keyword
MENU ▼
Read by QxMD icon Read
search

BCAA

keyword
https://www.readbyqxmd.com/read/29324714/determination-of-branched-chain-keto-acids-in-serum-and-muscles-using-high-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#1
You Zhang, Bingjie Yin, Runxian Li, Pingli He
Branched-chain keto acids (BCKAs) are derivatives from the first step in the metabolism of branched-chain amino acids (BCAAs) and can provide important information on animal health and disease. Here, a simple, reliable and effective method was developed for the determination of three BCKAs (α-ketoisocaproate, α-keto-β-methylvalerate and α-ketoisovalerate) in serum and muscle samples using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS). The samples were extracted using methanol and separated on a 1...
January 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29315422/dietary-therapy-restores-glutamatergic-input-to-orexin-hypocretin-neurons-after-traumatic-brain-injury-in-mice
#2
Jonathan E Elliott, Samuel E De Luche, Madeline J Churchill, Cindy Moore, Akiva S Cohen, Charles K Meshul, Miranda M Lim
Study Objectives: In previous work, dietary branched chain amino acid (BCAA) supplementation, precursors to de novo glutamate and gamma-aminobutyric acid (GABA) synthesis, restored impaired sleep-wake regulation and orexin neuronal activity post-traumatic brain injury (TBI) in mice. TBI was speculated to reduce orexin neuronal activity through decreased regional excitatory (glutamate) and/or increased inhibitory (GABA) input. Therefore, we hypothesized TBI would decrease synaptic glutamate and/or increase synaptic GABA in nerve terminals contacting orexin neurons, and BCAA supplementation would restore TBI-induced changes in synaptic glutamate and/or GABA...
January 5, 2018: Sleep
https://www.readbyqxmd.com/read/29307017/clinical-characteristics-and-mutation-analysis-of-five-chinese-patients-with-maple-syrup-urine-disease
#3
Xiaomei Li, Yali Yang, Qing Gao, Min Gao, Yvqiang Lv, Rui Dong, Yi Liu, Kaihui Zhang, Zhongtao Gai
Maple syrup urine disease (MSUD) is an autosomal recessive disorder affecting branched-chain amino acids (BCAAs) metabolism and caused by a defect in the thiamine-dependent enzyme branched chain α-ketoacid dehydrogenase (BCKD) with subsequent accumulation of BCAAs and corresponding branched-chain keto acids (BCKAs) metabolites. Presently, at least 4 genes of BCKDHA, BCKDHB, DLD and DBT have been reported to cause MSUD. Furthermore, more than 265 mutations have been identified as the cause across different populations worldwide...
January 6, 2018: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29291007/alteration-of-muscle-fiber-characteristics-and-the-ampk-sirt1-pgc-1%C3%AE-axis-in-skeletal-muscle-of-growing-pigs-fed-low-protein-diets-with-varying-branched-chain-amino-acid-ratios
#4
Yehui Duan, Fengna Li, Wenlong Wang, Qiuping Guo, Chaoyue Wen, Yulong Yin
There mainly exists four major myosin heavy chains (MyHC) (i.e., I, IIa, IIx, and IIb) in growing pigs. The current study aimed to explore the effects of low-protein diets supplemented with varying branched-chain amino acids (BCAAs) on muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscles. Forty growing pigs (9.85 ± 0.35 kg) were allotted to 5 groups and fed with diets supplemented with varying leucine: isoleucine: valine ratios: 1:0.51:0.63 (20% crude protein, CP), 1:1:1 (17% CP), 1:0...
December 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/29288718/kr%C3%A3-ppel-like-factor-15-regulator-of-bcaa-metabolism-and-circadian-protein-rhythmicity
#5
REVIEW
Liyan Fan, Paishiun N Hsieh, David R Sweet, Mukesh K Jain
Regulation of nutrient intake, utilization, and storage exhibits a circadian rhythmicity that allows organisms to anticipate and adequately respond to changes in the environment across day/night cycles. The branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are important modulators of metabolism and metabolic health - for example, their catabolism yields carbon substrates for gluconeogenesis during periods of fasting. Krüppel-like factor 15 (KLF15) has recently emerged as a critical transcriptional regulator of BCAA metabolism, and the absence of this transcription factor contributes to severe pathologies such as Duchenne muscular dystrophy and heart failure...
