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Delgerdalai Khashbat Md, Takashi Abe Md, Mungunbagana Ganbold Md, Seiji Iwamoto Md, Naoto Uyama Md, Saho Irahara Md, Youichi Otomi Md PhD, Masafumi Harada Md PhD, Teruyoshi Kageji Md PhD, Shinji Nagahiro Md PhD
PURPOSE: Arterial spin labeling (ASL) is an alternative method to Dynamic susceptibility contrast (DSC) perfusion MRI for brain tumors. However, ASL cerebral blood flow (CBF) can be easily affected by transit time. DSC MRI derived time to maximum of the residue function (Tmax) is possible to assess the transit time on ASL. METHODS: Thirty patients with brain tumors were studied using ASL and DSC MRI. The relative cerebral blood flow (rCBF), relative cerebral blood volume (rCBV), Tmax, and mean transit time (MTT) were obtained from DSC MRI...
2016: Journal of Medical Investigation: JMI
Harshini Togaru, Radhika Muppa, NCh Srinivas, K Naveen, Veerakishore K Reddy, V C Rebecca
AIM: The aim of this study is to evaluate and compare Biodentine and mineral trioxide aggregate (MTA) as pulpotomy agents by clinical and radiological assessments in primary teeth. MATERIALS AND METHODS: In this study, 90 decayed primary molars indicated for pulpotomy were chosen and are divided into two groups. Soft enamel and dentinal caries were removed using spoon excavator. Access opening was done using highspeed cool water handpiece. Normal saline was used to irrigate pulp chamber, later saline moistened cotton pellet was used to obtain hemostasis in both groups...
2016: Journal of Contemporary Dental Practice
Otomi Cho, Tomoe Ichikawa, Sanae Kurakado, Masako Takashima, Ri-Ichiroh Manabe, Moriya Ohkuma, Takashi Sugita
Here, we report the draft genome sequence of Trichosporon domesticum JCM 9580, isolated from the house of a patient with summer-type hypersensitivity pneumonitis (SHP) in Japan. This genomic information will help elucidate the mechanisms of the development of SHP.
2016: Genome Announcements
Yolanda Aguilera, Luis Mojica, Miguel Rebollo-Hernanz, Mark Berhow, Elvira González de Mejía, María A Martín-Cabrejas
Anthocyanin-rich powders and aqueous extracts, with high antioxidant activities, were obtained from black bean seed coats and applied to colour a sport beverage. Idaho and Otomi bean coats were extracted in water-citric acid 2% (1/50, w/v), stirring for 4h at 40°C. Anthocyanins from Idaho and Otomi extracts (1.83mg and 1.02mg C3G/g, respectively) were applied to a commercially available sport beverage, with and without 2% β-cyclodextrin (βCD) under light and darkness conditions for 10days, and stored at 4°C and 25°C for 6weeks...
December 1, 2016: Food Chemistry
Otomi Cho, Takashi Sugita
As DNA sequences of the intergenic spacer (IGS) region in the rRNA gene show remarkable intraspecies diversity compared with the small subunit, large subunit, and internal transcribed spacer region, the IGS region has been used as an epidemiological tool in studies on Malassezia globosa and M. restricta, which are responsible for the exacerbation of atopic dermatitis (AD) and seborrheic dermatitis (SD). However, the IGS regions of M. sympodialis and M. dermatis obtained from the skin of patients with AD and SD, as well as healthy subjects, lacked sequence diversity...
July 1, 2016: Mycopathologia
Akiomi Tanaka, Otomi Cho, Chie Saito, Mami Saito, Ryoji Tsuboi, Takashi Sugita
Seborrheic dermatitis (SD) is a chronic inflammatory dermatologic condition in which erythema and itching develop on areas of the body with sebaceous glands, such as the scalp, face and chest. The inflammation is evoked directly by oleic acid, which is hydrolyzed from sebum by lipases secreted by skin microorganisms. Although the skin fungal genus, Malassezia, is thought to be the causative agent of SD, analysis of the bacterial microbiota of skin samples of patients with SD is necessary to clarify any association with Malassezia because the skin microbiota comprises diverse bacterial and fungal genera...
August 2016: Microbiology and Immunology
Max Maizels, Dennis Liu, Edward Ming-Luan Gong, William E Kaplan, Earl Y Cheng
No abstract text is available yet for this article.
