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Collagen crosslinking

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https://www.readbyqxmd.com/read/29318768/use-of-instrumented-indentation-for-determination-of-mechanical-properties-of-human-cornea-after-uva-crosslinking
#1
Jiri Nohava, Michael Swain, Stefan J Lang, Philip Maier, Sonja Heinzelmann, Thomas Reinhard, Philipp Eberwein
UVA-crosslinking is used for treatment of corneal diseases such as keratoconus in order to stabilize the corneal tissue by crosslinking of the collagen fibers. It has been shown that the crosslinking treatment leads to a stiffening of the central corneal tissue. However, knowledge of lateral extent of the corneal stiffening as well as a systematic study of the mechanical response of human cornea is still missing. In our study we measured the stiffness (elastic modulus) of the anterior surface of healthy and crosslinked human corneas by instrumented indentation using a spherical indenter...
January 10, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29310777/basement-membrane-collagen-iv-isolation-of-functional-domains
#2
Sergei P Boudko, Neonila Danylevych, Billy G Hudson, Vadim K Pedchenko
Collagen IV is a major constituent of basement membranes, specialized form of extracellular matrix that provides a mechanical support for tissues, serves as a polyvalent ligand for cell adhesion receptors and as a scaffold for other proteins, and plays a key role in tissue genesis, differentiation, homeostasis, and remodeling. Collagen IV underlies the pathogenesis of several human disorders including Goodpasture's disease, Alport's syndrome, diabetic nephropathy, angiopathy, and porencephaly. While the isolation of the collagen IV molecules from tissues is an ultimate prerequisite for structural and functional studies, it has been always hampered by the protein insolubility due to extensive intermolecular crosslinking and noncovalent associations with other components of basement membranes...
2018: Methods in Cell Biology
https://www.readbyqxmd.com/read/29309816/lysyl-oxidase-in-cancer-inhibition-and-metastasis
#3
Kathryn A Johnston, Karlo M Lopez
Lysyl oxidase is an extracellular matrix, copper - dependent amine oxidase that catalyzes a key enzymatic step in the crosslinking of collagen and elastin. The enzyme is synthesized as a propeptide that is cleaved by procollagen - C - proteinase into two distinct parts: the mature form and the LOX propeptide. The mature enzyme plays a key role in modifying the extracellular matrix and as a result has been implicated in playing a role in the formation of cancer "niches" where tumors will develop and eventually metastasize...
January 5, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29307874/tyrosinase-doped-bioink-for-3d-bioprinting-of-living-skin-constructs
#4
Hong Bo Zhang, Shi Yong, Xing Tianlong, Yin Ruixue, Yang Shimo, Jie Wei, Wenjun Zhang
3D Bioprinting is an emerging technology to fabricate 3D living constructs and it has shown a great promise in tissue engineering. Bioinks are scaffold materials mixed with cells used by 3D bioprinting to form a required cell-laden structure. In this paper, a novel bioink made of Gelatin Methacrylamide (GelMA) and Collagen (Col) doped with Tyrosinase (Ty) is presented for 3D bioprinting of living skin tissues. Ty has dual functions: one to serve as an essential bioactive compound in the skin regeneration process and the other to serve as enzyme to facilitate the crosslink of Col and GelMA...
January 8, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29305778/advanced-glycation-end-products-as-a-source-of-artifacts-in-immunoenzymatic-methods
#5
Aleksandra Kuzan, A Chwiłkowska, K Maksymowicz, A Szydełko-Bronowicka, K Stach, C Pezowicz, A Gamian
The most abundant proteins in the arteries are those of extracellular matrix, ie. collagen and elastin. Due to their long half-lifes these proteins have an increased chance to undergo glycation. The aim of this study was to determine relationship between the content of the main extracellular matrix proteins and the advanced glycation end products (AGEs) in arteries. In this study 103 fragments of aorta were analyzed by ELISA and immunobloting for the content of collagens type I, III and IV and elastin and the content of advanced glycation end-products (AGE)...
