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Collagen crosslinking

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https://www.readbyqxmd.com/read/28088646/bone-microarchitecture-in-adolescent-boys-with-autism-spectrum-disorder
#1
Ann M Neumeyer, Natalia Cano Sokoloff, Erin McDonnell, Eric A Macklin, Christopher J McDougle, Madhusmita Misra
BACKGROUND: Boys with autism spectrum disorder (ASD) have lower areal bone mineral density (aBMD) than typically developing controls (TDC). Studies of volumetric BMD (vBMD) and bone microarchitecture provide information about fracture risk beyond that provided by aBMD but are currently lacking in ASD. OBJECTIVES: To assess ultradistal radius and distal tibia vBMD, bone microarchitecture and strength estimates in adolescent boys with ASD compared to TDC. DESIGN/METHODS: Cross-sectional study of 34 boys (16 ASD, 18 TDC) that assessed (i) aBMD at the whole body (WB), WB less head (WBLH), hip and spine using dual X-ray absorptiometry (DXA), (ii) vBMD and bone microarchitecture at the ultradistal radius and distal tibia using high-resolution peripheral quantitative CT (HRpQCT), and (iii) bone strength estimates (stiffness and failure load) using micro-finite element analysis (FEA)...
January 11, 2017: Bone
https://www.readbyqxmd.com/read/28077050/a-novel-human-like-collagen-hemostatic-sponge-with-uniform-morphology-good-biodegradability-and-biocompatibility
#2
Xijuan Jiang, Ya Wang, Daidi Fan, Chenhui Zhu, Lin Liu, Zhiguang Duan
Biodegradable sponges, as a promising hemostatic biomaterial, has been clinically required over the past decades. Current hemostatic sponges are generally prepared by crosslinking and freeze-drying, but the quality control or biocompatibility is often unsatisfactory due to the freezing-caused morphological non-uniformity and the toxicity of raw materials or cross-linkers. The crosslinking often greatly retards the degradation of the sponges and thus affects the healing of the wound. In this work, we prepared a novel hemostatic sponge using human-like collagen and glutamine transaminase (non-toxic cross-linker) and optimized its morphology via "two-step" freezing instead of conventional "one-step" freezing...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28076887/in-vivo-bone-tunnel-evaluation-of-nanoparticle-grafts-using-an-acl-reconstruction-rabbit-model
#3
S A Grant, S E Smith, H Schmidt, F Pfeiffer, K Kuroki, S Sherman, R A White, D A Grant
Acellular human gracilis tendons conjugated with gold nanoparticles (AuNP) and hydroxyapatite nanoparticles (nano-HAp) were used as a graft in an anterior cruciate ligament (ACL) reconstruction rabbit model. The ACLs of 11 New Zealand rabbits were reconstructed using grafts conjugated without nanoparticles, with AuNP only, and with both AuNP and nano-HAp. Semi-quantitative histological scoring of bone tunnel portion of grafts was performed after 14 weeks. Bone tunnels were scored for graft degeneration, graft remodeling, percentage of new host fibrous connective, collateral connection, head-to-head connection, graft collagen fiber organization, new host fibrous connective tissue organization, and graft and interface vascularity...
January 11, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28073888/selective-targeting-of-lysyl-oxidase-like-2-loxl2-suppresses-hepatic-fibrosis-progression-and-accelerates-its-reversal
#4
Naoki Ikenaga, Zhen-Wei Peng, Kahini A Vaid, Susan B Liu, Shuhei Yoshida, Deanna Y Sverdlov, Amanda Mikels-Vigdal, Victoria Smith, Detlef Schuppan, Yury V Popov
BACKGROUND/AIMS: We studied the role of lysyl oxidase-like 2 (LOXL2) in collagen crosslinking and hepatic progenitor cell (HPC) differentiation, and the therapeutic efficacy of a LOXL2-blocking monoclonal antibody on liver fibrosis progression/reversal in mice. METHODS: Anti-LOXL2 antibody, control antilysyl oxidase antibody or placebo was administered during thioacetamide (TAA)-induced fibrosis progression or during recovery. Therapeutic efficacy in biliary fibrosis was tested in BALB/c...
