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https://www.readbyqxmd.com/read/28445131/therapeutic-benefit-of-salmonella-attributed-to-lps-and-tnf-%C3%AE-is-exhaustible-and-dictated-by-tumor-susceptibility
#1
Dino Kocijancic, Sara Leschner, Sebastian Felgner, Ronja-Melinda Komoll, Michael Frahm, Vinay Pawar, Siegfried Weiss
The potential of bacteria-mediated tumor therapy (BMTT) is highlighted by more than a century of investigation. Attenuated Salmonella has prevailed as promising therapeutic agents. For BMTT - categorized as an immune therapy - the exact contribution of particular immune reactions to the therapeutic effect remains ambiguous. In addition, one could argue for or against the requirement of bacterial viability and tumor targeting. Herein we evaluate the isolated therapeutic efficacy of purified LPS and TNF-α, which together account for a dominant immunogenic pathway of gram negative bacteria like Salmonella...
April 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445079/multiple-antimicrobial-resistance-and-novel-point-mutation-in-fluoroquinolone-resistant-escherichia-coli-isolates-from-mangalore-india
#2
Santhosh Kogaluru Shivakumaraswamy, Deekshit Vijaya Kumar, Venugopal Moleyuru Nagarajappa, Iddya Karunasagar, Indrani Karunasagar
Fluoroquinolone resistance in bacteria is usually associated with mutations in the topoisomerase regions. We report a novel point mutation in fluoroquinolone-resistant Escherichia coli strains. E. coli isolated from the environment in and around Mangalore, India, were examined for their antimicrobial resistance profile to 12 antibiotics and for the antibiotic resistance genes by polymerase chain reaction. Of the 67 E. coli isolated, 24 (35.8%) were sensitive to all antibiotics and 43 (64.2%) showed resistance to at least one of the 12 antibiotics used in the study...
April 26, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28445009/evaluation-of-six-phenotypic-methods-for-the-detection-of-carbapenemases-in-gram-negative-bacteria-with-characterized-resistance-mechanisms
#3
Kunling Sun, Xiuyu Xu, Jinrong Yan, Liping Zhang
BACKGROUND: We compared the performance of the modified Hodge test (MHT), Triton Hodge test (THT), Carba NP test (CNPt), simplified Carba NP test (CNPt-direct), blue-Carba NP test (BCT), and carbapenem inactivation method (CIM) for rapid and accurate carbapenemase detection. METHODS: The methods were evaluated by using 256 gram-negative isolates, including 197 Enterobacteriaceae (79 Enterobacter spp., 74 Klebsiella spp., 33 Escherichia coli, 10 Citrobacter spp., and 1 Serratia marcescens), 51 Acinetobacter baumannii, and 8 Pseudomonas aeruginosa strains...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444620/environmental-pollution-with-antimicrobial-agents-from-bulk-drug-manufacturing-industries-in-hyderabad-south-india-is-associated-with-dissemination-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-pathogens
#4
Christoph Lübbert, Christian Baars, Anil Dayakar, Norman Lippmann, Arne C Rodloff, Martina Kinzig, Fritz Sörgel
PURPOSE: High antibiotic and antifungal concentrations in wastewater from anti-infective drug production may exert selection pressure for multidrug-resistant (MDR) pathogens. We investigated the environmental presence of active pharmaceutical ingredients and their association with MDR Gram-negative bacteria in Hyderabad, South India, a major production area for the global bulk drug market. METHODS: From Nov 19 to 28, 2016, water samples were collected from the direct environment of bulk drug manufacturing facilities, the vicinity of two sewage treatment plants, the Musi River, and habitats in Hyderabad and nearby villages...
