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https://www.readbyqxmd.com/read/29356416/a-neuropathological-study-of-novel-rttn-gene-mutations-causing-a-familial-microcephaly-with-simplified-gyral-pattern
#1
Suzanne Chartier, Caroline Alby, Lucile Boutaud, Sophie Thomas, Nadia Elkhartoufi, Jelena Martinovic, Josseline Kaplan, Alexandra Benachi, Didier Lacombe, Pascale Sonigo, Séverine Drunat, Michel Vekemans, Joël Agenor, Férechté Encha Razavi, Tania Attie-Bitach
BACKGROUND: The RTTN gene encodes Rotatin, a large centrosomal protein involved in ciliary functions. RTTN mutations have been reported in seven families and are associated with two phenotypes: polymicrogyria associated with seizures and primary microcephaly associated with primordial dwarfism. CASE: A targeted exome sequencing of morbid genes causing cerebral malformations identified novel RTTN compound heterozygous mutations in a family where three pregnancies were terminated because a severe fetal microcephaly was diagnosed...
January 22, 2018: Birth Defects Research
https://www.readbyqxmd.com/read/29356310/adaptive-evolution-in-the-coccolithophore-gephyrocapsa-oceanica-following-1000-generations-of-selection-under-elevated-co2
#2
Shanying Tong, Kunshan Gao, David A Hutchins
Coccolithophores are important oceanic primary producers not only in terms of photosynthesis, but also because they produce calcite plates called coccoliths. Ongoing ocean acidification associated with changing seawater carbonate chemistry may impair calcification and other metabolic functions in coccolithophores. While short-term ocean acidification effects on calcification and other properties have been examined in a variety of coccolithophore species, long-term adaptive responses have scarcely been documented, other than for the single species Emiliania huxleyi...
January 22, 2018: Global Change Biology
https://www.readbyqxmd.com/read/29356309/direct-assessment-of-the-toxicity-of-molybdenum-disulfide-atomically-thin-film-and-microparticles-via-cytotoxicity-and-patch-testing
#3
Weibing Chen, Wenjin Qi, Wei Lu, Nikhil Roy Chaudhury, Jiangtan Yuan, Lidong Qin, Jun Lou
The low toxicity of molybdenum disulfide (MoS2 ) atomically thin film and microparticles is confirmed via cytotoxicity and patch testing in this report. The toxicity of MoS2 thin film and microparticles is extensively studied but is still inconclusive due to potential organic contamination in the preparations of samples. Such contamination is avoided here through preparing MoS2 atomically thin film via direct sulfurization of molybdenum thin film on quartz plate, which permits a direct assessment of its toxicity without any contamination...
January 22, 2018: Small
https://www.readbyqxmd.com/read/29356276/influence-of-breathing-patterns-and-orthostatic-stress-on-postural-control-in-older-adults
#4
Gabriel D Rodrigues, Jonas L Gurgel, Thiago R Gonçalves, Flávia Porto, Pedro Paulo da S Soares
AIM: To investigate the influence of breathing patterns and blood pressure behavior postural control in older adults. METHODS: A total of 20 older adults carried out spontaneous, controlled (15 cycles/min) and deep (6 cycles/min) breathing trials, in random order. In this session, the heart rate was recorded continuously and blood pressure measured every 3 min. In addition, the challenge from sitting to an active standing position was carried out with eyes closed during 6 min on a baropodometer plate with electromyography recording...
January 22, 2018: Geriatrics & Gerontology International
https://www.readbyqxmd.com/read/29356220/comparison-of-false-negative-rates-and-limits-of-detection-following-macrofoam-swab-sampling-of-bacillus-anthracis-surrogates-via-rapid-viability-pcr-and-plate-culture
#5
Janine R Hutchison, Greg F Piepel, Brett G Amidan, Becky M Hess, Michael A Sydor, Brooke L Deatherage Kaiser
AIMS: We evaluated the effects of Bacillus anthracis surrogates, low surface concentrations, surface materials, and assay methods on false-negative rate (FNR) and limit of detection (LOD95 ) for recovering Bacillus spores using a macrofoam-swab sampling procedure. METHODS AND RESULTS: Bacillus anthracis Sterne or Bacillus atrophaeus Nakamura spores were deposited over a range of low target concentrations (2 - 500 coupon-1 ) onto glass, stainless steel, vinyl tile, and plastic...
