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https://www.readbyqxmd.com/read/28548095/metabolic-engineering-of-saccharomyces-cerevisiae-for-production-of-very-long-chain-fatty-acid-derived-chemicals
#1
Tao Yu, Yongjin J Zhou, Leonie Wenning, Quanli Liu, Anastasia Krivoruchko, Verena Siewers, Jens Nielsen, Florian David
Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase...
May 26, 2017: Nature Communications
https://www.readbyqxmd.com/read/28545405/genetic-complexity-of-miscanthus-cell-wall-composition-and-biomass-quality-for-biofuels
#2
Tim van der Weijde, Claire L Alvim Kamei, Edouard I Severing, Andres F Torres, Leonardo D Gomez, Oene Dolstra, Chris A Maliepaard, Simon J McQueen-Mason, Richard G F Visser, Luisa M Trindade
BACKGROUND: Miscanthus sinensis is a high yielding perennial grass species with great potential as a bioenergy feedstock. One of the challenges that currently impedes commercial cellulosic biofuel production is the technical difficulty to efficiently convert lignocellulosic biomass into biofuel. The development of feedstocks with better biomass quality will improve conversion efficiency and the sustainability of the value-chain. Progress in the genetic improvement of biomass quality may be substantially expedited by the development of genetic markers associated to quality traits, which can be used in a marker-assisted selection program...
May 25, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28541580/genes-and-gene-clusters-related-to-genotype-and-drought-induced-variation-in-saccharification-potential-lignin-content-and-wood-anatomical-traits-in-populus-nigra%C3%A2
#3
Henning Wildhagen, Shanty Paul, Mike Allwright, Hazel K Smith, Marta Malinowska, Sabine K Schnabel, M João Paulo, Federica Cattonaro, Vera Vendramin, Simone Scalabrin, Dennis Janz, Cyril Douthe, Oliver Brendel, Cyril Buré, David Cohen, Irène Hummel, Didier Le Thiec, Fred van Eeuwijk, Joost J B Keurentjes, Jaume Flexas, Michele Morgante, Paul Robson, Marie-Béatrice Bogeat-Triboulot, Gail Taylor, Andrea Polle
Wood is a renewable resource that can be employed for the production of second generation biofuels by enzymatic saccharification and subsequent fermentation. Knowledge on how the saccharification potential is affected by genotype-related variation of wood traits and drought is scarce. Here, we used three Populus nigra L. genotypes from habitats differing in water availability to (i) investigate the relationships between wood anatomy, lignin content and saccharification and (ii) identify genes and co-expressed gene clusters related to genotype and drought-induced variation in wood traits and saccharification potential...
May 24, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28537253/a-synthetic-biochemistry-platform-for-cell-free-production-of-monoterpenes-from-glucose
#4
Tyler P Korman, Paul H Opgenorth, James U Bowie
Cell-free systems designed to perform complex chemical conversions of biomass to biofuels or commodity chemicals are emerging as promising alternatives to the metabolic engineering of living cells. Here we design a system comprises 27 enzymes for the conversion of glucose into monoterpenes that generates both NAD(P)H and ATP in a modified glucose breakdown module and utilizes both cofactors for building terpenes. Different monoterpenes are produced in our system by changing the terpene synthase enzyme. The system is stable for the production of limonene, pinene and sabinene, and can operate continuously for at least 5 days from a single addition of glucose...
