keyword
https://read.qxmd.com/read/38534964/bioprinting-of-a-liposomal-oxygen-releasing-scaffold-for-ovary-tissue-engineering
#1
JOURNAL ARTICLE
Arezoo Dadashzadeh, Saeid Moghassemi, Christiani Amorim
This study addresses a critical challenge in bioprinting for regenerative medicine, specifically the issue of hypoxia compromising cell viability in engineered tissues. To overcome this hurdle, a novel approach utilizing a microfluidic bioprinter is employed to create a two-layer structure resembling the human ovary. This structure incorporates a liposomal oxygen-releasing system to enhance cell viability. The bioprinting technique enables the simultaneous extrusion of two distinct bioinks, namely bioink A (comprising alginate 1% and 5mg/ml PEGylated fibrinogen in a 20:1 molar ratio) and bioink B (containing alginate 0...
March 27, 2024: Tissue Engineering. Part A
https://read.qxmd.com/read/38534622/development-of-ph-responsive-thermosensitive-antibacterial-and-anticancer-cs-pva-graphene-blended-hydrogels-for-controlled-drug-delivery
#2
JOURNAL ARTICLE
Saira Mansha, Amna Sajjad, Aneeqa Zarbab, Tahmina Afzal, Zakia Kanwal, Muhammad Javaid Iqbal, Mohsin Ali Raza, Sharafat Ali
Drug delivery techniques based on polymers have been investigated for their potential to improve drug solubility, reduce systemic side effects, and controlled and targeted administration at infection site. In this study, we developed a co-polymeric hydrogel composed of graphene sheets (GNS), polyvinyl alcohol (PVA), and chitosan (CS) that is loaded with methotrexate (MTX) for in vitro liver cancer treatment. Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM) was employed to check the structural properties and surface morphology...
March 18, 2024: Gels
https://read.qxmd.com/read/38530758/adrenergic-effect-on-women-s-blood-neutrophil-oxidative-activity-the-first-day-after-delivery
#3
JOURNAL ARTICLE
Oksana Olegovna Zaitseva, Victor Ivanovich Tsirkin, Inna Gennadievna Paturova, Tatiana Vitalievna Polezhaeva, Olga Nurzadinovna Solomina, Andrey Nikolaevich Khudyakov, Marta Igorevna Sergushkina, Svetlana Leonidovna Dmitrieva
OBJECTIVE: Recent studies have described a significant role for neutrophils in reproductive processes and their participation in the preparation of the cervix for childbirth and the activation of labor, in the postpartum involution of the uterus, and in the occurrence of preeclampsia. This study aimed to assess the formation of free radicals by neutrophils in the blood of women on the first day after childbirth and to characterize the adrenergic effect on this process. METHODS: Venous blood samples from 100 female volunteers aged 26-32 years who had 2 or 3 full-term deliveries were collected and analyzed...
March 26, 2024: JBRA Assisted Reproduction
https://read.qxmd.com/read/38529543/antimicrobial-effects-of-silver-nanoparticle-microspots-on-the-mechanical-properties-of-single-bacteria
#4
JOURNAL ARTICLE
Giada Caniglia, Dimitrios Valavanis, Gözde Tezcan, Joshua Magiera, Holger Barth, Joachim Bansmann, Christine Kranz, Patrick R Unwin
Silver nanoparticles (AgNPs) conjugated with polymers are well-known for their powerful and effective antimicrobial properties. In particular, the incorporation of AgNPs in biocompatible catecholamine-based polymers, such as polydopamine (PDA), has recently shown promising antimicrobial activity, due to the synergistic effects of the AgNPs, silver(I) ions released and PDA. In this study, we generated AgNPs-PDA-patterned surfaces by localised electrochemical depositions, using a double potentiostatic method via scanning electrochemical cell microscopy (SECCM)...
March 26, 2024: Analyst
https://read.qxmd.com/read/38527621/electrodiagnostic-severity-does-not-predict-short-to-mid-term-outcomes-of-cubital-tunnel-release-surgery
#5
JOURNAL ARTICLE
Nicholas Pohl, Parker Brush, Meghan Foos, Samuel Alfonsi, Pedro Beredjiklian, Daniel Fletcher
HYPOTHESIS: This study aimed to explore the prognostic value of electrodiagnostic studies (EDS) to clarify their utility in clinical practice prior to cubital tunnel release surgery as well as identify patient factors associated with patient-reported functional improvement after surgery. Our hypothesis is that patients with severe preoperative findings on EDS will tend to experience less functional improvement after surgery given the extent of ulnar nerve compressive injury. METHODS: Patients with cubital tunnel syndrome and preoperative electrodiagnostic data treated from 2012-2022 with cubital tunnel release were assessed regarding demographic information, preoperative physical exam findings, EDS findings, postoperative complications, and patient-reported outcomes...
