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https://www.readbyqxmd.com/read/28914569/mutations-in-myeloproliferative-neoplasms-their-significance-and-clinical-use
#1
Fiorella Schischlik, Robert Kralovics
Clonal hematologic diseases of the blood such as polycythemia vera, essential thrombocythemia and primary myelofibrosis belong to the BCR-ABL negative Myeloproliferative Neoplasms (MPN). These diseases are characterized by clonal expansion of hematopoietic precursor cells followed by increased production of differentiated cells of the myeloid lineage. Initiation of clonal hematopoiesis, formation of a clinical phenotype as well as disease progression form part of MPN disease evolution. The disease is driven by acquired somatic mutations in critical pathways such as cytokine signaling, epigenetic regulation, RNA splicing, and transcription factor signaling...
September 15, 2017: Expert Review of Hematology
https://www.readbyqxmd.com/read/28913558/only-setbp1-hotspot-mutations-are-associated-with-refractory-disease-in-myeloid-malignancies
#2
Nils Winkelmann, Vivien Schäfer, Jenny Rinke, Alexander Kaiser, Philipp Ernst, Sebastian Scholl, Andreas Hochhaus, Thomas Ernst
INTRODUCTION: SETBP1 mutations have been established as a diagnostic marker in myeloid malignancies and are associated with inferior survival. Since there is limited data on their clinical impact and stability during disease progression, we sought to investigate the relationship between SETBP1 mutations and disease evolution. METHODS: Bidirectional Sanger sequencing of the SETBP1 gene was performed for 442 unselected patients with World Health Organization (WHO) defined myeloid disorders...
September 14, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28904945/effectiveness-of-the-thermal-treatments-used-for-curd-stretching-in-the-inactivation-of-shiga-toxin-producing-o157-and-o26-escherichia-coli
#3
M Trevisani, A Valero, R Mancusi
The kneading treatment of the fresh curd in hot water is a critical control point in the manufacturing of mozzarella. Factors such as the ratio between hot water and curd mass, the rheological properties, and the mixing and kneading activity affect the processing time and the internal temperature of the curd. The aim of this study was to investigate the effect of thermal treatments on the fate of Shiga toxin-producing Escherichia coli (STEC). Nine curd samples (weight 160-270 g) were artificially contaminated with O157 or O26 STEC and stretched in hot water (90-95°C) for 5-10 min...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28903325/cxcl12-cxcr4-pathway-is-activated-by-oncogenic-jak2-in-a-pi3k-dependent-manner
#4
Hadjer Abdelouahab, Yanyan Zhang, Monika Wittner, Shinya Oishi, Nobutaka Fujii, Rodolphe Besancenot, Isabelle Plo, Vincent Ribrag, Eric Solary, William Vainchenker, Giovanni Barosi, Fawzia Louache
JAK2 activation is the driver mechanism in BCR-ABL-negative myeloproliferative neoplasms (MPN). These diseases are characterized by an abnormal retention of hematopoietic stem cells within the bone marrow microenvironment and their increased trafficking to extramedullary sites. The CXCL12/CXCR4 axis plays a central role in hematopoietic stem cell/ progenitor trafficking and retention in hematopoietic sites. The present study explores the crosstalk between JAK2 and CXCL12/CXCR4 signaling pathways in MPN. We show that JAK2, activated by either MPL-W515L expression or cytokine stimulation, cooperates with CXCL12/CXCR4 signaling to increase the chemotactic response of human cell lines and primary CD34(+) cells through an increased phosphatidylinositol-3-kinase (PI3K) signaling...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28888938/occurrence-of-vibrio-cholerae-in-water-reservoirs-of-burkina-faso
#5
Kaboré Saidou, Philippe Cecchi, Thomas Mosser, Toubiana Mylène, Traoré Oumar, Aboubakar S Ouattara, Alfred S Traoré, Nicolas Barro, Rita R Colwell, Patrick Monfort
Africa is currently an important region in which cholera epidemics occur. Little is known about the presence of Vibriocholerae in freshwater bodies in Africa. There are ca. 1,700 lakes and reservoirs in Burkina Faso, most of which have been built within recent decades to secure water resources. The purpose of this study was to investigate the presence of V. cholera in the water of reservoirs, using the most-probable-number polymerase chain reaction. Results showed that V. cholera could be detected in water samples collected from 14 of 39 sampled reservoirs...
