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https://www.readbyqxmd.com/read/28746279/thy1-cd90-expression-is-elevated-in-radiation-induced-periprosthetic-capsular-contracture-implication-for-novel-therapeutics
#1
Trevor C Hansen, Collynn F Woeller, Shannon H Lacy, Peter F Koltz, Howard N Langstein, Richard P Phipps
BACKGROUND: Capsular contracture is a devastating complication of postmastectomy implant-based breast reconstruction. Unfortunately, capsular contracture rates are drastically increased by targeted radiotherapy, a standard postmastectomy treatment. Thy1 (also called CD90) is important in myofibroblast differentiation and scar tissue formation. However, the impact of radiotherapy on Thy1 expression and the role of Thy1 in capsular contracture are unknown. METHODS: The authors analyzed Thy1 expression in primary human capsular tissue and primary fibroblast explants by real-time quantitative polymerase chain reaction, Western blotting, and immunohistochemistry...
August 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28744021/thy-1-cancer-associated-fibroblasts-adversely-impact-lung-cancer-prognosis
#2
Mark J Schliekelman, Chad J Creighton, Brandi N Baird, Yulong Chen, Priyam Banerjee, Neus Bota-Rabassedas, Young-Ho Ahn, Jonathon D Roybal, Fengju Chen, Yiqun Zhang, Dhruva K Mishra, Min P Kim, Xin Liu, Barbara Mino, Pamela Villalobos, Jaime Rodriguez-Canales, Carmen Behrens, Ignacio I Wistuba, Samir M Hanash, Jonathan M Kurie
Cancer-associated fibroblasts (CAFs) regulate diverse intratumoral biological programs and can promote or inhibit tumorigenesis, but those CAF populations that negatively impact the clinical outcome of lung cancer patients have not been fully elucidated. Because Thy-1 (CD90) marks CAFs that promote tumor cell invasion in a murine model of Kras(G12D)-driven lung adenocarcinoma (Kras(LA1)), here we postulated that human lung adenocarcinomas containing Thy-1(+) CAFs have a worse prognosis. We first examined the location of Thy-1(+) CAFs within human lung adenocarcinomas...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28743889/limbal-stromal-cells-derived-from-porcine-tissue-demonstrate-mesenchymal-characteristics-in-vitro
#3
Julia Fernández-Pérez, Marcus Binner, Carsten Werner, Laura J Bray
Limbal stromal cells (LSCs) from the human ocular surface display mesenchymal stromal cell characteristics in vitro. In this study, we isolated cells from the porcine limbal stroma (pLSCs), characterised them, and evaluated their ability to support angiogenesis and the culture of porcine limbal epithelial stem cells (pLESCs). The isolated cells adhered to plastic and grew in monolayers in vitro using serum-supplemented or serum-free medium. The pLSCs demonstrated expression of CD29, and cross-reactivity with anti-human CD45, CD90, CD105, CD146, and HLA-ABC...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28740514/normal-versus-pathological-cardiac-fibroblast-derived-extracellular-matrix-differentially-modulates-cardiosphere-derived-cell-paracrine-properties-and-commitment
#4
Francesca Pagano, Francesco Angelini, Clotilde Castaldo, Vittorio Picchio, Elisa Messina, Sebastiano Sciarretta, Ciro Maiello, Giuseppe Biondi-Zoccai, Giacomo Frati, Franca di Meglio, Daria Nurzynska, Isotta Chimenti
Human resident cardiac progenitor cells (CPCs) isolated as cardiosphere-derived cells (CDCs) are under clinical evaluation as a therapeutic product for cardiac regenerative medicine. Unfortunately, limited engraftment and differentiation potential of transplanted cells significantly hamper therapeutic success. Moreover, maladaptive remodelling of the extracellular matrix (ECM) during heart failure progression provides impaired biological and mechanical signals to cardiac cells, including CPCs. In this study, we aimed at investigating the differential effect on the phenotype of human CDCs of cardiac fibroblast-derived ECM substrates from healthy or diseased hearts, named, respectively, normal or pathological cardiogel (CG-N/P)...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28728289/-the-protective-effect-of-pigment-epithelial-derived-factor-modified-human-umbilical-cord-mesenchymal-stem-cells-on-rats-with-diabetic-retinopathy
#5
W Zhang, H T Duan, S Chen, Y X Wang, J H Kong, M Dong, X Bi, J Song
Objective: To investigate the effect of pigment epithelial-derived factor (PEDF) gene-modified human umbilical cord mesenchymal stem cells (MSC) on rats with diabetic retinopathy (DR). Methods: Experimental study. Human umbilical cord MSC were transfected by lentivirus packaging PEDF-MSC-green fluorescent protein (GFP) and GFP-MSC plasmid vectors, and the expression of PEDF and vascular endothelial growth factor (VEGF) was measured in the cell culture medium. Fifty adult male Sprague-Dawley rats were randomly divided into five groups: normal control group (group A), DR control group (group B), phosphate-buffered saline (PBS) treated group (group C), GFP-MSC treated group (group D) and PEDF-MSC-GFP treated group (group E), with 10 rats in each group...
