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Fat derived stem cell

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https://www.readbyqxmd.com/read/28102844/human-adipose-stem-cell-differentiation-is-highly-affected-by-cancer-cells-both-in-vitro-and-in-vivo-implication-for-autologous-fat-grafting
#1
Francesca Paino, Marcella La Noce, Diego Di Nucci, Giovanni Francesco Nicoletti, Rosa Salzillo, Alfredo De Rosa, Giuseppe Andrea Ferraro, Gianpaolo Papaccio, Vincenzo Desiderio, Virginia Tirino
Recent studies showed that mesenchymal stem cells derived from adipose tissue can promote tumour progression, raising some concerns regarding their use in regenerative medicine. In this context, we co-cultured either SAOS2 osteosarcoma or MCF7 breast cancer cells with human adipose stem cells (hASCs), in order to evaluate potential effects of cancer cells on hASCs differentiation, in vitro and in vivo. In this study we observed that both SAOS2 and MCF7 cell lines induced an increase in hASCs proliferation, compared to hASCs alone, but, surprisingly, neither changes in the expression of CD90, CD29, CD324 and vimentin, nor variations in the Twist and Slug mRNAs were detectable...
January 19, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28090705/osteogenic-differentiation-potential-of-adipose-derived-stem-cells-from-ovariectomized-mice
#2
Lei Wang, Chenglong Huang, Qing Li, Xiaomei Xu, Lin Liu, Kui Huang, Xiaoxiao Cai, Jingang Xiao
OBJECTIVES: Osteoporosis (OP) is a systemic disease caused by imbalance between bone resorption and bone formation, commonly resulting from post-menopausal oestrogen deficiency. Although osteogenic differentiation potential of adipose-derived stem cells (ASCs) has been demonstrated, the effect of OP on osteogenic differentiation of ASCs remains unclear. Here, our work has been designed to compare proliferative capacity and osteogenic differentiation ability of ASCs obtained from osteoporotic mice and normal control mice...
January 16, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28071738/purification-and-differentiation-of-human-adipose-derived-stem-cells-by-membrane-filtration-and-membrane-migration-methods
#3
Hong Reng Lin, Chao-Wen Heish, Cheng-Hui Liu, Saradaprasan Muduli, Hsing-Fen Li, Akon Higuchi, S Suresh Kumar, Abdullah A Alarfaj, Murugan A Munusamy, Shih-Tien Hsu, Da-Chung Chen, Giovanni Benelli, Kadarkarai Murugan, Nai-Chen Cheng, Han-Chow Wang, Gwo-Jang Wu
Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28071703/mammary-extracellular-matrix-directs-differentiation-of-testicular-and-embryonic-stem-cells-to-form-functional-mammary-glands-in-vivo
#4
Robert D Bruno, Jodie M Fleming, Andrea L George, Corinne A Boulanger, Pepper Schedin, Gilbert H Smith
Previously, we demonstrated the ability of the normal mammary microenvironment (niche) to direct non-mammary cells including testicular and embryonic stem cells (ESCs) to adopt a mammary epithelial cell (MEC) fate. These studies relied upon the interaction of transplanted normal MECs with non-mammary cells within the mammary fat-pads of recipient mice that had their endogenous epithelium removed. Here, we tested whether acellular mammary extracellular matrix (mECM) preparations are sufficient to direct differentiation of testicular-derived cells and ESCs to form functional mammary epithelial trees in vivo...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28068868/mir-26a-mediates-adipogenesis-of-amniotic-fluid-mesenchymal-stem-stromal-cells-via-pten-cyclin-e1-and-cdk6
#5
Ourania Trohatou, Dimitra Zagoura, Nikos Orfanos, Kalliopi I Pappa, Evangelos Marinos, Nicholas Anagnou, Maria G Roubelakis
Recent findings indicate that microRNAs (miRNAs) are critical for the regulatory network of adipogenesis in human mesenchymal stem/stromal cells (MSCs). Fetal MSCs derived from amniotic fluid (AF-MSCs) represent a population of multipotent stem cells characterized by a wide range of differentiation properties that can be applied in cell-based therapies. In this study, miRNA microarray analysis was performed to assess miRNA expression in terminal differentiated AF-MSCs into adipocyte-like cells (AL cells). MiR-26a was identified in high expression levels in AL cells indicating a critical role in the process of adipogenesis...
