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https://www.readbyqxmd.com/read/29788452/glutamine-alleviates-heat-stress-induced-impairment-of-intestinal-morphology-intestinal-inflammatory-response-and-barrier-integrity-in-broilers
#1
Q J Wu, N Liu, X H Wu, G Y Wang, L Lin
The aim of this study was to investigate the protective effect of glutamine (Gln) on the intestinal morphology, intestinal inflammatory response, and barrier integrity in broilers exposed to high ambient temperature. Three-hundred-sixty 21-d-old Arbor Acres broilers (half male and half female) were randomly allocated to 4 treatment groups in a completely randomized design, each of which included 6 replicates with 15 birds per replicate, for 21 d. The 4 treatment groups were as follows: the control group, in which birds were kept in a thermoneutral room at 22 ± 1°C (no stress, NS; fed a basal diet); the heat stress group (36 ± 1°C for 10 h/d from 08:00 to 18:00 h and 22 ± 1°C for the remaining time, heat stress (HT); fed a basal diet); and heat stress + Gln group (0...
May 17, 2018: Poultry Science
https://www.readbyqxmd.com/read/29785692/impact-of-enteral-nutrition-with-an-immunomodulating-diet-enriched-with-hydrolyzed-whey-peptide-on-infection-after-liver-transplantation
#2
Naoko Kamo, Toshimi Kaido, Yuhei Hamaguchi, Ryuji Uozumi, Shinya Okumura, Atsushi Kobayashi, Hisaya Shirai, Shintaro Yagi, Hideaki Okajima, Shinji Uemoto
BACKGROUND: Infection is a leading cause of death after liver transplantation (LT). Therefore, prevention of infection is crucial for improving outcomes after LT. We examined the impact of early enteral nutrition with an immunomodulating diet (IMD) enriched with hydrolyzed whey peptide (HWP) formulation on infection after living donor LT (LDLT), focusing on sarcopenia. METHODS: This study enrolled 279 consecutive patients who underwent primary LDLT at our institute between January 2008 and April 2015...
May 21, 2018: World Journal of Surgery
https://www.readbyqxmd.com/read/29784925/acrylamide-acute-neurotoxicity-in-adult-zebrafish
#3
Melissa Faria, Tamar Ziv, Cristian Gómez-Canela, Shani Ben-Lulu, Eva Prats, Karen Adriana Novoa-Luna, Arie Admon, Benjamin Piña, Romà Tauler, Leobardo Manuel Gómez-Oliván, Demetrio Raldúa
Acute exposure to acrylamide (ACR), a type-2 alkene, may lead to a ataxia, skeletal muscles weakness and numbness of the extremities in human and laboratory animals. In the present manuscript, ACR acute neurotoxicity has been characterized in adult zebrafish, a vertebrate model increasingly used in human neuropharmacology and toxicology research. At behavioral level, ACR-treated animals exhibited "depression-like" phenotype comorbid with anxiety behavior. At transcriptional level, ACR induced down-regulation of regeneration-associated genes and up-regulation of oligodendrocytes and reactive astrocytes markers, altering also the expression of genes involved in the presynaptic vesicle cycling...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784627/patient-friendly-summary-of-the-acr-appropriateness-criteria-ct-for-adult-minor-head-trauma
#4
Celena Romero, Ryan Lee
No abstract text is available yet for this article.
May 18, 2018: Journal of the American College of Radiology: JACR
https://www.readbyqxmd.com/read/29782701/tgf-%C3%AE-1-modified-hyaluronic-acid-poly-glycidol-hydrogels-for-chondrogenic-differentiation-of-human-mesenchymal-stromal-cells
#5
Thomas Böck, Verena Schill, Martin Krähnke, Andre F Steinert, Jörg Tessmar, Torsten Blunk, Jürgen Groll
In cartilage regeneration, the biomimetic functionalization of hydrogels with growth factors is a promising approach to improve the in vivo performance and furthermore the clinical potential of these materials. In order to achieve this without compromising network properties, multifunctional linear poly(glycidol) acrylate (PG-Acr) is synthesized and utilized as crosslinker for hydrogel formation with thiol-functionalized hyaluronic acid via Michael-type addition. As proof-of-principle for a bioactivation, transforming growth factor-beta 1 (TGF-β1) is covalently bound to PG-Acr via Traut's reagent which does not compromise the hydrogel gelation and swelling behavior...
