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https://www.readbyqxmd.com/read/27886187/plasma-proteomic-study-in-pulmonary-arterial-hypertension-associated-with-congenital-heart-diseases
#1
Xi Zhang, Hai-Tao Hou, Jun Wang, Xiao-Cheng Liu, Qin Yang, Guo-Wei He
Pulmonary arterial hypertension associated with congenital heart disease (CHD-PAH) has serious consequence and plasma protein profiles in CHD-PAH are unknown. We aimed to reveal the differential plasma proteins in 272 CHD patients with or without PAH. Various types of CHD-PAH were studied. Differential plasma proteins were first detected by iTRAQ proteomic technology and those with significant clinical relevance were selected for further ELISA validation in new cohort of patients. Among the 190 differential plasma proteins detected by iTRAQ, carbamoyl-phosphate synthetase I (CPSI, related to urea cycle and endogenous nitric oxide production) and complement factor H-related protein 2 (CFHR2, related to complement system and coagulant mechanism) were selected for further ELISA validation in new cohort of 152 patients...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27854133/focusing-on-developmental-origins-and-disease-inception-for-the-prevention-of-pulmonary-hypertension
#2
Bradley A Maron, Steven H Abman
Despite the growing armamentarium of therapies, pulmonary vascular disease continues to cause significant morbidity and mortality in neonates, children and adults in diverse clinical settings. To address this challenge, a strategic shift is underway that emphasizes disease recognition prior to the onset of clinical signs and symptoms when substantial pulmonary vascular remodeling is already present. Specifically, there is growing interest regarding the i) molecular events that define pulmonary vascular disease inception, ii) range of early cardiopulmonary hemodynamic measures that are associated with adverse outcome, and iii) widening utility of exercise for risk-stratification...
November 17, 2016: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/27842563/novel-extracellular-and-nuclear-caspase-1-and-inflammasomes-propagate-inflammation-and-regulate-gene-expression-a-comprehensive-database-mining-study
#3
Luqiao Wang, Hangfei Fu, Gayani Nanayakkara, Yafeng Li, Ying Shao, Candice Johnson, Jiali Cheng, William Y Yang, Fan Yang, Muriel Lavallee, Yanjie Xu, Xiaoshu Cheng, Hang Xi, Jonathan Yi, Jun Yu, Eric T Choi, Hong Wang, Xiaofeng Yang
BACKGROUND: Caspase-1 is present in the cytosol as an inactive zymogen and requires the protein complexes named "inflammasomes" for proteolytic activation. However, it remains unclear whether the proteolytic activity of caspase-1 is confined only to the cytosol where inflammasomes are assembled to convert inactive pro-caspase-1 to active caspase-1. METHODS: We conducted meticulous data analysis methods on proteomic, protein interaction, protein intracellular localization, and gene expressions of 114 experimentally identified caspase-1 substrates and 38 caspase-1 interaction proteins in normal physiological conditions and in various pathologies...
November 14, 2016: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/27796300/parp9-and-parp14-cross-regulate-macrophage-activation-via-stat1-adp-ribosylation
#4
Hiroshi Iwata, Claudia Goettsch, Amitabh Sharma, Piero Ricchiuto, Wilson Wen Bin Goh, Arda Halu, Iwao Yamada, Hideo Yoshida, Takuya Hara, Mei Wei, Noriyuki Inoue, Daiju Fukuda, Alexander Mojcher, Peter C Mattson, Albert-László Barabási, Mark Boothby, Elena Aikawa, Sasha A Singh, Masanori Aikawa
Despite the global impact of macrophage activation in vascular disease, the underlying mechanisms remain obscure. Here we show, with global proteomic analysis of macrophage cell lines treated with either IFNγ or IL-4, that PARP9 and PARP14 regulate macrophage activation. In primary macrophages, PARP9 and PARP14 have opposing roles in macrophage activation. PARP14 silencing induces pro-inflammatory genes and STAT1 phosphorylation in M(IFNγ) cells, whereas it suppresses anti-inflammatory gene expression and STAT6 phosphorylation in M(IL-4) cells...
October 31, 2016: Nature Communications
https://www.readbyqxmd.com/read/27780851/microrna-based-therapy-of-gata2-deficient-vascular-disease
#5
Dorothee Hartmann, Jan Fiedler, Kristina Sonnenschein, Annette Just, Angelika Pfanne, Karina Zimmer, Janet Remke, Ariana Foinquinos, Malte Butzlaff, Katharina Schimmel, Lars Maegdefessel, Denise Hilfiker-Kleiner, Nico Lachmann, Andreas Schober, Natali Froese, Joerg Heineke, Johann Bauersachs, Sandor Batkai, Thomas Thum
BACKGROUND: -The transcription factor GATA2 orchestrates the expression of many endothelial-specific genes illustrating its crucial importance for endothelial cell function. The capacity of this transcription factor in orchestrating endothelial important microRNAs (miRNAs/miR) is unknown. METHODS: -Endothelial GATA2 was functionally analyzed in human endothelial cells in vitro. Endogenous siRNA-mediated knockdown and lentiviral-based overexpression were applied to decipher capacity of GATA2 in regulating cell viability and capillary formation...
