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https://www.readbyqxmd.com/read/28817647/caprine-brucellosis-a-historically-neglected-disease-with-significant-impact-on-public-health
#1
REVIEW
Carlos A Rossetti, Angela M Arenas-Gamboa, Estefanía Maurizio
Caprine brucellosis is a chronic infectious disease caused by the gram-negative cocci-bacillus Brucella melitensis. Middle- to late-term abortion, stillbirths, and the delivery of weak offspring are the characteristic clinical signs of the disease that is associated with an extensive negative impact in a flock's productivity. B. melitensis is also the most virulent Brucella species for humans, responsible for a severely debilitating and disabling illness that results in high morbidity with intermittent fever, chills, sweats, weakness, myalgia, abortion, osteoarticular complications, endocarditis, depression, anorexia, and low mortality...
August 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28817268/natural-organic-matter-concentration-impacts-the-interaction-of-functionalized-diamond-nanoparticles-with-model-and-actual-bacterial-membranes
#2
Arielle C Mensch, Rodrigo Tapia Hernandez, Joshua E Kuether, Marco D Torelli, Z Vivian Feng, Robert J Hamers, Joel A Pedersen
Changes to nanoparticle surface charge, colloidal stability, and hydrodynamic properties induced by interaction with natural organic matter (NOM) warrant consideration in assessing the potential for these materials to adversely impact organisms in the environment. Here we show that acquisition of a coating, or "corona", of NOM alters the hydrodynamic and electrokinetic properties of diamond nanoparticles (DNPs) functionalized with the polycation poly(allylamine HCl) in a manner that depends on the NOM-to-DNP concentration ratio...
August 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28816602/synthesis-and-in-vitro-antimicrobial-and-antioxidant-activities-of-quaternary-ammonium-chitosan-modified-with-nisin
#3
Lian Min, Meng Liu, Chen Zhu, Liangling Liu, Zhiqie Rao, Lihong Fan
Nisin had been grafted onto quaternary ammonium chitosan (QCS) through an enzyme-catalyzed reactionto enhanceitslimitedantimicrobial activity. QCS was synthesizedby incorporating N-(3-chloro-2-hydroxypropyl) trimethyl ammonium chloride(CHPTAC)ontochitosan's primary aminegroup.The modificationhad been confirmed by FT-IR and (1)H NMR spectroscopy. Degree of substitution (DS) of QCS-nisin could be controlled by adjusting the reaction conditions. The synthesized compounds were screened in vitroto evaluate their antimicrobial and antioxidant activities...
August 17, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28816443/characterization-of-ciprofloxacin-permeation-pathways-across-the-porin-ompc-using-metadynamics-and-a-string-method
#4
Jigneshkumar Dahyabhai Prajapati, Carlos José Fernández Solano, Mathias Winterhalter, Ulrich Kleinekathöfer
The rapid spreading of antimicrobial resistance in Gram-negative bacteria has become a major threat for humans as well as animals. As one of the main factors involved, the permeability of the outer membrane has attracted quite some attention recently. However, the knowledge regarding the translocation mechanisms for most available antibiotics is so far rather limited. Here, a theoretical study concerning the diffusion route of ciprofloxacin across the outer membrane porin OmpC from E. coli is presented. To this end, we establish a protocol to characterize meaningful permeation pathways by combining metadynamics with the zero-temperature string method...
August 17, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28815948/flow-cytometric-analysis-of-viable-bacteria-in-urine-samples-of-febrile-patients-at-the-emergency-department
#5
Anne H Tavenier, Foppie J de Boer, Bijan Moshaver, Sjef J C M van der Leur, Coen A Stegeman, Paul H P Groeneveld
BACKGROUND: Fast and reliable diagnostics are important in febrile patients admitted to the emergency department. Current urine diagnostics are fast but moderately reliable or reliable but time consuming. Flow cytometry (FC) is a new promising technique in the diagnostics of complicated urinary tract infections by counting bacteria in urine samples. The aim of this study is to improve the FC method by counting only viable bacteria. METHODS: Urine was obtained from 135 consecutive febrile patients at the emergency department...