May 21, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29780386/automated-processing-and-evaluation-of-anti-nuclear-antibody-indirect-immunofluorescence-testing
#6
Vincent Ricchiuti, Joseph Adams, Donna J Hardy, Alexander Katayev, James K Fleming
Indirect immunofluorescence (IIF) is considered by the American College of Rheumatology (ACR) and the international consensus on ANA patterns (ICAP) the gold standard for the screening of anti-nuclear antibodies (ANA). As conventional IIF is labor intensive, time-consuming, subjective, and poorly standardized, there have been ongoing efforts to improve the standardization of reagents and to develop automated platforms for assay incubation, microscopy, and evaluation. In this study, the workflow and performance characteristics of a fully automated ANA IIF system (Sprinter XL, EUROPattern Suite, IFA 40: HEp-20-10 cells) were compared to a manual approach using visual microscopy with a filter device for single-well titration and to technologist reading...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29779026/uromodulin-mrna-from-urinary-extracellular-vesicles-correlate-to-kidney-function-decline-in-type-2-diabetes-mellitus
#7
Cindy M Yamamoto, Taku Murakami, Melanie L Oakes, Masato Mitsuhashi, Colleen Kelly, Robert R Henry, Kumar Sharma
BACKGROUND: Extracellular vesicles (EVs) enclose mRNA derived from their cell of origin and are considered a source of potential biomarkers. We examined urinary EV mRNA from individuals with diabetic kidney disease (DKD), chronic kidney disease, type 2 diabetes (T2DM), and obese and healthy controls to determine if such biomarkers had the potential to classify kidney disease and predict patients at higher risk of renal function decline. METHODS: A total of 242 participants enrolled in this study...
May 18, 2018: American Journal of Nephrology
https://www.readbyqxmd.com/read/29778923/quantitative-proteomic-profiling-indicates-the-difference-in-reproductive-efficiency-between-meishan-and-duroc-boar-spermatozoa
#8
Li Xinhong, Linqing Zhen, Jieli Fu, Lirui Wang, Qiangzhen Yang, Peifei Li, Yuhua Li
The reproductive efficiency of Meishan pigs is higher than that of Duroc pigs, but the underlying molecular mechanism for this disparity remains unclear. No systematic quantitative proteomics studies, comparing global proteins in Meishan and Duroc boar spermatozoa have been reported. Therefore, we applied iTRAQ labeling coupled with mass spectrometry, and analyzed the differences in proteins between Meishan and Duroc sperm. In the present study, a total of 1597 proteins were quantified. Of these proteins, 190 showed statistically significant fold changes between Meishan and Duroc spermatozoa...
April 30, 2018: Theriogenology
https://www.readbyqxmd.com/read/29776866/hearing-impairment-in-type-2-diabetics-and-patients-with-early-diabetic-nephropathy
#9
Jianmin Ren, Fufu Ma, Yujing Zhou, Anting Xu, Jianjian Zhang, Rong Ma, Xiaoyan Xiao
AIMS: The study was to investigate the hearing function in subjects with non-diabetic nephropathy and diabetic nephropathy and analyze related clinical indexes of hearing impairment. METHODS: We assessed the hearing function of 30 diabetics (DM group), 30 patients with early diabetic nephropathy (DN group) and 30 healthy subjects (NC group) using pure-tone audiometry, otoacoustic emissions, electronystagmography, caloric test and cervical vestibular evoked myogenic potential (VEMP)...
April 5, 2018: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/29774602/pancreas-transplantation-using-compatible-but-non-identical-abo-blood-group-donors
#10
Santosh Nagaraju, Richard S Mangus, John A Powelson, Jonathan A Fridell
METHODS: A review of all pancreas transplants from a single institution from 01/2003 to 07/2016 (n=606) revealed 41 recipients of a NIC donor pancreas which were matched for age, race, gender, year and type of transplant with 41 ABO identical cases. Groups were compared for allograft survival, incidence of acute cellular rejection (ACR), length of hospital stay, 3-month readmissions and transfusion requirements. Serum haptoglobin and Lactate dehydrogenase were used to identify hemolysis in patients requiring repeated transfusions without overt blood loss...
