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https://www.readbyqxmd.com/read/29778961/uva-induced-antimicrobial-activity-of-zno-ag-nanocomposite-covered-surfaces
#1
Meeri Visnapuu, Merilin Rosenberg, Egle Truska, Ergo Nõmmiste, Andris Šutka, Anne Kahru, Mihkel Rähn, Heiki Vija, Kaja Orupõld, Vambola Kisand, Angela Ivask
Application of efficient antimicrobial surfaces has been estimated to decrease both, the healthcare-associated infections and the spread of antibiotic-resistant bacteria. In this paper, we prepared ZnO and ZnO/Ag nanoparticle covered surfaces and evaluated their antimicrobial efficacy towards a Gram-negative bacterial model (Escherichia coli), a Gram-positive bacterial model (Staphylococcus aureus) and a fungal model (Candida albicans) in the dark and under UVA illumination. The surfaces were prepared by spin coating aliquots of ZnO and ZnO/Ag nanoparticle suspensions onto glass substrates...
May 4, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29778819/detection-of-tem-and-ctx-m-genes-from-ciprofloxacin-resistant-proteus-mirabilis-and-escherichia-coli-isolated-on-urinary-tract-infections-utis
#2
Govindan Rajivgandhi, Muthuchamy Maruthupandy, Natesan Manoharan
The multidrug resistant Gram negative bacteria (MDRGNB) is an emerging burden and now represents a daily challenge for the management of antimicrobial therapy in healthcare settings. The present study was aimed to detect the prevalence of TEM and CTX-M type genes from GNB on urinary tract infection (UTIs). The ciprofloxacin resistant uropathogens were detected by HEXA UTI 5 disc diffusion method. The phenotypic detection of uropathogens producing extended spectrum beta lactamases (ESBLs) was confirmed by double disc combination (DDCT) and phenotype confirmation methods (PCT)...
May 17, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29778482/randomized-noninferiority-field-trial-evaluating-cephapirin-sodium-for-treatment-of-nonsevere-clinical-mastitis
#3
T Tomazi, T A F Lopes, V Masson, J M Swinkels, M V Santos
The general objective of this study was to evaluate whether cephapirin sodium is noninferior compared with a positive control broad-spectrum product formulated with a combination of antimicrobials for intramammary treatment of nonsevere clinical mastitis. In addition, we compared the efficacy of treatments on the cure risks of pathogen groups (gram-positive, gram-negative, and cultures with no growth) based on culture results. A total of 346 cows distributed in 31 commercial dairy herds were selected to participate in the study, although only 236 met the criteria for evaluation of microbiological cure...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29778319/anti-infective-biomaterials-with-surface-decorated-tachyplesin-i
#4
Qiong Xue, Xiao-Bin Liu, Yeh-Hsing Lao, Lin-Ping Wu, Dong Wang, Zhen-Qiang Zuo, Jun-Yu Chen, Jing Hou, Yuan-Yuan Bei, Xue-Fei Wu, Kam W Leong, Hua Xiang, Jing Han
Implants decorated with antimicrobial peptides (AMPs) can prevent infection and reduce the risk of creating antibiotic resistance. Yet the restricted mobility of surficial AMP often compromises its activity. Here, we report a simple but effective strategy to allow a more flexible display of AMP on the biomaterial surface and demonstrate its efficacy for wound healing. The AMP, tachyplesin I (Tac), is tagged with the polyhydroxyalkanoate-granule-associated protein (PhaP) and immobilized on haloarchaea-produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) via hydrophobic interaction...
May 9, 2018: Biomaterials
https://www.readbyqxmd.com/read/29778213/nitric-oxide-an-old-molecule-with-noble-functions-in-pseudomonas-aeruginosa-biology
#5
Masanori Toyofuku, Sang-Sun Yoon
Pseudomonas aeruginosa, a Gram-negative bacterium, is characterized by its versatility that enables persistent survival under adverse conditions. It can grow on diverse energy sources and readily acquire resistance to antimicrobial agents. As an opportunistic human pathogen, it also causes chronic infections inside the anaerobic mucus airways of cystic fibrosis patients. As a strict respirer, P. aeruginosa can grow by anaerobic nitrate ( [Formula: see text] ) respiration. Nitric oxide (NO) produced as an intermediate during anaerobic respiration exerts many important effects on the biological characteristics of P...