August 16, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28815827/outer-membrane-lipid-homeostasis-via-retrograde-phospholipid-transport-in-escherichia-coli
#6
Rahul Shrivastava, Xiang'Er Jiang, Shu-Sin Chng
Biogenesis of the outer membrane (OM) in Gram-negative bacteria, which is essential for viability, requires the coordinated transport and assembly of proteins and lipids, including lipopolysaccharides (LPS) and phospholipids (PLs), into the membrane. While pathways for LPS and OM protein assembly are well-studied, how PLs are transported to and from the OM is not clear. Mechanisms that ensure OM stability and homeostasis are also unknown. The trans-envelope Tol-Pal complex, whose physiological role has remained elusive, is important for OM stability...
August 16, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28815732/antibacterial-and-%C3%AE-lactamase-inhibitory-activity-of-monocyclic-%C3%AE-lactams
#7
REVIEW
Lena Decuyper, Marko Jukič, Izidor Sosič, Aleš Žula, Matthias D'hooghe, Stanislav Gobec
Due to the widespread emergence of resistant bacterial strains, an urgent need for the development of new antibacterial agents with novel modes of action has emerged. The discovery of naturally occurring monocyclic β-lactams in the late 1970s, mainly active against aerobic Gram-negative bacteria, has introduced a new approach in the design and development of novel antibacterial β-lactam agents. The main goal was the derivatization of the azetidin-2-one core in order to improve their antibacterial potency, broaden their spectrum of activity, and enhance their β-lactamase stability...
August 16, 2017: Medicinal Research Reviews
https://www.readbyqxmd.com/read/28815637/synergistic-antibacterial-and-antibiofilm-efficacy-of-nisin-in-combination-with-p-coumaric-acid-against-foodborne-bacteria-bacillus-cereus-and-salmonella-typhimurium
#8
Anwesa Bag, Rabi Ranjan Chattopadhyay
The aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria Bacillus cereus and Salmonella typhimurium. Their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. Nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both the studied bacteria whereas nisin/ linalool combination showed synergistic activity against B...
August 16, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28815210/lpxk-is-essential-for-growth-of-acinetobacter-baumannii-atcc-19606-relationship-to-toxic-accumulation-of-lipid-a-pathway-intermediates
#9
Jun-Rong Wei, Daryl L Richie, Mina Mostafavi, Louis E Metzger, Christopher M Rath, William S Sawyer, Kenneth T Takeoka, Charles R Dean
Acinetobacter baumannii ATCC 19606 can grow without lipid A, the major component of lipooligosaccharide. However, we previously reported that depletion of LpxH (the fourth enzyme in the lipid A biosynthetic pathway) prevented growth of this strain due to toxic accumulation of lipid A pathway intermediates. Here, we explored whether a similar phenomenon occurred with depletion of LpxK, a kinase that phosphorylates disaccharide 1-monophosphate (DSMP) at the 4' position to yield lipid IVA. An A. baumannii ATCC 19606 derivative with LpxK expression under the control of an isopropyl β-d-1-thiogalactopyranoside (IPTG)-regulated expression system failed to grow without induction, indicating that LpxK is essential for growth...
July 2017: MSphere
https://www.readbyqxmd.com/read/28815095/acinetobacter-lwoffii-peritonitis-in-a-patient-on-automated-peritoneal-dialysis-a-case-report-and-review-of-the-literature
#10
Murat Yasar Tas, Meral Merve Oguz, Mevlut Ceri
Acinetobacter lwoffii, a nonfermentative gram-negative aerobic bacillus, which presents in the normal flora of the oropharynx and skin, has recently been reported as a cause of human infection. Herein, the authors present a case report of peritonitis related to automated peritoneal dialysis caused by A. lwoffii.
