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https://www.readbyqxmd.com/read/28806852/generation-of-oligodendrogenic-spinal-neural-progenitor-cells-from-human-induced-pluripotent-stem-cells
#1
Mohamad Khazaei, Christopher S Ahuja, Michael G Fehlings
This unit describes protocols for the efficient generation of oligodendrogenic neural progenitor cells (o-NPCs) from human induced pluripotent stem cells (hiPSCs). Specifically, detailed methods are provided for the maintenance and differentiation of hiPSCs, human induced pluripotent stem cell-derived neural progenitor cells (hiPS-NPCs), and human induced pluripotent stem cell-oligodendrogenic neural progenitor cells (hiPSC-o-NPCs) with the final products being suitable for in vitro experimentation or in vivo transplantation...
August 14, 2017: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/28806841/genetic-polymorphisms-as-predictive-markers-of-response-to-growth-hormone-therapy-in-children-with-growth-hormone-deficiency
#2
Anna Maria Jung, Martin Zenker, Christina Lißewski, Denny Schanze, Stefan Wagenpfeil, Tilman Robert Rohrer
Objective Growth hormone (GH) deficiency (GHD) is commonly treated with recombinant human GH (rhGH). Individual response to rhGH therapy varies widely and there is evidence that variations in growth-related genes, e. g. the GH receptor (GHR) gene, may impact treatment response. We aimed to identify genetic polymorphisms which could serve as predictive markers of response to rhGH therapy. Methods We conducted a genetic analysis of single nucleotide polymorphisms (SNPs) and the GHR exon 3 deletion in 101 paediatric GHD patients receiving rhGH...
August 14, 2017: Klinische Pädiatrie
https://www.readbyqxmd.com/read/28806429/current-concepts-of-bruxism
#3
Daniele Manfredini, Junia Serra-Negra, Fabio Carboncini, Frank Lobbezoo
Bruxism is a common phenomenon, and emerging evidence suggests that biologic, psychologic, and exogenous factors have greater involvement than morphologic factors in its etiology. Diagnosis should adopt the grading system of possible, probable, and definite. In children, it could be a warning sign of certain psychologic disorders. The proposed mechanism for the bruxism-pain relationship at the individual level is that stress sensitivity and anxious personality traits may be responsible for bruxism activities that may lead to temporomandibular pain, which in turn is modulated by psychosocial factors...
August 14, 2017: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/28805958/large-orbital-defect-reconstruction-in-the-setting-of-globe-sparing-maxillectomy-the-titanium-hammock-and-layered-fibula-technique
#4
Samuel J Trosman, Timothy M Haffey, Rafael A Couto, Michael A Fritz
INTRODUCTION: The purpose of our study was to describe a novel technique for reconstruction of orbital defects after maxillectomy using a non-anatomic titanium mesh suspension of orbital contents for both support and volume correction. This construct is then articulated with a layered fibula osteocutaneous free flap that restores orbital rim, zygoma, and maxillary alveolus. We herein present our application of this technique, including refinements over time and long-term outcomes. METHODS: A retrospective review was performed on 12 patients who underwent reconstruction of Brown class III orbitopalatomaxillary defects with extensive orbital involvement (at minimum complete orbital floor and rim absent) with titanium mesh sling and a layered fibula free flap...
August 14, 2017: Microsurgery
https://www.readbyqxmd.com/read/28805882/plant-array-chip-for-the-germination-and-growth-screening-of-arabidopsis-thaliana
#5
Youn-Hee Park, Nayoung Lee, Giltsu Choi, Je-Kyun Park
A screening process for the germination and growth of seed is generally required for plant research. Such a repetitive screening process is costly and time-consuming, and its bulky setup requires a lot of space. In particular, the control of the variables, such as light, nutrients, hormones and temperature, is difficult due to the limited space for incubation. In addition, small seeds such as Arabidopsis thaliana are difficult to handle as they are hundreds of microns in diameter and require a more precisely controllable screening environment...