January 21, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29356062/mouldable-thermoplastic-glue-on-frog-supportive-shoes-change-hoof-kinetics-in-normal-and-obese-shetland-ponies
#6
J Sleutjens, F M Serra Bragança, M W van Empelen, R E Ten Have, J de Zwaan, E Roelfsema, M Oosterlinck, W Back
BACKGROUND: Obesity and hyperinsulinaemia are frequently encountered in the equine population and risk factors for the development of laminitis. There are many options for hoof support that claim a beneficial effect, but often the scientific evidence is scarce. OBJECTIVES: To quantify the effect of frog-supportive shoes on hoof kinetics in normal and obese ponies. STUDY DESIGN: Controlled in vivo trial. METHODS: Ten Shetland mares (n = 10) with a normal (n = 5) or obese (n = 5) body condition were led over a dynamically calibrated pressure plate before (T0), immediately after (T1), and 72 hours (T2) after application of the shoes...
January 21, 2018: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29355699/safety-hazards-in-bacteriocinogenic-staphylococcus-strains-isolated-from-goat-and-sheep-milk
#7
Samane Rahmdel, Saeid Hosseinzadeh, Seyed Shahram Shekarforoush, Sandra Torriani, Veronica Gatto, Safoora Pashangeh
In this study, 28 bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk were subjected to the PCR detection of enterotoxin genes (sea-see), enterotoxin-like toxin Q gene (selq), toxic shock syndrome toxin gene (tst1), and antibiotic resistance genes. They were also evaluated for phenotypic resistance against 10 antibiotics and hemolytic activity. The tyramine and histamine production was investigated using the agar plate assay and capillary zone electrophoretic analysis (CZE). Twenty-five isolates harbored at least one enterotoxin gene...
January 17, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29355502/the-influence-of-temperature-and-salinity-on-mortality-of-recently-recruited-blue-crabs-callinectes-sapidus-naturally-infected-with-hematodinium-perezi-dinoflagellata
#8
Juan Pablo Huchin-Mian, Hamish J Small, Jeffrey D Shields
The parasitic dinoflagellate Hematodinium perezi is highly prevalent in juvenile blue crabs, Callinectes sapidus, along the eastern seaboard of the USA. Although the parasite is known to kill adult crabs, the mortality rate of naturally infected juvenile crabs remains unknown. We analyzed the influence of temperature and salinity on the mortality of recently recruited blue crab that were naturally infected with H. perezi. Over 492 juvenile crabs (infected, n=282; uninfected controls, n=210) were held individually in six-well plates and held at six temperatures (4, 10, 15, 20, 25, and 30 °C) or three salinities (5, 15, and 30 psu) for a maximum of 90 days...
January 18, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29355416/antimicrobial-activity-of-a-colloidal-agnp-suspension-demonstrated-in-vitro-against-monoculture-biofilms-toward-a-novel-tooth-disinfectant-for-treating-dental-caries
#9
D R Schwass, K M Lyons, R Love, G R Tompkins, C J Meledandri
A novel silver nanoparticle (AgNP) formulation was developed as a targeted application for the disinfection of carious dentine. Silver nitrate (AgNO3) was chemically reduced using sodium borohydrate (NaBH4) in the presence of sodium dodecyl sulfate (SDS) to form micelle aggregate structures containing monodisperse 6.7- to 9.2-nm stabilized AgNPs. AgNPs were characterized by measurement of electrical conductivity and dynamic light scattering, scanning electron microscopy, transmission electron microscopy, and inductively coupled plasma mass spectrometry...
February 2018: Advances in Dental Research
https://www.readbyqxmd.com/read/29355311/unexpected-behavior-of-np-in-oxo-selenate-oxo-selenite-systems
#10
Eike M Langer, Olaf Walter, Jean-Yves Colle, Dirk Bosbach, Evgeny V Alekseev
A study of neptunium (Np) chemistry in the complex oxo-selenium system has been performed. Hereby, two sets of precipitation experiments were conducted, investigating the influence of the initial oxidation state of selenium using SeIVO2 and H2SeVIO4 with NpV in alkali nitrate solution, keeping the ratio of Np/Se constant. Surprising results were observed. Five novel neptunium and selenium bearing compounds have been obtained by slow evaporation from aqueous solution. The novel NpIV phase K4-x[Np(SeO3)4-x(HSeO3)x]·(H2O)1...