January 10, 2017: Gut
https://www.readbyqxmd.com/read/28069510/species-specific-effects-of-aortic-valve-decellularization
#5
Mitchell C VeDepo, Eric E Buse, Rachael W Quinn, Todd Williams, Michael S Detamore, Richard A Hopkins, Gabriel L Converse
: Decellularized heart valves have great potential as a stand-alone valve replacement or as a scaffold for tissue engineering heart valves. Before decellularized valves can be widely used clinically, regulatory standards require pre-clinical testing in an animal model, often sheep. Numerous decellularization protocols have been applied to both human and ovine valves; however, the ways in which a specific process may affect valves of these species differently have not been reported. In the current study, the comparative effects of decellularization were evaluated for human and ovine aortic valves by measuring mechanical and biochemical properties...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28068870/induced-remodeling-of-porcine-tendons-to-human-anterior-cruciate-ligaments-by-%C3%AE-gal-epitope-removal-and-partial-crosslinking
#6
Kevin Robert Stone, Ann Walgenbach, Uri Galili
This review describes a novel method developed for processing porcine tendon and other ligament implants which enables in situ remodeling into autologous ligaments in humans. The method differs from methods using extracellular matrices (ECM) which provide post-operative ortho-biologic support (i.e. augmentation grafts) for healing of injured ligaments, in that the porcine bone-patellar-tendon-bone itself serves as the graft replacing ruptured anterior cruciate ligament (ACL). The method allows for gradual remodeling of porcine tendon into autologous human ACL while maintaining the biomechanical integrity...
January 9, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28055099/evaluation-of-therapeutic-tissue-crosslinking-txl-for-myopia-using-second-harmonic-generation-signal-microscopy-in-rabbit-sclera
#7
Mariya Zyablitskaya, Anna Takaoka, Emilia L Munteanu, Takayuki Nagasaki, Stephen L Trokel, David C Paik
Purpose: Second harmonic generation signals (SHG) are emitted preferentially from collagenous tissue structures and have been used to evaluate photochemically-induced (CXL) crosslinking changes in the cornea. Since therapeutic tissue crosslinking (TXL) using sodium hydroxymethylglycinate (SMG) of the sclera is a potential treatment for high myopia, we explored the use of SHG microscopy to evaluate the effects. Methods: Single sub-Tenon's (sT) injections (400 μL) using SMG (40-400 mM) were made at the equatorial 12 o'clock position of the right eye of cadaveric rabbit heads (n = 16 pairs)...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28055060/optical-coherence-elastography-for-evaluating-customized-riboflavin-uv-a-corneal-collagen-crosslinking
#8
Manmohan Singh, Jiasong Li, Srilatha Vantipalli, Zhaolong Han, Kirill V Larin, Michael D Twa
No abstract text is available yet for this article.
September 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28054696/unraveling-capillary-interaction-and-viscoelastic-response-in-atomic-force-microscopy-of-hydrated-collagen-fibrils
#9
Manuel R Uhlig, Robert Magerle
The mechanical properties of collagen fibrils depend on the amount and the distribution of water molecules within the fibrils. Here, we use atomic force microscopy (AFM) to study the effect of hydration on the viscoelastic properties of reconstituted type I collagen fibrils in air with controlled relative humidity. With the same AFM tip, we investigate the same area of a collagen fibril with two different force spectroscopy methods: force-distance (FD) and amplitude-phase-distance (APD) measurements. This allows us to separate the contributions of the fibril's viscoelastic response and the capillary force to the tip-sample interaction...
January 5, 2017: Nanoscale
https://www.readbyqxmd.com/read/28041746/evaluation-of-cultured-human-dermal-and-dermo-epidermal-substitutes-focusing-on-extracellular-matrix-components-comparison-of-protein-and-rna-analysis
#10
Corien Oostendorp, Sarah Meyer, Monia Sobrio, Joyce van Arendonk, Ernst Reichmann, Willeke F Daamen, Toin H van Kuppevelt
Treatment of full-thickness skin defects with split-thickness skin grafts is generally associated with contraction and scar formation and cellular skin substitutes have been developed to improve skin regeneration. The evaluation of cultured skin substitutes is generally based on qualitative parameters focusing on histology. In this study we focused on quantitative evaluation to provide a template for comparison of human bio-engineered skin substitutes between clinical and/or research centers, and to supplement histological data...
