keyword
MENU ▼
Read by QxMD icon Read
search

Collagen crosslinking

keyword
https://www.readbyqxmd.com/read/28813714/effects-of-riboflavin-and-ultraviolet-illumination-on-the-biomechanical-properties-of-conjunctiva
#1
Harrison Man Hin Chan, Bonnie Nga Kwan Choy, Jimmy Shiu Ming Lai
PURPOSE: Our experiment evaluated the biomechanical effects of riboflavin and ultraviolet A illumination, in terms of covalent bonding, permeability, and indentation rupture force, on excised cattle conjunctiva. MATERIALS AND METHODS: Cattle conjunctiva was extracted and divided into the treatment group, which was soaked in riboflavin, followed by ultraviolet light illumination, and the control group. Samples were subsequently analysed on a Fourier transform infrared spectrum test to identify the presence of amide bonds, a permeability test to assess the diffusion of methylene blue across conjunctiva, and an indentation rupture force test...
August 17, 2017: Ophthalmic Research
https://www.readbyqxmd.com/read/28811773/transepithelial-corneal-crosslinking-in-treatment-of-progressive-keratoconus-12-months-clinical-results
#2
Bushra Akbar, Rana Intisar-Ul-Haq, Mazhar Ishaq, Sabahat Arzoo, Kashif Siddique
OBJECTIVE: The purpose of this study was to evaluate the safety and efficacy of transepithelial corneal collagen cross linking (TE-CXL) with modified riboflavin and accelerated UVA irradiance in thin corneas with pachymetry less than 400 microns at thinnest point, untreatable by epithelium off corneal collagen cross linking (CXL) in adult Pakistani population with progressive keratoconus. METHODS: This quasi experimental study included twenty six eyes of 26 patients with progressive keratoconus who underwent accelerated transepithelial CXL in Armed forced institute of ophthalmology with 12 months follow up...
May 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28811075/regulatory-t-cell-deficient-scurfy-mice-exhibit-a-th2-m2-like-inflammatory-response-in-the-skin
#3
Stefanie Haeberle, Verena Raker, Jessica Haub, Yong O Kim, Shih-Yen Weng, Osman K Yilmaz, Alexander Enk, Kerstin Steinbrink, Detlef Schuppan, Eva N Hadaschik
BACKGROUND: Scurfy mice have a functional defect in regulatory T cells (Treg), which leads to lethal multi-organ inflammation. The missing Treg function results in uncontrolled autoimmune cellular and humoral inflammatory responses. We and others have previously shown that during the course of disease scurfy mice develop severe skin inflammation and autoantibodies including anti-nuclear autoantibodies (ANA). OBJECTIVE: Autoimmune skin inflammation and ANA are hallmarks for the diagnosis of autoimmune connective tissue diseases; therefore we analyzed scurfy mice for typical signs of these diseases...
July 13, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28808811/construction-and-biocompatibility-of-a-thin-type-i-ii-collagen-composite-scaffold
#4
Long Han, Zhong-Wen Zhang, Bo-He Wang, Zhen-Kun Wen
Articular cartilage injury is a common type of damage observed in clinical practice. A matrix-induced autologous chondrocyte implant was developed to repair articular cartilage as an advancement on the autologous chondrocyte implant procedure. Here, we establish a thin double layer of collagen as a novel and effective bioscaffold for the regeneration of cartilaginous lesions. We created a collagen membrane with double layers using a cover slip, a cover slip, and the collagen was then freeze-dried under vacuum...
August 14, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28806419/effects-of-short-term-dry-immersion-on-bone-remodeling-markers-insulin-and-adipokines
#5
Marie-Thérèse Linossier, Liubov E Amirova, Mireille Thomas, Myriam Normand, Marie-Pierre Bareille, Guillemette Gauquelin-Koch, Arnaud Beck, Marie-Claude Costes-Salon, Christine Bonneau, Claude Gharib, Marc-Antoine Custaud, Laurence Vico
BACKGROUND: Dry immersion (DI), a ground-based model of microgravity previously used in Russia, has been recently implemented in France. The aim of this study was to analyze early events in a short-term DI model in which all conditions are met to investigate who is first challenged from osteo- or adipo-kines and to what extent they are associated to insulin-regulating hormones. METHODS: Twelve healthy men were submitted to a 3-day DI. Fasting blood was collected during pre-immersion phase for the determination of the baseline data collection (BDC), daily during DI (DI24h, DI48H and DI72h), then after recovery (R+3h and R+24h)...
