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Collagen crosslinking

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https://www.readbyqxmd.com/read/29147094/physicochemical-properties-of-gelatin-extracted-from-buffalo-hide-pretreated-with-different-acids
#1
Sri Mulyani, Francis M C Sigit Setyabudi, Yudi Pranoto, Umar Santoso
The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components. Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29146187/lox-related-collagen-crosslink-changes-act-as-an-initiator-of-bone-fragility-in-a-zdf-rats-model
#2
Xun Xiao, Jie Ren, Jun Chen, Zhaohui Liu, Ye Tian, Neel R Nabar, Min Wang, Liang Hao
Diabetes mellitus type 2 (DM2) results in bone abnormalities that manifest as increased bone fragility. Bone consists of two phases, the mineral phase and the matrix phase, and disorders in both are seen in DM2. However, the phase in which DM2 mediated bone fragility is initiated is still unknown. In this study, a male Zucker diabetic fatty (fa/fa) (ZDF) rat model was used to investigate the underlying mechanism initiating DM2 mediated bone fragility. The fracture surface morphology, pre- and post-yield bone mechanical behavior, insoluble collagen volume, lysyl oxidase family (LOX) enzyme levels and correlation analysis was performed to determine the relationship between insoluble collagen and post-yield behavior...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29135319/crosslinking-enzyme-lysyl-oxidase-modulates-scleral-remodeling-in-form-deprivation-myopia
#3
Ying Yuan, Min Li, Qingzhong Chen, Rao Me, Yunjie Yu, Qing Gu, Guangsen Shi, Bilian Ke
PURPOSE: Scleral remodeling causes the excessive ocular elongation that underlies myopia. Lysyl oxidase (LOX), a copper-containing amine oxidase, can catalyze collagen and elastin crosslinking. The purpose of this study was to investigate the role of LOX in scleral remodeling in form-deprivation myopia (FDM). METHODS: Seventy-five guinea pigs were randomly divided into five groups as follows: a normal control group, an FDM group, an FDM plus β-aminopropionitrile (BAPN) group, an FDM plus TGF-β1 (TGF-β1) group, and an FDM plus vehicle group...
November 14, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29133833/effect-of-soy-on-bone-turn-over-markers-in-men-with-type-2-diabetes-and-hypogonadism-a-randomised-controlled-study
#4
T Sathyapalan, M Aye, A S Rigby, W D Fraser, E S Kilpatrick, S L Atkin
Type 2 diabetes (T2DM) is associated with increased risk of fractures. Soy supplementation has been shown to have a beneficial effect on bone turnover markers (BTM) in postmenopausal women. However, the effect of soy supplementation on BTM in T2DM and particularly in men is unclear. We performed an analysis of a randomized double blind parallel study of 200 men with T2DM treated with soy, either with or without isoflavones. Outcome measures were type I collagen crosslinked beta C-telopeptide (βCTX), and type 1 procollagen-N-propeptide (P1NP)...
November 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29132046/stress-relaxing-hyaluronic-acid-collagen-hydrogels-promote-cell-spreading-fiber-remodeling-and-focal-adhesion-formation-in-3d-cell-culture
#5
Junzhe Lou, Ryan Stowers, Sungmin Nam, Yan Xia, Ovijit Chaudhuri
The physical and architectural cues of the extracellular matrix (ECM) play a critical role in regulating important cellular functions such as spreading, migration, proliferation, and differentiation. Natural ECM is a complex viscoelastic scaffold composed of various distinct components that are often organized into a fibrillar microstructure. Hydrogels are frequently used as synthetic ECMs for 3D cell culture, but are typically elastic, due to covalent crosslinking, and non-fibrillar. Recent work has revealed the importance of stress relaxation in viscoelastic hydrogels in regulating biological processes such as spreading and differentiation, but these studies all utilize synthetic ECM hydrogels that are non-fibrillar...
