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https://www.readbyqxmd.com/read/29782555/langerin-dcs-regulate-innate-il-17-production-in-the-oral-mucosa-during-candida-albicans-mediated-infection
#1
Florian Sparber, Tamas Dolowschiak, Sarah Mertens, Laura Lauener, Björn E Clausen, Nicole Joller, Patrizia Stoitzner, Roxane Tussiwand, Salomé LeibundGut-Landmann
The opportunistic fungal pathogen Candida albicans frequently causes diseases such as oropharyngeal candidiasis (OPC) in immunocompromised individuals. Although it is well appreciated that the cytokine IL-17 is crucial for protective immunity against OPC, the cellular source and the regulation of this cytokine during infection are still a matter of debate. Here, we directly visualized IL-17 production in the infected tongue of experimentally infected mice, thereby demonstrating that this key cytokine is expressed by three complementary subsets of CD90+ leukocytes: RAG-dependent αβ and γδ T cells, as well as RAG-independent ILCs...
May 21, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29779281/-effects-of-tumor-necrosis-factor-%C3%AE-on-osteogenic-differentiation-and-notch-signaling-pathway-in-human-periodontal-ligament-stem-cells
#2
Yu Ma, Shu-Hui Li, Xin-Xin Ding, Pei-Ling Wu
OBJECTIVE: To evaluate the effects of tumor necrosis factor-α (TNF-α) on osteogenic differentiation and Notch signaling pathway of periodontal ligament stem cells (PDLSCs) and to investigate the regulatory role of Notch signaling pathway on the osteogenic differentiation of PDLSCs under the influence of TNF-α. METHODS: PDLSCs were obtained through enzyme digestion and tissue block method. The expression levels of stem cell surface markers CD105, CD90, CD146, CD45, and CD31 were detected by fluorescence activated cell sorter (FACS)...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29775846/reproductive-stage-and-sex-steroid-hormone-levels-influence-the-expression-of-mesenchymal-stromal-cell-msc-markers-in-the-equine-endometrium
#3
B Elisabeth Rink, Juliane Kuhl, Cristina L Esteves, Hilari M French, Elaine Watson, Christine Aurich, F Xavier Donadeu
Mesenchymal stem or stromal cells (MSCs) play key roles in tissue homeostasis. In the cyclic equine endometrium, this may be regulated by changes in serum concentrations of sex steroid hormones. This study was designed to investigate the changes in endometrial expression of MSC markers during reproductive cycles in mares and the influence of sex steroid hormones on endometrial MSC proliferation in vitro. Endometrial biopsies were collected from pony mares at different reproductive stages (estrus; day 5 and 13 after ovulation; seasonal anestrus; 20 h and 7days post-partum; n = 5 per stage) and were analyzed by RT-qPCR...
May 4, 2018: Theriogenology
https://www.readbyqxmd.com/read/29774098/establishment-of-primary-cell-culture-and-an-intracranial-xenograft-model-of-pediatric-ependymoma-a-prospect-for-therapy-development-and-understanding-of-tumor-biology
#4
Lorena Favaro Pavon, Tatiana Tais Sibov, Silvia Regina Caminada de Toledo, Daniela Mara de Oliveira, Francisco Romero Cabral, Jean Gabriel de Souza, Pamela Boufleur, Luciana C Marti, Jackeline Moraes Malheiros, Edgar Ferreira da Cruz, Fernando F Paiva, Suzana M F Malheiros, Manoel A de Paiva Neto, Alberto Tannús, Sérgio Mascarenhas de Oliveira, Nasjla Saba Silva, Andrea Maria Cappellano, Antonio Sérgio Petrilli, Ana Marisa Chudzinski-Tavassi, Sérgio Cavalheiro
Background: Ependymoma (EPN), the third most common pediatric brain tumor, is a central nervous system (CNS) malignancy originating from the walls of the ventricular system. Surgical resection followed by radiation therapy has been the primary treatment for most pediatric intracranial EPNs. Despite numerous studies into the prognostic value of histological classification, the extent of surgical resection and adjuvant radiotherapy, there have been relatively few studies into the molecular and cellular biology of EPNs...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29768259/precise-intradermal-injection-of-nanofat-derived-stromal-cells-combined-with-platelet-rich-fibrin-improves-the-efficacy-of-facial-skin-rejuvenation
#5
Zhi-Jie Liang, Xiang Lu, De-Quan Li, Yi-Dan Liang, Dan-Dan Zhu, Fang-Xiao Wu, Xiao-Lin Yi, Ning He, Yan-Qing Huang, Chao Tang, Hong-Mian Li
BACKGROUND/AIMS: The rejuvenation properties of nanofat grafting have been described in recent years. However, it is not clear whether the clinical efficacy of the procedure is attributable to stem cells or linked to other components of adipose tissue. In this study we isolated nanofat-derived stem cells (NFSCs) to observe their biological characteristics and evaluate the efficacy of precise intradermal injection of nanofat combined with platelet-rich fibrin (PRF) in patients undergoing facial rejuvenation treatment...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29765418/isolation-and-characterisation-of-mesenchymal-stem-cells-from-rat-bone-marrow-and-the-endosteal-niche-a-comparative-study
