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https://www.readbyqxmd.com/read/28449183/noninferiority-trial-investigating-the-efficacy-of-a-nonantibiotic-intramammary-therapy-in-the-treatment-of-mild-to-moderate-clinical-mastitis-in-dairy-cows-with-longer-lasting-udder-diseases
#1
M Ziesch, N Wente, Y Zhang, W Zaremba, S Engl, V Krömker
A nonblinded, positively controlled, noninferiority trial was conducted to evaluate the efficacy of an alternative, nonantibiotic therapy with Masti Veyxym(®) to reduce ineffective antibiotic usage in the treatment of nonsevere clinical mastitis (CM) in cows with longer lasting udder diseases. The solely intramammary treatment with Masti Veyxym(®) (three applications, 12 hr apart) and the combined treatment with Masti Veyxym(®) and antibiotics as usual on the farm according to label of the respective product were compared with the reference treatment of solely antibiotic therapy...
April 27, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28448807/alternative-sweeteners-influence-the-biomass-of-oral-biofilm
#2
Fathilah Abdul Razak, Baizatul Amirah Baharuddin, Elisya Farha Mohd Akbar, Amira Hanim Norizan, Nur Fazilah Ibrahim, Md Yusoff Musa
OBJECTIVE: Compact-structured oral biofilm accumulates acids that upon prolonged exposure to tooth surface, causes demineralisation of enamel. This study aimed to assess the effect of alternative sweeteners Equal Stevia(®), Tropicana Slim(®), Pal Sweet(®) and xylitol on the matrix-forming activity of plaque biofilm at both the early and established stages of formation. METHODS: Saliva-coated glass beads (sGB) were used as substratum for the adhesion of a mixed-bacterial suspension of Streptococcus mutans, Streptococcus sanguinis and Streptococcus mitis...
April 20, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28448633/a-commensal-streptococcus-hijacks-a-pseudomonas-aeruginosa-exopolysaccharide-to-promote-biofilm-formation
#3
Jessica A Scoffield, Dingyu Duan, Fan Zhu, Hui Wu
Pseudomonas aeruginosa causes devastating chronic pulmonary infections in cystic fibrosis (CF) patients. Although the CF airway is inhabited by diverse species of microorganisms interlaced within a biofilm, many studies focus on the sole contribution of P. aeruginosa pathogenesis in CF morbidity. More recently, oral commensal streptococci have been identified as cohabitants of the CF lung, but few studies have explored the role these bacteria play within the CF biofilm. We examined the interaction between P...
April 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28448066/structural-basis-of-crispr-spycas9-inhibition-by-an-anti-crispr-protein
#4
De Dong, Minghui Guo, Sihan Wang, Yuwei Zhu, Shuo Wang, Zhi Xiong, Jianzheng Yang, Zengliang Xu, Zhiwei Huang
The CRISPR-Cas9 systems are bacterial encoded adaptive immune systems to defend against phages infection, through RNA-guided endonuclease activity of Cas9 to degrade double-stranded DNA bearing a protospacer adjacent motif (PAM) and complementary sequences to the guide RNA1,2,3,4,5. Recently, two anti-CRISPR proteins AcrIIA2 and AcrIIA4 from Listeria monocytogenes (Lmo) prophages have been identified, both of which potently inhibit Streptococcus pyogenes Cas9 (SpyCas9) and LmoCas9 activity in bacteria and human cells6...
April 27, 2017: Nature
https://www.readbyqxmd.com/read/28447330/seawater-drowning-associated-pneumonia-a-10-year-descriptive-cohort-in-intensive-care-unit
#5
Alexandre Robert, Pierre-Éric Danin, Hervé Quintard, Nicolas Degand, Nihal Martis, Denis Doyen, Céline Pulcini, Raymond Ruimy, Carole Ichai, Gilles Bernardin, Jean Dellamonica
BACKGROUND: Pneumonia is one of the major complications of drowning, but the optimal empirical antibiotic treatment is not clearly defined. Multidrug-resistant (MDR) bacteria and fungi have been identified in a recent series of freshwater drowning-associated pneumonia. However, microbial data in seawater drowning are scarce. The objective of the study is to describe the microorganisms isolated in early respiratory specimens obtained from seawater drowning-associated pneumonia and to provide their antibiotic susceptibility pattern...