May 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28535471/graphene-oxide-supported-carbon-nanofiber-like-network-derived-from-polyaniline-a-novel-composite-for-enhanced-glucose-oxidase-bioelectrode-performance
#5
Zepeng Kang, Kailong Jiao, Xinping Xu, Ruiyun Peng, Shuqiang Jiao, Zongqian Hu
A three-dimensional architecture of PANI@GO hybrid was synthesized via in-situ polymerization of aniline monomers on the surface of graphene oxide (GO) and carbonized at 1600°C. The SEM images showed that surfaces of planar GO were covered by a compact nanofiber-like polyaniline (PANI) layer which presented an interconnected network. Nanofiber-like PANI on the GO surface was mostly preserved and became the carbon nanofibers (CNFs) after carbonization. The TEM images showed that the carbonized PANI grew seamlessly on the GO surface and served as conductive "network" between interlayers of GO...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28505306/secretion-of-2-3-dihydroxyisovalerate-as-a-limiting-factor-for-isobutanol-production-in-saccharomyces-cerevisiae
#6
Wesley Cardoso Generoso, Martin Brinek, Heiko Dietz, Mislav Oreb, Eckhard Boles
Isobutanol is a superior biofuel compared to ethanol, and it is naturally produced by yeasts. Previously, Saccharomyces cerevisiae has been genetically engineered to improve isobutanol production. We found that yeast cells engineered for a cytosolic isobutanol biosynthesis secrete large amounts of the intermediate 2,3-dihydroxyisovalerate (DIV). This indicates that the enzyme dihydroxyacid dehydratase (Ilv3) is limiting the isobutanol pathway and/or yeast exhibit effective transport systems for the secretion of the intermediate, competing with isobutanol synthesis...
May 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28503197/the-significance-of-proline-and-glutamate-on-butanol-chaotropic-stress-in-bacillus-subtilis-168
#7
Gumpanat Mahipant, Atchara Paemanee, Sittiruk Roytrakul, Junichi Kato, Alisa S Vangnai
BACKGROUND: Butanol is an intensively used industrial solvent and an attractive alternative biofuel, but the bioproduction suffers from its high toxicity. Among the native butanol producers and heterologous butanol-producing hosts, Bacillus subtilis 168 exhibited relatively higher butanol tolerance. Nevertheless, organic solvent tolerance mechanisms in Bacilli and Gram-positive bacteria have relatively less information. Thus, this study aimed to elucidate butanol stress responses that may involve in unique tolerance of B...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28501381/salinity-induced-oxidative-stress-alters-the-physiological-responses-and-improves-the-biofuel-potential-of-green-microalgae-acutodesmus-dimorphus
#8
Kaumeel Chokshi, Imran Pancha, Arup Ghosh, Sandhya Mishra
The main aim of the present study was to analyze salinity stress induced physiological and biochemical changes in a freshwater microalgae Acutodesmus dimorphus. During single-stage cultivation, the accumulations of lipids and carbohydrates increased with an increase in an initial salinity of the culture medium. The carbohydrate and lipid accumulations of 53.30±2.76% and 33.40±2.29%, respectively, were observed in 200mM NaCl added culture. During two-stage cultivation, salinity stress of 200mM was favorable for the growth up to 2days, as suggested by higher biomass, lower levels of oxidative stress biomarkers and no significant changes in the biochemical composition of the cells...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28491136/applications-of-microalgal-biofilms-for-wastewater-treatment-and-bioenergy-production
#9
Ana F Miranda, Narasimhan Ramkumar, Constandino Andriotis, Thorben Höltkemeier, Aneela Yasmin, Simone Rochfort, Donald Wlodkowic, Paul Morrison, Felicity Roddick, German Spangenberg, Banwari Lal, Sanjukta Subudhi, Aidyn Mouradov
BACKGROUND: Microalgae have shown clear advantages for the production of biofuels compared with energy crops. Apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (C, N, and P), heavy metals and micropollutants, and they do not compete with crops for arable lands. However, fundamental barriers to the industrial application of microalgae for biofuel production still include high costs of removing the algae from the water and the water from the algae which can account for up to 30-40% of the total cost of biodiesel production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28486485/hydrogen-peroxide-inhibition-of-bicupin-oxalate-oxidase
#10
John M Goodwin, Hassan Rana, Joan Ndungu, Gaurab Chakrabarti, Ellen W Moomaw
Oxalate oxidase is a manganese containing enzyme that catalyzes the oxidation of oxalate to carbon dioxide in a reaction that is coupled with the reduction of oxygen to hydrogen peroxide. Oxalate oxidase from Ceriporiopsis subvermispora (CsOxOx) is the first fungal and bicupin enzyme identified that catalyzes this reaction. Potential applications of oxalate oxidase for use in pancreatic cancer treatment, to prevent scaling in paper pulping, and in biofuel cells have highlighted the need to understand the extent of the hydrogen peroxide inhibition of the CsOxOx catalyzed oxidation of oxalate...