January 9, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28027219/cancer-risk-after-fat-transfer-a-multicenter-case-cohort-study
#6
Terence M Myckatyn, I Janelle Wagner, Babak J Mehrara, Melissa A Crosby, Julie E Park, Bahjat F Qaqish, Dominic T Moore, Evan L Busch, Amanda K Silva, Surinder Kaur, David W Ollila, Clara N Lee
BACKGROUND: Fat transfer is an increasingly popular method for refining postmastectomy breast reconstructions. However, concern persists that fat transfer may promote disease recurrence. Adipocytes are derived from adipose-derived stem cells and express adipocytokines that can facilitate active breast cancer cells in laboratory models. The authors sought to evaluate the association between fat transfer to the reconstructed breast and cancer recurrence in patients diagnosed with local or regional invasive breast cancers...
January 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28024612/fabrication-of-a-three-dimensional-%C3%AE-tricalcium-phosphate-gelatin-containing-chitosan-based-nanoparticles-for-sustained-release-of-bone-morphogenetic-protein-2-implication-for-bone-tissue-engineering
#7
Farshid Bastami, Zahrasadat Paknejad, Maissa Jafari, Majid Salehi, Maryam Rezai Rad, Arash Khojasteh
Fabrication of an ideal scaffold having proper composition, physical structure and able to have sustained release of growth factors still is challenging for bone tissue engineering. Current study aimed to design an appropriate three-dimensional (3-D) scaffold with suitable physical characteristics, including proper compressive strength, degradation rate, porosity, and able to sustained release of bone morphogenetic protein-2 (BMP2), for bone tissue engineering. A highly porous 3-D β-tricalcium phosphate (β-TCP) scaffolds, inside of which two perpendicular canals were created, was fabricated using foam-casting technique...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28003721/primary-biliary-cholangitis-disease-pathogenesis-and-implications-for-established-and-novel-therapeutics
#8
REVIEW
Amitkumar Patel, Anil Seetharam
Primary Biliary Cholangitis is a progressive, autoimmune cholestatic liver disorder. Cholestasis with disease progression may lead to dyslipidemia, osteodystrophy and fat-soluble vitamin deficiency. Portal hypertension may develop prior to advanced stages of fibrosis. Untreated disease may lead to cirrhosis, hepatocellular cancer and need for orthotopic liver transplantation. Classically, diagnosis is made with elevation of alkaline phosphatase, demonstration of circulating antimitochondrial antibody, and if performed: asymmetric destruction/nonsupperative cholangitis of intralobular bile ducts on biopsy...
December 2016: Journal of Clinical and Experimental Hepatology
https://www.readbyqxmd.com/read/28002250/adipose-extracellular-matrix-stromal-vascular-fraction-gel-a-novel-adipose-tissue-derived-injectable-for-stem-cell-therapy
#9
Yao Yao, Ziqing Dong, Yunjun Liao, Pan Zhang, Jingjing Ma, Jianhua Gao, Feng Lu
BACKGROUND: Adipose-derived stem cells (ASCs) and other stromal vascular fraction (SVF) cells were used more often for stem cell therapy, even though limitations such as poor cell retention rate, complicated and expensive isolation processes, and the use of specific laboratory equipment need to be overcome. METHODS: Here, we developed a novel but simple method for generating an injectable mixture of SVF cells and native adipose extracellular matrix (ECM). It is a purely mechanical process in which lipoaspirate is processed into an "ECM/SVF-gel"...
December 19, 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28000114/evaluation-of-a-laminin-alginate-biomaterial-adipocytes-and-adipocyte-derived-stem-cells-interaction-in-animal-autologous-fat-grafting-model-using-7-tesla-magnetic-resonance-imaging
#10
Yo-Shen Chen, Yu-Sheng Hsueh, Yen-Yu Chen, Cheng-Yu Lo, Hao-Chih Tai, Feng-Huei Lin
Biomaterials are often added to autologous fat grafts both as supporting matrices for the grafted adipocytes and as cell carrier for adipose-derived stem cells (ADSCs). This in vivo study used an autologous fat graft model to test a lamininalginate biomaterial, adipocytes, and ADSCs in immune-competent rats. We transplanted different combinations of shredded autologous adipose tissue [designated "A" for adipose tissue]), laminin-alginate beads [designated "B" for bead], and ADSCs [designated "C" for cell]) into the backs of 15 Sprague-Dawley rats...