December 27, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29281182/high-dietary-intakes-of-branched-chain-amino-acids-is-associated-with-increased-risk-of-insulin-resistance-in-adults
#6
Golaleh Asghari, Hossein Farhadnejad, Farshad Teymoori, Parvin Mirmiran, Maryam Tohidi, Fereidoun Azizi
BACKGROUND: The aim of this study was to investigate the association between branched-chain amino acids (BCAAs) intake and markers of insulin metabolism in adults. METHODS: This cohort study was conducted within the framework of the Tehran Lipid and Glucose Study (TLGS), on 1205 subjects, aged ≥ 20 years, who followed-up for a mean duration of 2.3 years. Dietary intakes of BCAAs including valine, leucine, and isoleucine were determined, using a valid and reliable food-frequency questionnaire...
December 27, 2017: Journal of Diabetes
https://www.readbyqxmd.com/read/29246479/elevated-plasma-levels-of-3-hydroxyisobutyric-acid-are-associated-with-incident-type-2-diabetes
#7
Adil Mardinoglu, Silvia Gogg, Luca A Lotta, Alena Stančáková, Annika Nerstedt, Jan Boren, Matthias Blüher, Ele Ferrannini, Claudia Langenberg, Nicholas J Wareham, Markku Laakso, Ulf Smith
Branched-chain amino acids (BCAAs) metabolite, 3-Hydroxyisobutyric acid (3-HIB) has been identified as a secreted mediator of endothelial cell fatty acid transport and insulin resistance (IR) using animal models. To identify if 3-HIB is a marker of human IR and future risk of developing Type 2 diabetes (T2D), we measured plasma levels of 3-HIB and associated metabolites in around 10,000 extensively phenotyped individuals. The levels of 3-HIB were increased in obesity but not robustly associated with degree of IR after adjusting for BMI...
December 7, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29244956/effects-of-carbohydrate-and-branched-chain-amino-acid-beverage-ingestion-during-acute-upper-body-resistance-exercise-on-performance-and-post-exercise-hormone-response
#8
JohnEric W Smith, Ben M Krings, Brandon D Shepherd, Hunter S Waldman, Steven A Basham, Matthew J McAllister
The purpose of this investigation was to examine the individual and combined effects of ingesting carbohydrates (CHO) and branched chain amino acids (BCAA) during high volume upper-body resistance exercise (RE) on markers of catabolism and performance. Thirteen resistance trained males completed four experimental trials with supplementation ingesting beverages containing CHO, BCAA, CHO+BCAA, or placebo (PLA) in a randomized, double-blind design. The beverages were ingested in 118 mL servings six times during a ~60 min RE session consisting of bench press, bent-over row, incline press, and close-grip row...
December 15, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/29241574/glucose-mediated-inactivation-of-amp-activated-protein-kinase-reduces-the-levels-of-l-type-amino-acid-transporter-1-mrna-in-c2c12-cells
#9
Yu Yamamoto, Ran Sawa, Ikumi Wake, Ayaka Morimoto, Yasuhiko Okimura
Branched chain amino acids (BCAAs) have protective effects against muscle atrophy. Although plasma BCAA concentrations are higher in patients with diabetes than in healthy subjects, diabetes is related to sarcopenia. We hypothesized that high glucose concentration reduces the quantity of BCAA transporters, and consequently, the effects of BCAAs are diminished despite their high levels. We examined whether glucose reduces the expression of L-type amino acid transporter 1 (LAT1), which transports neutral amino acids, including BCAA, in C2C12 myocytes...
November 2017: Nutrition Research
https://www.readbyqxmd.com/read/29240953/influence-of-excess-branched-chain-amino-acid-uptake-by-streptococcus-mutans-in-human-host-cells
#10
Takafumi Arimoto, Rei Yambe, Hirobumi Morisaki, Haruka Umezawa, Hideo Kataoka, Shohei Matsui, Hirotaka Kuwata
Oral streptococci, including cariogenic bacterium Streptococcus mutans, comprise a large percentage of human supragingival plaque, which contacts both tooth surfaces and gingiva. Eukaryotic cells are able to take up macromolecules and particles, including bacteria, by endocytosis. Increasing evidence indicates endocytosis may be used as an entry process by bacteria. We hypothesized some endocytosed bacteria might survive and obtain nutrients, such as amino acids, until they are killed. To verify this hypothesis, we focused on bacterial utilization of branched-chain amino acids (BCAAs; isoleucine, leucine, and valine) in host cells...
