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https://www.readbyqxmd.com/read/28655216/phenotypic-and-genomic-survey-on-organic-acid-utilization-profile-of-pseudomonas-mendocina-strain-s5-2-a-vineyard-soil-isolate
#1
Teik Min Chong, Jian-Woon Chen, Wah-Seng See-Too, Choo-Yee Yu, Geik-Yong Ang, Yan Lue Lim, Wai-Fong Yin, Catherine Grandclément, Denis Faure, Yves Dessaux, Kok-Gan Chan
Root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes inhabiting the rhizosphere. The exudates shape the microflora associated with the plant, as well as influences the plant health and productivity. Therefore, a better understanding of the trophic link that is established between the plant and the associated bacteria is necessary. In this study, a comprehensive survey on the utilization of grapevine and rootstock related organic acids were conducted on a vineyard soil isolate which is Pseudomonas mendocina strain S5...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28654691/whole-gene-analysis-of-two-groups-of-hepatitis-b-virus-c-d-inter-genotype-recombinant-strains-isolated-in-tibet-china
#2
Tiezhu Liu, Fuzhen Wang, Shuang Zhang, Feng Wang, Qingling Meng, Guomin Zhang, Fuqiang Cui, Dorji Dunzhu, Wenjiao Yin, Shengli Bi, Liping Shen
Tibet is a highly hepatitis B virus (HBV) endemic area. Two types of C/D recombinant HBV are commonly isolated in Tibet and have been previously described. In an effort to better understand the molecular characteristic of these C/D recombinant strains from Tibet, we undertook a multistage random sampling project to collect HBsAg positive samples. Molecular epidemiological and bio-informational technologies were used to analyze the characteristics of the sequences found in this study. There were 60 samples enrolled in the survey, and we obtained 19 whole-genome sequences...
2017: PloS One
https://www.readbyqxmd.com/read/28654687/combined-single-cell-quantitation-of-host-and-siv-genes-and-proteins-ex-vivo-reveals-host-pathogen-interactions-in-individual-cells
#3
Diane L Bolton, Kathleen McGinnis, Greg Finak, Pratip Chattopadhyay, Raphael Gottardo, Mario Roederer
CD4 T cells harboring HIV-1/SIV represent a formidable hurdle to eradicating infection, and yet their detailed phenotype remains unknown. Here we integrate two single-cell technologies, flow cytometry and highly multiplexed quantitative RT-PCR, to characterize SIV-infected CD4 T cells directly ex vivo. Within individual cells, we correlate the cellular phenotype, in terms of host protein and RNA expression, with stages of the viral life cycle defined by combinatorial expression of viral RNAs. Spliced RNA+ infected cells display multiple memory and activation phenotypes, indicating virus production by diverse CD4 T cell subsets...
June 27, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28654651/herpes-simplex-virus-1-and-2-educational-assessment-of-young-adults-in-rural-southwest-virginia
#4
Shantal S Hover, Andrea S Bertke
PURPOSE: Herpes simplex virus 2 (HSV-2) causes genital herpes, one of the most common sexually transmitted infections (STIs) in the U.S. HSV-1, commonly associated with cold sores, is increasing as a cause of genital herpes. Abstinence-only sexual health classes, commonly taught in Virginia, generate young adults who are under-educated in sexual health, increasing STI risks. College students in southwest Virginia were surveyed to assess comprehensiveness of high school health education regarding HSV-1 and HSV-2 and to identify students' preferred methods for STI education...
2017: PloS One
https://www.readbyqxmd.com/read/28654584/a-novel-noncontact-diffuse-correlation-spectroscopy-device-for-assessing-blood-flow-in-mastectomy-skin-flaps-a-prospective-study-in-patients-undergoing-prosthesis-based-reconstruction
#5
Nneamaka B Agochukwu, Chong Huang, Mingjun Zhao, Ahmed A Bahrani, Li Chen, Patrick McGrath, Guoqiang Yu, Lesley Wong
A new advanced technology, noncontact diffuse correlation spectroscopy, has been recently developed for the measurement of tissue blood flow through analyzing the motions of red blood cells in deep tissues. This technology is portable, inexpensive, and noninvasive, and can measure up to 1.5-cm tissue depth. In this prospective study, the authors aimed to explore the use of this novel device in the prediction of mastectomy skin flap necrosis. The noncontact diffuse correlation spectroscopy device was used to measure mastectomy skin flap flow in patients undergoing mastectomy and immediate implant-based breast reconstruction before and immediately after mastectomy, and after placement of the prosthesis...
