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https://www.readbyqxmd.com/read/28732383/radiation-alters-pd-l1-nkg2d-ligand-levels-in-lung-cancer-cells-and-leads-to-immune-escape-from-nk-cell-cytotoxicity-via-il-6-mek-erk-signaling-pathway
#1
Ming Jing Shen, Li Jun Xu, Li Yang, Ying Tsai, Peter C Keng, Yongbing Chen, Soo Ok Lee, Yuhchyau Chen
We investigated whether radiation influences the susceptibility of non-small cell lung cancer (NSCLC) cells to NK cell mediated cytotoxicity. We found radiation treatment increased expression of programmed cell death ligand 1 (PD-L1), but decreased NK group 2, member D (NKG2D) ligand expressions in A549 and H157 NSCLC cells. Both types of changes would have protected tumor cells from the cytotoxic action of NK cells. Consistently, we detected similar alteration in these molecules in radioresistant A549R26-1 and H157R24-1 subline cells...
July 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28732241/generation-of-skeletal-myogenic-progenitors-from-human-pluripotent-stem-cells-using-non-viral-delivery-of-minicircle-dna
#2
Jaemin Kim, Vanessa K P Oliveira, Ami Yamamoto, Rita C R Perlingeiro
Currently, the most efficient and promising approach for generating large numbers of engraftable human skeletal myogenic progenitors from pluripotent stem cells requires the conditional in vitro overexpression of PAX7 using lentiviral vectors. Because a non-integrating approach would be preferable to eliminate or minimize the risk associated with random genomic integration, here we investigate whether transient expression of PAX7 using minicircle DNA would enable the generation of functional pluripotent stem cell-derived myogenic progenitors, equivalent to those generated by lentivirus...
July 12, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28732234/computational-modelling-of-genome-scale-metabolic-networks-and-its-application-to-cho-cell-cultures
#3
REVIEW
Živa Rejc, Lidija Magdevska, Tilen Tršelič, Timotej Osolin, Rok Vodopivec, Jakob Mraz, Eva Pavliha, Nikolaj Zimic, Tanja Cvitanović, Damjana Rozman, Miha Moškon, Miha Mraz
Genome-scale metabolic models (GEMs) have become increasingly important in recent years. Currently, GEMs are the most accurate in silico representation of the genotype-phenotype link. They allow us to study complex networks from the systems perspective. Their application may drastically reduce the amount of experimental and clinical work, improve diagnostic tools and increase our understanding of complex biological phenomena. GEMs have also demonstrated high potential for the optimisation of bio-based production of recombinant proteins...
July 8, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28732210/nursing-as-universal-and-recognisable-nursing-students-perceptions-of-learning-outcomes-from-intercultural-peer-learning-webinars-a-qualitative-study
#4
Elisabeth Carlson, Marie Stenberg, Bessie Chan, Sukki Ho, Timothy Lai, Arkers Wong, Engle Angela Chan
Nursing students need to be prepared for the increasingly culturally diverse health care. Therefore, providing students with international perspectives remains the mission of higher education. However, given the logistic and financial constraints, not all students will be able to travel overseas for their international experiences. A feasible alternative to study abroad is internationalisation-at-home where intercultural dimensions are incorporated into curriculum, without students leaving their home universities...
July 15, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/28732200/systematic-metrics-depicting-cell-death-kinetics
#5
Ying Li, Junying Yuan
In a recent issue of Cell Systems, Forcina et al. (2017) developed a scalable time-lapse analysis of cell death kinetics (STACK) method and combined it with a "lag exponential death" model to quantitatively characterize the onset and rate of cell death. STACK provides a useful quantitative tool to determine how cell death kinetics may be modulated pharmacologically in cell culture systems.
July 20, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28732179/kgf-enhances-oral-epithelial-adhesion-and-rete-peg-elongation-via-integrins
#6
G L Sa, X P Xiong, J G Ren, J Y Wang, H F Xia, Z K Liu, S G He, Y F Zhao
Oral epithelial adhesion to the lamina propria underlies the physiologic function of the oral mucosa and contributes to resisting bacterial invasion, preventing body fluid loss, and maintaining routine chewing; thus, understanding the factors that positively influence oral epithelial adhesion is a research topic of great interest. Rete pegs contribute to oral epithelial adhesion by enlarging the contact areas, whereas integrins are the major molecules that mediate epithelial cell adhesion to the basement membrane...
July 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28732126/chinese-isms-dimensions-in-mainland-china-and-taiwan-convergence-and-extension-of-american-isms-dimensions
#7
Zhuo Job Chen, Kung-Yu Hsu, Xinyue Zhou, Gerard Saucier
OBJECTIVE: Previous studies of American-English isms terms have uncovered as many as five broad factors: Tradition-oriented Religiousness (TR), Subjective Spirituality (SS), Communal Rationalism (CR), Unmitigated Self-Interest (USI), and Inequality Aversion (IA). The present studies took a similar lexical approach to investigate the Chinese-language isms structures in both mainland China and Taiwan. METHOD AND RESULTS: In Study 1, exploratory factor analyses with 915 mainland Chinese subjects uncovered four interpretable factors dimensionalizing 165 mainland Chinese dictionary isms terms...