March 23, 2024: Journal of Shoulder and Elbow Surgery
https://read.qxmd.com/read/38527566/development-of-a-hydrogel-containing-bisabolol-loaded-nanocapsules-for-the-treatment-of-atopic-dermatitis-in-a-balb-c-mice-model
#6
JOURNAL ARTICLE
Homa Karami, Mohammad Reza Niavand, Rasool Haddadi, Alireza Noriyan, Seyed Yaser Vafaei
α-Bisabolol (αBIS), a plant-derived compound with anti-inflammatory properties, is potentially a therapeutic agent for Atopic dermatitis. However, its poor water solubility and photoinstability limit its topical application. Therefore, the present study, aimed to develop cationic polymeric nanocapsules of αBIS to improve its skin delivery, photostability, and therapeutic efficacy. The αBIS- loaded nanocapsules were prepared using the solvent displacement technique. A Box-Behnken (BB) design was employed to statistically optimize formulation variables and αBIS- loaded nanocapsules characterized by particle size, surface charge and encapsulation efficiency...
March 23, 2024: International Journal of Pharmaceutics
https://read.qxmd.com/read/38527309/atp-independent-turnover-of-dinitrogen-intermediates-captured-on-the-nitrogenase-cofactor
#7
JOURNAL ARTICLE
Chi Chung Lee, Martin Stang, Markus Walter Ribbe, Yilin Hu
Nitrogenase reduces N2 to NH3 at its active-site cofactor. Previous studies of an N2-bound Mo-nitrogenase from Azotobacter vinelandii suggest binding of three N2 species via asymmetric belt-sulfur displacements in the two cofactors of its catalytic component (designated Av1*), leading to the proposal of stepwise N2 reduction involving all cofactor belt-sulfur sites; yet, the evidence for the existence of multiple N2 species on Av1* remains elusive. Here we report a study of ATP-independent, EuII/SO32- driven turnover of Av1* using GC-MS and frequency-selective pulse NMR techniques...
March 25, 2024: Angewandte Chemie
https://read.qxmd.com/read/38524583/improved-anti-cancer-effects-of-luteolin-zif-8-in-cervical-and-prostate-cancer-cell-lines
#8
JOURNAL ARTICLE
Linlin Ding, Hao Chen, Guoli Bi, Wenqi Wang, Rui Li
Luteolin, a naturally occurring pharmaceutical compound with significant antitumor properties, faces challenges in clinical applications due to its low solubility in water and limited bioavailability. To address these issues, a one-step synthesis method was employed to encapsulate luteolin within ZIF-8. The successful preparation of luteolin@ ZIF-8 nanoparticles was confirmed through various analytical techniques, including fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), laser size distribution analysis, X-ray diffraction (XRD), and release curve assessment...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38523412/a-novel-pathological-mutant-reveals-the-role-of-torsional-flexibility-in-the-serpin-breach-in-adoption-of-an-aggregation-prone-intermediate
#9
JOURNAL ARTICLE
Kamila Kamuda, Riccardo Ronzoni, Avik Majumdar, Fiona H X Guan, James A Irving, David A Lomas
Mutants of alpha-1-antitrypsin cause the protein to self-associate and form ordered aggregates ('polymers') that are retained within hepatocytes, resulting in a predisposition to the development of liver disease. The associated reduction in secretion, and for some mutants, impairment of function, leads to a failure to protect lung tissue against proteases released during the inflammatory response and an increased risk of emphysema. We report here a novel deficiency mutation (Gly192Cys), that we name the Sydney variant, identified in a patient in heterozygosity with the Z allele (Glu342Lys)...
March 24, 2024: FEBS Journal
https://read.qxmd.com/read/38522233/electrochemical-biosensing-platform-based-on-aunws-rgo-cmc-pedot-pss-composite-for-the-detection-of-superoxide-anion-released-from-living-cells
#10
JOURNAL ARTICLE
Renjun Jiang, Jiaojiao Liu, Xiaoqiang Liu, Jadranka Travas Sejdic
Detection of superoxide anion (O2 ·- ) levels holds significant importance for the diagnosis and even clinical treatments of oxidative stress-related diseases. Herein, we prepared a composite electrode material to encapsulate copper-zinc superoxide dismutase (SOD1) for biosensing of O2 ·- . The sensing material consists of gold nanowires (AuNWs), reduced graphene oxide (rGO), carboxymethyl cellulose (CMC) and PEDOT:PSS. CMC provides abundant -COOH to bind SOD1, with a high adsorption coverage of 1...
