keyword
MENU ▼
Read by QxMD icon Read
search

DRG

keyword
https://www.readbyqxmd.com/read/29790813/-express-increased-nav1-7-expression-in-the-dorsal-root-ganglion-contributes-to-pain-hypersensitivity-after-plantar-incision-in-rats
#1
Jiaoli Sun, Ningbo Li, Guangyou Duan, Yi Liu, Shanna Guo, Cong Wang, Changmao Zhu, Xianwei Zhang
Postoperative pain remains a complex problem that is difficult to manage in the clinical context, seriously affecting rehabilitation and the quality of life of patients after surgery. Nociceptors, of which the cell bodies are located in the dorsal root ganglion (DRG), are crucial for initiating and conducting the pain signal. The peripheral voltage-gated sodium channels, including Nav1.7, which is mainly expressed in the DRG, are key to understanding the mechanism underlying postoperative pain. Nav1.7, in particular, of which mutations in the encoding gene (SCN9A) can determine whether pain occurs, has aroused most attention...
January 1, 2018: Molecular Pain
https://www.readbyqxmd.com/read/29790812/-express-nav1-7-is-phosphorylated-by-fyn-tyrosine-kinase-which-modulates-channel-expression-and-gating-in-a-cell-type-dependent-manner
#2
Yangyang Li, Tengteng Zhu, Huan Yang, Sulayman Dib-Hajj, Stephen Waxman, Ye Yu, Tian-Le Xu, Xiaoyang Cheng
Voltage-gated sodium channel Nav1.7 is a key molecule in nociception, and its dysfunction has been associated with various pain disorders. Here, we investigated the regulation of Nav1.7 biophysical properties by Fyn, a Src family tyrosine kinase. Nav1.7 was coexpressed with either constitutively active (FynCA) or dominant negative (FynDN) variants of Fyn kinase. FynCA elevated protein expression and tyrosine phosphorylation of Nav1.7 channels. Site-directed mutagenesis analysis identified two tyrosine residues (Y1470 and Y1471) located within the Nav1...
January 1, 2018: Molecular Pain
https://www.readbyqxmd.com/read/29789638/human-carbonic-anhydrase-8-aav8-gene-therapy-inhibits-nerve-growth-factor-signaling-producing-prolonged-analgesia-and-anti-hyperalgesia-in-mice
#3
Gerald Z Zhuang, Udita Upadhyay, Xiaoying Tong, Yuan Kang, Diana M Erasso, Eugene S Fu, Konstantinos D Sarantopoulos, Eden R Martin, Tim Wiltshire, Luda Diatchenko, Shad B Smith, William Maixner, Roy C Levitt
Carbonic anhydrase-8 (Car8; murine gene symbol) is an allosteric inhibitor of inositol trisphosphate receptor-1 (ITPR1), which regulates neuronal intracellular calcium release. We previously reported that wild-type Car8 overexpression corrects the baseline allodynia and hyperalgesia associated with calcium dysregulation in the waddle (wdl) mouse due to a 19 bp deletion in exon 8 of the Car8 gene. In this report, we provide preliminary evidence that overexpression of the human wild-type ortholog of Car8 (CA8 WT ), but not the reported CA8 S100P loss-of-function mutation (CA8 MT ), inhibits nerve growth factor (NGF)-induced phosphorylation of ITPR1, TrkA (NGF high-affinity receptor), and ITPR1-mediated cytoplasmic free calcium release in vitro...
April 24, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29786105/cannabinoid-win-55-212-2-mesylate-inhibits-tumor-necrosis-factor-%C3%AE-induced-expression-of-nitric-oxide-synthase-in-dorsal-root-ganglion-neurons
#4
Rong Tan, Lijun Cao
Tumor necrosis factor-α (TNF-α) is an established pain modulator in the peripheral nervous system. Elevated levels of TNF-α in dorsal root ganglion (DRG) neurons reportedly is critical for neuropathic pain processing. It has been shown that the production of nitric oxide, a key player in the development and maintenance of nociception, depends on the expression of nitric oxide synthases (NOSs) and their activities. Accumulating evidence also supports an important role of cannabinoids in modulating neuropathic pain...