November 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/29130610/the-effects-of-icariin-on-wound-healing-of-extraction-sites-with-administration-of-zoledronic-and-dexamethasone-a-rat-model-study
#6
Jie Liu, Nikos Mattheos, Cheng Su, Chuanxi Deng, Nanyu Luo, Zekun Wang, Hua Tang
OBJECTIVE: This study was intended to investigate the effects of Icariin on the healing of tooth extraction sites under systemic administration of Zoledronic and Dexamethasone. METHOD: Thirty female rats underwent bilateral ovariectomy and were randomly assigned to 5 groups: SS group received weekly injection of saline while ZD, ZD+LICA, ZD+MICA and ZD+HICA groups received Zoledronic with Dexamethasone for 8 weeks. One week later, mandibular first molars were extracted in all groups...
November 11, 2017: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/29128532/laponite-nanoparticle-associated-silated-hydroxypropylmethyl-cellulose-as-an-injectable-reinforced-interpenetrating-network-hydrogel-for-cartilage-tissue-engineering
#7
Cécile Boyer, Lara Figueiredo, Richard Pace, Julie Lesoeur, Thierry Rouillon, Catherine Le Visage, Jean-François Tassin, Pierre Weiss, Jérôme Guicheux, Gildas Rethore
Articular cartilage is a connective tissue which does not spontaneously heal. To address this issue, biomaterial-assisted cell therapy has been researched with promising advances. The lack of strong mechanical properties is still a concern despite significant progress in three-dimensional scaffolds. This article's objective was to develop a composite hydrogel using a small amount of nano-reinforcement clay known as laponites. These laponites were capable of self-setting within the gel structure of the silated hydroxylpropylmethyl cellulose (Si-HPMC) hydrogel...
November 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29127344/catabolic-effects-of-human-pth-1-34-on-bone-requirement-of-monocyte-chemoattractant-protein-1-in-murine-model-of-hyperparathyroidism
#8
Jawed A Siddiqui, Joshua Johnson, Carole Le Henaff, Claudine L Bitel, Joseph A Tamasi, Nicola C Partridge
The bone catabolic actions of parathyroid hormone (PTH) are seen in patients with hyperparathyroidism, or with infusion of PTH in rodents. We have previously shown that the chemokine, monocyte chemoattractant protein-1 (MCP-1), is a mediator of PTH's anabolic effects on bone. To determine its role in PTH's catabolic effects, we continuously infused female wild-type (WT) and MCP-1(-/-) mice with hPTH or vehicle. Microcomputed tomography (µCT) analysis of cortical bone showed that hPTH-infusion induced significant bone loss in WT mice...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29122823/comparative-evaluation-of-progression-rate-in-keratoconus-before-and-after-collagen-crosslinking
#9
Yu Meng Wang, Tommy Cy Chan, Marco C Y Yu, Vishal Jhanji
PURPOSE: To compare the rate of disease progression in keratoconus before and after corneal collagen crosslinking (CXL). METHODS: 145 eyes were followed without CXL (no-CXL group) for a median duration of 31 months whereas 45 eyes were followed up for 41 months before (pre-CXL) and after (post-CXL) accelerated, epithelium-off crosslinking. Progression was defined based on significant slope found in linear mixed effect models against time. Swept-source optical coherence tomography was used for measurement of anterior steep keratometry, anterior flat keratometry (Ant Kf), anterior average keratometry (Ant Avg K); posterior steep keratometry, posteriorflat keratometry (Post Kf), posterior average keratometry (Post Avg K) and corneal thickness...
November 9, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29115860/lysyl-oxidase-like-1-protein-deficiency-protects-mice-from-adtgf-%C3%AE-1-induced-pulmonary-fibrosis
#10
Pierre-Simon Bellaye, Chiko Shimbori, Chandak Upagupta, Seidai Sato, Wei Shi, Jack Gauldie, Kjetil Ask, Martin Kolb
Idiopathic pulmonary fibrosis (IPF) is a progressive disease characterised by excessive deposition of extracellular matrix (ECM) in the lung parenchyma. The abnormal ECM deposition slowly overtakes normal lung tissue disturbing gas-exchange and leading to respiratory failure and death. ECM cross-linking and subsequent stiffening is thought to be a major contributor of disease progression and also promotes the activation of TGF-β1, one of the main pro-fibrotic growth factors. Lysyl oxidase-like1 (LOXL1) belongs to the crosslinking enzyme family and has been shown to be upregulated in active fibrotic regions of bleomycin-treated mice and IPF patients...