March 19, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38520950/lipid-coated-nanocrystals-of-paclitaxel-as-dry-powder-for-inhalation-characterization-in-vitro-performance-and-pharmacokinetic-assessment
#11
JOURNAL ARTICLE
Manish Kumar, Abhishek Jha, Kanchan Bharti, Manjit Manjit, Pradnya Kumbhar, Vividha Dhapte-Pawar, Brahmeshwar Mishra
BACKGROUND: Nanocrystals can be produced as a dry powder for inhalation (DPIs) to deliver high doses of drug to the lungs, owing to their high payload and stability to the shear stress of aerosolization force. Furthermore, lipid-coated nanocrystals can be formulated to improve the drug accumulation and retention in lung. OBJECTIVE: The present work involved the fabrication of paclitaxel nanocrystals using hydrophilic marine biopolymer fucoidan as a stabilizer. Thereafter, fabricated nanocrystals (FPNC) were surface-modified with phospholipid to give lipid-coated nanocrystals (Lipo-NCs)...
March 21, 2024: Colloids and Surfaces. B, Biointerfaces
https://read.qxmd.com/read/38520869/targeted-inhibition-of-stat3-tyr705-by-xanthatin-alleviates-osteoarthritis-progression-through-the-nf-%C3%AE%C2%BAb-signaling-pathway
#12
JOURNAL ARTICLE
Yangjun Xu, Zhuolin Chen, Xuanyuan Lu, Jiewen Zheng, Xuewen Liu, Tan Zhang, Wanlei Yang, Yu Qian
The transcription factor, signal transducer, and stimulator of transcription 3 (STAT3) is a potential target in osteoarthritis (OA) treatment. Although xanthatin (XA), a biologically active substance derived from Xanthium strumarium L, specifically inhibits STAT3 phosphorylation at Tyr705, the mechanism underlying its inhibitory effect on OA progression remains unclear. In this study, our objective was to explore the therapeutic effects exerted by XA on OA and the underlying molecular mechanisms. The effects of XA treatment on mouse OA models subjected to destabilization of the medial meniscus using medial collateral ligament transection, as well as on interleukin-1β (IL-1β)-induced mouse chondrocytes, were examined...
March 22, 2024: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/38519095/synaptic-electrophysiology-of-the-drosophila-neuromuscular-junction
#13
JOURNAL ARTICLE
Bing Zhang, Bryan Stewart
Chemical synaptic transmission is an important means of neuronal communication in the nervous system. Upon the arrival of an action potential, the nerve terminal experiences an influx of calcium ions, which in turn trigger the exocytosis of synaptic vesicles (SVs) and the release of neurotransmitters into the synaptic cleft. Transmitters elicit synaptic responses in the postsynaptic cell by binding to and activating specific receptors. This is followed by the recycling of SVs at presynaptic terminals. The Drosophila larval neuromuscular junction (NMJ) shares many structural and functional similarities to synapses in other animals, including humans...
March 22, 2024: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38519094/focal-recording-of-synaptic-currents-from-single-boutons-at-the-drosophila-larval-neuromuscular-junction
#14
JOURNAL ARTICLE
Bing Zhang, Bryan Stewart
Focal recording is an extracellular method for studying synaptic transmission at the Drosophila larval neuromuscular junction (NMJ) designed for the study of synaptic activity of one or a few synaptic boutons rather than the ensemble activity of all the boutons as occurs with intracellular recording or two-electrode voltage-clamp. This is a useful technique for investigating the properties of different motor neurons that innervate the same muscle, applying statistical analysis to discrete synaptic events, and investigating the heterogeneity of synaptic release properties among boutons...
March 22, 2024: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38518556/clozapine-laden-carbon-dots-delivered-to-the-brain-via-an-intranasal-pathway-synthesis-characterization-ex-vivo-and-in-vivo-studies
#15
JOURNAL ARTICLE
Hetal P Patel, Priya H Desai, Rikin V Patel, Sandesh N Lodha, Anil H Gore, Pravin O Patil, Bhargavi V Desai, Ditixa T Desai, Bhavin A Vyas, Mark D P Willcox, Furqan A Maulvi
Clozapine, which is widely used to treat schizophrenia, shows low bioavailability due to poor solubility and high first-pass metabolism. The study aimed to design clozapine-loaded carbon dots (CDs) to enhance availability of the clozapine to the brain via intranasal pathway. The CDs were synthesized by pyrolysis of citric acid and urea at 200 °C by hydrothermal technique and characterized by photoluminescence, transmission electron microscopy (TEM), X-ray Photoelectron Spectrometer (XPS), and Fourier transform infrared spectrum (FTIR)...