April 26, 2017: Infection
https://www.readbyqxmd.com/read/28444602/bioactive-guided-fractionation-of-diols-from-streptomyces-sp-msl
#5
Raja Hima Bindhu Madasu, Vijayalakshmi Muvva, Rajesh Kumar Munaganti, Kumar Reddy Dorigondla, Venkateswarlu Yenamandra
An actinomycete strain with a great potential to produce bioactive compounds isolated from a laterite soil was identified as Streptomyces sp. MSL based on 16S rDNA sequence analysis. Secondary metabolites produced by the strain in optimized nutrient broth were extracted and analyzed by chromatographic and spectroscopic techniques. Among the different fractions, four diols, viz., (1) (2R,3R)-2,3-Butanediol, (2) (2R,3S)-2,3-Butanediol, (3) 2,3-dimethyl-2,3-butanediol (Pinacol), and (4) (3R)-1,3-Butanediol exhibited good antimicrobial activity...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444379/killing-the-killer-predation-between-protists-and-predatory-bacteria
#6
Julia Johnke, Jens Boenigk, Hauke Harms, Antonis Chatzinotas
Predation by microbes is one of the main drivers of bacterial mortality in the environment. In most ecosystems multiple micro-predators compete at least partially for the same bacterial resource. Predatory interactions between these micro-predators might lead to shifts within microbial communities. Integrating these interactions is therefore crucial for the understanding of ecosystem functioning. In this study we investigated the predation between two groups of micro-predators, i.e. phagotrophic protists and Bdellovibrio and like organisms (BALOs)...
April 21, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28444091/risk-factors-for-mortality-in-ventilator-associated-tracheobronchitis-a-case-control-study
#7
Leonilda Giani Pontes, Fernando Gatti de Menezes, Priscila Gonçalves, Alexandra do Rosário Toniolo, Claudia Vallone Silva, Julia Yaeko Kawagoe, Camila Marques Dos Santos, Helena Maria Fernandes Castagna, Marinês Dalla Valle Martino, Luci Corrêa
Objective: To describe the microbiological characteristics and to assess the risk factors for mortality of ventilator-associated tracheobronchitis in a case-control study of intensive care patients. Methods: This case-control study was conducted over a 6-year period in a 40-bed medical-surgical intensive care unit in a tertiary care, private hospital in São Paulo, Brazil. Case patients were identified using the Nosocomial Infection Control Committee database. For the analysis of risk factors, matched control subjects were selected from the same institution at a 1:8...
2017: Einstein
https://www.readbyqxmd.com/read/28443813/vacuolar-h-atpase-subunit-vma1p-functions-as-the-molecular-ligand-in-the-vacuole-targeting-fungicidal-activity-of-polymyxin-b
#8
Maki Iida, Keiichi Yamada, Yoshiya Nango, Yoshihiro Yamaguchi, Akira Ogita, Ken-Ichi Fujita, Toshio Tanaka
Polymyxin B (PMB) is a cationic cyclic peptide that can selectively inhibit the growth of Gram-negative bacteria by disrupting the outer membrane permeability barrier through binding to lipopolysaccharide (LPS). Here, a fluorescent PMB derivative (PMB-Ds) was applied to visually confirm the vacuole as a direct lethal target of PMB against fungal cells, which lack LPS. PMB-Ds could be visualized in the normal rounded vacuolar membrane of Saccharomyces cerevisiae cells, suggesting the presence of a molecular ligand assisting the vacuole-targeting mobilization of the peptide in the organism...
April 2017: Microbiology
https://www.readbyqxmd.com/read/28443385/pseudomonas-aeruginosa-prevalence-antibiotic-resistance-and-antimicrobial-use-in-chinese-burn-wards-from-2007-to-2014
#9
Yi Dou, Jingning Huan, Feng Guo, Zengding Zhou, Yan Shi
Objective To assess the application of antibacterial agents, alongside pathogen prevalence and Pseudomonas aeruginosa drug resistance, with the aim of understanding the impact of inappropriate antibacterial use. Methods This retrospective study assessed bacteria from wounds, catheters, blood, faeces, urine and sputum of hospitalized patients in burn wards between 2007 and 2014. The intensity of use of antibacterial agents and resistance of P. aeruginosa to common anti-Gram-negative antibiotics were measured...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28443279/analogs-of-the-frog-skin-antimicrobial-peptide-temporin-1tb-exhibit-a-wider-spectrum-of-activity-and-a-stronger-antibiofilm-potential-as-compared-to-the-parental-peptide
#10
Lucia Grassi, Giuseppantonio Maisetta, Giuseppe Maccari, Semih Esin, Giovanna Batoni
