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https://www.readbyqxmd.com/read/28103008/quantitative-tracking-of-combinatorially-engineered-populations-with-multiplexed-binary-assemblies
#1
Ramsey Zeitoun, Gur Pines, William C Grau, Ryan T Gill
Advances in synthetic biology and genomics have enabled full-scale genome engineering efforts on laboratory timescales. However, the absence of sufficient approaches for mapping engineered genomes at system-wide scales onto performance has limited the adoption of more sophisticated algorithms for engineering complex biological systems. Here we report on the development and application of a robust approach to quantitatively map combinatorially engineered populations at scales up to several dozen target sites...
January 19, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28102313/cultivation-and-energy-efficient-harvesting-of-microalgae-using-thermoreversible-sol-gel-transition
#2
Bendy Estime, Dacheng Ren, Radhakrishna Sureshkumar
Microalgae represent a promising source of renewable biomass for the production of biofuels and valuable chemicals. However, energy efficient cultivation and harvesting technologies are necessary to improve economic viability. A Tris-Acetate-Phosphate-Pluronic (TAPP) medium that undergoes a thermoreversible sol-gel transition is developed to efficiently culture and harvest microalgae without affecting the productivity as compared to that in traditional culture in a well-mixed suspension. After seeding microalgae in the TAPP medium in a solution phase at 15 °C, the temperature is increased by 7 °C to induce gelation...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28097785/down-regulation-of-bnda1-whose-gene-locus-is-associated-with-the-seeds-weight-improves-the-seeds-weight-and-organ-size-in-brassica-napus
#3
Jie-Li Wang, Min-Qiang Tang, Sheng Chen, Xiang-Feng Zheng, Hui-Xian Mo, Sheng-Jun Li, Zheng Wang, Ke-Ming Zhu, Li-Na Ding, Sheng-Yi Liu, Yun-Hai Li, Xiao-Li Tan
Brassica napus L. is an important oil crop worldwide, and is the main raw material for biofuel. Seed weight and seed size are the main contributors to seed yield. DA1 (DA means big in Chinese) is an ubiquitin receptor and negatively regulates seed size. Down-regulation of AtDA1 in Arabidopsis leads to larger seeds and organs by increasing cell proliferation in integuments. In this study, BnDA1was down-regulated in B. napus by over expressed of AtDA1(R358K) , which is a functional deficiency of DA1 with an arginine-to-lysine mutation at the 358(th) amino acid...
January 17, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28096492/identification-of-glutaminyl-cyclase-genes-involved-in-pyroglutamate-modification-of-fungal-lignocellulolytic-enzymes
#4
Vincent W Wu, Craig M Dana, Anthony T Iavarone, Douglas S Clark, N Louise Glass
: The breakdown of plant biomass to simple sugars is essential for the production of second-generation biofuels and high-value bioproducts. Currently, enzymes produced from filamentous fungi are used for deconstructing plant cell wall polysaccharides into fermentable sugars for biorefinery applications. A post-translational N-terminal pyroglutamate modification observed in some of these enzymes occurs when N-terminal glutamine or glutamate is cyclized to form a five-membered ring. This modification has been shown to confer resistance to thermal denaturation for CBH-1 and EG-1 cellulases...
January 17, 2017: MBio
https://www.readbyqxmd.com/read/28080037/global-metabolic-engineering-of-glycolytic-pathway-via-multi-copy-integration-in-saccharomyces-cerevisiae
#5
Ryosuke Yamada, Kazuki Wakita, Hiroyasu Ogino
The use of renewable feedstocks for producing biofuels and bio-based chemicals by engineering metabolic pathways of yeast Saccharomyces cerevisiae has recently become an attractive option. Many researchers attempted to increase glucose consumption rate by overexpressing some glycolytic enzymes because most target bio-based chemicals are derived through glycolysis. However these attempts have met with little success. In this study, to create a S. cerevisiae strain with high glucose consumption rate, we used multi-copy integration to develop a global metabolic engineering strategy...
