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https://www.readbyqxmd.com/read/28212265/variations-in-oxidative-stress-levels-in-3-days-follow-up-in-ultramarathon-mountain-race-athletes
#1
Ypatios Spanidis, Dimitrios Stagos, Marina Orfanou, Nikolaos Goutzourelas, David Bar-Or, Demetrios Spandidos, Demetrios Kouretas
Spanidis, Y, Stagos, D, Orfanou, M, Goutzourelas, N, Bar-or, D, Spandidos, D, and Kouretas, D. Variations in oxidative stress levels in 3 days follow-up in ultramarathon mountain race athletes. J Strength Cond Res 31(3): 582-594, 2017-The aim of the present study was the monitoring of the redox status of runners participating in a mountain ultramarathon race of 103 km. Blood samples from 12 runners were collected prerace and 24, 48, and 72 hours postrace. The samples were analyzed by using conventional oxidative stress markers, such as protein carbonyls (CARB), thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC) in plasma, as well as glutathione (GSH) levels and catalase (CAT) activity in erythrocytes...
March 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28150507/the-microinr-portable-coagulometer-analytical-quality-and-user-friendliness-of-a-pt-inr-point-of-care-instrument
#2
Pia Bükmann Larsen, Elin Storjord, Åsne Bakke, Tone Bukve, Mikael Christensen, Joakim Eikeland, Vegar Engeland Haugen, Kristin Husby, Rie McGrail, Solveig Meier Mikaelsen, Grete Monsen, Mette Fogh Møller, Jan Nybo, Jesper Revsholm, Aslaug Johanne Risøy, Unni Marie Skålsvik, Heidi Strand, Reyes Serrano Teruel, Elvar Theodorsson
Regular measurement of prothrombin time as an international normalized ratio PT (INR) is mandatory for optimal and safe use of warfarin. Scandinavian evaluation of laboratory equipment for primary health care (SKUP) evaluated the microINR portable coagulometer (microINR(®)) (iLine Microsystems S.L., Spain) for measurement of PT (INR). Analytical quality and user-friendliness were evaluated under optimal conditions at an accredited hospital laboratory and at two primary health care centres (PHCCs). Patients were recruited at the outpatient clinic of the Laboratory of Medical Biochemistry, St Olav's University Hospital, Trondheim, Norway (n = 98) and from two PHCCs (n = 88)...
April 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28133956/development-of-a-novel-automated-screening-method-for-detection-of-fviii-inhibitors
#3
M S Evans, K J Donaldson, M E Eyster
INTRODUCTION: Factor VIII activity is routinely determined by measuring the activated partial thromboplastin time (aPTT) of a patient plasma sample and determining percent activity from a standard curve. To maximize the detection of a clotting factor inhibitor, a subjective assessment of parallelism of a patient curve compared with a standard curve is performed. We developed and validated an automated objective method to assess parallelism as a rapid screening tool for detection of an inhibitor to factor VIII during routine FVIII assays...
January 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28130089/novel-feed-including-bioactive-compounds-from-winery-wastes-improved-broilers-redox-status-in-blood-and-tissues-of-vital-organs
#4
Sotiria Makri, Ioannis Kafantaris, Dimitrios Stagos, Theodora Chamokeridou, Konstantinos Petrotos, Konstantinos Gerasopoulos, Anastasios Mpesios, Nikolaos Goutzourelas, Stylianos Kokkas, Panagiotis Goulas, Dimitrios Komiotis, Dimitrios Kouretas
Currently, there is a great interest in the production of animal feed with antioxidant activity. The aim of this study was to examine the potential antioxidant effects of a feed supplemented with grape pomace (GP), a winery by-product with high environmental load, in chickens. Broilers of 15 days post birth were separated into two groups fed either with standard diet or with diet supplemented with GP for 35 days. Blood and tissues collections were performed after feeding for 15 and 35 days with the experimental diet (i...
January 24, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27848925/analytical-performances-of-hemoclot-protein-c-reagent-on-acl-top-analyzer
#5
COMPARATIVE STUDY
Leyla Calmette, Nicole Charpentier, Caroline Tircot, Delphine Bigot, Claire Dunois, Jean Amiral, Marcelle Tetegan, Sonnthida Sep Hieng, Jean-Yves Peltier
Our study aimed to evaluate and validate according to standard NF EN ISO 15189 the original protocol ajustement of Hemoclot Protein C (PC) (Hyphen BioMed), clotting-based assay of PC on ACL TOP analyzer (Werfen/Instrumentation Laboratory). We evaluated the performance in terms of imprecision and we validate additional parameters in range B required by the SH GTA 04 (COFRAC): repeatability, reproducibility, detection and quantification limits, limits of linearity, stability, inter-samples and inter-reagents contamination, inaccuracy, evaluation of interferences (hemolysis, bilirubinemia and chyles)...