2018: Advances in Microbial Physiology
https://www.readbyqxmd.com/read/29778048/pathogens-constancy-harbinger-of-nosocomial-infection-cum-identification-of-resistant-genes-and-drug-designing
#6
Sana Farooq Ahmad, Ibrar Khan, Abdul Wadood, Sadiq Azam, Noor Rehman, Muhammad Waqas, Kashif Bashir, Asaf Ali Khan
Hospitals are the most prominent places for the growth and spread of bacteria which are resistant to the available antibiotics. This antibiotic resistance is due to the over and misuse of antibiotic dosages of a high-density of patient population which are in frequent interaction with inanimate items of the hospitals and the consequent risk of cross infection. Out of 1010 samples of the current study, 510 (50.49%) were culture positive of which 329 (64.5%) were Gram-positive while 181 (35.49%) Gram negative...
April 22, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29777987/phthalocyanine-sulfonamide-conjugates-synthesis-and-photodynamic-inactivation-of-gram-negative-and-gram-positive-bacteria
#7
Raquel Nunes da Silva, Ângela Cunha, Augusto C Tomé
Phthalocyanines bearing four or eight sulfonamide units were synthesized and their efficiency in the photodynamic inactivation of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria was evaluated. Conjugates with simpler sulfonamide units (N,N-diethylbenzenesulfonamide, N-isopropylbenzenesulfonamide and N-(4-methoxyphenyl)benzenesulfonamide) caused stronger inactivation than those with heterocyclic groups (N-(thiazol-2-yl)benzenesulfonamide) or long alkyl chains (N-dodecylbenzenesulfonamide) in both bacteria...
May 9, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29777807/identification-of-polysaccharides-extracted-from-pea-pod-by-products-and-evaluation-of-their-biological-and-functional-properties
#8
Lilia Belghith-Fendri, Fatma Chaari, Khawla Ben Jeddou, Fatma Kallel, Fatma Bouaziz, Claire Boisset Helbert, Lobna Abdelkefi-Mesrati, Semia Ellouz-Chaabouni, Dhouha Ghribi-Aydi
A three-variable Box-Behnken design was employed to obtain the best possible combination of extraction time, ratio (raw material/water) and extraction temperature to allow maximum extraction yield of polysaccharides from pea pod (PPP). The preferred extraction conditions were: extraction time 195 min, extraction temperature 70 °C and ratio of raw material/water 1/40. Under these conditions, the experimental yield was 16.21 ± 1.12%, which is in close agreement with the value predicted by response surface methodology model yield (16...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29777720/regulation-of-shigella-effector-kinase-ospg-through-modulation-of-its-dynamic-properties
#9
Andrey M Grishin, Kathryn R Barber, Ruo-Xu Gu, D Peter Tieleman, Gary S Shaw, Miroslaw Cygler
Gram-negative pathogens secrete effector proteins into human cells to modulate normal cellular processes and establish a bacterial replication niche. Shigella and pathogenic E. coli possess homologous effector kinases, OspG and NleH1/2, respectively. Upon translocation, OspG but not NleH binds to ubiquitin and a subset of E2~Ub conjugates, which was shown to activate its kinase activity. Here we show that OspG, having a minimal kinase fold, acquired a novel mechanism of regulation of its activity. Binding of the E2~Ub conjugate to OspG not only stimulates its kinase activity but also increases its optimal temperature for activity to match the human body temperature and stabilizes its labile C-terminal domain...
May 16, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29777669/repair-of-thoracic-and-thoracoabdominal-mycotic-aneurysms-and-infected-aortic-grafts-using-allograft
#10
Joel S Corvera, David Blitzer, Hannah Copeland, Daniel Murphy, Philip J Hess, Saila T Pillai, John W Fehrenbacher
BACKGROUND: Mycotic aneurysm of the thoracic or thoracoabdominal aorta and infection of thoracic or thoracoabdominal aortic grafts are challenging problems with high mortality. In-situ reconstruction with cryopreserved allograft(CPA) avoids placement of prosthetic material in an infected field and avoids suppressive antibiotics or autologous tissue coverage. METHODS: Fifty consecutive patients with infection of a thoracic or thoracoabdominal aortic graft or mycotic aneurysm underwent resection and replacement with CPA from 2006 to 2016...
