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https://www.readbyqxmd.com/read/29054741/virucidal-efficacy-of-food-additive-grade-calcium-hydroxide-against-surrogate-of-human-norovirus
#1
Natthanan Sangsriratanakul, Chiharu Toyofuku, Mayuko Suzuki, Miyuki Komura, Masashi Yamada, Md Shahin Alam, Sakchai Ruenphet, Dany Shoham, Kouji Sakai, Kazuaki Takehara
An alkaline agent, namely, food additive grade calcium hydroxide (FdCa(OH)2) in the solution, powder and suspension forms was evaluated as a virucidal agent, using a murine norovirus (MNV) as the surrogate for human norovirus. The main constituent of FdCa(OH)2 is Ca(OH)2, which has pH 13 in 0.17% solution. The results showed that 0.17% FdCa(OH)2 solution could inactivate MNV within 30sec even in the presence of organic materials (5% fetal bovine serum (FBS)). In a contaminated surface experiment, MNV with 5% FBS was inoculated on rayon sheets, and the result showed FdCa(OH)2 solution could markedly reduce virus titer within 1min...
October 17, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/29054608/a-sweet-spot-for-fluid-induced-oscillations-in-the-conditioning-of-stem-cell-based-engineered-heart-valve-tissues
#2
Alexander Williams, Sana Nasim, Manuel Salinas, Arash Moshkforoush, Nikolaos Tsoukias, Sharan Ramaswamy
Fluid-induced shear stresses are involved in the development of cardiovascular tissues. In a tissue engineering framework, this stimulus has also been considered as a mechanical regulator of stem cell differentiation. We recently demonstrated that the fluid-oscillating effect in combination with a physiologically-relevant shear stress magnitude contributes to the formation of stem cell-derived de novo heart valve tissues. However, the range of oscillations necessary to induce favorable gene expression and engineered tissue formation is unknown...
October 7, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29054069/postmortem-distribution-of-chlorpyrifos-methyl-fenitrothion-and-their-metabolites-in-body-fluids-and-organ-tissues-of-an-intoxication-case
#3
Tatsunori Takayasu, Hiroki Yamamoto, Yuko Ishida, Mizuho Nosaka, Mariko Kawaguchi, Yumi Kuninaka, Akihiko Kimura, Toshikazu Kondo
We herein report a fatal intoxication case caused by the ingestion of the insecticides chlorpyrifos-methyl (CPFM) and fenitrothion (MEP). A 70-year-old man was found dead in his house and a cup containing a small amount of agricultural chemicals was on the table near his body. External and internal examinations revealed no injuries. In a gas chromatography-mass spectrometry (GC-MS) screening test, CPFM, MEP, and their metabolites, 3,5,6-trichloro-2-pyridinol (TCPY) and 3-methyl-4-nitrophenol (3MNP), respectively, were qualitatively detected in his stomach contents...
October 4, 2017: Legal Medicine
https://www.readbyqxmd.com/read/29053858/ectoparasitism-of-commercial-laying-hen-by-megninia-ginglymura-m%C3%A3-gnin-acari-population-dynamic-and-distribution-on-the-body-regions
#4
Tamara B Horn, Matheus S Rocha, Juliana Granich, Júlia H Körbes, Luis F A Alves, Noeli J Ferla
Ectoparasites are temporary or permanent skin dwellers. Megninia ginglymura (Mégnin) (Analgidae) causes economic damage in commercial poultry farms as a result of lower egg production or even death of the host. Little is known about Megninia ginglymura's life cycle and infestation. This study aimed to evaluate the preference of M. ginglymura for different body regions of the host Gallus gallus L. and its abundance and population dynamics in different laying hen houses. Samples were collected from August 2013 to August 2014 in three different commercial laying hen systems: automatic production systems (A1,2,3); semiautomatic systems (S1 (free of pesticides) and S2) and free-range system (FR)...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053839/laying-hens-behave-differently-in-artificially-and-naturally-sourced-ammoniated-environments
#5
B B Pokharel, V M Dos Santos, D Wood, B Van Heyst, A Harlander-Matauschek
Laying hens are chronically exposed to high levels of ammonia (NH3), one of the most abundant aerial pollutants in poultry houses. Tests for aversion to NH3 in laying hens have used artificially sourced NH3/air mixtures (i.e., from a gas cylinder) showing that birds prefer fresh air to NH3. However, artificially sourced NH3/air mixtures may not accurately reflect barn air conditions, where manure emits a variety of gases. Herein, we investigated whether laying hens differentiate between artificially and naturally sourced NH3/air mixtures and how exposure to NH3 affects foraging and aversive behavior...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053822/characterization-of-indigenous-chicken-production-systems-in-rwanda
#6
J Mahoro, T K Muasya, F Mbuza, R Habimana, A K Kahi
Characterization of indigenous chicken (IC) production systems in Rwanda was conducted from November 2015 to January 2016 with the aim of understanding socio-economic characteristics, management of IC, production parameters, feed resources, and constraints faced by farmers rearing IC. A survey involving 206 households was carried out in 5 districts with the highest populations of IC using structured questionnaires. Data were analyzed using SAS software. The results showed that the majority of respondents (62...