November 8, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29108850/tissue-viscoelasticity-is-related-to-tissue-composition-but-may-not-fully-predict-the-apparent-level-viscoelasticity-in-human-trabecular-bone-an-experimental-and-finite-element-study
#11
X Ojanen, P Tanska, M K H Malo, H Isaksson, S P Väänänen, A P Koistinen, L Grassi, S P Magnusson, S M Ribel-Madsen, R K Korhonen, J S Jurvelin, J Töyräs
Trabecular bone is viscoelastic under dynamic loading. However, it is unclear how tissue viscoelasticity controls viscoelasticity at the apparent-level. In this study, viscoelasticity of cylindrical human trabecular bone samples (n=11, male, age 18-78 years) from 11 proximal femurs were characterized using dynamic and stress-relaxation testing at the apparent-level and with creep nanoindentation at the tissue-level. In addition, bone tissue elasticity was determined using scanning acoustic microscope (SAM)...
October 13, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29107125/degradation-of-extracellular-matrix-regulates-osteoblast-migration-a-microfluidic-based-study
#12
N Movilla, C Borau, C Valero, J M García-Aznar
Bone regeneration is strongly dependent on the capacity of cells to move in a 3D microenvironment, where a large cascade of signals is activated. To improve the understanding of this complex process and to advance in the knowledge of the role of each specific signal, it is fundamental to analyze the impact of each factor independently. Microfluidic-based cell culture is an appropriate technology to achieve this objective, because it allows recreating realistic 3D local microenvironments by taking into account the extracellular matrix, cells and chemical gradients in an independent or combined scenario...
October 26, 2017: Bone
https://www.readbyqxmd.com/read/29107054/selecting-the-correct-cellular-model-for-assessing-of-the-biological-response-of-collagen-based-biomaterials
#13
Natalia Davidenko, Samir Hamaia, Daniel V Bax, Jean-Daniel Malcor, Carlos F Schuster, Donald Gullberg, Richard W Farndale, Serena M Best, Ruth E Cameron
Accurate evaluation of the biological performance of biomaterials requires the correct assessment of their native-like cell ligation properties. However, cell attachment studies often overlook the details of the substrate-cell binding mechanisms, be they integrin-mediated or non-specific, and ignore the class- and species-specificities of the cell adhesion receptor involved. In this work we have used different collagen (Col) substrates (fibrillar collagens I, II and III and network-forming Col IV), containing different affinity cell-recognition motifs, to establish the influence of the receptor identity and species-specificity on collagen-cell interactive properties...
October 26, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29103492/a-composite-hydrogel-of-chitosan-heparin-poly-%C3%AE-glutamic-acid-loaded-with-superoxide-dismutase-for-wound-healing
#14
Lin Zhang, Yina Ma, Xiaochen Pan, Siyuan Chen, Huahong Zhuang, Shufang Wang
In chronic wounds, severe oxidative stress hampers the healing of wounds. This study constructed a novel antioxidant-loaded hydrogel for healing diabetic wounds. First, composite hydrogels consisting of chitosan, heparin and poly (γ-glutamic acid) with different ratios were prepared through electrostatic interaction. These hydrogels exhibited good three-dimensional network structures, and the porosity of the hydrogels decreased as the crosslinking density increased. The hydrogels possessed good swelling capacity, and showed typical viscoelastic behavior and good mechanical property in rheological test...
January 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29099978/glucose-dependent-insulinotropic-polypeptide-gip-inhibits-bone-resorption-independently-of-insulin-and-glycemia
#15
Mikkel B Christensen, Asger Lund, Salvatore Calanna, Niklas R Jørgensen, Jens J Holst, Tina Vilsbøll, Filip K Knop
Context: The gut hormone GIP causes postprandial insulin release and inhibits bone resorption assessed by carboxy-terminal collagen crosslinks (CTX). Objective: To clarify if GIP affects biomarkers involved in bone homeostasis independently of insulin release and glycemic level. Design: Randomized, double-blinded, cross-over study with 5 study days. Patients: Ten lean male C-peptide-negative patients with type 1 diabetes...
