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https://www.readbyqxmd.com/read/28330289/progress-in-genome-sequencing-will-accelerate-molecular-breeding-in-cotton-gossypium-spp
#1
REVIEW
Rong Yan, Chengzhen Liang, Zhigang Meng, Waqas Malik, Tao Zhu, Xuefeng Zong, Sandui Guo, Rui Zhang
Cotton (Gossypium spp.) is the single most important spinning fiber that has economic significance worldwide. Cotton is one of the most value-added crops and an excellent model system for the analysis of polyploidization and cell development. Thus, the Cotton Genome Consortium has made rapid and significant progress in whole genome sequencing studies in the last decade. Developments in cotton genome sequencing and assembly provide powerful tools for dissecting the genetic and molecular bases of agronomically important traits and establishing regulatory networks on these processes, which leads to molecular breeding...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330232/high-quality-metagenomic-dna-from-marine-sediment-samples-for-genomic-studies-through-a-preprocessing-approach
#2
Solly Solomon, Bhavya Kachiprath, G Jayanath, T P Sajeevan, I S Bright Singh, Rosamma Philip
Recent advances in culture-independent studies of microbes had proved to be more reliable and efficient than the conventional ones. The isolation of good quality and quantity of total community DNA are one of the major hurdles in this endeavour. Shearing of DNA during the extraction process and the co-extraction of inhibitory compounds reduce the quality of the isolated nucleic acids making it unsuitable for the construction of large insert metagenomic libraries. In the present study, a multi-level filtration step was brought in which efficiently isolated total bacterial DNA from three different environment samples...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330193/a-rapid-method-for-isolation-of-genomic-dna-from-food-borne-fungal-pathogens
#3
S Umesha, H M Manukumar, Sri Raghava
Food contaminated with fungal pathogens can cause extremely harmful effects to human even when present in low concentrations. Researchers now pay more attention towards rapid DNA extraction for the quick screening, which is highly demanded in diverse research field. Molecular description of many fungal species is identified by different molecular characteristics. Hence, the efficient isolation of genomic DNA and amplification using PCR is a prerequisite for molecular characterization. Here, we used an improved Sodium dodecyl sulfate-Cetyltrimethyl ammonium bromide-Chloroform-isoamyl alcohol method by combining Sodium dodecyl sulfate with cetyl methylammonium bromide without addition of proteinase K, RNase K, and β-mercaptoethanol...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330186/a-comprehensive-study-of-one-step-selenization-process-for-cu-in1-x-ga-x-se2-thin-film-solar-cells
#4
Shih-Chen Chen, Sheng-Wen Wang, Shou-Yi Kuo, Jenh-Yih Juang, Po-Tsung Lee, Chih Wei Luo, Kaung-Hsiung Wu, Hao-Chung Kuo
In this work, aiming at developing a rapid and environmental-friendly process for fabricating CuIn1-x Ga x Se2 (CIGS) solar cells, we demonstrated the one-step selenization process by using selenium vapor as the atmospheric gas instead of the commonly used H2Se gas. The photoluminescence (PL) characteristics indicate that there exists an optimal location with superior crystalline quality in the CIGS thin films obtained by one-step selenization. The energy dispersive spectroscopy (EDS) reveals that the Ga lateral distribution in the one-step selenized CIGS thin film is intimately correlated to the blue-shifted PL spectra...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28330170/an-efficient-and-cost-effective-method-of-rna-extraction-from-mucilage-phenol-and-secondary-metabolite-rich-bark-tissue-of-tossa-jute-c-olitorius-l-actively-developing-phloem-fiber
#5
S B Choudhary, M Kumar, I Chowdhury, R K Singh, S P Pandey, H K Sharma, P G Karmakar