#6
Norhayati Yusop, Paul Battersby, Amr Alraies, Alastair J Sloan, Ryan Moseley, Rachel J Waddington
Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs). This study isolated and characterised heterogeneous MSC populations from each niche and subsequently investigated the effects of extensive cell expansion, analysing population doublings (PDs)/cellular senescence, colony-forming efficiencies (CFEs), MSC cell marker expression, and osteogenic/adipogenic differentiation. CB-MSCs and BM-MSCs demonstrated similar morphologies and PDs, reaching 100 PDs...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29761109/mesenchymal-stem-cells-differentiate-to-endothelial-cells-using-recombinant-vascular-endothelial-growth-factor-a
#7
Mohsen Khaki, Ali Hatef Salmanian, Hamid Abtahi, Ali Ganji, Ghasem Mosayebi
Background: Vascular endothelial growth factor-A (VEGF-A), an endothelial cell-specific mitogen produced by various cell types, plays important roles in cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of mesenchymal stem cells (MSCs) to endothelial cells (ECs). Methods: VEGF-A was expressed in E. coli BL21 (DE3) and BL21 pLysS competent cells with the pET32a expression vector. Recombinant VEGF-A protein expression was verified by SDS-PAGE and western blotting...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29755530/the-effect-of-age-on-the-regenerative-potential-of-human-eyelid-adipose-derived-stem-cells
#8
Mingqi Zhang, Zhuoshi Wang, Yan Zhao, Lirong Zhang, Ling Xu, Liu Cao, Wei He
Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effect of donor age on their characteristics and regenerative potential of HEASCs in vitro. The HEASCs were isolated from patients of three groups: (1) <20 years ( n = 4), (2) >20 years, <45 years ( n = 5), and (3) >55 years ( n = 4)...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29741994/development-of-a-novel-explant-culture-method-for-the-isolation-of-mesenchymal-stem-cells-from-human-breast-tumor
#9
Koushan Sineh Sepehr, Alireza Razavi, Mohsen Saeidi, Majid Mossahebi-Mohammadi, Meghdad Abdollahpour-Alitappeh, Seyed Mahmoud Hashemi
BACKGROUND: Mesenchymal stem cells (MSCs) were isolated from various sources, including various types of tumors. However choosing an appropriate isolation method is an important step in obtaining cells with optimal quality and yield in companion with economical considerations. The purpose of this study was to isolate of more pure MSCs from human breast tumor tissue by a modified explant culture method. METHODS AND MATERIALS: The tumor tissues (n = 8) was cut into 1 to 3-mm cube-like pieces (explant)...
May 9, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29731830/rho-rho-associated-protein-kinase-signaling-pathway-mediated-downregulation-of-runt-related-transcription-factor-2-expression-promotes-the-differentiation-of-dental-pulp-stem-cells-into-odontoblasts
#10
Xiaoqing Huang, Xiaoling Chen, Hongbai Chen, Dongwei Xu, Chen Lin, Bin Peng
The present study investigated the role of runt-related transcription factor 2 (Runx2) in regulating the differentiation of human dental pulp stem cells (hDPSCs) into odontoblasts under the mediation of the Rho/Rho-associated protein kinase (ROCK) signaling pathway. hDPSCs and human bone marrow mesenchymal stem cells (hBMSCs) were mineralized to induce differentiation. The expression levels of odontoblast- and osteoblast-specific proteins, dentin sialophosphoprotein (DSPP), osteocalcin (OCN) and Runx2, were measured using western blot analysis...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29722127/the-shh-gli-axis-regulates-cd90-mediated-liver-cancer-stem-cell-function-by-activating-the-il6-jak2-pathway
#11
Ketao Zhang, Siyao Che, Chuzhi Pan, Zheng Su, Shangyou Zheng, Shanglin Yang, Huayao Zhang, Wenda Li, Weidong Wang, Jianping Liu
The cell surface antigen CD90 has recently been established as a promising marker for liver cancer stem cells. This study aimed to investigate potential implications of SHH/Gli signalling in CD90+ liver cancer stem cells. Correlation of the expression of SHH signalling components and CD90 in liver cancer cells and clinical tissues, as well as in enriched CD90+ liver cancer stem cells and the TCGA database, were analysed by quantitative RT-PCR, Western blotting and flow cytometry. Functional analysis was conducted by siRNA-mediated CD90, Gli1 and Gli3 gene knockdown, SHH treatment and application of the JAK2 inhibitor AZD1480 and IL6 neutralizing antibody in CD90+ liver cancer stem cells, followed by cell proliferation, migration, sphere formation and tumorigenicity assays...
