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https://www.readbyqxmd.com/read/28550976/cultivation-and-characterization-of-pterygium-as-an-ex-vivo-study-model-for-disease-and-therapy
#1
Natasha Josifovska, Dóra Júlia Szabó, Richárd Nagymihály, Zoltán Veréb, Andrea Facskó, Ketil Eriksen, Morten C Moe, Goran Petrovski
PURPOSE: Development of ex vivo model to study pathogenesis, inflammation and treatment modalities for pterygium. METHODS: Pterygium obtained from surgery was cultivated (3 months). Gravitational attachment method using viscoelastic facilitated adherence of graft and outgrowing cells. Medium contained serum as the only growth supplement with no use of scaffolds. Surface profiling of the multi-layered cells for hematopoietic- and mesenchymal stem cell markers was performed...
May 24, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28545230/nanog-plays-a-hierarchical-role-in-the-transcription-network-regulating-the-pluripotency-and-plasticity-of-adipose-tissue-derived-stem-cells
#2
Maria Pitrone, Giuseppe Pizzolanti, Laura Tomasello, Antonina Coppola, Lorenzo Morini, Gianni Pantuso, Romina Ficarella, Valentina Guarnotta, Sebastio Perrini, Francesco Giorgino, Carla Giordano
The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self-renewal potential and differentiation capacity. Our aim was to study the different expression of the embryonic stem cell markers NANOG (homeobox protein NANOG), SOX2 (SRY (sex determining region Y)-box 2) and OCT4 (octamer-binding transcription factor 4) and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT...
May 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28541092/amniotic-mesenchymal-stromal-cells-exhibit-preferential-osteogenic-and-chondrogenic-differentiation-and-enhanced-matrix-production-compared-with-adipose-mesenchymal-stromal-cells
#3
Natasha Topoluk, Richard Hawkins, John Tokish, Jeremy Mercuri
BACKGROUND: Therapeutic efficacy of various mesenchymal stromal cell (MSC) types for orthopaedic applications is currently being investigated. While the concept of MSC therapy is well grounded in the basic science of healing and regeneration, little is known about individual MSC populations in terms of their propensity to promote the repair and/or regeneration of specific musculoskeletal tissues. Two promising MSC sources, adipose and amnion, have each demonstrated differentiation and extracellular matrix (ECM) production in the setting of musculoskeletal tissue regeneration...
May 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28537471/characteristics-of-labial-gland-mesenchymal-stem-cells-of-healthy-individuals-and-patients-with-sj%C3%A3-gren-syndrome-a-preliminary-study
#4
Shiqin Wang, Hong Hua, Yixiang Wang
Sjögren's Syndrome (SS) is a systemic autoimmune disease characterized by focal lymphocytic infiltration into exocrine organs such as salivary and lacrimal glands, resulting in dry mouth and eyes, and other systemic injuries. The mesenchymal stem cells (MSCs) in the salivary glands of healthy individuals and patients with SS have not been extensively studied. The aim of this study was to eluciadate the characteristics of MSCs from the labial glands of healthy controls and of those from patients with SS in order to elucidate the related pathogenesis and uncover potential avenues for novel clinical interventions...
May 24, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28531915/bone-marrow-stem-cells-anti-liver-fibrosis-potency-inhibition-of-hepatic-stellate-cells-activity-and-extracellular-matrix-deposition
#5
Ervina Julien Sitanggang, Radiana Dhewayani Antarianto, Sri Widia A Jusman, Jeanne Adiwinata Pawitan, Ahmad Aulia Jusuf
Transplantation of bone marrow derived stem cells (BMSCs) has been reported inhibits liver fibrosis. Several in vitro studies by co-culturing BMSCs and hepatic stellate cells (HSCs) indirectly or directly in 2D models showed inhibition of HSC as the key player in liver fibrosis. In this study, we investigated direct effect of BMSCs on HSCs by co-culturing BMSCs and HSCs in 3D model as it represents the liver microenvironment with intricate cell-cell and cell-matrix interactions. Primary isolated rat HSCs and BMSCs were directly co-cultured at 1:1 ratio with hanging drop method...