Tossa jute is an important natural fiber crop of Southeast Asian countries including India, Bangladesh, China, Thailand, Myanmar etc. Traditional industrial application of jute fiber is limited to the packaging products like hessians, sacks, etc. and the fiber found unsuitable for textile industries largely due to significantly high lignin content. Therefore, understanding genetic factors underlying lignin biosynthesis in tossa jute holds promise for jute based product diversification. The major limiting factor in undertaking such study is unavailability of efficient protocol for RNA extraction at secondary growth active stage of tossa jute...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330167/genetic-characterization-of-moroccan-and-the-exotic-bread-wheat-cultivars-using-functional-and-random-dna-markers-linked-to-the-agronomic-traits-for-genomics-assisted-improvement
#6
Fatima Henkrar, Jamal El-Haddoury, Hassan Ouabbou, Najib Bendaou, Sripada M Udupa
Genetic characterization, diversity analysis and estimate of the genetic relationship among varieties using functional and random DNA markers linked to agronomic traits can provide relevant guidelines in selecting parents and designing new breeding strategies for marker-assisted wheat cultivar improvement. Here, we characterize 20 Moroccan and 19 exotic bread wheat (Triticum aestivum L.) cultivars using 47 functional and 7 linked random DNA markers associated with 21 loci of the most important traits for wheat breeding...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329895/using-a-state-birth-registry-as-a-quality-improvement-tool
#7
Carole Lannon, Heather C Kaplan, Kelly Friar, Sandra Fuller, Susan Ford, Beth White, John Besl, John Paulson, Michael Marcotte, Michael Krew, Jennifer Bailit, Jay Iams
Background Birth registry data are universally collected, generating large administrative datasets. However, these data are typically not used for quality improvement (QI) initiatives in perinatal medicine because the quality and timeliness of the information is uncertain. Objective We sought to identify and address causes of inaccuracy in recording birth registry information so that birth registry data could support statewide obstetrical quality initiatives in Ohio. Study Design The Ohio Perinatal Quality Collaborative and the Ohio Department of Health Vital Statistics used QI techniques in 15 medium-sized maternity hospitals to identify and remove systemic sources of inaccuracy in birth registry data...
March 22, 2017: American Journal of Perinatology
https://www.readbyqxmd.com/read/28329867/effectiveness-of-a-virtual-reality-forest-on-people-with-dementia-a-mixed-methods-pilot-study
#8
Wendy Moyle, Cindy Jones, Toni Dwan, Tanya Petrovich
Purpose of the Study: To measure and describe the effectiveness of a Virtual Reality Forest (VRF) on engagement, apathy, and mood states of people with dementia, and explore the experiences of staff, people with dementia and their families. Design and Methods: A mixed-methods study conducted between February and May 2016. Ten residents with dementia, 10 family members, and 9 care staff were recruited from 2 residential aged care facilities, operated by one care provider, located in Victoria, Australia...
March 15, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329865/what-doesn-t-kill-you-doesn-t-make-you-stronger-the-long-term-consequences-of-nonfatal-injury-for-older-adults
#9
Dongjuan Xu, Julia A Rivera Drew
Purpose: The majority of research efforts centering on injury among older adults focus on fall-related injuries and short-term consequences of injury. Little is known about the long-term consequences of all-cause nonfatal injuries, including minor injuries. Using a recent, large, and nationally representative sample of the U.S. non-institutionalized civilian population, the current study examines whether older adults who sustained a nonfatal injury (serious and minor) have higher risk of long-term morbidity and mortality outcomes compared with noninjured seniors...
March 10, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329849/development-and-inter-rater-reliability-of-the-mealtime-scan-for-long-term-care
#10
Heather H Keller, Habib Chaudhury, Kaylen J Pfisterer, Susan E Slaughter
Background and Objectives: Poor food intake is common in long-term care (LTC). The mealtime experience has been identified as influential, yet, research has been limited by lack of quality, standardized measures. The purpose of this study was to develop and test for inter-rater reliability the Mealtime Scan (MTS), an observational measure. Research Design and Methods: MTS was derived from the literature on ambiance, mealtime experience, social interactions at mealtimes, and social models of care...