August 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28805724/electroless-nickel-deposition-for-front-side-metallization-of-silicon-solar-cells
#6
Shu Huei Hsieh, Jhong Min Hsieh, Wen Jauh Chen, Chia Chih Chuang
In this work, nickel thin films were deposited on texture silicon by electroless plated deposition. The electroless-deposited Ni layers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDS), X-ray diffraction analysis (XRD), and sheet resistance measurement. The results indicate that the dominant phase was Ni₂Si and NiSi in samples annealed at 300-800 °C. Sheet resistance values were found to correlate well with the surface morphology obtained by SEM and the results of XRD diffraction...
August 14, 2017: Materials
https://www.readbyqxmd.com/read/28805487/state-of-the-art-nanopathological-diagnostics
#7
Luca Roncati, Giuseppe Barbolini
The nanopathological diagnostics (ND) is an ultra-specialized branch of pathological anatomy aimed to identify the nanoparticles of metallic, semimetallic, or nonmetallic elements in the inorganic particulate matter present inside pathological tissues, even on the nanometer scale. ND exploits an environmental scanning electron microscope, connected to an X-ray microprobe mounted on an energy-dispersive spectrometer. The searching of nanoparticles can be performed on paraffin-embedded material, omitting emissions of black overlay and plating procedures...
August 14, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28804616/zinc-oxide-nanoparticle-reduced-biofilm-formation-and-antigen-43-expressions-in-uropathogenic-escherichiacoli
#8
Ali Shakerimoghaddam, Ezzat A Ghaemi, Ailar Jamalli
OBJECTIVES: This study aimed to investigate the effect of zinc oxide nanoparticles (ZnO-np) on biofilm formation and expression of the flu gene in uropathogenic Escherichia coli (UPEC) strains. MATERIALS AND METHODS: Minimum inhibitory concentration (MIC) of ZnO-np was determined by agar dilution method. The effect of MIC and sub-MIC concentrations of ZnO-np on biofilm formation were determined by microtiter plate assay. The expression level of the flu gene was assessed by Real-Time PCR assay...
April 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28804615/pharmacological-effects-of-a-synthetic-quinoline-a-hybrid-of-tomoxiprole-and-naproxen-against-acute-pain-and-inflammation-in-mice-a-behavioral-and-docking-study
#9
Hossein Hosseinzadeh, Fatemeh Mazaheri, Razieh Ghodsi
OBJECTIVES: In the present study, we investigated the potential anti-nociceptive activity and acute anti-inflammatory effect of a synthetic quinoline compound (2-(4-Methoxyphenyl)benzo[h]quinoline-4-carboxylic acid, QC), possessing structural elements of both naproxen and tomoxiprole drugs. MATERIALS AND METHODS: The anti-nociceptive activity of QC was evaluated using chemical- and thermal-induced nociception models and its acute anti-inflammatory effect was evaluated by xylene-induced ear edema test in mice...
April 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28803887/a-miniaturized-peptidyl-prolyl-isomerase-enzyme-assay
#10
Mirella Vivoli, Julien Renou, Arnaud Chevalier, Isobel H Norville, Suraya Diaz, Christina Juli, Helen Atkins, Ulrike Holzgrabe, Pierre-Yves Renard, Mitali Sarkar-Tyson, Nicholas J Harmer
Prolyl-peptidyl isomerases (PPIases) are enzymes that are found in all living organisms. They form an essential part of the cellular protein folding homeostasis machinery. PPIases are associated with many important human diseases, e.g. cardiovascular disease, cancer and Alzheimer's. The development of novel PPIase inhibitors has been limited by the lack of a rapid, laboratory-based assay for these enzymes, as their substrates and products are challenging to distinguish. A well described continuous assay, coupled with the hydrolysis of a peptide by chymotrypsin is highly effective, but comparatively slow...
August 10, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28803160/differentiate-low-impedance-media-in-closed-steel-tank-using-ultrasonic-wave-tunneling
#11
Chunying Wang, Zhaojiang Chen, Wenwu Cao
Ultrasonic wave tunneling through seriously mismatched media, such as steel and water, is possible only when the frequency matches the resonance of the steel plate. But it is nearly impossible to realize continuous wave tunneling if the low acoustic impedance media is air because the transducer frequency cannot be made so accurate. The issue might be resolved using tone-burst signals. Using finite element simulations, we found that for air media when the cycle number is 20, the -6dB bandwidth of energy transmission increased from 0...
