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https://www.readbyqxmd.com/read/28350556/resorbable-plates-prevent-regression-in-pediatric-mandibular-distraction-osteogenesis
#1
Brandon Kikuo Richland, Christopher Ellstrom, Asim Ahmad, Daniel Jaffurs
BACKGROUND: Mandibular distraction osteogenesis is an important technique to correct the pediatric hypoplastic mandible. Regression of the mandible after distraction continues to be a challenge, with some studies reporting up to 22% to 56% decrease toward predistraction length in the first year. We hypothesize that a resorbable plate placed after removal of the distractor will provide additional stability to newly formed bone, allowing further time for consolidation and minimizing regression...
March 27, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28350548/pelvic-discontinuity-associated-with-total-hip-arthroplasty-evaluation-and-management
#2
Matthew P Abdel, Robert T Trousdale, Daniel J Berry
Pelvic discontinuity is a challenging complication encountered during revision total hip arthroplasty. Pelvic discontinuity is defined as a separation of the ilium superiorly from the ischiopubic segment inferiorly and is typically a chronic condition in failed total hip arthroplasties in the setting of bone loss. After a history and a physical examination have been completed and infection has been ruled out, appropriate imaging must be obtained, including plain hip radiographs, oblique Judet radiographs, and often a CT scan...
March 27, 2017: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/28346918/5-ht1a-receptors-mediate-analgesia-induced-by-emulsified-sevoflurane-in-thermal-nociception-but-have-little-effect-on-chemical-nociception
#3
Chen Yan, Dai Ti-Jun, Li Xin, Cao Gao, Jiang Shen, Tao Hong, Meng Zhi-Xiu
OBJECTIVE: The study aimed to investigate the relationship between the analgesic effect of sevoflurane and 5-serotonin receptor 1A (5-HT1A R) in the spinal cords of mice. METHODS: Analgesic mouse models were established by intraperitoneal injection of emulsified sevoflurane, and the influence of p-MPPF (a specific antagonist of 5-HT1A Rs) intrathecal injection on the changes in tail-flick latency in tail-withdrawal test, pain threshold in hot-plate test (HPPT), and writhing times in acetic acid-induced writhing test were recorded...
March 28, 2017: Pharmacology
https://www.readbyqxmd.com/read/28346890/high-throughput-system-wide-engineering-and-screening-for-microbial-biotechnology
#4
REVIEW
Yannick Vervoort, Alicia Gutiérrez Linares, Miguel Roncoroni, Chengxun Liu, Jan Steensels, Kevin J Verstrepen
Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their experiments to the nanoliter and picoliter scale and the single-cell level. In this review, we summarize different high-throughput system-wide genome engineering and screening strategies for microbes...
March 24, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28346828/chemical-transformation-of-colloidal-nanostructures-with-morphological-preservation-by-surface-protection-with-capping-ligands
#5
Wenjing Xu, Mingsheng Wang, Zhiwei Li, Xiaojing Wang, Yongqiang Wang, Mingyang Xing, Yadong Yin
When nanocrystals are made to undergo chemical transformations, there are often accompanying large mechanical deformations and changes to overall particle morphology. These effects can constrain development of multistep synthetic methods through loss of well-defined particle morphology and functionality. Here, we demonstrate a surface protection strategy for solution phase chemical conversion of colloidal nanostructures that allows for preservation of overall particle morphology despite large volume changes...
March 28, 2017: Nano Letters
https://www.readbyqxmd.com/read/28346810/an-automated-multiplexed-hepatotoxicity-and-cyp-induction-assay-using-heparg-cells-in-2d-and-3d
#6
Lindsey M Ott, Karthik Ramachandran, Lisa Stehno-Bittel
Drug-induced liver injury (DILI) and drug-drug interactions (DDIs) are concerns when developing safe and efficacious compounds. We have developed an automated multiplex assay to detect hepatotoxicity (i.e., ATP depletion) and metabolism (i.e., cytochrome P450 1A [CYP1A] and cytochrome P450 3A4 [CYP3A4] enzyme activity) in two-dimensional (2D) and three-dimensional (3D) cell cultures. HepaRG cells were cultured in our proprietary micromold plates and produced spheroids. HepaRG cells, in 2D or 3D, expressed liver-specific proteins throughout the culture period, although 3D cultures consistently exhibited higher albumin secretion and CYP1A/CYP3A4 enzyme activity than 2D cultures...
March 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28346795/understanding-the-emission-impacts-of-hov-to-hot-lane-conversions-experience-from-atlanta-ga
#7
Yanzhi Ann Xu, Haobing Liu, Michael O Rodgers, Angshuman Guin, Michael Hunter, Adnan Sheikh, Randall L Guensler
Converting a congested High Occupancy Vehicle (HOV) lane into a High Occupancy Toll (HOT) lane is a viable option for improving travel time reliability for carpools and buses that use the managed lane. However, the emission impacts of HOV-to-HOT-conversions are not well understood. The lack of emissions impact quantification for HOT conversions creates a policy challenge for agencies making transportation funding choices. The goal of this paper is to evaluate the case study before-and-after changes in vehicle emissions for the Atlanta, GA I-85 HOV/HOT lane conversion project, implemented in October, 2011...