July 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28654194/ecogenetics-an-r-package-for-the-management-and-exploratory-analysis-of-spatial-data-in-landscape-genetics
#6
Leandro G Roser, Laura I Ferreyra, Beatriz O Saidman, Juan C Vilardi
The integration of ecology and genetics has become established in recent decades, in hand with the development of new technologies, whose implementation is allowing an improvement of the tools used for data analysis. In a landscape genetics context, integrative management of population information from different sources can make spatial studies involving phenotypic, genotypic and environmental data simpler, more accessible and faster. Tools for exploratory analysis of autocorrelation can help to uncover the spatial genetic structure of populations and generate appropriate hypotheses in searching for possible causes and consequences of their spatial processes...
June 27, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28653809/a-prenatal-diagnosis-of-mosaic-trisomy-5-reveals-a-postnatal-complete-uniparental-disomy-of-chromosome-5-with-multiple-congenital-anomalies
#7
Andrew M Reittinger, Benjamin M Helm, Debra J Boles, Inder K Gadi, Samantha A Schrier Vergano
Mosaic trisomy 5 is a very rare condition in liveborns, with few cases reported in the last four decades. There are some reports of prenatally diagnosed mosaic trisomy 5 resulting in phenotypically normal offspring, suggesting a low level of mosaicism, but there are also reports associated with multiple congenital anomalies, cardiovascular malformations, and intrauterine growth restriction. We report an infant male diagnosed with mosaic trisomy 5 (5/15 cells) via amniocentesis. The patient was subsequently found to have uniparental disomy 5 (UPD5) by postnatal chromosome microarray, but high-resolution chromosome analysis on peripheral blood did not identify trisomy 5...
June 27, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28653552/the-agency-of-patients-and-carers-in-medical-care-and-self-care-technologies-for-interacting-with-doctors
#8
Francisco Nunes, Tariq Andersen, Geraldine Fitzpatrick
People living with Parkinson's disease engage in self-care for most of the time but, two or three times a year, they meet with doctors to re-evaluate the condition and adjust treatment. Patients and (informal) carers participate actively in these encounters, but their engagement might change as new patient-centred technologies are integrated into healthcare infrastructures. Drawing on a qualitative study that used observations and interviews to investigate consultations, and digital ethnography to understand interactions in an online community, we describe how patients and carers living with Parkinson's participate in the diagnosis and treatment decisions, engage in discussions to learn about certain topics, and address inappropriate medication...
June 1, 2017: Health Informatics Journal
https://www.readbyqxmd.com/read/28653543/current-induced-magnetic-polarons-in-a-colloidal-quantum-dot-device
#9
Franziska Muckel, Charles J Barrows, Arthur Graf, Alexander Schmitz, Christian S Erickson, Daniel R Gamelin, Gerd Bacher
Electrical spin manipulation remains a central challenge for the realization of diverse spin-based information processing technologies. Motivated by the demonstration of confinement-enhanced sp-d exchange interactions in colloidal diluted magnetic semiconductor (DMS) quantum dots (QDs), such materials are considered promising candidates for future spintronic or spin-photonic applications. Despite intense research into DMS QDs, electrical control of their magnetic and magneto-optical properties remains a daunting goal...
June 27, 2017: Nano Letters
https://www.readbyqxmd.com/read/28653203/ras-screening-in-colorectal-cancer-a-comprehensive-analysis-of-the-results-from-the-uk-neqas-colorectal-cancer-external-quality-assurance-schemes-2009-2016
#10
Susan D Richman, Jennifer Fairley, Rachel Butler, Zandra C Deans
Evidence strongly indicates that extended RAS testing should be undertaken in mCRC patients, prior to prescribing anti-EGFR therapies. With more laboratories implementing testing, the requirement for External Quality Assurance schemes increases, thus ensuring high standards of molecular analysis. Data was analysed from 15 United Kingdom National External Quality Assessment Service (UK NEQAS) for Molecular Genetics Colorectal cancer external quality assurance (EQA) schemes, delivered between 2009 and 2016. Laboratories were provided annually with nine colorectal tumour samples for genotyping...
