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https://www.readbyqxmd.com/read/28922632/antibacterial-and-anti-biofouling-coating-on-hydroxyapatite-surface-based-on-peptide-modified-tannic-acid
#1
Xiao Yang, Pei Huang, Haihuan Wang, Shuang Cai, Yixue Liao, Ziqin Mo, Xinyuan Xu, Chunmei Ding, Changsheng Zhao, Jianshu Li
Hydroxyapatite-based implant is widely used in the fields of artificial teeth and bones. However, the problem of microbial communities on hydroxyapatite surfaces remain a great challenge. In order to inhibit bacterial adhesion and activity in a long term, a salivary acquired pellicle (SAP) bioinspired tannic acid (SAP3-TA) is synthesized and characterized. The SAP inspired peptide sequence DDDEEK has a good adsorption performance on the surface of hydroxyapatite (HA). Tannic acid (TA) is a natural polyphenolic compound that can be extracted from green tea, and it has been proved to have an effective antibacterial property...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28922613/the-etiology-of-community-acquired-pneumonia-among-children-under-5%C3%A2-years-of-age-in-mainland-china-2001-2015-a-systematic-review
#2
Guijun Ning, Xuxia Wang, Dan Wu, Zundong Yin, Yixing Li, Huaqing Wang, Weizhong Yang
BACKGROUND: The aim of this systematic review was to examine the etiology of community-acquired pneumonia (CAP) among Chinese children younger than 5 years and provide evidence for further cost-effectiveness analyses for vaccine development, diagnostic strategies and empirical treatments. METHODS: The literature review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement. Data were obtained by searching PubMed, Embase, Web-of Science, and the Chinese databases Wanfang Data and China National Knowledge Infrastructure...
September 18, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28922566/streptococcus-pneumoniae-and-chronic-endobronchial-infections-in-childhood
#3
Kim M Hare, Amanda J Leach, Heidi C Smith-Vaughan, Anne B Chang, Keith Grimwood
Streptococcus pneumoniae (pneumococcus) is the main cause of bacterial pneumonia worldwide and has been studied extensively in this context. However, its role in chronic endobronchial infections and accompanying lower airway neutrophilic infiltration has received little attention. Severe and recurrent pneumonia are risk factors for chronic suppurative lung disease (CSLD) and bronchiectasis; the latter causes considerable morbidity and, in some populations, premature death in children and adults. Protracted bacterial bronchitis (PBB) is another chronic endobronchial infection associated with substantial morbidity...
September 18, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28922066/the-effect-of-lysozyme-on-reducing-biofilms-by-staphylococcus-aureus-pseudomonas-aeruginosa-and-gardnerella-vaginalis-an-in-vitro-examination
#4
Mirsada Hukić, Dzenita Seljmo, Amra Ramovic, Monia Avdić Ibrišimović, Serkan Dogan, Jasna Hukic, Elma Feric Bojic
Two basic questions about lysozyme activities on the selected microorganisms were investigated, namely whether lysozyme inhibits biofilm production and which concentrations of the enzyme have the ability to change the natural biofilm producing capacity of different strains of Staphylococcus aureus (methicillin sensitive and resistant), Streptococcus pyogenes, Pseudomonas aeruginosa, and Gardnerella vaginalis. The effect of lysozyme on biofilm formation capacities of 16 strains of selected microorganisms was investigated, whereby four testing replicates have been performed in vitro using the Test Tube method, and the potential of lysozyme to change biofilm forming capacities depending on its concentration, species, and strains of microorganisms is demonstrated...
September 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28921941/the-lactococcus-lactis-kf147-nonribosomal-peptide-synthetase-polyketide-synthase-system-confers-resistance-to-oxidative-stress-during-growth-on-plant-leaf-tissue-lysate
#5
Benjamin L Golomb, Annabelle O Yu, Laurynne C Coates, Maria L Marco
Strains of Lactococcus lactis isolated from plant tissues possess adaptations that support their survival and growth in plant-associated microbial habitats. We previously demonstrated that genes coding for a hybrid nonribosomal peptide synthetase/polyketide synthase (NRPS/PKS) system involved in production of an uncharacterized secondary metabolite are specifically induced in L. lactis KF147 during growth on plant tissues. Notably, this NRPS/PKS has only been identified in plant-isolated strains of L. lactis...
