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Fat derived stem cell

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https://www.readbyqxmd.com/read/28217409/muscle-derived-stem-cells-stimulate-muscle-myofiber-repair-and-counteract-fat-infiltration-in-a-diabetic-mouse-model-of-critical-limb-ischemia
#1
J Tsao, I Kovanecz, N Awadalla, R Gelfand, I Sinha-Hikim, R A White, N F Gonzalez-Cadavid
BACKGROUND: Critical Limb Ischemia (CLI) affects patients with Type 2 Diabetes (T2D) and obesity, with high risk of amputation and post-surgical mortality, and no effective medical treatment. Stem cell therapy, mainly with bone marrow mesenchymal, adipose derived, endothelial, hematopoietic, and umbilical cord stem cells, is promising in CLI mouse and rat models and is in clinical trials. Their general focus is on angiogenic repair, with no reports on the alleviation of necrosis, lipofibrosis, and myofiber regeneration in the ischemic muscle, or the use of Muscle Derived Stem Cells (MDSC) alone or in combination with pharmacological adjuvants, in the context of CLI in T2D...
December 2016: Journal of Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28212548/branched-chain-amino-acids-prevent-hepatic-fibrosis-and-development-of-hepatocellular-carcinoma-in-a-non-alcoholic-steatohepatitis-mouse-model
#2
Kai Takegoshi, Masao Honda, Hikari Okada, Riuta Takabatake, Naoto Matsuzawa-Nagata, Jean S Campbell, Masashi Nishikawa, Tetsuro Shimakami, Takayoshi Shirasaki, Yoshio Sakai, Taro Yamashita, Toshinari Takamura, Takuji Tanaka, Shuichi Kaneko
Oral supplementation with branched-chain amino acids (BCAA; leucine, isoleucine, and valine) in patients with liver cirrhosis potentially suppresses the incidence of hepatocellular carcinoma (HCC) and improves event-free survival. However, the detailed mechanisms of BCAA action have not been fully elucidated. BCAA were administered to atherogenic and high-fat (Ath+HF) diet-induced nonalcoholic steatohepatitis (NASH) model mice. Liver histology, tumor incidence, and gene expression profiles were evaluated. Ath+HF diet mice developed hepatic tumors at a high frequency at 68 weeks...
February 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28210976/regenerative-medicine-for-parkinson-s-disease-using-differentiated-nerve-cells-derived-from-human-buccal-fat-pad-stem-cells
#3
Haruka Takahashi, Hiroshi Ishikawa, Akira Tanaka
The purpose of this study was to evaluate the utility of human adipose stem cells derived from the buccal fat pad (hBFP-ASCs) for nerve regeneration. Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive death of dopaminergic neurons. PD is a candidate disease for cell replacement therapy because it has no fundamental therapeutic methods. We examined the properties of neural-related cells induced from hBFP-ASCs as a cell source for PD treatment. hBFP-ASCs were cultured in neurogenic differentiation medium for about 2 weeks...
February 16, 2017: Human Cell
https://www.readbyqxmd.com/read/28198109/progenitors-in-peripheral-nerves-launch-heterotopic-ossification
#4
Elizabeth A Olmsted-Davis, Elizabeth A Salisbury, Diana Hoang, Eleanor L Davis, ZaWaunyka Lazard, Corinne Sonnet, Thomas A Davis, Jonathan A Forsberg, Alan R Davis
Studies presented here, using a murine model of bone morphogenetic protein type 2 (BMP2)-induced heterotopic ossification (HO) show that the protein initiates HO by signaling through progenitors in the endoneurium of peripheral nerves. In the mouse, these cells were identified in the endoneurium one day after BMP2 induction using antibody against phosphoSMAD (PS) 1, 5, and 8. Studies conducted in a tracking mouse that contains a tamoxifen-regulated Wnt1-Cre recombinase crossed with a td Tomato red (TR) reporter (Wnt1(CreErt) :Ai9Tm) confirmed their neural origin...