July 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28726209/characteristics-of-mel-ibr-melanoma-line-subclone-after-treatment-with-chicken-embryo-extract
#6
N M Suraeva, L F Morozova, O O Ryabaya, Yu A Khochenkova, A V Samoilov, O S Burova, N V Golubtsova, A E Barmashov, M A Baryshnikova
We continue analysis of the phenotype of human melanoma cell Mel Ibr subclone obtained previously by treatment of the parental cell line by chicken embryo extract. The present study is focused on detection of markers of epithelial-mesenchymal transition that determine enhanced metastatic and invasive potential of malignant tumors of various locations. Analysis of the expression of E-cadherin and vimentin genes in the subclone and parental cells detected activation of epithelial-mesenchymal transition in the subclone...
June 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726193/regenerative-potential-of-spermatogonial-stem-cells-endothelial-progenitor-cells-and-epithelial-progenitor-cells-of-c57bl-6-male-mice-with-metabolic-disorders
#7
E G Skurikhin, A V Pakhomova, O V Pershina, L A Ermolaeva, V A Krupin, N N Ermakova, E S Pan, A I Kudryashova, O Yu Rybalkina, T B Pavlovskaya, N V Litvyakov, V E Goldberg, A M Dygai
The properties of spermatogonial stem cells, endothelial progenitor cells, and the epithelial progenitors of C57Bl/6 mice under conditions of metabolic disorders were studied using the model of busulfan-induced suppression of spermatogenesis and in vitro culture technique. Spermatogonial stem cells CD117-CD90(+) and epithelial progenitors CD45-CD31-Sca-1(+)CD49f(+) derived from the testes of mice with metabolic disturbances demonstrated 17- and 28-fold increase in the respective cell mass and generated cell colonies in vitro...
June 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28724609/thy-1-fibroblast-subsets-in-the-human-peritoneum
#8
Edyta Kawka, Janusz Witowski, Maria Bartosova, Rusan Catar, Andras Rudolf, Aurelie Philippe, Rafal Rutkowski, Betti Schaefer, Claus Peter Schmitt, Duska Dragun, Achim Joerres
Fibrotic thickening of the peritoneum develops in patients receiving peritoneal dialysis (PD) for renal failure. For unknown reasons, however, in some patients it progresses to extensive fibrosis that compromises dialysis capacity of the peritoneum. It is increasingly clear that fibroblasts display large heterogeneity not only between but also within tissues. Differential surface expression of glycoprotein Thy-1 (CD90) has been shown to identify functionally distinct fibroblast subsets in several organs. Here, we isolated Thy-1+/- subsets of human peritoneal fibroblasts (HPFB) and analyzed them in terms of pro-fibrotic myofibroblast features...
July 19, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28713938/effects-of-age-on-biological-and-functional-characterization-of-adipose%C3%A2-derived-stem-cells-from-patients-with-end%C3%A2-stage-liver-disease
#9
Yanyu Jin, Lei Yang, Yanyan Zhang, Wei Gao, Zhi Yao, Yang Song, Yuliang Wang
Adipose‑derived mesenchymal stromal cells (ADSCs) possess a multilineage potential and immunoregulatory properties, and may have great potential in autologous cell‑based technologies. The aim of the present study was to investigate how the age of patients with benign end‑stage liver disease affected the biological and functional characteristics of ADSCs, which is important for increasing the potential effectiveness of autologous cell therapy. ADSCs were obtained and cultured from three distinct age groups: Infant, adult and elderly...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28711819/differentiation-potential-of-synoviocytes-derived-from-joints-with-cranial-cruciate-ligament-rupture-and-medial-patella-luxation-in-dogs
#10
H M Suranji Wijekoon, Kazuhide Toyota, Sangho Kim, Jing Fang, Eugene C Bwalya, Kenji Hosoya, Masahiro Okumura
The objective of this study was to assess the differentiation capability of synoviocytes derived from dogs with inflammatory joint conditions. Cranial cruciate ligament ruptured (CCLr) (n=12) and medial patella luxated (MPL) (n=10) knee joints of the dogs were used to collect the synovial membrane (SM). Synoviocytes were enzymatically released from the SM and analyzed by flow cytometry for specific cellular markers (CD44 and CD90) of mesenchymal stem cells (MSCs), while doing histopathology from another part of SM sections...
