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https://www.readbyqxmd.com/read/28087626/snail2-and-zeb2-repress-p-cadherin-to-define-embryonic-territories-in-the-chick-embryo
#1
Hervé Acloque, Oscar H Ocaña, Diana Abad, Claudio D Stern, M Angela Nieto
Snail and Zeb transcription factors induce epithelial to mesenchymal transition (EMT) in embryonic and adult tissues by direct repression of E-Cadherin transcription. The repression of E-Cadherin transcription by the EMT inducers Snail1 and Zeb2 plays a fundamental role in defining embryonic territories in the mouse, as E-Cadherin needs to be downregulated in the primitive streak and in the epiblast concomitant with the formation of mesendodermal precursors and the neural plate, respectively. Here we show that in the chick embryo, E-Cadherin is weakly expressed in the epiblast at pre-primitive streak stages where it is substituted by P-Cadherin We also show that Snail2 and Zeb2 repress P-Cadherin transcription in the primitive streak and the neural plate, respectively...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28087612/biofilm-formation-and-antimicrobial-resistance-genes-of-coagulase-negative-staphylococci-isolated-from-cows-with-mastitis-in-argentina
#2
Mariela E Srednik, Yannick D N Tremblay, Josée Labrie, Marie Archambault, Mario Jacques, Fernández Cirelli Alicia, Elida R Gentilini
Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008-2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation and Staphylococcus haemolyticus species formed the strongest biofilms...
January 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28087532/establishment-and-validation-of-rna-based-predictive-models-for-understanding-survival-of-vibrio-parahaemolyticus-in-oysters-stored-at-low-temperatures
#3
Chao Liao, Yong Zhao, Luxin Wang
: This study developed RNA-based predictive models describing the survival of Vibrio parahaemolyticus in Eastern oysters (Crassostrea virginica) during storage at 0, 4, and 10°C. Post-harvested oysters were inoculated with a cocktail of five V. parahaemolyticus strains and were then stored at 0, 4, and 10°C for 21 or 11 days. A real-time RT-PCR assay targeting expression of tlh gene was used to evaluate the number of surviving V. parahaemolyticus, which was then used to establish primary molecular models (MMs)...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28087466/-monkey-see-monkey-do-peers-behaviors-predict-preschoolers-physical-activity-and-dietary-intake-in-childcare-centers
#4
Stéphanie Ward, Mathieu Bélanger, Denise Donovan, Jonathan Boudreau, Hassan Vatanparast, Nazeem Muhajarine, Anne Leis, M Louise Humbert, Natalie Carrier
Preschoolers observe and imitate the behaviors of those who are similar to them. Therefore, peers may be role models for preschoolers' dietary intake and physical activity in childcare centers. This study examined whether peers' behaviors predict change in preschoolers' dietary intake and physical activity in childcare centers over 9months. A total of 238 preschoolers (3 to 5years old) from 23 childcare centers in two Canadian provinces provided data at the beginning (October 2013 and 2014) and the end (June 2014 and 2015) of a 9-month period for this longitudinal study...
January 10, 2017: Preventive Medicine
https://www.readbyqxmd.com/read/28087459/wnt-signaling-balances-specification-of-the-cardiac-and-pharyngeal-muscle-fields
#5
Amrita Mandal, Andrew Holowiecki, Yuntao Charlie Song, Joshua S Waxman
Canonical Wnt/β-catenin (Wnt) signaling plays multiple conserved roles during fate specification of cardiac progenitors in developing vertebrate embryos. Although lineage analysis in ascidians and mice has indicated there is a close relationship between the cardiac second heart field (SHF) and pharyngeal muscle (PM) progenitors, the signals underlying directional fate decisions of the cells within the cardio-pharyngeal muscle field in vertebrates are not yet understood. Here, we examined the temporal requirements of Wnt signaling in cardiac and PM development...