2017: PloS One
https://www.readbyqxmd.com/read/28484516/on-the-pathways-feeding-the-h2-production-process-in-nutrient-replete-hypoxic-conditions-commentary-on-the-article-low-oxygen-levels-contribute-to-improve-photohydrogen-production-in-mixotrophic-non-stressed-chlamydomonas-cultures-by-jurado-oller-et-al-biotechnology
#11
EDITORIAL
Alberto Scoma, Szilvia Z Tóth
BACKGROUND: Under low O2 concentration (hypoxia) and low light, Chlamydomonas cells can produce H2 gas in nutrient-replete conditions. This process is hindered by the presence of O2, which inactivates the [FeFe]-hydrogenase enzyme responsible for H2 gas production shifting algal cultures back to normal growth. The main pathways accounting for H2 production in hypoxia are not entirely understood, as much as culture conditions setting the optimal redox state in the chloroplast supporting long-lasting H2 production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28484515/cultivation-of-algal-biofilm-using-different-lignocellulosic-materials-as-carriers
#12
Qi Zhang, Cuixia Liu, Yubiao Li, Zhigang Yu, Zhihua Chen, Ting Ye, Xun Wang, Zhiquan Hu, Shiming Liu, Bo Xiao, Shiping Jin
BACKGROUND: Algal biofilm technology is recently supposed to be a promising method to produce algal biomass as the feedstock for the production of biofuels. However, the carrier materials currently used to form algal biofilm are either difficult to be obtained at a low price or undurable. Commercialization of the biofilm technology for algal biomass production extremely requires new and inexpensive materials as biofilm carriers with high biomass production performances. RESULTS: Four types of lignocellulosic materials were investigated to evaluate their performance of acting as carriers for algal cells attachment and the relevant effects on the algal biomass production in this study...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28483385/acclimation-to-nacl-and-light-stress-of-heterotrophic-chlamydomonas-reinhardtii-for-lipid-accumulation
#13
Jianhua Fan, Lvhong Zheng
Salt stress has been proven very effective in enhancing the lipid content among many photoautotrophically grown microalgae species including marine and freshwater algae. Nevertheless, its effect on heterotrophic grown cells and lipid accumulation is scarcely known. This study sought to demonstrate a new train of thought for cost-effective biofuels production by heterotrophic culture of Chlamydomonas reinhardtii coupling with subsequent salt and light stress. NaCl treatments (25-200 mM) gradually suppressed the cell growth...
May 5, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28482543/self-assembly-of-glucose-oxidase-on-reduced-graphene-oxide-magnetic-nanoparticles-nanocomposite-based-direct-electrochemistry-for-reagentless-glucose-biosensor
#14
Saithip Pakapongpan, Rungtiva P Poo-Arporn
A novel approach of the immobilization of a highly selective and stable glucose biosensor based on direct electrochemistry was fabricated by a self-assembly of glucose oxidase (GOD) on reduced graphene oxide (RGO) covalently conjugated to magnetic nanoparticles (Fe3O4 NPs) modified on a magnetic screen-printed electrode (MSPE). The RGO-Fe3O4 nanocomposite has remarkable enhancement in large surface areas, is favorable environment for enzyme immobilization, facilitates electron transfer between enzymes and electrode surfaces and possesses superparamagnetism property...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28469705/suppression-of-cinnamoyl-coa-reductase-increases-the-level-of-monolignol-ferulates-incorporated-into-maize-lignins
#15
Rebecca A Smith, Cynthia L Cass, Mona Mazaheri, Rajandeep S Sekhon, Marlies Heckwolf, Heidi Kaeppler, Natalia de Leon, Shawn D Mansfield, Shawn M Kaeppler, John C Sedbrook, Steven D Karlen, John Ralph
BACKGROUND: The cell wall polymer lignin provides structural support and rigidity to plant cell walls, and therefore to the plant body. However, the recalcitrance associated with lignin impedes the extraction of polysaccharides from the cell wall to make plant-based biofuels and biomaterials. The cell wall digestibility can be improved by introducing labile ester bonds into the lignin backbone that can be easily broken under mild base treatment at room temperature. The FERULOYL-CoA MONOLIGNOL TRANSFERASE (FMT) enzyme, which may be naturally found in many plants, uses feruloyl-CoA and monolignols to synthesize the ester-linked monolignol ferulate conjugates...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28469179/highly-selective-and-sensitive-self-powered-glucose-sensor-based-on-capacitor-circuit
