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https://www.readbyqxmd.com/read/28324231/occurrence-and-virulence-properties-of-vibrio-and-salinivibrio-isolates-from-tropical-lagoons-of-the-southern-caribbean-sea
#1
Milagro Fernández-Delgado, Paula Suárez, Sandra Giner, Virginia Sanz, Jessy Peña, Damarys Sánchez, M Alexandra García-Amado
The Vibrionaceae are Gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. The aim of this research was to investigate the occurrence and virulence properties of Vibrio and Salinivibrio isolated from lagoons at Cuare Wildlife Refuge and Margarita Island in the southern Caribbean Sea. Water, plankton and oyster samples were collected during October 2011 and March 2012 and examined by specific PCR and culture methods...
March 21, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28323406/affinity-selection-mass-spectrometry-identifies-a-novel-antibacterial-rna-polymerase-inhibitor
#2
Scott S Walker, David Degen, Elliott Nickbarg, Donna Carr, Aileen Soriano, Mihir Baran Mandal, Ronald E Painter, Payal R Sheth, Li Xiao, Xinwei Sher, Nicholas Murgolo, Jing Su, David B Olsen, Richard H Ebright, Katherine Young
The growing prevalence of drug-resistant Gram-negative bacteria is a significant global threat to human health. Rifampicin, an RNA polymerase-targeting agent, is an important part of the antibacterial armamentarium; however the emergence of resistance requires that it be used against only certain infections and usually in combination with another antibiotic. While rifampicin has significant clinical limitations, it does show that bacterial RNA polymerase can be an effective target for antibacterial intervention...
March 21, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28319906/functional-characterization-of-gram-negative-bacteria-from-different-genera-as-multiplex-cadmium-biosensors
#3
Lara Bereza-Malcolm, Sanja Aracic, Ruban Kannan, Gülay Mann, Ashley E Franks
Widespread presence of cadmium in soil and water systems is a consequence of industrial and agricultural processes. Subsequent accumulation of cadmium in food and drinking water can result in accidental consumption of dangerous concentrations. As such, cadmium environmental contamination poses a significant threat to human health. Development of microbial biosensors, as a novel alternative method for in situ cadmium detection, may reduce human exposure by complementing traditional analytical methods. In this study, a multiplex cadmium biosensing construct was assembled by cloning a single-output cadmium biosensor element, cadRgfp, and a constitutively expressed mrfp1 onto a broad-host range vector...
March 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28319867/phototherapeutic-spectrum-expansion-through-synergistic-effect-of-mesoporous-silica-trio-nanohybrids-against-antibiotic-resistant-gram-negative-bacterium
#4
Yaswanth Kuthati, Ranjith Kumar Kankala, Prabhakar Busa, Shi-Xiang Lin, Jin-Pei Deng, Chung-Yuan Mou, Chia-Hung Lee
The extensive impact of antibiotic resistance has led to the exploration of new anti-bacterial modalities. We designed copper impregnated mesoporous silica nanoparticles (Cu-MSN) with immobilizing silver nanoparticles (SNPs) to apply photodynamic inactivation (PDI) of antibiotic-resistant E. coli. SNPs were decorated over the Cu-MSN surfaces by coordination of silver ions on diamine-functionalized Cu-MSN and further reduced to silver nanoparticles with formalin. We demonstrate that silver is capable of sensitizing the gram-negative bacteria E...
March 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28318629/machine-learning-algorithms-define-pathogen-specific-local-immune-fingerprints-in-peritoneal-dialysis-patients-with-bacterial-infections
#5
Jingjing Zhang, Ida M Friberg, Ann Kift-Morgan, Gita Parekh, Matt P Morgan, Anna Rita Liuzzi, Chan-Yu Lin, Kieron L Donovan, Chantal S Colmont, Peter H Morgan, Paul Davis, Ian Weeks, Donald J Fraser, Nicholas Topley, Matthias Eberl
The immune system has evolved to sense invading pathogens, control infection, and restore tissue integrity. Despite symptomatic variability in patients, unequivocal evidence that an individual's immune system distinguishes between different organisms and mounts an appropriate response is lacking. We here used a systematic approach to characterize responses to microbiologically well-defined infection in a total of 83 peritoneal dialysis patients on the day of presentation with acute peritonitis. A broad range of cellular and soluble parameters was determined in peritoneal effluents, covering the majority of local immune cells, inflammatory and regulatory cytokines and chemokines as well as tissue damage-related factors...