January 5, 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29285987/anticancer-efficacy-of-tannic-acid-is-dependent-on-the-stiffness-of-the-underlying-matrix
#6
Christopher J Bridgeman, Thuy-Uyen Nguyen, Vipuil Kishore
Tannic acid (TA) has been previously shown to have anticancer potential for breast cancer but its effects on melanoma have not yet been investigated. Similarly, stiffness of the tumor microenvironment is known to have a profound effect on breast cancer metastasis, but little is known about its role on melanoma. The goal of the current study is to investigate the synergistic effects of TA and matrix stiffness on melanoma progression. A375 melanoma cells with metastatic potential were cultured on TA crosslinked uncompacted (soft) and electrochemically compacted (stiff) collagen gels and the effects of TA on gel morphology, mechanical properties, and cellular response (i...
December 29, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29282020/efficacy-and-safety-of-transepithelial-corneal-collagen-crosslinking-surgery-versus-standard-corneal-collagen-crosslinking-surgery-for-keratoconus-a-meta-analysis-of-randomized-controlled-trials
#7
Wenwei Li, Bin Wang
BACKGROUND: The aim of this study was to evaluate the efficacy and safety of transepithelial corneal collagen crosslinking (transepithelial CXL) versus standard corneal collagen crosslinking (epithelium-off CXL) on keratoconus. METHODS: Eligible studies were identified by systematically searching PubMed, the Cochrane Library and Embase. Topographic parameters, corrected distant visual acuity (CDVA), uncorrected distant visual acuity (UDVA), and corneal thickness (CT) were assessed by the pooled weighted mean differences (WMDs) of the change from baseline to the end of follow up...
December 28, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29281127/bone-tissue-collagen-maturity-and-mineral-content-increase-with-sustained-hyperglycemia-in-the-kk-ay-murine-model-of-type-2-diabetes
#8
Heather B Hunt, Jared C Pearl, David R Diaz, Karen B King, Eve Donnelly
Type 2 diabetes mellitus (T2DM) increases fracture risk for a given bone mineral density, which suggests that T2DM changes bone tissue properties independently of bone mass. In this study, we assessed the effects of hyperglycemia on bone tissue compositional properties, enzymatic collagen crosslinks, and advanced glycation endproducts (AGEs) in the KK-Ay murine model of T2DM using Fourier transform infrared imaging and high-performance liquid chromatography. Compared to KK-aa littermate controls (n = 8), proximal femoral bone tissue of KK-Ay mice (n = 14) exhibited increased collagen maturity, increased mineral content, and less heterogeneous mineral properties...
December 27, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29279675/the-effect-of-two-cross-linking-agents-on-dentin-bond-strength-of-resin-modified-glass-ionomer
#9
Fereshteh Shafiei, Maryam Firouzmandi, Moona Zamanpour
Objectives: The hybrid layer at the interface of resin-modified glass ionomer cements and dentin is prone to degradation by endogenous matrix metalloproteinases. We aimed to investigate the effect of two types of collagen crosslinkers, carbodiimide and proanthocyanidin (PA), on immediate and medium-term bond strength of a resin-modified glass ionomer to dentin. Materials and Methods: Seventy-two molars were flattened on the occlusal surface to expose dentin. The specimens were divided into control, 1-ethyl-3-(3-dimethylaminopropy) carbodiimide hydrochloride (EDC), and PA groups (n = 24)...
October 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/29278504/porous-gelatin-membrane-obtained-from-pickering-emulsions-stabilized-by-graphene-oxide
#10
Sakthivel Nagarajan, Dominique Abessolo Ondo, Sana Gassara, Mikhael Bechelany, Sebastien Balme, Philippe Miele, Narayana S Kalkura, Celine Pochat-Bohatier
This paper presents a novel procedure to prepare porous membranes from water-soluble polymers involving the formation of Pickering emulsion. Gelatin is a biodegradable biopolymer obtained by the partial hydrolysis of collagen. Biopolymer such as gelatin is capable to adsorb at oil/water interface and results to decrease interfacial energy. Hence, gelatin is widely employed as an alternate for synthetic surfactants to stabilize emulsions in food industry. However, high molecular weight gelatin leads to large emulsion droplets and poor emulsion stability...
