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https://www.readbyqxmd.com/read/28334693/fabrication-of-human-hair-keratin-jellyfish-collagen-eggshell-derived-hydroxyapatite-osteoinductive-biocomposite-scaffolds-for-bone-tissue-engineering-from-waste-to-regenerative-medicine-products
#1
Yavuz Emre Arslan, Tugba Sezgin Arslan, Burak Derkus, Emel Emregul, Kaan C Emregul
In the present study, we aimed at fabricating an osteoinductive biocomposite scaffold using keratin obtained from human hair, jellyfish collagen and eggshell-derived nano-sized spherical hydroxyapatite (nHA) for bone tissue engineering applications. Keratin, collagen and nHA were characterized with the modified Lowry method, free-sulfhydryl groups and hydroxyproline content analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) and thermal gravimetric analysis (TGA) which confirmed the success of the extraction and/or isolation processes...
March 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28329833/thy1-cd90-expression-is-reduced-by-the-environmental-chemical-tetrabromobisphenol-a-to-promote-adipogenesis-through-induction-of-microrna-103
#2
Collynn F Woeller, E'Lissa Flores, Stephen J Pollock, Richard P Phipps
Environmental chemicals termed "obesogens" disrupt the endocrine system to promote adipogenesis and obesity. Tetrabromobisphenol-A (TBBPA) has been reported to increase adipogenesis, however, the mechanism(s) of action are unclear. Thy1 (CD90) is a glycophosphatidylinositol (GPI) -anchored membrane protein that serves as a marker for stem cells and also plays an important role in regulating adipogenesis and obesity. We investigated whether or not TBBPA promotes adipogenesis in human and mouse cells by reducing Thy1 levels...
February 22, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28325116/stem-cell-markers-describe-a-transition-from-somatic-to-pluripotent-cell-states-in-a-rat-model-of-endometriosis
#3
Essam Rashad Othman, Fatma Yassine Meligy, Ayat Abdel-Rahman Sayed, Mohamed Ahmed El-Mokhtar, Abeer Mohamed Refaiy
OBJECTIVE: To study Thy1 as a fibroblast marker, SSEA1 as a marker of intermediate pluripotency, and Oct4 as a marker of established pluripotency in rat model of endometriosis. DESIGN: In vivo animal study. MATERIALS AND METHODS: Endometriosis was induced in 20 albino female rats through autologous transplantation of one uterine horn to mesentery of intestine. Other 20 rats had their horn removed without transplantation (controls). Rats were sacrificed 4 weeks after induction surgery...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28323901/characterization-of-stromal-vascular-fraction-and-adipose-stem-cells-from-subcutaneous-preperitoneal-and-visceral-morbidly-obese-human-adipose-tissue-depots
#4
Karina Ribeiro Silva, Isis Côrtes, Sally Liechocki, João Regis Ivar Carneiro, Antônio Augusto Peixoto Souza, Radovan Borojevic, Clarissa Menezes Maya-Monteiro, Leandra Santos Baptista
BACKGROUND/OBJECTIVES: The pathological condition of obesity is accompanied by a dysfunctional adipose tissue. We postulate that subcutaneous, preperitoneal and visceral obese abdominal white adipose tissue depots could have stromal vascular fractions (SVF) with distinct composition and adipose stem cells (ASC) that would differentially account for the pathogenesis of obesity. METHODS: In order to evaluate the distribution of SVF subpopulations, samples of subcutaneous, preperitoneal and visceral adipose tissues from morbidly obese women (n = 12, BMI: 46...
2017: PloS One
https://www.readbyqxmd.com/read/28321780/isolation-culture-and-identification-of-pulmonary-arterial-smooth-muscle-cells-from-rat-distal-pulmonary-arteries
#5
Gongyong Peng, Juan Xu, Rongmin Liu, Zhenli Fu, Shaoxing Li, Wei Hong, Jinglong Chen, Bing Li, Pixin Ran
The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized protocol for easy isolation and culture of PASMCs from rat distal PA to supply every investigator with a simple, economical and useful method in studying PH. The protocol can be divided into four stages: isolation of distal PA, isolation of cells, growth in culture and passage of cells...