2017: Case Reports in Nephrology
https://www.readbyqxmd.com/read/28815094/add-on-therapy-with-ertapenem-in-infections-with-multidrug-resistant-gram-negative-bacteria-pediatric-experience
#11
Sevgen Tanır Basaranoglu, Yasemin Ozsurekci, Kubra Aykac, Kamile Oktay Arıkan, Ayse Buyukcam, Ali Bulent Cengiz, Mehmet Ceyhan, Ates Kara
Optimal therapy for infections with carbapenem resistant GNB is not well established due to the weakness of data. Patients presenting with bloodstream infections caused by multidrug resistant Klebsiella pneumoniae were treated with a combination treatment. Optimal therapy for infections with carbapenem resistant Gram-negative bacteria is a serious problem in pediatric patients. We presented three cases who were successfully treated with addition of ertapenem to the combination treatment for bacteremia with multidrug resistant Klebsiella pneumoniae...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28815056/hemojuvelin-regulates-the-innate-immune-response-to-peritoneal-bacterial-infection-in-mice
#12
Qian Wu, Yuanyuan Shen, Yunlong Tao, Jiayu Wei, Hao Wang, Peng An, Zhuzhen Zhang, Hong Gao, Tianhua Zhou, Fudi Wang, Junxia Min
Hereditary hemochromatosis and iron imbalance are associated with susceptibility to bacterial infection; however, the underlying mechanisms are poorly understood. Here, we performed in vivo bacterial infection screening using several mouse models of hemochromatosis, including Hfe (Hfe(-/-) ), hemojuvelin (Hjv(-/-) ), and macrophage-specific ferroportin-1 (Fpn1(fl/fl) ;LysM-Cre(+) ) knockout mice. We found that Hjv(-/-) mice, but not Hfe(-/-) or Fpn1(fl/fl) ;LysM-Cre(+) mice, are highly susceptible to peritoneal infection by both Gram-negative and Gram-positive bacteria...
2017: Cell Discovery
https://www.readbyqxmd.com/read/28814302/ompa-protein-sequence-based-typing-and-virulence-associated-gene-profiles-of-pasteurella-multocida-isolates-associated-with-bovine-haemorrhagic-septicaemia-and-porcine-pneumonic-pasteurellosis-in-thailand
#13
Teerasak E-Kobon, Ratiporn Leeanan, Saengtian Pannoi, Pornchai Anuntasomboon, Pacharee Thongkamkoon, Arinthip Thamchaipenet
BACKGROUND: Pasteurella multocida is a Gram-negative bacterium that causes economically significant infections of a broad range of animal species. Pneumonic and septicaemic pasteurellosis caused by this bacterium remain important problems in pigs, cattle, and water buffaloes in Thailand. The aim of this study was to characterise the virulence-associated gene profiles and to develop an OmpA molecular typing scheme for classifying 191 bovine and porcine isolates of P. multocida collected between 1989 and 2012 in Thailand using polymerase chain reactions (PCRs), nucleotide sequencing, and sequence and structural bioinformatics analyses...
August 16, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28814300/increasing-on-target-cleavage-efficiency-for-crispr-cas9-induced-large-fragment-deletion-in-myxococcus-xanthus
#14
Ying-Jie Yang, Ye Wang, Zhi-Feng Li, Ya Gong, Peng Zhang, Wen-Chao Hu, Duo-Hong Sheng, Yue-Zhong Li
BACKGROUND: The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potential off-target DNA cleavage from incorrect guide by the 20 nt spacer. RESULTS: In this study, after resolving some critical limits, we have established an efficient CRISPR/Cas9 system for the deletion of large genome fragments related to the biosynthesis of secondary metabolites in Myxococcus xanthus cells...
August 16, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28813752/atypical-presentations-of-tularemia
#15
Karah Odegaard, Beth Boersma, James Keegan
Francisella tularensis is a gram-negative coccobacillus that causes a condition commonly referred to as tularemia. There has been a dramatic increase in tularemia cases reported in South Dakota, many of which were challenging to diagnose due to atypical clinical manifestations. We describe an interesting case of pneumonic tularemia and summarize six similar cases, several of which presented with lung nodules suggestive of malignancy. According to the literature, this is only the third outbreak of pneumonic tularemia reported in the U...