March 16, 2017: Kidney International
https://www.readbyqxmd.com/read/28317564/odontogenic-infections
#6
REVIEW
Orrett E Ogle
The pathogenesis of odontogenic infection is polymicrobial, consisting of various facultative and strict anaerobes. The dominant isolates are strictly anaerobic gram-negative rods and gram-positive cocci. The periapical infection is the most common form of odontogenic infection. Although odontogenic infections are usually confined to the alveolar ridge vicinity, they can spread into deep fascial spaces. Cavernous sinus thrombosis, brain abscess, airway obstruction, and mediastinitis are possible complications of dental infections...
April 2017: Dental Clinics of North America
https://www.readbyqxmd.com/read/28317027/the-type-a-and-type-b-polysaccharide-capsules-predominate-in-an-international-collection-of-invasive-kingella-kingae-isolates
#7
Eric A Porsch, Kimberly F Starr, Pablo Yagupsky, Joseph W St Geme
Kingella kingae is an encapsulated Gram-negative bacterium and an important etiology of osteoarticular infections in young children. A recent study examining a diverse collection of carrier and invasive K. kingae isolates from Israel revealed four distinct polysaccharide capsule types. In this study, to obtain a global view of K. kingae capsule type diversity, we examined an international collection of isolates using a multiplex PCR approach. The collection contained all four previously identified capsule types and no new capsule types...
March 2017: MSphere
https://www.readbyqxmd.com/read/28316932/bacterial-fauna-associating-with-chironomid-larvae-from-lakes-of-bengaluru-city-india-a-16s-rrna-gene-based-identification
#8
Ramprasad Kuncham, Thiyagarajan Sivaprakasam, R Puneeth Kumar, P Sreenath, Ravi Nayak, Tha Thayumanavan, Gopireddy V Subba Reddy
Chironomid larvae that inhabit in aquatic sediments play an important role as vector for bacterial pathogens. Its life cycle consists of four stages i.e. eggs, larvae, pupae and adult. In the present study we identified bacterial species associated with whole larvae of chironomids from 11 lake sediments of Bangalore region using 16s rRNA gene Sanger sequencing. We found that larvae from all lake sediments associated with bacterial species which include key pathogens. Totally we identified 65 bacterial isolates and obtained GenBank accession numbers (KX980423 - KX980487)...
June 2017: Genomics Data
https://www.readbyqxmd.com/read/28315976/tfoi-produced-by-tepidimonas-fonticaldi-pl17-a-moderate-thermophilic-bacterium-is-an-isoschizomer-of-msei
#9
Ravinder Kumar, Anil Kumar Pinnaka, Beena Krishnan
A moderately thermophilic Gram-negative bacterium isolated from the Polok hot spring, Sikkim, India, was identified as a strain (PL17) of Tepidimonas fonticaldi by 16S rDNA sequencing. T. fonticaldi PL17 produces a Type IIP restriction endonuclease; named TfoI. Restriction mapping, run-off sequencing of TfoI-digests of dsDNA fragments, and end compatibility of TfoI with NdeI confirmed that the enzyme recognizes and cleaves the sequence 5'-T^TAA-3', and is thus an isoschizomer of MseI. The TfoI restriction-modification genes in the T...
March 18, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28315912/an-imaging-target-tgf-%C3%AE-1-for-hepatocellular-carcinoma-in-mice
#10
Yiqiu Zhang, Beilei Li, Xiao Li, Hui Tan, Dengfeng Cheng, Hongcheng Shi
It has been reported that the positive detection rate of Fluorine-18-fluorodeoxyglucose ((18)F-FDG) metabolism in positron emission tomography (PET) imaging, for the diagnosis of hepatocellular carcinoma (HCC) is only about 50%. In particular, (18)F-FDG PET imaging is prone to false negative findings in HCC. Transforming growth factor-beta1 (TGF-β1) shows over expression rates in early HCC liver tissue growth and promotes tumor invasion and metastases. Our aim was to use In this study, we used the feasibility of iodine-125 ((125)I)-labeled TGF-β1 antibody as a nuclear medicine imaging target in HCC...