September 14, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053818/effect-of-dietary-supplementation-of-nitrocompounds-on-salmonella-colonization-and-ileal-immune-gene-expression-in-laying-hens-challenged-with-salmonella-enteritidis
#7
Pratima Adhikari, D E Cosby, N A Cox, W K Kim
Foodborne disease caused by Salmonella Enteritidis (SE) is one of the important public health and economic concerns. A study was conducted to determine the effect of supplementation with 2-nitroethanol (NE) and 2-nitropropanol (NP) on Salmonella recovery of internal organs as well as on the immune gene expression in the ileum of laying hens. Thirty-six White Leghorns were orally gavaged with nalidixic acid resistant Salmonella Enteritidis (SENR). Hens were housed individually in wire-laying cages and randomly assigned to six dietary treatments: T1 = SENR unchallenged (negative control), T2 = SENR challenged (positive control), T3 = SENR challenged + 100 ppm NE, T4 = SENR challenged + 200 ppm NE, T5 = SENR challenged + 100 ppm NP, and T6 = SENR challenged + 200 ppm NP...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053814/effects-of-canola-meal-source-on-the-standardized-ileal-digestible-amino-acids-and-apparent-metabolizable-energy-contents-for-broiler-chickens
#8
D I Adewole, A Rogiewicz, B Dyck, B A Slominski
Two studies were conducted to determine the effect of canola meal source on the standardized ileal digestible amino acids (AA) and nitrogen-corrected apparent metabolizable energy (AMEn) contents of canola meal (CM) from 6 processing plants in Canada. Two plants provided CM in both mash and pellet forms, while the other 4 provided CM in mash form only, giving a total of 8 samples. In the standardized ileal digestibility (SID) assay, 280 birds were housed 7 per cage and fed a commercial starter diet from 1 to 14 d of age followed by the test diets from 15 to 21 d...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053429/house-finch-haemorhous-mexicanus-associated-mycoplasma-gallisepticum-identified-in-lesser-goldfinch-spinus-psaltria-and-western-scrub-jay-aphelocoma-californica-using-strain-specific-quantitative-pcr
#9
Catherine R Allen, Arlind Mara, Edan R Tulman, David H Ley, Steven J Geary
In 1994 Mycoplasma gallisepticum was found to be the etiologic agent of House Finch (Haemorhous mexicanus) conjunctivitis, a rapidly expanding epidemic caused by a genetically discrete, House Finch-associated strain of M. gallisepticum (HFMG). While most prominent in House Finches, HFMG has been reported in other members of the family Fringillidae, including American Goldfinches (Spinus tristis), Purple Finches (Haemorhous purpureus), Pine Grosbeaks (Pinicola enucleator), and Evening Grosbeaks (Coccothraustes vespertinus)...
October 20, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/29053015/susceptibility-biomarker-detection-in-urine-exfoliate-dna
#10
Pieranna Chiarella, Damiano Carbonari, Pasquale Capone, Delia Cavallo, Sergio Iavicoli, Antonella Mansi, Renata Sisto, Giovanna Tranfo
AIM: The occupational biomonitoring of exposures to carcinogens is carried out by measuring dose (metabolites) and susceptibility biomarkers (gene polymorphisms) in two biological matrices: urine for metabolite detection and blood for genotyping. Blood is the most common substrate but has some disadvantages including: invasiveness of the harvesting technique; need of specialized staff and equipment; and high infection risk. METHODS & RESULTS: We propose our in-house approach using urine as single sample in 20 volunteers for simultaneous detection of dose and susceptibility biomarkers in order to verify efficacy and feasibility...
