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https://www.readbyqxmd.com/read/29151023/quadriceps-force-and-anterior-tibial-force-occur-obviously-later-than-vertical-ground-reaction-force-a-simulation-study
#1
Ryo Ueno, Tomoya Ishida, Masanori Yamanaka, Shohei Taniguchi, Ryohei Ikuta, Mina Samukawa, Hiroshi Saito, Harukazu Tohyama
BACKGROUND: Although it is well known that quadriceps force generates anterior tibial force, it has been unclear whether quadriceps force causes great anterior tibial force during the early phase of a landing task. The purpose of the present study was to examine whether the quadriceps force induced great anterior tibial force during the early phase of a landing task. METHODS: Fourteen young, healthy, female subjects performed a single-leg landing task. Muscle force and anterior tibial force were estimated from motion capture data and synchronized force data from the force plate...
November 18, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29150912/sustaining-an-efficient-and-effective-cho-cell-line-development-platform-by-incorporation-of-24-deep-well-plate-screening-and-multivariate-analysis
#2
Alessandro Mora, Sheng Sam Zhang, Gerald Carson, Bernard Nabiswa, Patrick Hossler, Seongkyu Yoon
Efficient and effective cell line screening is paramount towards a successful biomanufacturing program. Here we describe the implementation of 24-deep well plate screening of CHO lines as part of the cell line development platform at AbbVie. Incorporation of this approach accelerated the identification of the best candidate lines for process development. In an effort to quantify and predict process performance comparability, we compared cell culture performance in 24-deep well plates (24-DWP) and in shake flasks, for a panel of Chinese Hamster Ovary cell lines expressing a monoclonal antibody...
November 18, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29150709/simultaneous-detection-of-nadph-consumption-and-h2o2-production-using-the-ampliflu%C3%A2-red-assay-for-screening-of-p450-activities-and-uncoupling
#3
Lisa K Morlock, Dominique Böttcher, Uwe T Bornscheuer
Cytochrome P450s belong to a large and diverse group of heme-containing enzymes. These monooxygenases catalyze the incorporation of a single atom of molecular oxygen into their substrate. In contrast to most other enzymes, the activity of P450 enzymes is not only dependent on substrate and cofactor availability and reaction conditions, but also depends on the coupling efficiency of the catalytic cycle itself. Through the electron transfer from NAD(P)H to the heme-center of the P450, the enzyme becomes activated and binds oxygen...
November 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29150652/evidence-for-frozen-melts-in-the-mid-lithosphere-detected-from-active-source-seismic-data
#4
Akane Ohira, Shuichi Kodaira, Yasuyuki Nakamura, Gou Fujie, Ryuta Arai, Seiichi Miura
The interactions of the lithospheric plates that form the Earth's outer shell provide much of the evidentiary basis for modern plate tectonic theory. Seismic discontinuities in the lithosphere arising from mantle convection and plate motion provide constraints on the physical and chemical properties of the mantle that contribute to the processes of formation and evolution of tectonic plates. Seismological studies during the past two decades have detected seismic discontinuities within the oceanic lithosphere in addition to that at the lithosphere-asthenosphere boundary (LAB)...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150399/a-plate-waste-evaluation-of-the-farm-to-school-program
#5
Jaclyn D Kropp, Saul J Abarca-Orozco, Glenn D Israel, David C Diehl, Sebastian Galindo-Gonzalez, Lauren B Headrick, Karla P Shelnutt
OBJECTIVE: To investigate the impacts of the Farm to School (FTS) program on the selection and consumption of fruits and vegetables. DESIGN: Plate waste data were recorded using the visual inspection method before and after implementation of the program. SETTING: Six elementary schools in Florida: 3 treatment and 3 control schools. PARTICIPANTS: A total of 11,262 meal observations of National School Lunch Program (NSLP) participants in grades 1-5...