May 30, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28531813/immunomodulatory-function-of-whole-human-umbilical-cord-derived-mesenchymal-stem-cells
#6
Hao Zhang, Yanling Tao, Haihui Liu, Saisai Ren, Bin Zhang, Hu Chen
Bone marrow derived mesenchymal stem cells (MSCs) play a critical role in immune modulation. However, immunomodulatory function of whole human umbilical cord derived mesenchymal stem cells (UC-MSCs) remains unclear. In this study, UC-MSCs were separated from whole umbilical cord using a single enzyme digestion. UC-MSCs (CD73(+), CD90(+), CD105(+), and CD34(-), CD45(-), HLA-DR(-)) were differentiated into adipocytes, osteocytes and chondrocytes in vitro under specific stimulatory environments. UC-MSCs suppressed umbilical cord blood lymphocyte proliferation stimulated by mitogen, and ELISA showed that the secretion of INF-γ was downregulated, and the secretion of IL-4 was upregulated, with CD8(+) T cells markedly decreased and CD4(+) T cells changed lightly...
May 19, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28529022/effect-of-microgravity-on-the-mesenchymal-stem-cell-characteristics-of-limbal-fibroblasts
#7
Shu-I Pao, Ke-Hung Chien, Hsin-Ting Lin, Ming-Cheng Tai, Jiann-Torng Chen, Chang-Min Liang
BACKGROUND: Mesenchymal stem cells (MSCs) are important for regenerative medicine. Limbal fibroblasts (LFs), present in the corneal limbus, have been shown to possess MSC characteristics, and can differentiate into other cell types. The current study sought to investigate the effect of microgravity on the proliferation and differentiation of LFs, and identify culture conditions to obtain a high proportion of LFs possessing MSC characteristics. METHODS: A rotary cell culture system was used to generate microgravity...
May 18, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28526089/adipose-derived-mesenchymal-stem-cells-from-liposuction-and-resected-fat-are-feasible-sources-for-regenerative-medicine
#8
Sandra Schneider, Marina Unger, Martijn van Griensven, Elizabeth R Balmayor
BACKGROUND: The use of mesenchymal stem cells (MSCs) in research and in regenerative medicine has progressed. Bone marrow as a source has drawbacks because of subsequent morbidities. An easily accessible and valuable source is adipose tissue. This type of tissue contains a high number of MSCs, and obtaining higher quantities of tissue is more feasible. Fat tissue can be harvested using different methods such as liposuction and resection. First, a detailed isolation protocol with complete characterization is described...
May 19, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/28524929/xeno-free-cultivation-of-mesenchymal-stem-cells-from-the-corneal-stroma
#9
Steffi Matthyssen, Sorcha Ní Dhubhghaill, Veerle Van Gerwen, Nadia Zakaria
Purpose: The human cornea has recently been described as a source of corneal stroma-derived mesenchymal stem cells (hMSCs). In vitro expansion of these cells involves basal medium supplemented with fetal bovine serum (FBS). As animal-derived serum can confer a risk of disease transmission and can be subject to considerable lot-to-lot variability, it does not comply with newer Good Manufacturing Practice (GMP)-required animal component-free culture protocols for clinical translation. Methods: This study investigated animal-free alternatives to FBS for cultivation of human corneal stromal MSCs...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28511664/yields-and-chondrogenic-potential-of-primary-synovial-mesenchymal-stem-cells-are-comparable-between-rheumatoid-arthritis-and-osteoarthritis-patients
#10
Yuji Kohno, Mitsuru Mizuno, Nobutake Ozeki, Hisako Katano, Keiichiro Komori, Shizuka Fujii, Koji Otabe, Masafumi Horie, Hideyuki Koga, Kunikazu Tsuji, Mikio Matsumoto, Haruka Kaneko, Yuji Takazawa, Takeshi Muneta, Ichiro Sekiya
BACKGROUND: Mesenchymal stem cells derived from the synovial membrane (synovial MSCs) are a candidate cell source for regenerative medicine of cartilage and menisci due to their high chondrogenic ability. Regenerative medicine can be expected for RA patients with the inflammation well-controlled as well as OA patients and transplantation of synovial MSCs would also be a possible therapeutic treatment. Some properties of synovial MSCs vary dependent on the diseases patients have, and whether or not the pathological condition of RA affects the chondrogenesis of synovial MSCs remains controversial...