May 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29785689/the-impact-of-hospital-costing-methods-on-cost-effectiveness-analysis-a-case-study
#5
José Leal, Stefania Manetti, James Buchanan
BACKGROUND: Several methods exist to cost hospital contacts when estimating the cost effectiveness of a new intervention. However, the implications of choosing a particular approach remain unclear. We compare the use of the three main diagnosis-related group (DRG)-based national unit costs in England to determine whether choice of approach can impact on economic evaluation results. METHODS: A cost-utility model was developed to compare secondary fracture prevention models of care for hip fracture patients, using data from large primary and hospital care administrative datasets in England...
May 22, 2018: PharmacoEconomics
https://www.readbyqxmd.com/read/29781158/role-of-brain-derived-neurotrophic-factor-in-the-pathogenesis-of-distention-associated-abdominal-pain-in-bowel-obstruction
#6
Y Fu, Y M Lin, J H Winston, R Radhakrishnan, L-Y M Huang, X Z Shi
BACKGROUND: Previous studies found that visceral sensitivity is increased in bowel obstruction (BO). We hypothesized that mechanical stress-induced expression of BDNF in smooth muscle cells (SMC) of the distended bowel plays a critical role in visceral hypersensitivity in BO by altering voltage-gated K+ channel (Kv ) activity in sensory neurons. METHODS: Partial colon obstruction was maintained in rats for 7 days. Colon-projecting neurons in the dorsal root ganglia (DRG, T13 to L2) were isolated for electrophysiological and gene expression studies...
May 21, 2018: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29772357/modelling-the-dorsal-root-ganglia-using-human-pluripotent-stem-cells-a-platform-to-study-peripheral-neuropathies
#7
Serena Viventi, Mirella Dottori
Sensory neurons of the dorsal root ganglia (DRG) are the primary responders to stimuli inducing feelings of touch, pain, temperature, vibration, pressure and muscle tension. They consist of multiple subpopulations based on their morphology, molecular and functional properties. Our understanding of DRG sensory neurons has been predominantly driven by rodent studies and using transformed cell lines, whereas less is known about human sensory DRG neurons simply because of limited availability of human tissue. Although these previous studies have been fundamental for our understanding of the sensory system, it is imperative to profile human DRG subpopulations as it is becoming evident that human sensory neurons do not share the identical molecular and functional properties found in other species...
May 14, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29771722/detection-of-deteriorating-patients-on-surgical-wards-outside-the-icu-by-an-automated-mews-based-early-warning-system-with-paging-functionality
#8
Axel R Heller, Sören T Mees, Benjamin Lauterwald, Christian Reeps, Thea Koch, Jürgen Weitz
BACKGROUND: The establishment of early warning systems in hospitals was strongly recommended in recent guidelines to detect deteriorating patients early and direct them to adequate care. Upon reaching predefined trigger criteria, Medical Emergency Teams (MET) should be notified and directed to these patients. The present study analyses the effect of introducing an automated multiparameter early warning score (MEWS)-based early warning system with paging functionality on 2 wards hosting patients recovering from highly complex surgical interventions...
May 16, 2018: Annals of Surgery
https://www.readbyqxmd.com/read/29769557/nde1-positively-regulates-oligodendrocyte-morphological-differentiation
#9
Shoko Shimizu, Yugo Ishino, Masaya Tohyama, Shingo Miyata
Oligodendrocytes, the myelin-forming cells in the central nervous system (CNS), undergo morphological differentiation characterized by elaborated branched processes to enwrap neuronal axons. However, the basic molecular mechanisms underlying oligodendrocyte morphogenesis remain unknown. Herein, we describe the essential roles of Nuclear Distribution E Homolog 1 (NDE1), a dynein cofactor, in oligodendrocyte morphological differentiation. In the mouse corpus callosum, Nde1 mRNA expression was detected in oligodendrocyte lineage cells at the postnatal stage...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29768200/sensory-afferents-use-different-coding-strategies-for-heat-and-cold
#10
Feng Wang, Erik Bélanger, Sylvain L Côté, Patrick Desrosiers, Steven A Prescott, Daniel C Côté, Yves De Koninck
Primary afferents transduce environmental stimuli into electrical activity that is transmitted centrally to be decoded into corresponding sensations. However, it remains unknown how afferent populations encode different somatosensory inputs. To address this, we performed two-photon Ca2+ imaging from thousands of dorsal root ganglion (DRG) neurons in anesthetized mice while applying mechanical and thermal stimuli to hind paws. We found that approximately half of all neurons are polymodal and that heat and cold are encoded very differently...
