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Gerardo Víquez-Molina, Javier Aragón-Sánchez, Cristian Pérez-Corrales, Christian Murillo-Vargas, María Eugenia López-Valverde, Benjamin A Lipsky
The aim of this study is to describe the presence of genes encoding for 4 virulence factors (pvl, eta, etb, and tsst), as well as the mecA gene conferring resistance to beta-lactam antibiotics, in patients with diabetes and a staphylococcal foot infection. We have also analyzed whether isolates of Staphylococcus aureus from bone infections have a different profile for these genes compared with those from exclusively soft tissue infections. In this cross-sectional study of a prospectively recruited series of patients admitted to the Diabetic Foot Unit, San Juan de Dios Hospital, San José, Costa Rica with a moderate or severe diabetic foot infection (DFI), we collected samples from infected soft tissue and from bone during debridement...
March 1, 2018: International Journal of Lower Extremity Wounds
Zarizal Suhaili, Putri 'Amira Rafee, Norhidayah Mat Azis, Chew Chieng Yeo, Syafinaz Amin Nordin, Abdul Rachman Abdul Rahim, Mazen M Jamil Al-Obaidi, Mohd Nasir Mohd Desa
Introduction: This study aims to assess the antimicrobial susceptibility profiles of Staphylococcus aureus strains isolated from university students and to determine the prevalence of constitutive and inducible clindamycin resistance, the latter being able to cause therapeutic failure due to false in vitro clindamycin susceptibility. Methods: S. aureus strains were isolated from the nasal swabs of 200 health sciences students of a Malaysian university. Twelve classes of antibiotics were used to evaluate the antimicrobial susceptibility profiles with the macrolide-lincosamide-streptogramin B (MLSB ) phenotype for inducible clindamycin resistance determined by the double-diffusion test (D-test)...
March 2018: Germs
Jinxiao Guo, Huaijuan Zhou, Jiaxing Wang, Wei Liu, Mengqi Cheng, Xiaochun Peng, Hui Qin, Jianfeng Wei, Ping Jin, Jinhua Li, Xianlong Zhang
Vanadium is a trace element in the human body, and vanadium compounds have a promising future in biological and medical applications due to their various biological activities and low toxicity. Herein, a novel pure vanadium dioxide (VO2 ) nanofilm was deposited on a substrate of biomedical titanium by magnetron sputtering. The antibacterial effect of VO2 against the methicillin-resistant Staphylococcus aureus (MRSA) was validated in vitro and in vivo. Moreover, the biocompatibility of VO2 and its osteogenic effects were systematically illustrated...
March 21, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Takeshi Yamada, Miu Yagita, Yutaka Kobayashi, Goh Sennari, Hiroyuki Shimamura, Hidehito Matsui, Yuki Horimatsu, Hideaki Hanaki, Tomoyasu Hirose, Satoshi Omura, Toshiaki Sunazuka
Total synthesis of bottromycin A2 can be accomplished through a diastereoselective Mannich reaction of a chiral sulfinamide, mercury-mediated intermolecular amidination, and cyclization of a constrained tetracyclic peptide. Exploitation of this process allowed the synthesis of several novel bottromycin analogs. The antimicrobial activity of these analogs was evaluated in vitro against Gram-positive bacteria, such as methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE)...
March 21, 2018: Journal of Organic Chemistry
Gabriela Ensinck, Adriana Ernst, Gustavo Lazarte, Antonela Romagnoli, Yanina Sguassero, Nanci Míguez, Santiago López Papucci, Alicia Aletti, Ana Chiossone, Fernanda Pigozzi, Matías Pinotti, Ana Cantador
INTRODUCTION: Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are a common reason for consultation in pediatrics. Most of them present as skin and soft tissue infections; however, invasive infections have increased during the last decade. OBJECTIVES: The main objective was to describe the clinical-epidemiological characteristics of CA-MRSA infections. The secondary objective was to compare prevalence, clinical presentation and antibiotic susceptibility with a pre-study period (1/2004-12/2007)...
April 1, 2018: Archivos Argentinos de Pediatría
Silje B Jørgensen, Nina Handal, Kaja Linn Fjeldsæter, Lars Kåre Kleppe, Torni Myrbakk, Dorthea Hagen Oma, Yngvar Tveten, Mette Walberg, Grethe Ørnevik
BAKGRUNN: I norsk helsevesen gjennomføres omfattende tiltak for å hindre spredning av meticillinresistente Staphylococcus aureus (MRSA). Vi ønsket å undersøke hvor mange smitteoppsporinger som gjøres rundt nyoppdagede MRSA-tilfeller hos pasienter og ansatte i sykehus, og hvor ofte smitteoppsporingene fører til ytterligere funn hos helsepersonell. MATERIALE OG METODE: I denne retrospektive observasjonsstudien bidro smittevernenhetene ved åtte helseforetak i landets fire helseregioner med opplysninger om MRSA-funn hos helsepersonell etter gjennomførte MRSA-smitteoppsporinger...
