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biofuel cell

Raphael Ferreira, Paulo Gonçalves Teixeira, Michael Gossing, Florian David, Verena Siewers, Jens Nielsen
Triacylglycerols (TAGs) are valuable versatile compounds that can be used as metabolites for nutrition and health, as well as feedstocks for biofuel production. Although Saccharomyces cerevisiae is the favored microbial cell factory for industrial production of biochemicals, it does not produce large amounts of lipids and TAGs comprise only ~1% of its cell dry weight. Here, we engineered S. cerevisiae to reorient its metabolism for overproduction of TAGs, by regulating lipid droplet associated-proteins involved in TAG synthesis and hydrolysis...
June 2018: Metabolic Engineering Communications
Quang Anh Tuan Le, Hee Gon Kim, Yong Hwan Kim
The electro-biocatalytic conversion of CO2 into formic acid using whole-cell and isolated biocatalysts is useful as an alternative route for CO2 sequestration. In this study, Shewanella oneidensis MR-1 (S. oneidensis MR-1), a facultative aerobic bacterium that has been extensively studied for its utility as biofuel cells as well as for the detoxification of heavy metal oxides (i.e., MnO2 , uranium), has been applied for the first time as a whole-cell biocatalyst for formic acid synthesis from gaseous CO2 and electrons supplied from an electrode...
September 2018: Enzyme and Microbial Technology
Alaa A Oughli, Marisela Vélez, James A Birrell, Wolfgang Schuhmann, Wolfgang Lubitz, Nicolas Plumeré, Olaf Rüdiger
In this work we present a viologen-modified electrode providing protection for hydrogenases against high potential inactivation. Hydrogenases, including O2-tolerant classes, suffer from reversible inactivation upon applying high potentials, which limits their use in biofuel cells to certain conditions. Our previously reported protection strategy based on the integration of hydrogenase into redox matrices enabled the use of these biocatalysts in biofuel cells even under anode limiting conditions. However, mediated catalysis required application of an overpotential to drive the reaction, and this translates into a power loss in a biofuel cell...
June 8, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Sandra Henritzi, Manuel Fischer, Martin Grininger, Mislav Oreb, Eckhard Boles
Background: The ideal biofuel should not only be a regenerative fuel from renewable feedstocks, but should also be compatible with the existing fuel distribution infrastructure and with normal car engines. As the so-called drop-in biofuel, the fatty alcohol 1-octanol has been described as a valuable substitute for diesel and jet fuels and has already been produced fermentatively from sugars in small amounts with engineered bacteria via reduction of thioesterase-mediated premature release of octanoic acid from fatty acid synthase or via a reversal of the β-oxidation pathway...
2018: Biotechnology for Biofuels
Chen-Jung Lee, Huifang Wang, Megan Young, Shengxi Li, Fang Cheng, Hongbo Cong, Gang Cheng
Poly(3,4-ethylenedioxythiophene) (PEDOT) has been widely studied in recent decades due to its high stability, biocompatibility, low redox potential, moderate band gap, and optical transparency in its conducting state. However, for its long-term in vivo applications, the biocompatibility of PEDOT still need to be improved. To address this challenge, zwitterionic Poly(sulfobetaine 3,4-ethylenedioxythiophene) (PSBEDOT) that contains EDOT backbone with sulfobetaine functional side chains were developed in our previous study...
June 4, 2018: Acta Biomaterialia
Kiyan Shabestary, Josefine Anfelt, Emil Ljungqvist, Michael Jahn, Lun Yao, Elton P Hudson
Photoautotrophic production of fuels and chemicals by cyanobacteria typically gives lower volumetric productivities and titers than heterotrophic production. Cyanobacteria cultures become light limited above an optimal cell density, so that this substrate is not supplied to all cells sufficiently. Here, we investigate genetic strategies for a two-phase cultivation, where biofuel-producing Synechocystis cultures are limited to an optimal cell density through inducible CRISPR interference (CRISPRi) repression of cell growth...
June 8, 2018: ACS Synthetic Biology
Charnete Casimero, Aaron McConville, John-Joe Fearon, Clare L Lawrence, Charlotte M Taylor, Robert B Smith, James Davis
Monitoring pH within microbial reactors has become an important requirement across a host of applications ranging from the production of functional foods (probiotics) to biofuel cell systems. An inexpensive and scalable composite sensor capable of monitoring the pH within the demanding environments posed by microbial reactors has been developed. A custom designed flavin derivative bearing an electropolymerisable phenol monomer was used to create a redox film sensitive to pH but free from the interferences that can impede conventional pH systems...
