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Transglutaminase 2

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https://www.readbyqxmd.com/read/28810589/phenotypic-switch-in-lung-interstitial-macrophage-polarization-in-an-ovalbumin-induced-mouse-model-of-asthma
#1
Hanxiang Nie, Ailing Wang, Qing He, Qiaoyu Yang, Linlin Liu, Guqin Zhang, Yi Huang, Xuhong Ding, Hongying Yu, Suping Hu
Macrophage phenotype and function varies according to their polarized state, which in turn is dependent on microenvironmental stimuli. Under normal physiological conditions, lung interstitial macrophages that express interleukin (IL)-10 are considered to serve regulatory roles in the prevention of allergic reactions in the airways. However, the phenotypic profile of lung interstitial macrophages during the pathophysiology of asthma remains unknown. In the current study, the phenotypic characteristics of lung interstitial macrophages were investigated in an ovalbumin (OVA)-induced mouse model of asthma...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28810543/atorvastatin-has-a-protective-effect-in-a-mouse-model-of-bronchial-asthma-through-regulating-tissue-transglutaminase-and-triggering-receptor-expressed-on-myeloid-cells-1-expression
#2
Ming-Wei Liu, Rong Liu, Hai-Ying Wu, Mei Chen, Min-Na Dong, Yun-Qiao Huang, Chun-Hai Zhang, Yin-Zhong Wang, Jing Xia, Yang Shi, Feng-Mei Xie, Hua Luo, Xin-Yuan Zhao, Wei Wei, Mei-Xian Su
Airway remodeling in asthma contributes to airway hyperreactivity, loss of lung function and persistent symptoms. Current therapies do not adequately treat the structural airway changes associated with asthma. Statin drugs have improved respiratory health and their therapeutic potential in asthma has been tested in clinical trials. However, the mechanism of action of statins in this context has remained elusive. The present study hypothesized that atorvastatin treatment of ovalbumin-exposed mice attenuates early features of airway remodeling via a mevalonate-dependent mechanism...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28806297/adherence-to-a-gluten-free-diet-assessment-by-dietician-interview-and-serology
#3
Pooja Mehta, Zhaoxing Pan, Marisa Dzarnoski Riley, Edwin Liu
We aimed to 1) determine if tissue transglutaminase autoantibody (tTG) positivity was associated with dietitian-assessed adherence to a gluten-free diet in pediatric patients with celiac disease and 2) identify areas where adherence falters. We reviewed the records of children with celiac disease who had a standardized evaluation of adherence by a registered dietitian. A negative tTG value was not associated with good adherence (p = NS). Adherent and nonadherent children differed with respect to purposeful and accidental gluten exposure (p < 0...
August 11, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28782118/undetectable-negative-tissue-transglutaminase-iga-antibodies-predict-mucosal-healing-in-treated-coeliac-disease-patients
#4
H Fang, K S King, J J Larson, M R Snyder, T T Wu, M J Gandhi, J A Murray
BACKGROUND: Tissue transglutaminase (tTG) immunoglobulin A (IgA) testing is a sensitive adjunct to the diagnosis of coeliac disease. The threshold for positivity was developed for diagnosis, with negative results reported as below the reference value (<4 U/mL). AIM: To investigate if an undetectable (tTG IgA<1.2 U/mL) is more predictive of healing compared to patients with negative but detectable serology (1.2-3.9 U/mL). METHODS: We performed a retrospective study of 402 treated coeliac disease patients seen at the Mayo Clinic with negative tTG IgA values drawn within 1 month of duodenal biopsy between January 2009 and December 2015...
August 7, 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28781956/the-proteomic-profile-of-a-mouse-model-of-proliferative-vitreoretinopathy
#5
Bernadett Márkus, Zsuzsanna Pató, Zsolt Sarang, Réka Albert, József Tőzsér, Goran Petrovski, Éva Csősz
Proliferative vitreoretinopathy (PVR) develops as a complication of retinal detachment surgery and represents a devastating condition leading to serious vision loss. A good animal model that permits extensive functional studies and drug testing is crucial in finding better therapeutic modalities for PVR. A previously established mouse model, using dispase injection, was analyzed from the proteomic point of view, examining global protein profile changes by 2D electrophoresis, image analysis and HPLC-tandem mass spectrometry-based protein identification...
