keyword
MENU ▼
Read by QxMD icon Read
search

SARS VIRUS

keyword
https://www.readbyqxmd.com/read/29152966/the-knowledge-and-high-seroprevalence-of-hepatitis-a-in-a-high-risk-group-agricultural-reproductive-aged-women-in-the-southeastern-region-of-turkey
#1
Nebiye Yentür Doni, Zeynep Şimşek, Gülcan Gürses, Fadile Yıldız Zeyrek, Muhsin Akbaba
Background/aim: The aim of this study was to determine the seroprevalence of antihepatitis A virus (HAV), risk factors, and the knowledge of female farm workers living in the Southeastern Anatolia Region (SAR) of Turkey. Materials and methods: This representative cross-sectional survey was conducted between January and April of 2013 in the SAR. A total of 705 reproductive-aged women were randomly selected by clustering method using Epi Info software. The sera were analyzed for anti-HAV antibodies using ELISA...
August 23, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29152578/middle-east-respiratory-syndrome-coronavirus-nonstructural-protein-16-is-necessary-for-interferon-resistance-and-viral-pathogenesis
#2
Vineet D Menachery, Lisa E Gralinski, Hugh D Mitchell, Kenneth H Dinnon, Sarah R Leist, Boyd L Yount, Rachel L Graham, Eileen T McAnarney, Kelly G Stratton, Adam S Cockrell, Kari Debbink, Amy C Sims, Katrina M Waters, Ralph S Baric
Coronaviruses (CoVs) encode a mixture of highly conserved and novel genes, as well as genetic elements necessary for infection and pathogenesis, raising the possibility of common targets for attenuation and therapeutic design. In this study, we focused on highly conserved nonstructural protein 16 (NSP16), a viral 2'O-methyltransferase (2'O-MTase) that encodes critical functions in immune modulation and infection. Using reverse genetics, we disrupted a key motif in the conserved KDKE motif of Middle East respiratory syndrome CoV (MERS-CoV) NSP16 (D130A) and evaluated the effect on viral infection and pathogenesis...
November 2017: MSphere
https://www.readbyqxmd.com/read/29143192/middle-east-respiratory-syndrome-and-severe-acute-respiratory-syndrome-current-therapeutic-options-and-potential-targets-for-novel-therapies
#3
REVIEW
Julie Dyall, Robin Gross, Jason Kindrachuk, Reed F Johnson, Gene G Olinger, Lisa E Hensley, Matthew B Frieman, Peter B Jahrling
No specific antivirals are currently available for two emerging infectious diseases, Middle East respiratory syndrome (MERS) and severe acute respiratory syndrome (SARS). A literature search was performed covering pathogenesis, clinical features and therapeutics, clinically developed drugs for repurposing and novel drug targets. This review presents current knowledge on the epidemiology, pathogenesis and clinical features of the SARS and MERS coronaviruses. The rationale for and outcomes with treatments used for SARS and MERS is discussed...
November 15, 2017: Drugs
https://www.readbyqxmd.com/read/29114026/proofreading-deficient-coronaviruses-adapt-for-increased-fitness-over-long-term-passage-without-reversion-of-exoribonuclease-inactivating-mutations
#4
Kevin W Graepel, Xiaotao Lu, James Brett Case, Nicole R Sexton, Everett Clinton Smith, Mark R Denison
The coronavirus (CoV) RNA genome is the largest among the single-stranded positive-sense RNA viruses. CoVs encode a proofreading 3'-to-5' exoribonuclease within nonstructural protein 14 (nsp14-ExoN) that is responsible for CoV high-fidelity replication. Alanine substitution of ExoN catalytic residues [ExoN(-)] in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and murine hepatitis virus (MHV) disrupts ExoN activity, yielding viable mutant viruses with defective replication, up to 20-fold-decreased fidelity, and increased susceptibility to nucleoside analogues...
