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G W Kershaw, K Dissanayake, V M Chen, T-L Khoo
INTRODUCTION: Chromogenic substrate assays (CSA) to measure Factor IX (FIX) have recently become commercially available. However, information on their performance characteristics and use in diagnostic haemostasis laboratories remains limited. AIM: To evaluate the Hyphen Biomed (Hyphen) and Rossix FIX CSAs on fully automated coagulation analysers and compare them to the FIX one-stage assay (OSA). This study was conducted in a tertiary referral haemostasis laboratory associated with a haemophilia treatment centre...
February 28, 2018: Haemophilia: the Official Journal of the World Federation of Hemophilia
Mesude Falay, Mehmet Senes, Dogan Yücel, Turan Turhan, Simten Dagdaş, Melike Pekin, Namik K Nazaroglu, Gülsüm Özet
BACKGROUND: This study is a retrospective evaluation of patients who were subject to mixing study in our laboratory due to prolonged APTT. The preliminary diagnoses, clinical manifestations, and results of additional ordered tests were reviewed. The study aims to investigate whether repeating APTT test with a different assay prior to performing mixed study in patients with prolonged APTT would be a better alternative algorithmic approach in order to save both time and costs. METHODS: We retrospectively evaluated 166 patients (65 females and 101 males) who were subject to mixing study due to isolated prolonged APTT...
February 27, 2018: Journal of Clinical Laboratory Analysis
Efthalia Kerasioti, Dimitrios Stagos, Aristides M Tsatsakis, Demetrios A Spandidos, Ioannis Taitzoglou, Demetrios Kouretas
The purpose of the present study is to estimate the effects of sheep/goat whey protein dietary supplementation on the redox status of blood and tissues of rats. Twelve male Wistar rats were divided into the control group (standard commercial diet) and whey group [standard commercial diet + sheep/goat whey protein (1 g kg b.w/day)] (6 rats/group). The animals were maintainted on their respective diet for 28 days. At the end of the experimental period, reduced glutathione, catalase activity, total antioxidant capacity, thiobarbituric reactive substances, protein carbonyls and the decomposition rate of H2O2 were measured in blood and tissues of rats...
February 20, 2018: Molecular Medicine Reports
Sotiria Makri, Ioannis Kafantaris, Salomi Savva, Polyxeni Ntanou, Dimitrios Stagos, Ioannis Argyroulis, Basiliki Kotsampasi, Vladimiros Christodoulou, Konstantinos Gerasopoulos, Konstantinos Petrotos, Dimitrios Komiotis, Demetrios Kouretas
BACKGROUND/AIM: The aim of the present study was to investigate the antioxidant effects of a feed supplemented with polyphenolic additives from olive mill wastewater (OMW) on lambs. MATERIALS AND METHODS: Lambs received breast milk until the postnatal period, and then they were divided into two groups and received control and OMW feed for 55 days. Redox biomarkers were measured in blood and tissues at days 15, 42 and 70 after feeding. RESULTS: Feed supplemented with OMW reduced thiobarbituric acid reactive species and protein carbonyls and increased total antioxidant capacity, glutathione and catalase activity in both blood and tissues...
March 2018: In Vivo
R Marlu, T Jouve, B Polack, P Sié, V Mémier
We read with great interest the recent article by Jaffer et al. (1) comparing the ecarin chromogenic assay (ECA) and the diluted thrombin time (dTT) with high-performance liquid chromatography mass spectrometry assay (HPLC/MS) for the quantification of dabigatran concentrations in plasma. In this article, the ECA was performed using a STA-ECA-II kit with STA dabigatran calibrators (Diagnostica Stago, Asnières sur Seine, France) and dTT was performed using the Hemoclot Thrombin Inhibitor assay (HTI) (Hyphen BioMed, Neuville-sur-Oise, France)...