August 2, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28803116/efficient-molecularly-imprinted-polymer-as-a-pipette-tip-solid-phase-sorbent-for-determination-of-carvedilol-enantiomers-in-human-urine
#12
Anny Talita Maria da Silva, Hanna Leijoto de Oliveira, Camilla Fonseca Silva, Matheus Cecílio Fonseca, Thales Fernando Dias Pereira, Clebio Soares Nascimento, Eduardo Costa de Figueiredo, Keyller Bastos Borges
In this work, an efficient pipette tip based on molecularly imprinted polymers solid-phase extraction (PT-MIP-SPE) method was developed for carvedilol (CAR) analysis. This compound is available in clinical practice as a racemic mixture, in which (-)-(S)-CAR is a β- and α1-adrenergic antagonist, while (+)-(R)-CAR only acts as an α1-adrenergic antagonist. Enantioseparation of CAR presented satisfactory retention times (5.85 and 14.84min), acceptable theoretical plates (N=2048 and 2018) and good resolution (Rs=9...
August 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28802879/quadrulella-texcalense-sp-nov-from-a-mexican-desert-an-unexpected-new-environment-for-hyalospheniid-testate-amoebae
#13
Horacio Pérez-Juárez, Angélica Serrano-Vázquez, Anush Kosakyan, Edward A D Mitchell, Víctor M Rivera Aguilar, Daniel J G Lahr, Mayra M Hernández Moreno, Humberto Macías Cuellar, Luis E Eguiarte, Enrique Lara
Quadrulella (Amoebozoa, Arcellinida, Hyalospheniidae) is a genus of testate amoebae with unmistakable morphology, which secretes characteristic square plates to reinforce the test. They are mainly known from fens and freshwater habitats and have never been documented in deserts. We describe a new species, Quadrulella texcalense, from biological soil crusts in the intertropical desert of Tehuacán (state of Puebla, Mexico). Quadrulella texcalense occurred only at altitudes between 2140 and 2221m.a.s.l., together with the bryophyte genera Pseudocrossidium, Weissia, Bryum, Didymodon, Neohyophyla and Aloina...
July 17, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/28802426/comparison-between-antegrade-intramedullary-nailing-imn-open-reduction-plate-osteosynthesis-orpo-and-minimally-invasive-plate-osteosynthesis-mipo-in-treatment-of-humerus-diaphyseal-fractures
#14
Vidisha Sunil Kulkarni, Madhura Sujay Kulkarni, Govind Shivram Kulkarni, Vaibhav Goyal, Milind Govind Kulkarni
INTRODUCTION: The three currently used methods of treatment: namely open reduction plate osteosynthesis (ORPO), Minimally invasive plate osteosynthesis (MIPO), antegrade intramedullary nailing(IMN) are all reported as satisfactory procedures for treatment of humeral shaft fractures. However none of the published reports have a comparison of superiority of one procedure over the other. We evaluated the clinical, radiological and functional outcome of the three procedures. MATERIALS AND METHODS: We studied adult patients with humerus shaft fractures over a period of 2 years from May 2014 to May 2016 in a level 1 trauma center...
August 2017: Injury
https://www.readbyqxmd.com/read/28802424/can-antibiotic-impregnated-cement-nail-achieve-both-infection-control-and-bony-union-in-infected-diaphyseal-femoral-non-unions
#15
Chetan Pradhan, Atul Patil, Chetan Puram, Dheeraj Attarde, Parag Sancheti, Ashok Shyam
BACKGROUND: Infected non-union is complex and debilitating disorder affecting orthopaedic surgeon and patient in terms of cost and time. Many methods are described in the literature for treatment of infected non-union. Local high concentration of antibiotic and mechanical stability of antibiotic cement impregnated intramedullary nail (ACIIN) proves cost and time effective. Recently it was suggested that ACIIN can achieve both union and infection control in infected non-unions with bone gap less than 4cm...