October 20, 2017: Biomarkers in Medicine
https://www.readbyqxmd.com/read/29052342/supported-housing-for-adults-with-psychiatric-disabilities-how%C3%A2-tenants-confront-the-problem-of-loneliness
#11
Myra Piat, Judith Sabetti, Deborah Padgett
The objective of this study was to examine the experience of loneliness among people with psychiatric disabilities after moving from custodial housing, including group homes, boarding homes, and family-type residences to independent, supported apartments in the community. Qualitative research methods guided by a naturalistic/constructivist framework were used. Data collection occurred between May 2014 and July 2015 and consisted of individual semi-structured interviews with 24 tenants residing in five supported housing sites across three Canadian provinces; interviews were also conducted with a designated family member for each tenant and with their service providers...
October 19, 2017: Health & Social Care in the Community
https://www.readbyqxmd.com/read/29051302/diary-of-a-parliamentary-intern
#12
(no author information available yet)
Two years ago I started my internship at the House of Lords. It was a leap of faith that took me far away from the familiar world of small animal clinical practice. Now, as I sit to write my final diary piece, I wonder: was it all worth it?
October 21, 2017: Veterinary Record
https://www.readbyqxmd.com/read/29050978/chrysin-attenuates-atopic-dermatitis-by-suppressing-inflammation-of-keratinocytes
#13
Jin Kyeong Choi, Yong Hyun Jang, Soyoung Lee, Sang-Rae Lee, Young-Ae Choi, Meiling Jin, Jung Ho Choi, Jee Hun Park, Pil-Hoon Park, Hyukjae Choi, Taeg Kyu Kwon, Dongwoo Khang, Sang-Hyun Kim
We previously reported the inhibitory effect of chrysin, a natural flavonoid plentifully contained in propolis, vegetables and fruits, on the mast cell-mediated allergic reaction. In this study, we evaluated the effect of chrysin on atopic dermatitis (AD) and defined underlying mechanisms of action. We used an AD model in BALB/c mice by the repeated local exposure of 2,4-dinitrochlorobenzene (DNCB) and house dust mite (Dermatophagoides farinae extract, DFE) to the ears. Repeated alternative treatment of DNCB/DFE caused AD-like skin lesions...
October 16, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29050950/modeling-and-comparative-study-of-the-spread-of-zoonotic-visceral-leishmaniasis-from-northern-to-central-tunisia
#14
Belhassen Kaab, Elyes Zhioua
During the last twenty years, zoonotic visceral leishmaniasis (ZVL) spread from the north characterized by humid, sub-humid, and semi-arid bio-climates to the arid areas located in Central Tunisia. In order to understand the eco-epidemiological factors involved in the distribution of ZVL, both a sero-epidemiological and an entomological investigation were performed in two governorates situated in two different bio-geographical areas: Bizerte in the north and Kairouan in the center. A mathematical model for transmission of ZVL was built, describing the disease dynamic in these areas...
October 16, 2017: Acta Tropica
https://www.readbyqxmd.com/read/29050902/housing-conditions-modulate-the-reinforcing-properties-of-cocaine-in-adolescent-mice-that-binge-on-fat
#15
M Carmen Blanco-Gandía, Sandra Montagud-Romero, María A Aguilar, José Miñarro, Marta Rodríguez-Arias
Binge eating is a specific form of overeating characterized by intermittent, excessive eating. To date, several studies have addressed the effects that bingeing on fat has on the rewarding effects of drugs of abuse, but they have found contradictory and highly variable results. Housing conditions could modulate these results, as most studies employ isolated animals to measure the exact amount of food that is ingested. The aim of this study was to evaluate the effects of housing conditions on the response of mice to cocaine, modulated by bingeing on a high-fat diet during adolescence...