January 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27994952/retinoic-acid-is-abundantly-detected-in-different-depots-of-adipose-tissue-by-sers
#11
Jayakumar Perumal, Sandhya Sriram, Hann Qian Lim, Malini Olivo, Shigeki Sugii
Retinoic acid (RA) is essential for early developmental processes and stem cell differentiation, but less is known about its contributions to adult tissues and stem cells including adipose tissue. We previously demonstrated that many genes involved in RA synthesis and downstream pathway are differentially expressed in adipose-derived stem cells (ASCs) from visceral fat compared to those from subcutaneous fat, leading to changes in their early adipogenic functions. In order to study potential contributions of RA in adipose tissue, we measured tissue RA levels using a technique based on surface-enhanced Raman spectroscopy (SERS)...
October 2016: Adipocyte
https://www.readbyqxmd.com/read/27992452/enhancement-of-adipocyte-browning-by-angiotensin-ii-type-1-receptor-blockade
#12
Kana Tsukuda, Masaki Mogi, Jun Iwanami, Harumi Kanno, Hirotomo Nakaoka, Xiao-Li Wang, Hui-Yu Bai, Bao-Shuai Shan, Masayoshi Kukida, Akinori Higaki, Toshifumi Yamauchi, Li-Juan Min, Masatsugu Horiuchi
Browning of white adipose tissue (WAT) has been highlighted as a new possible therapeutic target for obesity, diabetes and lipid metabolic disorders, because WAT browning could increase energy expenditure and reduce adiposity. The new clusters of adipocytes that emerge with WAT browning have been named 'beige' or 'brite' adipocytes. Recent reports have indicated that the renin-angiotensin system (RAS) plays a role in various aspects of adipose tissue physiology and dysfunction. The biological effects of angiotensin II, a major component of RAS, are mediated by two receptor subtypes, angiotensin II type 1 receptor (AT1R) and type 2 receptor (AT2R)...
2016: PloS One
https://www.readbyqxmd.com/read/27987402/efficacy-of-human-umbilical-cord-derived-mesenchymal-stem-cells-in-treatment-of-rat-bone-marrow-exposed-to-gamma-irradiation
#13
Hanaa S E Mousa, Sally M Shalaby, Zienab A Gouda, Fayza E Ahmed, Aisha A El-Khodary
To assess the therapeutic effects of the human umbilical cord blood (hUCB) derived mesenchymal stem cells (MSCs) on rat bone marrow (BM) exposed to gamma rays, 3 groups (n=15 each) of adult male Wistar albino rats were utilized as follows: the 1st group received PBS (control group), the 2nd group was exposed to gamma rays 1.04Gy/min (R group) and the 3rd group exposed to same dose as RG and injected hUCB-MSCs. The BM of femurs was processed for histological and immunohistochemical staining with proliferating cell nuclear antigen antibody (PCNA), anti human CD105 and anti human CD34...
December 14, 2016: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/27956229/milk-fat-globule-egf-factor-8-secreted-by-mesenchymal-stem-cells-protects-against-liver-fibrosis-in-mice
#14
Su Yeon An, Yu Jin Jang, Hee-Joung Lim, Jiyou Han, Jaehun Lee, Gyunggyu Lee, Ji Young Park, Seo-Young Park, Ji Hyang Kim, Byung-Rok Do, Chugseong Han, Hee-Kyung Park, Ok-Hee Kim, Myeong Jun Song, Say-June Kim, Jong-Hoon Kim
BACKGROUND & AIMS: Mesenchymal stem cells (MSCs) mediate tissue repair and might be used to prevent or reduce liver fibrosis. However, little is known about the anti-fibrotic factors secreted from MSCs or their mechanisms. METHODS: Umbilical cord-derived MSCs (UCMSCs) were differentiated into hepatocyte-like cells (hpUCMSCs), medium was collected, and secretome proteins were identified and quantified using nanochip-liquid chromatography/quadruple time-of-flight mass spectrometry...