January 22, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29354775/in-vitro-antimicrobial-effect-of-different-root-canal-sealers-against-oral-pathogens
#11
A Monajemzadeh, S Ahmadi Asoor, S Aslani, B Sadeghi-Nejad
Background and Purpose: Root canal therapy is the primary method for the treatment of an infected pulp in modern dentistry. The main aim of endodontic treatment is the elimination of bacteria and their products from infected root canals. In this study, we attempted to investigate the antimicrobial activity of three root canal sealers against oral pathogens. Materials and Methods: The antimicrobial effectiveness of three endodontic sealers with different chemical compositions, namely resin (AH 26), zinc oxide eugenol (ZOE), and mineral trioxide aggregate (MTA), against Candida albicans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans, and Lactobacillus casei was assayed by agar well diffusion method (AWDM)...
June 2017: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/29354737/a-radiographic-follow-up-study-of-stand-alone-cage-and-graft-plate-constructs-for-single-level-anterior-cervical-discectomy-and-fusion
#12
Joseph F Baker, Jaime Gomez, Kartik Shenoy, Sarah Kim, Afshin Razi, Yong Kim
Background: Anterior cervical discectomy and fusion (ACDF) may be performed using an interbody cage or graft with an anterior plate or with a stand-alone (SA) interbody device without the anterior plate. The pros and cons of each vary. This study examined the radiographic outcome of the two techniques with a focus on implant subsidence. Methods: A retrospective review of cases of singe level ACDF by a single surgeon was undertaken. Medical and radiographic records were reviewed to determine subsidence, pre- and post-operative segmental and total lordosis in cohorts of both stand-alone and graft-and-plate constructs...
December 2017: Journal of Spine Surgery (Hong Kong)
https://www.readbyqxmd.com/read/29354171/the-challenge-of-nonunion-after-osteosynthesis-of-the-clavicle-is-it-a-biomechanical-or-infection-problem
#13
Giuseppe Rollo, Paolo Pichierri, Antonio Marsilio, Marco Filipponi, Michele Bisaccia, Luigi Meccariello
Introduction: The nonunion rate has been reported between 0.1% and 15%. There are also several predisposing factors for the onset of complications: general factors connected with the patient and specific factors related to the fracture site. The purpose of our study is to review the etiology of nonunion of the clavicle in its atrophic form and investigate the outcomes of the revision treatment in a single step. Materials and methods: Retrospective study on 71 patients suffering from nonunions due to the following treatments: conservative in 13 patients; plate fixation in 12; closed reduction and fixation with K-wire in 24; open reduction and fixation with K-wire...
September 2017: Clinical Cases in Mineral and Bone Metabolism
https://www.readbyqxmd.com/read/29354157/grafting-and-fixation-of-proximal-humeral-aseptic-non-union-a-prospective-case-series
#14
Giuseppe Rollo, Roberto Rotini, Paolo Pichierri, Marco Giaracuni, Alessandro Stasi, Luca Macchiarola, Michele Bisaccia, Luigi Meccariello
Background: Fractures of the proximal part of the humerus represent almost 4-5% of all fractures. The rate of non union is estimated to be 1.1 to 10%. Non union, displacement, and fixation failure can be hazardous complications for these injuries. The purpose of our study was to evaluate the outcomes of plate and bone strut allograft with bone chips grafting augmentation in the management of proximal humeral aseptic non union. Methods: We treated 16 aseptic non union proximal humeral fractures by the medial humeral shaft bone strut allograft and lateral plate and screws with bone chips grafting...
September 2017: Clinical Cases in Mineral and Bone Metabolism
https://www.readbyqxmd.com/read/29354155/the-effect-of-strontium-chloride-on-human-periodontal-ligament-stem-cells
#15
Cecilia Romagnoli, Roberto Zonefrati, Gianna Galli, Alessandra Aldinucci, Niccolò Nuti, Francesco Saverio Martelli, Paolo Tonelli, Annalisa Tanini, Maria Luisa Brandi
The complete repair of periodontal structures remains an exciting challenge that prompts researchers to develop new treatments to restore the periodontium. Recent research has suggested strontium ion to be an attractive candidate to improve osteogenic activity. In this study, we have isolated a clonal finite cell line derived from human periodontal ligament (PDL) in order to assess whether and in which way different doses of SrCl2 (from 0.5 to 500 μg/ml) can influence both the proliferation and the mineralization process, for future application in oral diseases...