December 1, 2016: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/27780603/sensitivity-of-routine-coagulation-assays-to-direct-oral-anticoagulants-patient-samples-versus-commercial-drug-specific-calibrators
#6
Ming Sheng Lim, Kent Chapman, Priscilla Swanepoel, Anoop K Enjeti
Most studies on the sensitivities of coagulation assays to direct oral anticoagulants (DOACs) are based on normal plasma spiked with anticoagulant in the laboratory. Recent studies have shown that reagent sensitivity varies significantly depending on whether spiked or patient samples are used. The aim of this study was to compare the sensitivities of routine coagulation assays in patient samples and commercial drug specific calibrators using commonly used activated partial thromboplastin time (APTT) and prothrombin time (PT) reagents (i...
December 2016: Pathology
https://www.readbyqxmd.com/read/27753147/grape-pomace-improves-antioxidant-capacity-and-faecal-microflora-of-lambs
#7
I Kafantaris, B Kotsampasi, V Christodoulou, E Kokka, P Kouka, Z Terzopoulou, K Gerasopoulos, D Stagos, C Mitsagga, I Giavasis, S Makri, K Petrotos, D Kouretas
Grape pomace is a by-product of winemaking process and rich in bioactive compounds such as plant polyphenols having antioxidant and antimicrobial activities. As known, oxidative stress may cause a number of pathological conditions in farm animals and thus affecting animal welfare and production. Moreover, pathogenic bacteria affect animals' health status. Thus, the objective of this study was to investigate whether lambs' feed supplemented with grape pomace enhances the antioxidant mechanisms and reduces the growth of pathogenic bacteria...
October 18, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27725023/correlation-between-rivaroxaban-xarelto-plasma-activity-patient-clinical-variables-and-outcomes-in-a-south-african-centre
#8
S Louw, N P Saragas, P N Ferrao, T F Chirwa, B F Jacobson
BACKGROUND: Low-molecular-weight heparin and vitamin K antagonists such as warfarin are the gold standard for prohylaxis and treatment of venous thromboembolic disease (VTED). Direct oral anticoagulants (DOACs) result in predictable anticoagulation with significantly reduced inter- and intra-patient variability. DOAC absorption is rapid, with a short half-life and relatively few drug interactions. DOACs are effective and safe at fixed doses without activity monitoring. However, specific situations may require assessment of accurate drug activity...
September 8, 2016: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/27589706/development-and-validation-of-a-kit-to-measure-drink-antioxidant-capacity-using-a-novel-colorimeter
#9
Alexandros Priftis, Dimitrios Stagos, Nikolaos Tzioumakis, Konstantinos Konstantinopoulos, Anastasia Patouna, Georgios E Papadopoulos, Aristides Tsatsakis, Dimitrios Kouretas
Measuring the antioxidant capacity of foods is essential, as a means of quality control to ensure that the final product reaching the consumer will be of high standards. Despite the already existing assays with which the antioxidant activity is estimated, new, faster and low cost methods are always sought. Therefore, we have developed a novel colorimeter and combined it with a slightly modified DPPH assay, thus creating a kit that can assess the antioxidant capacity of liquids (e.g., different types of coffee, beer, wine, juices) in a quite fast and low cost manner...
August 30, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27554596/increase-in-antioxidant-activity-by-sheep-goat-whey-protein-through-nuclear-factor-like-2-nrf2-is-cell-type-dependent
#10
Efthalia Kerasioti, Dimitrios Stagos, Aggeliki Tzimi, Dimitrios Kouretas
The aim of the present study was to investigate the molecular mechanisms through which sheep/goat whey protein exerts its antioxidant activity. Thus, it was examined whey protein's effects on the expression of transcription factor, nuclear factor-like 2 (Nrf2) and on the expression and activity of a number of antioxidant and phase II enzymes, superoxide dismutase (SOD), catalase (CAT), heme oxygenase 1 (HO-1), synthase glutamyl cysteine (GCS) and glutathione-s-transferase (GST), in muscle C2C12 and EA.hy926 endothelial cells...
