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https://www.readbyqxmd.com/read/29241296/evolving-aptamers-with-unnatural-base-pairs
#1
Michiko Kimoto, Ken-Ichiro Matsunaga, Ichiro Hirao
A novel technology, genetic alphabet expansion, has rapidly advanced through the successful creation of unnatural base pairs that function as a third base pair in replication. Recently, genetic alphabet expansion has been applied to some practical areas. Among them, the application to DNA aptamer generation is a good example of the broad utility of this technology. A hydrophobic unnatural base pair, Ds-Px, which exhibits high fidelity in replication as a third base pair, has been applied to an evolutionary engineering method called SELEX (Systematic Evolution of Ligands by EXponential enrichment) to generate DNA aptamers that bind to targets...
December 14, 2017: Current Protocols in Chemical Biology
https://www.readbyqxmd.com/read/29241208/exosomes-from-mir-126-overexpressing-adscs-are-therapeutic-in-relieving-acute-myocardial-ischaemic-injury
#2
Qiancheng Luo, Dongfeng Guo, Guorong Liu, Guo Chen, Min Hang, Mingming Jin
BACKGROUND/AIMS: Recent studies have indicated that exosomes play an important role in adipose-derived stem cell (ADSC) transplant-mediated ischaemic heart disease therapy. However, the treatment effect is not obvious. The aim of this study is to investigate whether ADSC-derived exosomes enriched with microRNA (miR)-126 have a more protective effect on acute myocardial infarction (AMI). METHODS: Exosomes were characterized by transmission electron microscopy, and the exosome particles were further examined using nanoparticle tracking analyses...
December 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29241203/genome-wide-transcriptome-analysis-of-estrogen-receptor-positive-and-human-epithelial-growth-factor-receptor-2-positive-breast-cancers-by-ribonucleic-acid-sequencing
#3
Pengtao Chen, Wei Song, Liangli Liu
AIM: The aim is to identify complex pathogenesis of breast cancer subtypes and disclose the whole landscape of altered transcriptional activities in these cancers. METHODS: We downloaded raw expression data from public database, and performed transcriptome analysis of 8 estrogen receptor-positive (ER+) breast cancer tissue samples, 8 human epithelial growth factor receptor 2-positive (HER2+) breast cancer tissue samples, and 3 normal breast tissues by identification, functional annotation, and prediction of upstream regulators and cell-surface biomarkers of differentially expressed genes (DEGs)...
December 14, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/29241150/assessment-of-the-roche-linear-array-hpv-genotyping-test-within-the-valgent-framework
#4
Lan Xu, Anja Oštrbenk, Mario Poljak, Marc Arbyn
BACKGROUND: Cervical cancer screening programs are switching from cytology-based screening to high-risk (hr) HPV testing. Only clinically validated tests should be used in clinical practice. OBJECTIVES: To assess the clinical performance of the Roche Linear Array HPV genotyping test (Linear Array) within the VALGENT-3 framework. STUDY DESIGN: The VALGENT framework is designed for comprehensive comparison and clinical validation of HPV tests that have limited to extended genotyping capacity...
December 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29241130/calculation-of-thallium-s-toxicity-coefficient-in-the-evaluation-of-potential-ecological-risk-index-a-case-study
#5
Yongxia Liu, Qian Wang, Wen Zhuang, Yanli Yuan, Yani Yuan, Keqin Jiao, Mantang Wang, Qing Chen
As a common pollutive metal element, Tl is very biotoxic. The potential ecological risk index (RI) proposed by Håkanson is one of the commonest methods for evaluation of ecological risk of a metal in sedimentary environment of a water body. According to the calculation principle proposed by Håkanson, the toxicity coefficient of Tl was calculated, and determined as 10 in this paper. In addition, the environmental risk of Tl in the surface sediment of the Beijing-Hangzhou Grand Canal (Zaozhuang Section) was evaluated by RI method, enrichment factor method, etc...