October 16, 2018: Analytica Chimica Acta
S Angioni, L Millia, P Mustarelli, E Doria, M E Temporiti, B Mannucci, F Corana, E Quartarone
Here, we demonstrate a very efficient simultaneous approach of bioenergy generation from wastewater and added-value compounds production by using a photosynthetic microalgae microbial fuel cells (PMFC), based on polybenzimidazole (PBI) composite membrane as separator. The use of PBI was proved to be very promising, even more convenient than Nafion™ in terms of energy performances as well as cost and sustainability. This polymer is also easily autoclavable, so allowing a re-use of the separator with a consequent beneficial cost effect...
March 2018: Heliyon
Fortunate Laker, Arnold Agaba, Andrew Akatukunda, Robert Gazet, Joshua Barasa, Sarah Nanyonga, Deborah Wendiro, Alex Paul Wacoo
The overwhelming demand of oil and fats to meet the ever increasing needs for biofuel, cosmetics production, and other industrial purposes has enhanced a number of innovations in this industry. One such innovation is the use of microorganisms as alternative sources of oil and fats. Organic solid waste that is causing a big challenge of disposal worldwide is biodegradable and can be utilized as substrate for alternative oil production. The study evaluated the potential of isolated yeast-like colonies to grow and accumulate oil by using organic solid waste as substrate...
2018: Journal of Lipids
Erik K R Hanko, Charles M Denby, Violeta Sànchez I Nogué, Weiyin Lin, Kelsey J Ramirez, Christine A Singer, Gregg T Beckham, Jay D Keasling
Medium- and long-chain methyl ketones are fatty acid-derived compounds that can be used as biofuel blending agents, flavors and fragrances. However, their large-scale production from sustainable feedstocks is currently limited due to the lack of robust microbial biocatalysts. The oleaginous yeast Yarrowia lipolytica is a promising biorefinery platform strain for the production of methyl ketones from renewable lignocellulosic biomass due to its natively high flux towards fatty acid biosynthesis. In this study, we report the metabolic engineering of Y...
May 28, 2018: Metabolic Engineering
Takanori Maeno, Takanori Uzawa, Izumi Kono, Kazunori Okano, Takanori Iino, Keisuke Fukita, Yuki Oshikawa, Taro Ogawa, Osamu Iwata, Takuro Ito, Kengo Suzuki, Keisuke Goda, Yoichiroh Hosokawa
Microalgae-based metabolic engineering has been proven effective for producing valuable substances such as food supplements, pharmaceutical drugs, biodegradable plastics, and biofuels in the past decade. The ability to accurately visualize and quantify intracellular metabolites in live microalgae is essential for efficient metabolic engineering, but remains a major challenge due to the lack of characterization methods. Here we demonstrate it by synthesizing fluorogenic peptide aptamers with specific binding affinity to a target metabolite and delivering them into live microalgae by femtosecond laser photoporation at single-cell resolution...
May 29, 2018: Scientific Reports
John E Hallsworth
Stress is an inextricable aspect of life, and stress biology has been a field of intensive study over the last 200-300 years. In human psychology, we consider a stress-free condition to be one of relaxation or happiness, yet with respect to microbial cells we do not have a concept that describes being non-stressed. Stresses within, and stress tolerance of, microbial systems lie at the crux of critical global challenges, such as optimising soil- and plant-health and crop yields; reducing food spoilage; bioremediation of polluted environments; effective biological control and biofuel production; gaining insight into aging processes in humans; and understanding astrobiology...
June 2018: Fungal Biology
Janwa El-Maiss, Marco Cuccarese, Clément Maerten, Paolo Lupattelli, Lucia Chiummiento, Maria Funicello, Pierre Schaaf, Loïc Jierry, Fouzia Boulmedais
In medical diagnosis and environmental monitoring, enzymatic biosensors are widely applied because of their high sensitivity, potential selectivity, and their possibility of miniaturization/automation. Enzyme immobilization is a critical process in the development of this type of biosensors with the necessity to avoid the denaturation of the enzymes and ensuring their accessibility toward the analyte. Electrodeposition of macromolecules is increasingly considered to be the most suitable method for the design of biosensors...
