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https://www.readbyqxmd.com/read/29439246/cyclic-mechanical-stretch-induced-smooth-muscle-cell-changes-in-cerebral-aneurysm-progress-by-reducing-collagen-type-iv-and-collagen-type-vi-levels
#1
Peixi Liu, Yaying Song, Yingjie Zhou, Yingjun Liu, Tianming Qiu, Qingzhu An, Jianping Song, Peiliang Li, Yuan Shi, Sichen Li, Kai Quan, Guo-Yuan Yang, Wei Zhu
BACKGROUND/AIMS: Cerebral aneurysm growth is characterized by continuous structural weakness of local smooth muscle cells, though the mechanism is unclear. In this study, we examine protein changes in cerebral aneurysm and human brain vascular smooth muscle cells after cyclic mechanical stretch. We further explore the relationship between the smooth muscle cell changes and reductions in the levels of collagen types IV and VI. METHODS: Saccular cerebral aneurysms (n=10) were collected, and temporal artery samples were used as controls...
February 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29426953/cerebral-ischemia-induces-the-aggregation-of-proteins-linked-to-neurodegenerative-diseases
#2
Anja Kahl, Ismary Blanco, Katherine Jackman, Juhi Baskar, Harihar Milaganur Mohan, Reunet Rodney-Sandy, Sheng Zhang, Costantino Iadecola, Karin Hochrainer
Protein aggregation critically affects cell viability in neurodegenerative diseases, but whether this also occurs in ischemic brain injury remains elusive. Prior studies report the post-ischemic aggregation of ubiquitin, small ubiquitin-related modifier (SUMO) and ribosomes, however whether other proteins are also affected is unknown. Here we employed a proteomic approach to identify the insoluble, aggregated proteome after cerebral ischemia. Mice underwent transient middle cerebral artery occlusion or sham-surgery...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29416745/itraq-analysis-of-a-mouse-acute-myocardial-infarction-model-reveals-that-vitamin-d-binding-protein-promotes-cardiomyocyte-apoptosis-after-hypoxia
#3
Yun Wu, Fen Liu, Xiang Ma, Dilare Adi, Ming-Tao Gai, Xiang Jin, Yi-Ning Yang, Ying Huang, Xiang Xie, Xiao-Mei Li, Zhen-Yan Fu, Bang-Dang Chen, Yi-Tong Ma
The proteome profile changes after acute myocardial infarction (AMI) and the roles played by important protein species remain poorly understood. Here, we constructed a mouse AMI model by ligating the left coronary artery of male C57B/6J mice to investigate the molecular changes after AMI on the protein level. Total proteins of the left ventricle were extracted and quantitatively analyzed by isobaric tags using relative and absolute quantitation (iTRAQ) technologies. The transcript and protein levels of important genes were further validated using quantitative polymerase chain reaction and western blot...
January 5, 2018: Oncotarget
https://www.readbyqxmd.com/read/29399028/quercetin-attenuates-the-injury-induced-reduction-of-%C3%AE-enolase-expression-in-a-middle-cerebral-artery-occlusion-animal-model
#4
Seong-Jun Jeon, Myeong-Ok Kim, Fawad Ali-Shah, Phil-Ok Koh
Quercetin, a natural flavonoid, copiously exists in vegetable, fruits and tea. Quercetin is beneficial to neurodegenerative disorders via its strong anti-oxidant and anti-inflammatory activities. γ-Enolase is one of the enzymes of glycolytic pathway and is predominantly expressed in neuronal cells. The aim of the present study is to verify whether quercetin modulates the expression of γ-enolase in brain ischemic injury. Adult Sprague-Dawley male rats were subjected to middle cerebral artery occlusion (MCAO) and quercetin (50 mg/kg) or vehicle was administered by intraperitoneal injection at 1 h before MCAO onset...