December 5, 2017: Chemosphere
https://www.readbyqxmd.com/read/29241129/improving-biocathode-community-multifunctionality-by-polarity-inversion-for-simultaneous-bioelectroreduction-processes-in-domestic-wastewater
#6
Hui Yun, Bin Liang, Deyong Kong, Aijie Wang
Bioelectrochemical systems (BESs) have been tentatively applied for wastewater treatment processes, but the complex composition of wastewater could lead to difficulties in establishing functional biofilm or result in performance instability. Few studies have investigated the enrichment of biocathode with domestic wastewater (DW) and the function. A biocathode with multi-pollutant removal capabilities was enriched based on polarity inverted bioanode, which was established with DW. The biocathode function was examined using model pollutants (nitrate, nitrobenzene and Acid Orange 7) supplemented as sole or mixed electron acceptors...
December 7, 2017: Chemosphere
https://www.readbyqxmd.com/read/29241118/plhint-a-knowledge-driven-computational-approach-based-on-the-intermolecular-h-bond-interactions-at-the-protein-ligand-interface-from-docking-solutions
#7
Sivakumar Prasanth Kumar
The tendency of docking scoring functions to generate crystal close conformations of ligands bound to protein structures face limitations in not reproducing the exact crystal intermolecular contacts in dock poses. Intermolecular H bond contacts enumerated at the protein-docked ligand interface can be used to train scoring models and improve virtual screening performance. There is a need to incorporate additional knowledge of protein-ligand H bond contacts in extension to crystal contacts from docking solutions within the reproducibility efficiency of the docking program...
December 6, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29241112/chromium-isotope-fractionation-during-cr-vi-reduction-in-a-methane-based-hollow-fiber-membrane-biofilm-reactor
#8
Yong-Ze Lu, Guo-Jun Chen, Ya-Nan Bai, Liang Fu, Li-Ping Qin, Raymond Jianxiong Zeng
Chromium (Cr) isotope fractionation analysis is a promising tool for monitoring Cr(VI) reduction in natural aqueous systems. In addition, large amounts of CH4 in natural aqueous sediments are oxidized to CO2 through methanotrophs, thereby mitigating emissions to the atmosphere. However, the investigations on the Cr(VI) reduction process with methanotrophs, and the associated Cr isotope fractionation patterns are scarce. In this study, we have shown that Cr(VI) reduction can occur in the presence of CH4 as the sole electron donor in a hollow-fiber membrane reactor (HfMBR) after direct bacteria enrichment from sediment samples...
December 7, 2017: Water Research
https://www.readbyqxmd.com/read/29240970/rumen-bacteria-at-work-bioaugmentation-strategies-to-enhance-biogas-production-from-cow-manure
#9
E Gozde Ozbayram, Çağrı Akyol, Bahar Ince, Canan Karakoç, Orhan Ince
AIMS: To investigate the effects of different bioaugmentation strategies for enhancing the biogas production from cow manure and evaluate microbial community patterns. METHODS AND RESULTS: Co-inoculation with cow rumen fluid and cow rumen-derived enriched microbial consortia were evaluated in anaerobic batch tests at 36 °C and 41 °C. Singular addition of both rumen fluid and enriched bioaugmentation culture had a promising enhancement on methane yields; however, the highest methane yield (311 mL CH4 / g VS at 41 °C) was achieved when the anaerobic seed sludge was co-inoculated together with rumen fluid and enriched bioaugmentation culture...
December 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29240818/genetic-dissection-of-main-and-epistatic-effects-of-qtl-based-on-augmented-triple-test-cross-design
#10
Xueli Zhang, Congwei Sun, Zheng Zhang, Zhijun Dai, Yuan Chen, Xiong Yuan, Zheming Yuan, Wenbang Tang, Lanzhi Li, Zhongli Hu
The use of heterosis has considerably increased the productivity of many crops; however, the biological mechanism underpinning the technique remains elusive. The North Carolina design III (NCIII) and the triple test cross (TTC) are powerful and popular genetic mating design that can be used to decipher the genetic basis of heterosis. However, when using the NCIII design with the present quantitative trait locus (QTL) mapping method, if epistasis exists, the estimated additive or dominant effects are confounded with epistatic effects...
