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Fat derived stem cell

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https://www.readbyqxmd.com/read/28713978/bone-marrow-mesenchymal-stem-cell%C3%A2-derived-extracellular-vesicles-improve-the-survival-of-transplanted-fat-grafts
#1
He Huang, Shaoqing Feng, Wenjie Zhang, Wei Li, Peng Xu, Xiangsheng Wang, Ai Ai
Autologous fat grafting is a promising surgical technique for soft tissue augmentation, reconstruction and rejuvenation. However, it is limited by the low survival rate of the transplanted fat, due to the slow revascularization of such grafts. Previous studies have demonstrated that bone marrow mesenchymal stem cell‑derived extracellular vesicles (BMSC‑EVs) are proangiogenic. The present study aimed to investigate whether BMSC‑EVs could improve the survival of transplanted fat grafts. Extracellular vesicles were isolated from the supernatant of cultured rat bone marrow mesenchymal stem cells, and characterized by flow cytometry and scanning electron microscopy...
July 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713821/comparative-analysis-of-the-cell-fates-of-induced-schwann-cells-from-subcutaneous-fat-tissue-and-na%C3%A3-ve-schwann-cells-in-the-sciatic-nerve-injury-model
#2
Mingzi Zhang, Mei Hua Jiang, Dae-Wook Kim, Woosung Ahn, Eunkyung Chung, Youngsook Son, Guangfan Chi
PURPOSE: The fate and function of the induced Schwann cells (iSCs) like cells from adipose tissue have not been critically evaluated in vivo after transplantation. The objective of this study is to compare the fate of iSCs with naïve SCs (nSCs) after transplantation into the lesion sites of sciatic nerve, respectively. METHODS: Adipose-derived stem cells from eGFP-expressing transgenic rat's subcutaneous fat were induced to iSCs in vitro. iSCs were injected to the sciatic nerve lesion area after crush injury and the cells fate was comparatively analyzed with that of nSCs from the same rat...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28698402/granulocyte-colony-stimulating-factor-treatment-in-non-alcoholic-fatty-liver-disease-beyond-marrow-cell-mobilization
#3
Ho Hyun Nam, Dae Won Jun, Kiseok Jang, Waqar Khalid Saeed, Jai Sun Lee, Hyeon Tae Kang, Yeon Ji Chae
Protective effects of granulocyte colony stimulating factor (G-CSF) in acute liver injury via marrow cell mobilization have been reported in several studies. But exact mode of action and optimal protocol of G-CSF has been still doubt in chronic disease. Here we investigated mode of action and optimization of G-CSF as a treatment for non-alcoholic fatty liver disease (NAFLD). Various doses of conventional G-CSF (30 μg/kg once weekly, once daily for 5 days, twice weekly) and long acting G-CSF (30 μg/kg once a month) were evaluated in two kinds of NAFLD animal models to optimize the G-CSF protocol...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28693305/comparison-the-characteristics-of-canine-adipose-tissue-derived-mesenchymal-stem-cells-extracted-from-different-sites-and-at-different-passage-numbers
#4
Kevin M Yaneselli, Cristiana Palma Kuhl, Paula Barros Terraciano, Fernanda de Los Santos de Oliveira, Sabrina Beal Pizzato, Kamila Pazza, Alessandra Bileski Magrisso, Vanessa Torman, Analía Rial, María Moreno, Silvia Llambí, Elizabeth Cirne Lima, Jacqueline Maisonnave
Mesenchymal Stem Cells (MSCs) have attractive characteristics for therapy in animal models and veterinary medicine, due to the capacity of inducing tissue regeneration and immunomodulation. The objective of this study was to evaluate the differences between canine adipose tissue-derived MSCs (AD-MSCs) extracted from subcutaneous (Sc) and visceral (Vs) sites. Surface antigenic markers, in vitro differentiation and mineralized matrix quantification of AD-MSCs in different passages (P4, P6 and P8) were studied...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28672891/osteogenic-potency-of-dedifferentiated-fat-cells-isolated-from-elderly-people-with-osteoporosis
#5
Wei Hao, Chuanqiang Jiang, Ming Jiang, Tian Wang, Xin Wang