September 18, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28921547/a-clinical-randomized-study-on-the-influence-of-dental-whitening-on-streptococcus-mutans-population
#6
André Luiz Fraga Briso, Úrsula Aparecida Escalero Silva, Morganna Borges de Almeida Souza, Vanessa Rahal, Elerson Gaetti Jardim Júnior, Luciano Tavares Angelo Cintra
Dental whitening with peroxides has been popularized through the at-home technique, which employs low concentrations of peroxide applied on individual trays. However, there are few clinical trials reporting the effects of its continuous use on oral microbiota. Thus, the purpose of the present clinical, randomized study was to evaluate the influence of at-home whitening treatment on Streptococcus mutans in saliva, buccal mucosa, and subgingival and supragingival plaque. Thirty volunteers were randomly divided into 2 study groups (n=15) according to the whitening therapy: G CP- whitening using 10% carbamide peroxide 4 hours daily for 21 days and G HP- whitening using 6% hydrogen peroxide 1...
September 16, 2017: Australian Dental Journal
https://www.readbyqxmd.com/read/28920845/modified-use-of-real-time-pcr-detection-of-group-b-streptococcus-in-pregnancy
#7
Ali A Rabaan, Justin V Saunar, Ali M Bazzi, Joan L Soriano
The CDC recommends antenatal screening of vaginal/rectal samples for Streptococcus agalactiae at 35-37 weeks' gestation, with intra-partum antibiotic prophylaxis for positive cases. We tested a modified use of the Cepheid Xpert GBS real-time PCR kit on enrichment cultures from 554 vaginal/rectal swabs compared to the current subculturing gold standard method. Swabs were inoculated on polymyxin nalidixic acid agar plates, and Todd-Hewitt enrichment broth cultures were examined daily for growth. Todd-Hewitt broth culture was also used for Xpert GBS...
September 18, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28920437/real-time-metabolic-interactions-between-two-bacterial-species-using-a-carbon-based-ph-microsensor-as-a-scanning-electrochemical-microscopy-secm-probe
#8
Vrushali Subhash Joshi, Partha Sarathi Sheet, Nyssa Cullin, Jens Kreth, Dipankar Koley
We have developed a carbon-based, fast-response potentiometric pH microsensor for use as a scanning electrochemical microscopy (SECM) chemical probe to quantitatively map the microbial metabolic exchange between two bacterial species, commensal Streptococcus gordonii and pathogenic Streptococcus mutans. The 25-μm diameter H+ ion-selective microelectrode or pH microprobe showed a Nernstian slope of 59 mV/pH and high selectivity against major ions such Na+, K+, Ca2+ and Mg2+. In addition, the unique conductive membrane composition aided us in performing an amperometric approach curve to position the probe and obtain a high-resolution pH map of the microenvironment produced by the lactate-producing S...
September 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28919694/incidence-of-bovine-clinical-mastitis-in-jammu-region-and-antibiogram-of-isolated-pathogens
#9
Adil Majid Bhat, Jasvinder Singh Soodan, Rajiv Singh, Ishfaq Ahmad Dhobi, Tufail Hussain, Mohammad Yousuf Dar, Muheet Mir
AIM: This study was conducted to evaluate the incidence of clinical mastitis in bovines of Jammu region, to identify the infectious organisms responsible for it, and the antimicrobial sensitivity of isolated pathogens. MATERIALS AND METHODS: The study was conducted on cases that were presented to the Medicine Division of Teaching Veterinary Clinical Complex, Faculty of Veterinary Sciences and Animal Husbandry, R.S. Pura, Jammu, Jammu and Kashmir. A total of 260 cases of bovines were presented from June 30, 2012, to July 01, 2013, out of which 30 cases were of clinical mastitis...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28919415/biophysical-characterization-of-the-interaction-between-heme-and-proteins-responsible-for-heme-transfer-in-streptococcus-pyogenes
#10
Masato Hoshino, Makoto Nakakido, Satoru Nagatoishi, Chihiro Aikawa, Ichiro Nakagawa, Kouhei Tsumoto
Streptococcus pyogenes, an important pathogen that causes a wide range of diseases, possesses the sia gene cluster, which encodes proteins involved in the heme acquisition system. Although this system was previously described, the molecular mechanism of effective heme transfer remains to be elucidated. Here, we have characterized the interactions between heme and each domain of Streptococcal hemoprotein receptor (Shr) and Streptococcal heme-binding protein (Shp). Our kinetic and thermodynamic analyses suggested that effective heme transfer within this system is achieved not only by affinity-based transfer but also by the difference of the binding driving force...