February 20, 2018: Journal of Thrombosis and Haemostasis: JTH
Alexandros Priftis, Eleftheria-Maria Panagiotou, Konstantinos Lakis, Christina Plika, Maria Halabalaki, Georgia Ntasi, Aristidis S Veskoukis, Dimitrios Stagos, Leandros A Skaltsounis, Dimitrios Kouretas
Coffee is one of the most highly consumed beverages with potential beneficial health implications, however its molecular mechanism of action has not been completely elucidated yet. To that cause, the polyphenolic composition of different coffee extracts (from Light, Medium and Dark roasts as well as green beans) was examined by UHPLC-HRMS analysis, indicating chlorogenic acids isomers as the main constituents. In the following step, the toxicity of the extracts was tested in myoblasts and endothelial cells and differential toxicity of green and roasted samples was displayed as the myoblasts were more sensitive to green coffee extracts, in contrast to the endothelial cells...
February 13, 2018: Food and Chemical Toxicology
A Silveira, A Carlo, M Adam, O McLeod, P Lundman, S Boquist, B J Woodhams, A Hamsten
INTRODUCTION: Factor VII activation occurs postprandially. A proportion of activated factor VII (VIIa) circulates in complex with antithrombin (VIIaAT). Our primary objective was to assess the effects of postprandial lipemia on circulating VIIaAT, procoagulant phospholipid (PPL) activity, and thrombin generation. METHODS: Plasma samples from postmyocardial infarction patients (n = 40) and controls (n = 39) were taken before and at 3 and 6 hours during a standardized oral fat tolerance test (OFTT)...
January 22, 2018: International Journal of Laboratory Hematology
M Cini, C Legnani, B Cosmi, S Testa, C Dellanoce, O Paoletti, R Marcucci, D Poli, R Paniccia, V Pengo, A Tripodi, G Palareti
INTRODUCTION: Several specific assays are commercially available to determine dabigatran anticoagulant activity. Aims of this multicenter and multiplatform study were to compare five methods for dabigatran measurement and investigate their performances in the low concentration range. METHODS: Dabigatran levels were analyzed in 295 plasma samples from patients enrolled in the START-Laboratory Register by the following methods using dedicated calibrators and controls: STA-ECA II (Diagnostica Stago), standard and low range Hemoclot Thrombin Inhibitors (Hyphen BioMed), Direct Thrombin Inhibitor Assay (Instrumentation Laboratory), Direct Thrombin Inhibitor Assay (Siemens), Technoclot DTI (Technoclone)...
January 3, 2018: International Journal of Laboratory Hematology
Wendy S Baker, Kathleen J Albright, Heidi Spratt, Megan Berman, Peggy A Mann, Jaime Unabia, John R Petersen
The data presented here was produced as part of an evaluation of the performance of the CoaguChek XS point-of-care coagulation analyzer, which is discussed in the research article "POCT PT INR - Is it adequate for Patient Care? A Comparison of the Roche Coaguchek XS vs. Stago Star vs. Siemens BCS in Patients Routinely Seen in an Anticoagulation Clinic" (Baker et al., in press) [1]. An effort to reconcile discrepancies in the patient INR result distributions from different central lab instruments (Stago Star and Siemens BCS) with the PT/INR line method is described (Poller et al...
February 2018: Data in Brief
Alexandros Priftis, Nikolaos Goutzourelas, Maria Halabalaki, Georgia Ntasi, Dimitrios Stagos, Grigorios D Amoutzias, Leandros A Skaltsounis, Dimitrios Kouretas
Coffee and grape contain various bioactive compounds like polyphenols that may exert beneficial effects, especially antioxidant activity, on human health upon consumption. However, the molecular mechanisms through which these effects are achieved are not fully elucidated. Thus, in the present study in order to investigate these mechanisms, a whole genome expression DNA microarray analysis was carried out in myoblasts treated with polyphenols of coffee and grape pomace at concentrations that improved the redox status...