March 27, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28346437/friction-forces-position-the-neural-anlage
#8
Michael Smutny, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht, Daniel Čapek, Martin Behrndt, Ekaterina Papusheva, Masazumi Tada, Björn Hof, Tamás Vicsek, Guillaume Salbreux, Carl-Philipp Heisenberg
During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage...
March 27, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28346098/filter-plate-based-screening-of-mip-spe-materials-for-capture-of-the-biomarker-pro-gastrin-releasing-peptide
#9
Kishore Kumar Jagadeesan, Cecilia Rossetti, Abed Abdel Qader, Léon Reubsaet, Börje Sellergren, Thomas Laurell, Simon Ekström
Affinity-based solid-phase extraction (SPE) is an attractive low-cost sample preparation strategy for biomarker analysis. Molecularly imprinted polymers (MIPs) as affinity sorbents offer unique opportunities for affinity SPE, due to their low manufacturing cost and high robustness. A limitation is the prediction of their affinity; therefore, screening of analyte recovery and specificity within a large range of SPE conditions is important in order to ensure high-sensitivity detection and assay reproducibility...
January 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28346096/a-novel-multiparametric-drug-scoring-method-for-high-throughput-screening-of-3d-multicellular-tumor-spheroids-using-the-celigo-image-cytometer
#10
Scott Cribbes, Sarah Kessel, Scott McMenemy, Jean Qiu, Leo Li-Ying Chan
Three-dimensional (3D) tumor models have been increasingly used to investigate and characterize cancer drug compounds. The ability to perform high-throughput screening of 3D multicellular tumor spheroids (MCTS) can highly improve the efficiency and cost-effectiveness of discovering potential cancer drug candidates. Previously, the Celigo Image Cytometer has demonstrated a novel method for high-throughput screening of 3D multicellular tumor spheroids. In this work, we employed the Celigo Image Cytometer to examine the effects of 14 cancer drug compounds on 3D MCTS of the glioblastoma cell line U87MG in 384-well plates...
January 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28346094/drug-library-screening-for-the-identification-of-ionophores-that-correct-the-mistrafficking-disorder-associated-with-oxalosis-kidney-disease
#11
Shurong Hou, Franck Madoux, Louis Scampavia, Jo Ann Janovick, P Michael Conn, Timothy P Spicer
Primary hyperoxaluria is the underlying cause of oxalosis and is a life-threatening autosomal recessive disease, for which treatment may require dialysis or dual liver-kidney transplantation. The most common primary hyperoxaluria type 1 (PH1) is caused by genetic mutations of a liver-specific enzyme alanine:glyoxylate aminotransferase (AGT), which results in the misrouting of AGT from the peroxisomes to the mitochondria. Pharmacoperones are small molecules with the ability to modify misfolded proteins and route them correctly within the cells, which may present an effective strategy to treat AGT misrouting in PH1 disorders...
January 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28346088/a-1536-well-3d-viability-assay-to-assess-the-cytotoxic-effect-of-drugs-on-spheroids
#12
Franck Madoux, Allison Tanner, Michelle Vessels, Lynsey Willetts, Shurong Hou, Louis Scampavia, Timothy P Spicer
Evaluation of drug cytotoxicity traditionally relies on use of cell monolayers, which are easily miniaturized to the 1536-well plate format. Three-dimensional (3D) cell culture models have recently gained popularity thanks to their ability to better mimic the complexity of in vivo systems. Despite growing interest in these more physiologically relevant and highly predictive cell-based models for compound profiling and drug discovery, 3D assays are currently performed in a medium- to low-throughput format, either in 96-well or 384-well plates...
January 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28345923/geometry-and-topology-of-2d-dry-foams-computer-simulation-and-experimental-characterisation
#13
Mingming Tong, Katie Cole, Pablo R Brito-Parada, Stephen Johan Neethling, Jan J Cilliers
Pseudo-2D foams are commonly used in foam studies as it is experimentally easier to measure the bubble size distribution and other geometric and topological properties of these foams than it is for a 3D foam. Despite the widespread use of 2D foams in both simulation and experimental studies, many important geometric and topological relationships are still not well understood. Film size, for example, is a key parameter in the stability of bubbles and the overall structure of foams. The relationship between the size distribution of the films in a foam and that of the bubbles themselves is thus a key relationship in the modelling and simulation of unstable foams...