November 14, 2017: Journal of Nutrition Education and Behavior
https://www.readbyqxmd.com/read/29149992/a-separable-surface-enhanced-raman-scattering-substrate-modified-with-mil-101-for-detection-of-overlapping-and-invisible-compounds-after-thin-layer-chromatography-development
#6
Bin Bin Zhang, Yi Shi, Hui Chen, Qing Xia Zhu, Feng Lu, Ying Wei Li
By coupling surface-enhanced Raman spectroscopy (SERS) with thin-layer chromatography (TLC), a powerful method for detecting complex samples was successfully developed. However, in the TLC-SERS method, metal nanoparticles serving as the SERS-active substrate are likely to disturb the detection of target compounds, particularly in overlapping compounds after TLC development. In addition, the SERS detection of compounds that are invisible under both visible light and UV254/365 after TLC development is still a significant challenge...
January 2, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29149735/carbamazepine-lamotrigine-levetiracetam-and-valproic-acid-in-dried-blood-spots-with-liquid-chromatography-tandem-mass-spectrometry-method-development-and-validation
#7
Camilla Linder, Anna Hansson, Sara Sadek, Lars L Gustafsson, Anton Pohanka
Monitoring of antiepileptic drugs in children with epilepsy require multiple visits at a clinic for blood collection. Dried blood spot sampling is an alternative way of collection, performed at home by self-collection and can save time and costs for patients and family members. The aim was to develop and validate an LC-MS/MS dried blood spot method for carbamazepine, lamotrigine, levetiracetam and valproic acid with the requirements of using standard equipment and material in a routine laboratory setting. Whatman-903 filter paper was utilized, and discs were punched into a 96 well plate with an automated puncher and barcode reading...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29148842/erratum
#8
(no author information available yet)
Yao H, Lu H, Zhao H, et al. Open reduction assisted with an external fixator and internal fixation with calcaneal locking plate for intra-articular calcaneal fractures. Foot Ankle Int. 2017;38(10):1107-1114. (Original DOI: 10.1177/1071100717715908) The above article appeared with the incorrect corresponding author. The correct corresponding author is Huading Lu, PhD. The corrected corresponding author information is below: Corresponding Author: Huading Lu, PhD, Department of Orthopaedics, The Fifth Affiliated Hospital of Sun Yat-sen University, NO...
November 1, 2017: Foot & Ankle International
https://www.readbyqxmd.com/read/29148794/preparation-and-formation-process-of-zn-ii-coordinated-nanovesicles
#9
Wuxiao Ding, Naohiro Kameta, Hiroyuki Minamikawa, Mitsutoshi Masuda, Masaki Kogiso
Mixing a glycylglycine-lipid and zinc acetate has been reported to form novel supramolecular Zn(II)-coordinated nanovesicles in ethanol. In this study, we investigate in detail the formation of nanovesicles by using three lipids at different temperatures and discuss their formation process. The original lipids show extremely low solubilities and appear as plate structures in ethanol. Within a small window of lipid solubility, the formation of lipid-Zn(II) complexes occurs mainly on the solid surfaces of plate structures...
November 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29147852/enhanced-peptide-detection-toward-single-neuron-proteomics-by-reversed-phase-fractionation-capillary-electrophoresis-mass-spectrometry
#10
Sam B Choi, Camille Lombard-Banek, Pablo Muñoz-LLancao, M Chiara Manzini, Peter Nemes
The ability to detect peptides and proteins in single cells is vital for understanding cell heterogeneity in the nervous system. Capillary electrophoresis (CE) nanoelectrospray ionization (nanoESI) provides high-resolution mass spectrometry (HRMS) with trace-level sensitivity, but compressed separation during CE challenges protein identification by tandem HRMS with limited MS/MS duty cycle. Here, we supplemented ultrasensitive CE-nanoESI-HRMS with reversed-phase (RP) fractionation to enhance identifications from protein digest amounts that approximate to a few mammalian neurons...