2017: PloS One
https://www.readbyqxmd.com/read/29240755/integrating-transcriptomics-and-metabolomics-to-characterize-the-regulation-of-epa-biosynthesis-in-response-to-cold-stress-in-seaweed-bangia-fuscopurpurea
#11
Min Cao, Dongmei Wang, Yunxiang Mao, Fanna Kong, Guiqi Bi, Qikun Xing, Zhen Weng
Bangia fuscopurpurea is a traditional mariculture crop having high nutritional value, eicosapntemacnioc acid (EPA) production, and protein content. As an intertidal species, it can tolerate drastic changes in abiotic factors such as temperature, hydration, and light radiation; however, genomic information on the evolutionary aspect and mechanism of EPA enrichment in B. fuscopurpurea and the role of EPA in cold tolerance in this species remain elusive. We conducted transcriptome profile analysis in B. fuscopurpurea to investigate the biological functions of genes associated with resistance to various environment factors...
2017: PloS One
https://www.readbyqxmd.com/read/29240723/identi%C3%AF-cation-of-key-genes-and-pathways-in-tongue-squamous-cell-carcinoma-using-bioinformatics-analysis
#12
Huayong Zhang, Jianmin Liu, Xiaoyan Fu, Ankui Yang
BACKGROUND Tongue squamous cell carcinoma (TSCC) is a major type of oral cancers and has remained an intractable cancer over the past decades. The aim of this study was to identify differentially expressed genes (DEGs) during TSCC and reveal their potential mechanisms. MATERIAL AND METHODS The gene expression profiles of GSE13601 were downloaded from the GEO database. The GSE13601 dataset contains 57 samples, including 31 tongue SCC samples and 26 matched normal mucosa samples. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses were performed; Cytoscape software was used for the protein-protein interaction (PPI) network and module analysis of the DEGs...
December 14, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29240696/metalaxyl-degradation-by-mucorales-strains-gongronella-sp-and-rhizopus-oryzae
#13
Maria Rosário Martins, Cledir Santos, Pablo Pereira, Júlio Cruz-Morais, Nelson Lima
In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments...
December 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29240559/fluorescence-detection-enumeration-and-characterization-of-single-circulating-cells-in-vivo-technology-applications-and-future-prospects
#14
Carolin Hartmann, Roshani Patil, Charles P Lin, Mark Niedre
There are many diseases and biological processes that involve circulating cells in the bloodstream, such as cancer metastasis, immunology, reproductive medicine, and stem cell therapies. This has driven significant interest in new technologies for the study of circulating cells in small animal research models and clinically. Most currently used methods require drawing and enriching blood samples from the body, but these suffer from a number of limitations. In contrast, 'in vivo flow cytometry' (IVFC) refers to set of technologies that allow study of cells directly in the bloodstream of the organism in vivo...
December 14, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29240531/enhancing-dignity-for-older-inpatients-the-photograph-next-to-the-bed-study
#15
Ruth E Hubbard, Melissa Bak, Jacqueline Watts, David Shum, Angelica Lynch, Nancye M Peel
OBJECTIVES: Older inpatients compromised by illness and cognitive decline may be stripped of physical and cultural identifiers, making them vulnerable to erosion of dignity. This study explored the experiences of patients, carers and clinical staff in response to a simple intervention that could enhance the dignity of care for older inpatients. METHODS: All patients in a rehabilitation ward were encouraged to have a photograph of themselves next to their bed. Of those who participated, focus groups were recruited for patients, family members and staff to explore their reflections...