December 2017: Laboratory Animal Research
https://www.readbyqxmd.com/read/29395098/distinctive-molecular-signature-and-activated-signaling-pathways-in-aortic-smooth-muscle-cells-of-patients-with-myocardial-infarction
#5
Thidathip Wongsurawat, Chin Cheng Woo, Antonis Giannakakis, Xiao Yun Lin, Esther Sok Hwee Cheow, Chuen Neng Lee, Mark Richards, Siu Kwan Sze, Intawat Nookaew, Vladimir A Kuznetsov, Vitaly Sorokin
BACKGROUND AND AIMS: We aim to identify significant transcriptome alterations of vascular smooth muscle cells (VSMCs) in the aortic wall of myocardial infarction (MI) patients. Providing a robust transcriptomic signature, we aim to highlight the most likely aberrant pathway(s) in MI VSMCs. METHODS AND RESULTS: Laser-captured microdissection (LCM) was used to obtain VSMCs from aortic wall tissues harvested during coronary artery bypass surgery. Microarray gene analysis was applied to analyse VSMCs from 17 MI and 19 non-MI patients...
January 29, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29378352/atherothrombosis-and-thromboembolism-position-paper-from-the-second-maastricht-consensus-conference-on-thrombosis
#6
H M H Spronk, T Padro, J E Siland, J H Prochaska, J Winters, A C van der Wal, J J Posthuma, G Lowe, E d'Alessandro, P Wenzel, D M Coenen, P H Reitsma, W Ruf, R H van Gorp, R R Koenen, T Vajen, N A Alshaikh, A S Wolberg, F L Macrae, N Asquith, J Heemskerk, A Heinzmann, M Moorlag, N Mackman, P van der Meijden, J C M Meijers, M Heestermans, T Renné, S Dólleman, W Chayouâ, R A S Ariëns, C C Baaten, M Nagy, A Kuliopulos, J J Posma, P Harrison, M J Vries, H J G M Crijns, E A M P Dudink, H R Buller, Y M C Henskens, A Själander, S Zwaveling, O Erküner, J W Eikelboom, A Gulpen, F E C M Peeters, J Douxfils, R H Olie, T Baglin, A Leader, U Schotten, B Scaf, H M M van Beusekom, L O Mosnier, L van der Vorm, P Declerck, M Visser, D W J Dippel, V J Strijbis, K Pertiwi, A J Ten Cate-Hoek, H Ten Cate
Atherothrombosis is a leading cause of cardiovascular mortality and long-term morbidity. Platelets and coagulation proteases, interacting with circulating cells and in different vascular beds, modify several complex pathologies including atherosclerosis. In the second Maastricht Consensus Conference on Thrombosis, this theme was addressed by diverse scientists from bench to bedside. All presentations were discussed with audience members and the results of these discussions were incorporated in the final document that presents a state-of-the-art reflection of expert opinions and consensus recommendations regarding the following five topics: 1...
February 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29377410/inhibition-of-aldehyde-dehydrogenase-activity-expands-multipotent-myeloid-progenitor-cells-with-vascular-regenerative-function
#7
Tyler T Cooper, Stephen E Sherman, Miljan Kuljanin, Gillian I Bell, Gilles A Lajoie, David A Hess
Blood-derived progenitor cell transplantation holds potential for the treatment of severe vascular diseases. Human umbilical cord blood (UCB)-derived hematopoietic progenitor cells purified using high aldehyde dehydrogenase (ALDH hi ) activity demonstrate pro-angiogenic functions following intramuscular (i.m.) transplantation into immunodeficient mice with hind-limb ischemia. Unfortunately, UCB ALDH hi cells are rare and prolonged ex vivo expansion leads to loss of high ALDH-activity and diminished vascular regenerative function...
January 27, 2018: Stem Cells
https://www.readbyqxmd.com/read/29365926/proteomics-discovery-of-biomarkers-in-statin-treated-patients-with-advanced-coronary-artery-disease
#8
Aspasia Tzani, Ilias Doulamis, Panagiotis Konstantopoulos, Asier Antoranz, Vaia Pliaka, Aggeliki Minia, Anastasios Gkogkos, George Samanidis, Theodore Sakellaropoulos, Leonidas Alexopoulos, Despina Perrea, Konstantinos Perreas
No abstract text is available yet for this article.