November 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27465629/variations-in-oxidative-stress-levels-in-three-days-follow-up-in-ultra-marathon-mountain-race-athletes
#11
Spanidis Ypatios, Stagos Dimitrios, Orfanou Marina, Goutzourelas Nikolaos, Bar-Or David, Spandidos Demetrios, Kouretas Demetrios
The aim of the present study was the monitoring of the redox status of runners participating in a mountain ultra-marathon race of 103 km. Blood samples from 12 runners were collected pre race and 24, 48 and 72 h post race. The samples were analyzed by using conventional oxidative stress markers, such as protein carbonyls (CARB), thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC) in plasma, as well as glutathione (GSH) levels and catalase (CAT) activity in erythrocytes. In addition, two novel markers the static oxidation-reduction potential marker (sORP) and the capacity oxidation-reduction potential (cORP) were measured in plasma...
July 25, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27331311/dilute-russell-s-viper-venom-time-reagents-in-lupus-anticoagulant-testing-a-well-considered-choice
#12
Barbara Depreter, Katrien M J Devreese
BACKGROUND: Lupus anticoagulant (LAC) detection represents diagnostic challenges among which the multitude of available reagents and interference by anticoagulant treatment. One of the two advised tests is the dilute Russell's viper venom time (dRVVT). However, it is currently not clear whether all dRVVT reagents may be considered equivalent. The objective of the study was to evaluate the diagnostic performance of two dRVVT reagents, with special attention to the influence of anticoagulant therapy...
January 1, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27306848/effects-of-haemolysis-icterus-and-lipaemia-on-coagulation-tests-as-performed-on-stago-sta-compact-max-analyser
#13
A Woolley, J-L Golmard, S Kitchen
INTRODUCTION: Haemolysis, icterus and lipaemia (HIL) may affect haemostasis test results. This may be influenced by the level of interfering substance and the reagents and end-point detection system used. METHODS: We assessed the influence of HIL on prothrombin time, activated partial thromboplastin time and fibrinogen assay using a viscosity-based detection analyser. RESULTS: Spontaneous haemolysis that occurred during sample collection and processing had no effect on PT with either a rabbit tissue factor extract or recombinant human tissue factor reagents...
August 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27237807/biological-diagnosis-of-von-willebrand-disease-analytical-characteristics-of-innovance-vwf-ac-assay-kit-on-sta-r-evolution-expert-series-analyzer-stago
#14
Cécile Florin, Olivier Garraud, Serge Molliex, Brigitte Tardy, Lydia Campos, Carine Scherrer
The Innovance VWF:Ac test (Siemens) has the particularity to assess the binding capacity of von Willebrand factor (VWF) to recombinant platelet GPIb mutated in the absence of ristocetin. Our study aimed to evaluate and validate according to standard NF EN ISO 15189 the original protocol adaptation on STA-R Evolution series analyser (Diagnostica Stago). We evaluated the performance in terms of imprecision and we validate additional parameters necessary in range B as recommended by the SH GTA 04 (Cofrac). We compared the new assay with the reference assay: ristocetin cofactor activity (VWF:RCo) performed on the BCS-XP analyser by testing retrospectively samples from 82 healthy normal subjects and 61 patients with von Willebrand disease (VWD)...
June 1, 2016: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/27127549/antioxidant-effects-of-sheep-whey-protein-on-endothelial-cells
#15
Efthalia Kerasioti, Dimitrios Stagos, Vasiliki Georgatzi, Erinda Bregou, Alexandros Priftis, Ioannis Kafantaris, Dimitrios Kouretas
Excessive production of reactive oxygen species (ROS) may cause endothelial dysfunction and consequently vascular disease. In the present study, the possible protective effects of sheep whey protein (SWP) from tert-butyl hydroperoxide- (tBHP-) induced oxidative stress in endothelial cells (EA.hy926) were assessed using oxidative stress biomarkers. These oxidative stress biomarkers were glutathione (GSH) and ROS levels determined by flow cytometry. Moreover, thiobarbituric acid-reactive substances (TBARS), protein carbonyls (CARB), and oxidized glutathione (GSSG) were determined spectrophotometrically...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27107088/assessment-of-fatty-acid-allocation-in-plasma-and-tissues-in-piglets-using-feed-supplemented-with-byproducts-from-processed-olive-mill-wastewater
#16
Konstantinos Gerasopoulos, Dimitrios Stagos, Alexandros Krouezas, Christina Karaveli, Christina Barda, Helen Gkika, Dimitrios Mitsiou, Konstantinos Petrotos, Panagiotis Goulas, Demetrios Kouretas
BACKGROUND/AIM: A previous study revealed the improvement of redox status in blood and tissues of young piglets (ablactation period), that consumed feed containing polyphenolic additives from byproducts of processed olive mill wastewater (OMWW). The polyphenolic additives strengthened the antioxidant defense of the piglets. Herein we analyzed the fatty acid (FA) composition of these animals in various tissues. MATERIALS AND METHODS: The steps followed during the analysis were: Preparation and isolation of byproducts containing polyphenolic compounds from OMWW processing, silage and piglet feed preparation, blood and tissue collection, fatty acid methyl esters synthesis and GC/MS analysis...