May 2, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29714147/epithelial-in-vitro-differentiation-of-mesenchymal-stem-cells
#12
Alvaro Sierra-Sanchez, Alexandra Ordonez-Luque, Olga Espinosa-Ibanez, Antonio Ruiz-Garcia, Salvador Arias-Santiago
Mesenchymal stem cells or mesenchymal stromal cells (MSCs) are non-hematopoietic stromal cells that reside in and have been isolated from a variety of adult or fetal tissues such as adipose tissue, bone marrow and umbilical cord Wharton's jelly, among others. Because they are a heterogeneous population, International Society for Cellular Therapy has established 3 minimum criteria to characterize MSCs in vitro: i) adherence to plastic, ii) differentiation potential (osteogenic, chondrogenic and adipogenic lineages) and iii) expression of specific surface antigens (CD73+, CD90+, CD105+, CD34-, CD45-, CD11b-, CD14-, CD19-, CD79a-, HLA-DR-)...
May 1, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29705293/cytotoxicity-of-radiocontrast-dyes-in-human-umbilical-cord-mesenchymal-stem-cells
#13
Christina McKee, Naimisha Beeravolu, Shreeya Bakshi, Bryan Thibodeau, George Wilson, Mick Perez-Cruet, G Rasul Chaudhry
Radiocontrast dyes are used for a wide range of diagnostic procedures for enhancing the image of anatomical structures, pain targets, and vascular uptake. While some of these dyes show toxicity to primary cells, their effect on stem cells, particularly mesenchymal stem cells (MSCs), is unknown. This study investigates the cytotoxic effects of two clinically used radiocontrast dyes, iohexol and iopamidol, on bone marrow and human umbilical cord MSCs. Exposure to these dyes significantly affected morphology of MSCs from both sources, as treated cells appeared transparent and no longer fibroblastoid...
April 26, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29699510/putative-cancer-stem-cells-may-be-the-key-target-to-inhibit-cancer-cell-repopulation-between-the-intervals-of-chemoradiation-in-murine-mesothelioma
#14
Licun Wu, Walter Blum, Chang-Qi Zhu, Zhihong Yun, Laszlo Pecze, Mikihiro Kohno, Mei-Lin Chan, Yidan Zhao, Emanuela Felley-Bosco, Beat Schwaller, Marc de Perrot
BACKGROUND: Cancer cell repopulation during chemotherapy or radiotherapy is a major factor limiting the efficacy of treatment. Cancer stem cells (CSC) may play critical roles during this process. We aim to demonstrate the role of mesothelioma stem cells (MSC) in treatment failure and eventually to design specific target therapies against MSC to improve the efficacy of treatment in malignant mesothelioma. METHODS: Murine mesothelioma AB12 and RN5 cells were used to compare tumorigenicity in mice...
April 27, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29688163/human-amniotic-fluid-stem-cells-hafscs-expressing-p21-and-cyclin-d1-genes-retain-excellent-viability-after-freezing-with-dimethyl-sulfoxide-dmso
#15
Shiva Gholizadeh-Ghaleh Aziz, Zahra Fardyazar, Fatima Pashaei-Asl, Mohammad Rahmati-Yamchi, Khodadad Khodadadi, Maryam Pashaiasl
Human amniotic fluid stem cells (hAFSCs) have features intermediate between embryonic and adult SCs, can differentiate into lineages of all three germ layers, and do not develop into tumors in vivo. Moreover, hAFSCs can be easily obtained in routine procedures and there is no ethical or legal limitations regarding their use for clinical and experimental applications. The aim of this study was to assess the effect of slow freezing/thawing and two different concentrations of DMSO (10% DMSO + 90% fetal bovine serum [FBS] and 5% DMSO + 95% FBS) on the survival of hAFSCs...