May 25, 2018: ACS Applied Materials & Interfaces
Ahmed Al-Kattan, Viraj P Nirwan, Anton Popov, Yury V Ryabchikov, Gleb Tselikov, Marc Sentis, Amir Fahmi, Andrei V Kabashin
Driven by surface cleanness and unique physical, optical and chemical properties, bare (ligand-free) laser-synthesized nanoparticles (NPs) are now in the focus of interest as promising materials for the development of advanced biomedical platforms related to biosensing, bioimaging and therapeutic drug delivery. We recently achieved significant progress in the synthesis of bare gold (Au) and silicon (Si) NPs and their testing in biomedical tasks, including cancer imaging and therapy, biofuel cells, etc. We also showed that these nanomaterials can be excellent candidates for tissue engineering applications...
May 24, 2018: International Journal of Molecular Sciences
Lei Qin, Lu Liu, Zhongming Wang, Weining Chen, Dong Wei
To determine the feasibility of microalgae-yeast mixed culture using the liquid digestate of dairy wastewater (LDDW) for biofuels and single cell protein (SCP) production, the cell growth, nutrient removal and outputs evaluation of the mono and mixed culture of Chlorella vulgaris and Yarrowia lipolytica in LDDW were investigated by adding glycerol as carbon source. The results showed that the mixed culture could enhance the biological utilization efficiency of nitrogen and phosphorus, and obtain higher yield of biomass (1...
May 18, 2018: Bioresource Technology
Qun Lin, Wen-Hao Zhuo, Xin-Wei Wang, Chang-Ping Chen, Ya-Hui Gao, Jun-Rong Liang
Microalgae are considered as attractive feedstocks for biofuel production nowadays because of their high lipid contents and easy cultivation. In the present study, two diatoms, Thalassiosira weissflogii and Chaetoceros muelleri, were cultured under various nutrient-limitation conditions to explore their comprehensive lipid accumulation profiles for further commercialization. In T. weissflogii, the highest neutral lipid accumulation and highest lipid productivity (14.28 mg L-1  day-1 ) were both recorded under P-limitation...
May 22, 2018: Bioprocess and Biosystems Engineering
Andreza de Mello Lopes, Edivaldo Ximenes Ferreira Filho, Leonora Rios de Souza Moreira
Alternative energy sources have received increasing attention in recent years. The possibility of adding value to agricultural wastes, by producing biofuels and other products with economic value from lignocellulosic biomass by enzymatic hydrolysis, has been widely explored. Lignocellulosic biomass, as well as being an abundant residue, is a complex recalcitrant structure that requires a consortium of enzymes for its complete degradation. Pools of enzymes with different specificities acting together usually produce an increase in hydrolysis yield...
May 22, 2018: Journal of Applied Microbiology
Chaofan Zhang, Qingcheng Li, Liang Fu, Dandan Zhou, John C Crittenden
Cultivating microalgae using wastewater is an economical strategy to produce biofuel; however, microbial contamination has to be controlled strictly. Microalgae lipid accumulation can be triggered by environmental pressures, and here, we studied whether microbial contamination is the pressure for microalgae. We hypothesized this pressure was forced via cell-to-cell communication with quorum sensing molecules (QSMs). In this work, we verified the impacts of QSMs produced by activated sludge (wastewater-born microbial consortiums) on both lipid content and biomass production of the microalgae Chlorophyta sp...
May 18, 2018: Bioresource Technology
Yolina Hubenova, Rumyana Bakalska, Mario Mitov
Modification of carbonaceous materials with different conductive coatings is a successful approach to enhance their electrocatalytic activity and thus to increase the electrical outputs when used as electrodes in biofuel cells. In this study, a methodology for electrodeposition of styrylquinolinium dye on carbon felt was developed. The produced dye electrodeposits were characterized by means of AFM, ESI-MS/MS and NMR spectroscopy. The obtained data reveal that the dye forms overlaid layers consisting of monomer molecules most likely with an antiparallel orientation...
May 10, 2018: Bioelectrochemistry
Han Sun, Xuemei Mao, Tao Wu, Yuanyuan Ren, Feng Chen, Bin Liu
Revenues of carotenoid and lipid biosynthesis under excess light and nitrogen starvation were firstly analyzed for the increased biomass value through carbon metabolism analysis. The results suggested excess light and nitrogen starvation resulted in carbon partitioning among protein, starch, lipid and carotenoid. Nitrogen starvation promoted more cellular lipid content than excess light, while excess light promoted carotenoid and polyunsaturated fatty acid accumulation. In the molecular level, the stresses redirected carbon skeletons into the central metabolite of pyruvate and oriented into starch and lipid as the primary and secondary carbon storage, respectively...
May 10, 2018: Bioresource Technology
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