2017: PloS One
https://www.readbyqxmd.com/read/28804996/in-vitro-evaluation-of-3d-bioprinted-tri-polymer-network-scaffolds-for-bone-tissue-regeneration
#6
Stephanie T Bendtsen, Mei Wei
In vitro evaluations provide vital information on the ability of tissue engineered scaffolds to support cell life and promote natural physiological behaviors in culture. Such assessments are necessary to conduct before implementation of the scaffolds for tissue healing in vivo. The scaffold extracellular matrix must provide the biochemical and mechanical cues necessary to promote cellular attachment, migration and proliferation before differentiation and new tissue deposition can occur. In this study, an in vitro evaluation was conducted to assess the ability of scaffolds 3D printed with a previously developed alginate-polyvinyl alcohol-hydroxyapatite formulation to promote proliferation of encapsulated MC3T3 cells...
August 14, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28800626/targeting-lysyl-oxidase-reduces-peritoneal-fibrosis
#7
Christopher R Harlow, Xuan Wu, Marielle van Deemter, Fiona Gardiner, Craig Poland, Rebecca Green, Sana Sarvi, Pamela Brown, Karl E Kadler, Yinhui Lu, J Ian Mason, Hilary O D Critchley, Stephen G Hillier
BACKGROUND: Abdominal surgery and disease cause persistent abdominal adhesions, pelvic pain, infertility and occasionally, bowel obstruction. Current treatments are ineffective and the aetiology is unclear, although excessive collagen deposition is a consistent feature. Lysyl oxidase (Lox) is a key enzyme required for crosslinking and deposition of insoluble collagen, so we investigated whether targeting Lox might be an approach to reduce abdominal adhesions. METHODS: Female C57Bl/6 mice were treated intraperitoneally with multiwalled carbon nanotubes (NT) to induce fibrosis, together with chemical (ß-aminoproprionitrile-BAPN) or miRNA Lox inhibitors, progesterone or dexamethasone...
2017: PloS One
https://www.readbyqxmd.com/read/28795951/gelma-collagen-blends-enable-drop-on-demand-3d-printablility-and-promote-angiogenesis
#8
Henrike Stratesteffen, Marius Johannes Köpf, Franziska Kreimendahl, Andreas Blaeser, Stefan Jockenhoevel, Horst Fischer
Effective vascularization is crucial for three dimensional (3D) printed hydrogel-cell constructs to efficiently supply cells with oxygen and nutrients. Till date, several hydrogel blends have been developed that allow the in vitro formation of a capillary-like network within the gels but comparatively less effort has been made to improve the suitability of the materials for a 3D bioprinting process. Therefore, we hypothesize that tailored hydrogel blends of photo-crosslinkable gelatin and type I collagen exhibit favorable 3D drop-on-demand printing characteristics in terms of rheological and mechanical properties and that further capillary-like network formation can be induced by co-culturing human umbilical vein endothelial cells (HUVEC) and human mesenchymal stem cells (hMSC) within the proposed blends...
August 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28791838/collagenous-scaffolds-supplemented-with-hyaluronic-acid-and-chondroitin-sulfate-used-for-wound-fibroblast-and-embryonic-nerve-cell-culture
#9
Jacek Drobnik, Krystyna Pietrucha, Lucyna Piera, Jacek Szymański, Alicja Szczepanowska
BACKGROUND: Tissue engineering is a strategy aimed at improving the regeneration of injured tissues. OBJECTIVES: The aim of the present study was to determine whether a tri-copolymer composed of crosslinked collagen, chondroitin sulfate and hyaluronic acid (Col + CS + HA) provides a better environment for fibroblast and embryonic nerve cell culture than a collagenous scaffold (Col). MATERIAL AND METHODS: The porosity of each of the matrices was characterized with a scanning electron microscope...
March 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28782046/circulating-loxl2-levels-reflect-severity-of-intestinal-fibrosis-and-galt-cd4-t-lymphocyte-depletion-in-treated-hiv-infection
#10
Sophie Seang, Anoma Somasunderam, Maitreyee Nigalye, Ma Somsouk, Timoty W Schacker, Joyce L Sanchez, Peter W Hunt, Netanya S Utay, Jordan E Lake
BACKGROUND: Incomplete immune reconstitution may occur despite successful antiretroviral therapy (ART). Gut-associated lymphoid tissue (GALT) fibrosis may contribute via local CD4(+) T lymphocyte depletion, intestinal barrier disruption, microbial translocation, and immune activation. METHODS: In a cross-sectional analysis, we measured circulating fibrosis biomarker levels on cryopreserved plasma from adult HIV-infected (HIV+) SCOPE study participants on suppressive ART who also had fibrosis quantification on recto-sigmoid biopsies...