October 25, 2016: Circulation
https://www.readbyqxmd.com/read/27718750/platelet-proteomics-applied-to-the-search-for-novel-antiplatelet-therapeutic-targets
#6
Irene Izquierdo, Ángel García
Unwanted platelet activation is associated with numerous diseases, mainly thrombosis-related. In this context, proteomics has emerged as a novel tool with potential for drug target discovery and to scrutinize the effects of antiplatelet drugs. Areas covered: The present review presents the main findings of platelet proteomic studies to date in the context of drug target discovery and perspectives for the future ahead. It includes data and evidences obtained from literature searches on PubMed as well as commentaries derived from the authors' experience and opinions...
October 8, 2016: Expert Review of Proteomics
https://www.readbyqxmd.com/read/27716141/multi-omics-analysis-reveals-regulators-of-the-response-to-pdgf-bb-treatment-in-pulmonary-artery-smooth-muscle-cells
#7
Jidong Chen, Xiaolei Cui, Zhengjiang Qian, Yanjiao Li, Kang Kang, Junle Qu, Li Li, Deming Gou
BACKGROUND: Pulmonary arterial hypertension (PAH) is a lethal disease with pronounced narrowing of pulmonary vessels due to abnormal cell proliferation. The platelet-derived growth factor BB (PDGF-BB) is well known as a potent mitogen for smooth muscle cell proliferation. To better understand how this growth factor regulates pulmonary arterial smooth muscle cells (PASMCs) proliferation, we sought to characterize the response to PDGF-BB stimulation at system-wide levels, including the transcriptome and proteome...
October 6, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27711226/placental-growth-factor-is-secreted-by-the-human-endometrium-and-has-potential-important-functions-during-embryo-development-and-implantation
#8
Natalie K Binder, Jemma Evans, Lois A Salamonsen, David K Gardner, Tu'uhevaha J Kaitu'u-Lino, Natalie J Hannan
Embryo implantation requires synchronized dialogue between the receptive endometrium and activated blastocyst via locally produced soluble mediators. During the mid-secretory (MS) phase of the menstrual cycle, increased glandular secretion into the uterine lumen provides important mediators that modulate the endometrium and support the conceptus during implantation. Previously we demonstrated the importance of vascular endothelial growth factor (VEGF) in the human uterus, particularly with respect to embryo implantation...
2016: PloS One
https://www.readbyqxmd.com/read/27709349/bathing-in-carbon-dioxide-enriched-water-alters-protein-expression-in-keratinocytes-of-skin-tissue-in-rats
#9
Julia Kälsch, Leona L Pott, Atsushi Takeda, Hideo Kumamoto, Dorothe Möllmann, Ali Canbay, Barbara Sitek, Hideo A Baba
Beneficial effects of balneotherapy using naturally occurring carbonated water (CO2 enriched) have been known since the Middle Ages. Although this therapy is clinically applied for peripheral artery disease and skin disorder, the underlying mechanisms are not fully elucidated.Under controlled conditions, rats were bathed in either CO2-enriched water (CO2 content 1200 mg/L) or tap water, both at 37 °C, for 10 min daily over 4 weeks. Proliferation activity was assessed by Ki67 immunohistochemistry of the epidermis of the abdomen...
October 5, 2016: International Journal of Biometeorology
https://www.readbyqxmd.com/read/27662211/cellular-functions-and-gene-and-protein-expression-profiles-in-endothelial-cells-derived-from-moyamoya-disease-specific-ips-cells
#10
Shuji Hamauchi, Hideo Shichinohe, Haruto Uchino, Shigeru Yamaguchi, Naoki Nakayama, Ken Kazumata, Toshiya Osanai, Takeo Abumiya, Kiyohiro Houkin, Takumi Era
BACKGROUND AND PURPOSE: Moyamoya disease (MMD) is a slow, progressive steno-occlusive disease, arising in the terminal portions of the cerebral internal carotid artery. However, the functions and characteristics of the endothelial cells (ECs) in MMD are unknown. We analyzed these features using induced pluripotent stem cell (iPSC)-derived ECs. METHODS: iPSC lines were established from the peripheral blood of three patients with MMD carrying the variant RNF213 R4810K, and three healthy persons used as controls...