January 10, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/28087297/inhibition-of-streptococcal-biofilm-by-hydrogen-water
#6
Jinkyung Kim, Heon-Jin Lee, Su-Hyung Hong
OBJECTIVES: The accumulation of oral bacterial biofilm is the main etiological factor of oral diseases. Recently, electrolyzed hydrogen-rich water (H-water) has been shown to act as an effective antioxidant by reducing oxidative stress. In addition to this general health benefit, H-water has antibacterial activity for disease-associated oral bacteria. However, little is known about the effect of H-water on oral bacterial biofilm. The objective of this study was to confirm the effect of H-water on streptococcal biofilm formation...
January 10, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28087146/pcv13-vaccinated-children-still-carrying-pcv13-additional-serotypes-show-similar-carriage-density-to-a-control-group-of-pcv7-vaccinated-children
#7
Ron Dagan, Christine Juergens, James Trammel, Scott Patterson, David Greenberg, Noga Givon-Lavi, Nurith Porat, William C Gruber, Daniel A Scott
BACKGROUND: In addition to reducing vaccine-type nasopharyngeal carriage rates, pneumococcal conjugate vaccines (PCVs) may decrease carriage density in vaccinated individuals still carrying vaccine serotypes. However, reduction of carriage density has not been systematically studied. This study compared the effect of PCV13 versus PCV7 on carriage density of the serotypes in PCV13 that are not included in PCV7. METHODS: This randomized, double-blind study was conducted in southern Israel and included Jewish and Bedouin subjects...
January 10, 2017: Vaccine
https://www.readbyqxmd.com/read/28086905/reduction-of-eddy-current-losses-in-inductive-transmission-systems-with-ferrite-sheets
#8
Matthias Maaß, Andreas Griessner, Viktor Steixner, Clemens Zierhofer
BACKGROUND: Improvements in eddy current suppression are necessary to meet the demand for increasing miniaturization of inductively driven transmission systems in industrial and biomedical applications. The high magnetic permeability and the simultaneously low electrical conductivity of ferrite materials make them ideal candidates for shielding metallic surfaces. For systems like cochlear implants the transmission of data as well as energy over an inductive link is conducted within a well-defined parameter set...
January 5, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28086754/making-the-most-of-mealtimes-m3-protocol-of-a-multi-centre-cross-sectional-study-of-food-intake-and-its-determinants-in-older-adults-living-in-long-term-care-homes
#9
Heather H Keller, Natalie Carrier, Susan Slaughter, Christina Lengyel, Catriona M Steele, Lisa Duizer, K Steve Brown, Habib Chaudhury, Minn N Yoon, Alison M Duncan, Veronique M Boscart, George Heckman, Lita Villalon
BACKGROUND: Older adults living in long term care (LTC) homes are nutritionally vulnerable, often consuming insufficient energy, macro- and micronutrients to sustain their health and function. Multiple factors are proposed to influence food intake, yet our understanding of these diverse factors and their interactions are limited. The purpose of this paper is to fully describe the protocol used to examine determinants of food and fluid intake among older adults participating in the Making the Most of Mealtimes (M3) study...
January 13, 2017: BMC Geriatrics
https://www.readbyqxmd.com/read/28081317/hormone-therapy-in-hypospadias-surgery-a-survey-on-the-current-practice-in-turkey
#10
Gülnur Göllü Bahadır, Ergun Ergün, Onur Telli, Farid Khanmammadov, Ahmet Murat Çakmak
BACKGROUND/AIM: Nowadays surgical intervention is possible in smaller phalluses and younger children with hypospadias disease. Different hormone treatments with different doses, modalities, indications, and treatment times come along with some disputes. The aim of this study is to evaluate the management approaches in hypospadias surgery of surgeons in regards to hormone preparations. MATERIALS AND METHODS: Questionnaires were sent via e-mail to 110 actively working pediatric surgeons and urologists...
December 20, 2016: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28081267/single-nucleotide-variants-of-candidate-genes-in-aggrecan-metabolic-pathway-are-associated-with-lumbar-disc-degeneration-and-modic-changes
#11
Romain Shanil Perera, Poruwalage Harsha Dissanayake, Upul Senarath, Lalith Sirimevan Wijayaratne, Aranjan Lional Karunanayake, Vajira Harshadeva Weerabaddana Dissanayake
INTRODUCTION: Lumbar disc degeneration (LDD) is genetically determined and severity of LDD is associated with Modic changes. Aggrecan is a major proteoglycan in the intervertebral disc and end plate. Progressive reduction of aggrecan is a main feature of LDD and Modic changes. OBJECTIVES: The study investigated the associations of single nucleotide variants (SNVs) of candidate genes in the aggrecan metabolic pathway with the severity of LDD and Modic changes. In-silico functional analysis of significant SNVs was also assessed...