2017: Pathogens & Immunity
https://www.readbyqxmd.com/read/28780464/nile-tilapia-skin-collagen-sponge-modified-with-chemical-cross-linkers-as-a-biomedical-hemostatic-material
#11
Leilei Sun, Bafang Li, Dandan Jiang, Hu Hou
Nile tilapia skin collagen sponges were fabricated by freeze-drying technology and modified with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide in the presence of N-hydroxysuccinimide (EDC/NHS), genipin+PBS, genipin+ethanol, tea polyphenol (TP), nordihydroguaiaretic acid (NDGA) and diphenyl phosphoryl azide (DPPA). Physicochemical and biological properties, micromorphology and compatibility before and after modification were investigated to evaluate collagen sponge as a hemostatic biomedical material. The mechanical property of collagen sponges strengthened after cross-linking...
July 26, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28780254/modeling-the-response-of-exogenously-crosslinked-tissue-to-cyclic-loading-the-effects-of-permanent-set
#12
Will Zhang, Michael S Sacks
Bioprosthetic heart valves (BHVs), fabricated from exogenously crosslinked collagenous tissues, remain the most popular heart valve replacement design. However, the life span of BHVs remains limited to 10-15 years, in part because the mechanisms that underlie BHV failure remain poorly understood. Experimental evidence indicates that BHVs undergo significant changes in geometry with in vivo operation, which lead to stress concentrations that can have significant impact on structural damage. These changes do not appear to be due to plastic deformation, as the leaflets only deform in the elastic regime...
July 11, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28775095/transglutaminase-2-in-pulmonary-and-cardiac-tissue-remodeling-in-experimental-pulmonary-hypertension
#13
Krishna C Penumatsa, Deniz Toksoz, Rod R Warburton, Mousa Kharnaf, Ioana R Preston, Navin Kumar Kapur, Chaitan Khosla, Nicholas S Hill, Barry L Fanburg
Tissue matrix remodeling and fibrosis leading to loss of pulmonary arterial and right ventricular compliance are important features of both experimental and clinical pulmonary hypertension (PH). We have previously reported that transglutaminase 2 (TG2) is involved in PH development while others have shown it to be a crosslinking enzyme that participates in remodeling of extracellular matrix in fibrotic diseases in general. In the present studies, we used a mouse model of experimental PH (Sugen 5416 and hypoxia; SuHypoxia) and cultured primary human cardiac and pulmonary artery adventitial fibroblasts to evaluate the relationship of TG2 to the processes of fibrosis, protein crosslinking, extracellular matrix collagen accumulation and fibroblast to myofibroblast transformation...
August 3, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28763562/chondroitin-sulfate-based-biocompatible-crosslinker-restores-corneal-mechanics-and-collagen-alignment
#14
Xiaokun Wang, Shoumyo Majumdar, Garret Ma, Jeeyeon Sohn, Samuel C Yiu, Walter Stark, Awad Al-Qarni, Deepak P Edward, Jennifer H Elisseeff
Purpose: To evaluate the crosslinking effect of functionalized chondroitin sulfate (CS) in an ex vivo rabbit cornea model. Methods: Chondroitin sulfate molecules were chemically modified with the N-hydroxysuccinimide (NHS) group. Enucleated rabbit eyes were crosslinked with 2, 5, or 10 mg/mL CS-NHS solution for 30 or 60 minutes. The CS-NHS penetration, corneal swelling ratio, Young's modulus, and ultrastructure of the crosslinked corneas were characterized. In addition, rabbit corneas were further treated with a collagenase-chondroitinase solution to create an ex vivo keratoconus (KC)-like model...
August 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28751073/efficacy-of-new-natural-biomodification-agents-from-anacardiaceae-extracts-on-dentin-collagen-cross-linking
#15
M A Moreira, N O Souza, R S Sousa, D Q Freitas, M V Lemos, D M De Paula, F J N Maia, D Lomonaco, S E Mazzetto, V P Feitosa
OBJECTIVES: Several polyphenols from renewable sources were surveyed for dentin biomodification. However, phenols from cashew nut shell liquid (CNSL, Anacardium occidentale) and from Aroeira (Myracrodruon urundeuva) extract have never been evaluated. The present investigation aimed to compare the dentin collagen crosslinking (biomodification) effectiveness of polyphenols from Aroeira stem bark extract, proanthocyanidins (PACs) from grape-seed extract (Vitis vinifera), cardol and cardanol from CNSL after clinically relevant treatment for one minute...