March 20, 2017: Hellenic Journal of Nuclear Medicine
https://www.readbyqxmd.com/read/28315584/high-density-polystyrene-grafted-silver-nanoparticles-and-their-use-in-the-preparation-of-nanocomposites-with-antibacterial-properties
#11
Piotr Krystosiak, Waldemar Tomaszewski, Elżbieta Megiel
The achievement of uniform nanoparticles distribution in polymer matrix is still a major challenge in the design and fabrication of polymer nanocomposites with desired properties. In this paper we propose a novel approach for the preparation of homogeneous polystyrene/silver nanocomposites utilizing Nitroxide Mediated Radical Polymerization (NMRP). In the first step of the developed procedure, the polystyrene grafted silver nanoparticles (Ag@PS) with well-defined core-shell structure and exceptionally high grafting density (from 2 chains/nm(2) to 5...
March 9, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28314920/-new-antibiotics-standstill-or-progress
#12
J Rademacher, T Welte
The development of resistance to antibiotics has been ignored for a long time. But nowadays, increasing resistance is an important topic. For a decade no new antibiotics had been developed and it is not possible to quickly close this gap of new resistance and no new drugs. This work presents six new antibiotics (ceftaroline, ceftobiprole, solithromycin, tedizolid, ceftolozane/tazobactam, ceftazidime/avibactam). In part, only expert opinions are given due to lack of study results.The two 5th generation cephalosporins ceftaroline and ceftobiprole have beside their equivalent efficacy to ceftriaxone (ceftaroline) and cefipim (ceftobiprole) high activity against MRSA...
March 17, 2017: Medizinische Klinik, Intensivmedizin und Notfallmedizin
https://www.readbyqxmd.com/read/28314101/a-dielectrophoresis-system-for-testing-antimicrobial-susceptibility-of-gram-negative-bacteria-to-beta-lactam-antibiotics
#13
I-Hsiu Su, Wen-Chien Ko, Chung-Hsin Shih, Fang-Hao Yeh, Yung-Nien Sun, Jung-Chih Chen, Po-Lin Chen, Hsien-Chang Chang
Gram-negative bacteria (GNBs) are common pathogens causing severe sepsis. Rapid evaluation of drug susceptibility would guide effective antibiotic treatment, and promote life-saving. A total of 78 clinical isolates of 13 gram-negative species collected between April 2013 and November 2013 from two medical centers in Tainan were tested. Bacterial morphology changes in different concentrations of antibiotics were observed under the electric field of a quadruple electrode array using light microscopy. The minimal inhibitory concentrations (MICs) of four antimicrobial agents, namely cefazolin, ceftazidime, cefepime, and doripenem, were determined by the dielectrophoretic antimicrobial susceptibility testing (dAST) and by the conventional broth dilution testing (BDT)...
March 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28314049/biological-evaluation-of-3-hydroxybenzyl-alcohol-an-extrolite-produced-by-aspergillus-nidulans-strain-kzr-132
#14
C Ganesh Kumar, Poornima Mongolla, Sujitha Pombala, Shiva Bandi, K Suresh Babu, Kallaganti Venkata Siva Ramakrishna
AIMS: The aim of the study was to purify and characterize a bioactive compound from Aspergillus nidulans strain KZR-132 and its biological evaluation. METHODS AND RESULTS: A bioactive extolite was purified from Aspergillus nidulans strain KZR-132, and its chemical structure was elucidated as 3-hydroxylbenzyl alcohol (3-HBA) based on (1) H and (13) C NMR, FT-IR and mass spectroscopic analysis. The antimicrobial efficacy of 3-HBA was established against Gram-positive, Gram-negative bacteria and different Candida strains...