August 2017: Injury
https://www.readbyqxmd.com/read/28802423/effectiveness-of-exchange-k-nail-and-augmented-plating-in-aseptic-femoral-diaphyseal-non-union
#16
K H Sancheti, Chetan Pradhan, Atul Patil, Chetan Puram, Parkalp Bharti, Parag Sancheti, Ashok Shyam
BACKGROUND: Femoral diaphyseal non-unions present difficult scenario to manage. There are multiple options but most of them still report varying incidences of failure. We combined the principles of augmented plating and exchange nailing and aim to study the effectiveness of this technique. METHOD: A retrospective study at a tertiary trauma centre was conducted. Seventy patients (60 men, 10 women), average age 40.7±15.27 years (range 18-81 years) with diaphyseal femoral fracture non-unions treated between July 2010 and January 2015 were reviewed...
August 2017: Injury
https://www.readbyqxmd.com/read/28802419/recalcitrant-aseptic-atrophic-non-union-of-the-shaft-of-the-humerus-after-failure-of-surgical-treatment-management-by-excision-of-non-union-bone-grafting-and-stabilization-by-lcp-in-different-modes
#17
Sudhir Babhulkar, Sushrut Babhulkar, Aditya Vasudev
Non-union of the humeral shaft is infrequently noticed after surgical fixation. Sixty eight patients whose osteosynthesis of humeral shaft had failed leading to non-union were identified over a duration of 10 years from (January 2006 to December 2015). Clinical and radiographical follow-up was available for 64 patients (4 patients were lost for follow-up), with a mean age of 58 years (range 25-78 years). All patients had aseptic atrophic non-union of either: proximal shaft (n=12), mid shaft (n=38), and lower shaft (n=14)...
August 2017: Injury
https://www.readbyqxmd.com/read/28802417/augmentation-plating-in-management-of-failed-femoral-nailing
#18
Rahul Verma, Pulak Sharma, Sanjiv Gaur
INTRODUCTION: Non-union following interlocking nailing is a troubling complication in treatment of shaft femur fractures. There is no clear consensus on the treatment of this problem. This research was undertaken to study the role of augmentation plating combined with exchange nailing in such cases. PATIENTS AND METHODS: A prospective clinical study was undertaken from January 2010 to December 2015. Patients with aseptic femoral shaft non-union, with or without implant failure following intramedullary nailing were included in the study...
August 2017: Injury
https://www.readbyqxmd.com/read/28802414/management-of-resistant-distal-femur-non-unions-with-allograft-strut-and-autografts-combined-with-osteosynthesis-in-a-series-of-22-patients
#19
Raja Bhaskara Kanakeshwar, Dheenadhayalan Jayaramaraju, Devendra Agraharam, Shanmuganathan Rajasekaran
INTRODUCTION AND PURPOSE: Challenges to the surgeon in managing cases of resistant non-union of the distal femur include poor bone stock, disuse osteopenia and joint contractures. Procedures varying from simple bone grafting to megaprosthesis revision have been described. We successfully managed such cases using our technique of combining cortical allograft struts to augment the defect in the femoral condyle coupled with autogenous iliac crest bone grafting and locking plate osteosynthesis...
August 2017: Injury
https://www.readbyqxmd.com/read/28802368/study-of-the-effects-of-hearing-on-static-and-dynamic-postural-function-in-children-using-cochlear-implants
#20
Malihah Mazaheryazdi, Abdollah Moossavi, Javad Sarrafzadah, Saeed Talebian, Shohreh Jalaie
OBJECTIVE: The present study aimed to evaluate the postural control perturbations by the center of pressure parameters in two main approaches, cochlear implant turned "on" and "off". METHODS: We included 25 children aged 8-10 years with unilateral cochlear implants and bilateral vestibular hypofunction deficit. To evaluate the postural function, each children was asked to stand on the force plate under 3 different conditions and cochlear implant turned "on" and "off": Condition (A) double stance from open eyes to closed eyes, Condition (B) double stance with open eyes engaging in the dual task and Condition (C) From double leg stance to one leg stance with open eyes for assessment of dynamic postural control...
September 2017: International Journal of Pediatric Otorhinolaryngology
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