July 9, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28708537/a-proteomic-study-of-mesenchymal-stem-cells-from-equine-umbilical-cord
#11
Leandro Maia, Carolina Nogueira de Moraes, Marianne Camargos Dias, Julia Bauzá Martinez, Antonia Odena Caballol, Giorgia Testoni, Carla Martins de Queiroz, Ramón Díaz Peña, Fernanda C Landim-Alvarenga, Eliandre de Oliveira
To the best of our knowledge, this is the first study describing the proteome of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs) in a global and functional manner. The aim of this work was to analyze the proteome of previously characterized UCIM-MSCs to determine protein abundance and classify the identified proteins according to Gene Ontology (GO) terms. Protein classification analysis according to biological process, molecular function and cellular component was performed using the PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System, which revealed enrichment for 42 biological processes, 23 molecular functions and 18 cellular components...
May 24, 2017: Theriogenology
https://www.readbyqxmd.com/read/28701175/isolation-and-characterization-of-equine-endometrial-mesenchymal-stromal-cells
#12
B Elisabeth Rink, Karin R Amilon, Cristina L Esteves, Hilari M French, Elaine Watson, Christine Aurich, F Xavier Donadeu
BACKGROUND: Equine mesenchymal stromal/stem cells (MSCs) are most commonly harvested from bone marrow (BM) or adipose tissue, requiring the use of surgical procedures. By contrast, the uterus can be accessed nonsurgically, and may provide a more readily available cell source. While human endometrium is known to harbor mesenchymal precursor cells, MSCs have not been identified in equine endometrium. This study reports the isolation, culture, and characterization of MSCs from equine endometrium...
July 12, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28693305/comparison-the-characteristics-of-canine-adipose-tissue-derived-mesenchymal-stem-cells-extracted-from-different-sites-and-at-different-passage-numbers
#13
Kevin M Yaneselli, Cristiana Palma Kuhl, Paula Barros Terraciano, Fernanda de Los Santos de Oliveira, Sabrina Beal Pizzato, Kamila Pazza, Alessandra Bileski Magrisso, Vanessa Torman, Analía Rial, María Moreno, Silvia Llambí, Elizabeth Cirne Lima, Jacqueline Maisonnave
Mesenchymal Stem Cells (MSCs) have attractive characteristics for therapy in animal models and veterinary medicine, due to the capacity of inducing tissue regeneration and immunomodulation. The objective of this study was to evaluate the differences between canine adipose tissue-derived MSCs (AD-MSCs) extracted from subcutaneous (Sc) and visceral (Vs) sites. Surface antigenic markers, in vitro differentiation and mineralized matrix quantification of AD-MSCs in different passages (P4, P6 and P8) were studied...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28690192/suppression-of-osteogenic-differentiation-in-mesenchymal-stem-cells-from-patients-with-ossification-of-the-posterior-longitudinal-ligament-by-a-histamine-2-receptor-antagonist
#14
Xizhe Liu, Gentaro Kumagai, Kanichiro Wada, Toshihiro Tanaka, Taku Fujita, Ayako Sasaki, Ken-Ichi Furukawa, Yasuyuki Ishibashi
Mesenchymal stem cells (MSCs) in ossification of the posterior longitudinal ligament (OPLL) patients have a high propensity toward osteogenesis. Histamine receptor H2 (H2R) antagonists (H2 blockers) like famotidine decrease ossification in patients, by an unclear mechanism. To confirm that MSCs express H2R and to clarify how H2 blockers suppress osteogenic differentiation, we used spinal-ligament MSCs from patients with OPLL or with cervical spondylotic myelopathy (CSM) (control). The MSCs were treated with 10, 30, or 100nM famotidine for 7 or 21 days...
July 8, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28688831/local-chemical-sympathectomy-of-rat-bone-marrow-and-its-effect-on-marrow-cell-composition
#15
P Dubový, I Klusáková, L Kučera, J Osičková, J Chovancová, T Loja, J Mayer, M Doubek, M Joukal
Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naïve femurs...