March 20, 2024: Colloids and Surfaces. B, Biointerfaces
https://read.qxmd.com/read/38518220/hyper-responsive-chemiluminescent-probe-reveals-distinct-pyrase-activity-in-pseudomonas-aeruginosa
#16
JOURNAL ARTICLE
Rozan Tannous, Omri Shelef, Tal Kopp, Micha Fridman, Doron Shabat
Pyrrolidone carboxyl peptidase, commonly known as PYRase, is an exopeptidase that catalytically cleaves an N -terminal pyroglutamic acid from peptides or proteins. The diverse functions of PYRases in bacterial enzymology have prompted the development of various bacterial diagnostic techniques. However, the specific physiological role and activity of this enzyme across the bacterial kingdom remain unclear. Here, we present a functional phenoxy-1,2-dioxetane chemiluminescent probe (PyrCL) that can selectively detect PYRase activity in both Gram-positive and Gram-negative bacteria...
March 22, 2024: Bioconjugate Chemistry
https://read.qxmd.com/read/38513298/a-silk-based-hydrogel-containing-dexamethasone-and-lipoic-acid-microcrystals-for-local-delivery-to-the-inner-ear
#17
JOURNAL ARTICLE
Haixia Xiong, Peili Zhang, Dongcheng Wang, Zhimin Zhou, Jianjun Sun, Mingfang Diao
Local drug delivery has been exploited recently to treat hearing loss, as this method can both bypass the blood-labyrinth barrier and provide sustained drug release. Combined drug microcrystals (MCs) offer additional advantages for sensorineural hearing loss treatment via intratympanic (IT) injection due to their shape effect and combination strategy. In this study, to endow viscous effects of hydrogels, nonspherical dexamethasone (DEX) and lipoic acid (LA) MCs were incorporated into silk fibroin (SF) hydrogels, which were subsequently administered to the tympanic cavity to investigate their pharmaceutical properties...
March 16, 2024: Colloids and Surfaces. B, Biointerfaces
https://read.qxmd.com/read/38511302/strontium-doped-hydroxyapatite-coating-improves-osteo-angiogenesis-for-ameliorative-graft-bone-integration-via-the-macrophage-derived-cytokines-mediated-integrin-signal-pathway
#18
JOURNAL ARTICLE
Tianwu Chen, Xiaopei Wu, Peng Zhang, Wei Wu, Honglian Dai, Shiyi Chen
Polyethylene terephthalate (PET) artificial ligaments, renowned for their superior mechanical properties, have been extensively adopted in anterior cruciate ligament (ACL) reconstruction surgeries. However, the inherent bio-inertness of PET introduces formidable barriers to graft-bone integration, a critical aspect of rehabilitation. Previous interventions, ranging from surface roughening to chemical modifications, have aimed to address this challenge; however, consistently effective techniques for inducing graft-bone integration remain scarce...
March 21, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38510976/decoding-phenotypic-screening-a-comparative-analysis-of-image-representations
#19
JOURNAL ARTICLE
Adriana Borowa, Dawid Rymarczyk, Marek Żyła, Maciej Kańdula, Ana Sánchez-Fernández, Krzysztof Rataj, Łukasz Struski, Jacek Tabor, Bartosz Zieliński
Biomedical imaging techniques such as high content screening (HCS) are valuable for drug discovery, but high costs limit their use to pharmaceutical companies. To address this issue, The JUMP-CP consortium released a massive open image dataset of chemical and genetic perturbations, providing a valuable resource for deep learning research. In this work, we aim to utilize the JUMP-CP dataset to develop a universal representation model for HCS data, mainly data generated using U2OS cells and CellPainting protocol, using supervised and self-supervised learning approaches...
December 2024: Computational and Structural Biotechnology Journal
https://read.qxmd.com/read/38508349/mzb1-regulates-cellular-proliferation-mitochondrial-dysfunction-and-inflammation-and-targets-the-pi3k-akt-signaling-pathway-in-acute-pancreatitis
#20
JOURNAL ARTICLE
Mengtao Xu, Yong Feng, Xuelian Xiang, Li Liu, Guodu Tang
BACKGROUND: Acute pancreatitis (AP) is a pathological condition characterized by the premature release and activation of trypsinogens and other enzyme precursors. In severe cases, the mortality rates are in the range of 20-30% and may even be as high as 50%. Though various prophylaxes are available for AP, the mechanism of its progression is unclear. Marginal zone B and B-1 cell-specific protein 1 (MZB1) is found in the endoplasmic reticulum (ER) where it is expressed exclusively in the B cells there...
March 18, 2024: Cellular Signalling
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