June 2016: Journal of Pediatric Urology
Yoichi Otomi, Takayoshi Shinya, Hideki Otsuka, Kaori Terazawa, Saho Irahara, Saki Nagase, Ayaka Takahashi, Michiko Kubo, Masafumi Harada
OBJECTIVE: The purpose of the study was to evaluate the hypothesis that patients having a vasovagal reaction (VVR) after blood vessel puncture show increased FDG accumulation in bilateral adrenal glands. METHODS: Over the past 8 years, 26 patients experienced a VVR after blood vessel puncture following intra-venous injection of FDG at our institution. Of the 26 patients, 16 underwent multiple-occasion FDG-PET/CT scans while suffering a VVR at only one examination...
August 2016: Annals of Nuclear Medicine
Tomoe Ichikawa, Otomi Cho, Takashi Sugita, Yoshio Ishibashi, Reiko Ikeda
Cryptococcus gattii and C. neoformans are pathogenic yeasts that cause meningoencephalitis. C. gattii has four molecular types: VGI, VGII, VGIII, and VGIV. Furthermore, three genotypes have been reported for VGII, and a high pathogenicity of the VGIIa genotype has been proposed. The VGIIa strain has been isolated from a patient in Japan, but little is known about the characteristics of the polysaccharides in this strain. In this study we examined the induction of interleukin-8(IL-8)transcriptional activation and compared the nuclear magnetic resonance(NMR)spectra of extracellular polymeric substances(EPSs), mainly polysaccharides, from the VGIIa, VGIIb, and VGIIc genotypes...
2016: Medical Mycology Journal
Takashi Sugita, Takashi Yamazaki, Koichi Makimura, Otomi Cho, Shin Yamada, Hiroshi Ohshima, Chiaki Mukai
The International Space Station (ISS) is a huge manned construct located approximately 400 km above the earth and is inhabited by astronauts performing space experiments. Because the station is within a closed microgravity environment, the astronauts are subject to consistent stress. This study analyzed the temporal changes in the skin fungal microbiota of 10 astronauts using pyrosequencing and quantitative PCR assay before, during, and after their stay in the ISS. Lipophilic skin fungi, Malassezia predominated most samples regardless of the collection period, body site (cheek or chest), or subject...
March 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Otomi Cho, Manami Matsukura, Takashi Sugita
The fungus Trichosporon asahii is distributed widely in the environment and is frequently isolated from clinical specimens. It can cause life-threatening disseminated infections, including breakthrough infection after the administration of candin antifungal drugs. However, the definitive infection route is still unclear. In this study, 43 T. asahii genotypes were detected in 72 fecal samples from healthy subjects. The rRNA genotypes of the species were determined using a culture-independent method. The genotypes of T...
October 2015: International Journal of Infectious Diseases: IJID
A Al Varshavskiy, A A Varshavskiy
Cellobiohydrolase activity (CBHA) of endosymbionts in the digestive tract of two African rodent species differing in its morphology and in feeding specialization--Arvicanthis niloticus and Otomys helleri--has been studied as a characteristic of their physiological and ecological adaptation to phytophagy A statistically significant correlation of CBHA with obesity has been revealed in A. niloticus, with the CBHA level being significantly higher in corpus ceci than in ampulla ceci. The possible morphophysiological and ecological significance of the observed features of symbiotic digestion are discussed...
May 2015: Izvestiia Akademii Nauk. Seriia Biologicheskaia
Takashi Sugita, Takashi Yamazaki, Shin Yamada, Hajime Takeoka, Otomi Cho, Takafumi Tanaka, Giichiro Ohno, Kentaro Watanabe, Koichi Makimura, Hiroshi Ohshima, Noriaki Ishioka, Chiaki Mukai
The International Space Station (ISS) is located approximately 400 km above the Earth. Astronauts staying at the ISS are under microgravity and are thus unable to bathe or shower; instead, they wash their bodies using wet tissues. For astronauts, skin hygiene management is important to maintain the quality of life during long-term stays on the ISS. In Antarctica, members of a Japanese geological investigation team negotiate their way over land using snowmobiles. During their 3-month stay, they are subject to a "pseudo-space" environment similar to that experienced by ISS astronauts, including the inability to bathe or shower...
September 2015: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Mungunkhuyag Majigsuren, Takashi Abe, Teruyoshi Kageji, Kenji Matsuzaki, Mayumi Takeuchi, Seiji Iwamoto, Yoichi Otomi, Naoto Uyama, Shinji Nagahiro, Masafumi Harada
PURPOSE: T1-Cube (GE HealthCare) is a relatively new 3-dimensional (3D) fast spin-echo (FSE)-based magnetic resonance (MR) imaging sequence that uses a variable flip angle to acquire gap-free volume scans. We compared the gadolinium enhancement characteristics of a heterogeneous population of brain tumors imaged by T1-Cube and then 3D fast spoiled gradient recall acquisition in steady state (3D FSPGR) 3-tesla MR imaging to identify the superior modality for specific diagnostic purposes...