Mature adipocytes are the major cell type in adipose tissue. This study aimed to explore the osteogenic potency of dedifferentiated fat cells obtained from osteoporotic patients (opDFATs) in vitro and in vivo. Mature adipocytes and adipose-derived stem cells (opASCs) were harvested from subcutaneous adipose tissue. Mature adipocytes were dedifferentiated to produce opDFATs by the ceiling culture method. OpDFATs were osteogenically induced in vitro with opASCs as a control. Cell growth, alkaline phosphatase (ALPase) activity and cell mineralization were determined, and expression levels of osteogenesis-specific genes (collagen I, osteocalcin and bone sialoprotein) were analyzed using quantitative reverse transcription polymerase chain reaction...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28653440/treatment-of-breast-cancer-related-lymphedema-with-adipose-derived-regenerative-cells-and-fat-grafts-a-feasibility-and-safety-study
#6
Navid Mohamadpour Toyserkani, Charlotte Harken Jensen, Ditte Caroline Andersen, Søren Paludan Sheikh, Jens Ahm Sørensen
Breast cancer-related lymphedema (BCRL) is a debilitating late complication with a lack of treatment opportunities. Recent studies have suggested that mesenchymal stromal cells can alleviate lymphedema. Herein, we report the results from the first human pilot study with freshly isolated adipose-derived regenerative cells (ADRC) for treating lymphedema with 6 months follow-up. Ten BCRL patients were included. ADRC was injected directly into the axillary region, which was combined with a scar-releasing fat graft procedure...
June 27, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28640866/diet-induced-adipose-tissue-expansion-is-mitigated-in-mice-with-a-targeted-inactivation-of-mesoderm-specific-transcript-mest
#7
Rea P Anunciado-Koza, Justin Manuel, Randall L Mynatt, Jingying Zhang, Leslie P Kozak, Robert A Koza
Interindividual variation of white adipose tissue (WAT) expression of mesoderm specific transcript (Mest), a paternally-expressed imprinted gene belonging to the α/β-hydrolase fold protein family, becomes apparent among genetically inbred mice fed high fat diet (HFD) and is positively associated with adipose tissue expansion (ATE). To elucidate a role for MEST in ATE, mice were developed with global and adipose tissue inactivation of Mest. Mice with homozygous (MestgKO) and paternal allelic (MestpKO) inactivation of Mest were born at expected Mendelian frequencies, showed no behavioral or physical abnormalities, and did not perturb expression of the Mest locus-derived microRNA miR-335...
2017: PloS One
https://www.readbyqxmd.com/read/28614775/changes-in-viability-of-rat-adipose-derived-stem-cells-isolated-from-abdominal-perinuclear-adipose-tissue-stimulated-with-pulsed-electromagnetic-field
#8
A Baranowska, B Skowron, B Nowak, K Ciesielczyk, P Guzdek, K Gil, J Kaszuba-Zwoinska
Previous experiments demonstrated that low-frequency electromagnetic field (LF-EMF) may activate cellular death pathways in proliferating cells. Therefore, we hypothesized that LF-EMF may also influence viability of highly proliferating undifferentiated adipose-derived stem cells. Obesity is classified as a civilization disease; its etiopathogenesis is presumed to include both genetic predisposition and influence of modified environmental factors, such as unbalanced diet with excess calories and/or too low physical activity...
April 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28605392/transfer-of-mammary-gland-forming-ability-between-mammary-basal-epithelial-cells-and-mammary-luminal-cells-via-extracellular-vesicles-exosomes
#9
Meng-Chieh Lin, Shih-Yin Chen, Pei-Lin He, Wen-Ting Luo, Hua-Jung Li
Cells can communicate via exosomes, ~100-nm extracellular vesicles (EVs) that contain proteins, lipids, and nucleic acids. Non-adherent/mesenchymal mammary epithelial cell (NAMEC)-derived extracellular vesicles can be isolated from NAMEC medium via differential ultracentrifugation. Based on their density, EVs can be purified via ultracentrifugation at 110,000 x g. The EV preparation from ultracentrifugation can be further separated using a continuous density gradient to prevent contamination with soluble proteins...