November 16, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29147714/stable-na-plating-stripping-electrochemistry-realized-by-a-3d-cu-current-collector-with-thin-nanowires
#11
Yanying Lu, Qiu Zhang, Mo Han, Jun Chen
Current collectors have a significant role in Na plating and stripping. Here we demonstrate that a three-dimensional (3D) Cu current collector with thin nanowires (<40 nm) can significantly promote the electrochemical deposition stability of Na via tuning a Na metal plating model, therefore contributing to a high efficiency, long lifespan, and low overpotential during electrode/battery cycling.
November 17, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29147105/depletion-of-stercobilin-in-fecal-matter-from-a-mouse-model-of-autism-spectrum-disorders
#12
Emily R Sekera, Heather L Rudolph, Stephen D Carro, Michael J Morales, Glenna C L Bett, Randall L Rasmusson, Troy D Wood
Introduction: Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders lacking a clinical biomarker for diagnosis. Emerging evidence shows that intestinal microflora from ASD subjects can be distinguished from controls, suggesting metabolite differences due to the action of intestinal microbes may provide a means for identifying potential biomarkers for ASD. Objectives: The aim of this study was to determine if quantitative differences in levels of stercobilin and stercobilinogen, metabolites produced by biological action of intestinal microflora, exist in the fecal matter between an ASD mouse model population and controls...
November 2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/29147103/antibacterial-effects-against-various-foodborne-pathogens-and-sensory-properties-of-yogurt-supplemented-with-panax-ginseng-marc-extract
#13
Su Jin Eom, Ji Eun Hwang, Kee-Tae Kim, Hyun-Dong Paik
Panax ginseng marc is produced from fresh ginseng roots during processing and is generally treated as industrial waste. The primary aim of this study was to improve its utilization in the dairy industry as a potential high-value resource. Yogurt was prepared from 11% skim milk powder, 0.1% pectin, 10% sucrose, and ginseng marc ethanol extract (GME, 0.5% and 1.0%) in milk, and was inoculated with a 0.02% yogurt culture (Lactobacillus acidophilus, Bifidobacterium longum, and Streptococcus thermophilus). After fermentation at 40°C for 6-8 h, the physicochemical properties of samples were analyzed by the AOAC, Kjeldahl, and Soxhlet methods...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29147017/oxidation-behavior-of-graphene-coated-copper-at-intrinsic-graphene-defects-of-different-origins
#14
Jinsung Kwak, Yongsu Jo, Soon-Dong Park, Na Yeon Kim, Se-Yang Kim, Hyung-Joon Shin, Zonghoon Lee, Sung Youb Kim, Soon-Yong Kwon
The development of ultrathin barrier films is vital to the advanced semiconductor industry. Graphene appears to hold promise as a protective coating; however, the polycrystalline and defective nature of engineered graphene hinders its practical applications. Here, we investigate the oxidation behavior of graphene-coated Cu foils at intrinsic graphene defects of different origins. Macro-scale information regarding the spatial distribution and oxidation resistance of various graphene defects is readily obtained using optical and electron microscopies after the hot-plate annealing...
November 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/29146595/the-cartilage-specific-lectin-c-type-lectin-domain-family-3-member-a-clec3a-enhances-tissue-plasminogen-activator-mediated-plasminogen-activation
#15
Daniela Lau, Dzemal Elezagic, Gabriele Hermes, Matthias Mörgelin, Alexander P Wohl, Manuel Koch, Ursula Hartmann, Stefan Höllriegl, Raimund Wagener, Mats Paulsson, Thomas Streichert, Andreas R Klatt
C-type lectin domain family 3 member A (CLEC3A) is a poorly characterized protein belonging to the superfamily of C-type lectins. Its closest homologue tetranectin binds to the kringle 4 domain of plasminogen and enhances its association with tissue plasminogen activator (tPA) thereby enhancing plasmin production, but whether CLEC3A contributes to plasminogen activation is unknown. Here, we recombinantly expressed murine and human full-length CLEC3As as well as truncated forms of CLEC3A in HEK-293 EBNA cells...