July 21, 2017: Journal of Personality
https://www.readbyqxmd.com/read/28732028/the-individual-and-contextual-determinants-of-the-use-of-telemedicine-a-descriptive-study-of-the-perceptions-of-senegal-s-physicians-and-telemedicine-projects-managers
#8
Birama Apho Ly, Ronald Labonté, Ivy Lynn Bourgeault, Mbayang Ndiaye Niang
Telemedicine is considered to be an effective strategy to aid in the recruitment and retention of physicians in underserved areas and, in doing so, improve access to healthcare. Telemedicine's use, however, depends on individual and contextual factors. Using a mixed methods design, we studied these factors in Senegal based on a micro, meso and macro framework. A quantitative questionnaire administered to 165 physicians working in public hospitals and 151 physicians working in district health centres was used to identify individual (micro) factors...
2017: PloS One
https://www.readbyqxmd.com/read/28731923/human-bocavirus-can-it-trigger-hemophagocytic-lymphohistiocytosis
#9
Sevgen Tanir Basaranoglu, Kubra Aykac, Yasemin Ozsurekci, Inci Bajin, Betul Tavil, Fatma Gumruk, Mehmet Ceyhan
With improvements in molecular diagnostic methods, report of Human bocavirus (HBoV) as an etiologic agent in many studies on viral respiratory and gastrointestinal infections has been increasing. Two pediatric patients who presented with secondary hemophagocytic lymphohistiocytosis were examined for etiologic causes, including viruses. Whole bacterial and fungal cultures and viral serological studies were negative. Viral polymerase chain reaction of nasopharyngeal secretions showed HBoV. One was successfully treated with intravenous immunoglobulins, whereas the other died with multiorgan failure...
July 20, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28731915/cross-cultural-adaptation-of-the-self-care-of-hypertension-inventory-into-brazilian-portuguese
#10
Luana Claudia Jacoby Silveira, Eneida Rejane Rabelo-Silva, Christiane Whast Ávila, Leila Beltrami, Victoria Vaughan Dickson, Barbara Riegel
BACKGROUND: Lifestyle changes and treatment adherence still constitute a challenge to healthcare providers involved in the care of persons with hypertension. The lack of validated instruments measuring the ability of hypertensive patients to manage their disease has slowed research progress in this area. The Self-care of Hypertension Inventory, originally developed in the United States, consists of 23 items divided across 3 scales: Self-care Maintenance, Self-care Management, and Self-care Confidence...
July 20, 2017: Journal of Cardiovascular Nursing
https://www.readbyqxmd.com/read/28731901/should-first-line-empiric-treatment-strategies-for-neonates-cover-coagulase-negative-staphylococcal-infections-in-kenya
#11
Anna C Seale, Christina W Obiero, Kelsey Jones, Hellen C Barsosio, Johnstone Thitiri, Moses Ngari, Susan Morpeth, Shebe Mohammed, Greg Fegan, Neema Mturi, James A Berkley
BACKGROUND: Neonatal mortality remains high in sub-Saharan Africa, and a third of deaths are estimated to result from infection. Whilst coagulase negative staphylococci (CoNS) are leading neonatal pathogens in resource-rich settings, their role, and the need for early anti-staphylococcal treatment in empiric antibiotic guidelines, is unknown in sub-Saharan Africa. METHODS: We examined systematic clinical and microbiologic surveillance data from all neonatal admissions to Kilifi County Hospital (1998-2013) to determine associated case fatality and/or prolonged duration of admission associated with CoNS in neonates treated according to standard WHO guidelines...
July 18, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28731846/presence-and-future-of-culturing-bacteria
#12
Jörg Overmann, Birte Abt, Johannes Sikorski
The cultivation of bacteria is highly biased toward a few phylogenetic groups. Many of the currently underexplored bacterial lineages likely have novel biosynthetic pathways and unknown biochemical features. New cultivation concepts have been developed based on an improved understanding of the ecology of previously not-cultured bacteria. Particularly successful were improved media that mimic the natural types and concentrations of substrates and nutrients, high-throughput cultivation techniques, and approaches that exploit biofilm formation and bacterial interactions...
July 21, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28731824/evolutionary-scenarios-and-primate-natural-history
#13
Harry W Greene
Scenarios summarize evolutionary patterns and processes by interpreting organismal traits and their natural history correlates in a phylogenetic context. They are constructed by (1) describing phenotypes (including physiology and behavior), ideally with attention to formative roles of development, experience, and culture; (2) inferring homologies, homoplasies, ancestral character states, and their transformations with phylogenetic analyses; and (3) integrating those components with ecological and other ancillary data...