March 15, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329753/benefits-of-high-intensity-exercise-training-to-patients-with-chronic-obstructive-pulmonary-disease-a-controlled-study
#11
Tobias Boeselt, Christoph Nell, Lea Lütteken, Katharina Kehr, Janine Koepke, Sandra Apelt, Martina Veith, Björn Beutel, Marc Spielmanns, Timm Greulich, Claus F Vogelmeier, Klaus Kenn, Sabina Janciauskiene, Peter Alter, A Rembert Koczulla
BACKGROUND: Various exercise training programs are used for patients with chronic obstructive pulmonary disease (COPD) of different severity. OBJECTIVES: To investigate the impact of individualized high-intensity training on exercise capacity with COPD. METHODS: A total of 49 patients agreed to participate. Of these, 31 were assigned to the training group and 18 served as controls. The training group exercised twice a week for 90 min with consecutively increasing loads...
March 23, 2017: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/28329709/potential-impacts-of-changing-supply-water-quality-on-drinking-water-distribution-a-review
#12
REVIEW
Gang Liu, Ya Zhang, Willem-Jan Knibbe, Cuijie Feng, Wentso Liu, Gertjan Medema, Walter van der Meer
Driven by the development of water purification technologies and water quality regulations, the use of better source water and/or upgraded water treatment processes to improve drinking water quality have become common practices worldwide. However, even though these elements lead to improved water quality, the water quality may be impacted during its distribution through piped networks due to the processes such as pipe material release, biofilm formation and detachment, accumulation and resuspension of loose deposits...
March 19, 2017: Water Research
https://www.readbyqxmd.com/read/28329681/sirt6-promotes-dna-end-joining-in-ipscs-derived-from-old-mice
#13
Wen Chen, Nana Liu, Hongxia Zhang, Haiping Zhang, Jing Qiao, Wenwen Jia, Songcheng Zhu, Zhiyong Mao, Jiuhong Kang
Induced pluripotent stem cells (iPSCs) have great potential for treating age-related diseases, but the genome integrity of iPSCs is critically important. Here, we demonstrate that non-homologous end joining (NHEJ), rather than homologous recombination (HR), is less efficient in iPSCs from old mice than young mice. We further find that Sirt6 is downregulated in iPSCs from old mice. Sirt6 directly binds to Ku80 and facilitates the Ku80/DNA-PKcs interaction, thus promoting DNA-PKcs phosphorylation at residue S2056, leading to efficient NHEJ...
March 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/28329638/developing-a-continuous-quality-improvement-assessment-using-a-patient-centered-approach-in-optimizing-sle-disease-control
#14
Katelyn Updyke, Brittany Urso, James A Solomon
Systemic lupus erythematous is a multi-organ autoimmune disease in which the patient has "lost" self- tolerance and developed immune-complexes containing self-antigen that cause disease through tissue inflammation and multi-organ damage. Patients often have unpredictable flares in symptoms with poorly identified triggers. However, it has been suggested in other literature that exogenous exposures, including ingested beef, cigarette smoke, UV radiation, metal-complexing agents, and infections can contribute to exacerbations and improvements in SLE symptoms...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329634/comparing-survival-of-patients-with-single-or-multiple-primary-melanoma-in-the-netherlands-1994-2009
#15
L M Pardo, R J T Ven der Leest, E De Vries, I Soerjomataram, T Nijsten, L M Hollestein
There is conflicting literature as to whether the survival of patients with multiple melanoma is worse that this of single melanoma cases. We used extended Cox-proportional hazard models to estimate hazard-ratios (HR) of patients with multiple melanomas against single melanomas. Data from the nationwide Dutch Cancer Registry was used to retrieve cutaneous melanoma cases between 1994 and 2009 and linked with municipal records to assess the vital status. To account for the longer survival in the multiple melanoma cases we modelled multiple melanoma as time-varying variable, as well as Breslow thickness, histological subtype and nodal/distant metastasis...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329439/clinical-intuition-in-the-nursing-process-and-decision-making-a-mixed-studies-review
#16
REVIEW
Christina Melin-Johansson, Rebecca Palmqvist, Linda Rönnberg
AIM AND OBJECTIVES: To review what is characteristic of registered nurses' intuition in clinical settings, in relationships, and in the nursing process. BACKGROUND: Intuition is a controversial concept and nurses believe that there are difficulties in how they should explain their nursing actions or decisions based on intuition. Much of the evidence from the body of research indicates that nurses value their intuition in a variety of clinical settings. More information on how nurses integrate intuition as a core element in daily clinical work would contribute to an improved understanding on how they go about this...