June 23, 2017: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/28685122/nano-engineered-skin-mesenchymal-stem-cells-potential-vehicles-for-tumour-targeted-quantum-dot-delivery
#16
Liga Saulite, Dominyka Dapkute, Karlis Pleiko, Ineta Popena, Simona Steponkiene, Ricardas Rotomskis, Una Riekstina
Nanotechnology-based drug design offers new possibilities for the use of nanoparticles in imaging and targeted therapy of tumours. Due to their tumour-homing ability, nano-engineered mesenchymal stem cells (MSCs) could be utilized as vectors to deliver diagnostic and therapeutic nanoparticles into a tumour. In the present study, uptake and functional effects of carboxyl-coated quantum dots QD655 were studied in human skin MSCs. The effect of QD on MSCs was examined using a cell viability assay, Ki67 expression analysis, and tri-lineage differentiation assay...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28684895/comparative-study-of-the-methods-of-extracting-mesenchymal-stem-cells-from-cryopreserved-wharton-s-jelly
#17
Peng Yew Kenny Boey, Say Liang Daniel Lim, Kin Fai Tang, Ming Ming Li, Andrew Krishna Ekaputra, Prosanto Kumar Chowdhury, Rajat Anand Gopal Mukherjee, Jennifer Teo, Arvin C Faundo, Yoke Fong Chiew
The Wharton's Jelly (WJ) is an established source of mesenchymal stem cells (MSC). We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods. The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteria: displayed plastic-adherence, co-expressed CD73, CD90, CD105 and were negative for hematopoietic lineage cell markers...
2017: Journal of Stem Cells & Regenerative Medicine
https://www.readbyqxmd.com/read/28678109/age-correlated-phenotypic-alterations-in-cells-isolated-from-human-degenerated-intervertebral-discs-with-contained-hernias
#18
Maria Molinos, Carla Cunha, Catarina R Almeida, Raquel M Gonçalves, Paulo Pereira, Pedro Santos Silva, Rui Vaz, Mário A Barbosa
STUDY DESIGN: Human intervertebral disc (hIVD) cells were isolated from 41 surgically excised samples and assessed for their phenotypic alterations with age. OBJECTIVE: Towards the design of novel anti-ageing strategies to overcome degenerative disc disease (DDD), we investigated age-correlated phenotypic alterations that occur on primary hIVD cells. SUMMARY OF BACKGROUND DATA: Although regenerative medicine holds great hope, much is still to be unveiled on IVD cell biology and its intrinsic signalling pathways, which can lead the way to successful therapies for IDD...
July 3, 2017: Spine
https://www.readbyqxmd.com/read/28677766/human-decidua-mesenchymal-stem-cells-regulate-decidual-natural-killer-cell-function-via-interactions-between-collagen-and-leukocyte%C3%A2-associated-immunoglobulin%C3%A2-like-receptor-1
#19
Qiang Fu, Xuejing Man, Min Yu, Yongli Chu, Xiying Luan, Hailan Piao, Jiangnan Xue, Changzhu Jin
The development of maternal tolerance to the fetal allograft in critical for the maintenance of the pregnancy, and it is accompanied by the development of a special decidual natural killer (dNK) cell tolerance phenotype. To understand the factors that influence dNK cells during early pregnancy, the present study aimed to identify mesenchymal stem cells (MSCs) from human first‑trimester deciduas, termed decidual MSCs (DMSCs), and to investigate the effect of DMSCs on the regulation of dNK cells via collagen...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28677723/telomere-length-is-positively-associated-with-the-expression-of-il%C3%A2-6-and-mip%C3%A2-1%C3%AE-in-bone-marrow-mesenchymal-stem-cells-of-multiple-myeloma
#20
Shengli Li, Yang Jiang, Ai Li, Xiaoli Liu, Xiangling Xing, Yanan Guo, Yaqi Xu, Yunliang Hao, Chengyun Zheng
Potential roles of mesenchymal stem cells (MSCs) in the pathogenesis of multiple myeloma (MM) are largely unknown. In the current study, the authors analyzed telomere length and the expressions of interleukin (IL)‑6 and macrophage inflammatory protein (MIP)‑1α in MSCs derived from the bone marrow (BM) of MM patients and controls. The current results demonstrated that there was no significant difference in cell surface expression of CD73 and CD90, and the capacity to differentiate into bone tissue were identified between the BM MSCs derived from MM patients and controls...
September 2017: Molecular Medicine Reports
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