September 6, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28876265/biomass-granulation-in-an-upflow-anaerobic-sludge-blanket-reactor-treating-500-m-3-day-low-strength-sewage-and-post-treatment-in-high-rate-algal-pond
#6
Pritha Chatterjee, M M Ghangrekar
A pilot-scale upflow anaerobic sludge blanket-moving bed biofilm (UASB-MBB) reactor followed by a high-rate algal pond (HRAP) was designed and operated to remove organic matter, nutrients and pathogens from sewage and to facilitate reuse. For an influent chemical oxygen demand (COD) concentration of 233 ± 20 mg/L, final effluent COD was 50 ± 6 mg/L. Successful biomass granulation was observed in the sludge bed of the upflow anaerobic sludge blanket (UASB) reactor after 5 months of operation. Ammonia removal in HRAP was 85...
September 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28871463/specific-effects-of-prenatal-dehp-exposure-on-neuroendocrine-gene-expression-in-the-developing-hypothalamus-of-male-rats
#7
Na Gao, Ruixia Hu, Yujing Huang, Long Dao, Caifeng Zhang, Yongzhe Liu, Lina Wu, Xutong Wang, Weiling Yin, Andrea C Gore, Zengrong Sun
Endocrine disrupting chemicals may disrupt developing neuroendocrine systems, especially when the exposure occurs during a critical period. This study aimed to investigate whether prenatal exposure to di-(2-ethylhexyl) phthalate (DEHP), a major component of plasticizers used worldwide, disrupted the development of a network of genes important for neuroendocrine function in male rats. Pregnant rats were treated with corn oil (vehicle control), 2, 10 or 50 mg/kg DEHP by gavage from gestational day 14 to 19. The neuroendocrine gene expressions were quantified using a 48-gene Taqman qPCR array in the whole hypothalamus of neonatal rats (postnatal day 1) and in the anteroventral periventricular nucleus (AVPV), medial preoptic nucleus (MPN) and arcuate nucleus (ARC) of adult rats (postnatal day 70)...
September 4, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28867170/assessment-of-pit-latrines-in-a-peri-urban-community-in-kwazulu-natal-south-africa-as-a-source-of-antibiotic-resistant-e-coli-strains
#8
Lorika S Beukes, Tracy L B King, Stefan Schmidt
Due to the frequent use of antibiotics and recurring illnesses related to multidrug-resistant (MDR) bacteria in South Africa, we determined if MDR Escherichia coli were present in pit latrine fecal sludge samples obtained from a peri-urban community in KwaZulu-Natal, South Africa. The abundance of E. coli in pit latrine samples was established using a most probable number (MPN) method with species confirmation done using biochemical tests and polymerase chain reaction (PCR). Forty-four randomly selected E. coli pit latrine isolates were further characterized, using the European committee on antimicrobial susceptibility testing (EUCAST) disk diffusion method to establish antibiotic resistance profiles for these E...
August 12, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28859112/mathematical-modelling-as-a-proof-of-concept-for-mpns-as-a-human-inflammation-model-for-cancer-development
#9
Morten Andersen, Zamra Sajid, Rasmus K Pedersen, Johanne Gudmand-Hoeyer, Christina Ellervik, Vibe Skov, Lasse Kjær, Niels Pallisgaard, Torben A Kruse, Mads Thomassen, Jesper Troelsen, Hans Carl Hasselbalch, Johnny T Ottesen
The chronic Philadelphia-negative myeloproliferative neoplasms (MPNs) are acquired stem cell neoplasms which ultimately may transform to acute myelogenous leukemia. Most recently, chronic inflammation has been described as an important factor for the development and progression of MPNs in the biological continuum from early cancer stage to the advanced myelofibrosis stage, the MPNs being described as "A Human Inflammation Model for Cancer Development". This novel concept has been built upon clinical, experimental, genomic, immunological and not least epidemiological studies...
2017: PloS One
https://www.readbyqxmd.com/read/28855471/estimation-of-microbial-concentration-in-food-products-from-qualitative-microbiological-test-data-with-the-mpn-technique
#10
Hiroshi Fujikawa
Microbial concentration in samples of a food product lot has been generally assumed to follow the log-normal distribution in food sampling, but this distribution cannot accommodate the concentration of zero. In the present study, first, a probabilistic study with the most probable number (MPN) technique was done for a target microbe present at a low (or zero) concentration in food products. Namely, based on the number of target pathogen-positive samples in the total samples of a product found by a qualitative, microbiological examination, the concentration of the pathogen in the product was estimated by means of the MPN technique...