October 16, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/29050826/live-cell-time-lapse-imaging-and-single-cell-tracking-of-in-vitro-cultured-neural-stem-cells-tools-for-analyzing-dynamics-of-cell-cycle-migration-and-lineage-selection
#16
Katja M Piltti, Brian J Cummings, Krystal Carta, Ayla Manughian-Peter, Colleen Worne, Kulbir Singh, Danier Ong, Yuriy Maksymyuk, Michelle Khine, Aileen J Anderson
Neural stem cell (NSC) cultures have been considered technically challenging for time-lapse analysis due to high motility, photosensitivity, and growth at confluent densities. We have tested feasibility of long-term live-cell time-lapse analysis for NSC migration and differentiation studies. Here, we describe a method to study the dynamics of cell cycle, migration, and lineage selection in cultured multipotent mouse or human NSCs using single-cell tracking during a long-term, 7-14 day live-cell time-lapse analysis...
October 16, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29050522/metabolism-of-megestrol-acetate-in-vitro-and-the-role-of-oxidative-metabolites
#17
Larry House, Michael Seminerio, Snezana Mirkov, Jacqueline Ramirez, Maxwell Skor, Joseph Sachleben, Masis Isikbay, Hari Singhal, Geoffrey Greene, Donald Vander Griend, Suzanne Conzen, Mark J Ratain
There is limited knowledge regarding the metabolism of megestrol acetate (MA), as it was approved by FDA in 1971, prior to the availability of modern tools for identifying specific drug-metabolizing enzymes. We determined the cytochrome P450s (P450s) and UDP-glucuronosyltransferases (UGTs) that metabolize MA, identified oxidative metabolites, and determined pharmacologic activity at the progesterone, androgen and glucocorticoid receptors (PR, AR, and GR, respectively). Oxidative metabolites were produced using human liver microsomes (HLMs), and isolated for mass spectral (MS) and nuclear magnetic resonance (NMR) analyses...
October 20, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/29050436/sound-analysis-to-model-weight-of-broiler-chickens
#18
Ilaria Fontana, Emanuela Tullo, Lenn Carpentier, Dries Berckmans, Andy Butterworth, Erik Vranken, Tomas Norton, Daniel Berckmans, Marcella Guarino
The pattern of body weight gain during the commercial growing of broiler chickens is important to understand growth and feed conversion ratio of each flock.The application of sound analysis techniques has been widely studied to measure and analyze the amplitude and frequency of animal sounds. Previous studies have shown a significant correlation (P ≤ 0.001) between the frequency of vocalization and the age and weight of broilers. Therefore, the aim of this study was to identify and validate a model that describes the growth rate of broiler chickens based on the peak frequency of their vocalizations and to explore the possibility to develop a tool capable of automatically detecting the growth of the chickens based on the frequency of their vocalizations during the production cycle...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29050417/influence-of-swimming-time-in-alleviating-the-deleterious-effects-of-hot-summer-on-growing-muscovy-duck-performance
#19
Mohamed F A Farghly, Khalid M Mahrose, Zafar Ullah, Zaib Ur Rehman, Chan Ding
This experiment was conducted to observe the effects of varying swimming times (ST) of Muscovy ducks, raised in an open-sided house, in alleviating the deleterious effects of high temperature in hotter times of the day in the summer season on growth performance (body weight, average daily gain, feed consumption, and feed conversion ratio), carcass characteristics, body temperature, and some health aspects. We hypothesized that swimming times during the hottest periods of the day would show different performances...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29050413/laying-hens-in-aviaries-with-different-litter-substrates-behavior-across-the-flock-cycle-and-feather-lipid-content
#20
D L M Campbell, A B A Ali, D M Karcher, J M Siegford
The tiered aviary for laying hens includes a floor litter area to promote foraging and dust bathing. Data are needed on hens' use of different litter substrates and effectiveness of substrates in removing excess feather lipids to ensure a suitable litter area. Bovans White hens were housed in commercial-style aviaries with access to one of 3 litter substrates (wood shavings, straw, or plastic turf mats-AstroTurf®, n = 4 aviary pens per substrate, 144 cage-reared hens populated per pen). Litter areas were videoed across 2 d each at 4 ages: immediately following first aviary opening (25 wk), then at 28, 50, and 68 weeks...
September 1, 2017: Poultry Science
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