December 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29229387/mir-124-3p-affects-the-formation-of-intramuscular-fat-through-alterations-in-branched-chain-amino-acid-consumption-in-sheep
#11
Yangyang Pan, Jiongjie Jing, Liying Qiao, Jianhua Liu, Junxing Zhao, Lixia An, Baojun Li, Weiwei Wang, Chen Liang, Wenzhong Liu
Intramuscular fat is used to determine meat quality in animals; however, factors affecting branched chain amino acid (BCAA) catabolism, which fuels adipogenesis and lipogenesis, remain unclear. To better understand the post-transcriptional influence on BCAA catabolism during adipogenesis, we investigated the role of miR-124-3p. Stromal vascular fraction (SVF) cells were isolated from skeletal muscle of sheep, and induced to differentiate. We determined the roles of miR-124-3p and its predicted target, branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), in adipogenic differentiation and lipogenesis of SVFs after overexpressing or inhibiting miR-124-3p or BCKDHA, respectively...
December 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29221133/endogenous-glutamine-decrease-is-associated-with-pancreatic-cancer-progression
#12
Cecilia Roux, Chiara Riganti, Sammy Ferri Borgogno, Roberta Curto, Claudia Curcio, Valeria Catanzaro, Giuseppe Digilio, Sergio Padovan, Maria Paola Puccinelli, Monica Isabello, Silvio Aime, Paola Cappello, Francesco Novelli
Pancreatic ductal adenocarcinoma (PDAC) is becoming the second leading cause of cancer-related death in the Western world. The mortality is very high, which emphasizes the need to identify biomarkers for early detection. As glutamine metabolism alteration is a feature of PDAC, its in vivo evaluation may provide a useful tool for biomarker identification. Our aim was to identify a handy method to evaluate blood glutamine consumption in mouse models of PDAC. We quantified the in vitro glutamine uptake by Mass Spectrometry (MS) in tumor cell supernatants and showed that it was higher in PDAC compared to non-PDAC tumor and pancreatic control human cells...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29211698/branched-chain-amino-acid-metabolism-in-cancer
#13
Elitsa A Ananieva, Adam C Wilkinson
PURPOSE OF REVIEW: The current review aims to provide an update on the recent biomedical interest in oncogenic branched-chain amino acid (BCAA) metabolism, and discusses the advantages of using BCAAs and expression of BCAA-related enzymes in the treatment and diagnosis of cancers. RECENT FINDINGS: An accumulating body of evidence demonstrates that BCAAs are essential nutrients for cancer growth and are used by tumors in various biosynthetic pathways and as a source of energy...
January 2018: Current Opinion in Clinical Nutrition and Metabolic Care
https://www.readbyqxmd.com/read/29209542/skin-lesions-associated-with-nutritional-management-of-maple-syrup-urine-disease
#14
Jaraspong Uaariyapanichkul, Puthita Saengpanit, Ponghatai Damrongphol, Kanya Suphapeetiporn, Sirinuch Chomtho
Introduction: Maple syrup urine disease (MSUD) is an inborn error of branched chain amino acids (BCAAs) metabolism. We report an infant with MSUD who developed 2 episodes of cutaneous lesions as a result of isoleucine deficiency and zinc deficiency, respectively. Case Presentation: A 12-day-old male infant was presented with poor milk intake and lethargy. The diagnosis of MSUD was made based on clinical and biochemical data. Management and Outcome: Specific dietary restriction of BCAAs was given...
2017: Case Reports in Dermatological Medicine
https://www.readbyqxmd.com/read/29203860/adipose-tissue-mitochondrial-capacity-associates-with-long-term-weight-loss-success
#15
R Jokinen, R Rinnankoski-Tuikka, S Kaye, L Saarinen, S Heinonen, M Myöhänen, E Rappou, S Jukarainen, A Rissanen, A Pessia, V Velagapudi, K A Virtanen, E Pirinen, K H Pietiläinen
OBJECTIVES: We investigated whether (1) subcutaneous adipose tissue (SAT) mitochondrial capacity predicts weight loss success and (2) weight loss ameliorates obesity-related SAT mitochondrial abnormalities. METHODS: SAT biopsies were obtained from 19 clinically healthy obese subjects (BMI 34.6±2.7 kg/m2) during a weight-loss intervention (0, 5, and 12 months) and from 19 lean reference subjects (BMI 22.7±1.1 kg/m2) at baseline. Based on one-year weight loss outcome, the subjects were divided into two groups: continuous weight losers (WL, n=6) and weight regainers (WR, n=13)...