May 16, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28510634/autologous-adipose-derived-tissue-matrix-part-i-biologic-characteristics
#11
Stephen A Schendel
Background: Autologous collagen is an ideal soft tissue filler and may serve as a matrix for stem cell implantation and growth. Procurement of autologous collagen has been limited, though, secondary to a sufficient source. Liposuction is a widely performed and could be a source of autologous collagen. Objectives: The amount of collagen and its composition in liposuctioned fat remains unknown. The purpose of this research was to characterize an adipose-derived tissue-based product created using ultrasonic cavitation and cryo-grinding...
May 16, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28509320/premature-fetal-tissues-are-possible-source-of-valuable-mesenchymal-stem-cells
#12
Filip Andrzej Dąbrowski, Anna Burdzinska, Agnieszka Kulesza, Beata Kaleta, Leszek Pączek, Mirosław Wielgoś
OBJECTIVES: Comparison of the ability to inhibit alloactivated lymphocytes proliferation of human Wharton Jelly (WJ) and amniotic membrane (AM) mesenchymal stem cells (MSCs) from preterm and term pregnancies. MATERIAL AND METHODS: Term-WJ-MSCs (n = 5) and Preterm-WJ-MSCs (n = 1) were obtained from tissue explants by adherent method. Term-AM-MSCs (n = 5) and Preterm-AM-MSCs (n = 1) were obtained by tripsin and collagenase digestion method. Term and Preterm MSCs phenotype was confirmed in vitro by flow cytometry...
2017: Ginekologia Polska
https://www.readbyqxmd.com/read/28499650/the-mesenchymal-state-predicts-poor-disease-free-survival-in-resectable-non-small-cell-lung-cancer
#13
Kunal Mehta, Erika Moravcikova, David McFall, James D Luketich, Arjun Pennathur, Albert D Donnenberg, Vera S Donnenberg
BACKGROUND: The epithelial-mesenchymal transition (EMT) is thought to contribute to the overall invasiveness of malignant cells. Expression of cluster of differentiation (CD) 44 and CD90 mark the mesenchymal state in multiple epithelial malignancies. Their role in lung cancer remains unclear, however. This study evaluated the prognostic significance of CD44 and CD90 coexpression in patients with resectable primary non-small cell lung cancer (NSCLC). METHODS: This was a nonconcurrent cohort study of patients with resectable NSCLC, stratified by the degree of expression of CD44/CD90 double-positive cells in their primary tumor...
May 9, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28499457/preconditioning-of-bone-marrow-derived-mesenchymal-stromal-cells-by-tetramethylpyrazine-enhances-cell-migration-and-improves-functional-recovery-after-focal-cerebral-ischemia-in-rats
#14
Lin Li, Lisheng Chu, Yan Fang, Yan Yang, Tiebing Qu, Jianping Zhang, Yuanjun Yin, Jingjing Gu
BACKGROUND: Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) is one of the new therapeutic strategies for treating ischemic stroke. However, the relatively poor migratory capacity of BMSCs toward infarcted regions limited the therapeutic potential of this approach. Pharmacological preconditioning can increase the expression of CXC chemokine receptor 4 (CXCR4) in BMSCs and enhance cell migration toward the injury site. In the present study, we investigated whether tetramethylpyrazine (TMP) preconditioning could enhance BMSCs migration to the ischemic brain and improve functional recovery through upregulating CXCR4 expression...
May 12, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28499402/erk-signaling-is-required-for-vegf-a-vegfr2-induced-differentiation-of-porcine-adipose-derived-mesenchymal-stem-cells-into-endothelial-cells
#15
Sami G Almalki, Devendra K Agrawal
BACKGROUND: Cell-based therapy that can rejuvenate the endothelium with stimulated adipose-derived mesenchymal stem cells (AMSCs) is a promising therapeutic strategy for the re-endothelialization of denuded arteries at the stenting site. Previously, we have shown that silencing of MMP-2 and MMP-14 inhibits vascular endothelial growth factor receptor type 2 (VEGFR2) cleavage, and induces differentiation of AMSCs toward the endothelial cell (EC) lineage. In this study, we examined the underlying signaling pathways that regulate differentiation of AMSCs to ECs in vitro through VEGFR2...