August 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28775095/transglutaminase-2-in-pulmonary-and-cardiac-tissue-remodeling-in-experimental-pulmonary-hypertension
#6
Krishna C Penumatsa, Deniz Toksoz, Rod R Warburton, Mousa Kharnaf, Ioana R Preston, Navin Kumar Kapur, Chaitan Khosla, Nicholas S Hill, Barry L Fanburg
Tissue matrix remodeling and fibrosis leading to loss of pulmonary arterial and right ventricular compliance are important features of both experimental and clinical pulmonary hypertension (PH). We have previously reported that transglutaminase 2 (TG2) is involved in PH development while others have shown it to be a crosslinking enzyme that participates in remodeling of extracellular matrix in fibrotic diseases in general. In the present studies, we used a mouse model of experimental PH (Sugen 5416 and hypoxia; SuHypoxia) and cultured primary human cardiac and pulmonary artery adventitial fibroblasts to evaluate the relationship of TG2 to the processes of fibrosis, protein crosslinking, extracellular matrix collagen accumulation and fibroblast to myofibroblast transformation...
August 3, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28774822/browning-deficiency-and-low-mobilization-of-fatty-acids-in-gonadal-white-adipose-tissue-leads-to-decreased-cold-tolerance-of-transglutaminase-2-knock-out-mice
#7
András Mádi, Ixchelt Cuaranta-Monroy, Kinga Lénárt, Attila Pap, Zoltán András Mezei, Endre Kristóf, Anna Oláh, György Vámosi, Zsolt Bacsó, Péter Bai, László Fésüs
During cold-exposure 'beige' adipocytes with increased mitochondrial content are activated in white adipose tissue (WAT). These cells, similarly to brown adipocytes (BAT), dissipate stored chemical energy in the form of heat with the help of uncoupling protein 1 (UCP1). We investigated the effect of TG2 ablation on the function of ATs in mice. Although TG2(+/+) and TG2(-/-) mice had the same amount of WAT and BAT, we found that TG2(+/+) animals could tolerate acute cold exposure for 4h, whereas TG2(-/-) mice only for 3h...
July 31, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28767399/effect-of-p53-on-pancreatic-cancer-glucose-tolerance-abnormalities-by-regulating-transglutaminase-2-in-resistance-to-glucose-metabolic-stress
#8
Xiao Su, Xiangyi He, Qiwen Ben, Weiyi Wang, Huan Song, Qiao Ye, Yi Zang, Weiguang Li, Ping Chen, Weiyan Yao, Yaozong Yuan
Pancreatic ductal adenocarcinoma (PanCa) is an extremely lethal disease characterized by mutations of p53 in up to 70% of cases. Our previous studies have confirmed that hyperglycemia may be the first clinical manifestation for the early diagnosis of PanCa. In this article, we showed that targeted knockdown of TG2 or p53 in tumor cells led to decreased cell survival in response to glucose deprivation, while this phenomenon was abolished by combined inhibition of TG2 and p53. We observed that inhibition of TG2 or p53 sensitized glucose deprivation resistance through an intracellular reactive oxygen species (ROS) pathway and the induction of Bcl-2...
July 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28756478/protein-19-f-labeling-using-transglutaminase-for-the-nmr-study-of-intermolecular-interactions
#9
Yoshikazu Hattori, David Heidenreich, Yuki Ono, Toshihiko Sugiki, Kei-Ichi Yokoyama, Ei-Ichiro Suzuki, Toshimichi Fujiwara, Chojiro Kojima
The preparation of stable isotope-labeled proteins is important for NMR studies, however, it is often hampered in the case of eukaryotic proteins which are not readily expressed in Escherichia coli. Such proteins are often conveniently investigated following post-expression chemical isotope tagging. Enzymatic (15)N-labeling of glutamine side chains using transglutaminase (TGase) has been applied to several proteins for NMR studies. (19)F-labeling is useful for interaction studies due to its high NMR sensitivity and susceptibility...
July 29, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28754668/transglutaminase-2-inhibition-reverses-mesenchymal-transdifferentiation-of-glioma-stem-cells-by-regulating-c-ebp%C3%AE-signaling
#10
Jinlong Yin, Young Taek Oh, Jeong-Yub Kim, Sung Soo Kim, Eunji Choi, Tae Hoon Kim, Jun Hee Hong, Nakho Chang, Hee Jin Cho, Jason Kyungha Sa, Jeong Cheol Kim, Hyung Joon Kwon, Saewhan Park, Weiwei Lin, Ichiro Nakano, Ho-Shin Gwak, Heon Yoo, Seung-Hoon Lee, Jeongwu Lee, Jong Heon Kim, Soo-Youl Kim, Do-Hyun Nam, Myung-Jin Park, Jong Bae Park
Necrosis is a hallmark of glioblastoma (GBM) and is responsible for poor prognosis and resistance to conventional therapies. However, the molecular mechanisms underlying necrotic microenvironment-induced malignancy of GBM have not been elucidated. Here, we report that transglutaminase 2 (TGM2) is upregulated in the perinecrotic region of GBM and triggered mesenchymal (MES) transdifferentiation of glioma stem cells (GSC) by regulating master transcription factors (TF), such as C/EBPβ, TAZ, and STAT3. TGM2 expression was induced by macrophages/microglia-derived cytokines via NFκB activation and further degraded DNA damage-inducible transcript 3 (GADD153) to induce C/EBPβ expression, resulting in expression of the MES transcriptome...