2016: PloS One
https://www.readbyqxmd.com/read/27609711/microvesicles-from-brain-extract-treated-mesenchymal-stem-cells-improve-neurological-functions-in-a-rat-model-of-ischemic-stroke
#11
Ji Yong Lee, Eiru Kim, Seong-Mi Choi, Dong-Wook Kim, Kwang Pyo Kim, Insuk Lee, Han-Soo Kim
Transplantation of mesenchymal stem cells (MSCs) was reported to improve functional outcomes in a rat model of ischemic stroke, and subsequent studies suggest that MSC-derived microvesicles (MVs) can replace the beneficial effects of MSCs. Here, we evaluated three different MSC-derived MVs, including MVs from untreated MSCs (MSC-MVs), MVs from MSCs treated with normal rat brain extract (NBE-MSC-MVs), and MVs from MSCs treated with stroke-injured rat brain extract (SBE-MSC-MVs), and tested their effects on ischemic brain injury induced by permanent middle cerebral artery occlusion (pMCAO) in rats...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27601597/comprehensive-proteome-profiling-of-platelet-identified-a-protein-profile-predictive-of-responses-to-an-antiplatelet-agent-sarpogrelate
#12
Hangyeore Lee, Sehyun Chae, Jisook Park, Jingi Bae, Eun-Bi Go, Su-Jin Kim, Hokeun Kim, Daehee Hwang, Sang-Won Lee, Soo-Youn Lee
Sarpogrelate is an antiplatelet agent widely used to treat arterial occlusive diseases. Evaluation of platelet aggregation is essential to monitor therapeutic effects of sarpogrelate. Currently, no molecular signatures are available to evaluate platelet aggregation. Here, we performed comprehensive proteome profiling of platelets collected from 18 subjects before and after sarpogrelate administration using LC-MS/MS analysis coupled with extensive fractionation. Of 5423 proteins detected, we identified 499 proteins affected by sarpogrelate and found that they strongly represented cellular processes related to platelet activation and aggregation, including cell activation, coagulation, and vesicle-mediated transports...
November 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27543695/systems-proteomic-analysis-reveals-that-clusterin-and-tissue-inhibitor-of-metalloproteinases-3-increase-in-leptomeningeal-arteries-affected-by-cerebral-amyloid-angiopathy
#13
A Manousopoulou, M Gatherer, C Smith, J A R Nicoll, C H Woelk, M Johnson, R Kalaria, J Attems, S D Garbis, R O Carare
AIMS: Amyloid beta (Aβ) accumulation in the walls of leptomeningeal arteries as cerebral amyloid angiopathy (CAA) is a major feature of Alzheimer's disease. In this study, we used global quantitative proteomic analysis to examine the hypothesis that the leptomeningeal arteries derived from patients with CAA have a distinct endophenotypic profile compared to those from young and elderly controls. METHODS: Freshly dissected leptomeningeal arteries from the Newcastle Brain Tissue Resource and Edinburgh Sudden Death Brain Bank from seven elderly (82...
October 5, 2016: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/27458111/proteomic-analysis-of-vascular-smooth-muscle-cells-in-physiological-condition-and-in-pulmonary-arterial-hypertension-toward-contractile-versus-synthetic-phenotypes
#14
Alexis Régent, Kim Heang Ly, Sébastien Lofek, Guilhem Clary, Mathieu Tamby, Nicolas Tamas, Christian Federici, Cédric Broussard, Philippe Chafey, Emmanuelle Liaudet-Coopman, Marc Humbert, Frédéric Perros, Luc Mouthon
Vascular smooth muscle cells (VSMCs) are highly specialized cells that regulate vascular tone and participate in vessel remodeling in physiological and pathological conditions. It is unclear why certain vascular pathologies involve one type of vessel and spare others. Our objective was to compare the proteomes of normal human VSMC from aorta (human aortic smooth muscle cells, HAoSMC), umbilical artery (human umbilical artery smooth muscle cells, HUASMC), pulmonary artery (HPASMC), or pulmonary artery VSMC from patients with pulmonary arterial hypertension (PAH-SMC)...