May 12, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28498335/the-selective-centrifugation-ensures-a-better-in-vitro-isolation-of-ascs-and-restores-a-soft-tissue-regeneration-in-vivo
#16
Francesco De Francesco, Antonio Guastafierro, Gianfranco Nicoletti, Sergio Razzano, Michele Riccio, Giuseppe A Ferraro
Autologous fat grafting procedures in plastic surgery have been extensively used to reinforce soft tissue in congenital or acquired tissue impairments. With this background, the aim of this study is firstly to examine the impact of a selective centrifugation on existing adipose stem cells (ASCs) in terms of stemness profile maintenance and, secondly, to investigate the effect of restoring volume in reconstruction on patients affected by soft tissue damage. After centrifugation, the fat graft products were separated into two layers and subsequently examined in vitro for the expression of CD34, CD90, CD117, CD105, CD29, CD31, CD44, CD73, CD133, CD14 and CD45 markers by flow cytometry and gene expression analyses were performed for Sox2, WNT3A, END, CD44, FUT4, COLL1, CTNNB1, hbEGF, KRTLG, MMP2 and VIM genes...
May 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28494459/influence-of-bovine-serum-lipids-and-fetal-bovine-serum-on-the-expression-of-cell-surface-markers-in-cultured-bovine-preadipocytes
#17
Mansur A Sandhu, Sandra Jurek, Susanne Trappe, Martin Kolisek, Gerhard Sponder, Jörg R Aschenbach
To establish the influence of fetal bovine serum (FBS) and bovine serum lipids (BSL) on cell differentiation marker expression, bovine adipose-derived stem cells from subcutaneous tissue were incubated for 14 days in 4 types of differentiation media containing 10% FBS and 10 µL/mL BSL (TRT-1), no FBS and 10 µL/mL of BSL (TRT-2), 10% FBS and no BSL (TRT-3), or no supplements (TRT-4). Cells were subjected to Nile red staining, immunocytochemistry (CD73, CD90, CD105, DLK1, FabP4), and quantitative real-time PCR (CD73, CD90, CD105, FabP4)...
May 12, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28491094/immunoprofiling-of-adult-derived-human-liver-stem-progenitor-cells-impact-of-hepatogenic-differentiation-and-inflammation
#18
Hoda El-Kehdy, Camillo Sargiacomo, Mohammad Fayyad-Kazan, Hussein Fayyad-Kazan, Catherine Lombard, Laurence Lagneaux, Etienne Sokal, Mehdi Najar, Mustapha Najimi
Adult-derived human liver stem/progenitor cells (ADHLSCs) are, nowadays, developed as therapeutic medicinal product for the treatment of liver defects. In this study, the impact of hepatogenic differentiation and inflammation priming on the ADHLSCs' immune profile was assessed in vitro and compared to that of mature hepatocytes. The constitutive immunological profile of ADHLSCs was greatly different from that of hepatocytes. Differences in the expression of the stromal markers CD90 and CD105, adhesion molecules CD44 and CD49e, immunoregulatory molecules CD73 and HO-1, and NK ligands CD112 and CD155 were noted...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28487975/decreased-phosphorylation-of-pdgfr-%C3%AE-impairs-the-angiogenic-potential-of-expanded-endothelial-progenitor-cells-via-the-inhibition-of-pi3k-akt-signaling
#19
Haiyuan Lu, Hua Mei, Fan Wang, Qian Zhao, Siqi Wang, Lvjun Liu, Lamei Cheng
Human umbilical cord blood-derived endothelial progenitor cells (EPCs) have been proven to contribute to post-natal angiogenesis, and have been applied in various models of ischemia. However, to date, to the best of our knowledge, there is no available data on the angiogenic properties of EPCs during the process of in vitro expansion. In this study, we expanded EPCs to obtain cells at different passages, and analyzed their cellular properties and angiogenic ability. In the process of expansion, no changes were observed in cell cobblestone-like morphology, apoptotic rate and telomere length...
June 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28482443/-effects-of-cell-to-cell-communication-and-histone-acetyltransferase-on-the-change-of-osteogenic-differentiation-ability-among-single-cell-clones-from-healthy-periodontium-with-heterogeneity-of-osteogenic-differentiation-abilities
#20
D D Fei, B Li, F Gao, A Q Liu, Y Jin, Q T Wang
Objective: To investigate the effect of cell-to-cell communication amongst single-cell clones from healthy periodontium with different osteogenic differentiation potentials on change of osteogenic differentiation capabilities and the role histone acetyltransferase partaken in this process. Methods: In order to research the change of osteogenic differentiation ability via cell-to-cell communication, indirect co-culture method was used by placing two single-cell clones with different osteogenesis potentials in each of the 6-well plates...
May 9, 2017: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
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