTIVE: Assess the modulating effect of matrix metalloproteinase (MMP) inhibition on simulated human salivary enzyme (SHSE)-catalyzed degradation of interfacial fracture-toughness (FT) of self-etched and total-etched resin-dentin interfaces. METHODS: Miniature short-rod FT specimens (N=10/group) containing a resin composite bonded to human dentin, using a self-etch (Easy Bond, EB) or a total-etch (Scotchbond, SB) adhesives, were prepared with and without application of an MMP inhibitor (galardin)...
September 28, 2016: Dental Materials: Official Publication of the Academy of Dental Materials
Ali Ansari, Reema Patel, Kinsey Schultheis, Vesna Naumovski, P I Imoukhuede
One of the limiting factors to the adoption and advancement of personalized medicine is the inability to develop diagnostic tools to probe individual nuances in expression from patient to patient. Current methodologies that try to separate cells to fill this niche result in disruption of physiological expression, making the separation technique useless as a diagnostic tool. In this protocol, we describe the functionalization and optimization of a surface for the cellular capture and release. This functionalized surface integrates biotinylated antibodies with a glass surface functionalized with an aminosilane (APTES), desthiobiotin and streptavidin...
2016: Journal of Visualized Experiments: JoVE
Philipp Genseke, Julian M M Rogasch, Ingo G Steffen, Grit Neumann, Ivayla Apostolova, Juri Ruf, Anke Rißmann, Dagobert Wiemann, Uwe-Bernd Liehr, Martin Schostak, Holger Amthauer, Christian Furth
AIM: After detection of obstructive uropathy (OU), the indication for or against surgery is primarily based on the differential renal function (DRF). This is to compare functional magnetic resonance urography (fMRU) with dynamic renal scintigraphy (DRS) to assess OU and DRF in infants and children. PATIENTS, METHODS: Retrospective analysis in 30 patients (female: 16; male: 14; median age: 5.5 years [0.2-16.5]), divided into subgroup A (age: 0-2 years; n = 16) and B (> 2-17 years; n = 14)...
August 29, 2016: Nuklearmedizin. Nuclear Medicine
Fabio Scarinci, Amr Shaarawy, Ramsudha Narala, Lee M Jampol, Amani A Fawzi
PURPOSE: To quantify external limiting membrane (ELM) disruption and photoreceptor volume over time, using spectral domain optical coherence tomography in eyes with hydroxychloroquine (HCQ) toxic effects after discontinuation. METHODS: We performed a retrospective chart review of patients who were screened for toxic effects of HCQ between January 1, 2009 and August 31, 2014, and identified 10 patients diagnosed as having HCQ retinal toxic effects. Intact ELM and the Bruch membrane were manually traced using ImageJ software and their lengths from each scan of the spectral domain optical coherence tomography macular volume were summed...
October 2016: Retina
Stefan Schob, Peter Voigt, Lionel Bure, Hans-Jonas Meyer, Claudia Wickenhauser, Curd Behrmann, Annekathrin Höhn, Paul Kachel, Henning Dralle, Karl-Titus Hoffmann, Alexey Surov
BACKGROUND: Thyroid carcinomas represent the most frequent endocrine malignancies. Recent studies were able to distinguish malignant from benign nodules of the thyroid gland with diffusion-weighted imaging (DWI). Although this differentiation is undoubtedly helpful, presurgical discrimination between well-differentiated and undifferentiated carcinomas would be crucial to define the optimal treatment algorithm. Therefore, the aim of this study was to investigate if readout-segmented multishot echo planar DWI is able to differentiate between differentiated and undifferentiated subtypes of thyroid carcinomas...
October 2016: Translational Oncology
Jillian M Doyle, Neil Merovitch, Russell C Wyeth, Matthew R Stoyek, Michael Schmidt, Florentin Wilfart, Alan Fine, Roger P Croll
We describe here an automated apparatus that permits rapid conditioning paradigms for zebrafish. Arduino microprocessors were used to control the delivery of auditory or visual stimuli to groups of adult or juvenile zebrafish in their home tanks in a conventional zebrafish facility. An automatic feeder dispensed precise amounts of food immediately after the conditioned stimuli, or at variable delays for controls. Responses were recorded using inexpensive cameras, with the video sequences analysed with ImageJ or Matlab...
September 19, 2016: Behavioural Brain Research
J Nekolová, H Langrová, P Rozsíval, N Jirásková
PURPOSE: To prospectively compare postoperative results of two premium intraocular lenses EnVISTA (Bausch and Lomb) and AcrySof IQ (Alcon), focussing on glistenings and posterior capsule opacification. The evaluation of glistenings was done using Image J software and posterior capsule opacifications were quantified with OSCA system. METHODS: Twenty patients (7 men and 13 women) with bilateral cataract were included. EnVista intraocular lens (IOL) was implanted in one eye and AcrySof IQ IOL in the second eye of each patient...
2016: Ceská a Slovenská Oftalmologie
Ananta Poudel, Jonas L Fowler, Mark C Zielinski, German Kilimnik, Manami Hara
The large size of human tissues requires a practical stereological approach to perform a comprehensive analysis of the whole organ. We have developed a method to quantitatively analyze the whole human pancreas, as one of the challenging organs to study, in which endocrine cells form various sizes of islets that are scattered unevenly throughout the exocrine pancreas. Furthermore, the human pancreas possesses intrinsic characteristics of intra-individual variability, i.e. regional differences in endocrine cell/islet distribution, and marked inter-individual heterogeneity regardless of age, sex and disease conditions including obesity and diabetes...
2016: Scientific Reports
You Na Kim, Jin Hyoung Park, Hungwon Tchah
Purpose. To quantify whole lens and nuclear lens densities using anterior-segment optical coherence tomography (OCT) with a liquid optics interface and evaluate their correlation with Lens Opacities Classification System III (LOCS III) lens grading and corrected distance visual acuity (BCVA). Methods. OCT images of the whole lens and lens nucleus of eyes with age-related nuclear cataract were analyzed using ImageJ software. The lens grade and nuclear density were represented in pixel intensity units (PIU) and correlations between PIU, BCVA, and LOCS III were assessed...
2016: Journal of Ophthalmology
O Wirjadi, Y-J Kim, F Stech, L Bonfert, M Wagner
Image segmentation aims to determine structures of interest inside a digital picture in biomedical sciences. State-of-the art automatic methods however still fail to provide the segmentation quality achievable by humans who employ expert knowledge and use software to mark target structures on an image. Manual segmentation is time-consuming, tedious and suffers from interoperator variability, thus not serving the requirements of daily use well. Therefore, the approach presented here abandons the goal of full-fledged segmentation and settles for the localization of circular objects in photographs (10 training images and 20 testing images with several hundreds of nuclei each)...
September 20, 2016: Journal of Microscopy
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