February 15, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28186685/concise-review-fat-and-furious-harnessing-the-full-potential-of-adipose-derived-stromal-vascular-fraction
#5
Jordan A Dykstra, Tiffany Facile, Ryan J Patrick, Kevin R Francis, Samuel Milanovich, Jill M Weimer, Daniel J Kota
Due to their capacity to self-renew, proliferate and generate multi-lineage cells, adult-derived stem cells offer great potential for use in regenerative therapies to stop and/or reverse degenerative diseases such as diabetes, heart failure, Alzheimer's disease and others. However, these subsets of cells can be isolated from different niches, each with differing potential for therapeutic applications. The stromal vascular fraction (SVF), a stem cell enriched and adipose-derived cell population, has garnered interest as a therapeutic in regenerative medicine due to its ability to secrete paracrine factors that accelerate endogenous repair, ease of accessibility and lack of identified major adverse effects...
January 6, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28175497/216%C3%A2-human-fat-derived-mesenchymal-stem-cells-bioengineered-to-secrete-bmp4-are-nononcogenic-suppress-glioma-and-prolong-survival
#6
Sara Ganaha, Rawan Al-Kharboosh, Alejandro Ruiz-Valls, Hugo Guerrero-Cazares, Alfredo Quinones-Hinojosa
No abstract text is available yet for this article.
August 1, 2016: Neurosurgery
https://www.readbyqxmd.com/read/28173811/marrow-adipocytes-inhibit-the-differentiation-of-mesenchymal-stem-cells-into-osteoblasts-via-suppressing-bmp-signaling
#7
Basem M Abdallah
BACKGROUND: Reduced bone formation is associated with increased bone marrow fat in many bone-loss related diseases including aging, post-menopause, and anorexia nervosa. Several lines of evidence suggested the regulation of osteogenesis and adipogenesis of the bone marrow-derived mesenchymal (skeletal) stem cells (BMSCs) by paracrine mediators. This study aimed to investigate the impact of adipocytes-secreted factors on the cell proliferation and osteoblast differentiation of BMSCs. METHODS: Serum free conditioned medium (CM-Adipo) was collected from stromal ST2 cells-derived adipocytes...
February 7, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28170170/the-infrapatellar-fat-pad-as-a-source-of-perivascular-stem-cells-with-increased-chondrogenic-potential-for-regenerative-medicine
#8
Paul Hindle, Nusrat Khan, Leela Biant, Bruno Péault
Perivascular stem cells (PSCs) are the natural ancestors of mesenchymal stem cells (MSCs) and are the stem cells responsible for homeostasis and repair in vivo. Prospectively identified and isolated PSCs have demonstrated increased plasticity and osteogenic potential. Cells from the infrapatellar fat pad (IFP) have demonstrated increased chondrogenic potential compared with those from subcutaneous fat. This research assessed the chondrogenic potential of IFP PSCs compared with MSCs from the IFP and bone marrow...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28159868/using-murine-derived-primary-intestinal-enteroids-for-studies-of-dietary-triglyceride-absorption-and-lipoprotein-synthesis-and-to-determine-the-role-of-intestine-specific-apoc-iii
#9
Javeed J Jattan, Cayla N Rodia, Diana Li, Adama C Diakhate, Hongli Dong, Amy M Bataille, Noah F Shroyer, Alison B Kohan
Since its initial report in 2009, the intestinal enteroid culture system has been a powerful tool used to study stem cell biology and development in the gastrointestinal tract. However, a major question is whether enteroids retain intestinal function and physiology. There have been significant contributions describing ion transport physiology of human intestinal organoid cultures, as well as physiology of gastric organoids, but critical studies on dietary fat absorption and chylomicron synthesis in primary intestinal enteroids have not been undertaken...
February 3, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28156060/the-power-of-fat-and-its-adipose-derived-stromal-cells-emerging-concepts-for-fibrotic-scar-treatment
#10
REVIEW
Maroesjka Spiekman, Joris A van Dongen, Joep C Willemsen, Delia L Hoppe, Berend van der Lei, Martin C Harmsen
Lipofilling or lipografting is a novel and promising treatment method for reduction or prevention of dermal scars after injury. Ample anecdotal evidence from case reports supports the scar-reducing properties of adipose tissue grafts. However, only a few properly controlled and designed clinical trials have been conducted thus far on this topic. Also, the underlying mechanism by which lipofilling improves scar aspect and reduces neuropathic scar pain remains largely undiscovered. Adipose-derived stromal or stem cells (ADSC) are often described to be responsible for this therapeutic effect of lipofilling...