September 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28919333/status-of-the-effectiveness-of-contact-lens-disinfectants-in-malaysia-against-keratitis-causing-pathogens
#11
Farhat Abjani, Naveed Ahmed Khan, Suk Yul Jung, Ruqaiyyah Siddiqui
The aim of this study was (i) to assess the antimicrobial effects of contact lens disinfecting solutions marketed in Malaysia against common bacterial eye pathogens and as well as eye parasite, Acanthamoeba castellanii, and (ii) to determine whether targeting cyst wall would improve the efficacy of contact lens disinfectants. Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal and amoebicidal assays of six different contact lens solutions including Oxysept(®), AO SEPT PLUS, OPTI-FREE(®) pure moist(®), Renu(®) fresh™, FreshKon(®) CLEAR and COMPLETE RevitaLens™ were performed using Manufacturers Minimum recommended disinfection time (MRDT)...
September 14, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28918832/streptococcus-anginosus-group-bacterial-infections
#12
Tasaduq Fazili, Scott Riddell, Deanna Kiska, Tim Endy, Luca Giurgea, Calden Sharngoe, Waleed Javaid
BACKGROUND: The Streptococcus anginosus group (SAG) causes a variety of infections in adults. To better understand the burden of SAG infections and their associated morbidity and mortality, we conducted a retrospective analysis of these infections in adults at a tertiary care center. METHODS: A retrospective review of all cultures positive for SAG in adults and a corresponding review of the patients' medical records were conducted at a tertiary care facility in central New York...
September 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28918242/predictive-value-of-thomsen-friedenreich-antigen-activation-for-streptococcus-pneumoniae-infection-and-severity-in-pediatric-lobar-pneumonia
#13
Chia-Jung Chang, Nan-Chang Chiu, Fu-Yuan Huang, Daniel Tsung-Ning Huang, Lung Chang, Ching-Ying Huang, Yen-Hsin Kung, Hsin Chi
BACKGROUND: Most cases of complicated pneumonia in children are caused by pneumococcal infections. Thomsen-Friedenreich antigen (TA) is present on erythrocytes, platelets and glomeruli, and it can be activated during pneumococcal infection. The aim of this study was to investigate the predictive value of TA activation for pneumococcal infection and association with the severity of complicated pneumonia. MATERIALS AND METHODS: Patients with lobar pneumonia were routinely tested for TA at the Department of Pediatrics, Mackay Memorial Hospital from January 2010 to December 2015...
September 2, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28918144/short-communication-molecular-epidemiology-of-streptococcus-agalactiae-differs-between-countries
#14
J Reyes, M Chaffer, Juan Carlos Rodriguez-Lecompte, Javier Sánchez, Ruth N Zadoks, Natasha Robinson, Ximena Cardona, N Ramírez, G P Keefe
Group B Streptococcus or Streptococcus agalactiae continue to be challenging for milk quality programs in countries with emerging dairy industries, such as Colombia, where high prevalence has been reported. Molecular typing of isolates is needed to understand the variability and epidemiology of this pathogen and to develop effective control and eradication programs. We characterized the molecular profile of Strep. agalactiae isolated from cows with subclinical mastitis in 21 Colombian dairy herds and measured diversity within and between herds using multilocus sequence typing...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917068/group-a-streptococcal-bacteraemia-experience-at-king-fahad-medical-city-in-riyadh-saudi-arabia
#15
Fawzyh J Al-Khadidi, Mohammed A AlSheheri, Tariq S AlFawaz, Mushira A Enani, Abdulaziz A AlAqeel, Dayel A AlShahrani
To identify clinical presentation, predisposing factors, and the outcome in patients with Group A Streptococcal bacteremia. Methods: This is a retrospective study of 33 pediatric and adult patients with Streptococcus pyogenes bacteremia, admitted at King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia from 2007 to 2015. Results: Thirty-three patient records, documenting bacteremia with group A beta-hemolytic streptococcus, were reviewed. Approximately 51.5% were pediatric and 48.5% were adult patients, with a male to female ratio of 2:1...