January 12, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28078719/carbon-nanotube-supported-bio-inspired-nickel-catalyst-and-its-integration-in-hybrid-hydrogen-air-fuel-cells
#6
Solène Gentil, Noémie Lalaoui, Arnab Dutta, Yannig Nedellec, Serge Cosnier, Wendy J Shaw, Vincent Artero, Alan Le Goff
A biomimetic nickel bis-diphosphine complex incorporating the amino acid arginine in the outer coordination sphere was immobilized on modified carbon nanotubes (CNTs) through electrostatic interactions. The functionalized redox nanomaterial exhibits reversible electrocatalytic activity for the H2 /2 H(+) interconversion from pH 0 to 9, with catalytic preference for H2 oxidation at all pH values. The high activity of the complex over a wide pH range allows us to integrate this bio-inspired nanomaterial either in an enzymatic fuel cell together with a multicopper oxidase at the cathode, or in a proton exchange membrane fuel cell (PEMFC) using Pt/C at the cathode...
January 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28074108/cellulolytic-robolector-towards-an-automated-high-throughput-screening-platform-for-recombinant-cellulase-expression
#7
Martina Mühlmann, Martin Kunze, Joaquim Ribeiro, Bertram Geinitz, Christian Lehmann, Ulrich Schwaneberg, Ulrich Commandeur, Jochen Büchs
BACKGROUND: Cellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the enzymes. To overcome this draw back, a lot of effort is put into the area of protein engineering, to modify these enzymes by directed evolution or rational design. Huge clone libraries are constructed and have to be screened for improved variants. High-throughput screening is the method of choice to tackle this experimental effort, but up to now only a few process steps are adapted to automated platforms and little attention has been turned to the reproducibility of clone rankings...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28070721/enhanced-xylose-fermentation-by-engineered-yeast-expressing-nadh-oxidase-through-high-cell-density-inoculums
#8
Guo-Chang Zhang, Timothy L Turner, Yong-Su Jin
Accumulation of reduced byproducts such as glycerol and xylitol during xylose fermentation by engineered Saccharomyces cerevisiae hampers the economic production of biofuels and chemicals from cellulosic hydrolysates. In particular, engineered S. cerevisiae expressing NADPH-linked xylose reductase (XR) and NAD(+)-linked xylitol dehydrogenase (XDH) produces substantial amounts of the reduced byproducts under anaerobic conditions due to the cofactor difference of XR and XDH. While the additional expression of a water-forming NADH oxidase (NoxE) from Lactococcus lactis in engineered S...
January 9, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28066510/rapid-in-vivo-lipid-carbohydrate-quantification-of-single-microalgal-cell-by-raman-spectral-imaging-to-reveal-salinity-induced-starch-to-lipid-shift
#9
Liang-da Chiu, Shih-Hsin Ho, Rintaro Shimada, Nan-Qi Ren, Takeaki Ozawa
BACKGROUND: Lipid/carbohydrate content and ratio are extremely important when engineering algal cells for liquid biofuel production. However, conventional methods for such determination and quantification are not only destructive and tedious, but also energy consuming and environment unfriendly. In this study, we first demonstrate that Raman spectroscopy is a clean, fast, and accurate method to simultaneously quantify the lipid/carbohydrate content and ratio in living microalgal cells...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28066508/a-molecular-genetic-toolbox-for-yarrowia-lipolytica
#10
Erin L Bredeweg, Kyle R Pomraning, Ziyu Dai, Jens Nielsen, Eduard J Kerkhoven, Scott E Baker
BACKGROUND: Yarrowia lipolytica is an ascomycete yeast used in biotechnological research for its abilities to secrete high concentrations of proteins and accumulate lipids. Genetic tools have been made in a variety of backgrounds with varying similarity to a comprehensively sequenced strain. RESULTS: We have developed a set of genetic and molecular tools in order to expand capabilities of Y. lipolytica for both biological research and industrial bioengineering applications...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28063146/pretreatment-optimization-of-the-biomass-of-microcystis-aeruginosa-for-efficient-bioethanol-production
#11
Muhammad Imran Khan, Moon Geon Lee, Jin Hyuk Shin, Jong Deog Kim
Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28058634/alkane-biosynthesis-by-aspergillus-carbonarius-item-5010-through-heterologous-expression-of-synechococcus-elongatus-acyl-acp-coa-reductase-and-aldehyde-deformylating-oxygenase-genes