November 16, 2017: Clinical Gerontologist
https://www.readbyqxmd.com/read/29240511/meeting-the-nutritional-needs-of-premature-babies-their-future-is-in-our-hands
#16
Arieh Riskin
The goals of early nutrition in preterm infants are to provide all the necessary vital nutrients, achieve extra-uterine growth rates similar to fetuses of the same gestational age, and support functional neurodevelopmental outcomes that are comparable to those of infants born at term. It is vital to provide nutrition that will maximally support brain growth and development, but over-feeding with accelerated growth, fat accumulation and long-term metabolic consequences should also be avoided. Because the morbidity associated with prematurity increases nutritional and energetic demands, the basic approach is of providing early intensive nutrition...
December 2, 2017: British Journal of Hospital Medicine
https://www.readbyqxmd.com/read/29240458/genome-wide-analysis-of-uveal-melanoma-metastasis-associated-lncrnas-and-their-functional-network
#17
Binbin Xu, Ruiqi Ma, Hui Ren, Jiang Qian
Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. Up to 50% of primary UM cases will develop distant metastasis, but no effective therapies are currently available. The present study aimed to characterize the expression profile of the long noncoding RNAs (lncRNAs) and screen the potential metastasis-associated lncRNAs in UM. A genome-wide analysis of the transcriptome was performed on 11 primary UM tissues (6 metastasized and 5 nonmetastasized) through RNA sequencing. A total of 40,878 lncRNAs were detected in UM, 4,983 of which were novel candidates...
December 14, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/29240442/optical-antenna-based-fluorescence-correlation-spectroscopy-to-probe-the-nanoscale-dynamics-of-biological-membranes
#18
Pamina Martina Winkler, Raju Regmi, Valentin Flauraud, Juergen Brugger, Herve Rigneault, Jerome Wenger, Maria F Garcia-Parajo
The plasma membrane of living cells is compartmentalized at multiple spatial scales ranging from the nano- to the meso-scale. This non-random organization is crucial for a large number of cellular functions. At the nanoscale, cell membranes organize into dynamic nano-assemblies enriched by cholesterol, sphingolipids and certain types of proteins. Investigating these nano-assemblies known as lipid rafts is of paramount interest in fundamental cell biology. However, this goal requires simultaneous nanometer spatial precision and microsecond temporal resolution which is beyond the reach of common microscopes...
December 14, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29240284/extracellular-matrix-stromal-vascular-fraction-gel-conditioned-medium-accelerates-wound-healing-in-a-murine-model
#19
Chengliang Deng, Yunfan He, Jingwei Feng, Ziqing Dong, Yao Yao, Hsiaopei Mok, Maohui Lin, Lu Feng
Conditioned medium (CM) is a new treatment modality in regenerative medicine and has shown a successful outcome in wound healing. We recently introduced extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel), an adipose-derived stem cell and adipose native extracellular matrix-enriched product for cytotherapy. This study aimed to evaluate the effect of CM from ECM/SVF-gel (Gel-CM) on wound healing compared with the conventional CM from adipose tissue (Adi-CM) and stem cell (SVF-CM). In vitro wound healing effect of three CMs on keratinocytes and fibroblasts was evaluated in terms of proliferation property, migratory property, and extracellular matrix production...
December 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29240230/lc-ms-ms-profiling-of-polyphenol-enriched-leaf-stem-and-root-extracts-of-korean-humulus-japonicus-siebold-zucc-and-determination-of-their-antioxidant-effects
#20
Jin Young Choi, Kebede Taye Desta, Soo Jung Lee, Yun-Hi Kim, Sung Chul Shin, Gon-Sup Kim, Sung Joong Lee, Jae-Han Shim, Ahmet Hacımüftüo, A M Abd El-Aty
Polyphenols from ethyl acetate extracts from the leaves, stems, and roots of Korean Humulus japonicus were comprehensively profiled using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). A total of 36 polyphenols were detected, of which 26 were structurally characterized based on their [M-H]- peak, MS/MS fragmentation pattern, UV-Vis absorption, and published data. Validation data provided satisfactory results for the evaluated parameters. The determination coefficients (R2 ) exceeded or equal to 0...
December 14, 2017: Biomedical Chromatography: BMC
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