April 24, 2018: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29687448/stem-cell-like-populations-and-immunoregulatory-molecules-in-periodontal-granulation-tissue
#16
D A Apatzidou, C Nile, A Bakopoulou, A Konstantinidis, D F Lappin
BACKGROUND AND OBJECTIVES: Determine the presence of mesenchymal stem cells (MSCs) in healthy periodontal tissue and periodontal granulation tissue (GT) and explore associations between immuno-regulatory molecules and selected subgingival microorganisms. MATERIAL AND METHODS: Mesenchymal stem cells were isolated, propagated and characterised by flow cytometry from a region of healthy gingival tissue and inflamed GT of 10 systemically healthy non-smokers with chronic periodontitis...
April 23, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29682351/comparative-analysis-of-cellular-and-growth-factor-composition-in-bone-marrow-aspirate-concentrate-and-platelet-rich-plasma
#17
Hisashi Sugaya, Tomokazu Yoshioka, Toshiki Kato, Yu Taniguchi, Hiroshi Kumagai, Kojiro Hyodo, Osamu Ohneda, Masashi Yamazaki, Hajime Mishima
The purpose of this study was to quantify the stem cell and growth factor (GF) contents in the bone marrow aspirate concentrate (BMAC) and platelet-rich plasma (PRP) prepared from whole blood using a protocol established in our laboratory. We examined 10 patients with osteonecrosis of the femoral head who were treated by autologous BMAC transplantation at our hospital between January 2015 and June 2015. We quantified CD34+ and CD31-CD45-CD90+CD105+ cells in BMAC and PRP by flow cytometry. Additionally, we measured various GFs, that is, basic fibroblast growth factor (b-FGF), platelet-derived growth factor-BB (PDGF-BB), vascular endothelial growth factor (VEGF), transforming growth factor- β 1 (TGF- β 1), and bone morphogenetic protein-2 (BMP-2) in BMAC and PRP using enzyme-linked immunosorbent assays and statistical analyses...
2018: Bone Marrow Research
https://www.readbyqxmd.com/read/29679460/limbal-niche-cells-are-a-potent-resource-of-adult-mesenchymal-progenitors
#18
REVIEW
Ping Guo, Hong Sun, Yuan Zhang, Sean Tighe, Shuangling Chen, Chen-Wei Su, Yongsong Liu, Hongxia Zhao, Min Hu, Yingting Zhu
Limbal niche cells located in the limbal Palisades of Vogt are mesenchymal stem cells that reside next to limbal basal epithelial cells. Limbal niche cells are progenitors that express embryonic stem cell markers such as Nanog, Nestin, Oct4, Rex1, Sox2 and SSEA4, mesenchymal cell markers such as CD73, CD90 and CD105, and angiogenesis markers such as Flk-1, CD31, CD34, VWF, PDGFRβ and α-SMA, but negative for CD45. In addition, the stemness of limbal niche cells can be maintained during their cell culture in a three-dimension environment...
April 20, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29675567/human-urine-derived-stem-cells-play-a-novel-role-in-the-treatment-of-stz-induced-diabetic-mice
#19
Tianxue Zhao, Deng Luo, Yun Sun, Xin Niu, Yang Wang, Chen Wang, Weiping Jia
Human urine-derived stem cells (hUSCs) are a potential stem cell source for cell therapy. However, the effect of hUSCs on glucose metabolism regulation in type 1 diabetes was not clear. Therefore, the aim of the study was to evaluate whether hUSCs have protective effect on streptozotocin (STZ)-induced diabetes. hUSCs were extracted and cultivated with a special culture medium. Flow cytometry analysis was applied to detect cell surface markers. BALB/c male nude mice were either injected with high-dose STZ (HD-STZ) or multiple low-dose STZ (MLD-STZ)...
April 19, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29675371/mesenchymal-stem-cells-derived-exosomes-ameliorate-blue-light-stimulation-in-retinal-pigment-epithelium-cells-and-retinal-laser-injury-by-vegf-dependent-mechanism
#20
Guang-Hui He, Wei Zhang, Ying-Xue Ma, Jing Yang, Li Chen, Jian Song, Song Chen
AIM: To observe the effect of exosomes derived from human umbilical cord blood mesenchymal stem cells (hUCMSCs) on the expression of vascular endothelial growth factor-A (VEGF-A) in blue light injured human retinal pigment epithelial (RPE) cells and laser-induced choroidal neovascularization (CNV) in rats. METHODS: Exosomes were isolated from hUCMSCs and characterized by transmission electron microscope and Western blot. MSCs-derived exosomes were cultured with RPE cells exposed to blue light...
2018: International Journal of Ophthalmology
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