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Leyla Calmette, Nicole Charpentier, Caroline Tircot, Delphine Bigot, Claire Dunois, Jean Amiral, Marcelle Tetegan, Sonnthida Sep Hieng, Jean-Yves Peltier
Our study aimed to evaluate and validate according to standard NF EN ISO 15189 the original protocol ajustement of Hemoclot Protein C (PC) (Hyphen BioMed), clotting-based assay of PC on ACL TOP analyzer (Werfen/Instrumentation Laboratory). We evaluated the performance in terms of imprecision and we validate additional parameters in range B required by the SH GTA 04 (COFRAC): repeatability, reproducibility, detection and quantification limits, limits of linearity, stability, inter-samples and inter-reagents contamination, inaccuracy, evaluation of interferences (hemolysis, bilirubinemia and chyles)...
December 1, 2016: Annales de Biologie Clinique
Ming Sheng Lim, Kent Chapman, Priscilla Swanepoel, Anoop K Enjeti
Most studies on the sensitivities of coagulation assays to direct oral anticoagulants (DOACs) are based on normal plasma spiked with anticoagulant in the laboratory. Recent studies have shown that reagent sensitivity varies significantly depending on whether spiked or patient samples are used. The aim of this study was to compare the sensitivities of routine coagulation assays in patient samples and commercial drug specific calibrators using commonly used activated partial thromboplastin time (APTT) and prothrombin time (PT) reagents (i...
December 2016: Pathology
I Kafantaris, B Kotsampasi, V Christodoulou, E Kokka, P Kouka, Z Terzopoulou, K Gerasopoulos, D Stagos, C Mitsagga, I Giavasis, S Makri, K Petrotos, D Kouretas
Grape pomace is a by-product of winemaking process and rich in bioactive compounds such as plant polyphenols having antioxidant and antimicrobial activities. As known, oxidative stress may cause a number of pathological conditions in farm animals and thus affecting animal welfare and production. Moreover, pathogenic bacteria affect animals' health status. Thus, the objective of this study was to investigate whether lambs' feed supplemented with grape pomace enhances the antioxidant mechanisms and reduces the growth of pathogenic bacteria...
October 18, 2016: Journal of Animal Physiology and Animal Nutrition
S Louw, N P Saragas, P N Ferrao, T F Chirwa, B F Jacobson
BACKGROUND: Low-molecular-weight heparin and vitamin K antagonists such as warfarin are the gold standard for prohylaxis and treatment of venous thromboembolic disease (VTED). Direct oral anticoagulants (DOACs) result in predictable anticoagulation with significantly reduced inter- and intra-patient variability. DOAC absorption is rapid, with a short half-life and relatively few drug interactions. DOACs are effective and safe at fixed doses without activity monitoring. However, specific situations may require assessment of accurate drug activity...
September 8, 2016: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
Alexandros Priftis, Dimitrios Stagos, Nikolaos Tzioumakis, Konstantinos Konstantinopoulos, Anastasia Patouna, Georgios E Papadopoulos, Aristides Tsatsakis, Dimitrios Kouretas
Measuring the antioxidant capacity of foods is essential, as a means of quality control to ensure that the final product reaching the consumer will be of high standards. Despite the already existing assays with which the antioxidant activity is estimated, new, faster and low cost methods are always sought. Therefore, we have developed a novel colorimeter and combined it with a slightly modified DPPH assay, thus creating a kit that can assess the antioxidant capacity of liquids (e.g., different types of coffee, beer, wine, juices) in a quite fast and low cost manner...
August 30, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Efthalia Kerasioti, Dimitrios Stagos, Aggeliki Tzimi, Dimitrios Kouretas
The aim of the present study was to investigate the molecular mechanisms through which sheep/goat whey protein exerts its antioxidant activity. Thus, it was examined whey protein's effects on the expression of transcription factor, nuclear factor-like 2 (Nrf2) and on the expression and activity of a number of antioxidant and phase II enzymes, superoxide dismutase (SOD), catalase (CAT), heme oxygenase 1 (HO-1), synthase glutamyl cysteine (GCS) and glutathione-s-transferase (GST), in muscle C2C12 and EA.hy926 endothelial cells...
November 2016: Food and Chemical Toxicology
Spanidis Ypatios, Stagos Dimitrios, Orfanou Marina, Goutzourelas Nikolaos, Bar-Or David, Spandidos Demetrios, Kouretas Demetrios
The aim of the present study was the monitoring of the redox status of runners participating in a mountain ultra-marathon race of 103 km. Blood samples from 12 runners were collected pre race and 24, 48 and 72 h post race. The samples were analyzed by using conventional oxidative stress markers, such as protein carbonyls (CARB), thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC) in plasma, as well as glutathione (GSH) levels and catalase (CAT) activity in erythrocytes. In addition, two novel markers the static oxidation-reduction potential marker (sORP) and the capacity oxidation-reduction potential (cORP) were measured in plasma...