December 5, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29201035/effects-of-epsps-copy-number-variation-cnv-and-glyphosate-application-on-the-aromatic-and-branched-chain-amino-acid-synthesis-pathways-in-amaranthus-palmeri
#16
Manuel Fernández-Escalada, Ainhoa Zulet-González, Miriam Gil-Monreal, Ana Zabalza, Karl Ravet, Todd Gaines, Mercedes Royuela
A key enzyme of the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), is the known target of the widely used herbicide glyphosate. Glyphosate resistance in Amaranthus palmeri, one of the most troublesome weeds in agriculture, has evolved through increased EPSPS gene copy number. The aim of this work was to study the pleiotropic effects of (i) EPSPS increased transcript abundance due to gene copy number variation (CNV) and of (ii) glyphosate application on the aromatic amino acid (AAA) and branched chain amino acid (BCAA) synthesis pathways...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29198954/metabolic-reprogramming-of-hydrogenosomal-amino-acids-in-trichomonas-vaginalis-under-glucose-restriction
#17
Kuo-Yang Huang, Seow-Chin Ong, Chih-Ching Wu, Chia-Wei Hsu, Hsin-Chung Lin, Yi-Kai Fang, Wei-Hung Cheng, Po-Jung Huang, Cheng-Hsun Chiu, Petrus Tang
BACKGROUND: Glucose is the major energy source that is converted to pyruvate for ATP generation in the trichomonad hydrogenosome. Under glucose restriction (GR), the regulation of amino acids metabolism is crucial for trichomonad growth and survival. RNA-sequencing (RNA-seq) analysis has been used to identify differentially expressed genes in Trichomonas vaginalis under GR, leading to significant advances in understanding adaptive responses of amino acid metabolism to GR. However, the levels of amino acid metabolites modulated by GR are unknown in T...
November 21, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29197337/genome-wide-motif-predictions-of-bcarr-box-in-the-amino-acid-repressed-genes-of-lactobacillus-helveticus-cm4
#18
Naoyuki Yamamoto, Taketo Wakai
BACKGROUND: A BCARR (branched-chain amino acid responsive repressor) identified in proteolytic gene expressions in Lactobacillus helveticus is considered to negatively control transcriptions by binding to operator sites at the promoter regions in the presence of BCAAs. However, the distributions and regulatory potential of the BCARR in all genes repressed by BCAAs in CM4 remains unclear. RESULTS: A genome-wide search for the BCARR-box was conducted to clarify the contribution of BCARR in the regulation of amino acid metabolism in L...
December 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29196239/insulin-and-branched-chain-amino-acid-depletion-during-mouse-preimplantation-embryo-culture-programmes-body-weight-gain-and-raised-blood-pressure-during-early-postnatal-life
#19
Miguel A Velazquez, Bhavwanti Sheth, Stephanie J Smith, Judith J Eckert, Clive Osmond, Tom P Fleming
Mouse maternal low protein diet exclusively during preimplantation development (Emb-LPD) is sufficient to programme altered growth and cardiovascular dysfunction in offspring. Here, we use an in vitro model comprising preimplantation culture in medium depleted in insulin and branched-chain amino acids (BCAA), two proposed embryo programming inductive factors from Emb-LPD studies, to examine the consequences for blastocyst organisation and, after embryo transfer (ET), postnatal disease origin. Two-cell embryos were cultured to blastocyst stage in defined KSOM medium supplemented with four combinations of insulin and BCAA concentrations...
November 28, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29191093/branched-chain-amino-acids-regulate-type-i-tropocollagen-and-type-iii-tropocollagen-syntheses-via-modulation-of-mtor-in-the-skin
#20
Takumi Yamane, Yuka Morioka, Yasuyuki Kitaura, Ken Iwatsuki, Yoshiharu Shimomura, Yuichi Oishi
Branched-chain amino acids (BCAAs) exhibit many physiological functions. However, the potential link and mechanism between BCAA and skin function are unknown. We examined the effects of deletion of branched-chain α-keto acid dehydrogenase kinase (BDK), a key enzyme in BCAA catabolism, on type I and III tropocollagen syntheses in mice. Leucine and isoleucine levels were significantly lower in the skin of BDK-KO mice compared with wild-type mice. No changes in valine concentrations were observed. The levels of type I and III tropocollagen proteins and mRNAs (COL1A1 and COL3A1) were significantly lower in the skin of BDK-KO mice compared with wild-type mice...
December 1, 2017: Bioscience, Biotechnology, and Biochemistry
keyword
keyword
22092
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"