December 2017: Annals of Intensive Care
https://www.readbyqxmd.com/read/28447173/clinical-and-microbiological-features-associated-with-group-b-streptococcus-bone-and-joint-infections-france-2004-2014
#6
S Kernéis, C Plainvert, J-P Barnier, A Tazi, N Dmytruk, B Gislain, J Loubinoux, F El Sayed, V Cattoir, N Desplaces, V Vernet, P Morand, C Poyart
This study describes the clinical and microbiological features associated with group B Streptococcus (GBS) bone and joint infections (BJIs). It was a retrospective analysis of adult cases of GBS BJIs reported to the French National Reference Center for Streptococci from January 2004 to December 2014. Clinical data and GBS molecular characteristics are reported. Strains were collected from 163 patients. The most frequent comorbidities were: solid organ cancer (n = 21, 21%) and diabetes mellitus (n = 20, 20%)...
April 26, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28446575/evaluation-of-strepb-select-chromagar-and-the-fast-track-diagnostics-group-b-streptococcus-gbs-real-time-polymerase-chain-reaction-assay-compared-to-routine-culture-for-detection-of-gbs-during-antepartum-screening
#7
Deirdre L Church, Heather Baxter, Tracie Lloyd, Oscar Larios, Daniel B Gregson
Life-threatening infection in neonates due to Group B Streptococcus (GBS) is preventable by screening near-term pregnant women and treatment at delivery. 295 vaginal-rectal swabs were collected from women attending antepartum clinics in Calgary. GBS colonization was detected by the standard culture method (Strep B Carrot broth subbed to blood agar with a neomycin disk), and compared to recovery using Strep Group B Broth (Dalynn Biologicals) subbed to StrepB Select chromagar (CA) (Bio-Rad Laboratories), and the Fast Track Diagnostics GBS real-time PCR (qPCR) assay (Phoenix Airmid Biomedical Corp...
April 26, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28445008/prevalence-of-complement-mediated-cell-lysis-like-gene-sicg-in-streptococcus-dysgalactiae-subsp-equisimilis-isolates-from-japan-2014-2016
#8
Takashi Takahashi, Tomohiro Fujita, Akiyoshi Shibayama, Yuzo Tsuyuki, Haruno Yoshida
BACKGROUND: Streptococcus dysgalactiae subsp. equisimilis (SDSE; a β-hemolytic streptococcus of human or animal origin) infections are emerging worldwide. We evaluated the clonal distribution of complement-mediated cell lysis-like gene (sicG) among SDSE isolates from three central prefectures of Japan. METHODS: Group G/C β-hemolytic streptococci were collected from three institutions from April 2014 to March 2016. Fifty-five strains (52 from humans and three from animals) were identified as SDSE on the basis of 16S rRNA sequencing data...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444868/an-ecological-approach-to-discover-new-bioactive-extracts-and-products-the-case-of-extremophile-plants
#9
Ramla Sahli, Céline Rivière, Christel Neut, Joanne Bero, Marie-Emmanuelle Sahuc, Abderrazak Smaoui, Claire Beaufay, Vincent Roumy, Thierry Hennebelle, Yves Rouillé, Joëlle Quetin-Leclercq, Karin Séron, Riadh Ksouri, Sevser Sahpaz
OBJECTIVES: Eight extremophile plants from Tunisia were screened to find natural products with benefits in human health. METHODS: These plants were collected in different areas in Tunisia. Their methanolic extracts were evaluated for their total phenolic content and for their antiradical (DPPH), antimicrobial (on 35 bacteria and one yeast), antiviral (hepatitis C virus, HCV) and cytotoxic activity (against WI38 and J774 cell lines). The most active species were subjected to a bioguided fractionation...