November 23, 2017: Mechanisms of Ageing and Development
F Stufano, L Baronciani, D Mane-Padros, G Cozzi, S Faraudo, F Peyvandi
INTRODUCTION: Laboratory diagnosis of von Willebrand disease (VWD) is made by the measurement of von Willebrand factor (VWF) protein level and its activities. Current VWF activity tests include ristocetin cofactor and collagen binding (VWF:CB) assays. AIM: We have undertaken an evaluation of a new fully automated VWF:CB assay relative to an established enzyme-linked immunosorbent assay (ELISA) method. METHODS: The two analytical systems operate with different detection principles: a chemiluminescent method performed on ACL AcuStar Analyzer (the former) and a colorimetric ELISA by Asserachrom Stago (the latter) (type III collagen from human placenta)...
January 2018: Haemophilia: the Official Journal of the World Federation of Hemophilia
Hayley Letson, Geoffrey Dobson
BACKGROUND: Systemic inflammation and coagulopathy are major drivers of injury progression following hemorrhagic trauma. Our aim was to examine the effect of small-volume 3% NaCl adenosine, lidocaine and Mg2+ (ALM) bolus and 0.9% NaCl/ALM 'drip' on inflammation and coagulation in a rat model of hemorrhagic shock. METHODS: Sprague-Dawley rats (429±4 g) were randomly assigned to: 1) shams, 2) no-treatment, 3) saline-controls, 4) ALM-therapy, and 5) Hextend®. Hemorrhage was induced in anesthetized-ventilated animals by liver resection (60% left lateral lobe and 50% medial lobe)...
2017: PloS One
Aliki Papadopoulou, Konstantinos Petrotos, Dimitrios Stagos, Konstantinos Gerasopoulos, Antonios Maimaris, Haralampos Makris, Ioannis Kafantaris, Sotiria Makri, Efthalia Kerasioti, Maria Halabalaki, Vincent Brieudes, Georgia Ntasi, Stylianos Kokkas, Pavlos Tzimas, Panagiotis Goulas, Alexander M Zakharenko, Kirill S Golokhvast, Aristidis Tsatsakis, Demetrios Kouretas
The aim of the study was to examine the effects of a polyphenolic powder from olive mill wastewater (OMWW) administered through drinking water, on chickens' redox status. Thus, 75 chickens were divided into three groups. Group A was given just drinking water, while groups B and C were given drinking water containing 20 and 50 μg/ml of polyphenols, respectively, for 45 days. The antioxidant effects of the polyphenolic powder were assessed by measuring oxidative stress biomarkers in blood after 25 and 45 days of treatment...
2017: Oxidative Medicine and Cellular Longevity
Efthalia Kerasioti, Zoi Terzopoulou, Ourania Komini, Ioannis Kafantaris, Sotiria Makri, Dimitrios Stagos, Konstantinos Gerasopoulos, Nikita Y Anisimov, Aristides M Tsatsakis, Demetrios Kouretas
The aim of the present study was to investigate the effects of livestock feed supplemented with grape pomace (GP) or olive oil mill wastewater (OMW) byproducts on the enzymatic activity and protein expression of antioxidants enzymes, in liver and spleen tissue of sheep. Thus, 36 male sheep of Chios breed were divided into 3 homogeneous groups, control group (n = 12), GP group (n = 12) and OMW group (n = 12), receiving standard or experimental feed. Liver and spleen tissues were collected at 42 and 70 days post-birth...
2017: Toxicology Reports
H Baccouche, A Chakroun, A Zoghlami, S Mahjoub, N Ben Romdhane
WHAT IS KNOWN AND OBJECTIVE: The international normalized ratio (INR) is widely used to monitor patients on vitamin K antagonists. This study aimed to assess the agreement of INR values obtained with different thromboplastin/instrument combinations. MATERIAL AND METHODS: International normalized ratio was determined on plasmas from 330 patients undergoing antivitamin K treatment (with acenocoumarol), using two calibration methods and four reagent/instrument combinations: Both Neoplastine CI and Neoplastine CI Plus on STA-R instrument from Diagnostica STAGO, Asnières, France; and both Thromborel S and Innovin on SYSMEX 2100i instrument from Siemens Health Care Diagnostics, Marbung, Germany...