August 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29336364/mechanism-of-chronic-stress-induced-reduced-atherosclerotic-medial-area-and-increased-plaque-instability-in-rabbit-models-of-chronic-stress
#9
Ze-Mou Yu, Xiao-Tao Deng, Ruo-Mei Qi, Lu-Yan Xiao, Chong-Qing Yang, Tao Gong
BACKGROUND: Chronic stress contributes to increased risks of atherosclerotic diseases including heart disease, stroke, and transient ischemic attack. However, its underline mechanisms are poorly understood. This study aimed to elucidate the mechanism via which chronic stress exerts its effect on atherosclerosis (AS). METHODS: Fifty male New Zealand white rabbits were used. Aortic balloon-injury model was applied. Both social stress and physical stress methods were adopted to establish chronic stress models...
January 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29284736/carotid-atheroma-from-men-has-significantly-higher-levels-of-inflammation-and-iron-metabolism-enabled-by-macrophages
#10
Xi-Ming Yuan, Liam J Ward, Claes Forssell, Nabeel Siraj, Wei Li
BACKGROUND AND PURPOSE: Men differ from women in the manifestation of atherosclerosis and iron metabolism. Intraplaque hemorrhage and hemoglobin (Hb) catabolism by macrophages are associated with atherosclerotic lesion instability. The study aims were to investigate sex differences in (1) lesion severity in relation to blood Hb, (2) iron homeostasis in human carotid plaques, and (3) macrophage polarization within atheroma. METHODS: The carotid artery samples from 39 men and 23 women were immunostained with cell markers for macrophages, smooth muscle cells, ferritin, and TfR1 (transferrin receptor 1), which were further analyzed according to sex in relation to iron, Hb, and lipids in circulation...
December 28, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/29258604/water-t2-as-an-early-global-and-practical-biomarker-for-metabolic-syndrome-an-observational-cross-sectional-study
#11
Michelle D Robinson, Ina Mishra, Sneha Deodhar, Vipulkumar Patel, Katrina V Gordon, Raul Vintimilla, Kim Brown, Leigh Johnson, Sid O'Bryant, David P Cistola
BACKGROUND: Metabolic syndrome (MetS) is a highly prevalent condition that identifies individuals at risk for type 2 diabetes mellitus and atherosclerotic cardiovascular disease. Prevention of these diseases relies on early detection and intervention in order to preserve pancreatic β-cells and arterial wall integrity. Yet, the clinical criteria for MetS are insensitive to the early-stage insulin resistance, inflammation, cholesterol and clotting factor abnormalities that characterize the progression toward type 2 diabetes and atherosclerosis...
December 19, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29258572/effects-of-four-antiplatelet-statin-combined-strategies-on-immune-and-inflammatory-responses-in-patients-with-acute-myocardial-infarction-undergoing-pharmacoinvasive-strategy-design-and-rationale-of-the-b-and-t-types-of-lymphocytes-evaluation-in-acute-myocardial
#12
Francisco A H Fonseca, Maria Cristina Izar, Ieda M L Maugeri, Otavio Berwanger, Lucas P Damiani, Ibraim M Pinto, Gilberto Szarf, Carolina N França, Henrique T Bianco, Flavio T Moreira, Adriano Caixeta, Claudia M R Alves, Aline Soriano Lopes, Aline Klassen, Marina F M Tavares, Henrique A Fonseca, Antonio C C Carvalho
BACKGROUND: Early reperfusion of the occluded coronary artery during acute myocardial infarction is considered crucial for reduction of infarcted mass and recovery of ventricular function. Effective microcirculation and the balance between protective and harmful lymphocytes may have roles in reperfusion injury and may affect final ventricular remodeling. METHODS/DESIGN: BATTLE-AMI is an open-label, randomized trial comparing the effects of four therapeutic strategies (rosuvastatin/ticagrelor, rosuvastatin/clopidogrel, simvastatin plus ezetimibe/ticagrelor, or simvastatin plus ezetimibe/clopidogrel) on infarcted mass and left ventricular ejection fraction (LVEF) (blinded endpoints) in patients with ST-segment elevation myocardial infarction submitted to fibrinolytic therapy before coronary angiogram (pharmacoinvasive strategy)...