April 26, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28444596/response-surface-design-for-accumulation-of-selenium-by-different-lactic-acid-bacteria
#10
Jingpeng Yang, Yao Li, Li Zhang, Mingtao Fan, Xinyuan Wei
The accumulation of selenium (Se) by Lactobacillus delbrueckii ssp. bulgaricus (Lb) and Streptococcus thermophilus (St) at the different cultivation conditions, including initial pH, inoculum dose (%), and temperature (°C), was investigated in this work. Se enrichment efficiency was optimized using the Design-Expert software for response surface methodology on a basis of single-factor experiment. The antioxidant activities of Se-enriched Lactic acid bacteria (LAB) were also investigated. The qualitative analysis of Se-enriched LAB was performed by FT-IR spectra...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444395/effects-of-n-acetyl-l-cysteine-on-the-membrane-vesicle-release-and-growth-of-respiratory-pathogens
#11
Charlotte Volgers, Birke J Benedikter, Gert E Grauls, Pauline H M Hellebrand, Paul H M Savelkoul, Frank R M Stassen
Bacterial infections contribute to the disease progression of chronic obstructive pulmonary disease (COPD) by stimulating mucus production in the airways. This increased mucus production and other symptoms are often alleviated when patients are treated with mucolytics such as N-acetyl-L-cysteine (NAC). Moreover, NAC has been suggested to inhibit bacterial growth. Bacteria can release membrane vesicles in response to stress and recent studies report a role for these pro-inflammatory membrane vesicles (MVs) in the pathogenesis of airways disease...
April 21, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28444291/pasteurella-aerogenes-as-an-asymptomatic-bacteriuria-agent
#12
Demet Alaygut, Aynur Engin
'Asymptomatic bacteriuria' (ASB) is isolation of a specified quantitative count of bacteria in an appropriately collected urine specimen obtained from a person without symptoms or signs referable to urinary infection. Catheterized specimens are less likely to be contaminated compared with voided specimens; therefore, positive cultures of catheterized specimens are more likely to reflect true bladder bacteriuria even with low colony counts. The common pathogens for ASB are Escherichia coli, Klebsiella and Streptococcus spp...
April 21, 2017: Journal of Tropical Pediatrics
https://www.readbyqxmd.com/read/28443585/pneumococcal-nasopharyngeal-carriage-in-young-healthy-children-after-pneumococcal-conjugate-vaccine-in-turkey
#13
Ahmet Arvas, Haluk Çokuğraş, Emel Gür, Nevriye Gönüllü, Zeynep Taner, Hrisi Bahar Tokman
AIMS: The purpose of this study was to determine the prevalence of nasopharyngeal carriage of Streptococcus pneumoniae in healthy children aged 0-6 years who were vaccinated with pneumococcal conjugate vaccine. METHODS: The cross-sectional study was conducted on 150 healthy Turkish children aged between 1 month and 6 years of age. Serotyping was performed and risk factors of carriage were evaluated. RESULTS: The overall carriage rate was 14%...
April 13, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28443235/seronegative-complicated-hydatid-cyst-of-the-lung-a-case-report
#14
Eva Letty Susanne Engström, Goran Nadir Salih, Lothar Wiese
Cystic echinococcosis (CE) is an important helminthic zoonotic disease that commonly affects the liver and lungs. Imaging methods and serology establish the diagnosis in most cases. Chest x-ray can diagnose uncomplicated pulmonary hydatid cysts, whereas superinfection and/or rupture of the hydatid cyst (complicated cysts) may change the radiographic appearance and lead to delayed diagnosis and treatment. We report the case of a patient with hemoptysis and chest pain, where computer tomography scan of the lung suggested a large, ruptured hydatid cyst...