December 29, 2016: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28040611/factors-affecting-the-mechanical-behavior-of-collagen-hydrogels-for-skin-tissue-engineering
#11
Marco Pensalfini, Alexander E Ehret, Silvia Stüdeli, Daniela Marino, Andres Kaech, Ernst Reichmann, Edoardo Mazza
The effect of the production factors yielding a functional dermal substitute was investigated by means of monotonic and cyclic uniaxial tensile tests, as well as electron microscopy visualizations. The role of (i) plastic compression, (ii) product incubation, and (iii) cell permanence in the collagenous matrix in order to achieve a skin-like behavior were characterized in terms of material and structural stiffness, in-plane kinematics, and cyclic response, as well as pore size and network density. The plastic compression resulted in a denser and stiffer material, while no corresponding change was observed in the behavior of the entire structure...
December 9, 2016: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28039063/encapsulation-of-primary-salivary-gland-cells-in-enzymatically-degradable-poly-ethylene-glycol-hydrogels-promotes-acinar-cell-characteristics
#12
Andrew D Shubin, Timothy J Felong, Brittany E Schutrum, Debria S L Joe, Catherine E Ovitt, Danielle S W Benoit
: Radiation therapy for head and neck cancers leads to permanent xerostomia due to the loss of secretory acinar cells in the salivary glands. Regenerative treatments utilizing primary submandibular gland (SMG) cells show modest improvements in salivary secretory function, but there is limited evidence of salivary gland regeneration. We have recently shown that poly(ethylene glycol) (PEG) hydrogels can support the survival and proliferation of SMG cells as multicellular spheres in vitro...
December 27, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/28029125/ameliorative-effect-of-curcumin-encapsulated-hyaluronic-acid-pla-nanoparticles-on-thioacetamide-induced-murine-hepatic-fibrosis
#13
Yu-Nong Chen, Shih-Lan Hsu, Ming-Yuan Liao, Yi-Ting Liu, Chien-Hung Lai, Ji-Feng Chen, Mai-Huong Thi Nguyen, Yung-Hsiang Su, Shang-Ting Chen, Li-Chen Wu
In this study, we developed curcumin-encapsulated hyaluronic acid-polylactide nanoparticles (CEHPNPs) to be used for liver fibrosis amelioration. CD44, the hyaluronic acid (HA) receptor, is upregulated on the surface of cancer cells and on activated hepatic stellate cells (aHSCs) rather than normal cells. CEHPNPs could bind to CD44 and be internalized effectively through endocytosis to release curcumin, a poor water-soluble liver protective agent. Thus, CEHPNPs were potentially not only improving drug efficiency, but also targeting aHSCs...
December 24, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28025887/influence-of-crosslinkers-on-the-in-vitro-chondrogenesis-of-mesenchymal-stem-cells-in-hyaluronic-acid-hydrogels
#14
Panita Maturavongsadit, Xiangdong Bi, Kamolrat Metavarayuth, Jittima Amie Luckanagul, Qian Wang
This study aims to investigate the effect of the structures of crosslinkers on the in vitro chondrogenic differentiation of bone mesenchymal stem cells (BMSCs) in hyaluronic acid (HA)-based hydrogels. The hydrogels were prepared by the covalent crosslinking of methacrylated HA with different types of thiol-tailored molecules, including dithiothreitol (DTT), 4-arm polyethylene glycol (PEG), and multi-arm polyamidoamine (PAMAM) dendrimer using thiol-ene "click" chemistry. The microstructure, mechanical properties, diffusivity and degradation rates of the resultant hydrogels were controlled by the structural feature of different crosslinkers...
December 27, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28017764/injectable-photo-crosslinked-enhanced-double-network-hydrogels-from-modified-sodium-alginate-and-gelatin
#15
Liu Yuan, Yu Wu, Qi-Sheng Gu, Hany El-Hamshary, Mohamed El-Newehy, Xiumei Mo
Recently, photocrosslinked hydrogels have attracted more and more attention in biomedical applications. In this study, a serials of injectable hydrogels were fabricated from aldehyde methacrylate sodium alginate and amino gelatin (AMSA/AG) using a two-step process. Here, sodium alginate, a kind of natural polysaccharide, was modified by oxidizer to form aldehyde sodium alginate (ASA), and methacrylate groups were further grafted on the main chain of ASA. Gelatin, the denatured form of collagen, was modified with ethylenediamine (ED) to graft more amino groups...