2017: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/28843161/ruxolitinib-for-the-management-of-myelofibrosis-results-of-an-international-physician-survey
#11
Martin H Ellis, Maya Koren-Michowitz, Noa Lavi, Alessandro M Vannucchi, Ruben Mesa, Claire N Harrison
BACKGROUND: Ruxolitinib is established as treatment for symptomatic myeloproliferative neoplasm (MPN)-associated myelofibrosis. The strict inclusion and exclusion criteria and dose modification rules that applied to the COMFORTI and II studies that led to the licensing of ruxolitinib are not always applicable to routine clinical practice. Thus physicians now face decisions regarding ruxolitinib use that were not addressed in these pivotal trials. METHODS: We performed an online survey of hematologists practicing in Europe, Israel, the United Kingdom and the United States...
August 10, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28840290/functionalization-of-mgzno-nanorod-films-and-characterization-by-ftir-microscopic-imaging
#12
Yuan Chen, Qihong Zhang, Carol Flach, Richard Mendelsohn, Elena Galoppini, Pavel Ivanoff Reyes, Keyang Yang, Rui Li, Guangyuan Li, Yicheng Lu
Metal organic chemical vapor deposition grown films consisting of MgxZn1-xO (4% < x < 5%) nanorod arrays (MgZnOnano) were functionalized with 11-azidoundecanoic acid (1). The MgZnOnano was used instead of pure ZnO to take advantage of the etching resistance of the MgZnOnano during the binding and subsequent sensing device fabrication processes of sensor devices, while the low Mg composition level ensures that selected ZnO properties useful for sensors development, such as piezoelectricity, are retained...
August 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28831961/efficient-milking-hygiene-reduces-bacterial-spore-contamination-in-milk
#13
Luciana Bava, Stefania Colombini, Maddalena Zucali, Marilù Decimo, Stefano Morandi, Tiziana Silvetti, Milena Brasca, Alberto Tamburini, G Matteo Crovetto, Anna Sandrucci
Clostridia in the milk can lead to late blowing, a cheese defect. Clostridia are ubiquitous, deriving from both the farm environment and the feed ingested by the cows, and are transferred into the milk through faecal contamination. Our aim was to investigate the effect of different in-parlour practices on the content of anaerobic spore-forming bacteria in milk, and to monitor the variation in spore content in the feed and environment. The experiment, conducted in an experimental dairy during autumn, was repeated in exactly the same way for two consecutive years...
August 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28828906/mpn10-score-and-survival-of-molecularly-annotated-myeloproliferative-neoplasm-patients
#14
Yasser H ElNahass, Hossam K Mahmoud, Mervat M Mattar, Omar A Fahmy, Mohamed A Samra, Raafat M Abdelfattah, Fatma A ElRefaey, Hala M Fahmy, Gamal M Fathy, Ahmed Abdulgawad, Mohamed AbdelKader, Hend N Elleithy, Mona Gamil, Mohamed Talaat, Heba A Nader, Wafaa H ElMetnawy
JAK2, CALR, MPL and triple-negative mutational status has a direct impact on symptom severity and disease burden assessed by MPN10 score in myeloproliferative neoplasms (MPNs). Among 93 patients; median MPN10 score was 48 (5-76) in JAK2 mutants versus 25 (4-80) in JAK2 negative (p < .001); 22.5 (4-65) in CALR mutants versus 35 (5-80) in CALR negative (p < .050) and 21 (10-48) in triple negative versus 40 (4-80) in JAK2/CALR/MPL mutants (p < .001). At three years, progression free and overall survival of JAK2-positive versus JAK2-negative patients were 62% versus 100% (p < ...
