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https://www.readbyqxmd.com/read/29228361/identification-of-clinical-isolates-of-aspergillus-including-cryptic-species-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms
#1
M Reyes Vidal-Acuña, Maite Ruiz-Pérez de Pipaón, María José Torres-Sánchez, Javier Aznar
An expanded library of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been constructed using the spectra generated from 42 clinical isolates and 11 reference strains, including 23 different species from 8 sections (16 cryptic plus 7 noncryptic species). Out of a total of 379 strains of Aspergillus isolated from clinical samples, 179 strains were selected to be identified by sequencing of beta-tubulin or calmodulin genes. Protein spectra of 53 strains, cultured in liquid medium, were used to construct an in-house reference database in the MALDI-TOF MS...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29228021/inheritance-mode-and-mechanisms-of-resistance-to-imidacloprid-in-the-house-fly-musca-domestica-diptera-muscidae-from-china
#2
Zhuo Ma, Jing Li, Yi Zhang, Chao Shan, Xiwu Gao
Imidacloprid is a neonicotinoid insecticide that is effective against house fly, Musca domestica L., which is a major pest with the ability to develop resistance to insecticides. In the present study, we investigated the inheritance mode, the cross-resistance pattern and the mechanisms of resistance to imidacloprid. A near-isogenic house fly line (N-IRS) with 78-fold resistance to imidacloprid was used to demonstrate the mode of inheritance. The overlapping confidence limits of LC50 values and the slopes of the log concentration-probit lines between the reciprocal F1 and F1' progenies suggest that imidacloprid resistance is inherited autosomally in the house fly...
2017: PloS One
https://www.readbyqxmd.com/read/29227963/the-development-of-a-specific-pathogen-free-spf-barrier-colony-of-marmosets-callithrix-jacchus-for-aging-research
#3
Corinna N Ross, Steven Austad, Kathy Brasky, Celeste J Brown, Larry J Forney, Jonathan A Gelfond, Robert Lanford, Arlan Richardson, Suzette D Tardif
A specific pathogen free (SPF) barrier colony of breeding marmosets (Callithrix jacchus) was established at the Barshop Institute for Longevity and Aging Studies. Rodent and other animal models maintained as SPF barrier colonies have demonstrated improved health and lengthened lifespans enhancing the quality and repeatability of aging research. The marmosets were screened for two viruses and several bacterial pathogens prior to establishing the new SPF colony. Twelve founding animals successfully established a breeding colony with increased reproductive success, improved health parameters, and increased median lifespan when compared to a conventionally housed, open colony...
December 7, 2017: Aging
https://www.readbyqxmd.com/read/29227883/associations-between-medical-cannabis-and-other-drug-use-among-unstably-housed-women
#4
Meredith C Meacham, Danielle E Ramo, Alex H Kral, Elise D Riley
BACKGROUND: Several studies suggest that U.S. state-level legalization of cannabis for medical purposes may be associated with reductions in opioid use; yet its relationship with stimulant use, particularly in high-risk populations like unstably housed women, has received less attention. The purpose of this study was to estimate independent associations between medical and non-medical use of cannabis and use of stimulants and opioids among unstably housed women. METHODS: Cross-sectional data were analyzed from 245 women in the SHADOW study, a community based cohort in San Francisco, CA, in which HIV+ women were oversampled (126 HIV+ and 119 HIV-)...
December 8, 2017: International Journal on Drug Policy
https://www.readbyqxmd.com/read/29227813/speed-matters-to-raise-molar-radioactivity-fast-hplc-shortens-the-quality-control-of-c-11-pet-tracers
#5
Lukas Nics, Britta Steiner, Eva-Maria Klebermass, Cecile Philippe, Markus Mitterhauser, Marcus Hacker, Wolfgang Wadsak
INTRODUCTION: The decision whether an in-house produced short-lived radiopharmaceutical can be applied in-vivo is based on (1) the fulfilment of all quality criteria; (2) the availability of enough radioactivity for subsequent imaging; and (3) a molar activity (MA) above the set limits to guarantee safe administration without competing occupancy of the non-radioactive compound; and (4) an activity concentration, which is high enough for the application in certain preclinical studies. Hence, time reduction can be of major importance to increase final product yields, MA and activity concentrations...