March 21, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28320452/compared-to-the-amniotic-membrane-wharton-s-jelly-may-be-a-more-suitable-source-of-mesenchymal-stem-cells-for-cardiovascular-tissue-engineering-and-clinical-regeneration
#6
Lei Pu, Mingyao Meng, Jian Wu, Jing Zhang, Zongliu Hou, Hui Gao, Hui Xu, Boyu Liu, Weiwei Tang, Lihong Jiang, Yaxiong Li
BACKGROUND: The success of developing cardiovascular tissue engineering (CTE) grafts greatly needs a readily available cell substitute for endothelial and interstitial cells. Perinatal annexes have been proposed as a valuable source of mesenchymal stem cells (MSCs) for tissue engineering and regenerative medicine. The objective of the present study is to evaluate the potential of human Wharton's jelly MSCs (WJ-MSCs) and amniotic membrane MSCs (AM-MSCs) as a seeding cell in CTE and cardiovascular regenerative medicine...
March 21, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28319827/immunophenotypical-characterization-of-canine-mesenchymal-stem-cells-from-perivisceral-and-subcutaneous-adipose-tissue-by-a-species-specific-panel-of-antibodies
#7
Ana Ivanovska, Stefano Grolli, Paolo Borghetti, Francesca Ravanetti, Virna Conti, Elena De Angelis, Francesca Macchi, Roberto Ramoni, Paolo Martelli, Ferdinando Gazza, Antonio Cacchioli
Immunophenotypical characterization of mesenchymal stem cells is fundamental for the design and execution of sound experimental and clinical studies. The scarce availability of species-specific antibodies for canine antigens has hampered the immunophenotypical characterization of canine mesenchymal stem cells (MSC). The aim of this study was to select a panel of species-specific direct antibodies readily useful for canine mesenchymal stem cells characterization. They were isolated from perivisceral and subcutaneous adipose tissue samples collected during regular surgeries from 8 dogs...
February 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28303135/a-human-lin-cd123-cd127-low-population-endowed-with-ilc-features-and-migratory-capabilities-contributes-to-immunopathological-hallmarks-of-psoriasis
#8
Luz María Mora-Velandia, Octavio Castro-Escamilla, Andrés González Méndez, Cristina Aguilar-Flores, Martha Velázquez-Avila, María Isabel Tussié-Luna, Juan Téllez-Sosa, César Maldonado-García, Fermín Jurado-Santacruz, Eduardo Ferat-Osorio, Jesus Martínez-Barnetche, Rosana Pelayo, Laura C Bonifaz
Innate lymphoid cells (ILC) are members of a heterogeneous family with a lymphoid origin that mimics the T helper (Th) cytokine profile. ILC are involved in early effector cytokine-mediated responses during infections in peripheral tissues. ILC also play an important role in chronic skin inflammatory diseases, including psoriasis. Although classical ILC express CD127, it has been recently reported that the presence of non-classical CD127(-) ILC populations and an early ILC precursor (EILP) CD127(low). ILC development has predominately been investigated in mouse models...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28301220/periostin-promotes-migration-proliferation-and-differentiation-of-human-periodontal-ligament-mesenchymal-stem-cells
#9
Ziqiang Wu, Wenyong Dai, Pei Wang, Xiaozhen Zhang, Yi Tang, Lin Liu, Qiaona Wang, Ming Li, Chunbo Tang
PURPOSE: Periostin (POSTN) is critical to bone and dental tissue morphogenesis, postnatal development, and maintenance, however, its roles in tissue repair and regeneration mediated by human periodontal ligament mesenchymal stem cells (PDLSCs) remain unclear. The present study was designed to evaluate the effects of POSTN on hPDLSCs in vitro. MATERIALS AND METHODS: hPDLSCs were isolated and characterized by their expression of the cell surface markers CD44, CD90, CD105, CD34, and CD45...
March 16, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28296006/intracoronary-allogeneic-cardiosphere-derived-stem-cells-are-safe-for-use-in-dogs-with-dilated-cardiomyopathy
#10
Michael Taylor Hensley, Junnan Tang, Kathleen Woodruff, Teresa Defrancesco, Sandra Tou, Christina M Williams, Mathew Breen, Kathryn Meurs, Bruce Keene, Ke Cheng
Cardiosphere-derived cells (CDCs) have been shown to reduce scar size and increase viable myocardium in human patients with mild/moderate myocardial infarction. Studies in rodent models suggest that CDC therapy may confer therapeutic benefits in patients with non-ischaemic dilated cardiomyopathy (DCM). We sought to determine the safety and efficacy of allogeneic CDC in a large animal (canine) model of spontaneous DCM. Canine CDCs (cCDCs) were grown from a donor dog heart. Similar to human CDCs, cCDCs express CD105 and are slightly positive for c-kit and CD90...