December 19, 2017: Trials
https://www.readbyqxmd.com/read/29158353/blood-pressure-control-by-a-secreted-fgfbp1-fibroblast-growth-factor-binding-protein
#13
Elena Tassi, En Yin Lai, Lingli Li, Glenn Solis, Yifan Chen, William E Kietzman, Patricio E Ray, Anna T Riegel, William J Welch, Christopher S Wilcox, Anton Wellstein
Fibroblast growth factors (FGFs) participate in organ development and tissue maintenance, as well as the control of vascular function. The paracrine-acting FGFs are stored in the extracellular matrix, and their release is controlled by a secreted FGF-binding protein (FGF-BP, FGFBP1, and BP1) that modulates FGF receptor signaling. A genetic polymorphism in the human FGFBP1 gene was associated with higher gene expression and an increased risk of familial hypertension. Here, we report on the effects of inducible BP1 expression in a transgenic mouse model...
November 20, 2017: Hypertension
https://www.readbyqxmd.com/read/29154769/extracellular-lipids-accumulate-in-human-carotid-arteries-as-distinct-three-dimensional-structures-and-have-proinflammatory-properties
#14
Satu Lehti, Su D Nguyen, Ilya Belevich, Helena Vihinen, Hanna M Heikkilä, Rabah Soliymani, Reijo Käkelä, Jani Saksi, Matti Jauhiainen, Gregory A Grabowski, Outi Kummu, Sohvi Hörkkö, Marc Baumann, Perttu J Lindsberg, Eija Jokitalo, Petri T Kovanen, Katariina Öörni
Lipid accumulation is a key characteristic of advancing atherosclerotic lesions. Here we analyzed the ultrastructure of the accumulated lipids in endarterectomized human carotid atherosclerotic plaques using three-dimensional (3D) electron microscopy, a method never used in this context before. 3D-electron microscopy revealed intracellular lipid droplets and extracellular lipoprotein particles. The majority of the particles were aggregated and some connected to needle-shaped or sheet-like cholesterol crystals...
November 14, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/29130072/prediction-of-chronic-kidney-disease-stage-3-by-ckd273-a-urinary-proteomic-biomarker
#15
Claudia Pontillo, Zhen-Yu Zhang, Joost P Schanstra, Lotte Jacobs, Petra Zürbig, Lutgarde Thijs, Adela Ramírez-Torres, Hiddo J L Heerspink, Morten Lindhardt, Ronald Klein, Trevor Orchard, Massimo Porta, Rudolf W Bilous, Nishi Charturvedi, Peter Rossing, Antonia Vlahou, Eva Schepers, Griet Glorieux, William Mullen, Christian Delles, Peter Verhamme, Raymond Vanholder, Jan A Staessen, Harald Mischak, Joachim Jankowski
Introduction: CKD273 is a urinary biomarker, which in advanced chronic kidney disease predicts further deterioration. We investigated whether CKD273 can also predict a decline of estimated glomerular filtration rate (eGFR) to <60 ml/min per 1.73 m2. Methods: In analyses of 2087 individuals from 6 cohorts (46.4% women; 73.5% with diabetes; mean age, 46.1 years; eGFR ≥ 60 ml/min per 1.73 m2, 100%; urinary albumin excretion rate [UAE] ≥20 μg/min, 6.2%), we accounted for cohort, sex, age, mean arterial pressure, diabetes, and eGFR at baseline and expressed associations per 1-SD increment in urinary biomarkers...
November 2017: KI Reports
https://www.readbyqxmd.com/read/29129081/identification-of-mature-atherosclerotic-plaque-proteome-signatures-using-data-independent-acquisition-mass-spectrometry
#16
Nicole Hansmeier, Josef Buttigieg, Pankaj Kumar, Shaneen Pelle, Kyoo Yoon Choi, David Kopriva, Tzu-Chiao Chao
Atherosclerosis is a chronic inflammatory disease with complex pathobiology and one of the most common causes of cardiovascular events. The process is characterized by complex vascular remodeling processes that require the actions of numerous proteins. The composition of atherosclerotic plaque is increasingly recognized as a major factor governing the occurrence of cardiovascular or neurological symptoms. In order to gain deeper insights in the composition of atherosclerotic plaques, we created quantitative proteome profiles of advanced plaque tissues of six male patients undergoing carotid endarterectomy for stroke prevention...