October 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28916465/corrosive-effects-of-fluoride-on-titanium-under-artificial-biofilm
#16
Azusa Fukushima, Gen Mayanagi, Keiichi Sasaki, Nobuhiro Takahashi
PURPOSE: This study aimed to investigate the effect of sodium fluoride (NaF) on titanium corrosion using a biofilm model, taking environmental pH into account. METHODS: Streptococcus mutans cells were used as the artificial biofilm, and pH at the bacteria-titanium interface was monitored after the addition of 1% glucose with NaF (0, 225 or 900ppmF) at 37°C for 90min. In an immersion test, the titanium samples were immersed in the NaF solution (0, 225 or 900ppm F; pH 4...
September 12, 2017: Journal of Prosthodontic Research
https://www.readbyqxmd.com/read/28916389/removal-and-killing-of-multispecies-endodontic-biofilms-by-n-acetylcysteine
#17
Young-Suk Choi, Cheul Kim, Ji-Hoi Moon, Jin-Yong Lee
Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans and Enterococcus faecalis on sterile human dentin blocks. The biofilms were exposed to NAC (25, 50 and 100mg/mL), saturated calcium hydroxide or 2% chlorhexidine solution for 7 days, then examined by scanning electron microscopy...
September 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28916372/tracking-and-controlling-soft-surface-contamination-in-health-care-settings
#18
Jonathan D Sexton, Amanda M Wilson, Hannah P Sassi, Kelly A Reynolds
BACKGROUND: Study objectives were to track the transfer of microbes on soft surfaces in health care environments and determine the efficiency of an Environmental Protection Agency (EPA)-registered soft surface sanitizer in the health care environment. METHODS: Soft surfaces at 3 health care facilities were sampled for heterotrophic plate count (HPC) bacteria, Staphylococcus spp, Streptococcus pyogenes, and Escherichia coli followed by a tracer study with a virus surrogate seeded onto volunteer hands and commonly touched surfaces...
September 12, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28916324/in%C3%A2-vitro-investigation-of-the-antimicrobial-effect-of-three-bisphosphonates-against-different-bacterial-strains
#19
Michael A Ermer, Simon C Kottmann, Jörg-Elard Otten, Annette Wittmer, Philipp Poxleitner, Klaus Pelz
PURPOSE: Since the first descriptions of medication-related osteonecrosis of the jaw (MRONJ) in 2003, the pathogenesis has remained unanswered. Recent histomorphometric studies have found several microorganisms, including Actinomyces, Bacillus, Fusobacterium, Staphylococcus, Streptococcus, Selenomonas, Treponema, and Candida albicans in necrotic bone. Polymerase chain reaction studies have recently confirmed the occurrence of 48 genera. Only a few studies have examined the antimicrobial effect of bisphosphonates (BPs)...
August 31, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28916315/the-effect-of-immune-modulation-of-streptococcus-constellatus-sc10-strain-upon-acinetobactor-baumannii-infection
#20
Abdullah A Alyousef, Israa M S Al-Kadmy
Acinetobacter baumannii is a tiny, plump and rod-shaped Gram-negative coccobacillus, notorious as an opportunistic pathogen, which is now being considered by CDC as a serious public health threat. Preventing colonization may be a better therapeutic choice using microbiota to protect against A. baumannii transmission. Human microbiota not only regulates immune homeostasis as well provides essential health benefits therefore discovering the interface due microbiota in host immune system will allow to recognise novel immuno- or microbe-based therapies...
September 12, 2017: Microbial Pathogenesis
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