December 26, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29275153/mechanical-stiffness-of-tmj-condylar-cartilage-increases-after-artificial-aging-by-ribose
#11
Fereshteh Mirahmadi, Jan Harm Koolstra, Frank Lobbezoo, G Harry van Lenthe, Samaneh Ghazanfari, Jessica Snabel, Reinout Stoop, Vincent Everts
OBJECTIVE: Aging is accompanied by a series of changes in mature tissues that influence their properties and functions. Collagen, as one of the main extracellular components of cartilage, becomes highly crosslinked during aging. In this study, the aim was to examine whether a correlation exists between collagen crosslinking induced by artificial aging and mechanical properties of the temporomandibular joint (TMJ) condyle. To evaluate this hypothesis, collagen crosslinks were induced using ribose incubation...
December 16, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29249023/cortical-fractal-analysis-and-collagen-crosslinks-content-in-femoral-neck-after-osteoporotic-fracture-in-postmenopausal-women-comparison-with-osteoarthritis
#12
Gustavo Davi Rabelo, Jean-Paul Roux, Nathalie Portero-Muzy, Evelyne Gineyts, Roland Chapurlat, Pascale Chavassieux
The femoral neck (FN) has been previously characterized by thinner cortices in osteoporotic fracture (HF) when compared to hip osteoarthritis (HOA). The purposes of this study were to complete the previous investigations on FNs from HF and HOA by analyzing the complexity of the cortical structure and to approach the intrinsic properties of cortical bone by assessing the collagen crosslink contents. FN samples were obtained during arthroplasty in 35 postmenopausal women (HF; n = 17; mean age 79 ± 2 years; HOA; n = 18; mean age 66 ± 2 years)...
December 16, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/29247535/the-role-of-extracellular-matrix-stiffness-in-megakaryocyte-development-and-function
#13
REVIEW
Orly Leiva, Catherine Leon, Seng Kah Ng, Pierre Mangin, Christian Gachet, Katya Ravid
The extracellular matrix (ECM) is a key acellular structure in constant remodeling to provide tissue cohesion and rigidity. Deregulation of the balance between matrix deposition, degradation and crosslinking results in fibrosis. Bone marrow fibrosis (BMF) is associated with several malignant and nonmalignant pathologies severely affecting blood cell production. BMF results from abnormal deposition of collagen fibers and enhanced lysyl oxidase-mediated ECM crosslinking within the marrow, thereby increasing marrow stiffness...
December 16, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/29242757/differential-gene-transcription-of-extracellular-matrix-components-in-response-to-in-vivo-corneal-crosslinking-cxl-in-rabbit-corneas
#14
Sabine Kling, Arthur Hammer, Emilio A Torres Netto, Farhad Hafezi
Purpose: We studied changes in gene transcription after corneal crosslinking (CXL) in the rabbit cornea in vivo and identified potential molecular signaling pathways. Methods: A total of 15 corneas of eight male New-Zealand-White rabbits were de-epithelialized and equally divided into five groups. Group 1 served as an untreated control. Groups 2 to 5 were soaked with 0.1% riboflavin for 20 minutes, which in Groups 3 to 5 was followed by UV-A irradiation at a fluence of 5...
December 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/29229438/pulsed-electromagnetic-fields-pemf-attenuate-changes-in-vertebral-bone-mass-architecture-and-strength-in-ovariectomized-mice
#15
Tao Lei, Zhuowen Liang, Feijiang Li, Chi Tang, Kangning Xie, Pan Wang, Xu Dong, Shuai Shan, Maogang Jiang, Qiaoling Xu, Erping Luo, Guanghao Shen
Pulsed electromagnetic fields (PEMF) has been investigated as a noninvasive alternative method to prevent bone loss for postmenopausal osteoporosis (OP), and the bone tissue involved in these studies are usually long bones such as femur and tibia in OP patients or rat models. However, few studies have investigated the effects of PEMF on the vertebral bone in mice with OP. This study aimed to investigate whether PEMF preserve lumbar vertebral bone mass, microarchitecture and strength in ovariectomized (OVX) mouse model of OP and its associated mechanisms...