July 25, 2016: Journal of Strength and Conditioning Research
Barbara Depreter, Katrien M J Devreese
BACKGROUND: Lupus anticoagulant (LAC) detection represents diagnostic challenges among which the multitude of available reagents and interference by anticoagulant treatment. One of the two advised tests is the dilute Russell's viper venom time (dRVVT). However, it is currently not clear whether all dRVVT reagents may be considered equivalent. The objective of the study was to evaluate the diagnostic performance of two dRVVT reagents, with special attention to the influence of anticoagulant therapy...
January 1, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
A Woolley, J-L Golmard, S Kitchen
INTRODUCTION: Haemolysis, icterus and lipaemia (HIL) may affect haemostasis test results. This may be influenced by the level of interfering substance and the reagents and end-point detection system used. METHODS: We assessed the influence of HIL on prothrombin time, activated partial thromboplastin time and fibrinogen assay using a viscosity-based detection analyser. RESULTS: Spontaneous haemolysis that occurred during sample collection and processing had no effect on PT with either a rabbit tissue factor extract or recombinant human tissue factor reagents...
August 2016: International Journal of Laboratory Hematology
Cécile Florin, Olivier Garraud, Serge Molliex, Brigitte Tardy, Lydia Campos, Carine Scherrer
The Innovance VWF:Ac test (Siemens) has the particularity to assess the binding capacity of von Willebrand factor (VWF) to recombinant platelet GPIb mutated in the absence of ristocetin. Our study aimed to evaluate and validate according to standard NF EN ISO 15189 the original protocol adaptation on STA-R Evolution series analyser (Diagnostica Stago). We evaluated the performance in terms of imprecision and we validate additional parameters necessary in range B as recommended by the SH GTA 04 (Cofrac). We compared the new assay with the reference assay: ristocetin cofactor activity (VWF:RCo) performed on the BCS-XP analyser by testing retrospectively samples from 82 healthy normal subjects and 61 patients with von Willebrand disease (VWD)...
June 1, 2016: Annales de Biologie Clinique
Efthalia Kerasioti, Dimitrios Stagos, Vasiliki Georgatzi, Erinda Bregou, Alexandros Priftis, Ioannis Kafantaris, Dimitrios Kouretas
Excessive production of reactive oxygen species (ROS) may cause endothelial dysfunction and consequently vascular disease. In the present study, the possible protective effects of sheep whey protein (SWP) from tert-butyl hydroperoxide- (tBHP-) induced oxidative stress in endothelial cells (EA.hy926) were assessed using oxidative stress biomarkers. These oxidative stress biomarkers were glutathione (GSH) and ROS levels determined by flow cytometry. Moreover, thiobarbituric acid-reactive substances (TBARS), protein carbonyls (CARB), and oxidized glutathione (GSSG) were determined spectrophotometrically...
2016: Oxidative Medicine and Cellular Longevity
Konstantinos Gerasopoulos, Dimitrios Stagos, Alexandros Krouezas, Christina Karaveli, Christina Barda, Helen Gkika, Dimitrios Mitsiou, Konstantinos Petrotos, Panagiotis Goulas, Demetrios Kouretas
BACKGROUND/AIM: A previous study revealed the improvement of redox status in blood and tissues of young piglets (ablactation period), that consumed feed containing polyphenolic additives from byproducts of processed olive mill wastewater (OMWW). The polyphenolic additives strengthened the antioxidant defense of the piglets. Herein we analyzed the fatty acid (FA) composition of these animals in various tissues. MATERIALS AND METHODS: The steps followed during the analysis were: Preparation and isolation of byproducts containing polyphenolic compounds from OMWW processing, silage and piglet feed preparation, blood and tissue collection, fatty acid methyl esters synthesis and GC/MS analysis...
May 2016: In Vivo
Ypatios Spanidis, Anastasios Mpesios, Dimitrios Stagos, Nikolaos Goutzourelas, David Bar-Or, Maria Karapetsa, Epaminondas Zakynthinos, Demetrios A Spandidos, Aristides M Tsatsakis, George Leon, Demetrios Kouretas
The aim of the present study was to examine the effectiveness of a new redox status marker, the static oxidation reduction potential (sORP), for assessing oxidative stress in 75 patients with metabolic syndrome (MetS) and type 2 diabetes (T2D). A total of 35 normal subjects were used as the controls. Moreover, conventional markers of oxidative stress were assessed, such as thiobarbituric acid reactive substances (TBARS), protein carbonyls, the total antioxidant capacity in plasma, glutathione (GSH) levels and catalase (CAT) activity in erythrocytes...
March 2016: Experimental and Therapeutic Medicine
A H Kristoffersen, T K Bjånes, S Jordal, S Leh, F Leh, E Svarstad
Unexpectedly prolonged activated partial thromboplastin times (APTTs) were noted in several patients with chronic renal insufficiency and a history of intravenous drug abuse. Deposits of polyvinylpyrrolidone (PVP), an excipient in one oral methadone solution used in opioid maintenance programs, were found in renal biopsies. One case is described in detail, followed by a summary of findings in 11 other patients and results of an in vitro experiment where plasma was spiked with PVP at increasing concentrations...