February 3, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28155209/do-mesenchymal-stem-cells-derived-from-atypical-lipomatous-tumors-have-greater-differentiation-potency-than-cells-from-normal-adipose-tissues
#11
Hiroyuki Inatani, Norio Yamamoto, Katsuhiro Hayashi, Hiroaki Kimura, Akihiko Takeuchi, Shinji Miwa, Takashi Higuchi, Kensaku Abe, Yuta Taniguchi, Satoshi Yamada, Kiyofumi Asai, Takanobu Otsuka, Hiroyuki Tsuchiya
BACKGROUND: The p53 protein in mesenchymal stem cells (MSCs) regulates differentiation to osteogenic or adipogenic lineage. Because p53 function is depressed in most malignancies, if MSCs in malignancy also have p53 hypofunction, differentiation therapy to osteogenic or adipogenic lineage may be an effective treatment. We therefore wished to begin to explore this idea by evaluating atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) cells, because murine double minute 2 (MDM2) gene amplification, which leads to p53 hypofunction, is found in almost all ALT/WDLs...
February 2, 2017: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/28128224/pyrintegrin-induces-soft-tissue-formation-by-transplanted-or-endogenous-cells
#12
Bhranti S Shah, Mo Chen, Takahiro Suzuki, Mildred Embree, Kimi Kong, Chang H Lee, Ling He, Lusai Xiang, Jeffrey A Ahn, Sheng Ding, Jeremy J Mao
Focal adipose deficiency, such as lipoatrophy, lumpectomy or facial trauma, is a formidable challenge in reconstructive medicine, and yet scarcely investigated in experimental studies. Here, we report that Pyrintegrin (Ptn), a 2,4-disubstituted pyrimidine known to promote embryonic stem cells survival, is robustly adipogenic and induces postnatal adipose tissue formation in vivo of transplanted adipose stem/progenitor cells (ASCs) and recruited endogenous cells. In vitro, Ptn stimulated human adipose tissue derived ASCs to differentiate into lipid-laden adipocytes by upregulating peroxisome proliferator-activated receptor (PPARγ) and CCAAT/enhancer-binding protein-α (C/EBPα), with differentiated cells increasingly secreting adiponectin, leptin, glycerol and total triglycerides...
January 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28115038/identification-of-mfge8-in-mesenchymal-stem-cell-secretome-as-an-anti-fibrotic-factor-in-liver-fibrosis
#13
Yu Jin Jang, Su Yeon An, Jong-Hoon Kim
The beneficial paracrine roles of mesenchymal stem cells (MSCs) in tissue repair have potential in therapeutic strategies against various diseases. However, the key therapeutic factors secreted from MSCs and their exact molecular mechanisms of action remain unclear. In this study, the cell-free secretome of umbilical cord-derived MSCs showed significant anti-fibrotic activity in the mouse models of liver fibrosis. The involved action mechanism was the regulation of hepatic stellate cell activation by direct inhibition of the TGFβ/Smad-signaling...
February 2017: BMB Reports
https://www.readbyqxmd.com/read/28112572/rna-expression-profiling-reveal-differentially-regulated-growth-factor-and-receptor-expression-in-redirected-cancer-cells
#14
Hannah S Schmucker, Jang Pyo Park, Marie-May Coissieux, Mohamed Bentires-Alj, F Alex Feltus, Brian Booth
Tumorigenic cells can be redirected to adopt a normal phenotype when transplanted into cleared mammary fat pads of juvenile female mice in specific ratios with normal epithelial cells. The redirected tumorigenic cells enter stem cell niches and provide progeny that differentiate into all mammary epithelial subtypes. We have developed an in vitro model that mimics the in vivo phenomenon. The shift in phenotype to redirection should be accomplished through a return to a normal gene expression state. To measure this shift we interrogated the transcriptome of various in vitro model states in search for casual genes...