2016: Magnetic Resonance in Medical Sciences: MRMS
Takashi Abe, Yoshifumi Mizobuchi, Wataru Sako, Saho Irahara, Yoichi Otomi, Yuki Obama, Kohei Nakajima, Delgerdalai Khashbat, Mungunkhuyag Majigsuren, Teruyoshi Kageji, Shinji Nagahiro, Masafumi Harada
PURPOSE: In the imaging of intra-axial brain tumors, we sometimes found areas of high signal intensity around the enhanced tumor lesions on arterial spin labeling (ASL) magnetic resonance (MR) imaging. We undertook this study to investigate the relationship between high signal intensity on ASL imaging outside the area of contrast enhancement (CE) and histological diagnosis of intra-axial brain tumors. METHODS: We examined images from 28 consecutive patients with intra-axial brain tumors who underwent ASL and CE MR imaging-three with low grade glioma (LGG), 13 with high grade glioma (HGG), six with metastasis, and six with primary central nervous system lymphoma (PCNSL)-and divided imaging findings into an "ASL dominant" group when hyperintensity on ASL was found outside the CE area and a "CE dominant" group when hyperintensity on ASL was not found outside the area of enhancement...
2015: Magnetic Resonance in Medical Sciences: MRMS
Masako Takashima, Otomi Cho, Takashi Sugita
No abstract text is available yet for this article.
2015: Medical Mycology Journal
Ana Itzel Juárez-Martín, Blanca Zoila González-Sobrino, Ángel Eduardo Camarena Olvera, Ramcés Falfán-Valencia
From a historical and genetic point of view, the Otomi of the Mezquital Valley are a frontier people that have played an important role in the population dynamics of the Mexican Central Plateau. Due to the antiquity of their presence in the area, the Otomi may be bearers of ancient genetic variability, shared mainly today with other groups belonging to the Otomanguean linguistic family and with the Nahua. In this study we analyzed the HLA class II allele frequencies reported in Mexican indigenous populations, in order to provide an intraregional-level historical perspective of the genetic relationships between the Otomi of the Mezquital Valley and indigenous populations from other regions of Mexico...
2014: Human Biology
Takashi Abe, Yoshifumi Mizobuchi, Kohei Nakajima, Yoichi Otomi, Saho Irahara, Yuki Obama, Mungunkhuyag Majigsuren, Delgerdalai Khashbat, Teruyoshi Kageji, Shinji Nagahiro, Masafumi Harada
This study sought to determine the diagnostic utility of perfusion parameters derived from dynamic contrast-enhanced (DCE) perfusion MRI with a short acquisition time (approximately 3.5 min) in patients with glioma, brain metastasis, and primary CNS lymphoma (PCNSL). Twenty-six patients with 29 lesions (4 low-grade glioma, 13 high-grade glioma, 7 metastasis, and 5 PCNSL) underwent DCE-MRI in a 3 T scanner. A ROI was placed on the hotspot of each tumor in maps for volume transfer contrast K (trans) , extravascular extracellular volume V e , and fractional plasma volume V p ...
2015: SpringerPlus
Akemi Takemoto, Otomi Cho, Yuka Morohoshi, Takashi Sugita, Masahiko Muto
The skin surface is colonized by a wide variety of fungi and bacteria. While many of these organisms, including Malassezia, Candida, Streptococcus and Staphylococcus species, are associated with provocation and/or exacerbation of psoriasis, a detailed analysis of the cutaneous fungal microbiome in psoriatic patients has yet to be performed. To identify the disease-specific fungal microbiota on psoriatic scale samples, fungal rRNA gene sequences from 12 psoriatic patients and 12 healthy controls were analyzed by pyrosequencing...
February 2015: Journal of Dermatology
Yimin Chen, Liansheng Qiu, Jinmei Qiu
OBJECTIVE: This study was to investigate the clinical symptoms, characterization of imaging technique and the effect of endonasal endoscopic surgery. METHOD: Following 38 cases of isolated sphenoid sinus disease patients, among whom 35 cases treated with nasal sinus CT (including 5 cases of MRI at the same time), 3 cases undergoing sinus MRI, 1 cases going through the CT cisternography. All patients underwent endoscopic sphenoid otomy, of which 33 cases underwent nasal olfactory cleft approach, 5 cases anterior ethmoid sinus and posterior ethmoid sinus approach (Messerklinger technology)...
June 2014: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
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