#12
Malavika Sinha, István Weyda, Annette Sørensen, Kenneth S Bruno, Birgitte K Ahring
In this study we describe the heterologous expression of the recently identified cyanobacterial pathway for long chain alkane biosynthesis, involving the reduction of fatty acyl-ACP to fatty aldehyde and the subsequent conversion of this into alkanes, in the filamentous fungus Aspergillus carbonarius ITEM 5010. Genes originating from Synechococcus elongatus strain PCC7942, encoding acyl-ACP/CoA reductase and aldehyde deformylating oxygenase enzymes, were successfully expressed in A. carbonarius, which lead to the production of pentadecane and heptadecane, alkanes that have not been previously produced by this fungus...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28054656/fabrication-of-enzyme-based-coatings-on-intact-multi-walled-carbon-nanotubes-as-highly-effective-electrodes-in-biofuel-cells
#13
Byoung Chan Kim, Inseon Lee, Seok-Joon Kwon, Youngho Wee, Ki Young Kwon, Chulmin Jeon, Hyo Jin An, Hee-Tae Jung, Su Ha, Jonathan S Dordick, Jungbae Kim
CNTs need to be dispersed in aqueous solution for their successful use, and most methods to disperse CNTs rely on tedious and time-consuming acid-based oxidation. Here, we report the simple dispersion of intact multi-walled carbon nanotubes (CNTs) by adding them directly into an aqueous solution of glucose oxidase (GOx), resulting in simultaneous CNT dispersion and facile enzyme immobilization through sequential enzyme adsorption, precipitation, and crosslinking (EAPC). The EAPC achieved high enzyme loading and stability because of crosslinked enzyme coatings on intact CNTs, while obviating the chemical pretreatment that can seriously damage the electron conductivity of CNTs...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28052671/role-of-organic-solvents-in-immobilizing-fungus-laccase-on-single-walled-carbon-nanotubes-for-improved-current-response-in-direct-bioelectrocatalysis
#14
Fei Wu, Lei Su, Ping Yu, Lanqun Mao
Improving bioelectrocatalytic current response of redox enzymes on electrodes has been a focus in the development of enzymatic biosensors and biofuel cells. Herein a mediatorless electroreduction of oxygen is effectively improved in terms of a remarkable enhancement by ca. 600% in maximum reductive current by simply adding 20% ethanol into laccase solution during its immobilization onto single-walled carbon nanotubes (SWCNTs). Conformation analysis by circular dichroism and attenuated total reflectance infrared spectroscopy demonstrate promoted laccase-SWCNTs contact by ethanol, thus leading to favorable enzyme orientation on SWCNTs...
January 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28050853/metagenomic-insights-into-the-rumen-microbial-fibrolytic-enzymes-in-indian-crossbred-cattle-fed-finger-millet-straw
#15
V Lyju Jose, Thulasi Appoothy, Ravi P More, A Sha Arun
The rumen is a unique natural habitat, exhibiting an unparalleled genetic resource of fibrolytic enzymes of microbial origin that degrade plant polysaccharides. The objectives of this study were to identify the principal plant cell wall-degrading enzymes and the taxonomic profile of rumen microbial communities that are associated with it. The cattle rumen microflora and the carbohydrate-active enzymes were functionally classified through a whole metagenomic sequencing approach. Analysis of the assembled sequences by the Carbohydrate-active enzyme analysis Toolkit identified the candidate genes encoding fibrolytic enzymes belonging to different classes of glycoside hydrolases(11,010 contigs), glycosyltransferases (6366 contigs), carbohydrate esterases (4945 contigs), carbohydrate-binding modules (1975 contigs), polysaccharide lyases (480 contigs), and auxiliary activities (115 contigs)...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28045587/conjugation-of-%C3%AF-bt1-derived-integrative-plasmid-pdzl802-in-amycolatopsis-mediterranei-u32
#16
Chen Li, Li Zhou, Ying Wang, Guoping Zhao, Xiaoming Ding
The genus Amycolatopsis is well known for its ability to produce antibiotics, and an increasing number of valuable biotechnological applications, such as bioremediation, biodegradation, bioconversion, and potentially biofuel, that employ this genus have been developed. Amycolatopsis mediterranei is an industrial-scale producer of the important antibiotic rifamycin, which plays a vital role in antimycobacterial therapy. Genetic studies of Amycolatopsis species have progressed slowly due to the lack of efficient transformation methods and stable plasmid vectors...