September 2017: Clinical Cases in Mineral and Bone Metabolism
https://www.readbyqxmd.com/read/29353892/magnetic-resonance-imaging-findings-in-spondylodiscitis
#16
Om Biju Panta, Yagya Raj Pathak, Dan Bahadur Karki
BACKGROUND: Magnetic Resonance Imaging is the imaging modality of choice for imaging spinal infection due to its high sensitivity and specificity. This study aims to study the magnetic resonance imaging changes in patients with spondylodiscitis. METHODS: The study was a retrospective study carried in a multimodality imaging centre in Kathmandu. Magnetic resonance imaging records and clinical record of 3 years duration were reviewed and patients with clinical and radiological diagnosis of spondylodiscitis were included in the study...
January 1, 2018: Journal of Nepal Health Research Council
https://www.readbyqxmd.com/read/29353526/using-3-dimensional-modeling-to-customize-titanium-plates-for-repair-of-chest-wall-trauma
#17
Justin A Smith, Vanessa P Ho, Christopher W Towe
BACKGROUND: Open reduction and internal fixation of rib fractures is recommended to decrease mortality, shorten the duration of mechanical ventilation, and lower hospital length of stay. Prosthetic titanium plates are frequently used to repair chest wall trauma, and are typically contoured to the patient's anatomy at the time of implant in the operating room. We describe the use of 3-dimensional (3D) digitally corrected rapid prototyping to generate a model of a patient's skeletal anatomy for the purposes of preoperative customization of standard titanium plates for fixation of rib fractures...
January 1, 2018: Surgical Innovation
https://www.readbyqxmd.com/read/29353523/acromioclavicular-joint-disruptions-a-comparison-of-two-surgical-approaches-hook-and-rope
#18
M S Athar, Neil Ashwood, Georgios Arealis, Mark Hamlet, Emma Salt
INTRODUCTION: Acromioclavicular joint injuries are common shoulder girdle injuries most commonly resulting from a direct blow to the acromion with the arm adducted. Type-I or type-II acromioclavicular joint injuries can be managed with sling immobilization, early shoulder motion, and physiotherapy. The management of type-III injuries remains controversial and is individualized. Type IV and V injuries should be treated surgically. A myriad of surgical techniques for the management of acromioclavicular joint injuries have been reported...
January 2018: Journal of Orthopaedic Surgery
https://www.readbyqxmd.com/read/29353148/light-and-electron-microscopic-observations-on-the-organization-of-skin-and-associated-glands-of-two-caecilian-amphibians-from-western-ghats-of-india
#19
Arun Damodaran, Beyo Reston Saroja, Ramachandran Kotharambath, Akbarsha Mohammad Abdulkader, Oommen V Oommen, Divya Lekha
We adopted light and electron microscopy to understand the structure of the skin of two species of caecilians, Ichthyophis tricolor and Uraeotyphlus cf. oxyurus, from Western Ghats of Kerala, India. The surface of the skin of these caecilians contains an irregular pattern of microridges. Oval, round and polymorphic glandular openings are randomly distributed all over the skin surface. Most of the openings are funnel shaped. The epithelial cells along the rim of the opening descend into the tunnel of the duct...
January 9, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29353123/a-locking-compression-plate-versus-the-gold-standard-non-locking-plate-with-lag-screw-for-first-metatarsophalangeal-fusion-a-biomechanical-comparison
#20
Daniel Mandell, John Karbassi, Hanbing Zhou, Brian Burroughs, Philip Aurigemma, Abhay R Patel
INTRODUCTION: The treatment of end-stage first metatarso-phalangeal joint (MTP) arthritis has been arthrodesis. A dorsal non-locking plate with a lag screw has been the standard traditional fixation method. This study compares the biomechanical strength of a locking compression plate (LCP) with and without internal compression versus this known gold standard. METHODS: In group 1, six matched pairs of cadaver great toes were used to compare the standard non-locking dorsal plate and 3...
November 4, 2017: Foot
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