2017: PloS One
https://www.readbyqxmd.com/read/28081169/assessment-of-a-96-well-plate-assay-of-quantitative-drug-susceptibility-testing-for-mycobacterium-tuberculosis-complex-in-china
#12
Hui Xia, Yang Zheng, Bing Zhao, Susan van den Hof, Frank Cobelens, YanLin Zhao
OBJECTIVE: To evaluate the performance of the Sensitire MYCOTB MIC Plate (MYCOTB) which could measure the twelve anti-tuberculosis drugs susceptibility on one 96-wells plate. METHODS: A total of 140 MDR-TB strains and 60 non-MDR strains were sub-cultured and 193 strains were finally tested for drug resistance using MYCOTB and agar proportion method (APM) and another 7 strains failed of subculture. The drugs included ofloxacin (Ofx), moxifloxacin (Mfx), rifampin (RFP), amikacin (Am), rifabutin (Rfb), para-aminosalicylic acid (PAS), ethionamide (Eth), isoniazid (INH), kanamycin (Km), ethambutol (EMB), streptomycin (Sm), and cycloserine(Cs)...
2017: PloS One
https://www.readbyqxmd.com/read/28081009/influence-of-wall-thickness-and-diameter-on-arterial-shear-wave-elastography-a-phantom-and-finite-element-study
#13
Elira Maksuti, Fabiano Bini, Stefano Fiorentini, Giulia Blasi, Matthew Urban, Franco Marinozzi, Matilda Larsson
Quantitative, non-invasive and local measurements of arterial mechanical properties could be highly beneficial for early diagnosis of cardiovascular disease and follow up of treatment. Arterial Shear Wave Elastography (SWE) and wave velocity dispersion analysis have previously been applied to measure arterial stiffness. Arterial wall thickness (h) and inner diameter (D) vary with age and pathology and may influence the shear wave propagation. Nevertheless, the effect of arterial geometry in SWE has not yet been systematically investigated...
January 12, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28080022/single-wall-carbon-nanotubes-doping-in-lead-acid-batteries-a-new-horizon
#14
Anjan Banerjee, Baruch Ziv, Yuliya Shilina, Elena Levi, Shalom Luski, Doron Aurbach
The addition of single-wall carbon nanotubes (SWCNT) to lead-acid battery electrodes is the most efficient suppresser of uncontrolled sulfation processes. Due to the cost of SWCNT, we studied the optimization loading of SWCNT in lead-acid batteries electrodes. We optimized the SWCNT loading concentrations in both the positive and negative plates, separately. Loadings of 0.01% and 0.001% in the positive and negative active masses were studied, respectively. Two volts of lead-acid laboratory cells with sulfuric acid, containing silica gel-type electrolytes, were cycled in a 25% and 50% depth-of-discharge (DOD) cycling with a charging rate of C and 2C, respectively, and discharge rates of C/2 and C, respectively...
January 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28079842/increased-erythrocyte-aggregation-and-oxidative-stress-in-patients-with-idiopathic-interstitial-pneumonia
#15
Erhan Ugurlu, Emine Kilic-Toprak, Goksel Altinisik, Ozgen Kilic-Erkek, Betul Cengiz, Vural Kucukatay, Hande Senol, Ismail Hakki Akbudak, Yusuf Ekbic, Melek Bor-Kucukatay
BACKGROUND: Hemorheological properties are important determinants of tissue oxygenation. Although hemorheological alterations in various lung diseases have been well-defined, no information is available about the effects of idiopathic interstitial pneumonia (IIP) on hemorheological parameters. OBJECTIVES: The aim of this study was to investigate hemorheological parameters (erythrocyte deformability, aggregation, and plasma viscosity -PV) and associated oxidative stress indices in patients with IIP...