May 15, 2018: Cell Reports
https://www.readbyqxmd.com/read/29761119/antibodies-against-cell-adhesion-molecules-and-neural-structures-in-paraneoplastic-neuropathies
#11
Ana M Siles, Eugenia Martínez-Hernández, Josefa Araque, Jordi Diaz-Manera, Ricard Rojas-Garcia, Eduard Gallardo, Isabel Illa, Francesc Graus, Luis Querol
Objective: Paraneoplastic neurological syndromes (PNS) are rare neurological disorders in which ectopic expression of neural antigens by a tumor results in an autoimmune attack against the nervous system. Onconeural antibodies not only guide PNS diagnosis but may also help detecting underlying malignancies. Our project aims to uncover new potential antibodies in paraneoplastic neuropathies (PN). Methods: Thirty-four patients fulfilling diagnostic criteria of possible ( n = 9; 26...
May 2018: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/29758584/inhibition-of-sympathetic-sprouting-in-ccd-rats-by-lacosamide
#12
Yuying Wang, Fuquan Huo
BACKGROUND: Early hyperexcitability activity of injured nerve/neuron is critical for developing sympathetic nerve sprouting within dorsal root ganglia (DRG). Since lacosamide (LCM), an anticonvulsant, inhibits Na+ channel. The present study tried to test the potential effect of LCM on inhibiting sympathetic sprouting in vivo. METHODS: LCM (50 mg/kg) was daily injected intraperitoneally into rats subjected to chronic compression DRG (CCD), an animal model of neuropathic pain that exhibits sympathetic nerve sprouting, for the 1st 7 days after injury...
May 14, 2018: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/29758573/-assessment-of-coding-in-german-diagnosis-related-groups-system-in-otorhinolaryngology
#13
Maik Ellies, Berit Anders, Wolfgang Seger
BACKGROUND: Prospective analysis of assessment reports in otorhinolaryngology for the period 01-03-2011 to 31-03-2017 by the Health Advisory Boards in Lower Saxony and Bremen, Germany in relation to coding in the G-DRG-System. MATERIAL AND METHODS: The assessment reports were documented using a standardized database system developed on the basis of the electronic data exchange (DTA) by the Health Advisory Board in Lower Saxony. In addition, the documentation of the assessment reports according to the G-DRG system was used for assessment...
May 14, 2018: Das Gesundheitswesen
https://www.readbyqxmd.com/read/29756027/translatome-regulation-in-neuronal-injury-and-axon-regrowth
#14
Meir Rozenbaum, Marek Rajman, Ida Rishal, Indrek Koppel, Sandip Koley, Katalin F Medzihradszky, Juan A Oses-Prieto, Riki Kawaguchi, Paul S Amieux, Alma L Burlingame, Giovanni Coppola, Mike Fainzilber
Transcriptional events leading to outgrowth of neuronal axons have been intensively studied, but the role of translational regulation in this process is not well understood. Here, we use translatome analyses by ribosome pull-down and protein synthesis characterization by metabolic isotopic labeling to study nerve injury and axon outgrowth proteomes in rodent dorsal root ganglia (DRGs) and sensory neurons. We identify over 1600 gene products that are primarily translationally regulated in DRG neurons after nerve injury, many of which contain a 5'UTR cytosine-enriched regulator of translation (CERT) motif, implicating the translation initiation factor Eif4e in the injury response...
March 2018: ENeuro
https://www.readbyqxmd.com/read/29755449/immunization-elicits-antigen-specific-antibody-sequestration-in-dorsal-root-ganglia-sensory-neurons
#15
Manojkumar Gunasekaran, Prodyot K Chatterjee, Andrew Shih, Gavin H Imperato, Meghan Addorisio, Gopal Kumar, Annette Lee, John F Graf, Dan Meyer, Michael Marino, Christopher Puleo, Jeffrey Ashe, Maureen A Cox, Tak W Mak, Chad Bouton, Barbara Sherry, Betty Diamond, Ulf Andersson, Thomas R Coleman, Christine N Metz, Kevin J Tracey, Sangeeta S Chavan
The immune and nervous systems are two major organ systems responsible for host defense and memory. Both systems achieve memory and learning that can be retained, retrieved, and utilized for decades. Here, we report the surprising discovery that peripheral sensory neurons of the dorsal root ganglia (DRGs) of immunized mice contain antigen-specific antibodies. Using a combination of rigorous molecular genetic analyses, transgenic mice, and adoptive transfer experiments, we demonstrate that DRGs do not synthesize these antigen-specific antibodies, but rather sequester primarily IgG1 subtype antibodies...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29754969/pathways-to-drg-based-hospital-payment-systems-in-japan-korea-and-thailand
#16
Peter Leslie Annear, Soonman Kwon, Luca Lorenzoni, Stephen Duckett, Dale Huntington, John C Langenbrunner, Yuki Murakami, Changwoo Shon, Ke Xu
Countries in Asia are working towards achieving universal health coverage while ensuring improved quality of care. One element is controlling hospital costs through payment reforms. In this paper we review experiences in using Diagnosis Related Groups (DRG) based hospital payments in three Asian countries and ask if there is an "Asian way to DRGs". We focus first on technical issues and follow with a discussion of implementation challenges and policy questions. We reviewed the literature and worked as an expert team to investigate existing documentation from Japan, Republic of Korea, and Thailand...