March 19, 2018: Tidsskrift for Den Norske Lægeforening: Tidsskrift for Praktisk Medicin, Ny Række
Jon Birger Haug
No abstract text is available yet for this article.
March 19, 2018: Tidsskrift for Den Norske Lægeforening: Tidsskrift for Praktisk Medicin, Ny Række
Maria Pardos de la Gandara, Vitor Borges, Marilyn Chung, Catarina Milheiriço, João Paulo Gomes, Herminia de Lencastre, Alexander Tomasz
Methicillin-resistant Staphylococcus aureus (MRSA) strains carry either a mecA - or a mecC -mediated mechanism of resistance to beta-lactam antibiotics and the phenotypic expression of resistance shows extensive strain-to-strain variation. In recent communications we identified genetic determinants associated with the stringent stress response that play a major role in the antibiotic resistant phenotype of the historically earliest "archaic" clone of MRSA and in the mecC carrying MRSA strain LGA251...
March 19, 2018: Antimicrobial Agents and Chemotherapy
Kieu-Nhi Tran, Michael J Rybak
Background: Increasing utilization of vancomycin due to the high prevalence of methicillin-resistant S. aureus (MRSA) infections has lead to the emergence of vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA). In vitro data suggest the potential for potent synergy between several beta-lactams and vancomycin. The objective of this study is to evaluate the synergy between beta-lactams and vancomycin against MRSA that is vancomycin susceptible, vancomycin susceptible Staphylococcus aureus (VSSA), hVISA, and VISA...
March 19, 2018: Antimicrobial Agents and Chemotherapy
Andrew M Stoessel, Cory M Hale, Robert W Seabury, Christopher D Miller, Jeffrey M Steele
OBJECTIVE: This study aimed to assess the impact of area under the curve (AUC)-based vancomycin monitoring on pharmacist-initiated dose adjustments after transitioning from a trough-only to an AUC-based monitoring method at our institution. METHODS: A retrospective cohort study of patients treated with vancomycin for complicated methicillin-resistant Staphylococcus aureus (MRSA) infection between November 2013 and December 2016 was conducted. The frequency of pharmacist-initiated dose adjustments was assessed for patients monitored via trough-only and AUC-based approaches for trough ranges: 10 to 14...
January 1, 2018: Journal of Pharmacy Practice
Jing Li, Wei-Song Kong, Xin Liu, Yong-Qin Geng, Jin Wang, Yong Xu, Xue-Mei Li, Guang-Yu Yang, Min Zhou, Qiu-Fen Hu, Tao Li, Ci-Qing Jiang
A new isoflavone derivative was isolated from Rosa damascena by using various chromatographic techniques including silica gel, Sephadex LH-20, and preparative RP-HPLC separation. Its structure was identified as 4'-hydroxy-7-(3-hydroxypropanoyl)-6-methoxy-isoflavone using combined examinations of their UV, IR, MS, and NMR spectroscopic data. Biological activity test showed that this compound showed prominent antibacterial activity with MIC₉₀ value of (46±4) mg·L⁻¹ for methicillin resistant Staphylococcus aureus(MRSA) strain...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Juliana S Novais, Caroline S Moreira, Ana Carolina J A Silva, Raquel S Loureiro, Agnes Marie Sá Figueiredo, Vitor F Ferreira, Helena C Castro, David R da Rocha
The aims of this study were the planning, synthesis and in vitro evaluation of 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against Gram-negative and Gram-positive strains, searching for potential lead compounds against bacterial biofilm formation. A series of 12 new analogs of 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones were synthesized by adding a thiol and different substituents to a ο-quinone methide using microwave irradiation. The compounds were tested against Gram-positive (Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, S...
March 14, 2018: Microbial Pathogenesis
H Morioka, M Nagao, S Yoshihara, H Ohge, K Kasahara, N Shigemoto, T Kajihara, M Mori, M Iguchi, Y Tomita, S Ichiyama, T Yagi
BACKGROUND: The Japanese government adopted a national action plan on antimicrobial resistance, which aims to reduce drug-resistant pathogens and antimicrobial use. A point-prevalence survey (PPS) is a useful surveillance method to gain information about hospital epidemiology; however, no multi-centre PPS has previously been performed in Japan. AIM: To investigate general information about hospital epidemiology, healthcare-associated infections (HCAIs), and antimicrobial use in multiple Japanese university hospitals...
March 13, 2018: Journal of Hospital Infection
Taisa Trevizani Rocchetti, Katheryne Benine Martins, Patricia Yoshida Faccioli Martins, Rogério Antonio de Oliveira, Alessandro Lia Mondelli, Carlos Magno Castelo Branco Fortaleza, Maria de Lourdes Ribeiro de Souza da Cunha
INTRODUCTION: Staphylococcus spp. - both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) - are relevant agents of healthcare-associated infections. Therefore, the rapid recognition of MRSA and methicillin-resistant CoNS from blood stream infections is critically important for patient management. It is worth noting that inappropriate empiric therapy has been associated with higher in-hospital mortality. MATERIAL AND METHODS: In this study we evaluated a multiplex PCR standardized to detect Staphylococcus spp...