The frog skin-derived peptide Temporin 1Tb (TB) has gained increasing attention as novel antimicrobial agent for the treatment of antibiotic-resistant and/or biofilm-mediated infections. Nevertheless, such a peptide possesses a preferential spectrum of action against Gram-positive bacteria. In order to improve the therapeutic potential of TB, the present study evaluated the antibacterial and antibiofilm activities of two TB analogs against medically relevant bacterial species. Of the two analogs, TB_KKG6A has been previously described in the literature, while TB_L1FK is a new analog designed by us through statistical-based computational strategies...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28443084/gene-expression-profiling-of-transcription-factors-of-helicobacter-pylori-under-different-environmental-conditions
#11
Miguel A De la Cruz, Miguel A Ares, Kristine von Bargen, Leonardo G Panunzi, Jessica Martínez-Cruz, Hilda A Valdez-Salazar, César Jiménez-Galicia, Javier Torres
Helicobacter pylori is a Gram-negative bacterium that colonizes the human gastric mucosa and causes peptic ulcers and gastric carcinoma. H. pylori strain 26695 has a small genome (1.67 Mb), which codes for few known transcriptional regulators that control bacterial metabolism and virulence. We analyzed by qRT-PCR the expression of 16 transcriptional regulators in H. pylori 26695, including the three sigma factors under different environmental conditions. When bacteria were exposed to acidic pH, urea, nickel, or iron, the sigma factors were differentially expressed with a particularly strong induction of fliA...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28443080/genome-sequencing-reveals-the-complex-polysaccharide-degrading-ability-of-novel-deep-sea-bacterium-flammeovirga-pacifica-wpaga1
#12
Boliang Gao, Min Jin, Li Li, Wu Qu, Runying Zeng
Flammeovirga pacifica strain WPAGA1 is a Gram-negative, polysaccharide-degrading bacterium isolated from the marine sediment of the West Pacific Ocean. This strain is a cosmopolitan marine bacterium that uses complex polysaccharides as exclusive source of carbon and energy and plays a key role in the marine carbon cycle. Genome sequence analysis of strain WPAGA1 revealed that the assembled fine genome contains 6,610,326 bp with 32.89% G+C content, 5036 open reading frames (ORFs) and abundant genomic elements...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28443074/zinc-dependent-transcriptional-regulation-in-paracoccus-denitrificans
#13
Durga P Neupane, Belkis Jacquez, Anitha Sundararajan, Thiruvarangan Ramaraj, Faye D Schilkey, Erik T Yukl
Zinc homeostasis is critical for bacterial survival and is mediated largely at the transcriptional level by the regulation of zinc uptake and efflux genes. Here we use RNA-seq to assess transcriptional changes as a result of zinc limitation in the denitrifying bacterium Paracoccus denitrificans. The results identify the differential expression of 147 genes, most of which were upregulated in zinc-depleted medium. Included in this set of genes are a large number of transition metal transporters, several transcription factors, and hypothetical proteins...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28442767/direct-rapid-antimicrobial-susceptibility-test-from-positive-blood-cultures-based-on-microscopic-imaging-analysis
#14
Jungil Choi, Hyun Yong Jeong, Gi Yoon Lee, Sangkwon Han, Shinhun Han, Bonghwan Jin, Taegeun Lim, Shin Kim, Dong Young Kim, Hee Chan Kim, Eui-Chong Kim, Sang Hoon Song, Taek Soo Kim, Sunghoon Kwon
For the timely treatment of patients with infections in bloodstream and cerebrospinal fluid, a rapid antimicrobial susceptibility test (AST) is urgently needed. Here, we describe a direct and rapid antimicrobial susceptibility testing (dRAST) system, which can determine the antimicrobial susceptibility of bacteria from a positive blood culture bottle (PBCB) in six hours. The positive blood culture sample is directly mixed with agarose and inoculated into a micropatterned plastic microchip with lyophilized antibiotic agents...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28442421/distinct-roles-for-dietary-lipids-and-porphyromonas-gingivalis-infection-on-atherosclerosis-progression-and-the-gut-microbiota
#15
Carolyn D Kramer, Alexandra M Simas, Xianbao He, Robin R Ingalls, Ellen O Weinberg, Caroline Attardo Genco
Mounting evidence in humans supports an etiological role for the microbiota in inflammatory atherosclerosis. Atherosclerosis is a progressive disease characterized by accumulation of inflammatory cells and lipids in vascular tissue. While retention of lipoprotein into the sub-endothelial vascular layer is believed to be the initiating stimulus leading to the development of atherosclerosis, activation of multiple pathways related to vascular inflammation and endothelial dysfunction sustain the process by stimulating recruitment of leukocytes and immune cells into the sub-endothelial layer...
