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https://www.readbyqxmd.com/read/28959661/tissue-specific-effects-of-feeds-supplemented-with-grape-pomace-or-olive-oil-mill-wastewater-on-detoxification-enzymes-in-sheep
#1
Efthalia Kerasioti, Zoi Terzopoulou, Ourania Komini, Ioannis Kafantaris, Sotiria Makri, Dimitrios Stagos, Konstantinos Gerasopoulos, Nikita Y Anisimov, Aristides M Tsatsakis, Demetrios Kouretas
The aim of the present study was to investigate the effects of livestock feed supplemented with grape pomace (GP) or olive oil mill wastewater (OMW) byproducts on the enzymatic activity and protein expression of antioxidants enzymes, in liver and spleen tissue of sheep. Thus, 36 male sheep of Chios breed were divided into 3 homogeneous groups, control group (n = 12), GP group (n = 12) and OMW group (n = 12), receiving standard or experimental feed. Liver and spleen tissues were collected at 42 and 70 days post-birth...
2017: Toxicology Reports
https://www.readbyqxmd.com/read/28921594/the-international-normalized-ratio-inr-what-reagent-what-instrument-the-assessment-of-the-agreement-between-inr-values-according-to-different-reagent-instrument-combinations
#2
H Baccouche, A Chakroun, A Zoghlami, S Mahjoub, N Ben Romdhane
WHAT IS KNOWN AND OBJECTIVE: The international normalized ratio (INR) is widely used to monitor patients on vitamin K antagonists. This study aimed to assess the agreement of INR values obtained with different thromboplastin/instrument combinations. MATERIAL AND METHODS: International normalized ratio was determined on plasmas from 330 patients undergoing antivitamin K treatment (with acenocoumarol), using two calibration methods and four reagent/instrument combinations: Both Neoplastine CI and Neoplastine CI Plus on STA-R instrument from Diagnostica STAGO, Asnières, France; and both Thromborel S and Innovin on SYSMEX 2100i instrument from Siemens Health Care Diagnostics, Marbung, Germany...
September 16, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/28891545/protein-c-and-antithrombin-levels-in-patients-with-sickle-cell-anemia-in-ahmadu-bello-university-teaching-hospital-zaria-nigeria
#3
I U Kusfa, A I Mamman, S M Aminu, A Hassan, H M Muktar
BACKGROUND: Alterations in the components of hemostasis, namely platelet function, the procoagulant, anticoagulant, and the fibrinolytic systems, are observed in sickle cell anemia (SCA) and are in favor of a procoagulant phenotype. Therefore, study of protein C and antithrombin (AT) levels in patients with SCA in steady state may be used in the treatment and/or prevention of SCA-related thrombotic complications. We studied the changes of these naturally occurring anticoagulants in patients with SCA attending the sickle cell clinic in Ahmadu Bello University Teaching Hospital, Zaria...
August 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28774502/poct-pt-inr-is-it-adequate-for-patient-care-a-comparison-of-the-roche-coaguchek-xs-vs-stago-star-vs-siemens-bcs-in-patients-routinely-seen-in-an-anticoagulation-clinic
#4
Wendy S Baker, Kathleen J Albright, Megan Berman, Heidi Spratt, Peggy A Mann, Jaime Unabia, John R Petersen
BACKGROUND: In this study we examined the difference in patient INR values as measured by the POCT CoaguChek XS device and central laboratory Stago Evolution and Siemens BCS XP analyzers. METHODS: This study composed of 100 warfarin therapy patients and 20 coagulation normal subjects, showed that the difference between the POCT and clinical laboratory values increased with increasing INR and was exacerbated by the use of different thromboplastin reagents by the POCT and central lab...
August 1, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28731131/assessment-of-the-antioxidant-activity-of-an-olive-oil-total-polyphenolic-fraction-and-hydroxytyrosol-from-a-greek-olea%C3%A2-europea-variety-in-endothelial-cells-and-myoblasts
#5
Paraskevi Kouka, Alexandros Priftis, Dimitrios Stagos, Apostolis Angelis, Panagiotis Stathopoulos, Nikos Xinos, Alexios-Léandros Skaltsounis, Charalampos Mamoulakis, Aristides M Tsatsakis, Demetrios A Spandidos, Demetrios Kouretas
Olive oil (OO) constitutes the basis of the Mediterranean diet, and it seems that its biophenols, such as hydroxytyrosol (HT) may scavenge free radicals, attracting distinct attention due to their beneficial effects in many pathological conditions, such as cancer. To the best of our knowedge, this is the first study in which the functional properties of an OO total polyphenolic fraction (TPF) and pure HT were examined in order to determine their antioxidant effects at a cellular level in endothelial cells and myoblasts...
