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https://www.readbyqxmd.com/read/29328479/the-human-umbilical-cord-stem-cells-improve-the-viability-of-oa-degenerated-chondrocytes
#1
Hao Wang, Xu Yan, Yuxin Jiang, Zheng Wang, Yufei Li, Qingdong Shao
Osteoarthritis (OA) affects a large number of patients; however, human umbilical cord stem cells exhibit therapeutic potential for treating OA. The aim of the present study was to explore the interaction between human umbilical cord stem cells and degenerated chondrocytes, and the therapeutic potential of human umbilical cord stem cells on degenerated chondrocytes. Human umbilical cord‑derived mesenchymal stem cells (hUC‑MSCs) were harvested from human umbilical cords, and flow cytometry was used to analyze the surface antigen markers, in addition, chondrogenic, osteogenic and adipogenic differentiation on the cells was investigated...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29327669/advanced-3d-models-culture-to-investigate-mesenchymal-stromal-cells-of-the-human-dental-follicle
#2
Nathalie Steimberg, Francesca Angiero, Davide Farronato, Angiola Berenzi, Gianguido Cossellu, Andrea Ottonello, Darnell Kaigler, Giovanna Mazzoleni
The human dental follicle (hDF) contains the developing tooth and is involved in regulating tooth maturation and eruption. To investigate the mesenchymal stromal cells of the dental follicle, two 3D culture models were used, based on a dynamic bioreactor: the Rotary Cell Culture System (RCCS) and the 3D culture of precursor cells isolated from follicular tissue (hDFCs). The hDFCs were obtained from impacted third molars of 20 patients. Two 3D culture models were tested. In the first model, intact hDF explants were cultured in 3D conditions, preserving the original tissue architecture; they were studied via histomorphological and molecular analyses...
January 12, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29323717/chondrogenic-differentiation-of-human-scalp-adipose-derived-stem-cells-in-polycaprolactone-scafold-and-using-freeze-thaw-freeze-method
#3
Rana Moradian Tehrani, Hamed Mirzaie, Javad Verdi, Amirhossein Sahebkar, Mahdi Noureddini, Rasoul Salehi, Behrang Alani, Mojtaba Kianmehr
Human adipose tissue has been identified as a viable alternative source for mesenchymal stem cells. SADSCs were isolated from human scalp biopsy and then were characterized by Flow cytometry. SADSCS expressed CD90, CD44, and CD105 but did not express CD45 surface marker. Growth factors were used for chondrogenesis induction. Histology and immunohistology methods and gene expression by real-time PCR 14 days after induced cells have shown the feature of chondrocytes in their morphology and extracellular matrix in both inducing patterns of combination and cycling induction...
January 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29314362/a-small-population-of-resident-limb-bud-mesenchymal-cells-expresses-few-msc-associated-markers-but-the-expression-of-these-markers-is-increased-immediately-after-cell-culture
#4
Jessica Cristina Marín-Llera, Jesús Chimal-Monroy
Skeletal progenitors are derived from resident limb bud mesenchymal cells of the vertebrate embryos. However, it remains poorly understood if they represent stem cells, progenitors or multipotent mesenchymal stromal cells (MSC). Derived-MSC of different adult tissues under in vitro experimental conditions can differentiate into the same cellular lineages that are present in the limb. Here, comparing non-cultured versus cultured mesenchymal limb bud cells, we determined the expression of MSC-associated markers, the in vitro differentiation capacity and their gene expression profile...
January 4, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29313037/soluble-collagen-vi-treatment-enhances-mesenchymal-stem-cells-expansion-for-engineering-cartilage
#5
Piera Smeriglio, Jieun Lee, Nidhi Bhutani
Bone Marrow-derived mesenchymal stem cells (BM-MSC) are an attractive source for cell-based therapies in cartilage injury owing to their efficient differentiation into chondrocytes and their immune-suppressive abilities. However, their clinical use is hampered by a scarcity of cells leading to compromised efficacy. While expansion of human MSC ex vivo can potentially overcome the scarcity of cells, current methods lead to a rapid loss of the stem cell properties. In this study, we report soluble Collagen VI (cartilage pericellular matrix component) as a potential biologic that can expand the MSC population while maintaining the stem cell phenotype as confirmed by expression of the stem cell markers CD105 and CD90...