May 7, 2018: Health Policy
https://www.readbyqxmd.com/read/29752567/inhibiting-ezh2-rescued-bupivacaine-induced-neuronal-apoptosis-in-spinal-cord-dorsal-root-ganglia-in-mice
#17
Jinwei Zheng, Junping Chen, Guorong Wu, Chaoshuang Wu, Ruichun Wang, Wei Wang
PURPOSE: In the present work, we intended to explore the function of enhancer of zeste homolog 2 (EZH2) in modulated anesthetic reagent bupivacaine-induced neuronal apoptosis in spinal cord dorsal root ganglia (DRG). METHODS: Murine DRG explant was treated with 5 mM bupivacaine in vitro to induce neuronal apoptosis, which was examined by a TUNEL assay. Protein and mRNA expressions of EZH2 in bupivacaine-treated DRG were examined by western blot and qRT-PCR assays...
May 11, 2018: Journal of Anesthesia
https://www.readbyqxmd.com/read/29751848/differentiation-of-adipose-derived-stem-cells-into-schwann-cell-like-cells-through-intermittent-induction-potential-advantage-of-cellular-transient-memory-function
#18
Xun Sun, Yun Zhu, He-Yong Yin, Zhi-Yuan Guo, Feng Xu, Bo Xiao, Wen-Li Jiang, Wei-Min Guo, Hao-Ye Meng, Shi-Bi Lu, Yu Wang, Jiang Peng
BACKGROUND: Peripheral nerve injury (PNI) is a worldwide issue associated with severe social and economic burden. Autologous nerve grafting, the gold standard treatment for peripheral nerve defects, still has a number of technical limitations. Tissue engineering technology is a novel therapeutic strategy, and mesenchymal stromal cells (MSCs) are promising seed cells for nerve tissue engineering. However, the efficiency of traditional methods for inducing the differentiation of MSCs to Schwann cell-like cells (SCLCs) remains unsatisfactory...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29750992/transcriptome-analysis-of-dorsal-root-ganglia-s-diabetic-neuropathy-reveals-mechanisms-involved-in-pain-and-regeneration
#19
Maria Carolina Pedro Athie, Andre Schwambach Vieira, Juliana Maia Teixeira, Gilson Gonçalves Dos Santos, Elayne Vieira Dias, Claudia Herrera Tambeli, Cesar Renato Sartori, Carlos A Parada
Peripheral diabetic neuropathy (DN) manifests in nearly 60% of diabetic patients, being pain its most debilitating symptom. Although electrophysiological and morphological aspects are well described, little is known about its development and progression, undermining effective therapies. Hyperglycemia and insulin signaling impairment are considered the triggering events of oxidative stress observed in the dying nerves, however there are still many gaps in the knowledge of intracellular plastic changes it generates...
May 8, 2018: Life Sciences
https://www.readbyqxmd.com/read/29749720/anti-calponin-3-autoantibodies-a-new-specificity-in-patients-with-sj%C3%A3-gren-s-syndrome
#20
Julius Birnbaum, Ahmet Hoke, Aliya Lalji, Peter Calabresi, Pavan Bhargava, Livia Casciola-Rosen
OBJECTIVE: Autoantibodies are clinically useful for phenotyping patients across the spectrum of autoimmune rheumatic diseases. Using serum from a patient with Sjögren's Syndrome (SS), we detected a new specificity by immunoblotting. The purpose of this study was to identify this autoantibody and to evaluate its disease specificity. METHODS: A prominent 40 kDa band was detected when immunoblots were performed using a SS patient serum and lysate from rat dorsal root ganglia (DRG)...
May 11, 2018: Arthritis & Rheumatology
keyword
keyword
22062
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"