April 22, 2017: Anaerobe
https://www.readbyqxmd.com/read/28442417/cloning-expression-analysis-and-antibacterial-propertiesof-three-serum-amyloid-a-in-common-carp-cyprinus-carpio
#16
Juhua Yu, Yongkai Tang, Jianlin Li, Hongxia Li, Fan Yu, Wenjuan Yu, Feng He, Chunjie Fu, Shuntao Mao
Three serum amyloid A (SAA) genes were identified from the common carp (Cyprinus carpio) by PCR and RT-PCR. Considering both direction and sequence similarity with mammal's orthologs, they were named CcSAA3a, CcSAA3b and CcSAA1. CcSAA3b and CcSAA1 are adjacent on contig LHQP01017858, suggesting that the prototype of or the simplest SAA multigene family have occurred in common carp. A phylogenetic analysis of the SAAs indicated that the fish SAAs were closer to those of invertebrates and Ornithorhynchus anatinus, a primitive mammal, than to mammalian SAAs...
April 23, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28442305/transfer-of-kpc-2-carbapenemase-from-klebsiella-pneumoniae-to-enterobacter-cloacae-in-a-patient-receiving-meropenem-therapy
#17
Evelin Rodrigues Martins, Cássia Fernanda Estofolete, Andressa Batista Zequini, Louise Cerdeira, Doroti de Oliveira Garcia, Maria Fernanda Campagnari Bueno, Gabriela Rodrigues Francisco, Leonardo Neves de Andrade, Ana Lúcia da Costa Darini, Fernanda Modesto Tolentino, Tiago Casella, Nilton Lincopan, Mara Corrêa Lelles Nogueira
The horizontal transfer of a plasmid bearing the blaKPC-2 gene from K. pneumoniae to E. cloacae infecting the respiratory tract of a patient during meropenem therapy was elucidated. This finding is particularly worrisome, since these drugs are of last resort for multidrug-resistant Gram-negative pathogens.
April 12, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28442012/-preoperatiove-airway-bacterial-colonization-the-missing-link-between-non-small-cell-lung-cancer-following-lobectomy-and-postoperative-pneumonia
#18
Ke Gao, Yutian Lai, Jian Huang, Yifan Wang, Xiaowei Wang, Guowei Che
BACKGROUND: Surgical procedure is the main method of treating lung cancer. Meanwhile, postoperative pneumonia (POP) is the major cause of perioperative mortality in lung cancer surgery. The preoperative pathogenic airway bacterial colonization is an independent risk factor causing postoperative pulmonary complications (PPC). This cross-sectional study aimed to explore the relationship between preoperative pathogenic airway bacterial colonization and POP in lung cancer and to identify the high-risk factors of preoperative pathogenic airway bacterial colonization...
April 20, 2017: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/28441623/identification-of-a-macrobrachium-nipponense-c-type-lectin-with-a-close-evolutionary-relationship-to-vertebrate-lectins
#19
Xin Huang, Tingting Li, Min Jin, Shaowu Yin, Wen Wang, Qian Ren
C-type lectins (CTLs) are involved in the innate immune defense of vertebrates and invertebrates against invading pathogens. This study cloned and characterized a novel C-type lectin (MnCTL) of the oriental river prawn, Macrobrachium nipponense. The cloned MnCTL cDNA encompasses an open reading frame of 774 nucleotides and encodes polypeptides of 257 residues. The deduced MnCTL protein contains a single carbohydrate recognition domain (CRD) with an EPN (Glu-Pro-Asn) motif in calcium-binding site 2. Phylogenetic analysis indicated that MnCTL has a closer evolutionary relationship with vertebrate lectins than with invertebrate lectins...
April 22, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28441606/growth-inhibition-and-antibiofilm-potential-of-ag-nanoparticles-coated-with-lectin-an-arthropod-immune-molecule
#20
Sangily Jayanthi, Sathappan Shanthi, Baskaralingam Vaseeharan, Narayanan Gopi, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
Lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. Here, lectin was purified from the haemolymph of blue swimmer crab Portunus pelagicus, using mannose-coupled sepharose CL-4B affinity column chromatography. In SDS-PAGE analysis, lectin showed a molecular mass of approximately 155kDa. The synthesis of lectin-coated silver nanoparticles (lectin-coated AgNPs) was confirmed by UV-Vis spectroscopy, XRD, FTIR, SEM, TEM, SAED, and EDX analysis...
April 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
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