September 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28712376/grape-pomace-improves-performance-antioxidant-status-fecal-microbiota-and-meat-quality-of-piglets
#6
I Kafantaris, D Stagos, B Kotsampasi, A Hatzis, A Kypriotakis, K Gerasopoulos, S Makri, N Goutzourelas, C Mitsagga, I Giavasis, K Petrotos, S Kokkas, P Goulas, V Christodoulou, D Kouretas
In the present study, grape pomace (GP) was used as feed additive in the diet of weaned piglets in order to develop innovative feedstuffs and to investigate their potential beneficial effects on welfare, productivity and meat quality. For examining the antioxidant capacity of the experimental feeds, 24 piglets of 20 days old were assigned to two experimental groups receiving standard or experimental diet for 30 days. Blood and tissues collections were performed at four different time-points, 2, 20, 35 and 50 days post birth...
July 17, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28640321/effects-of-direct-oral-anticoagulants-on-lupus-anticoagulant-assays-in-a-real-life-setting
#7
Aleksandra Antovic, Eva-Marie Norberg, Maria Berndtsson, Agnes Rasmuson, Rickard E Malmström, Mika Skeppholm, Jovan Antovic
Laboratory diagnosis of lupus anticoagulant (LA) is based on prolongation in at least one coagulation assay (diluted Russell's viper venom time - dRVVT or activated partial thromboplastin time - aPTT), which normalises after addition of phospholipids. Both assays may be influenced by anticoagulants and therefore LA should not be tested during warfarin or heparin treatment. It has been shown (primarily in vitro) that direct oral anticoagulants (DOACs - dabigatran [DAB], rivaroxaban [RIV] and apixaban [API]) may also influence LA testing...
August 30, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28613098/combined-treatment-of-irbesartan-and-diltiazem-ameliorates-endothelium-dependent-vasodilatation-in-hypertensives
#8
Tingting Zhou, Xiaodong Huang, Xiaoqi Cai, Liangdi Xie
OBJECTIVE: Endothelial function is of prognostic importance for hypertensives. The aim of this study was to investigate the effects of irbesartan combined with diltiazem on the endothelium-dependent vasodilatation in essential hypertensive (EH) patients in China. METHODS: A total of 150 Chinese hypertensives aged from 40 to 80 years old were assigned into three groups: irbesartan treated(150 mg/d, n = 46), diltiazem treated (90 mg/d, n = 51), and combined therapy group (irbesartan 150 mg/d+ diltiazem 90 mg/d, n = 53)...
June 14, 2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/28565852/oxidation-of-human-serum-albumin-exhibits-inter-individual-variability-after-an-ultra-marathon-mountain-race
#9
Ypatios Spanidis, Alexandros Priftis, Dimitrios Stagos, George A Stravodimos, Demetres D Leonidas, Demetrios A Spandidos, Aristides M Tsatsakis, Demetrios Kouretas
The aim of this study was to examine the oxidation of human serum albumin (HSA) caused by oxidative stress following exhaustive and demanding exercise, such as an ultra-marathon race. For this purpose, blood samples from 12 adult runners who underwent a 103 km mountain ultra-marathon race were collected before the race, and also at 24, 48 and 72 h post-race. HSA was partially purified using affinity chromatography and consequently subjected to western blot analysis in order to determine the levels of disulfide dimers indicating oxidation...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28549386/newly-developed-dilute-russell-s-viper-venom-reagents-for-lupus-anticoagulant-detection-with-improved-specificity
#10
G W Moore, M Peyrafitte, C Dunois, J Amiral
Background Dilute Russell's viper venom time (dRVVT) is indispensible in lupus anticoagulant (LA) detection yet commercial reagents from different suppliers perform variably, no gold standard assays exist and therapeutic anticoagulation interference is problematic. Objective The objective of this study was to compare a new formulation dRVVT with two currently available dRVVTs. Materials and methods Life Diagnostics (LD) dRVVT and Stago PTT-LA were routinely used for lupus anticoagulant detection, plus Taipan snake venom time/ecarin time (TSVT/ET) for patients on warfarin or rivaroxaban...