December 8, 2017: Bone
https://www.readbyqxmd.com/read/29227501/chondrogenic-potential-of-macroporous-biodegradable-cryogels-based-on-synthetic-poly-%C3%AE-amino-acids
#16
T Sedlačík, O K Acar, H Studenovská, I Kotelnikov, J Kučka, Z Konečná, T Zikmund, J Kaiser, G T Kose, F Rypáček
In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(α-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-l-glutamine-stat-N5-(2-methacryloyl-oxy-ethyl)-l-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers...
December 11, 2017: Soft Matter
https://www.readbyqxmd.com/read/29225769/fabrication-of-low-cost-micropatterned-polydimethyl-siloxane-scaffolds-to-organise-cells-in-a-variety-of-two-dimensioanl-biomimetic-arrangements-for-lab-on-chip-culture-platforms
#17
Lidia Escutia-Guadarrama, Genaro Vázquez-Victorio, David Martínez-Pastor, Brenda Nieto-Rivera, Marcela Sosa-Garrocho, Marina Macías-Silva, Mathieu Hautefeuille
We present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surface treatment is to stabilise microcontact printing transfer of this natural extracellular matrix protein that usually wears out easily from poly-dimethylsiloxane, which is not suitable for biomimetic cell culture platforms and lab-on-chip applications...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29225278/physiochemical-properties-and-resorption-progress-of-porcine-skin-derived-collagen-membranes-in-vitro-and-in-vivo-analysis
#18
Yin-Zhe An, You-Kyoung Kim, Su-Min Lim, Yeong-Ku Heo, Mi-Kyung Kwon, Jae-Kook Cha, Jung-Seok Lee, Ui-Won Jung, Seong-Ho Choi
The aim of the present study was to evaluate the physiochemical properties and resorption progress of two cross-linked, porcine skin-derived collagen membranes and compare their features with those of a membrane without cross-linking (Bio-Gide® [BG], Geistlich Biomaterials, Wolhusen, Switzerland). Three porcine skin-derived collagen membranes, dehydrothermally (DHT) crosslinked (experimental), DHT and 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide (DHT/EDC) cross-linked (experimental) and BG were investigated for their morphology, enzyme resistance, and tensile strength in vitro and biodegradation in vivo...
December 8, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/29221639/nerve-growth-factor-released-from-collagen-scaffolds-protects-axotomized-cholinergic-neurons-of-the-basal-nucleus-of-meynert-in-organotypic-brain-slices
#19
Bettina M Foidl, Buket Ucar, Alina Schwarz, Ana L Rebelo, Abhay Pandit, Christian Humpel
BACKGROUND: Alzheimeŕs disease is accompanied by cell death of cholinergic neurons, resulting in cognitive impairment and memory loss. Nerve growth factor (NGF) is the most potent protein to support survival of cholinergic neurons. New METHOD: Organotypic brain slices of the basal nucleus of Meynert (nBM) are a valuable tool to study cell death of axotomized cholinergic neurons, as well as protective effects of NGF added into the medium. The aim of the present study is to use collagen scaffolds crosslinked with polyethylineglycole and load with NGF to target delivery of NGF to organotypic nBM brain slices...
December 5, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/29219951/comparative-study-of-long-term-outcomes-of-accelerated-and-conventional-collagen-crosslinking-for-progressive-keratoconus
#20
J J Males, D Viswanathan
PurposeTo compare the long-term outcomes of accelerated corneal collagen crosslinking (CXL) to conventional CXL for progressive keratoconus.Patients and methodsComparative clinical study of consecutive progressive keratoconic eyes that underwent either accelerated CXL (9 mW/cm2 ultraviolet A (UVA) light irradiance for 10 min) or conventional CXL (3 mW/cm2 UVA light irradiance for 30 min). Eyes with minimum 12 months' follow-up were included. Post-procedure changes in keratometry readings (Flat meridian: K1; steep meridian: K2), central corneal thickness (CCT), best spectacle-corrected visual acuity (BSCVA), and manifest refraction spherical equivalent (MRSE) were analysed...
December 8, 2017: Eye
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