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https://www.readbyqxmd.com/read/29786847/assessment-of-antibiotic-susceptibility-within-lactic-acid-bacteria-and-coagulase-negative-staphylococci-isolated-from-hunan-smoked-pork-a-naturally-fermented-meat-product-in-china
#1
Jing Wang, Xinyuan Wei, Mingtao Fan
The aim of this study was to evaluate the antibiotic susceptibility of lactic acid bacteria (LAB) and coagulase-negative staphylococci (CNS) strains isolated from naturally fermented smoked pork produced in Hunan, China. A total of 48 strains were isolated by selective medium and identified at the species level by 16S rRNA gene sequencing as follows: Staphylococcus carnosus (23), Lactobacillus plantarum (12), Lactobacillus brevis (10), Lactobacillus sakei (1), Weissella confusa (1), and Weissella cibaria (1)...
May 22, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29786766/bacterial-dna-detected-on-pathologically-changed-heart-valves-using-16s-rrna-gene-amplification
#2
Miroslava Chalupova, Anna Skalova, Tomas Hajek, Lenka Geigerova, Dana Kralova, Pavel Liska, Hana Hecova, Jiri Molacek, Jaroslav Hrabak
Nowadays, dental diseases are one of the most common illnesses in the world. Some of them can lead to translocation of oral bacteria to the bloodstream causing intermittent bacteraemia. Therefore, a potential association between oral infection and cardiovascular diseases has been discussed in recent years as a result of adhesion of oral microbes to the heart valves. The aim of this study was to detect oral bacteria on pathologically changed heart valves not caused by infective endocarditis. In the study, patients with pathologically changed heart valves were involved...
May 22, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29786655/bioactive-pyridone-alkaloids-from-a-deep-sea-derived-fungus-arthrinium-sp-ujnmf0008
#3
Jie Bao, Huijuan Zhai, Kongkai Zhu, Jin-Hai Yu, Yuying Zhang, Yinyin Wang, Cheng-Shi Jiang, Xiaoyong Zhang, Yun Zhang, Hua Zhang
Eight new 4-hydroxy-2-pyridone alkaloids arthpyrones D⁻K ( 1 ⁻ 8 ), along with two known analogues apiosporamide ( 9 ) and arthpyrone B ( 10 ), were isolated from a deep-sea-derived fungus Arthrinium sp. UJNMF0008. The structures of the isolated compounds were elucidated on the basis of spectroscopic methods with that of 1 being established by chemical transformation and X-ray diffraction analysis. Compounds 1 and 2 bore an ester functionality linking the pyridone and decalin moieties first reported in this class of metabolites, while 3 and 4 incorporated a rare natural hexa- or tetrahydrobenzofuro[3,2- c ]pyridin-3(2 H )-one motif...
May 22, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29785840/exploring-photoinactivation-of-microbial-biofilms-using-laser-scanning-microscopy-and-confined-two-photon-excitation
#4
Hanna Thomsen, Fabrice E Graf, Anne Farewell, Marica B Ericson
One pertinent complication in bacterial infection is the growth of biofilms, i.e., communities of surface-adhered bacteria resilient to antibiotics. Photodynamic inactivation has been proposed as an alternative to antibiotic treatment; however, novel techniques complementing standard efficacy measures are required. Herein, we present an approach employing multiphoton microscopy complemented with Airyscan super-resolution microscopy, to visualize the distribution of curcumin in Staphylococcus epidermidis biofilms...
May 21, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29785324/-staphylococcus-aureus-is-the-most-common-bacterial-agent-of-the-skin-flora-of-patients-with-seborrheic-dermatitis
#5
Funda Tamer, Mehmet Eren Yuksel, Evren Sarifakioglu, Yavuz Karabag
Background: Seborrheic dermatitis is an inflammatory skin disease that affects 1-3% of the general population. The Malassezia species has been implicated as the main causative agent; however, the bacterial flora of the skin may also play role in the etiopathogenesis. Therefore, we investigated the most common bacterial agent of the skin flora of patients with seborrheic dermatitis. Materials and Methods: Fifty-one patients with seborrheic dermatitis and 50 healthy individuals are included in this study...