July 28, 2017: Cancer Research
https://www.readbyqxmd.com/read/28754379/%C3%AE-2-adrenoceptor-induced-modulation-of-transglutaminase-2-transamidase-activity-in-cardiomyoblasts
#11
Falguni S Vyas, Carl P Nelson, Fiona Freeman, David J Boocock, Alan J Hargreaves, John M Dickenson
Tissue transglutaminase 2 (TG2) is modulated by protein kinase A (PKA) mediated phosphorylation: however, the precise mechanism(s) of its modulation by G-protein coupled receptors coupled to PKA activation are not fully understood. In the current study we investigated the potential regulation of TG2 activity by the β2-adrenoceptor in rat H9c2 cardiomyoblasts. Transglutaminase transamidation activity was assessed using amine-incorporating and protein cross-linking assays. TG2 phosphorylation was determined via immunoprecipitation and Western blotting...
July 25, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28753180/mass-screening-for-celiac-disease-among-school-aged-children-toward-exploring-celiac-iceberg-in-saudi-arabia
#12
Abdulrahman Al-Hussaini, Riccardo Troncone, Musa Khormi, Muath AlTuraiki, Wahid Alkhamis, Mona Alrajhi, Thana Halal, Mosa Fagih, Sahar Alharbi, Muhammed Salman Bashir, Aziz Elchentoufi
OBJECTIVES: We conducted this mass screening study to determine the prevalence of celiac disease (CD) and characterize the celiac iceberg among Saudi pediatric population in Riyadh, the capital city of Saudi Arabia. METHODS: Over the study period (January 2014 to June 2016), we have conducted a cross-sectional, mass screening, IgA-tissue transglutaminase (TTG-IgA)-based study on 7930 Saudi students from primary and intermediate schools in Riyadh. Students with positive TTG-IgA (> 20 U/L) were called in the hospital to undergo a repeat of TTG-IgA; in those with borderline positive TTG-IgA (20-60 U/L) IgA-endomyseal antibody (EMA-IgA) test was performed...
July 27, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28749395/comparative-study-of-clinical-features-of-patients-with-celiac-disease-those-with-concurrent-celiac-disease-type-1-diabetes-mellitus
#13
Sanjay Kumar Bhadada, Ashu Rastogi, Aakash Agarwal, Rashi Kochhar, Rakesh Kochhar, Anil Bhansali
BACKGROUND & OBJECTIVES: Celiac disease (CD) and type 1 diabetes mellitus (T1DM) share a common genetic locus and clinical manifestations. The present study was planned to compare clinical, biochemical and hormonal profiles of patients with CD and CD with T1DM. METHODS: Records of CD patients with age ≤20 yr, available anthropometric measurements, haematological, biochemical and hormonal workup with tissue transglutaminase IgA antibody and duodenal biopsy (Marsh grade) were screened...
March 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/28747673/engineered-isopeptide-bond-stabilized-fibrin-inspired-nanoscale-peptide-based-sealants-for-efficient-blood-clotting
#14
Snehasish Ghosh, Sanchita Mukherjee, Chiranjit Dutta, Kasturee Chakraborty, Paramita Gayen, Somnath Jan, Dhananjay Bhattacharyya, Rituparna Sinha Roy
Designing biologically inspired nanoscale molecular assembly with desired functionality is a challenging endeavour. Here we report the designing of fibrin-inspired nanostructured peptide based sealants which facilitate remarkably fast entrapping of blood corpuscles (~28 seconds) in contrast to fibrin (~56 seconds). Our engineered sealants are stabilized by lysine-aspartate ionic interactions and also by N(ε)(γ-glutamyl) lysine isopeptide bond mediated covalent interaction. Each sealant is formed by two peptides having complementary charges to promote lysine-aspartate ionic interactions and designed isopeptide bond mediated interactions...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28745034/transglutaminase-antibodies-and-celiac-disease-in-children-with-type-1-diabetes-and-in-their-family-members
#15
Anna Parkkola, Taina Härkönen, Samppa J Ryhänen, Raivo Uibo, Jorma Ilonen, Mikael Knip
OBJECTIVES: We set out to determine the prevalence of tissue transglutaminase antibodies (anti-tTG) and celiac disease (CD) in children with newly diagnosed type 1 diabetes (T1D) and their first-degree relatives (FDR). The hypothesis was that the individuals with both diabetes and CD form a distinct subgroup in terms of human leukocyte antigen (HLA) class II genetics, islet autoantibodies, and clinical characteristics at diabetes diagnosis. SUBJECTS AND METHODS: This population-based observational study included 745 index children with T1D and their 2692 FDR from the Finnish Pediatric Diabetes Register...