March 13, 2018: Brazilian Journal of Infectious Diseases
Mercedes Pérez-Bonilla, Daniel Oves-Costales, Mercedes de la Cruz, Maria Kokkini, Jesús Martín, Francisca Vicente, Olga Genilloud, Fernando Reyes
Phocoenamicins B and C ( 1 and 2 ), together with the known spirotetronate phocoenamicin ( 3 ), were isolated from cultures of Micromonospora sp. The acetone extract from a culture of this strain, isolated from marine sediments collected in the Canary Islands, displayed activity against methicillin-resistant Staphylococcus aureus (MRSA), Mycobacterium tuberculosis H37Ra and Mycobacterium bovis . Bioassay-guided fractionation of this extract using SP207ss column chromatography and preparative reversed-phased HPLC led to the isolation of the new compounds 1 and 2 belonging to the spirotetronate class of polyketides...
March 16, 2018: Marine Drugs
Khaled R Alkharsah, Suriya Rehman, Fatimah Alkhamis, Amani Alnimr, Asim Diab, Amein K Al-Ali
BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) constitutes a major global health concern causing hospital and community acquired infections. A wide diversity of MRSA genotypes are circulating in geographically related regions. Therefore understanding the molecular epidemiology of MRSA is fundamental to design control and clearance measures. METHODS: A total of 106 MRSA isolates from infection (51) and carrier colonization sites (55) are characterized genetically based on SCCmec and MLST genotyping methods in addition to detection of PVL, TSST-1 and enterotoxins...
March 15, 2018: Annals of Clinical Microbiology and Antimicrobials
Albert Boada, Mariona Pons-Vigués, Jordi Real, Elisabet Grezner, Bonventura Bolíbar, Carl Llor
INTRODUCTION: Commensal flora of healthy people is becoming an important reservoir of resistant bacteria. OBJECTIVES: To evaluate the relationship of previous antibiotic-dispensed and resistance pattern of strains of Staphylococcus aureus in primary care patients. METHODS: A cross-sectional study was carried out in seven primary care centres in Catalonia, Spain, from October 2010 to May 2011, as part of the APRES (The appropriateness of prescribing antibiotics in primary care in Europe concerning antibiotic resistance) study...
December 2018: European Journal of General Practice
Shuaishuai Ni, Baoli Li, Feifei Chen, Hanwen Wei, Fei Mao, Yifu Liu, Yixiang Xu, Xiaoxi Qiu, Xiaokang Li, Wenwen Liu, Linghao Hu, Dazheng Ling, Manjiong Wang, Xinyu Zheng, Jin Zhu, Lefu Lan, Jian Li
Diapophytoene desaturase (CrtN) is a potential novel target for intervening in the biosynthesis of the virulence factor staphyloxanthin. In this study, 38 1,4-benzodioxan-derived CrtN inhibitors were designed and synthesized to overwhelm the defects of leading compound 4a . Derivative 47 displayed superior pigment inhibitory activity, better hERG inhibitory properties and water solubility, and significantly sensitized MRSA strains to immune clearance in vitro. Notably, 47 displayed excellent efficacy against pigmented S...
March 8, 2018: ACS Medicinal Chemistry Letters
Hannah Y P Phoon, Hazilawati Hussin, Baizurah Mohd Hussain, Shu Yong Lim, Jia Jie Woon, Yi Xian Er, Kwai Lin Thong
OBJECTIVES: Hospital environments are potential reservoirs of bacteria associated with nosocomial infections. Here, we determined the distribution of cultivable environmental bacteria of clinical importance from a Malaysian tertiary hospital and to investigate their resistotypes and genotypes. METHODS: Swab and fluid samples (n=358) from healthcare workers' hands, frequently touched surfaces, medical equipment, patients' immediate surroundings, ward sinks and toilets and solutions or fluids of 12 selected wards were collected...
March 11, 2018: Journal of Global Antimicrobial Resistance
Luís Belo, Isa Serrano, Eva Cunha, Carla Carneiro, Luis Tavares, L Miguel Carreira, Manuela Oliveira
BACKGROUND: Most of surgical site infections (SSI) are caused by commensal and pathogenic agents from the patient's microbiota, which may include antibiotic resistant strains. Pre-surgical asepsis of the skin is one of the preventive measures performed to reduce SSI incidence and also antibiotic resistance dissemination. However, in veterinary medicine there is no agreement on which biocide is the most effective. The aim of this study was to evaluate the effectiveness of two pre-surgical skin asepsis protocols in dogs...
March 14, 2018: BMC Veterinary Research
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