November 11, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29095649/loss-of-smad3-promotes-vascular-remodeling-in-pulmonary-arterial-hypertension-via-mrtf-disinhibition
#17
Diana Zabini, Elise Granton, Yijie Hu, Maria Zena Miranda, Ulrike Weichelt, Sandra Breuils Bonnet, Sébastien Bonnet, Nicholas W Morrell, Kim A Connelly, Steeve Provencher, Bahil Ghanim, Walter Klepetko, Andrea Olschewski, Andras Kapus, Wolfgang M Kuebler
INTRODUCTION: Vascular remodeling in pulmonary arterial hypertension (PAH) results from smooth muscle cell hypertrophy and proliferation of vascular cells. Loss of bone morphogenetic protein receptor 2 (BMPR-II) signaling and increased signaling via transforming growth factor β (TGF-β) and its downstream mediators SMAD2/3 has been proposed to drive lung vascular remodeling; yet, proteomic analyses indicate a loss of SMAD3 in PAH. OBJECTIVE: We proposed that SMAD3 may be dysregulated in PAH, and that loss of SMAD3 may present a master switch in the disease process by disinhibiting its interaction partner myocardin-related transcription factor (MRTF) which drives muscle protein expression...
November 2, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29094209/comparison-of-the-protective-effects-of-direct-ischemic-preconditioning-and-remote-ischemic-preconditioning-in-a-rabbit-model-of-transient-spinal-cord-ischemia
#18
Takehiko Fukui, Kazuyoshi Ishida, Yoichi Mizukami, Kiyotaka Shiramoto, Hidenori Harada, Atsuo Yamashita, Satoshi Yamashita, Mishiya Matsumoto
INTRODUCTION: This study aimed to determine the relative potency of direct ischemic preconditioning (DIPC) and remote ischemic preconditioning (RIPC) for protection against ischemic spinal cord injury in rabbits and to explore the mechanisms involved. METHODS: In experiment 1, we compared the neurological and histopathological outcomes of DIPC, kidney RIPC, and limb RIPC. The DIPC and kidney RIPC groups received two cycles of 5-min occlusion/15-min reperfusion of the abdominal aorta and left renal artery, respectively...
November 1, 2017: Journal of Anesthesia
https://www.readbyqxmd.com/read/29068691/myocardial-injury-is-distinguished-from-stable-angina-by-a-set-of-candidate-plasma-biomarkers-identified-using-itraq-mrm-based-approach
#19
Esther Sok Hwee Cheow, Woo Chin Cheng, Terence Yap, Bamaprasad Dutta, Chuen Neng Lee, Dominique P V de Kleijn, Vitaly Sorokin, Siu Kwan Sze
The lack of precise biomarkers that identify patients at risk for myocardial injury and stable angina delays administration of optimal therapy. Hence, the search for noninvasive biomarkers that could accurately stratify patients with impending heart attack, from patients with stable coronary artery disease (CAD), is urgently needed in the clinic. Herein, we performed comparative quantitative proteomics on whole plasma sampled from patients with stable angina (NMI), acute myocardial infarction (MI), and healthy control subjects (Ctrl)...
November 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29061397/diabetes-aggravates-decreases-in-hippocalcin-and-parvalbumin-expression-in-focal-cerebral-ischemia
#20
Dong-Ju Park, Phil-Ok Koh
Hyperglycemia is a major risk factor for stroke and increases brain damage during ischemic stroke. Hyperglycemia increases the intracellular calcium concentration after ischemic injury, thereby triggering neuronal cell death. Calcium binding proteins, including hippocalcin and parvalbumin, are critical regulators of intracellular calcium levels. This study aimed to investigate whether hyperglycemic conditions affect hippocalcin and parvalbumin expression during ischemic brain injury. Male adult rats were treated intraperitoneally with streptozotocin (40mg/kg) to induce hyperglycemia...
October 20, 2017: Neuroscience Letters
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