November 7, 2017: MBio
https://www.readbyqxmd.com/read/29112950/single-primer-isothermal-amplification-spia-combined-with-next-generation-sequencing-provides-complete-bovine-coronavirus-genome-coverage-and-higher-sequence-depth-compared-to-sequence-independent-single-primer-amplification-sispa
#5
Mette Myrmel, Veslemøy Oma, Mamata Khatri, Hanne H Hansen, Maria Stokstad, Mikael Berg, Anne-Lie Blomström
Coronaviruses are of major importance for both animal and human health. With the emergence of novel coronaviruses such as SARS and MERS, the need for fast genome characterisation is ever so important. Further, in order to understand the influence of quasispecies of these viruses in relation to biology, techniques for deep-sequence and full-length viral genome analysis are needed. In the present study, we compared the efficiency of two sequence-independent approaches [sequence-independent single primer amplification (SISPA) and single primer isothermal amplification (SPIA, represented by the Ovation kit)] coupled with high-throughput sequencing to generate the full-length genome of bovine coronavirus (BCoV) from a nasal swab...
2017: PloS One
https://www.readbyqxmd.com/read/29107426/synthesis-and-biological-evaluation-of-nh2-acyl-oseltamivir-analogues-as-potent-neuraminidase-inhibitors
#6
Kuanglei Wang, Fei Yang, Lihui Wang, Kemin Liu, Lu Sun, Bin Lin, Yaping Hu, Boyu Wang, Maosheng Cheng, Yongshou Tian
Neuraminidase inhibitors can deter nascent viruses from infecting intact cells by preventing their release from host cells. Herein, a neuraminidase inhibitor 11b absent of basic moieties was discovered in the process of searching for inhibitors targeting 150 cavity. It exhibited potent inhibitions against wild-type neuraminidases from group 1 (H5N1 and H1N1) and group 2 (H7N9) subtypes with IC50 values similar to those of oseltamivir carboxylate. Moreover, 11b showed moderate inhibitions against mutant neuraminidases from H5N1-H274Y and H1N1-H274Y with IC50 values of 2075 nM and 1382 nM, which were inferior to those of oseltamivir carboxylate (6095 nM and 4071 nM)...
December 1, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29064679/mutation-of-asn-475-in-the-venezuelan-equine-encephalitis-virus-nsp2-cysteine-protease-leads-to-a-self-inhibited-state
#7
Jaimee R Compton, Matthew J Mickey, Xin Hu, Juan J Marugan, Patricia M Legler
The alphaviral nsP2 cysteine protease of the Venezuelan equine encephalitis virus (VEEV) is a validated antiviral drug target. Clan CN proteases contain a cysteine protease domain that is intimately packed with an S-adenosyl-l-methionine-dependent RNA methyltransferase (SAM MTase) domain. Within a cleft formed at the interface of these two domains, the peptide substrate is thought to bind. The nucleophilic cysteine can be found within a conserved motif, (475)NVCWAK(480), which differs from that of papain ((22)CGSCWAFS(29))...
November 9, 2017: Biochemistry
https://www.readbyqxmd.com/read/29056462/the-sars-cov-fusion-peptide-forms-an-extended-bipartite-fusion-platform-that-perturbs-membrane-order-in-a-calcium-dependent-manner
#8
Alex L Lai, Jean K Millet, Susan Daniel, Jack H Freed, Gary R Whittaker
Coronaviruses are a major infectious disease threat, and include the pathogenic human pathogens of zoonotic origin: SARS-CoV and MERS-CoV. Entry of coronaviruses into host cells is mediated by the viral spike (S) protein, which is structurally categorized as a class I viral fusion protein, within the same group as influenza virus and HIV. However, S proteins have two distinct cleavage sites that can be activated by a much wider range of proteases. The exact location of the coronavirus fusion peptide (FP) has been disputed...
October 19, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29038024/structural-insights-of-a-self-assembling-9-residue-peptide-from-the-c-terminal-tail-of-the-sars-corona-virus-e-protein-in-dpc-and-sds-micelles-a-combined-high-and-low-resolution-spectroscopic-study
#9
Anirban Ghosh, Dipita Bhattacharyya, Anirban Bhunia
In recent years, several studies based on the interaction of self-assembling short peptides derived from Viroporins with model membranes, have improved our understanding of the molecular mechanism of Corona virus (CoV) infection under physiological conditions. In this study, we have characterized the mechanism of membrane interaction of a short, 9-residue peptide TK9 (T(55)VYVYSRVK(63)) that had been derived from the carboxyl terminal of the Severe Acute Respiratory Syndrome (SARS) Corona virus (SARS CoV) Envelope (E) protein...