December 23, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28013007/effect-of-chromium-iii-gallate-complex-on-stabilization-of-collagen
#16
Dhanya Jaikumar, Babu Baskaran, V G Vaidyanathan
To improve the stability of the collagen, here we studied the interaction of chromium(III) polyphenolic complex, [Cr(GA)2] (GA: Gallic acid) with collagen using various spectroscopic techniques. Circular dichroism studies show that the [Cr(GA)2] and gallic acid did not induce any structural perturbations on the triple helix of the collagen. Both differential scanning calorimetric(DSC) data and micro-shrinkage temperature studies showed that [Cr(GA)2] stabilized the collagen by 6±1°C compared to gallic acid...
December 21, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28009397/corneal-collagen-crosslinking-for-corneal-ectasias-a-review
#17
David P S O'Brart
PURPOSE: To review the published literature on corneal collagen crosslinking (CXL). METHODS: Importance has been placed on seminal publications, systemic reviews, meta-analyses, and randomized controlled clinical trials. Where such evidence was not available, cohort studies, case-controlled studies, and case series with follow-up greater than 12 months were examined. RESULTS: Corneal collagen crosslinking with riboflavin and ultraviolet A (UVA) 370 nm radiation appears to be capable of arresting the progression of ectatic corneal disorders, with most studies reporting significant improvements in visual, keratometric, and topographic measurements...
December 6, 2016: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28008290/in-situ-gelation-properties-of-a-collagen-genipin-sol-with-a-potential-for-the-treatment-of-gastrointestinal-ulcers
#18
Takefumi Narita, Shunji Yunoki, Yoshimi Ohyabu, Naohisa Yahagi, Toshio Uraoka
We investigated the potential of collagen-genipin sols as biomaterials for treating artificial ulcers following endoscopic submucosal dissection. Collagen sol viscosity increased with condensation, allowing retention on tilted ulcers before gelation and resulting in collagen gel deposition on whole ulcers. The 1.44% collagen sols containing genipin as a crosslinker retained sol fluidity at 23°C for >20 min, facilitating endoscopic use. Collagen sols formed gel depositions on artificial ulcers in response to body temperature, and high temperature responsiveness of gelation because of increased neutral phosphate buffer concentration allowed for thick gel deposition on tilted ulcers...
2016: Medical Devices: Evidence and Research
https://www.readbyqxmd.com/read/28007116/accelerated-corneal-collagen-crosslinking-technique-efficacy-safety-and-applications
#19
REVIEW
Carla S Medeiros, Natalia T Giacomin, Renata L Bueno, Ramon C Ghanem, Haroldo V Moraes, Marcony R Santhiago
: Corneal collagen crosslinking (CXL) is an approach used to increase the biomechanical stability of the stromal tissue. Over the past 10 years, it has been used to halt the progression of ectatic diseases. According to the photochemical law of reciprocity, the same photochemical effect is achieved with reduced illumination time and correspondingly increased irradiation intensity. Several new CXL devices offer high ultraviolet-A irradiation intensity with different time settings. The main purpose of this review was to discuss the current use of different protocols of accelerated CXL and compare the efficacy and safety of accelerated CXL with the efficacy and safety of the established conventional method...
December 2016: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28004245/repairing-the-ruptured-annular-fibrosus-by-using-type-i-collagen-combined-with-citric-acid-edc-and-nhs-an-in-vivo-study
#20
Yan Wang, Xiaoting Wang, Jin Shang, Huan Liu, Yi Yuan, Yu Guo, Bo Huang, Yue Zhou
OBJECTIVES: To explore the effect of citric acid (CA)-1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/N-hydroxysuccinimide (NHS) collagen gel on repairing annular defects. METHODS: Type I collagen was extracted from the rat-tail tendon and crosslinked with CA at different mass ratio using EDC and NHS as crosslinking reagents to prepare four kinds of collagen gels. Forty-eight adult SD rats were divided into first sham group (n = 8), second group (n = 10) which was punctured and injected with CA-EDC/NHS collagen gel, third group (n = 10) which was punctured and injected with CA-EDC/NHS collagen gel, fourth group (n = 10) which was punctured and injected with EDC/NHS collagen gel, and fifth group (n = 10) which was punctured and untreated...
December 21, 2016: European Spine Journal
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