June 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28597066/effects-of-fat-grafting-containing-stem-cells-in-microstomia-and-microcheilia-derived-from-systemic-sclerosis
#10
Oskar Blezien, Francesco D'Andrea, Giovanni Francesco Nicoletti, Giuseppe Andrea Ferraro
BACKGROUND: Systemic sclerosis (SSc) is an autoimmune disease with multisystem involvement, dominated by a general fibrosis. The early stage of the disease is associated with progressive damage to microcirculation, particularly in the respiratory tract, the gastrointestinal tract and skin. The face assumes a typical appearance characterized by microstomia (reduction of mouth opening) and microcheilia (thinning of the lips). These conditions cause a considerable reduction in performance status of patients...
June 8, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28595579/omental-adipose-tissue-is-a-more-suitable-source-of-canine-mesenchymal-stem-cells
#11
Francisca Bahamondes, Estefania Flores, Gino Cattaneo, Flavia Bruna, Paulette Conget
BACKGROUND: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue...
June 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28587666/protocol-for-a-systematic-review-of-the-efficacy-of-fat-grafting-and-platelet-rich-plasma-for-wound-healing
#12
Oliver J Smith, Muholan Kanapathy, Ankur Khajuria, Max Prokopenko, Nadine Hachach-Haram, Haroon Mann, Ash Mosahebi
BACKGROUND: The use of fat grafting as a reconstructive surgical option is becoming much more common. Adipose-derived stem cells found in fat grafts are believed to facilitate wound healing via differentiation into fibroblasts and keratinocytes and the release of pro-healing growth factors. Several small studies have shown a positive effect of fat grafting in healing of wounds of a variety of aetiologies. When fat is combined with autologous platelet-rich plasma (PRP), there may be enhanced healing effects...
June 6, 2017: Systematic Reviews
https://www.readbyqxmd.com/read/28586071/a-comparative-study-of-the-influence-of-two-types-of-phema-stents-on-the-differentiation-of-ascs-to-myocardial-cells
#13
Shen Lao, Jing Xu, Yunqi Liu, Songwang Cai, Lin Lin, Junhang Zhang, Dongmei Cai, Shengli Yin
In the present study, subcutaneous fat was obtained from adult women that had undergone conventional liposuction surgery. A comparative study was performed to investigate the effect of transparent and white poly‑β‑hydroxyethyl methacrylate (PHEMA) stents, which have different surface and cross‑sectional morphological characteristics, on the differentiation of adipose‑derived stem cells (ASCs) into myocardial cells. The cell counting kit‑8 assay revealed that cell growth increased at varying rates among the different treatment groups...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28580233/connecting-bone-and-fat-the-potential-role-for-sclerostin
#14
Heather Fairfield, Clifford J Rosen, Michaela R Reagan
Sclerostin (SOST), a protein secreted from mature osteocytes in response to mechanical unloading and other stimuli, inhibits the osteogenic Wnt/β-catenin pathway in mesenchymal stem cells (MSCs) impeding their ability to differentiate into mineralizing osteoblasts. PURPOSE: This review summarizes the crosstalk between adipose tissue and bone. It also reviews the origin, regulation, and role of SOST in osteogenesis and brings attention to an emerging role of this protein in the regulation of adipogenesis...
June 2017: Current Molecular Biology Reports
https://www.readbyqxmd.com/read/28576462/platelet-rich-plasma-enriched-autologous-fat-graft-in-regenerative-and-aesthetic-facial-surgery-technical-note
#15
F Picard, B Hersant, S La Padula, J-P Meningaud
The goal of adding platelet-rich plasma (PRP) to autologous fat graft is to increase the survival rate of the graft. After their activation, platelets release some important growth factors. As a result, PRP may increase the proliferation and differentiation of Adipose-derived stem cells (ASCs) into adipocytes, improve fat graft vascularisation, and may block the apoptosis of grafted adipocytes. The other benefit expected from the addition of PRP to fat graft is the improvement of cutaneous trophicity above the grafted areas...