June 26, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28653118/quantitative-pneumatic-otoscopy-using-a-light-based-ranging-technique
#11
Ryan L Shelton, Ryan M Nolan, Guillermo L Monroy, Paritosh Pande, Michael A Novak, Ryan G Porter, Stephen A Boppart
Otitis media is the leading cause of hearing loss in children. It is commonly associated with fluid in the ear, which can result in up to 45 dB of hearing loss for extended periods of time during a child's most important developmental years. Accurate assessment of middle ear effusions is an important part of understanding otitis media. Current technologies used to diagnose otitis media with effusion are pneumatic otoscopy, tympanometry, and acoustic reflectometry. While all of these techniques can reasonably diagnose the presence of an effusion, they provide limited information about the infection present behind the tympanic membrane...
June 26, 2017: Journal of the Association for Research in Otolaryngology: JARO
https://www.readbyqxmd.com/read/28652976/noninvasive-real-time-characterization-of-non-melanoma-skin-cancers-with-handheld-optoacoustic-probes
#12
Amalina Binte Ebrahim Attia, Sai Yee Chuah, Daniel Razansky, Chris Jun Hui Ho, Pinky Malempati, U S Dinish, Renzhe Bi, Chit Yaw Fu, Steven J Ford, Joyce Siong-See Lee, Melissa Wee Ping Tan, Malini Olivo, Steven Tien Guan Thng
Currently, imaging technologies that enable dermsurgeons to visualize non-melanoma skin cancers (NMSC) in vivo preoperatively are lacking, resulting in excessive or incomplete removal. Multispectral optoacoustic tomography (MSOT) is a volumetric imaging tool to differentiate tissue chromophores and exogenous contrast agents, based on differences in their spectral signatures and used for high-resolution imaging of functional and molecular contrast at centimeter scale depth. We performed MSOT imaging with two- and three-dimensional handheld scanners on 21 Asian patients with NMSC...
September 2017: Photoacoustics
https://www.readbyqxmd.com/read/28652629/-observant-ii-observational-study-of-effectiveness-of-transcatheter-aortic-valve-implantation-with-new-generation-devices-for-severe-aortic-stenosis-treatment-study-protocol
#13
Fulvia Seccareccia, Giuseppe Tarantini, Francesco Bedogni, Sergio Berti, Gennaro Santoro, Corrado Tamburino, Gian Paolo Ussia, Marco Barbanti, Massimo Baiocchi, Marco Ranucci, Paola D'Errigo, Stefano Rosato, Giuseppe Musumeci
BACKGROUND: The rapid spread of transcatheter aortic valve implantation (TAVI) for the treatment of severe symptomatic aortic stenosis in the last decade in Italy has led to a gradually increasing use of TAVI procedures also in patients potentially eligible for aortic valve replacement (AVR). For this subset of patients, the OBSERVANT study (2011-2012) evaluated the short- and medium term outcome of TAVI vs AVR, at least for the first generations of TAVI devices, but failed to gather information on all the technological innovations occurred in recent years...
June 2017: Giornale Italiano di Cardiologia
https://www.readbyqxmd.com/read/28652496/harnessing-temporal-modes-for-multi-photon-quantum-information-processing-based-on-integrated-optics
#14
G Harder, V Ansari, T J Bartley, B Brecht, C Silberhorn
In the last few decades, there has been much progress on low loss waveguides, very efficient photon-number detectors and nonlinear processes. Engineered sum-frequency conversion is now at a stage where it allows operation on arbitrary temporal broadband modes, thus making the spectral degree of freedom accessible for information coding. Hereby the information is often encoded into the temporal modes of a single photon. Here, we analyse the prospect of using multi-photon states or squeezed states in different temporal modes based on integrated optics devices...