October 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29031062/design-synthesis-and-biological-evaluations-of-n-hydroxy-thienopyrimidine-2-4-diones-as-inhibitors-of-hiv-reverse-transcriptase-associated-rnase-h
#10
Jayakanth Kankanala, Karen A Kirby, Andrew D Huber, Mary C Casey, Daniel J Wilson, Stefan G Sarafianos, Zhengqiang Wang
Human immunodeficiency virus (HIV) reverse transcriptase (RT) associated ribonuclease H (RNase H) is the only HIV enzymatic function not targeted by current antiviral drugs. Although various chemotypes have been reported to inhibit HIV RNase H, few have shown significant antiviral activities. We report herein the design, synthesis and biological evaluation of a novel N-hydroxy thienopyrimidine-2,3-dione chemotype (11) which potently and selectively inhibited RNase H with considerable potency against HIV-1 in cell culture...
December 1, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28988778/nmr-structure-and-localization-of-a-large-fragment-of-the-sars-cov-fusion-protein-implications-in-viral-cell-fusion
#11
Mukesh Mahajan, Deepak Chatterjee, Kannaian Bhuvaneswari, Shubhadra Pillay, Surajit Bhattacharjya
The lethal Coronaviruses (CoVs), Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) and most recently Middle East Respiratory Syndrome Coronavirus, (MERS-CoV) are serious human health hazard. A successful viral infection requires fusion between virus and host cells carried out by the surface spike glycoprotein or S protein of CoV. Current models propose that the S2 subunit of S protein assembled into a hexameric helical bundle exposing hydrophobic fusogenic peptides or fusion peptides (FPs) for membrane insertion...
October 5, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28945945/macromolecular-antiviral-agents-against-zika-ebola-sars-and-other-pathogenic-viruses
#12
Franziska Schandock, Camilla Frich Riber, Annika Röcker, Janis A Müller, Mirja Harms, Paulina Gajda, Kaja Zuwala, Anna H F Andersen, Kaja Borup Løvschall, Martin Tolstrup, Florian Kreppel, Jan Münch, Alexander N Zelikin
Viral pathogens continue to constitute a heavy burden on healthcare and socioeconomic systems. Efforts to create antiviral drugs repeatedly lag behind the advent of pathogens and growing understanding is that broad-spectrum antiviral agents will make strongest impact in future antiviral efforts. This work performs selection of synthetic polymers as novel broadly active agents and demonstrates activity of these polymers against Zika, Ebola, Lassa, Lyssa, Rabies, Marburg, Ebola, influenza, herpes simplex, and human immunodeficiency viruses...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28943043/discovery-of-potent-antiviral-hsv-1-quinazolinones-and-initial-structure-activity-relationship-studies
#13
Carla E Brown, Tiffany Kong, James McNulty, Leonardo D'Aiuto, Kelly Williamson, Lora McClain, Paolo Piazza, Vishwajit L Nimgaonkar
The discovery of antiviral activity of 2,3-disubstituted quinazolinones, prepared by a one-pot, three-component condensation of isatoic anhydride with amines and aldehydes, against Herpes Simplex Virus (HSV)-1 is reported. Sequential iterative synthesis/antiviral assessment allowed structure-activity relationship (SAR) generation revealing synergistic structural features required for potent anti-HSV-1 activity. The most potent derivatives show greater efficacy than acyclovir against acute HSV-1 infections in neurons and minimal toxicity to the host...
October 15, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28930535/leader-sequences-of-coronavirus-are-altered-during-infection
#14
Li Liu, Wei Chen
The life cycle of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) involves a unique process called discontinuous transcription by which a set of 3' coterminal subgenomic mRNAs (sgmRNA) with identical 5' leader sequences can be generated. The current study demonstrates that the replication intermediates of minus strand of subgenomic RNA (sgRNA) can be readily recovered from SARS-CoV infected cells. A novel sgmRNA (M-1) was identified as a short version of membrane (M) gene. Transcriptional regulatory sequences (TRS) of SARS-CoV and Mouse Hepatitis Virus (MHV) sgmRNAs contain a species specific core element (CE)...