2017: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/28443070/crystal-structure-of-glyceraldehyde-3-phosphate-dehydrogenase-from-the-gram-positive-bacterial-pathogen-a-vaginae-an-immunoevasive-factor-that-interacts-with-the-human-c5a-anaphylatoxin
#15
Javier Querol-García, Francisco J Fernández, Ana V Marin, Sara Gómez, Daniel Fullà, Cecilia Melchor-Tafur, Virginia Franco-Hidalgo, Sebastián Albertí, Jordi Juanhuix, Santiago Rodríguez de Córdoba, José R Regueiro, M Cristina Vega
The Gram-positive anaerobic human pathogenic bacterium Atopobium vaginae causes most diagnosed cases of bacterial vaginosis as well as opportunistic infections in immunocompromised patients. In addition to its well-established role in carbohydrate metabolism, D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Streptococcus pyogenes and S. pneumoniae have been reported to act as extracellular virulence factors during streptococcal infections. Here, we report the crystal structure of GAPDH from A. vaginae (AvGAPDH) at 2...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28442909/tat-functionalized-liposomes-for-the-treatment-of-meningitis-an-in-vitro-study
#16
Caterina Bartomeu Garcia, Di Shi, Thomas J Webster
Bacterial meningitis has become a global concern, because of the emergence of antibiotic-resistant bacteria. It has been demonstrated that liposomes can enter bacteria, thus providing a possible treatment for numerous infections, including meningitis. Fusogenic liposomes are pH-sensitive with a high capacity to fuse with the bacteria membrane and promote intracellular drug release. Moreover, this ability can be improved by using cell-penetrating peptides (such as Tat47-57, which is a peptide derived from the Tat protein of HIV)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28442740/deciphering-the-mode-of-action-of-cell-wall-inhibiting-antibiotics-using-metabolic-labeling-of-growing-peptidoglycan-in-streptococcus-pyogenes
#17
Atsushi Sugimoto, Asuka Maeda, Kaori Itto, Hirokazu Arimoto
Because of the scanty pipeline of antibiotics newly obtained from nature, chemical modification of established drugs is one of the major streams of current antibacterial research. Intuitive and easy-to-use assays are critical for identifying drug candidates with novel modes of action. In this study, we demonstrated that metabolic fluorescent staining of growing cell walls is a powerful tool for mode-of-action analyses of antibiotics using Streptococcus pyogenes. A set of major cell-wall-inhibiting antibiotics (bacitracin, D-cycloserine, flavomycin, oxacillin, ramoplanin, and vancomycin) was employed to validate the potential of the assay...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28442416/molecular-characterization-and-expression-of-interleukin-10-and-interleukin-22-in-golden-pompano-trachinotus-ovatus-in-response-to-streptococcus-agalactiae-stimulus
#18
Yinhui Peng, Xiaohui Cai, Guoyin Zhang, Junlin Wang, Yuan Li, Zhiwen Wang, Bei Wang, Xiangying Xiong, Zaohe Wu, Jichang Jian
In the present study, members of the interleukin (IL)-10 family of cytokines, including IL-10 (TOIL-10) and IL-22 (TOIL-22) of golden pompano (Trachinotus ovatus), were cloned for the first time, and their expression patterns and 3D structures analyzed. The full-length cDNA sequences of TOIL-10 and TOIL-22 contained open reading frames of 564 and 567 bp, respectively. TOIL-10 and TOIL-22 shared higher homology (78%-89%) with the corresponding genes from various fish relative to other species (25%-34%) and contained the IL-10 family signature and four cysteine residues that are well conserved in other vertebrate IL-10 members...
April 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28441365/efficacy-and-safety-of-3-nasal-packing-materials-used-after-functional-endoscopic-sinus-surgery-for-chronic-rhinosinusitis-a-comparative-study-in-china
#19
Xi-Ling Zheng, Yu-Xiang Zhao, Min Xu
BACKGROUND This study aimed to investigate the efficacy and safety of 3 nasal packing products (silicone tube, Beschitin-F, and Aquacel1-Ag) after functional endoscopic sinus surgery (FESS) for chronic rhinosinusitis (CRS) patients in China. MATERIAL AND METHODS Sixty-six CRS patients undergoing FESS surgery were randomly divided into 3 groups (22 patients in each group): the silicone tube group, the Beschitin-F group, and the Aquacel1-Ag group. Postoperative headache, nasal pain, nasal bleeding, and swelling of the nasal mucosa were observed at 1 month after FESS surgery...
April 25, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28441350/synthesis-and-bioactivity-evaluation-of-novel-2-salicyloylbenzofurans-as-antibacterial-agents
#20
Phuong-Thuy T Phan, Thu-Trang T Nguyen, Hong-Nhung T Nguyen, Bao-Khanh N Le, Thao T Vu, Dong C Tran, Tuan-Anh N Pham
In order to discover new antibacterial agents, series of 2-salicyloylbenzofuran derivatives were designed, synthesized and evaluated for their antibacterial activities against three Gram-(+) strains (methicillin-sensitive Staphylococcus aureus (MSSA) ATCC 29213, methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300, and Streptococcus faecalis (S. faecalis) ATCC 29212) and one Gram-(-) strain (Escherichia coli (E. coli) ATCC 25922). The 2-salicyloylbenzofuran heterocycles were generated by Rap-Stoermer condensation of salicylaldehydes with phenacyl bromides and then converted to diverse O-ether derivatives by Williamson synthesis...
April 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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