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Yoshinobu Ohsaki, Takaaki Sasaki, Satoshi Endo, Masahiro Kitada, Shunsuke Okumura, Noriko Hirai, Yoshihiro Kazebayashi, Eri Toyoshima, Yasushi Yamamoto, Kaneyoshi Takeyama, Susumu Nakajima, Isao Sakata
BACKGROUND: We observed red autofluorescence emanating from bronchial cancer lesions using a sensitive color-fluorescence endoscopy system. We investigated to clarify the origin of the red autofluorescence. METHODS: The wavelengths of the red autofluorescence emanating from lesions were measured in eight patients using a spectrum analyzer and compared based on pathologic findings. Red autofluorescence at 617.3, 617.4, 619.0, and 617.1 nm was emitted by normal bronchus, inflamed tissue, tissue exhibiting mild dysplasia, and malignant lesions, respectively...
April 26, 2017: BMC Cancer
Deng-Pan Wu, Tian-Yu Lin, Li-Ru Bai, Jin-Lan Huang, Yan Zhou, Nan Zhou, Sheng-Lei Zhong, Shan Gao, Xiao-Xing Yin
In spite of the promising initial treatment responses presented by photodynamic therapy (PDT), 5-year recurrence rates remain high level. Therefore, improvement in the efficacy of PDT is needed. There are reports showing that connexin(Cx) 26-composed gap junctional intercellular communication (GJIC) enhances the intercellular propagation of "death signal", thereby increasing chemotherapeutic cytotoxicity. However, it is unclear whether Cx26-formed GJIC has an effect on PDT phototoxicity. The results in the present study showed that Cx26-composed GJ formation at high density enhances the phototoxicity of Photofrin-PDT...
April 18, 2017: Journal of Biophotonics
Haixia Qiu, Michele M Kim, Rozhin Penjweini, Timothy C Zhu
No abstract text is available yet for this article.
April 1, 2017: Journal of Biomedical Optics
Li Li, Huiyu Wang, Haiping Wang, Lijun Li, Pan Wang, Xiaobing Wang, Quanhong Liu
Photodynamic therapy (PDT) is a promising method for neoplastic and nonneoplastic diseases. In this study, we utilized sinoporphyrin sodium (DVDMS) as a sensitizer combined with light to investigate its cytotoxic effect on different cell lines. For this purpose, we chose bovine serum albumin (BSA) as a model to explore the mechanism of PDT-induced cell death at a molecular level. Our findings indicated that the combined treatment significantly suppressed cell survival. Fluorescence spectroscopy revealed a strong interaction between DVDMS and BSA molecules in aqueous solution, affecting DVDMS' targeting distribution and metabolism...
March 2, 2017: Scientific Reports
Liyi Huang, Grzegorz Szewczyk, Tadeusz Sarna, Michael R Hamblin
It is known that noncationic porphyrins such as Photofrin (PF) are effective in mediating antimicrobial photodynamic inactivation (aPDI) of Gram-positive bacteria or fungi. However, the aPDI activity of PF against Gram-negative bacteria is accepted to be extremely low. Here we report that the nontoxic inorganic salt potassium iodide (KI) at a concentration of 100 mM when added to microbial cells (10(8)/mL) + PF (10 μM hematoporphyrin equivalent) + 415 nm light (10 J/cm(2)) can eradicate (>6 log killing) five different Gram-negative species (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, and Acinetobacter baumannii), whereas no killing was obtained without KI...
February 23, 2017: ACS Infectious Diseases
Y Ong, R Penjweini, A Yodh, T Zhu
PURPOSE: To investigate the variation in blood flow during PDT and to determine the oxygen supplying rate, g, to refine the empirical macroscopic model. METHODS: RIF tumors were propagated in female C3H mice by injecting cell suspension at a concentration of 1×10(6) cells/ml on the shoulder region. Photosensitizer(e.g., HPPH) was injected into the mice through tail vein at a concentration of 0.25 mg/kg when the tumors grew to ∼6 mm in diameter and depth. Temporal changes of tissue oxygen concentration were recorded prior, during and post PDT treatment using OxyLite single-channel tissue pO2 monitor(OXFORD OPTRONIX)...
June 2016: Medical Physics
David Kessel
In prior studies, we have identified the ability of low-level lysosomal photodamage to potentiate the phototoxic effect of subsequent photodamage to mitochondria. The mechanism involves calpain-mediated cleavage of the autophagy-associated protein ATG5 to form a proapoptotic fragment (tATG5). In this report, we explore the permissible time lag between the two targeting procedures along with the effect of simultaneously targeting both lysosomes and mitochondria. This was found to be as effective as the sequential protocol with no gap between the irradiation steps...