March 15, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28295953/three-dimensional-carbon-nanotube-scaffolds-for-long-term-maintenance-and-expansion-of-human-mesenchymal-stem-cells
#11
Gaurav Lalwani, Michael D'Agati, Anu Gopalan, Sunny C Patel, Yahfi Talukdar, Balaji Sitharaman
Expansion of mesenchymal stem cells (MSCs) and maintenance of their self-renewal capacity in vitro requires specialized robust cell culture systems. Conventional approaches using animal derived or artificial matrices and a cocktail of growth factors have limitations such as consistency, scalability, pathogenicity and loss of MSC phenotype. Herein, we report the use of all-carbon 3D single- and multiwalled carbon nanotube scaffolds (SWCNTs and MWCNTs) as artificial matrices for long-term maintenance and expansion of human MSCs...
March 10, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28295901/high-aldehyde-dehydrogenase-activity-identifies-a-subset-of-human-mesenchymal-stromal-cells-with-vascular-regenerative-potential
#12
Stephen E Sherman, Miljan Kuljanin, Tyler T Cooper, David M Putman, Gilles A Lajoie, David A Hess
During culture expansion, multipotent mesenchymal stromal cells (MSC) differentially express aldehyde dehydrogenase (ALDH), an intracellular detoxification enzyme that protects long-lived cells against oxidative stress. Thus, MSC selection based on ALDH-activity may be used to reduce heterogeneity and distinguish MSC subsets with improved regenerative potency. After expansion of human bone marrow-derived MSC, cell progeny were purified based on low versus high ALDH-activity by fluorescence activated cell sorting, and each subset was compared for multipotent stromal and pro-vascular regenerative functions...
March 15, 2017: Stem Cells
https://www.readbyqxmd.com/read/28286160/p2x7-receptor-promotes-intestinal-inflammation-in-chemically-induced-colitis-and-triggers-death-of-mucosal-regulatory-t-cells
#13
Vanessa R Figliuolo, Luiz Eduardo Baggio Savio, Hanaa Safya, Hayandra Nanini, Cláudio Bernardazzi, Alessandra Abalo, Heitor S P de Souza, Jean Kanellopoulos, Pierre Bobé, Cláudia M L M Coutinho, Robson Coutinho-Silva
P2X7 receptor activation contributes to inflammation development in different pathologies. We previously reported that the P2X7 receptor is over-expressed in the gut mucosa of patients with inflammatory bowel disease, and that P2X7 inhibition protects against chemically induced colitis. Here, we investigated in detail the role of the P2X7 receptor in inflammatory bowel disease development, by treating P2X7 knockout (KO) and WT mice with two different (and established) colitis inductors. P2X7 KO mice were protected against gut inflammation induced by 2,4,6-trinitrobenzenesulfonic acid or oxazolone, with no weight loss or gut histological alterations after treatment...
March 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28284665/cryopreservation-and-multipotential-characteristics-evaluation-of-a-novel-type-of-mesenchymal-stem-cells-derived-from-small-tailed-han-sheep-fetal-lung-tissue
#14
Caiyun Ma, Changqing Liu, Xiangchen Li, Taofeng Lu, Chunyu Bai, Yanan Fan, Weijun Guan, Yu Guo
Lung mesenchymal stem cells (L-MSCs) characterized by plasticity, reduced relative immune privilege and high anti-fibrosis characteristics play the crucial role in lung tissue regenerative processes. However, up to date, the multi-differentiation potentials and application values of L-MSCs are still uncertain. In the current study, the Small Tailed Han Sheep embryo L-MSCs line from 12 samples, stocking 124 cryogenically-preserved vials, was successfully established by using primary culture and cell cryopreservation techniques...
March 8, 2017: Cryobiology
https://www.readbyqxmd.com/read/28281780/fibroblast-growth-factor-1-transfected-adipose-derived-mesenchymal-stem-cells-promote-angiogenic-proliferation
#15
Seyed Javad Hoseini, Hamed Ghazavi, Fatemeh Forouzanfar, Baratali Mashkani, Ahmad Ghorbani, Elahe Mahdipour, Faezeh Ghasemi, Hamid Reza Sadeghnia, Majid Ghayour-Mobarhan
The aim of this study was to investigate, for the first time, the effects of using adipose-derived mesenchymal stem cells (AD-MSCs) transfected with an episomal plasmid encoding fibroblast growth factor 1 (FGF1) (AD-MSCs(FGF1)), in providing the microenvironment required for angiogenic proliferation. The isolated rat AD-MSCs were positive for mesenchymal (CD29 and CD90) and negative for hematopoietic (CD34 and CD45) surface markers. Adipogenic and osteogenic differentiation of the AD-MSCs also occurred in the proper culture media...