August 6, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28652495/quantum-photonic-network-and-physical-layer-security
#15
Masahide Sasaki, Hiroyuki Endo, Mikio Fujiwara, Mitsuo Kitamura, Toshiyuki Ito, Ryosuke Shimizu, Morio Toyoshima
Quantum communication and quantum cryptography are expected to enhance the transmission rate and the security (confidentiality of data transmission), respectively. We study a new scheme which can potentially bridge an intermediate region covered by these two schemes, which is referred to as quantum photonic network. The basic framework is information theoretically secure communications in a free space optical (FSO) wiretap channel, in which an eavesdropper has physically limited access to the main channel between the legitimate sender and receiver...
August 6, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28652492/noise-management-to-achieve-superiority-in-quantum-information-systems
#16
REVIEW
Kae Nemoto, Simon Devitt, William J Munro
Quantum information systems are expected to exhibit superiority compared with their classical counterparts. This superiority arises from the quantum coherences present in these quantum systems, which are obviously absent in classical ones. To exploit such quantum coherences, it is essential to control the phase information in the quantum state. The phase is analogue in nature, rather than binary. This makes quantum information technology fundamentally different from our classical digital information technology...
August 6, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28652491/quantum-communication-with-coherent-states-of-light
#17
REVIEW
Imran Khan, Dominique Elser, Thomas Dirmeier, Christoph Marquardt, Gerd Leuchs
Quantum communication offers long-term security especially, but not only, relevant to government and industrial users. It is worth noting that, for the first time in the history of cryptographic encoding, we are currently in the situation that secure communication can be based on the fundamental laws of physics (information theoretical security) rather than on algorithmic security relying on the complexity of algorithms, which is periodically endangered as standard computer technology advances. On a fundamental level, the security of quantum key distribution (QKD) relies on the non-orthogonality of the quantum states used...
August 6, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28652374/crispr-cas9-conformational-activation-as-elucidated-from-enhanced-molecular-simulations
#18
Giulia Palermo, Yinglong Miao, Ross C Walker, Martin Jinek, J Andrew McCammon
CRISPR-Cas9 has become a facile genome editing technology, yet the structural and mechanistic features underlying its function are unclear. Here, we perform extensive molecular simulations in an enhanced sampling regime, using a Gaussian-accelerated molecular dynamics (GaMD) methodology, which probes displacements over hundreds of microseconds to milliseconds, to reveal the conformational dynamics of the endonuclease Cas9 during its activation toward catalysis. We disclose the conformational transition of Cas9 from its apo form to the RNA-bound form, suggesting a mechanism for RNA recruitment in which the domain relocations cause the formation of a positively charged cavity for nucleic acid binding...
June 26, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28652298/a-patient-specific-intracranial-aneurysm-model-with-endothelial-lining-a-novel-in-vitro-approach-to-bridge-the-gap-between-biology-and-flow-dynamics
#19
Naoki Kaneko, Toshihiro Mashiko, Katsunari Namba, Satoshi Tateshima, Eiju Watanabe, Kensuke Kawai
OBJECTIVES: To develop an in vitro model for studying the biological effect of complex-flow stress on endothelial cells in three-dimensional (3D) patient-specific vascular geometry. MATERIALS AND METHODS: A vessel replica was fabricated with polydimethylsiloxanes using 3D printing technology from vascular image data acquired by rotational angiography. The vascular model was coated with fibronectin and immersed in a tube filled with a cell suspension of endothelium, and then cultured while being slowly rotated in three dimensions...
June 26, 2017: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/28652227/reducing-parental-uncertainty-around-childhood-cancer-implementation-decisions-and-design-trade-offs-in-developing-an-electronic-health-record-linked-mobile-app
#20
Keith Marsolo, William Shuman, Jeremy Nix, Caroline F Morrison, Larry L Mullins, Ahna Lh Pai
BACKGROUND: Parents of children newly diagnosed with cancer are confronted with multiple stressors that place them at risk for significant psychological distress. One strategy that has been shown to help reduce uncertainty is the provision of basic information; however, families of newly diagnosed cancer patients are often bombarded with educational material. Technology has the potential to help families manage their informational needs and move towards normalization. OBJECTIVE: The aim of this study was to create a mobile app that pulls together data from both the electronic health record (EHR) and vetted external information resources to provide tailored information to parents of newly diagnosed children as one method to reduce the uncertainty around their child's illness...
June 26, 2017: JMIR Research Protocols
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