September 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29307583/transcriptionally-and-functionally-distinct-mesenchymal-subpopulations-are-generated-from-human-pluripotent-stem-cells
#6
Chee Jia Chin, Suwen Li, Mirko Corselli, David Casero, Yuhua Zhu, Chong Bin He, Reef Hardy, Bruno Péault, Gay M Crooks
Various mesenchymal cell types have been identified as critical components of the hematopoietic stem/progenitor cell (HSPC) niche. Although several groups have described the generation of mesenchyme from human pluripotent stem cells (hPSCs), the capacity of such cells to support hematopoiesis has not been reported. Here, we demonstrate that distinct mesenchymal subpopulations co-emerge from mesoderm during hPSC differentiation. Despite co-expression of common mesenchymal markers (CD73, CD105, CD90, and PDGFRβ), a subset of cells defined as CD146hiCD73hi expressed genes associated with the HSPC niche and supported the maintenance of functional HSPCs ex vivo, while CD146loCD73lo cells supported differentiation...
January 3, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29297135/stem-cell-factor-supports-migration-in-canine-mesenchymal-stem-cells
#7
Nathaly Enciso, Luciana L K Ostronoff, Guillermo Mejías, Leticia G León, María Luisa Fermín, Elena Merino, Cristina Fragio, Luis Avedillo, Concepción Tejero
Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC)...
January 3, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29287161/evaluation-of-equine-synovial-derived-extracellular-matrix-scaffolds-seeded-with-equine-synovial-derived-mesenchymal-stem-cells
#8
Nathalie A Reisbig, Hayam A Hussein, Erin Pinnell, Alicia L Bertone
OBJECTIVE To create a bioactive synovium scaffold by infusing decellularized synovial-derived extracellular matrix (synECM) with synovial-derived mesenchymal stem cells (synMSCs). SAMPLE Synovium from the femoropatellar and medial femorotibial joints of equine cadavers. PROCEDURES The synMSCs were cultured in monolayer and not treated or cotransduced to enhance expression of green fluorescent protein (GFP) and human bone morphogenetic protein (BMP)-2. The synECM was decellularized with 0.1% peracetic acid and then seeded with synMSCs (0...
January 2018: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29279046/human-wharton-s-jelly-cells-activate-degenerative-nucleus-pulposus-cells-in-vitro
#9
Zhihua Han, Yan Zhang, Liang Gao, Shujun Jiang, Di-Ke Ruan
In order to investigate the interaction between human Wharton's jelly cells (WJCs) and degenerative nucleus pulposus cells (NPCs), human WJCs were cocultured with degenerative NPCs with or without direct cell-cell contact. WJCs were isolated from the human umbilical cord and degenerative NPCs were isolated from the surgically obtained degenerative intervertebral disc tissue. The isolated WJCs positively expressed CD73, CD105, CD90, CD29, CD166 and human leukocyte antigen (HLA)-ABC, but negatively expressed CD34, CD45, and HLA-DR...
December 27, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29277653/microrna-191-regulated-by-hif-2%C3%AE-is-involved-in-emt-and-acquisition-of-a-stem-cell-like-phenotype-in-arsenite-transformed-human-liver-epithelial-cells
#10
Chao Chen, Qianlei Yang, Dapeng Wang, Fei Luo, Xinlu Liu, Junchao Xue, Ping Yang, Hui Xu, Jiachun Lu, Aihua Zhang, Qizhan Liu
Inorganic arsenic is widely distributed in the environment, and epidemiologic data show a strong association between arsenic exposure and risk of liver cancer. An understanding of the mechanisms underlying development of liver cancer and metastasis would be useful in reducing the incidence and mortality of liver cancer. MicroRNAs (miRs) act as regulators in liver cancer. Here, we show that acute or chronic exposure of human liver epithelial L-02 cells to arsenite increased expression of miR-191. There were decreased levels of BASP-1 and E-cadherin and increased levels of WT-1 and N-cadherin, indicating that arsenite induced epithelial-mesenchymal transition (EMT)...