April 2018: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/29785216/-staphylococcus-aureus-surface-protein-g-is-an-immunodominant-protein-and-a-possible-target-in-an-anti-biofilm-drug-development
#6
Yury Belyi, Ivan Rybolovlev, Nikita Polyakov, Alena Chernikova, Irina Tabakova, Alexandre Gintsburg
Background: Staphylococcus aureus is a Gram-positive bacterium that causes severe illnesses in the human population. The capacity of S. aureus strains to form biofilms on biotic and abiotic surfaces creates serious problems for treatment of hospital infections and has stimulated efforts to develop new means of specific protection or immunotherapy. Material and Methods: We found that rabbit serum raised against crude concentrated S. aureus liquid culture significantly decreased the development of staphylococcal biofilm in vitro ...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785214/etiological-trends-and-patterns-of-antimicrobial-resistance-in-respiratory-infections
#7
Salma M Al-Zain Ahmed, Sara S Abdelrahman, Doua M Saad, Isra S Osman, Modasir G Osman, Eltahir A G Khalil
Background: Respiratory infections are one of the commonest causes of morbidity and mortality related to infectious diseases worldwide. The emergence of antimicrobial resistance is a major global health problem which is well established in developing countries. Good clinical suspicion and correct laboratory identification of respiratory infection causing organisms followed by the appropriate management are needed to compact both community-acquired and nosocomial infection respiratory infections...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785070/analysis-of-antibiotics-resistant-genes-in-different-strains-of-staphylococcus-aureus
#8
Benson Otarigho, Mofolusho O Falade
The control of Staphylococcus aureus infection is being hampered by methicillin and other resistant strains. The identification of the unique antibiotic resistant genes from the genomes of various strains of S. aureus is of interest. We analyzed 11 S. aureus genomes sequences for Antibiotics Resistance Genes (ARGs) using CARD 2017 platform. We identified 32 ARGs across 11 S. aureus strains. Tet(38), norB, lmrB, mepA and mepR were present across genomes except for S. aureus strain UTSW MRSA 55. The mepA and mepR were found across 11 different genomes...
2018: Bioinformation
https://www.readbyqxmd.com/read/29785029/highly-bactericidal-macroporous-antimicrobial-polymeric-gel-for-point-of-use-water-disinfection
#9
Amit Kumar, Cyrille Boyer, Leena Nebhani, Edgar H H Wong
Access to clean and safe water supply remains inadequate in many developing countries. One of the key challenges is to remove pathogenic bacteria from the water supply via effective water disinfection technologies to prevent the spread of diseases and to ensure the safety of consumers. Herein, a highly effective point-of-use (on-demand) water disinfection technology, in the form of a polymeric scaffold called macroporous antimicrobial polymeric gel (MAPG), is demonstrated. MAPG is easy to fabricate, completely organic and possess inherent antimicrobial property which makes it non-reliant on inorganic compounds such as silver where the long-term toxicity remains unknown...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784985/chronic-rhinosinusitis-with-nasal-polyps-is-characterized-by-dysbacteriosis-of-the-nasal-microbiota
#10
Thanit Chalermwatanachai, Ramiro Vilchez-Vargas, Gabriele Holtappels, Tim Lacoere, Ruy Jáuregui, Frederiek-Maarten Kerckhof, Dietmar H Pieper, Tom Van de Wiele, Mario Vaneechoutte, Thibaut Van Zele, Claus Bachert
Chronic rhinosinusitis with nasal polyp (CRSwNP) patients are often characterized by asthma comorbidity and a type-2 inflammation of the sinonasal mucosa. The mucosal microbiota has been suggested to be implicated in the persistence of inflammation, but associations have not been well defined. To compare the bacterial communities of healthy subjects with CRSwNP patients, we collected nasal swabs from 17 healthy subjects, 21 CRSwNP patients without asthma (CRSwNP-A), and 20 CRSwNP patients with co-morbid asthma (CRSwNP+A)...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784858/production-of-staphylococcal-complement-inhibitor-scin-and-other-immune-modulators-during-the-early-stages-of-staphylococcus-aureus-biofilm-formation-in-a-mammalian-cell-culture-medium
#11
Andi R Sultan, Jasper W Swierstra, Nicole A Lemmens-den Toom, Susan V Snijders, Silvie Hansenová Maňásková, Annelies Verbon, Willem J B van Wamel
Immune modulators are known to be produced by matured biofilms and during different stages of planktonic growth of Staphylococcus aureus. Little is known about immune modulator production during the early stages of biofilm formation, thus raising the question: how does S. aureus protect itself from the innate immune responses at these stages? Therefore, we determined the production of the following immune modulators: chemotaxis inhibitory protein of staphylococci (CHIPS), staphylococcal complement inhibitor (SCIN), formyl peptide receptor-like 1 inhibitor, gamma-hemolysin component B, leukocidin D, E, and S, staphylococcal superantigen-like protein 1, 3, 5 and 9, and staphylococcal enterotoxin A...