January 3, 2017: Bioengineered
https://www.readbyqxmd.com/read/28032192/biotechnological-exploitation-of-tetrapisispora-phaffii-killer-toxin-heterologous-production-in-komagataella-phaffii-pichia-pastoris
#17
Rossella Chessa, Sara Landolfo, Maurizio Ciani, Marilena Budroni, Severino Zara, Murat Ustun, Zeynep Petek Cakar, Ilaria Mannazzu
The use of natural antimicrobials from plants, animals and microorganisms to inhibit the growth of pathogenic and spoilage microorganisms is becoming more frequent. This parallels the increased consumer interest towards consumption of minimally processed food and 'greener' food and beverage additives. Among the natural antimicrobials of microbial origin, the killer toxin produced by the yeast Tetrapisispora phaffii, known as Kpkt, appears to be a promising natural antimicrobial agent. Kpkt is a glycoprotein with β-1,3-glucanase and killer activity, which induces ultrastructural modifications to the cell wall of yeast of the genera Kloeckera/Hanseniaspora and Zygosaccharomyces...
December 28, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28030766/boosted-sensor-performance-by-surface-modification-of-bifunctional-rht-type-metal-organic-framework-with-nanosized-electrochemically-reduced-graphene-oxide
#18
Cong Li, Tingting Zhang, Jingyu Zhao, He Liu, Bo Zheng, Yue Gu, Xiaoyi Yan, Yaru Li, Nannan Lu, Zhiquan Zhang, Guodong Feng
The surface and interface could be designed to enhance properties of electrocatalysts, and they are regarded as the key characteristics. This report describes surface modification of a bifunctional rht-type metal-organic framework (MOF, Cu-TDPAT) with nanosized electrochemically reduced graphene oxide (n-ERGO). The hybrid strategy results in a Cu-TDPAT-n-ERGO sensor with sensitive and selective response toward hydrogen peroxide (H2O2). Compared with Cu-TDPAT, Cu-TDPAT-n-ERGO exhibits significantly enhanced electrocatalytic activities, highlighting the importance of n-ERGO in boosting their electrocatalytic activity...
January 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28018484/mechanistic-insights-into-the-effect-of-imidazolium-ionic-liquid-on-lipid-production-by-geotrichum-fermentans
#19
Li-Ping Liu, Min-Hua Zong, Robert J Linhardt, Wen-Yong Lou, Ning Li, Chao Huang, Hong Wu
BACKGROUND: Ionic liquid (IL) pretreatment has emerged as a promising technique that enables complete utilization of lignocellulosic biomass for biofuel production. However, imidazolium IL has recently been shown to exhibit inhibitory effect on cell growth and product formation of industrial microbes, such as oleaginous microorganisms. To date, the mechanism of this inhibition remains largely unknown. RESULTS: In this study, the feasibility of [Bmim][OAc]-pretreated rice straw hydrolysate as a substrate for microbial lipid production by Geotrichum fermentans, also known as Trichosporon fermentans, was evaluated...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28018390/emerging-technologies-for-the-production-of-renewable-liquid-transport-fuels-from-biomass-sources-enriched-in-plant-cell-walls
#20
REVIEW
Hwei-Ting Tan, Kendall R Corbin, Geoffrey B Fincher
Plant cell walls are composed predominantly of cellulose, a range of non-cellulosic polysaccharides and lignin. The walls account for a large proportion not only of crop residues such as wheat straw and sugarcane bagasse, but also of residues of the timber industry and specialist grasses and other plants being grown specifically for biofuel production. The polysaccharide components of plant cell walls have long been recognized as an extraordinarily large source of fermentable sugars that might be used for the production of bioethanol and other renewable liquid transport fuels...
2016: Frontiers in Plant Science
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