December 22, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29259721/characterization-of-polydactyly-derived-chondrocyte-sheets-versus-adult-chondrocyte-sheets-for-articular-cartilage-repair
#11
Miki Maehara, Masato Sato, Eriko Toyoda, Takumi Takahashi, Eri Okada, Tomomi Kotoku, Masahiko Watanabe
Background: We previously conducted a first-in-human clinical study of articular cartilage repair using autologous chondrocyte sheets and confirmed the regeneration of hyaline-like cartilage in all eight patients. However, regenerative medicine with autologous chondrocyte sheets requires the harvesting of tissue from healthy regions, and the quality of this tissue varies between individuals. To overcome such limitations, allogeneic transplantation is a promising treatment method, particularly for articular cartilage repair...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29251325/cd90-promotes-cell-migration-viability-and-sphere%C3%A2-forming-ability-of-hepatocellular-carcinoma-cells
#12
Ketao Zhang, Siyao Che, Zheng Su, Shangyou Zheng, Huayao Zhang, Shanglin Yang, Wenda Li, Jianping Liu
Cluster of differentiation (CD)90 (Thy‑1) was proposed as a marker for the liver cancer stem cells that are responsible for tumorigenic activity, however its involvement in the progression of hepatocellular carcinoma (HCC) remains unknown. The aim of the present study was to determine the effect of CD90 on the biological functions of HCC and to investigate the associated circular RNA (circRNA) involved in this process. The analysis of the in vitro data demonstrated that CD90+ cells isolated from SK‑Hep‑1 cells exhibited increased viability, migration and invasive abilities compared with CD90‑ cells...
February 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29245066/immuno-biological-comparison-of-hepatic-stellate-cells-in-a-reverted-and-activated-state
#13
Mehdi Najar, Hussein Fayyad-Kazan, Wissam H Faour, Adil El Taghdouini, Gordana Raicevic, Leo A van Grunsven, Mustapha Najimi, Etienne Sokal, Laurence Lagneaux
Human hepatic stellate cells (HSCs) demonstrated great immunological plasticity with important consequences for liver cell therapy. Activated HSCs (aHSCs) are in vitro reverted (rHSCs) to a quiescent-like phenotype with potential benefit to reduce liver fibrosis. The goal of this study is to establish and compare the immunological profile of activated and in vitro reverted HSCs and to investigate the impact of inflammatory priming on the immunobiology of both HSCs populations. The distribution of inflammatory primed activated and reverted HSCs across the different phases of the cell cycle is assessed by flow cytometry...
December 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29245016/preferential-myofibroblast-differentiation-of-cardiac-mesenchymal-progenitor-cells-in-the-presence-of-atrial-fibrillation
#14
Elisa Gambini, Gianluca Lorenzo Perrucci, Beatrice Bassetti, Gabriella Spaltro, Giulia Campostrini, Maria Chiara Lionetti, Alberto Pilozzi, Federico Martinelli, Andrea Faruggia, Dario DiFrancesco, Andrea Barbuti, Giulio Pompilio
Atrial fibrillation (AF) is characterized by electrical, contractile, and structural remodeling mediated by interstitial fibrosis. It has been shown that human cardiac mesenchymal progenitor cells (CMPCs) can be differentiated into endothelial, smooth muscle, and fibroblast cells. Here, we have investigated, for the first time, the contribution of CMPCs in the fibrotic process occurring in AF. As expected, right auricolae samples displayed significantly higher fibrosis in AF vs control (CTR) patients. In tissue samples of AF patients only, double staining for c-kit and the myofibroblast marker α-smooth muscle actin (α-SMA) was detected...
November 27, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/29242640/effects-of-maternal-obesity-on-wharton-s-jelly-mesenchymal-stromal-cells
#15
Heba Badraiq, Aleksandra Cvoro, Antonio Galleu, Marisa Simon, Cristian Miere, Carl Hobbs, Reiner Schulz, Richard Siow, Francesco Dazzi, Dusko Ilic
We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese (BMI = 19-25; n = 7) and obese (BMI ≥ 30; n = 7) donors undergoing elective Cesarean section, we found that WJ MSC from obese donors showed slower population doubling and a stronger immunosuppressive activity...