May 21, 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29784849/evaluation-of-telavancin-alone-and-combined-with-ceftaroline-or-rifampin-against-methicillin-resistant-staphylococcus-aureus-mrsa-in-an-in-vitro-biofilm-model
#12
Seyedehameneh Jahanbakhsh, Nivedita B Singh, Juwon Yim, Warren E Rose, Michael J Rybak
Biofilm-producing methicillin-resistant Staphylococcus aureus (MRSA) infections are challenging due to increasing antibiotic resistance. Synergistic activity of lipopeptides and lipoglycopeptides with β-lactams has been demonstrated for MRSA, but little is known about biofilm-embedded organisms. Our objective was to evaluate two telavancin (TLV) dosage regimens (7.5 mg/kg and 10 mg/kg q 24h)alone and in combination with ceftaroline (CPT) (600 mg q 8 h) or rifampin (RIF) (450 mg q 12h) against two biofilm-producing MRSA (494 and N315)...
May 21, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29784578/quantitative-study-about-the-role-of-environmental-conditions-in-the-survival-capability-of-multidrug-resistant-bacteria
#13
Adrienn Hanczvikkel, Ákos Tóth
BACKGROUND: Healthcare-associated infections (HAIs) caused by multidrug-resistant bacteria (MDRB) are of global concern and hospital textiles can contribute to their transmission. MDRB are able to survive on textiles for more than enough time to spread in the environment. Some studies summarized the effect of environmental factors on the duration of bacterial survival, but it remained an open question how these factors influence the quantity of surviving bacteria in a period of a few days, which is relevant from the perspective of HAIs...
May 18, 2018: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/29784512/epidemiology-and-etiology-of-invasive-bacterial-infection-in-infants-%C3%A2-60-days-old-treated-in-emergency-departments
#14
Christopher Woll, Mark I Neuman, Christopher M Pruitt, Marie E Wang, Eugene D Shapiro, Samir S Shah, Russell J McCulloh, Lise E Nigrovic, Sanyukta Desai, Adrienne G DePorre, Rianna C Leazer, Richard D Marble, Fran Balamuth, Elana A Feldman, Laura F Sartori, Whitney L Browning, Paul L Aronson
OBJECTIVES: To help guide empiric treatment of infants ≤60 days old with suspected invasive bacterial infection by describing pathogens and their antimicrobial susceptibilities. STUDY DESIGN: Cross-sectional study of infants ≤60 days old with invasive bacterial infection (bacteremia and/or bacterial meningitis) evaluated in the emergency departments of 11 children's hospitals between July 1, 2011 and June 30, 2016. Each site's microbiology laboratory database or electronic medical record system was queried to identify infants from whom a bacterial pathogen was isolated from either blood or cerebrospinal fluid...
May 18, 2018: Journal of Pediatrics
https://www.readbyqxmd.com/read/29784475/post-fabrication-qac-functionalized-thermoplastic-polyurethane-for-contact-killing-catheter-applications
#15
Zachary K Zander, Peiru Chen, Yen-Hao Hsu, Nathan Z Dreger, Laura Savariau, Willie C McRoy, Alec E Cerchiari, Sean D Chambers, Hazel A Barton, Matthew L Becker
The use of catheters is ubiquitous in medicine and the incidence of infection remains unacceptably high despite numerous advances in functional surfaces and drug elution. Herein we report the use of a thermoplastic polyurethane containing an allyl ether side-chain functionality (allyl-TPU) that allows for rapid and convenient surface modification with antimicrobial reagents, post-processing. This post-processing functionalization affords the ability to target appropriate TPU properties and maintain the functional groups on the surface of the device where they do not affect bulk properties...