August 22, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/28822933/occurrence-of-vibrio-species-beta-lactam-resistant-vibrio-species-and-indicator-bacteria-in-ballast-and-port-waters-of-a-tropical-harbor
#15
Charmaine Ng, Shin Giek Goh, Nazanin Saeidi, William A Gerhard, Claudia K Gunsch, Karina Yew Hoong Gin
Ballast water discharges are potential sources for the spread of invasive and pathogenic aquatic organisms. Ballast waters from six ships docked in the Port of Singapore were tested to determine if indictor organisms fell within proposed standards for ballast water discharge according to regulation D-2 of the Ballast Water Management Convention (BWMC) guidelines. Vibrio species were cultured on media supplemented with beta-lactam antibiotics to determine the presence of antibiotic resistant Vibrio species in the ballast waters of these vessels...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822056/patient-reported-outcomes-in-myelodysplastic-syndromes-and-mds-mpn-overlap-syndromes-stepping-onto-the-stage-with-changing-times
#16
REVIEW
Sagar S Patel, Aaron T Gerds
Quality of life (QOL) and symptom burden are important measures captured by patient-reported outcomes (PROs). Myelodysplastic and myelodysplastic/myeloproliferative (MDS/MPN) neoplasm overlap syndromes are notable for significant morbidity and mortality, including a wide spectrum of physical and psychosocial effects. Thus, the development and application of PROs can provide meaningful information to facilitate communication and assist in follow up care. Disease-specific measures can more accurately reflect the full breadth of functional restrictions and symptoms...
August 18, 2017: Current Hematologic Malignancy Reports
https://www.readbyqxmd.com/read/28819248/a-portable-microfluidic-platform-for-rapid-molecular-diagnostic-testing-of-patients-with-myeloproliferative-neoplasms
#17
Hua Wang, Xinju Zhang, Xiao Xu, Qunfeng Zhang, Hengliang Wang, Dong Li, Zhihua Kang, Zhiyuan Wu, Yigui Tang, Zhenhua An, Ming Guan
The ability to simultaneously detect JAK2 V617F and MPL W515K/L mutations would substantially improve the early diagnosis of myeloproliferative neoplasms (MPNs) and decrease the risk of arterial thrombosis. The goal of this study is to achieve a point of care testing platform for simultaneous analysis of major genetic alterations in MPN. Here, we report a microfluidic platform including a glass capillary containing polypropylene matrix that extracts genomic DNA from a drop of whole blood, a microchip for simultaneous multi-gene mutation screening, and a handheld battery-powered heating device...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28817122/single-cell-mechanogenetics-using-monovalent-magnetoplasmonic-nanoparticles
#18
Ji-Wook Kim, Daeha Seo, Jung-Uk Lee, Kaden M Southard, Yongjun Lim, Daehyun Kim, Zev J Gartner, Young-Wook Jun, Jinwoo Cheon
Spatiotemporal interrogation of signal transduction at the single-cell level is necessary to answer a host of important biological questions. This protocol describes a nanotechnology-based single-cell and single-molecule perturbation tool, termed mechanogenetics, that enables precise spatial and mechanical control over genetically encoded cell-surface receptors in live cells. The key components of this tool are a magnetoplasmonic nanoparticle (MPN) actuator that delivers defined spatial and mechanical cues to receptors through target-specific one-to-one engagement and a micromagnetic tweezers (μMT) that remotely controls the magnitude of force exerted on a single MPN...
September 2017: Nature Protocols
https://www.readbyqxmd.com/read/28797634/ecological-distribution-of-extremely-thermophilic-bacteria-belonging-to-the-genus-calditerricola-using-the-novel-enrichment-mpn-pcr-method
#19
Kathrina Mae Bienes, Minoru Ito, Kota Shiotsuka, Sachi Yamaguchi, Taiki Fujioka, Yukihiro Tashiro, Kenji Sakai
A unique compost called Satsuma soil is produced from sewage sludge by a hyperthermal composting process in Kagoshima City, Japan. The composting process is carried out at a controlled temperature of at least 80°C and the resulting compost might be useful for recycling sustainable agricultural products. The extremely thermophilic bacterial genus Calditerricola was initially isolated from the high-temperature compost. Likewise, the bacteria were previously isolated from material sludge. It is believed that bacteria in this genus might be involved in the hyperthermal composting process...
August 7, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28796790/mesenchymal-stromal-cells-msc-from-jak2-myeloproliferative-neoplasms-differ-from-normal-msc-and-contribute-to-the-maintenance-of-neoplastic-hematopoiesis
#20
Teresa L Ramos, Luis Ignacio Sánchez-Abarca, Beatriz Rosón-Burgo, Alba Redondo, Ana Rico, Silvia Preciado, Rebeca Ortega, Concepción Rodríguez, Sandra Muntión, Ángel Hernández-Hernández, Javier De Las Rivas, Marcos González, José Ramón González Porras, Consuelo Del Cañizo, Fermín Sánchez-Guijo
There is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expression profile of BM-MSC from healthy donors (HD-MSC) and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation and differentiation capacity between both groups. However, BM-MSC from MPN expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas CD105 was lower when compared to controls...
2017: PloS One
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