March 2017: Photochemistry and Photobiology
Michele M Kim, Rozhin Penjweini, Nathan R Gemmell, Israel Veilleux, Aongus McCarthy, Gerald S Buller, Robert H Hadfield, Brian C Wilson, Timothy C Zhu
Accurate photodynamic therapy (PDT) dosimetry is critical for the use of PDT in the treatment of malignant and nonmalignant localized diseases. A singlet oxygen explicit dosimetry (SOED) model has been developed for in vivo purposes. It involves the measurement of the key components in PDT-light fluence (rate), photosensitizer concentration, and ground-state oxygen concentration ([³O₂])-to calculate the amount of reacted singlet oxygen ([¹O₂]rx), the main cytotoxic component in type II PDT. Experiments were performed in phantoms with the photosensitizer Photofrin and in solution using phosphorescence-based singlet oxygen luminescence dosimetry (SOLD) to validate the SOED model...
December 6, 2016: Cancers
Ye-Kyu Park, Choong-Hak Park
OBJECTIVE: The management of cervical intraepithelial neoplasia (CIN) and early invasive cancer of the uterine cervix is very difficult to approach, especially in case of young woman who wants to preserve her fertility. Conization of the cervix may have various kinds of disadvantage. The objective of this clinical retrospective study is to investigate the therapeutic effects and clinical efficacy of photodynamic therapy (PDT) including combined chemo-photodynamic therapy in patients with pre-malignant CIN and malignant invasive cervical cancer...
November 2016: Obstetrics & Gynecology Science
Nayan Patel, Paula Pera, Penny Joshi, Mykhaylo Dukh, Walter A Tabaczynski, Kevin E Siters, Mark Kryman, Ravindra R Cheruku, Farukh Durrani, Joseph R Missert, Ramona Watson, Tymish Y Ohulchanskyy, Erin C Tracy, Heinz Baumann, Ravindra K Pandey
We report herein the synthesis and biological efficacy of near-infrared (NIR), bacteriochlorin analogues: 3-(1'-butyloxy)ethyl-3-deacetyl-bacteriopurpurin-18-N-butylimide methyl ester (3) and the corresponding carboxylic acid 10. In in vitro assays, compared to its methyl ester analogue 3, the corresponding carboxylic acid derivative 10 showed higher photosensitizing efficacy. However, due to drastically different pharmacokinetics in vivo, the PS 3 (HPLC purity >99%) showed higher tumor uptake and long-term tumor cure than 10 (HPLC purity >96...
November 10, 2016: Journal of Medicinal Chemistry
Monica Neagu, Carolina Constantin, Mircea Tampa, Clara Matei, Andreea Lupu, Emilia Manole, Rodica-Mariana Ion, Concettina Fenga, Aristidis M Tsatsakis
Metallo-phthalocyanines due to their photophysical characteristics as high yield of triplet state and long lifetimes, appear to be good candidates for photodynamic therapy (PDT). Complexes with diamagnetic metals such as Zn2+, Al3+ Ga3+ and In3+meet such requirements and are recognized as potential PDT agents. Clinically, Photofrin® PDT in neuroblastoma therapy proved in pediatric subjects diagnosed with progressive/recurrent malignant brain tumors increased progression free survival and overall survival outcome...
October 25, 2016: Oncotarget
Haixia Qiu, Michele M Kim, Rozhin Penjweini, Timothy C Zhu
No abstract text is available yet for this article.
August 1, 2016: Journal of Biomedical Optics
David Kessel
Photodynamic therapy (PDT) offers a new approach to selective tumor eradication. Modifications designed to increase and optimize efficacy continue to emerge. Selective photodamage to malignant cells and their environment can bring about tumor cell destruction, shutdown of the tumor vasculature, stimulation of immunologic anti-tumor effects and potentiation of other therapeutic effects. Current development of combination protocols may provide a better rationale for integration of PDT into clinical practice. An example described here is the ability of a sequential (two-sensitizer) PDT protocol to enhance the efficacy of photokilling...
January 2016: Journal of Porphyrins and Phthalocyanines
Nathan R Gemmell, Aongus McCarthy, Michele M Kim, Israel Veilleux, Timothy C Zhu, Gerald S Buller, Brian C Wilson, Robert H Hadfield
This paper presents a novel compact fiberoptic based singlet oxygen near-infrared luminescence probe coupled to an InGaAs/InP single photon avalanche diode (SPAD) detector. Patterned time gating of the single-photon detector is used to limit unwanted dark counts and eliminate the strong photosensitizer luminescence background. Singlet oxygen luminescence detection at 1270 nm is confirmed through spectral filtering and lifetime fitting for Rose Bengal in water, and Photofrin in methanol as model photosensitizers...