November 21, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/29227438/transcanal-transpromontorial-acoustic-neuroma-surgery-results-and-facial-nerve-outcomes
#6
Daniele Marchioni, Davide Soloperto, Barbara Masotto, Cristoforo Fabbris, Stefano De Rossi, Domenico Villari, Livio Presutti
BACKGROUND: Recently, the transcanal approach for the removal of acoustic neuromas has been introduced. Facial nerve (FN) preservation is one of the main challenges of this kind of surgery. OBJECTIVE: To describe our experience in the surgical treatment of acoustic neuromas, focusing on the functional results of FN preservation after a transcanal approach. METHODS: A retrospective chart review was carried out on clinical data and videos from operations on 49 patients who underwent surgery with a totally transcanal exclusive endoscopic approach for Koos stage I-II lesions, or an enlarged transcanal transpromontorial approach for Koos stage II-III tumors, between March 2012 and February 2017...
December 7, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/29227210/separate-roles-for-chromatin-and-lamins-in-nuclear-mechanics
#7
Andrew D Stephens, Edward J Banigan, John F Marko
The cell nucleus houses, protects, and arranges the genome within the cell. Therefore, nuclear mechanics and morphology are important for dictating gene regulation, and these properties are perturbed in many human diseases, such as cancers and progerias. The field of nuclear mechanics has long been dominated by studies of the nuclear lamina, the intermediate filament shell residing just beneath the nuclear membrane. However, a growing body of work shows that chromatin and chromatin-related factors within the nucleus are an essential part of the mechanical response of the cell nucleus to forces...
December 11, 2017: Nucleus
https://www.readbyqxmd.com/read/29227133/prevalence-of-intimate-partner-violence-and-associated-factors-amongst-women-attending-antenatal-care-at-outapi-clinic-namibia-a-descriptive-survey
#8
Leonard T Bikinesi, Robert Mash, Kate Joyner
BACKGROUND: Intimate partner violence (IPV) is a significant and largely hidden public health problem for all women and, during pregnancy, can have significant effects on the health of both mother and the unborn baby. Previous Namibian studies suggest rates of IPV as high as 36%, although few studies have been conducted in primary care. AIM: To determine the prevalence of IPV amongst women attending antenatal care. SETTING: Outapi primary care clinic, Namibia...
December 6, 2017: African Journal of Primary Health Care & Family Medicine
https://www.readbyqxmd.com/read/29227036/bottom-up-nanoencapsulation-from-single-cells-to-tunable-and-scalable-cellular-spheroids-for-hair-follicle-regeneration
#9
Jin Wang, Yong Miao, Yong Huang, Bojie Lin, Xiaomin Liu, Shune Xiao, Lijuan Du, Zhiqi Hu, Malcolm Xing
Cell surface engineering technology advances cell therapeutics and tissue engineering by accurate micro/nanoscale control in cell-biomaterial ensembles and cell spheroids formation. By tailoring cell surface, microgels can encapsulate cells for versatile uses. However, microgels are coated in a thick layer to house multiple cells together but not a single cell based. Besides, excessive deposition on cell surface is detrimental to cellular functions. Herein, layer-by-layer (LbL) self-assembly to encapsulate single cell using nanogel is reported, owing to its security and tunable thickness at nanoscale, and further forms cell spheroids by physical cross-linking on nanogel-coated cells for delivery...
December 11, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29226651/quantitative-distinction-of-morphological-characteristics-of-white-blood-cells-in-peripheral-blood-through-texture-analysis-using-gray-level-co-occurrence-matrix
#10
Mai Yamamoto, Keigo Kono, Maiko Kuroki, Satoru Murakami, Ruka Hayata, Kana Nanato, Minrong Shao, Keiko Miwa, Yutaka Tsutsumi, Kazunori Okada, Sanae Kaga, Nobuo Masauzi
BACKGROUND: Texture features are valuable clues for skilled technicians to differentiate peripheral blood (PB) white blood cells (WBCs). Some studies have tried to distinguish WBCs automatically by using texture analysis. However, no study so far has applied a gray level co-occurrence matrix (GLCM) to images of PB WBCs. Here, we developed a new GLCM method, called the CM-Tx method, for automatically distinguishing PB WBCs. METHODS: We used a total of 199 images of six different types of PB WBCs, taken from PB smears of 12 healthy volunteers, as objective standard images for the analysis...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226534/commentary-on-urbanoski-et-al-2018-housing-first-and-addiction-exploring-the-evidence
#11
Nicholas Pleace
No abstract text is available yet for this article.