July 26, 2017: Pediatric Diabetes
https://www.readbyqxmd.com/read/28739407/ultrasensitive-transglutaminase-based-nanosensor-for-early-detection-of-celiac-disease-in-human
#16
Shagun Gupta, Ankur Kaushal, Ashok Kumar, Dinesh Kumar
A GQD/PAMAM nanohybrid modified on gold nanoparticles (AuNP) embedded in multiwalled carbon nanotubes (MWCNT) based immunosensor was developed for detection of celiac disease in human. The AuNP embedded in MWCNT were linked with thiol groups of cysteine and amino groups were further coupled with carboxyl moieties of graphene quantum dots (GQDs) using EDC-NHS cross-linking chemistry. To enhance the sensitivity of the sensor, polyamidoamine (PAMAM) dendrimer was attached with GQDs through carbodiimide coupling...
July 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28737923/cosilencing-intestinal-transglutaminase-2-and-interleukin-15-using-gelatin-based-nanoparticles-in-an-in-vitro-model-of-celiac-disease
#17
Husain Attarwala, Valerie Clausen, Prasoon Chaturvedi, Mansoor M Amiji
In this study, we have developed a type B gelatin nanoparticle based siRNA delivery system for silencing of intestinal transglutaminase-2 (TG2) and interleukin-15 (IL-15) genes in cultured human intestinal epithelial cells (Caco-2) and murine alveolar macrophage cells (J774A.1). Small interfering RNA (siRNA) targeting the TG2 or IL-15 gene was encapsulated within gelatin nanoparticles using ethanol-water solvent displacement method. Size, charge, and morphology of gelatin nanoparticles were evaluated using a Zetasizer instrument and transmission electron microscopy...
August 10, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28736752/inhibitory-activity-of-a-concanavalin-isolated-fraction-from-a-glucosamine-peptides-reaction-system-against-heat-resistant-e-coli
#18
Yuliya Hrynets, Daylin Johana Hincapie Martinez, Maurice Ndagijimana, Mirko Betti
Alcalase-derived gelatin hydrolysates were glycated with glucosamine in the presence (+) or absence (-) of transglutaminase (TGase), and their antimicrobial activities toward Escherichia coli AW 1.7 were studied. Glycation treatments were subjected to concanavalin A affinity chromatography to selectively collect the glycopeptide-enriched fractions and the changes in antimicrobial activity were determined. The minimum inhibitory concentration of glycated hydrolysates decreased by 1.2 times compared to the native hydrolysate, with no differences between (+) or (-) TGase treatments...
July 2017: Heliyon
https://www.readbyqxmd.com/read/28732930/transglutaminase-set-colloidal-properties-of-wheat-gluten-with-ultrasound-pretreatments
#19
Xin-Sheng Qin, Qiao-Qiao Sun, Yan-Yan Zhao, Xi-Yang Zhong, Dong-Dong Mu, Shao-Tong Jiang, Shui-Zhong Luo, Zhi Zheng
The low solubility of wheat gluten limits its accessibility. This work aimed to study the impact of ultrasonic pretreatments on the gelation of wheat gluten. The pretreatments included ultrasound combined with alkali, urea, Na2SO3, with or without the addition of transglutaminase (TGase). The gel strength of wheat gluten was 287g/cm(2) after treatment with Na2SO3/ultrasound/TGase. The free sulfhydryl and disulfide bond content was significantly affected by ultrasound treatment. After treatments including TGase crosslinking, the molecular weight of wheat gluten complexes became larger...
November 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28730262/crypt-hyperplastic-enteropathy-in-distal-duodenum-in-helicobacter-pylori-infection-report-of-two-cases-without-evidence-of-celiac-disease
#20
Mariana Jinga, Daniel Vasile Balaban, Ileana Peride, Andrei Niculae, Irina Monica DuŢescu, Florina Vasilescu, Markku Mäki, Alina Mihaela Popp
OBJECTIVES: Patients with Helicobacter pylori (HP) infection have been reported to have in addition to duodenitis architectural changes of the duodenal bulb mucosa including villous atrophy and crypt hyperplasia. We here for the first time present two cases of HP-infected adult patients with a crypt hyperplastic enteropathy also in the distal duodenum mimicking celiac disease (CD). METHODS: We evaluated separately the morphology of the anatomical bulb and distal duodenal mucosa using validated quantitative morphometric tools, i...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
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