January 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/28923387/characterization-and-structure-activity-relationship-study-of-iminodipyridinopyrimidines-as-novel-hepatitis-c-virus-inhibitor
#15
Dong-Sik Park, Eunji Jo, Jihyun Choi, MyungEun Lee, Soohyun Kim, Hee-Young Kim, Jiyon Nam, Sujin Ahn, Jong Yeon Hwang, Marc Peter Windisch
Upon high-throughput screening of synthetic small molecule libraries with the infectious hepatitis C virus (HCV) cell culture system, we identified an iminodipyridinopyrimidine (IDPP) scaffold. IDPP did not inhibit HCV replication, but exhibited very potent inhibitory activity on early and late steps of HCV life cycle. Applying an intensive structure-activity relationship (SAR) study, a promising IDPP Lead compound (12c) with excellent potency (EC50 = 10 nM), high safety margin (SI > 2000), and an acceptable stability in human and rat liver microsomes (t1/2 >60 min) was identified...
November 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28923111/a-novel-coltivirus-related-virus-isolated-from-free-tailed-bats-from-c%C3%A3-te-d-ivoire-is-able-to-infect-human-cells-in-vitro
#16
Sabrina Weiss, Piotr Wojtek Dabrowski, Andreas Kurth, Siv Aina J Leendertz, Fabian H Leendertz
BACKGROUND: Zoonotic transmission events play a major role in the emergence of novel diseases. While such events are virtually impossible to predict, wildlife screening for potential emerging pathogens can be a first step. Driven by recent disease epidemics like severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Ebola, bats have gained special interest as reservoirs of emerging viruses. METHODS: As part of a bigger study investigating pathogens in African bats we screened animals for the presence of known and unknown viruses...
September 18, 2017: Virology Journal
https://www.readbyqxmd.com/read/28885377/clinical-pearls-in-venovenous-extracorporeal-life-support-for-adult-respiratory-failure
#17
Chee Kiang Tay, Kiick Sung, Yang Hyun Cho
Extracorporeal life support (ECLS) has been widely utilized to treat neonatal respiratory failure for two decades. However, its uptake in the case of adult respiratory failure has been slow because of a paucity of quality evidence and a sluggish tempo of ECLS-related technological advances. In recent years, interest in ECLS has been piqued after encouraging results were reported from its use during the 2009 H1N1 influenza pandemic. In a world constantly under threat from another influenza epidemic or deadly novel respiratory infection, e...
September 6, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28883814/virucidal-influence-of-ionic-liquids-on-phages-p100-and-ms2
#18
Susanne Fister, Patrick Mester, Julia Sommer, Anna K Witte, Roland Kalb, Martin Wagner, Peter Rossmanith
An increasing number of publications describe the potential of ionic liquids (ILs) as novel antimicrobials, antibacterial coatings and even as active pharmaceutical ingredients. Nevertheless, a major research area, notably their impact on viruses, has so far been neglected. Consequently the aim of this study was to examine the effects of ILs on the infectivity of viruses. A systematic analysis to investigate the effects of defined structural elements of ILs on virus activity was performed using 55 ILs. All structure activity relationships (SARs) were tested on the human norovirus surrogate phage MS2 and phage P100 representing non-enveloped DNA viruses...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28870104/type-ii-transmembrane-serine-proteases-as-potential-target-for-anti-influenza-drug-discovery
#19
REVIEW
Woo-Jin Shin, Baik Lin Seong
The outbreak of an influenza pandemic as well as the continued circulation of seasonal influenza highlights the need for effective antiviral therapies. The emergence of drug-resistant strains further necessitates the development of novel antivirals that target the host factors crucial for viral replication. Area covered: This review summarizes the current understanding of the structural and functional properties of type II transmembrane serine proteases (TTSPs) as a proteolytic activator of influenza virus infection and discusses their potential as antiviral targets...
November 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28866271/novel-%C3%AE-%C3%AE-unsaturated-amide-derivatives-bearing-%C3%AE-amino-phosphonate-moiety-as-potential-antiviral-agents
#20
Xianmin Lan, Dandan Xie, Limin Yin, Zhenzhen Wang, Jin Chen, Awei Zhang, Baoan Song, Deyu Hu
Based on flexible construction and broad bioactivity of ferulic acid, a series of novel α,β-unsaturated amide derivatives bearing α-aminophosphonate moiety were designed, synthesized and systematically evaluated for their antiviral activity. Bioassay results indicated that some compounds exhibited good antiviral activities against cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV) in vivo. Especially, compound g18 showed excellent curative and protective activities against CMV, with half-maximal effective concentration (EC50) values of 284...
September 15, 2017: Bioorganic & Medicinal Chemistry Letters
keyword
keyword
22048
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"