July 24, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28747621/evaluation-of-biomechanical-changes-in-myopia-patients-with-unsatisfactory-corneas-after-femto-second-laser-in-situ-keratomileusis-fs-lasik-concurrent-with-accelerated-corneal-collagen-cross-linking-using-corvis-st-two-year-follow-up-results
#16
Weiwei Xu, Ye Tao, Liqiang Wang, Yifei Huang
BACKGROUND Some myopia patients with unsatisfactory corneas consider corneal refractive surgery for different reasons. Accelerated corneal collagen crosslinking (ACXL) is an effective method to enhance the resistance of the cornea. The present investigation was designed to evaluate the changes of biomechanical properties in patients with myopia and thin corneas after femtosecond-laser in situ keratomileusis (FS-LASIK) concurrent with ACXL. MATERIAL AND METHODS A prospective study was designed. A total of 22 eyes of 11 myopia astigmatism patients with unsatisfactory corneas were enrolled...
July 27, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28743854/-new-methods-for-the-evaluation-of-bone-quality-bone-anabolic-agents-and-bone-quality
#17
Norio Yamamoto, Hiroyuki Tsuchiya
Teriparatide(TPTD)products that can be used clinically in Japan include a daily subcutaneous injection form produced by genetic engineering and a weekly subcutaneous injectable TPTD acetate form produced by chemical synthesis. Published reports indicate that both forms exhibit excellent antifracture efficacy, and as the only anabolic agents that promote osteogenesis, TPTD products now occupy a prominent position. However, the two forms differ considerably, not only in frequency of administration, but also in mechanism of action...
2017: Clinical Calcium
https://www.readbyqxmd.com/read/28741862/in-vivo-comparison-of-jellyfish-and-bovine-collagen-sponges-as-prototype-medical-devices
#18
Jonathan P Widdowson, Alex J Picton, Valerie Vince, Chris J Wright, Andrew Mearns-Spragg
Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vectors, both of which could be transmitted to humans. Here we compare collagen implantable sponges derived for the first time from the Rhizostoma pulmo jellyfish. A further novelty for the research was that there was a comparison for sponges that were either uncrosslinked or crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and an assessment on how this affected resorption, as well as their biocompatibility compared to bovine type I collagen sponges...
July 25, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28728606/levels-of-serum-biomarkers-from-a-two-year-multicentre-trial-are-associated-with-treatment-response-on-knee-osteoarthritis-cartilage-loss-as-assessed-by-magnetic-resonance-imaging-an-exploratory-study
#19
Johanne Martel-Pelletier, Jean-Pierre Raynauld, François Mineau, François Abram, Patrice Paiement, Philippe Delorme, Jean-Pierre Pelletier
BACKGROUND: There is an obvious need to identify biomarkers that could predict patient response to an osteoarthritis (OA) treatment. This post hoc study explored in a 2-year randomized controlled trial in patients with knee OA, the likelihood of some serum biomarkers to be associated with a better response to chondroitin sulfate in reducing cartilage volume loss. METHODS: Eight biomarkers were studied: hyaluronic acid (HA), C reactive protein (CRP), adipsin, leptin, N-terminal propeptide of collagen IIα (PIIANP), C-terminal crosslinked telopeptide of type I collagen (CTX-1), matrix metalloproteinase-1 (MMP-1), and MMP-3...
July 20, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28726576/recent-developments-in-thiolated-polymeric-hydrogels-for-tissue-engineering-applications
#20
Mani Gajendiran, Jae-Sung Rhee, Kyobum Kim
This review focuses on the recent strategy in the preparation of thiolated polymers and fabrication of their hydrogel matrices. The mechanism involved in the synthesis of thiolated polymers and fabrication of thiolated polymer hydrogels is exemplified with suitable schematic representations reported in the recent literature. The 2-iminothiolane namely "Traut's reagent" has been widely employed for effectively thiolating the natural polymers such as collagen and gelatin which contain free amino group in their back bone...
July 20, 2017: Tissue Engineering. Part B, Reviews
keyword
keyword
21852
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"