September 16, 2017: Journal of Clinical Pharmacy and Therapeutics
I U Kusfa, A I Mamman, S M Aminu, A Hassan, H M Muktar
BACKGROUND: Alterations in the components of hemostasis, namely platelet function, the procoagulant, anticoagulant, and the fibrinolytic systems, are observed in sickle cell anemia (SCA) and are in favor of a procoagulant phenotype. Therefore, study of protein C and antithrombin (AT) levels in patients with SCA in steady state may be used in the treatment and/or prevention of SCA-related thrombotic complications. We studied the changes of these naturally occurring anticoagulants in patients with SCA attending the sickle cell clinic in Ahmadu Bello University Teaching Hospital, Zaria...
August 2017: Nigerian Journal of Clinical Practice
Wendy S Baker, Kathleen J Albright, Megan Berman, Heidi Spratt, Peggy A Mann, Jaime Unabia, John R Petersen
BACKGROUND: In this study we examined the difference in patient INR values as measured by the POCT CoaguChek XS device and central laboratory Stago Evolution and Siemens BCS XP analyzers. METHODS: This study composed of 100 warfarin therapy patients and 20 coagulation normal subjects, showed that the difference between the POCT and clinical laboratory values increased with increasing INR and was exacerbated by the use of different thromboplastin reagents by the POCT and central lab...
August 1, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
Paraskevi Kouka, Alexandros Priftis, Dimitrios Stagos, Apostolis Angelis, Panagiotis Stathopoulos, Nikos Xinos, Alexios-Léandros Skaltsounis, Charalampos Mamoulakis, Aristides M Tsatsakis, Demetrios A Spandidos, Demetrios Kouretas
Olive oil (OO) constitutes the basis of the Mediterranean diet, and it seems that its biophenols, such as hydroxytyrosol (HT) may scavenge free radicals, attracting distinct attention due to their beneficial effects in many pathological conditions, such as cancer. To the best of our knowedge, this is the first study in which the functional properties of an OO total polyphenolic fraction (TPF) and pure HT were examined in order to determine their antioxidant effects at a cellular level in endothelial cells and myoblasts...
September 2017: International Journal of Molecular Medicine
I Kafantaris, D Stagos, B Kotsampasi, A Hatzis, A Kypriotakis, K Gerasopoulos, S Makri, N Goutzourelas, C Mitsagga, I Giavasis, K Petrotos, S Kokkas, P Goulas, V Christodoulou, D Kouretas
In the present study, grape pomace (GP) was used as feed additive in the diet of weaned piglets in order to develop innovative feedstuffs and to investigate their potential beneficial effects on welfare, productivity and meat quality. For examining the antioxidant capacity of the experimental feeds, 24 piglets of 20 days old were assigned to two experimental groups receiving standard or experimental diet for 30 days. Blood and tissues collections were performed at four different time-points, 2, 20, 35 and 50 days post birth...
July 17, 2017: Animal: An International Journal of Animal Bioscience
Aleksandra Antovic, Eva-Marie Norberg, Maria Berndtsson, Agnes Rasmuson, Rickard E Malmström, Mika Skeppholm, Jovan Antovic
Laboratory diagnosis of lupus anticoagulant (LA) is based on prolongation in at least one coagulation assay (diluted Russell's viper venom time - dRVVT or activated partial thromboplastin time - aPTT), which normalises after addition of phospholipids. Both assays may be influenced by anticoagulants and therefore LA should not be tested during warfarin or heparin treatment. It has been shown (primarily in vitro) that direct oral anticoagulants (DOACs - dabigatran [DAB], rivaroxaban [RIV] and apixaban [API]) may also influence LA testing...
August 30, 2017: Thrombosis and Haemostasis
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