January 2018: Addiction
https://www.readbyqxmd.com/read/29226228/seminar-program-for-postgraduate-specialty-training-in-general-practice-proposal-for-a-5-year-thematic-catalogue
#12
Susanne Sommer, Erika Baum, Julia Magez, Jean-Francois Chenot, Gesine Weckmann, Jost Steinhäuser, Susanne Heim, Dagmar Schneider, Stephan Fuchs
Introduction: In different German regions, seminar programs have been conducted for General practice residents. In each region, selection and teaching of learning content is conducted in a different manner. So far, no structured, standardized curriculum has been implemented nationwide. We have investigated, if the development of a common 5-year program of learning topics is conceivable between the different university departments of General practice in Germany. Method: The seminar program working group of the DEGAM (German College of General Practitioners and Family Physicians) has conducted an online survey based on information gathered via preliminary telephone conference (n=7; physicians with postgraduate teaching experience) among all German university departments of General Practice and two non-university teaching institutions, identified via the internet...
2017: GMS Journal for Medical Education
https://www.readbyqxmd.com/read/29226096/feasibility-of-two-active-case-finding-approaches-for-detection-of-tuberculosis-in-bandung-city-indonesia
#13
S McAllister, B Wiem Lestari, B Sujatmiko, A Siregar, E D Sihaloho, D Fathania, N F Dewi, R C Koesoemadinata, P C Hill, B Alisjahbana
Setting: A community health clinic catchment area in the eastern part of Bandung City, Indonesia. Objective: To evaluate the feasibility of two different screening interventions using community health workers (CHWs) in detecting tuberculosis (TB) cases. Design: This was a feasibility study of 1) house-to-house TB symptom screening of five randomly selected 'neighbourhoods' in the catchment area, and 2) selected screening of household contacts of TB index patients and their neighbouring households. Acceptability was assessed through focus group discussions with key stakeholders...
September 21, 2017: Public Health Action
https://www.readbyqxmd.com/read/29226039/an-inner-city-emergency-medicine-rotation-does-not-improve-attitudes-toward-the-homeless-among-junior-medical-learners
#14
Aaron Sibley, Kathryn A Dong, Brian H Rowe
Introduction Learners in the emergency department (ED) frequently encounter individuals who are homeless. We sought to evaluate the effect of an inner city emergency medicine rotation at the Royal Alexandra Hospital (RAH) on the attitudes of medical students and residents towards this population. Methods Data were collected both pre- and post-rotation using an electronic survey. Data collected included demographic information and as well as scores on the Health Professionals' Attitudes Towards the Homeless Inventory (HPATHI)...
October 5, 2017: Curēus
https://www.readbyqxmd.com/read/29226006/next-generation-quality-assessing-the-physician-in-clinical-history-completeness-and-diagnostic-interpretations-using-funnel-plots-and-normalized-deviations-plots-in-3-854-prostate-biopsies
#15
Michael Bonert, Ihab El-Shinnawy, Michael Carvalho, Phillip Williams, Samih Salama, Damu Tang, Anil Kapoor
Background: Observational data and funnel plots are routinely used outside of pathology to understand trends and improve performance. Objective: Extract diagnostic rate (DR) information from free text surgical pathology reports with synoptic elements and assess whether inter-rater variation and clinical history completeness information useful for continuous quality improvement (CQI) can be obtained. Methods: All in-house prostate biopsies in a 6-year period at two large teaching hospitals were extracted and then diagnostically categorized using string matching, fuzzy string matching, and hierarchical pruning...