#16
Gymama Slaughter, Tanmay Kulkarni
Enzymatic glucose biosensors are being developed to incorporate nanoscale materials with the biological recognition elements to assist in the rapid and sensitive detection of glucose. Here we present a highly sensitive and selective glucose sensor based on capacitor circuit that is capable of selectively sensing glucose while simultaneously powering a small microelectronic device. Multi-walled carbon nanotubes (MWCNTs) is chemically modified with pyrroloquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BOD) at anode and cathode, respectively, in the biofuel cell arrangement...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28465173/glucose-6-phosphate-dehydrogenase-as-a-target-for-highly-efficient-fatty-acid-biosynthesis-in-microalgae-by-enhancing-nadph-supply
#17
Jiao Xue, Srinivasan Balamurugan, Da-Wei Li, Yu-Hong Liu, Hao Zeng, Lan Wang, Wei-Dong Yang, Jie-Sheng Liu, Hong-Ye Li
Oleaginous microalgae have great prospects in the fields of feed, nutrition, biofuel, etc. However, biomass and lipid productivity in microalgae remain a major economic and technological bottleneck. Here we present a novel regulatory target, glucose-6-phosphate dehydrogenase (G6PD) from the pentose phosphate pathway (PPP), in boosting microalgal lipid accumulation. G6PD, involved in the formation of NADPH demanded in fatty acid biosynthesis as reducing power, was characterized in oleaginous microalga Phaeodactylum tricornutum...
April 30, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28465060/biosynthesis-of-the-fatty-acid-isopropyl-esters-by-engineered-escherichia-coli
#18
Hong Pan, Lihua Zhang, Xun Li, Daoyi Guo
The fatty acid methyl esters and fatty acid ethyl esters are known as biodiesels which are considered to be renewable, nontoxic and biodegradable biofuels. However, the conventional biodiesels show a high crystallization temperature which is one of the most critical obstacles against the widespread biodiesel usage. The high crystallization temperature of biodiesel can be reduced by replacing the methyl or ethyl ester with an isopropyl moiety. Here we report on a strategy to establish biosynthesis of the fatty acid isopropyl esters(FAIPEs) from the simple substrate glucose in Escherichia coli with heterologous coexpression of atoB encoded acetyl-CoA acetyltransferase and atoAD encode acetoacetyl-CoA transferase from E...
July 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28464851/pollen-mediated-gene-flow-from-transgenic-to-non-transgenic-switchgrass-panicum-virgatum-l-in-the-field
#19
Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R Johnson, Micaha Hanson, Jason N Burris, Charles Kwit, C Neal Stewart
BACKGROUND: Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter cell walls for more facile bioprocessing and biofuel yield. Gene flow from transgenic cultivars would likely be of regulatory concern. In this study we investigated pollen-mediated gene flow from transgenic to nontransgenic switchgrass in a 3-year field experiment performed in Oliver Springs, Tennessee, U...
May 2, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28464284/engineering-brevibacterium-flavum-for-the-production-of-renewable-bioenergy-c4-c5-advanced-alcohols
#20
HaiFeng Su, JiaFu Lin, YuanHong Wang, Qiao Chen, GuangWei Wang, FuRong Tan
Biosynthesis of advanced biofuels by engineered non-natural microorganisms has been proposed to be the most promising approach for the replacement of dwindling fossil fuel resources. Brevibacterium flavum (Bf) is a model brevibacterium aerobe which lacks basic and applied research that could enable this species to produce biofuels. There are no reports regarding engineering this microorganism to produce advanced alcohols before. Here, for the first time, we developed the bacterium as a novel biosynthetic platform for advanced alcohols production via the mutagenesis and engineering to produce 2-ketoacids derived alcohols...
May 2, 2017: Biotechnology and Bioengineering
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