March 27, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28345610/early-loss-of-subchondral-bone-following-microfracture-is-counteracted-by-bone-marrow-aspirate-in-a-translational-model-of-osteochondral-repair
#14
Liang Gao, Patrick Orth, Kathrin Müller-Brandt, Lars K H Goebel, Magali Cucchiarini, Henning Madry
Microfracture of cartilage defects may induce alterations of the subchondral bone in the mid- and long-term, yet very little is known about their onset. Possibly, these changes may be avoided by an enhanced microfracture technique with additional application of bone marrow aspirate. In this study, full-thickness chondral defects in the knee joints of minipigs were either treated with (1) debridement down to the subchondral bone plate alone, (2) debridement with microfracture, or (3) microfracture with additional application of bone marrow aspirate...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345446/inter-species-comparative-antioxidant-assay-and-hptlc-analysis-of-sakuranetin-in-the-chloroform-and-ethanol-extracts-of-aerial-parts-of-rhus-retinorrhoea-and-rhus-tripartita
#15
Perwez Alam, Mohammad Khalid Parvez, Ahmed Hassan Arbab, Nasir Ali Siddiqui, Mohammed Salem Al-Dosary, Adnan Jathlan Al-Rehaily, Sarfaraz Ahmed, Mohd Abul Kalam, Mohammad Shamim Ahmad
CONTEXT: Extensive research on Rhus (Anacardiaceae) shows their antioxidant potential, which warrants further evaluation of its other species. OBJECTIVE: To perform a comparative antioxidant assay on extracts of R. retinorrhoea and R. tripartita, including sakuranetin quantification by a validated HPTLC method. MATERIALS AND METHODS: In vitro antioxidant assay was performed on chloroform and ethanol extracts of R. retinorrhoea Steud. ex Oliv...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28345372/screening-of-intestinal-crypt-organoids
#16
Svenja Ley, Olaf Galuba, Adrian Salathe, Nicolas Melin, Alexandra Aebi, Monika Pikiolek, Judith Knehr, Walter Carbone, Martin Beibel, Florian Nigsch, Guglielmo Roma, Giovanni d'Ario, Susan Kirkland, Laure C Bouchez, Caroline Gubser Keller, Tewis Bouwmeester, Christian N Parker, Heinz Ruffner
Oral and intestinal mucositis is a debilitating side effect of radiation treatment. A mouse model of radiation-induced mucositis leads to weight loss and tissue damage, reflecting the human ailment as it responds to keratinocyte growth factor (KGF), the standard-of-care treatment. Cultured intestinal crypt organoids allowed the development of an assay monitoring the effect of treatments of intestinal epithelium to radiation-induced damage. This in vitro assay resembles the mouse model as KGF and roof plate-specific spondin-1 (RSPO1) enhanced crypt organoid recovery following radiation...
January 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28345364/anatomically-contoured-anterior-plating-for-isolated-tibiotalar-arthrodesis
#17
Nicholas Kusnezov, John C Dunn, Logan R Koehler, Justin D Orr
INTRODUCTION: We performed a systematic review of the published literature to characterize patient demographic, surgical techniques, and functional outcomes to elucidate the complication and revision rates following isolated tibiotalar arthrodesis with anatomically contoured anterior plating. METHODS: A comprehensive literature search was performed. Inclusion criteria were peer-reviewed studies in English, after 1990, at least 10 patients, and reporting clinical outcomes following contoured anterior plating and with follow-up of at least 80% and 1 year...
March 1, 2017: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/28344744/automation-of-pacbio-smrtbell-ngs-library-preparation-for-bacterial-genome-sequencing
#18
Nguyet Kong, Whitney Ng, Kao Thao, Regina Agulto, Allison Weis, Kristi Spittle Kim, Jonas Korlach, Luke Hickey, Lenore Kelly, Stephen Lappin, Bart C Weimer
BACKGROUND: The PacBio RS II provides for single molecule, real-time DNA technology to sequence genomes and detect DNA modifications. The starting point for high-quality sequence production is high molecular weight genomic DNA. To automate the library preparation process, there must be high-throughput methods in place to assess the genomic DNA, to ensure the size and amounts of the sheared DNA fragments and final library. FINDINGS: The library construction automation was accomplished using the Agilent NGS workstation with Bravo accessories for heating, shaking, cooling, and magnetic bead manipulations for template purification...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28344726/evaluation-of-disposable-microfluidic-chip-design-for-automated-and-fast-immunoassays
#19
Guochun Wang, Champak Das, Bradley Ledden, Qian Sun, Chien Nguyen, Sai Kumar
We report here, the design and development of a disposable immunoassay chip for protein biomarker detection within ∼1 h. The unique design allows for real-time dynamic calibration of immunoassay for multiple biomarker detections on the chip. The limit of detection achieved for this test chip is 10 pg/ml for IL6, and 50 pg/ml for GFAP with a detection time of 1 h. The prototype instrument used for flowing the reagents through the chip can be easily assembled from off-the-shelf components with the final chemiluminescent detection carried out in a commercial plate reader...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28343921/cell-growth-of-immortalized-arachnoid-cells-in-the-presence-of-fibroblasts-and-blood-products
#20
Catherine A Miller, Eric A Hansen, Cornelius H Lam
OBJECT: The pathophysiology of non-obstructive hydrocephalus involves alteration in cerebrospinal fluid (CSF) pathways. The exact mechanism is unknown, but as arachnoid CSF egress is a major route of CSF removal, damage or alteration to the growth of arachnoid cells may influence the rate of CSF absorption. We investigated the effect of soluble factors secreted by fibroblasts and the presence of blood products on arachnoid cell growth. METHODS: An immortalized arachnoid cell line was developed and cells were grown on semipermeable membranes in a culture chamber...
March 23, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
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