February 26, 2016: Journal of Thrombosis and Haemostasis: JTH
E L Benade, B F Jacobson, S Louw, E Schapkaitz
BACKGROUND: Measurement of the international normalised ratio (INR) is essential in the management of patients on long-term warfarin therapy. The CoaguChek XS portable coagulometer is a point-of-care test for INR measurement. It offers the advantage of improved patient accessibility, particularly in peripheral clinics. OBJECTIVES: To evaluate the clinical utility of the CoaguChek XS for monitoring of patients on standard warfarin therapy (INR 2 - 3) as well as those with mechanical heart valve replacements (INR 2...
March 2016: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
Ypatios Spanidis, Nikolaos Goutzourelas, Dimitrios Stagos, Anastasios Mpesios, Alexandros Priftis, David Bar-Or, Demetrios A Spandidos, Aristides M Tsatsakis, George Leon, Demetrios Kouretas
The aim of the present study was to examine the changes occuring in the redox status in male basketball players at the beginning and end of a highly competitive season. For this purpose, the redox status of 14 professional athletes of a European basketball club was examined at 2 different time points, at the beginning (phase 1) and at the end of the season (phase 2). The redox status was assessed in blood using conventional oxidative stress markers, such as thiobarbituric acid reactive substances (TBARS), protein carbonyls (CARB) and the total antioxidant capacity (TAC) in plasma, as well as glutathione (GSH) levels and catalase (CAT) activity in erythrocytes...
January 2016: Experimental and Therapeutic Medicine
Wenbin Zhou, Chenbin Li, Haipeng Zhang, Fei Cheng, Mingting Peng
OBJECTIVE: To evaluate the comparability of measurement results for coagulation factor VIII (FVIII)and factor IX (FIX) activity and the commutability of reference materials on different measurement systems. METHODS: The study was performed according to CLSI guideline EP30 and China health standard WS/T 356-2011. Clinical samples with different levels of FVIII and FIX which covered over the clinical analytical range, five lots of homemade reference materials (F20140601-F20140605) and a coagulation reference material (SSCLOT4) provided by NIBSC were detected for FVIII and FIX activity on three popular measurement systems in China, which including Stago STA-R Evolution, IL ACL TOP700 and Sysmex CA7000 automatic coagulation analyzers using supplementary reagents...
September 8, 2015: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Konstantinos Gerasopoulos, Dimitrios Stagos, Konstantinos Petrotos, Stylianos Kokkas, Dimitrios Kantas, Panagiotis Goulas, Dimitrios Kouretas
In the present study, a polyphenolic byproduct from olive mill wastewater (OMWW) was used for making piglet feed with antioxidant activity. For examining the antioxidant capacity of the feed, 30 piglets of 20 d old were divided into two groups receiving basal or experimental feed for 30 d. Blood and tissue samples were drawn at days 2, 20, 35 and 50 post-birth. The tissues collected were brain, heart, kidney, liver, lung, quadriceps muscle, pancreas, spleen and stomach. The antioxidant effects of the experimental feed were assessed by measuring oxidative stress biomarkers in blood and tissues...
December 2015: Food and Chemical Toxicology
Alexandros Priftis, Dimitrios Stagos, Konstantinos Konstantinopoulos, Christina Tsitsimpikou, Demetrios A Spandidos, Aristides M Tsatsakis, Manolis N Tzatzarakis, Demetrios Kouretas
Coffee is one of the most popular and widely consumed beverages worldwide due to its pleasant taste and aroma. A number of studies have been performed to elucidate the possible beneficial effects of coffee consumption on human health and have shown that coffee exhibits potent antioxidant activity, which may be attributed mainly to its polyphenolic content. However, there is also evidence to suggest that coffee roasting (the procedure which turns green coffee beans to the dark, roasted ones from which the beverage derives) may alter the polyphenolic profile of the beans (e...
November 2015: Molecular Medicine Reports
Ypatios Spanidis, Nikolaos Goutzourelas, Dimitrios Stagos, Anastasia S Kolyva, Charalambos A Gogos, David Bar-Or, Dimitrios Kouretas
BACKGROUND/AIM: The novel static (sORP) and capacity (cORP) oxidation-reduction potential markers were examined for assessing oxidative stress in plasma of patients with sepsis. Moreover, the possible effect of obesity-induced oxidative stress on patients with sepsis was investigated. MATERIALS AND METHODS: sORP and cORP markers, as well as the conventional oxidative stress biomarkers total antioxidant capacity (TAC), thiobarbituric acid-reactive substances (TBARS) and protein carbonyls (CARB), were assessed in plasma...
September 2015: In Vivo
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