December 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29241918/human-amniotic-fluid-stem-cells-have-better-potential-in-early-second-trimester-of-pregnancy-and-can-be-reprogramed-to-ips
#16
S W Steven Shaw, Po-Jen Cheng, Yao-Lung Chang, An-Shine Chao, Tzu-Hao Wang, Shuenn-Dyh Chang, T'sang-T'ang Hsieh, Kuo-Hsuan Chang
OBJECTIVE: To study the difference of amniotic fluid stem cell potential at different gestational age. MATERIALS AND METHODS: Second trimester amniocentesis was performed during 15 to 22nd week of gestational age in a single medical center from 2015 to 2016. Early second trimester amniotic fluid stem cells (E-AFS) and later one (L-AFS) were defined 15-18th week, and 19-22nd week, respectively. Cell characteristics, surface markers and ability to form induced pluripotent stem cells (iPS) were studied...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29241917/pueraria-mirifica-inhibits-17%C3%AE-estradiol-induced-cell-proliferation-of%C3%A2-human-endometrial-mesenchymal-stem-cells
#17
Ta-Chin Lin, Kai-Hung Wang, An-Pei Kao, Kuo-Hsiang Chuang, Tsung-Cheng Kuo
OBJECTIVE: The notion that the human endometrium may contain a population of stem cells has recently been proposed. The mesenchymal stem cells (MSCs) in the endometrium are believed to be responsible for the remarkable regenerative ability of endometrial cells. Estrogens influence the physiological and pathological processes of several hormone-dependent tissues, such as the endometrium. Pueraria mirifica (PM) is a herbal plant that contains several phytoestrogens, including isoflavones, lignans, and coumestans, and is known to exert an estrogenic effect on animal models...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29241405/cell-clusters-are-indicative-of-stem-cell-activity-in-the-degenerate-intervertebral-disc-can-their-properties-be-manipulated-to-improve-intrinsic-repair-of-the-disc
#18
Sharon Brown, Ajay Matta, William Mark Erwin, Sally Roberts, Helen H Gruber, Edward N Hanley, Christopher B Little, James Melrose
The aim of this study was to examine the complexity of the stem cell populations in the intervertebral disc (IVD) and understand their role in disc degeneration with a view to determining whether the resident stem cells could be developed for therapeutic purposes to combat IVD degeneration. Stem cells have been isolated from disc and paradiscal tissues including the notochord, annulus fibrosus (AF), nucleus pulposus (NP), cartilaginous endplate (CEP), ligamentum flavum and vertebral body. Resident AF and NP cells are relatively sparsely distributed occuring as single or occasional doublet cells surrounded by an extensive extracellular matrix (ECM)...
December 14, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29240254/nf-%C3%AE%C2%BAb-regulated-mir-155-via-repression-of-qki-contributes-to-the-acquisition-of-csc-like-phenotype-during-the-neoplastic-transformation-of-hepatic-cells-induced-by-arsenite
#19
Chao Chen, Fei Luo, Qianlei Yang, Dapeng Wang, Ping Yang, Junchao Xue, Xiangyu Dai, Xinlu Liu, Hui Xu, Jiachun Lu, Aihua Zhang, Qizhan Liu
Chronic exposure to arsenite can cause various human tumors. For the initiation and recurrence of human liver cancer, the acquisition of CSC-like properties is essential. In various cancers, microRNAs (miRNAs) act as regulators in induction of CSC-like properties. Liver cancers over-express miR-155, but the mechanism relating miR-155 and arsenite-induced liver cancer is unknown. Here, we show that long-term exposure of L-02 cells to arsenite increases miR-155 levels by activation of NF-κB and leads to the acquisition of CSC-like properties...
December 14, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29239337/globose-basal-cells-for-spinal-cord-regeneration
#20
Durai Murugan Muniswami, Indirani Kanakasabapathy, George Tharion
Spinal cord injury (SCI) is a devastating condition with loss of motor and sensory functions below the injury level. Cell based therapies are experimented in pre-clinical studies around the world. Neural stem cells are located intra-cranially in subventricular zone and hippocampus which are highly invasive sources. The olfactory epithelium is a neurogenic tissue where neurogenesis takes place throughout the adult life by a population of stem/progenitor cells. Easily accessible olfactory neuroepithelial stem/progenitor cells are an attractive cell source for transplantation in SCI...
November 2017: Neural Regeneration Research
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