January 1, 2017: Lupus
https://www.readbyqxmd.com/read/28534833/coagulating-colubrids-evolutionary-pathophysiological-and-biodiscovery-implications-of-venom-variations-between-boomslang-dispholidus-typus-and-twig-snake-thelotornis-mossambicanus
#11
Jordan Debono, James Dobson, Nicholas R Casewell, Anthony Romilio, Bin Li, Nyoman Kurniawan, Karine Mardon, Vera Weisbecker, Amanda Nouwens, Hang Fai Kwok, Bryan G Fry
Venoms can deleteriously affect any physiological system reachable by the bloodstream, including directly interfering with the coagulation cascade. Such coagulopathic toxins may be anticoagulants or procoagulants. Snake venoms are unique in their use of procoagulant toxins for predatory purposes. The boomslang (Dispholidus typus) and the twig snakes (Thelotornis species) are iconic African snakes belonging to the family Colubridae. Both species produce strikingly similar lethal procoagulant pathologies. Despite these similarities, antivenom is only produced for treating bites by D...
May 19, 2017: Toxins
https://www.readbyqxmd.com/read/28412717/fibrinogen-determination-according-to-clauss-commutability-assessment-of-international-and-commercial-standards-and-quality-control-samples
#12
Antonius M H P van den Besselaar, Claudia J J van Rijn, Christa M Cobbaert, G Louis A Reijnierse, Martine J Hollestelle, René W L M Niessen, Francisca Hudig
BACKGROUND: Many clinical laboratories use a clotting rate assay according to Clauss for the determination of fibrinogen in citrated plasma. The aim of the present study was to assess the commutability of the current International Standard for fibrinogen (coded 09/264), three commercial fibrinogen standards, and 10 freeze-dried plasma quality control samples from various sources. METHODS: Clotting rate assays according to Clauss were performed on three automated instruments (Sysmex CA1500, STA-Rack Evolution and ACL-Top 700), using three commercial thrombin reagents (Siemens, Stago, and Instrumentation Laboratory)...
October 26, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28318109/agreement-between-factor-xiii-activity-and-antigen-assays-in-measurement-of-factor-xiii-a-french-multicenter-study-of-147-human-plasma-samples
#13
C Caron, R Meley, V Le Cam Duchez, M F Aillaud, C Lavenu-Bombled, F Dutrillaux, C Flaujac, A Ryman, C Ternisien, D Lasne, H Galinat, C Pouplard
INTRODUCTION: Factor XIII (FXIII) deficiency is a rare hemorrhagic disorder whose early diagnosis is crucial for appropriate treatment and prophylactic supplementation in cases of severe deficiency. International guidelines recommend a quantitative FXIII activity assay as first-line screening test. FXIII antigen measurement may be performed to establish the subtype of FXIII deficiency (FXIIID) when activity is decreased. METHODS: The aim of this multicenter study was to evaluate the analytical and diagnostic levels of performance of a new latex immunoassay, K-Assay(®) FXIII reagent from Stago, for first-line measurement of FXIII antigen...
March 20, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28316157/-the-diagnostic-value-of-plasma-fibrin-related-markers-in-perioperation-of-femoral-fracture
#14
J Li, Z Chen, L H Zhang, Z L Li, D M Wu, S S Wang, L J Xu, H Xu, P F Tang, C B Wang
Objective: To investigate potential value of fibrin related markers in patients with femoral fracture during perioperative period. Methods: Ninety-five patients were enrolled, including 39 males with (53±24) years old and 56 females with (73±13) years old, of which 44 fracture on caput femoris, 34 collum femoris and 17 shaft of femur. Sampling on the day before operation and 1(st,) 3(rd,) 5(th) days after operation, fibrin monomer (FM), D-dimer(DD), fibrinogen degradation product (FDP) and other coagulation assays were detected by reagents from Stago...
March 14, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28291473/physiological-impact-of-platelet-apheresis-in-pigs-oxygen-metabolism-and-coagulation
#15
Wenjun Z Martini, Cassandra M Rodriguez, Jonathan Richardson, James K Aden, Andrew P Cap, Michael A Dubick
INTRODUCTION: Platelet apheresis is a routine clinical practice, but the physiological impact on the donors has been incompletely characterized. This study measured the effects of platelet apheresis on hemodynamics, oxygen metabolism, and coagulation in pigs to assess its impact before employing the animals in experimental studies. METHODS: Forty pigs (39.8 ± 0.6 kg) were anesthetized and catheterized with an apheresis catheter in the femoral vein. During the platelet apheresis process, blood was withdrawn from the pig to separate platelets, and the remaining red blood cells and plasma returned back to the pigs, using the Haemonetics MCS+9000 system...