May 17, 2018: Clinical Transplantation
https://www.readbyqxmd.com/read/29773274/scleroderma-in-children
#11
REVIEW
Francesco Zulian
Juvenile scleroderma with its two varieties, juvenile localized scleroderma and systemic sclerosis (SSc), represents the third most frequent rheumatic disease in childhood. In juvenile SSc, new developments have been recently reported in the fields of classification and monitoring. The introduction of new classification criteria for adult SSc has stimulated new ideas on how to improve the performance of the provisional 2007 PRES/ACR/EULAR pediatric criteria. The introduction of a multidimensional severity score, named "J4S," which includes parameters on growth, skin, and internal organ involvement, has improved the approach to the patients in the daily practice to guide decision-making...
August 2017: Best Practice & Research. Clinical Rheumatology
https://www.readbyqxmd.com/read/29768556/first-report-of-rhodnius-neglectus-hemiptera-reduviidae-triatominae-from-the-state-of-acre-brazil-and-the-brazilian-western-amazon-region
#12
Leandro José Ramos, Gabriela Vieira de Souza Castro, Janis Lunier de Souza, Jader de Oliveira, João Aristeu da Rosa, Luis Marcelo Aranha Camargo, Renildo Moura da Cunha, Dionatas Ulises de Oliveira Meneguetti
INTRODUCTION: This communication reports the occurrence of Rhodnius neglectus in the State of Acre, Brazil. METHODS: An adult male R. neglectus specimen was collected from the Catuaba Experimental Reserve, which is located in the municipality of Senador Guiomard. RESULTS: This increases the number of triatomine species reported from the State of Acre to eight and is also the first report of R. neglectus from the Brazilian Western Amazon Region...
March 2018: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29767833/post-operative-care-of-interventional-therapy-for-40-liver-cancer-patients-with-obstructive-jaundice
#13
De-Ping Tong, Li-Qin Wu, Xiao-Ping Chen, Yi Li
The care of 40 patients with primary liver cancer with obstructive jaundice treated with liver puncture bile drainage or biliary stent implantation was reported. Treated with the interventional therapy, patients were observed closely to identify symptoms of hepatic encephalopathy and pain; diet care was well performed. Bile drainage tube and skin acre were performed carefully. Liver function, bilirubin and other biochemical indicators were monitored; occurrence of bleeding, acute pancreatitis, biliary tract infection, leakage of ascites around drainage tube and other complication were observed with good discharge instruction...
May 16, 2018: European Journal of Cancer Care
https://www.readbyqxmd.com/read/29767043/study-of-bacillus-subtilis-on-growth-performance-nutrition-metabolism-and-intestinal-microflora-of-1-to-42-d-broiler-chickens
#14
Zhenhua Gao, Haohao Wu, Lin Shi, Xiaohui Zhang, Ran Sheng, Fuquan Yin, Ravi Gooneratne
To study the influence of different levels of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d Arbor Acres (AA) broilers, a total of 800 one-day-old healthy broilers were randomly divided into 5 groups with 4 replicates per group and 40 broilers per replicate. Broilers were fed a basic diet (group 1) which acted as the control group, and 4 other groups (2 to 5) were fed the basal diet with B. subtilis added at concentrations of 100, 150, 200 and 250 mg/kg, respectively for 42 days...
June 2017: Animal nutrition
https://www.readbyqxmd.com/read/29764746/appropriateness-and-clinical-outcome-of-chest-computed-tomography-without-intravenous-contrast-a-study-conducted-in-pakistan
#15
Amjad Sattar, Sohail Ahmed Khan, Nauman Al-Qamari, Hatem Adel, Syed Omair Adil, Kashif Shafique
BACKGROUND: Chest computed tomography (CT), including high-resolution CT (HRCT), has become an integral part of modern healthcare. It enables the physician to arrive at a diagnosis using a noninvasive approach. Our practice has shown that various chest CT scans without intravenous (IV) contrast, including HRCT, have no proper clinical indication. For the same reason, we have assessed the appropriateness of chest CT without IV contrast based on the evidence-based American College of Radiology (ACR) appropriateness criteria...