February 2017: Journal of Biophotonics
Wenli Xiong, Xiaobing Wang, Jianmin Hu, Yichen Liu, Quanhong Liu, Pan Wang
Sinoporphyrin sodium (DVDMS) is a newly identified photosensitizer that was isolated from Photofrin. Experimental and clinical results have demonstrated that repeated application of PDT greatly improved the therapeutic efficacy. Here, we comparatively studied two kinds of photodynamic therapy (PDT) strategies by using DVDMS (2mg/kg) in murine breast cancer 4T1 xenograft model to provide evidence which strategy exerts a better antitumor effect. Regimen (1): DVDMS was injected one time into tumor-bearing mice, which were then repeatedly exposed to 50J/cm(2) light 24h, 30h and 36h later...
July 2016: Journal of Photochemistry and Photobiology. B, Biology
Michele M Kim, Rozhin Penjweini, Nathan R Gemmell, Israel Veilleux, Aongus McCarthy, Gerald Buller, Robert H Hadfield, Brian C Wilson, Timothy C Zhu
An explicit dosimetry model has been developed to calculate the apparent reacted (1) O 2 concentration ([(1) O 2]rx) in an in-vivo model. In the model, a macroscopic quantity, g, is introduced to account for oxygen perfusion to the medium during PDT. In this study, the SOED model is extended for PDT treatment in phantom conditions where vasculature is not present; the oxygen perfusion is achieved through the air-phantom interface instead. The solution of the SOED model is obtained by solving the coupled photochemical rate equations incorporating oxygen perfusion through the air-liquid interface...
February 13, 2016: Proceedings of SPIE
Michele M Kim, Arash Darafsheh, Mahmoud Ahmad, Jarod C Finlay, Timothy C Zhu
PDT dose is the product of the photosensitizer concentration and the light fluence in the target tissue. For improved dosimetry during plural photodynamic therapy (PDT), a PDT dose dosimeter was developed to measure both the light fluence and the photosensitizer concentration simultaneously in the same treatment location. Light fluence and spectral data were rigorously compared to other methods of measurement (e.g. photodiode, multi-fiber spectroscopy contact probe) to assess the accuracy of the measurements as well as their uncertainty...
March 17, 2016: Proceedings of SPIE
B O Sun, Wei Li, Ning Liu
Advanced colorectal cancer has a high mortality rate and conventional treatments have poor therapeutic effects. The aim of the present study was to analyze the recent curative effect and adverse reaction of photofrin photodynamic adjuvant treatment on young patients with advanced colorectal cancer. A total of 23 patients with advanced colorectal cancer who had accepted semiconductor laser photodynamic adjuvant treatment were selected as the observation group. In addition, 30 patients who had accepted concurrent radiotherapy and chemotherapy during the same period served as the control group...
March 2016: Oncology Letters
Muthusamy Selvaganapathy, Narayanaperumal Pravin, Vellaichamy Muniyandi, Mohammed Nazeer, Natarajan Raman
A new series of cysteine-based metal(II) complexes with 2,2'-bipyridine or 1,10-phenanthroline as co-ligand have been prepared and characterized. Their DNA binding and cleavage properties have been studied. The analytical and spectroscopic data of complexes 1-18 reveal that the complexes adopt an octahedral geometry around the central metal ion in which the cysteine is coordinated through NS and NN atoms, respectively. Spectroscopic titration and viscosity measurements reveal that the complexes bind to DNA through an intercalative mode...
April 2016: Journal of Photochemistry and Photobiology. B, Biology
Shegan Gao, Shuo Liang, Kaili Ding, Zhifeng Qu, Ying Wang, Xiaoshan Feng
Photodynamic therapy (PDT), which uses a light-sensitive compound and laser irradiation, is a light-based oncological treatment modality. PDT offers an alternative, less invasive treatment for various malignant tumors, such as esophageal cancer (EC), through a photochemical reaction induced by photofrin-II or other oncotropic photosensitizers without severe complications. Previous studies has shown that cancerous tissues accumulated more photosensitizers than paired normal tissues, however, whether it is cellular or vascular mechanisms remains unknown...
June 2016: Photodiagnosis and Photodynamic Therapy
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