May 11, 2018: Biomaterials
https://www.readbyqxmd.com/read/29784441/effect-of-methicillin-resistant-staphylococcus-aureus-in-japan
#16
Hironori Uematsu, Kazuto Yamashita, Seiko Mizuno, Susumu Kunisawa, Keigo Shibayama, Yuichi Imanaka
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is the most common antimicrobial-resistant organism identified in Japanese health care facilities. This study analyzed the clinical and economic burdens attributable to methicillin resistance in S aureus in Japanese hospitals. METHODS: We retrospectively investigated data from 14,905 inpatients of 57 hospitals combined with data from nosocomial infection surveillance and administrative claim databases...
May 18, 2018: American Journal of Infection Control
https://www.readbyqxmd.com/read/29784342/rapid-and-sensitive-detection-of-staphylococcus-aureus-assisted-by-polydopamine-modified-magnetic-nanoparticles
#17
Xiaomei Gao, Xin Yao, Zitao Zhong, Li Jia
Pathogens cause significant morbidity and mortality to humans. Thus, development of fast and reliable methods for detection and identification of pathogens is urgently needed to increase protection level of public health and ensure the safety of consumers. Herein, a rapid and sensitive method has been developed for Staphylococcus aureus (S. aureus) detection based on the dual role of polydopamine modified magnetic nanoparticles (PDA@Fe3 O4 NPs) combined with polymerase chain reaction (PCR) and capillary electrophoresis (CE)...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784273/halogenated-trimethoprim-derivatives-as-multidrug-resistant-staphylococcus-aureus-therapeutics
#18
Napon Nilchan, Wanida Phetsang, Taechin Nowwarat, Soraya Chaturongakul, Chutima Jiarpinitnun
Incorporation of halogen atoms to drug molecule has been shown to improve its properties such as enhanced in membrane permeability and increased hydrophobic interactions to its target. To investigate the effect of halogen substitutions on the antibacterial activity of trimethoprim (TMP), we synthesized a series of halogen substituted TMP and tested for their antibacterial activities against global predominant methicillin resistant Staphylococcus aureus (MRSA) strains. Structure-activity relationship analysis suggested a trend in potency that correlated with the ability of the halogen atom to facilitate in hydrophobic interaction to saDHFR...
May 19, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29784040/antimicrobial-resistance-profile-of-staphylococcus-aureus-isolated-from-patients-with-infection-at-tikur-anbessa-specialized-hospital-addis-ababa-ethiopia
#19
Sileshi Tadesse, Haile Alemayehu, Admasu Tenna, Getachew Tadesse, Tefaye Sisay Tessema, Workineh Shibeshi, Tadesse Eguale
BACKGROUND: Staphylococcus aureus is one of the major pathogens of public health importance responsible for various forms of infection. Development of resistance to commonly used antimicrobials limited treatment options against infections due to this pathogen. Antimicrobial resistance profile of Staphylococcus aureus isolated from patients with surgical site infection and ear infection and corresponding nasal swab was investigated in Tikur Anbessa Specialized Hospital (TASH), Addis Ababa, Ethiopia...
May 21, 2018: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/29782697/fewer-defects-in-the-surface-slows-the-hydrolysis-rate-decreases-the-ros-generation-potential-and-improves-the-non-ros-antimicrobial-activity-of-mgo
#20
Nemanja Anicˇić, Marija Vukomanović, Tilen Koklicˇ, Danilo Suvorov
Magnesium oxide (MgO) is recognised as exhibiting a contact-based antibacterial activity. However, a comprehensive study of the impact of atomic-scale surface features on MgO's antibacterial activity is lacking. In this study, the nature and abundance of the native surface defects on different MgO powders are thoroughly investigated. Their impacts on the hydrolysis kinetics, antibacterial activity against Escherichia coli (ATCC 47076), Staphylococcus epidermidis and Pseudomonas aeruginosa and the reactive oxygen species (ROS) generation potential are determined and explained...
May 21, 2018: Small
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