November 1, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29091110/evaluation-in-vivo-of-biocompatibility-of-differents-resin-modified-cements-for-bonding-orthodontic-bands
#16
Janaina A Mesquita, Rogério Lacerda-Santos, Gêisa A M Sampaio, Gustavo P Godoy, Cassiano F W Nonaka, Pollianna M Alves
The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of resin-modified glass ionomer cements. Sixty male Wistar rats were selected and divided into four groups: Control Group; Crosslink Group; RMO Group and Transbond Group. The materials were inserted into rat subcutaneous tissue. After time intervals of 7, 15 and 30 days morphological analyses were performed. The histological parameters assessed were: inflammatory infiltrate intensity; reaction of multinucleated giant cells; edema; necrosis; granulation reaction; young fibroblasts and collagenization...
October 26, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29082102/custom-built-nonlinear-optical-crosslinking-nlo-cxl-device-capable-of-producing-mechanical-stiffening-in-ex-vivo-rabbit-corneas
#17
Samantha M Bradford, Eric R Mikula, Dongyul Chai, Donald J Brown, Tibor Juhasz, James V Jester
The purpose of this study was to develop and test a nonlinear optical device to photoactivate riboflavin to produce spatially controlled collagen crosslinking and mechanical stiffening within the cornea. A nonlinear optical device using a variable numerical aperture objective was built and coupled to a Chameleon femtosecond laser. Ex vivo rabbit eyes were then saturated with riboflavin and scanned with various scanning parameters over a 4 mm area in the central cornea. Effectiveness of NLO CXL was assessed by evaluating corneal collagen auto fluorescence (CAF)...
October 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29081866/conventional-versus-accelerated-collagen-cross-linking-for-keratoconus-a-comparison-of-visual-refractive-topographic-and-biomechanical-outcomes
#18
Jyh Haur Woo, Jayant Venkatramani Iyer, Li Lim, M Htoon Hla, Jodhbir S Mehta, Cordelia Ml Chan, Donald Th Tan
OBJECTIVE: The aim was to compare the visual, refractive, topographic and biomechanical outcomes in patients with progressive keratoconus treated with either conventional or accelerated crosslinking at one year follow up. METHODS: It is a prospective, non-randomised interventional study of 76 patients who underwent conventional (CXL; 3mW/cm(2) for 30 minutes) or accelerated cross linking (KXL; 30mW/cm(2) for 4 minutes) for progressive keratoconus. Baseline and postoperative visual acuity, manifest refraction, corneal topography, pachymetry, endothelial cell density and biomechanical parameters of corneal hysteresis and corneal resistance factor were evaluated and compared...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/29058055/effects-of-collagen-crosslinkers-on-dentine-a-literature-review
#19
REVIEW
J Cai, J E A Palamara, M F Burrow
This aim of this review is to explore the current research related to crosslinking agents used on dentine. A systematic search of publications in PubMed and Web of Science databases was performed. Further retrieval was conducted using the search terms of specific names of crosslinkers. Reviews, conference abstracts, dissertation and theses, non-English articles, studies of intrinsic crosslinking of dentine, studies of adhesives without specific crosslinker components, studies of crosslinker applications in other collagenous tissues or tooth-like structures and irrelevant studies were excluded...
October 20, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/29056373/jte-852-a-novel-spleen-tyrosine-kinase-inhibitor-blocks-immunoglobulin-g-mediated-cellular-responses-and-autoimmune-reactions-in-vivo
#20
Toshinobu Kato, Takeshi Ohta, Hidenori Iwasaki, Hatsue Kobayashi, Akira Matsuo, Takahiro Hata, Mutsuyoshi Matsushita
AIMS: Immune and inflammatory responses mediated by immunoglobulin (Ig) G are largely responsible for the pathogenesis of autoimmune diseases. Spleen tyrosine kinase (Syk) plays a pivotal role in the IgG-mediated responses; therefore, Syk has emerged as a new therapeutic target for the treatment of autoimmune diseases. In this study, we investigated the inhibitory actions of JTE-852, a novel Syk inhibitor, on IgG-mediated cellular responses and autoimmune reactions in vivo. MAIN METHODS: We examined mediator secretion from human monocytes...
October 19, 2017: Life Sciences
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