December 23, 2016: Sarcoidosis, Vasculitis, and Diffuse Lung Diseases: Official Journal of WASOG
https://www.readbyqxmd.com/read/28079741/management-of-pediatric-open-tibia-fractures-with-supracutaneous-locked-plates
#16
Veerappa N Radhakrishna, Vrisha Madhuri
We evaluated the novel application of supracutaneous locked plates in pediatric open tibia fractures. Pediatric open tibia fractures stabilized with a locked supracutaneous plate from January 2011 to December 2014 were reviewed. Twenty-eight children, mean age 8.9 years, with 29 open tibia fractures were included. Nine of these children who had metaphyseal or metadiaphyseal fractures did not require joint spanning. The mean follow-up duration was 13.5 months. The mean time to uneventful union was 11.46 weeks, with no unacceptable malunion...
January 10, 2017: Journal of Pediatric Orthopedics. Part B
https://www.readbyqxmd.com/read/28079730/biomechanical-comparison-of-cadaveric-and-commercially-available-synthetic-osteoporotic-bone-analogues-in-a-locked-plate-fracture-model-under-torsional-loading
#17
Edward H Becker, Hyunchul Kim, Michael Shorofsky, Adam H Hsieh, Jeffrey D Watson, Robert V O'Toole
OBJECTIVES: Biomechanical studies of osteoporotic bone have used synthetic models rather than cadaveric samples because of decreased variability, increased availability, and overall ease of use of synthetic models. We compared the torsional mechanical properties of cadaveric osteoporotic bone with those of currently available synthetic osteoporotic bone analogues. METHODS: We tested 12 osteoporotic cadaveric humeri and 6 specimens each of 6 types of synthetic analogues...
January 5, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28079599/steatohepatitis-like-changes-in-focal-nodular-hyperplasia-a-finding-to-distinguish-from-steatohepatitic-variant-of-hepatocellular-carcinoma
#18
Kemal Deniz, Roger K Moreira, Matthew M Yeh, Linda D Ferrell
Steatohepatitis-like change has not been described in focal nodular hyperplasia (FNH). Steatohepatitis-like change in FNH may show overlapping features with steatohepatitic variant of hepatocellular carcinoma (HCC). This problem can be compounded if seen in FNH with widened cell plates or hepatocyte rosettes, other features that can also be seen in HCC. This study examined steatotic FNHs for the frequency of steatohepatitis-like change, especially in the setting of FNH with rosettes and/or widened cell plates...
February 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28079120/in-situ-imaging-of-microstructure-formation-in-electronic-interconnections
#19
M A A Mohd Salleh, C M Gourlay, J W Xian, S A Belyakov, H Yasuda, S D McDonald, K Nogita
The development of microstructure during melting, reactive wetting and solidification of solder pastes on Cu-plated printed circuit boards has been studied by synchrotron radiography. Using Sn-3.0Ag-0.5Cu/Cu and Sn-0.7Cu/Cu as examples, we show that the interfacial Cu6Sn5 layer is present within 0.05 s of wetting, and explore the kinetics of flux void formation at the interface between the liquid and the Cu6Sn5 layer. Quantification of the nucleation locations and anisotropic growth kinetics of primary Cu6Sn5 crystals reveals a competition between the nucleation of Cu6Sn5 in the liquid versus growth of Cu6Sn5 from the existing Cu6Sn5 layer...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28078872/molecular-characterization-and-antibiotic-resistance-of-salmonella-serovars-isolated-in-the-apulia-region-of-italy
#20
R Mirgaldi, A Ballini, A M Dionisi, I Luzzi, G Dipalma, F Inchingolo, D De Vito
During the period January 2013-December 2015, 175 cases of human salmonellosis were reported in the Apulia Region of Italy. The aim of this study was to characterize salmonella strains from the standpoints of serovars prevalence, antimicrobial resistance and clonal origin. The serological typing was performed by agglutination against antisera followed by a multiplex polymerase chain reaction (m-PCR). The obtained results were analyzed following the Kauffmann-White scheme. Susceptibility to antimicrobial agents was tested using the disk diffusion method on Muller-Hinton agar plates...
October 2016: Journal of Biological Regulators and Homeostatic Agents
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