May 16, 2018: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29776609/threshold-level-of-riemerella-anatipestifer-crossing-blood-brain-barrier-and-expression-profiles-of-immune-related-proteins-in-blood-and-brain-tissue-from-infected-ducks
#11
Shengdou Li, Xiaowei Gong, Qiwei Chen, Fuying Zheng, Guo Ji, Yongsheng Liu
Riemerella anatipestifer (R. anatipestifer) is a Gram-negative bacterium that crosses the blood-brain barrier (BBB) and causes meningitis with neurological symptoms in infected ducks. A threshold level of bacteremia must be reached before the BBB can be breached. In this study, the bacterial burden and expression profiles of immune-related proteins in blood and brain tissue samples from R. anatipestifer-infected ducks were investigated. The data showed that R. anatipestifer could cross the BBB with low-level bacteremia of 7...
June 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29775788/complete-genome-sequence-of-achromobacter-spanius-type-strain-dsm-23806t-a-pathogen-isolated-from-human-blood
#12
Gengmi Li, Li Yang, Tao Zhang, Xiaojiao Guo, Jian Qin, Yingjiang Cao, Qianhua Yang, Shumei You, Guoliang Yuan, Xianqi Wan, Jing Luo, Zhaoxiang Li, Lei Gao, Ying Liu, Kaifeng Jiang, Jiakui Zheng
OBJECTIVES: Achromobacter spanius is a new described, non-fermenting Gram-negative and coccoid pathogen that isolated from patient blood. In order to investigate the pathogenesis of this strain in genomic level, whole genome sequencing was carried out on this strain. METHODS: In this study, complete genome of the type strain of this species DSM 23806T was sequenced using single-molecule real-time (SMRT) DNA sequencing. RESULTS: Complete genome of DSM 23806T consists of one circular DNA chromosome of 6,425,783bp with a GC content of 64...
May 15, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29775786/is-mic-increase-of-meropenem-against-klebsiella-pneumoniae-carbapenemase-kpc-producing-klebsiella-pneumoniae-correlated-with-the-increase-of-resistance-rates-against-some-other-antibiotics-with-gram-negative-activity
#13
Piergiorgio Cojutti, Assunta Sartor, Matteo Bassetti, Claudio Scarparo, Federico Pea
OBJECTIVE: To assess MIC distribution for meropenem and the other antibiotics with Gram-negative activity against KPC-Kp clinical isolates collected at our hospital in 2013-2016. METHODS: Susceptibility of KPC-Kp strains was tested by broth microdilution method, using customized 96-well plates, and was interpreted according to the EUCAST recommendations. RESULTS: Among 169 consecutive KPC-Kp clinical isolates 26.6% were susceptible to meropenem...
May 15, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29775705/effects-of-different-chemical-modifications-on-the-antibacterial-activities-of-polysaccharides-sequentially-extracted-from-peony-seed-dreg
#14
Xiao-Li Li, Kiran Thakur, Yuan-Yuan Zhang, Xiao-Fang Tu, Ying-Shuo Zhang, Dan-Ye Zhu, Jian-Guo Zhang, Zhao-Jun Wei
The peony seed dreg polysaccharide (PSDPs) fractions and their modified derivatives were examined for their antibacterial potential. PSDPs along with their derivatives exhibited appreciable antibacterial activity against both Gram-positive (B. subtilis and S. aureus) as well as Gram-negative (E. coli and S. typhimurium) bacteria. Among the four polysaccharide fractions, HBSS possessed the greatest antibacterial activity with an inhibition zone of 13.6 mm against S. typhimurium, minimal inhibitory concentration of 0...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775685/phenotypic-and-genetic-resistance-traits-in-pseudomonas-aeruginosa-strains-infecting-cystic-fibrosis-patients-a-french-cohort-study
#15
Nathalie Courtois, Yvan Caspar, Max Maurin
Pseudomonas aeruginosa is responsible for chronic respiratory tract colonisation and acute exacerbations in cystic fibrosis (CF) patients. This Gram-negative bacterium often develops multidrug resistance, which represents a therapeutic challenge. Our objective was to characterize the phenotypic and genetic β-lactam resistance traits of P. aeruginosa strains isolated from CF patients followed at Grenoble Alpes university hospital. The susceptibility to β-lactam compounds of 123 P. aeruginosa strains collected from the lower respiratory tract of 45 CF patients, between 2010 and 2014, was evaluated...