May 2017: South Dakota Medicine: the Journal of the South Dakota State Medical Association
https://www.readbyqxmd.com/read/28813680/antibiotics-disrupt-coordination-between-transcriptional-and-phenotypic-stress-responses-in-pathogenic-bacteria
#16
Paul A Jensen, Zeyu Zhu, Tim van Opijnen
Bacterial genes that change in expression upon environmental disturbance have commonly been seen as those that must also phenotypically matter. However, several studies suggest that differentially expressed genes are rarely phenotypically important. We demonstrate, for Gram-positive and Gram-negative bacteria, that these seemingly uncoordinated gene sets are involved in responses that can be linked through topological network analysis. However, the level of coordination is stress dependent. While a well-coordinated response is triggered in response to nutrient stress, antibiotics trigger an uncoordinated response in which transcriptionally and phenotypically important genes are neither linked spatially nor in their magnitude...
August 15, 2017: Cell Reports
https://www.readbyqxmd.com/read/28813510/construction-of-novel-pjrd215-derived-plasmids-using-chloramphenicol-acetyltransferase-cat-gene-as-a-selection-marker-for-acidithiobacillus-caldus
#17
Rui Wang, Chunmao Lin, Jianqiang Lin, Xin Pang, Xiangmei Liu, Chengjia Zhang, Jianqun Lin, Linxu Chen
BACKGROUND: Acidithiobacillus caldus, a Gram-negative, chemolithotrophic sulfur-oxidizing bacterium, is widely applied in bioleaching. The absence of an ideal selection marker has become a major obstacle to achieve high efficiency of the gene transfer system for A. caldus. Plasmid pJRD215, widely used in Acidithiobacillus spp., has severe drawbacks in molecular manipulations and potential biosafety issues due to its mobility. Therefore, finding a new selection marker and constructing new plasmids have become an urgent and fundamental work for A...
2017: PloS One
https://www.readbyqxmd.com/read/28813014/antibacterial-properties-of-nonwoven-wound-dressings-coated-with-manuka-honey-or-methylglyoxal
#18
Sophie E L Bulman, Giuseppe Tronci, Parikshit Goswami, Chris Carr, Stephen J Russell
Manuka honey (MH) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. MH provides unique antibacterial activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (MGO). Aiming to investigate an antibiotic-free antimicrobial strategy, we studied the antibacterial activity of both MH and MGO (at equivalent MGO concentrations) when applied as a physical coating to a nonwoven fabric wound dressing. When physically coated on to a cellulosic hydroentangled nonwoven fabric, it was found that concentrations of 0...
August 16, 2017: Materials
https://www.readbyqxmd.com/read/28812884/controlling-bdellovibrio-bacteriovorus-gene-expression-and-predation-using-synthetic-riboswitches
#19
Mohammed Dwidar, Yohei Yokobayashi
Bdellovibrio bacteriovorus is a predatory bacterium that feeds on Gram-negative bacteria including a wide range of pathogens and thus has potential applications as a biocontrol agent. Owing to its unique life cycle, however, there are limited tools that enable genetic manipulation of B. bacteriovorus. This work describes our first steps towards engineering the predatory bacterium for practical applications by developing basic genetic parts to control gene expression. Specifically, we evaluated four robust promoters that are active during the attack phase of B...
August 16, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28812532/spontaneous-experimentally-induced-and-transmissible-aa-amyloidosis-in-japanese-quail-coturnix-japonica
#20
Yumi Nakayama, Junichi Kamiie, Gen Watanabe, Kazuhiko Suzuki, Tomoaki Murakami
The authors describe a spontaneous case of amyloid A (AA) amyloidosis in an adult female Japanese quail ( Coturnix japonica). The bird developed AA amyloidosis secondary to chronic peritonitis caused by a Gram-negative bacillus infection. Mild amyloid deposition was also identified in the intestinal tract of apparently healthy adult individuals, suggesting that quail may develop intestinal amyloidosis with age. Based on these observations, it was hypothesized that quail can develop AA amyloidosis following inflammatory stimulation with lipopolysaccharide (LPS)...
January 1, 2017: Veterinary Pathology
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