May 2016: In Vivo
https://www.readbyqxmd.com/read/26998009/assessment-of-the-redox-status-in-patients-with-metabolic-syndrome-and-type-2-diabetes-reveals-great-variations
#17
Ypatios Spanidis, Anastasios Mpesios, Dimitrios Stagos, Nikolaos Goutzourelas, David Bar-Or, Maria Karapetsa, Epaminondas Zakynthinos, Demetrios A Spandidos, Aristides M Tsatsakis, George Leon, Demetrios Kouretas
The aim of the present study was to examine the effectiveness of a new redox status marker, the static oxidation reduction potential (sORP), for assessing oxidative stress in 75 patients with metabolic syndrome (MetS) and type 2 diabetes (T2D). A total of 35 normal subjects were used as the controls. Moreover, conventional markers of oxidative stress were assessed, such as thiobarbituric acid reactive substances (TBARS), protein carbonyls, the total antioxidant capacity in plasma, glutathione (GSH) levels and catalase (CAT) activity in erythrocytes...
March 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/26921123/polyvinylpyrrolidone-induced-artefactual-prolongation-of-activated-partial-thromboplastin-times-in-intravenous-drug-users-with-renal-failure
#18
A H Kristoffersen, T K Bjånes, S Jordal, S Leh, F Leh, E Svarstad
Unexpectedly prolonged activated partial thromboplastin times (APTTs) were noted in several patients with chronic renal insufficiency and a history of intravenous drug abuse. Deposits of polyvinylpyrrolidone (PVP), an excipient in one oral methadone solution used in opioid maintenance programs, were found in renal biopsies. One case is described in detail, followed by a summary of findings in 11 other patients and results of an in vitro experiment where plasma was spiked with PVP at increasing concentrations...
February 26, 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/26915943/validation-of-the-coaguchek-xs-international-normalised-ratio-point-of-care-analyser-in-patients-at-charlotte-maxeke-johannesburg-academic-hospital-south-africa
#19
E L Benade, B F Jacobson, S Louw, E Schapkaitz
BACKGROUND: Measurement of the international normalised ratio (INR) is essential in the management of patients on long-term warfarin therapy. The CoaguChek XS portable coagulometer is a point-of-care test for INR measurement. It offers the advantage of improved patient accessibility, particularly in peripheral clinics. OBJECTIVES: To evaluate the clinical utility of the CoaguChek XS for monitoring of patients on standard warfarin therapy (INR 2 - 3) as well as those with mechanical heart valve replacements (INR 2...
March 2016: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/26889231/variations-in-oxidative-stress-markers-in-elite-basketball-players-at-the-beginning-and-end-of-a-season
#20
Ypatios Spanidis, Nikolaos Goutzourelas, Dimitrios Stagos, Anastasios Mpesios, Alexandros Priftis, David Bar-Or, Demetrios A Spandidos, Aristides M Tsatsakis, George Leon, Demetrios Kouretas
The aim of the present study was to examine the changes occuring in the redox status in male basketball players at the beginning and end of a highly competitive season. For this purpose, the redox status of 14 professional athletes of a European basketball club was examined at 2 different time points, at the beginning (phase 1) and at the end of the season (phase 2). The redox status was assessed in blood using conventional oxidative stress markers, such as thiobarbituric acid reactive substances (TBARS), protein carbonyls (CARB) and the total antioxidant capacity (TAC) in plasma, as well as glutathione (GSH) levels and catalase (CAT) activity in erythrocytes...
January 2016: Experimental and Therapeutic Medicine
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