May 15, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29775554/outcomes-and-predictors-of-early-infection-after-heart-transplantation
#16
Kendall C Shultes, Jerrica E Shuster, Scott Micek, Justin M Vader, Keki Balsara, Akinobu Itoh, Bethany R Tellor
BACKGROUND: Limited data exist on the incidence and outcome of early infection after orthotopic heart transplantation (OHT). The purpose of this study was to describe characteristics and outcomes of OHT recipients with an early infection and to identify predictors of such infections. METHODS: This retrospective, single-center study included patients greater than 18 years of age who underwent OHT from February 2009 to May 2014 and had an infection within 30 days of transplantation...
May 18, 2018: Surgical Infections
https://www.readbyqxmd.com/read/29775486/peroxidasin-contributes-to-lung-host-defense-by-direct-binding-and-killing-of-gram-negative-bacteria
#17
Ruizheng Shi, Zehong Cao, Hong Li, Jochen Graw, Guogang Zhang, Victor J Thannickal, Guangjie Cheng
Innate immune recognition is classically mediated by the interaction of host pattern-recognition receptors and pathogen-associated molecular patterns; this triggers a series of downstream signaling events that facilitate killing and elimination of invading pathogens. In this report, we provide the first evidence that PXDN (also known as vascular peroxidase-1) directly binds to gram-negative bacteria and mediates bactericidal activity, thus, contributing to lung host defense. PXDN contains five leucine-rich repeats and four immunoglobulin domains, which allows for its interaction with lipopolysaccharide, a membrane component of gram-negative bacteria...
May 18, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29775296/incorporation-of-a-theranostic-two-tone-luminescent-silver-complex-into-biocompatible-agar-hydrogel-composite-for-the-eradication-of-eskape-pathogens-in-a-skin-and-soft-tissue-infection-model
#18
Miguel N Pinto, Jorge Martinez-Gonzalez, Indranil Chakraborty, Pradip K Mascharak
Microbial invasion and colonization of the skin and underlying soft tissues are among the most common types of infections, becoming increasingly prevalent in hospital settings. Systemic antibiotic chemotherapies are now extremely limited due to emergence of drug-resistant Gram-positive and multidrug-resistant Gram-negative bacterial strains. Topical administration of antimicrobials provides an effective route for the treatment of skin and soft tissue infections (SSTIs). Therefore, the development of new and effective materials for the delivery of these agents is of paramount importance...
May 18, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29775177/novosphingobium-arvoryzae-sp-nov-isolated-from-a-flooded-rice-field
#19
Shih-Yi Sheu, Cheng-Wen Huang, Jhen-Ci Chen, Zih-Han Chen, Wen-Ming Chen
A bacterial strain, designated Jyi-02T , was isolated from a flooded rice field in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain Jyi-02T were aerobic, Gram-stain-negative, rod-shaped, non-motile and formed yellowish orange coloured colonies. Growth occurred at 10-40 °C (optimum, 20 °C) and pH 5.0-9.0 (optimum, pH 7.0) and in the presence of 0-1.0 % NaCl (optimum, 0 %, w/v). The major fatty acids (>10 %) of strain Jyi-02T were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C14 : 0 2-OH...
May 18, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29775176/chitinophaga-varians-sp-nov-isolated-from-forest-soil
#20
Ying-Ying Lv, Xian-Jiao Zhang, An-Zhang Li, Wei-Ling Zou, Guang-da Feng, Hong-Hui Zhu
A Gram-stain-negative, aerobic, non-motile bacterium, designated strain 10-7W-9003T , was isolated from the forest soil of Limushan National Forest Park, south-east China (19° 10' 42″ N, 109° 44' 45″ E). Strain 10-7W-9003T showed a shape change during the course of culture from long filamentous cells (5-10×0.4-0.5 µm) at 5-36 h, to rod shaped (1.0-1.5×0.5-0.7 µm) with inoculation after 2 days. It grew optimally at 28-30 °C and pH 6.5-7.5. On the basis of 16S rRNA gene sequence analysis, it belongs to the genus Chitinophaga and is most closely related to Chitinophaga eiseniae KACC 13774T and Chitinophaga qingshengii JCM 30026T , with 16S rRNA gene sequences similarities of 98...
May 18, 2018: International Journal of Systematic and Evolutionary Microbiology
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