2017: Journal of Pathology Informatics
https://www.readbyqxmd.com/read/29225511/the-crystalline-sponge-method-a-solvent-based-strategy-to-facilitate-noncovalent-ordered-trapping-of-solid-and-liquid-organic-compounds
#16
Timothy R Ramadhar, Shao-Liang Zheng, Yu-Sheng Chen, Jon Clardy
A strategy that leverages solvent effects to noncovalently trap solid and unstable liquid organic compounds within a crystalline sponge ({[(ZnI2)3(tris(4-pyridyl)-1,3,5-triazine)2]·x(CHCl3)}n) in a simple, mild, and efficient fashion for target molecule structure determination via X-ray diffraction is disclosed. Host-guest structures were obtained using third-generation synchrotron radiation, and new beamline hardware allowed rapid data collection in ~5-24 minutes. This is 40-90% faster than previously reported crystalline sponge synchrotron datasets collected by us, and approximately a 150-720-fold decrease in time versus using a typical in-house diffractometer, effectively enabling the potential for high-throughput analysis...
August 21, 2017: CrystEngComm
https://www.readbyqxmd.com/read/29225237/a-biotin-streptavidin-enhanced-carbon-nanotube-amplification-strategy-for-an-ultrasensitive-immunodetection-of-polybrominated-diphenyl-ethers
#17
Xiaohan Zhang, Huisheng Zhuang
The extensive use of polybrominated diphenyl ethers (PBDEs) has resulted in its increasingly widespread presence. Especially the lower halogenated PBDEs accumulate to a greater degree than the higher halogenated PBDEs in house dust, sewage sludge, pets, or even humans. In the present work, we developed an ultrasensitive biotin-streptavidin-enhanced carbon nanotube amplification strategy for the immunodetection of PBDEs, in which single-walled carbon nanotubes were used to immobilize numerous streptavidin. Meanwhile, we used biotin conjugated horseradish peroxidase (B-HRP) and biotin conjugated Goat anti-rabbit (B-IgG) to link the HRP and IgG to CNTs by using a biotin-streptavidin system...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29225027/evolution-of-the-sauropterygian-labyrinth-with-increasingly-pelagic-lifestyles
#18
James M Neenan, Tobias Reich, Serjoscha W Evers, Patrick S Druckenmiller, Dennis F A E Voeten, Jonah N Choiniere, Paul M Barrett, Stephanie E Pierce, Roger B J Benson
Sauropterygia, a successful clade of marine reptiles abundant in aquatic ecosystems of the Mesozoic, inhabited nearshore to pelagic habitats over >180 million years of evolutionary history [1]. Aquatic vertebrates experience strong buoyancy forces that allow movement in a three-dimensional environment, resulting in structural convergences such as flippers and fish-like bauplans [2, 3], as well as convergences in the sensory systems. We used computed tomographic scans of 19 sauropterygian species to determine how the transition to pelagic lifestyles influenced the evolution of the endosseous labyrinth, which houses the vestibular sensory organ of balance and orientation [4]...
December 4, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29224877/effects-of-early-postpartum-sodium-salicylate-treatment-on-long-term-milk-intake-and-blood-parameters-of-dairy-cows
#19
A J Carpenter, C M Ylioja, L K Mamedova, K E Olagaray, B J Bradford
Previous research has shown that cows who receive treatment with nonsteroidal anti-inflammatory drugs after calving may have increased milk yield beginning near peak lactation, resulting in greater 305-d milk production. It has not been demonstrated whether this response is associated with greater feed intake following the first 3 wk of lactation. Dry matter intake (DMI) and milk yield were measured daily for 56 cows over the first 120 d in milk. Cows in their second parity and greater were blocked by parity and alternately enrolled 12 to 36 h after calving into 1 of 2 treatments: either 3 daily drenches of water or 3 daily drenches of a similar volume of water containing 125 g of sodium salicylate (SAL) beginning 12 to 36 h after calving...
December 7, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29224874/prepartum-application-of-internal-teat-sealant-or-intramammary-amoxicillin-on-dairy-heifers-effect-on-udder-health-survival-and-performance
#20
V S Machado, R C Bicalho
Intramammary infusion of antimicrobials (IA) and application of an internal teat sealant (ITS) at the end of the lactation is a common strategy to prevent and treat intramammary infections during the dry period. In heifers, precalving IA has been reported to improve udder health and milk quality; however, the effect of ITS in heifers housed in freestall barns is still unknown. The objective of this study was to investigate the effect of prepartum application of ITS or IA on udder health of dairy heifers. A total of 886 heifers were randomly allocated into 1 of 4 treatment groups when they were 245 ± 3 d pregnant...
December 7, 2017: Journal of Dairy Science
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