August 2017: American Naturalist
https://www.readbyqxmd.com/read/28731692/quinolinyl-nitrone-rp19-induces-neuroprotection-after-transient-brain-ischemia
#14
Maria Irene Ayuso, Emma Martinez-Alonso, Mourad Chioua, Alejandro Escobar-Peso, Rafael Gonzalo-Gobernado, Joan Montaner, José Marco-Contelles, Alberto Alcazar
There is a need to develop additional effective therapies for ischemic stroke. Nitrones, which were first developed as reactive oxygen species (ROS)-trapping compounds, have been proposed as neuroprotective agents for ischemic stroke, a ROS-related disorder. The previous reported ROS-trapping compound, quinolyl nitrone RP19, is here being assayed to induce neuroprotection to ischemia-reperfusion injury in three experimental ischemia models: (i), oxygen-glucose deprivation (OGD) on primary neuronal cultures; (ii), transient global cerebral ischemia in four-vessel occlusion model; and (iii), transient focal cerebral ischemia in middle cerebral artery occlusion (tMCAO) model...
July 21, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28731670/calcaneus-osteomyelitis-secondary-to-guthrie-test-case-report
#15
Tugce Tural Kara, Tugba Erat, Halil Ozdemir, Aysun Yahsi, Suat Fitoz, Ergin Ciftci, Erdal Incea
Calcaneus osteomyelitis is a rare infection in newborns. Invasive procedures, prematurity and low birth wight are some causative factors. The clinical signs may be mild. We report a neonate with calcaneous osteomyelitis which was secondary to a Guthrie test sample. She was admitted to hospital with swelling, redness and increased temperature on her heel. Superficial tissue ultrasound showed fluid collection with heavy content was observed on the posterior of the right foot. Puncture and drainage was performed and methicillin-susceptible Staphylococcus aureus was isolated from the drainage culture...
August 1, 2017: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/28731660/-excessive-crying-in-infants-in-the-first-months-of-life-review
#16
REVIEW
Patricio Kenny
Cry-fuss problems are among the most common clinical presentations in the first few months of life. Cry-fuss behaviour emerges out of a complex interplay of cultural, psychosocial, environmental and biologic factors, with organic disturbance implicated in only 5% of cases. A holistic biopsychosocial approach can be most effective. This article reviews recent evidence to update clinical management, and will attempt to reconceptualize infant colic definition.
August 1, 2017: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/28731643/transcriptome-based-identification-of-the-optimal-reference-cho-genes-for-normalisation-of-qpcr-data
#17
Adam J Brown, Suzanne Gibson, Diane Hatton, David C James
Real-time quantitative PCR (qPCR) is the standard method for determination of relative changes in mRNA transcript abundance. Analytical accuracy, precision, and reliability are critically dependent on the selection of internal control reference genes. In this study we have identified optimal reference genes that can be utilized universally for qPCR analysis of CHO cell mRNAs. Initially, transcriptomic datasets were analysed to identify eight endogenous genes that exhibited high expression stability across four distinct CHO cell lines sampled in different culture phases...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731639/exploring-family-home-food-environments-household-resources-needed-to-utilise-weekly-deliveries-of-free-fruits-and-vegetables
#18
Sophie A Carty, Louise A Mainvil, John D Coveney
AIM: An adapted ethnographic approach was used to explore household factors that influence family fruit and vegetable consumption when access and cost barriers are removed. 'Structural' barriers, such as food affordability and accessibility, are likely to influence fruit and vegetable consumption in disadvantaged households, but households may require additional resources (human and social) to increase consumption. METHODS: Five low-income and five high-income households with children (N = 39 individuals) were observed in their home environment for three months...
April 2017: Nutrition & Dietetics: the Journal of the Dietitians Association of Australia
https://www.readbyqxmd.com/read/28731631/substrate-curvature-restricts-spreading-and-induces-differentiation-of-human-mesenchymal-stem-cells
#19
Sang Joo Lee, Shengyuan Yang
While cells attach, spread, migrate, proliferate, and differentiate in three-dimensional (3D) micromechanical environments, the mechanical factors of these environments influence the shapes, sizes, and adhesion forces of the cells. Here, we culture human mesenchymal stem cells (hMSCs) on a unique class of curvature-defined substrates, micro glass ball embedded polyacrylamide gels, prepared with an improved protocol, and we investigate the spreading responses of the hMSCs on the glass balls to study the effects of substrate curvature on the spreading of hMSCs...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731612/mesoderm-lineage-3d-tissue-constructs-are-produced-at-large-scale-in-a-3d-stem-cell-bioprocess
#20
Jae Min Cha, Athanasios Mantalaris, Sunyoung Jung, Yurim Ji, Oh Young Bang, Hojae Bae
Various studies have presented different approaches to direct pluripotent stem cell differentiation such as applying defined sets of exogenous biochemical signals and genetic/epigenetic modifications. Although differentiation to target lineages was successfully regulated, such conventional methods are often complicated, laborious, and not cost-effective to be employed to the large-scale production of 3D stem cell-based tissue constructs. In this study, we developed a 3D-culture platform that could realize the large-scale production of mesoderm lineage tissue constructs from embryonic stem cells (ESCs)...
July 21, 2017: Biotechnology Journal
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