October 2016: Proteomics
https://www.readbyqxmd.com/read/27444336/functional-and-proteomic-investigations-reveal-major-royal-jelly-protein-1-associated-with-anti-hypertension-activity-in-mouse-vascular-smooth-muscle-cells
#15
Pei Fan, Bin Han, Mao Feng, Yu Fang, Lan Zhang, Han Hu, Yue Hao, Yuping Qi, Xiaozhen Zhang, Jianke Li
Vascular smooth muscle cells (VSMCs) are a major cell type of the arterial wall and their functionality is associated with blood pressure regulation. Although royal jelly (RJ) has reported effects on anti-hypertension, the mechanism of blood pressure regulation by major royal jelly protein 1 (MRJP1), the most abundant RJ protein, is still unknown. The mrjp1 gene was inserted into mouse VSMCs to investigate how MRJP1 influences VSMC functionality by functional and proteomic analysis. The expression of MRJP1 in VSMCs significantly reduced cell contraction, migration, and proliferation, suggesting a potential role in decreasing hypertension via action on VSMCs...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27429122/identification-of-novel-biomarker-candidates-for-hypertrophic-cardiomyopathy-and-other-cardiovascular-diseases-leading-to-heart-failure
#16
H Rehulkova, P Rehulka, A Myslivcova Fucikova, J Stulik, R Pudil
In-depth proteome discovery analysis represents new strategy in an effort to identify novel reliable specific protein markers for hypertrophic cardiomyopathy and other life threatening cardiovascular diseases. To systematically identify novel protein biomarkers of cardiovascular diseases with high mortality we employed an isobaric tag for relative and absolute quantitation (iTRAQ) proteome technology to make comparative analysis of plasma samples obtained from patients suffering from non-obstructive hypertrophic cardiomyopathy, stable dilated cardiomyopathy, aortic valve stenosis, chronic stable coronary artery disease and stable arterial hypertension...
July 15, 2016: Physiological Research
https://www.readbyqxmd.com/read/27387989/70-kda-heat-shock-protein-downregulates-dynamin-in-experimental-stroke-a-new-therapeutic-target
#17
Jong Youl Kim, Nuri Kim, Zhen Zheng, Jong Eun Lee, Midori A Yenari
BACKGROUND AND PURPOSE: The 70-kDa heat shock protein (Hsp70) protects brain cells in models of cerebral ischemia. Proteomic screening of mice subjected to middle cerebral artery occlusion identified dynamin as a major downregulated protein in Hsp70-overexpressing mice (Hsp70 transgenic mice). Dynamin-1 is expressed in neurons and participates in neurotransmission, but also transports the death receptor Fas to the cell surface, where it can be bound by its ligand and lead to apoptosis...
August 2016: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/27350024/plasma-proteomic-analysis-of-stable-coronary-artery-disease-indicates-impairment-of-reverse-cholesterol-pathway
#18
Trayambak Basak, Vinay Singh Tanwar, Gourav Bhardwaj, Nitin Bhardwaj, Shadab Ahmad, Gaurav Garg, Sreenivas V, Ganesan Karthikeyan, Sandeep Seth, Shantanu Sengupta
Coronary artery disease (CAD) is one of the largest causes of death worldwide yet the traditional risk factors, although useful in identifying people at high risk, lack the desired predictive accuracy. Techniques like quantitative plasma proteomics holds immense potential to identify newer markers and this study (conducted in three phases) was aimed to identify differentially expressed proteins in stable CAD patients. In the first (discovery) phase, plasma from CAD cases (angiographically proven) and controls were subjected to iTRAQ based proteomic analysis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27347220/aortic-wall-extracellular-matrix-proteins-correlate-with-syntax-score-in-patients-undergoing-coronary-artery-bypass-surgery
#19
Terri Chiong, Esther S H Cheow, Chin C Woo, Xiao Y Lin, Lay W Khin, Chuen N Lee, Mikael Hartman, Siu K Sze, Vitaly A Sorokin
AIMS: The SYNTAX score correlate with major cardiovascular events post-revascularization, although the histopathological basis is unclear. We aim to evaluate the association between syntax score and extracellular matrix histological characteristics of aortic punch tissue obtained during coronary artery bypass surgery (CABG). This analysis compares coronary artery bypass surgery patients with High and Low syntax score which were followed up for one year period. METHODS AND RESULTS: Patients with High (score ≥ 33, (n=77)) and Low Syntax Scores (score ≤ 22, (n=71)) undergoing elective CABG were recruited prospectively...
2016: Open Cardiovascular Medicine Journal
https://www.readbyqxmd.com/read/27341633/omics-based-approaches-to-understand-mechanosensitive-endothelial-biology-and-atherosclerosis
#20
REVIEW
Rachel D Simmons, Sandeep Kumar, Salim Raid Thabet, Sanjoli Sur, Hanjoong Jo
Atherosclerosis is a multifactorial disease that preferentially occurs in arterial regions exposed to d-flow can be used to indicate disturbed flow or disturbed blood flow. The mechanisms by which d-flow induces atherosclerosis involve changes in the transcriptome, methylome, proteome, and metabolome of multiple vascular cells, especially endothelial cells. Initially, we begin with the pathogenesis of atherosclerosis and the changes that occur at multiple levels owing to d-flow, especially in the endothelium...
September 2016: Wiley Interdisciplinary Reviews. Systems Biology and Medicine
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