May 30, 2017: Journal of Stomatology, Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28573141/c-kit-positive-adipose-tissue-derived-mesenchymal-stem-cells-promote-the-growth-and-angiogenesis-of-breast-cancer
#16
Wenjie Li, Haiqian Xu, Cheng Qian
BACKGROUND: Adipose tissue-derived mesenchymal stem cells (ASCs) improve the regenerative ability and retention of fat grafts for breast reconstruction in cancer patients following mastectomy. However, ASCs have also been shown to promote breast cancer cell growth and metastasis. For the safety of ASC application, we aimed to identify specific markers for the subpopulation of ASCs that enhance the growth of breast cancer. METHODS: ASCs and bone marrow-derived vascular endothelial progenitor cells (EPCs) were isolated from Balb/c mice...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28571817/development-of-orbital-adipose-derived-stem-cells-as-a-model-for-studying-the-formation-of-baggy-lower-eyelids
#17
Xi Chen, Guangpeng Liu
In old population, there is often a protrusion of the orbital fat pad underlying the skin in the lower eyelids, giving an aspect of palpable pouches. It is generally thought that orbital fat hyperplasia is the main contributing factor to the formation of baggy lower eyelids, and resection of excessive orbital fat pad is routinely performed during the eyelid cosmetic surgery. In our clinical study, however, it was revealed that the adipocytes in orbital fat tissue from older people became smaller compared to those from the young individuals...
June 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28567229/platelet-derived-growth-factor-receptor-positive-pericytic-cells-of-white-adipose-tissue-from-critical-limb-ischemia-patients-display-mesenchymal-stem-cell-like-properties
#18
Eo Jin Kim, Sang Gyo Seo, Hyuk Soo Shin, Doo Jae Lee, Ji Hye Kim, Dong Yeon Lee
BACKGROUND: The pericytes in the blood vessel wall have recently been identified to be important in regulating vascular formation, stabilization, remodeling, and function. We isolated and identified pericyte-like platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells from the stromal vascular fraction (SVF) of adipose tissue from critical limb ischemia (CLI) patients and investigated their potential as a reliable source of stem cells for cell-based therapy. METHODS: De-identified subcutaneous fat tissues were harvested after amputation in CLI patients...
June 2017: Clinics in Orthopedic Surgery
https://www.readbyqxmd.com/read/28526415/lnk-deficiency-aggravates-palmitate-induced-preadipocyte-apoptosis
#19
Jie-Yi Du, Chen-Chen Jin, Guo-Hao Wang, Xiong-Qing Huang, Jian-Ding Cheng, Xue-Jun Wen, Xiao-Miao Zhao, Guan-Lei Wang
LNK (SH2B3) is an intracellular adaptor protein that negatively regulates cellular proliferation or self-renewal of hematopoietic stem cells and some other progenitor cells. LNK is also recognized as a key regulator of insulin resistance and inflammatory responses in several tissues and organs. The function of LNK in adipose tissue is unknown. We previously demonstrated that type 2 diabetes mellitus (T2DM) mouse model had elevated serum free fatty acids (FFAs) levels and increased preadipocyte apoptosis in visceral fat tissue, showing the occurrence of lipotoxicity...
August 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28526089/adipose-derived-mesenchymal-stem-cells-from-liposuction-and-resected-fat-are-feasible-sources-for-regenerative-medicine
#20
Sandra Schneider, Marina Unger, Martijn van Griensven, Elizabeth R Balmayor
BACKGROUND: The use of mesenchymal stem cells (MSCs) in research and in regenerative medicine has progressed. Bone marrow as a source has drawbacks because of subsequent morbidities. An easily accessible and valuable source is adipose tissue. This type of tissue contains a high number of MSCs, and obtaining higher quantities of tissue is more feasible. Fat tissue can be harvested using different methods such as liposuction and resection. First, a detailed isolation protocol with complete characterization is described...
May 19, 2017: European Journal of Medical Research
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