January 23, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28109150/-isolation-culture-and-identification-of-adipose-derived-stem-cells-from-sd-rat-adipose-tissues-subjected-to-long-term-cryopreservation
#15
Qin Liu, Liping Wang, Fang Chen, Yi Zhang
Objective To study the feasibility of isolation and culture of adipose-derived stem cells (ADSCs) from SD rat adipose tissues subjected to long-term cryopreservation. Methods We took inguinal fat pads from healthy SD rats. Adipose tissues were stored with 100 mL/L dimethyl sulfoxide (DMSO) combined with 900 mL/L fetal bovine serum (FBS) in liquid nitrogen. Three months later, the adipose tissues were resuscitated for the isolation and culture of ADSCs. The growth status and morphology were observed. The growth curve and cell surface markers CD29, CD45, CD90 of the 3rd passage cells were analyzed respectively by CCK-8 assay and immunocytochemistry...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109149/-indirect-coculture-of-human-infrapatellar-fat-pad-derived-stem-cells-with-osteoarthritic-chondrocytes-induces-their-chondrogenesis
#16
Junjun Yang, Cheng Chen, Liu Yang, Xiongbo Song, Wenbin Xie, Shu Huang, Baorong Liu
Objective To promote phenotype recovery of osteoarthritic articular chondrocytes (OACs) and induce chondrogenic differentiation of infrapatellar fat pad-derived stem cells (IPFPSCs) by indirectly coculturing these two types of cells. Methods The OACs and IPFPSCs were isolated and cultured in vitro. This experiment included single IPFPSCs group, single OACs group, and coculture group. After cells were cultured in vitro with chondrogenic medium for 21 days, the chondrocyte phenotypes were determined by HE staining (cell morphology), Alcian blue staining (glycosaminoglycan content) and immunofluorescence cytochemistry (collagen 1, collagen 2, collagen 3, aggrecan, SOX9)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#17
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28106629/heat-shock-protein-70-overexpression-in-adipose-derived-stem-cells-enhances-fat-graft-survival
#18
Hao Feng, Lihong Qiu, Teng Zhang, Houyou Yu, Xianjie Ma, Yingjun Su, Hui Zheng, Yong Wang, Chenggang Yi
BACKGROUND: Autologous fat grafting is a prevalent technique used for soft-tissue augmentation; however, the poor survival rate of the grafted tissue remains a drawback of this method. Although adipose-derived stem cells (ASCs) are an attractive candidate for enhancing graft retention, the poor posttransplantation viability of these cells limits their application. Here we investigated whether overexpression of the antiapoptotic protein heat-shock protein 70 (Hsp70) could enhance ASCs' therapeutic potential for fat transplant survival...
January 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28102844/human-adipose-stem-cell-differentiation-is-highly-affected-by-cancer-cells-both-in-vitro-and-in-vivo-implication-for-autologous-fat-grafting
#19
Francesca Paino, Marcella La Noce, Diego Di Nucci, Giovanni Francesco Nicoletti, Rosa Salzillo, Alfredo De Rosa, Giuseppe Andrea Ferraro, Gianpaolo Papaccio, Vincenzo Desiderio, Virginia Tirino
Recent studies showed that mesenchymal stem cells derived from adipose tissue can promote tumour progression, raising some concerns regarding their use in regenerative medicine. In this context, we co-cultured either SAOS2 osteosarcoma or MCF7 breast cancer cells with human adipose stem cells (hASCs), in order to evaluate potential effects of cancer cells on hASCs differentiation, in vitro and in vivo. In this study we observed that both SAOS2 and MCF7 cell lines induced an increase in hASCs proliferation, compared to hASCs alone, but, surprisingly, neither changes in the expression of CD90, CD29, CD324 and vimentin, nor variations in the Twist and Slug mRNAs were detectable...
January 19, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28090705/osteogenic-differentiation-potential-of-adipose-derived-stem-cells-from-ovariectomized-mice
#20
Lei Wang, Chenglong Huang, Qing Li, Xiaomei Xu, Lin Liu, Kui Huang, Xiaoxiao Cai, Jingang Xiao
OBJECTIVES: Osteoporosis (OP) is a systemic disease caused by imbalance between bone resorption and bone formation, commonly resulting from post-menopausal oestrogen deficiency. Although osteogenic differentiation potential of adipose-derived stem cells (ASCs) has been demonstrated, the effect of OP on osteogenic differentiation of ASCs remains unclear. Here, our work has been designed to compare proliferative capacity and osteogenic differentiation ability of ASCs obtained from osteoporotic mice and normal control mice...
January 16, 2017: Cell Proliferation
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