March 22, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/28329420/towards-a-symptom-cluster-model-in-chronic-kidney-disease-a-structural-equation-approach
#17
Hayfa Almutary, Clint Douglas, Ann Bonner
AIMS: To test a symptom cluster model in chronic kidney disease patients based on the Theory of Unpleasant Symptoms, accounting for the relationships between influencing factors, symptom experience and consequences for quality of life. BACKGROUND: The evaluation of symptom clusters is a new field of scientific inquiry directed toward more focused symptom management. Yet, little is known about relationships between symptom clusters, predictors and the synergistic effect of multiple symptoms on outcomes...
March 22, 2017: Journal of Advanced Nursing
https://www.readbyqxmd.com/read/28329412/measuring-flow-rate-in-crystalline-bedrock-wells-using-the-dissolved-oxygen-alteration-method
#18
Sarah A Vitale, Gary A Robbins
Determination of vertical flow rates in a fractured bedrock well can aid in planning and implementing hydraulic tests, water quality sampling, and improving interpretations of water quality data. Although flowmeters are highly accurate in flow rate measurement, the high cost and logistics may be limiting. In this study the dissolved oxygen alteration method (DOAM) is expanded upon as a low-cost alternative to determine vertical flow rates in crystalline bedrock wells. The method entails altering the dissolved oxygen content in the wellbore through bubbler aeration, and monitoring the vertical advective movement of the dissolved oxygen over time...
March 22, 2017: Ground Water
https://www.readbyqxmd.com/read/28329395/long-term-symptom-improvement-and-patient-satisfaction-following-catheter-ablation-of-supraventricular-tachycardia-insights-from-the-german-ablation-registry
#19
Johannes Brachmann, Thorsten Lewalter, Karl-Heinz Kuck, Dietrich Andresen, Stephan Willems, Stefan G Spitzer, Florian Straube, Burghard Schumacher, Lars Eckardt, Dejan Danilovic, Dierk Thomas, Matthias Hochadel, Jochen Senges
Aims: To analyse outcomes of supraventricular tachycardia (SVT) ablations performed within a prospective German Ablation Quality Registry. Methods and results: Data from 12 566 patients who underwent catheter ablation of SVT between January 2007 and January 2010 to treat atrial fibrillation (AFIB, 37.2% of procedures), atrial flutter (AFL, 29.9%), atrioventricular nodal re-entrant tachycardia (AVNRT, 23.2%), atrioventricular re-entrant tachycardia (6.3%), and focal atrial tachycardia (AT, 3...
March 17, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28329279/performance-of-hospitals-according-to-the-esc-acca-quality-indicators-and-30-day-mortality-for-acute-myocardial-infarction-national-cohort-study-using-the-united-kingdom-myocardial-ischaemia-national-audit-project-minap-register
#20
Owen Bebb, Marlous Hall, Keith A A Fox, Tatendashe B Dondo, Adam Timmis, Hector Bueno, François Schiele, Chris P Gale
Aims: To investigate the application of the European Society of Cardiology Acute Cardiovascular Care Association quality indicators (QI) for acute myocardial infarction for the study of hospital performance and 30-day mortality. Methods and results: National cohort study (n = 118,075 patients, n = 211 hospitals, MINAP registry), 2012-13. Overall, 16 of the 20 QIs could be calculated. Eleven QIs had a significant inverse association with GRACE risk adjusted 30-day mortality (all P < 0...
February 20, 2017: European Heart Journal
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