March 28, 2018: Respiratory Investigation
https://www.readbyqxmd.com/read/29764185/management-of-metastatic-spinal-cord-compression-among-veterans-health-administration-radiation-oncologists
#16
Ruchika Gutt, Sheeta Malhotra, Shruti Jolly, Drew Moghanaki, Alice V Cheuk, Helen Fosmire, Maria Kelly, Lori Hoffman-Hogg, Stephen Lutz, Mitchell Anscher, George Dawson
BACKGROUND: Optimal management of metastatic spinal cord compression (MSCC) improves functional outcomes in patients with metastatic disease. This survey study evaluated management of MSCC by Veterans Health Administration (VHA) radiation oncologists (ROs), to determine whether management of MSCC correlates with American College of Radiology (ACR) guidelines, and to compare times to initiation of treatment between surgery and radiotherapy (RT). METHODS: Surveys emailed to 79 VHA ROs included questions on steroid use, surgical care, palliative care, fractionation of irradiation, re-irradiation, and management of common MSCC case scenarios...
April 2018: Annals of Palliative Medicine
https://www.readbyqxmd.com/read/29761089/association-of-haemodynamic-indices-of-central-and-peripheral-pressure-with-subclinical-target-organ-damage
#17
Junli Zuo, Shaoli Chu, Isabella Tan, Mark Butlin, Jiehui Zhao, Alberto Avolio
Background: Central aortic pressure has often been shown to be more closely associated with markers of vascular function and incidence of cardiovascular events compared to peripheral pressure. However, the potential clinical use of central aortic or peripheral haemodynamic indices as markers of target organ damage (TOD) has not been fully established. Methods: We evaluated associations of TOD with central aortic and peripheral haemodynamic indices (central aortic [cPP] and peripheral pulse pressure [pPP], central aortic augmentation index, and central and peripheral waveform factor) in 770 hospital inpatients (age 60 ± 10 years, 473 males) with primary hypertension...
March 2018: Pulse (Basel, Switzerland)
https://www.readbyqxmd.com/read/29760842/appropriateness-of-extremity-magnetic-resonance-imaging-examinations-in-an-academic-emergency-department-observation-unit
#18
McKinley Glover, Ravi V Gottumukkala, Yadiel Sanchez, Brian J Yun, Theodore I Benzer, Benjamin A White, Anand M Prabhakar, Ali S Raja
Introduction: Emergency departments (ED) and hospitals face increasing challenges related to capacity, throughput, and stewardship of limited resources while maintaining high quality. Appropriate utilization of extremity magnetic resonance imaging (MRI) examinations within the emergency setting is not well known. Therefore, this study aimed to determine indications for and appropriateness of MRI of the extremities for musculoskeletal conditions in the ED observation unit (EDOU). Methods: We conducted this institutional review board-approved, retrospective study in a large, quaternary care academic center and Level I trauma center...
May 2018: Western Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29756770/versatile-high-throughput-fluorescence-assay-for-monitoring-cas9-activity
#19
Kyle Seamon, Yooli K Light, Edwin Saada, Joseph S Schoeniger, Brooke Harmon
The RNA-guided DNA nuclease Cas9 is now widely used for the targeted modification of genomes of human cells and various organisms. Despite the extensive use of CRISPR systems for genome engineering and the rapid discovery and engineering of new CRISPR-associated nucleases, there are no high-throughput assays for measuring enzymatic activity. The current laboratory and future therapeutic uses of CRISPR technology have a significant risk of accidental exposure or clinical off-target effects, under-scoring the need for therapeutically-effective inhibitors of Cas9...
May 14, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29755844/standard-osem-vs-regularized-pet-image-reconstruction-qualitative-and-quantitative-comparison-using-phantom-data-and-various-clinical-radiopharmaceuticals
#20
Judit Lantos, Erik S Mittra, Craig S Levin, Andrei Iagaru
We investigated the block sequential regularized expectation maximization (BSREM) algorithm. ACR phantom measurements with different count statistics and 60 PET/CT research scans from the GE Discovery 600 and 690 scanners were reconstructed using BSREM and the standard-of-care OSEM algorithm. Hot concentration recovery and cold contrast recovery were measured from the phantom data. Two experienced nuclear medicine physicians reviewed the clinical images blindly. Liver SNR liver and SUVmax of the smallest lesion detected in each patient were also measured...
2018: American Journal of Nuclear Medicine and Molecular Imaging
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