November 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29146430/soil-biodiversity-effects-from-field-to-fork
#16
REVIEW
Matthias C Rillig, Anika Lehmann, Johannes Lehmann, Tessa Camenzind, Cornelia Rauh
Our knowledge of soil biodiversity in agriculture in general is currently increasing rapidly. However, almost all studies have stopped with the quantification of soil biodiversity effects on crops at harvest time, ignoring subsequent processes along the agrifood chain until food arrives on our plates. Here we develop a conceptual framework for the study of such postharvest effects. We present the main mechanisms (direct and indirect) via which soil biodiversity can influence crop quality aspects and give examples of how effects at harvest time may become attenuated through postharvest operations and how biodiversity may also affect some of these operations (i...
November 13, 2017: Trends in Plant Science
https://www.readbyqxmd.com/read/29146425/unprecedented-sensitivity-of-the-planar-yeast-estrogen-screen-by-using-a-spray-on-technology
#17
Andreas Schoenborn, Pascal Schmid, Sarah Bräm, Georg Reifferscheid, Marina Ohlig, Sebastian Buchinger
The planar yeast estrogen screen (p-YES) can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. The method combines the separation of sample constituents by thin layer chromatography with the direct detection of estrogenic compounds on the surface of the HPTLC-plate. The previous protocol using the immersion of a normal phase silica HPTLC-plate in a cell suspension for bio-autography resulted in blurred signals due to the accelerated diffusion of compounds on the wet surface of the HPTLC-plate...
November 7, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29146168/bone-marrow-derived-mesenchymal-stromal-cells-enhanced-by-platelet-rich-plasma-maintain-adhesion-to-scaffolds-in-arthroscopic-simulation
#18
Alexander R Hoberman, Carl Cirino, Mary Beth McCarthy, Mark P Cote, Leo Pauzenberger, Knut Beitzel, Augustus D Mazzocca, Felix Dyrna
PURPOSE: To assess the response of bone marrow-derived mesenchymal stromal cells (bMSCs) enhanced by platelet-rich plasma (PRP) in the setting of a normal human tendon (NHT), a demineralized bone matrix (DBM), and a fibrin scaffold (FS) with simulated arthroscopic mechanical washout stress. METHODS: Bone marrow was aspirated from the humeral head and concentrated. BMSCs were counted, plated, and grown to confluence. Cells were seeded onto 3 different scaffolds: (1) NHT, (2) DBM, and (3) FS...
November 13, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29145970/minimally-invasive-posteromedial-percutaneous-plate-osteosynthesis-for-diaphyseal-tibial-fractures-technique-description
#19
Andre Wajnsztejn, Robinson Esteves Santos Pires, Alexandre Leme Godoy Dos Santos, Pedro Jose Labronici, Helio Jorge Alvachian Fernandes, Mario Ferretti
PURPOSE: The aim of this study was to evaluate the feasibility of performing minimally-invasive plate osteosynthesis (MIPO) in tibial fractures using two posteromedial incisions, and to measure the distance between the plate and neurovascular structures. MATERIALS AND METHODS: We performed nine dissections of specimens that were submitted to tibial MIPO with two posteromedial incisions. One locking compression plate (LCP) of 14 to 16 holes was inserted into the submuscular tunnel in a retrograde manner...
October 2017: Injury
https://www.readbyqxmd.com/read/29145846/comparison-of-diagnostic-methods-for-early-interproximal-caries-detection-with-near-infrared-light-transillumination-an-in-vivo-study
#20
Ismail Hakki Baltacioglu, Kaan Orhan
BACKGROUND: Although numerous studies have used digital intraoral imaging, only a few studies have used photo-optical methods for the diagnosis of caries. Moreover, several limitations exist in terms of observers (experience and specialty) and the caries lesion itself. Hence, the aims of this study were to evaluate the diagnostic capability of near-infrared light transillumination (NILT) and PSP-Bitewing radiographs and to compare the interobserver and intraobserver differences in addition to observers' experience level to detect early interproximal caries lesions in vivo...
November 16, 2017: BMC Oral Health
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