March 2017: Military Medicine
https://www.readbyqxmd.com/read/28260086/development-of-an-assay-to-assess-genotoxicity-by-particulate-matter-extract
#16
Alexandros Priftis, Konstantinos Papikinos, Marina Koukoulanaki, Efthalia Kerasioti, Dimitrios Stagos, Konstantinos Konstantinopoulos, Demetrios A Spandidos, Marianthi Kermenidou, Spyros Karakitsios, Dimosthenis Sarigiannis, Aristides M Tsatsakis, Demetrios Kouretas
The current study describes a method for assessing the oxidative potential of common environmental stressors (ambient air particulate matter), using a plasmid relaxation assay where the extract caused single-strand breaks, easily visualised through electrophoresis. This assay utilises a miniscule amount (11 µg) of particulate matter (PM) extract compared to other, cell‑based methods (~3,000 µg). The negative impact of air pollution on human health has been extensively recognised. Among the air pollutants, PM plays an eminent role, as reflected in the broad scientific interest...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28212265/variations-in-oxidative-stress-levels-in-3-days-follow-up-in-ultramarathon-mountain-race-athletes
#17
Ypatios Spanidis, Dimitrios Stagos, Marina Orfanou, Nikolaos Goutzourelas, David Bar-Or, Demetrios Spandidos, Demetrios Kouretas
Spanidis, Y, Stagos, D, Orfanou, M, Goutzourelas, N, Bar-or, D, Spandidos, D, and Kouretas, D. Variations in oxidative stress levels in 3 days follow-up in ultramarathon mountain race athletes. J Strength Cond Res 31(3): 582-594, 2017-The aim of the present study was the monitoring of the redox status of runners participating in a mountain ultramarathon race of 103 km. Blood samples from 12 runners were collected prerace and 24, 48, and 72 hours postrace. The samples were analyzed by using conventional oxidative stress markers, such as protein carbonyls (CARB), thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC) in plasma, as well as glutathione (GSH) levels and catalase (CAT) activity in erythrocytes...
March 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28150507/the-microinr-portable-coagulometer-analytical-quality-and-user-friendliness-of-a-pt-inr-point-of-care-instrument
#18
Pia Bükmann Larsen, Elin Storjord, Åsne Bakke, Tone Bukve, Mikael Christensen, Joakim Eikeland, Vegar Engeland Haugen, Kristin Husby, Rie McGrail, Solveig Meier Mikaelsen, Grete Monsen, Mette Fogh Møller, Jan Nybo, Jesper Revsholm, Aslaug Johanne Risøy, Unni Marie Skålsvik, Heidi Strand, Reyes Serrano Teruel, Elvar Theodorsson
Regular measurement of prothrombin time as an international normalized ratio PT (INR) is mandatory for optimal and safe use of warfarin. Scandinavian evaluation of laboratory equipment for primary health care (SKUP) evaluated the microINR portable coagulometer (microINR(®)) (iLine Microsystems S.L., Spain) for measurement of PT (INR). Analytical quality and user-friendliness were evaluated under optimal conditions at an accredited hospital laboratory and at two primary health care centres (PHCCs). Patients were recruited at the outpatient clinic of the Laboratory of Medical Biochemistry, St Olav's University Hospital, Trondheim, Norway (n = 98) and from two PHCCs (n = 88)...
April 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28133956/development-of-a-novel-automated-screening-method-for-detection-of-fviii-inhibitors
#19
M S Evans, K J Donaldson, M E Eyster
INTRODUCTION: Factor VIII activity is routinely determined by measuring the activated partial thromboplastin time (aPTT) of a patient plasma sample and determining percent activity from a standard curve. To maximize the detection of a clotting factor inhibitor, a subjective assessment of parallelism of a patient curve compared with a standard curve is performed. We developed and validated an automated objective method to assess parallelism as a rapid screening tool for detection of an inhibitor to factor VIII during routine FVIII assays...
April 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28130089/novel-feed-including-bioactive-compounds-from-winery-wastes-improved-broilers-redox-status-in-blood-and-tissues-of-vital-organs
#20
Sotiria Makri, Ioannis Kafantaris, Dimitrios Stagos, Theodora Chamokeridou, Konstantinos Petrotos, Konstantinos Gerasopoulos, Anastasios Mpesios, Nikolaos Goutzourelas, Stylianos Kokkas, Panagiotis Goulas, Dimitrios Komiotis, Dimitrios Kouretas
Currently, there is a great interest in the production of animal feed with antioxidant activity. The aim of this study was to examine the potential antioxidant effects of a feed supplemented with grape pomace (GP), a winery by-product with high environmental load, in chickens. Broilers of 15 days post birth were separated into two groups fed either with standard diet or with diet supplemented with GP for 35 days. Blood and tissues collections were performed after feeding for 15 and 35 days with the experimental diet (i...
April 2017: Food and Chemical Toxicology
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