March 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28306563/antimicrobial-resistant-gram-negative-infections-in-neonates-burden-of-disease-and-challenges-in-treatment
#15
Laura Folgori, Julia Bielicki, Paul T Heath, Mike Sharland
PURPOSE OF REVIEW: This review summarizes the main challenges of antimicrobial resistance (AMR) in the neonatal population with a special focus on multidrug-resistant (MDR) Gram-negative pathogens. RECENT FINDINGS: MDR-Gram-negative bacteria are a great concern in the neonatal population, with a worldwide rise in the reported incidence and with very limited therapeutic options. Extended-spectrum β-lactamase and carbapenem-resistant Enterobacteriaceae (CRE) have been reported as responsible for neonatal ICU outbreaks...
March 16, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28306206/phytochemical-profile-and-biological-activities-of-helianthemum-canum-from-turkey
#16
Ayşe Baldemir, Neslihan Gökşen, Nilay Ildız, Gökçe Şeker Karatoprak, Müberra Koşar
In the current study, antioxidant, antibacterial activities, and the phenolic compositions of extracts from Helianthemum canum L. Baumg (Apiaceae) aerial parts were investigated for the first time. The H. canum was extracted with 70% methanol (HCMeOH) and water (HCW). Both extracts were determined by total phenolic contents (3 mg/mL), flavonoids (1.5 mg/mL), flavonols (1.5 mg/mL), qualitative-quantitative compositions, iron (II) chelation activities (0.1-5 mg/mL), free radical scavenging activities (DPPH(●) : 0...
March 17, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28304328/bactericidal-effect-of-pterostilbene-alone-and-in-combination-with-gentamicin-against-human-pathogenic-bacteria
#17
Wee Xian Lee, Dayang Fredalina Basri, Ahmad Rohi Ghazali
The antibacterial activity of pterostilbene in combination with gentamicin against six strains of Gram-positive and Gram-negative bacteria were investigated. The minimum inhibitory concentration and minimum bactericidal concentration of pterostilbene were determined using microdilution technique whereas the synergistic antibacterial activities of pterostilbene in combination with gentamicin were assessed using checkerboard assay and time-kill kinetic study. Results of the present study showed that the combination effects of pterostilbene with gentamicin were synergistic (FIC index < 0...
March 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28303897/synergistic-efficacy-of-bisbenzimidazole-and-carbonyl-cyanide-3-chlorophenylhydrazone-combination-against-mdr-bacterial-strains
#18
Devapriya Sinha, Stuti Pandey, Raja Singh, Vinod Tiwari, Kirti Sad, Vibha Tandon
Activation of efflux systems and the formation of biofilm are majorly adapted by microbes to resist antimicrobial agents. PPEF (bisbenzimidazole) targeting topoisomerase IA is observed to be an effective bactericidal agent against both Gram-positive and Gram-negative bacterial strains and thus can be developed as potent broad-spectrum antibiotic against MDR strains. PPEF treatment did not cause target specific mutation instead it leads to up-regulation of efflux gene in E. coli K12 as a mechanism of resistance...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28303654/live-cell-imaging-of-bacterial-cells-pyrenoylpyrrole-based-fluorescence-labelling
#19
Arun Divakar Mathiyazhagan, Sivakumar Shanmugam
A novel substituted pyrenoylpyrroles was synthesized by the reaction of pyrenoyl chalcone, TosMIC and methyl iodide under mild condition. All the synthesized compounds were screened for their bioactivity and the MIC was determined, among which few compounds showed moderate antibacterial activity towards gram-positive as well as negative bacteria. Further, cytotoxicity assay ascertained that these compounds were non-toxic to mammalian cells as well. The pyrene chromophore in the synthesized compounds (3a-e) and (5a-e) is responsible for the good photophysical properties which have an absorbance at λ 340 nm and emission at λ 410 nm...
March 17, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28303476/a-review-of-clinical-cases-of-infection-with-photorhabdus-asymbiotica
#20
John G Gerrard, Robert P Stevens
The three recognised Photorhabdus species are bioluminescent Gram-negative bacilli of the family Enterobacteriaceae. They are all pathogenic to insects and form a symbiotic relationship with nematodes of the genus Heterorhabditis. P. luminescens and P. temperata are both harmless to humans whilst P. asymbiotica, on the other hand, is a human pathogen that is a symbiont of the newly described nematode vector, Heterorhabditis gerrardi. In this chapter, we review the epidemiological and clinical features of eighteen human cases of P...
March 17, 2017: Current Topics in Microbiology and Immunology
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