March 10, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28281185/sex-and-age-as-determinants-of-rat-t-cell-phenotypic-characteristics-influence-of-peripubertal-gonadectomy
#16
Nevena Arsenović-Ranin, Duško Kosec, Ivan Pilipović, Mirjana Nacka-Aleksić, Biljana Bufan, Zorica Stojić-Vukanić, Gordana Leposavić
The study examined the influence of age, sex and peripubertal gonadectomy on a set of T-cell phenotypic parameters. Rats of both sexes were gonadectomised at the age of 1 month and peripheral blood and spleen T lymphocytes from non-gonadectomised and gonadectomised 3- and 11-month-old rats were examined for the expression of differentiation/activation (CD90/CD45RC) and immunoregulatory markers. Peripheral blood T lymphocytes from non-gonadectomised rats showed age-dependent sexual dimorphisms in (1) total count (lower in female than male 11-month-old rats); (2) CD4+:CD8 + cell ratio (higher in female than male rats of both ages); (3) the proportion of recent thymic emigrants in CD8 + T cells (lower in female than male 3-month-old rats) and (4) the proportions of mature naïve and memory/activated cells (irrespective of age, the proportion of naïve cells was higher, whereas that of memory/activated cells was lower in females)...
March 9, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28276486/a-potential-regulatory-network-underlying-distinct-fate-commitment-of-myogenic-and-adipogenic-cells-in-skeletal-muscle
#17
Wenjuan Sun, Ting He, Chunfu Qin, Kai Qiu, Xin Zhang, Yanhong Luo, Defa Li, Jingdong Yin
Mechanism controlling myo-adipogenic balance in skeletal muscle is of great significance for human skeletal muscle dysfunction and myopathies as well as livestock meat quality. In the present study, two cell subpopulations with particular potency of adipogenic or myogenic differentiation were isolated from neonatal porcine longissimus dorsi using the preplate method to detect mechanisms underlying distinct fate commitment of myogenic and adipogenic cells in skeletal muscle. Both cells share a common surface expression profile of CD29(+)CD31(-)CD34(-)CD90(+)CD105(+), verifying their mesenchymal origin...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28274315/-vegf-overespression-promotes-the-proliferation-and-differentiation-of-human-adipose-derived-stem-cells
#18
Youbai Chen, Qixu Zhang, E Butler Charles, Yewen Wu, Liping Zhang, Jiangling Dong, Yan Han
Objective To investigate the effect of vascular endothelial growth factor (VEGF) overexpression on the phenotype, proliferation and multilineage differentiation ability of human adipose-derived stem cells (hADSCs). Methods Passage-2 hADSCs were transfected with pIRES2-EGFP-VEGF plasmid using DOTAP liposomal technique, whereas hADSCs in control group were transfected in absence of plasmid. The success of transfection was confirmed by immunofluorescent cytochemistry. Phenotype differences between the two groups were detected using flow cytometry...
March 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28262465/characterization-of-human-fibroblastic-reticular-cells-as-potential-immunotherapeutic-tools
#19
Jaris Valencia, Eva Jiménez, Víctor G Martínez, Beatriz G Del Amo, Laura Hidalgo, Ana Entrena, Lidia M Fernández-Sevilla, Francisco Del Río, Alberto Varas, Ángeles Vicente, Rosa Sacedón
Fibroblastic reticular cells (FRCs) are essential players during adaptive immune responses not only as a structural support for the encounter of antigen-presenting cells and naive T lymphocytes but also as a source of modulatory signals. However, little is known about this cell population in humans. To address the phenotypical and functional analysis of human FRCs here we established splenic (SP) and mesenteric lymph node (LN) CD45(-)CD31(-)CD90(+)podoplanin(+) myofibroblastic cell cultures. They shared the phenotypical characteristics distinctive of FRCs, including the expression of immunomodulatory factors and peripheral tissue antigens...
March 2, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28259258/optimal-culture-conditions-are-critical-for-efficient-expansion-of-human-testicular-somatic-and-germ-cells-in%C3%A2-vitro
#20
Itai Gat, Leila Maghen, Melissa Filice, Brandon Wyse, Khaled Zohni, Keith Jarvi, Kirk C Lo, Andrée Gauthier Fisher, Clifford Librach
OBJECTIVE: To optimize culture conditions for human testicular somatic cells (TSCs) and spermatogonial stem cells. DESIGN: Basic science study. SETTING: Urology clinic and stem cell research laboratory. PATIENT(S): Eight human testicular samples. INTERVENTIONS(S): Testicular tissues were processed by mechanical and enzymatic digestion. Cell suspensions were subjected to differential plating (DP) after which floating cells (representing germ cells) were removed and attached cells (representing TSCs) were cultured for 2 passages (P0-P1) in StemPro-34- or DMEM-F12-based medium...
March 2017: Fertility and Sterility
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