December 9, 2016: Gastroenterology
https://www.readbyqxmd.com/read/27942383/autologous-fat-transplantation-for-breast-reconstruction-a-literature-review
#15
REVIEW
Francesco Simonacci, Nicolò Bertozzi, Michele Pio Grieco, Eugenio Grignaffini, Edoardo Raposio
INTRODUCTION: The use of autologous fat transplantation to correct volume and contour defects, scars, and asymmetry after breast cancer surgery has increased over the past 20 years. Many developments and refinements in this technique have taken place in recent years, and several studies of the safety of lipofilling in the breast have been published. PRESENTATION OF CASE: We performed a literature review of this technique, highlighting the crucial role of lipofilling in breast cancer reconstruction...
December 2016: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/27937753/isolation-and-characterization-of-multipotent-mesenchymal-stem-cells-adhering-to-adipocytes-in-canine-bone-marrow
#16
Hsing-Yi Lin, Naoki Fujita, Kentaro Endo, Maresuke Morita, Tae Takeda, Takayuki Nakagawa, Ryohei Nishimura
The ceiling culture method has been used to isolate mature adipocytes from adipose tissue that can be dedifferentiated into fibroblastic cells, also known as dedifferentiated fat (DFAT) cells that self-renew and are multipotent, with much higher homogeneity and colony-forming efficiency than those of adipose tissue-derived mesenchymal stem cells. We cultured adipocytes from canine bone marrow using this technique, with the expectation of obtaining DFAT cells. However, contrary to our expectations, continuous monitoring of ceiling cultures by time-lapse microscopy revealed many small cells adhering to adipocytes that proliferated rapidly into cells with a fibroblastic morphology and without any dedifferentiation from adipocytes...
December 3, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27921032/the-collection-of-adipose-derived-stem-cells-using-water-jet-assisted-lipoplasty-for-their-use-in-plastic-and-reconstructive-surgery-a-preliminary-study
#17
Valeria Purpura, Elena Bondioli, Davide Melandri, Pier C Parodi, Luca Valenti, Michele Riccio
The graft of autologous fat for the augmentation of soft tissue is a common practice frequently used in the field of plastic and reconstructive surgery. In addition, the presence of adipose derived stem cells (ASCs) in adipose tissue stimulates the regeneration of tissue in which it is applied after the autologous fat grafting improving the final clinical results. Due to these characteristics, there is an increasing interest in the use of ASCs for the treatment of several clinical conditions. As a consequence, the use of clean room environment is required for the production of cell-based therapies...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27920882/paroxetine-can-enhance-neurogenesis-during-neurogenic-differentiation-of-human-adipose-derived-stem-cells
#18
Maliheh Jahromi, Shahnaz Razavi, Nushin Amirpour, Zahra Khosravizadeh
BACKGROUND: Some antidepressant drugs can promote neuronal cell proliferation in vitro as well as hippocampal neurogenesis in human and animal models. Furthermore, adipose tissue is an available source of adult stem cells with the ability to differentiate in to multiple lineages. Therefore, human Adipose-Derived Stem Cells (hAD-SCs) may be a suitable source for regenerative medical applications. Since there is no evidence for the effect of Paroxetine as the most commonly prescribed antidepressant drug for neurogenic potential of hADSCs, an attempt was made to determine the effect of Paroxetine on proliferation and neural differentiation of hADSCs...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27906881/stem-cells-and-clinical-practice-new-advances-and-challenges-at-the-time-of-emerging-problems-with-induced-pluripotent-stem-cell-therapies
#19
Mariusz Z Ratajczak, Kamila Bujko, Wojciech Wojakowski
Humans, like other species that reproduce sexually, originate from a fertilized oocyte (zygote), which is a totipotent stem cell giving rise to an adult organism. During the process of embryogenesis, stem cells at different levels of the developmental hierarchy establish all 3 germ layers and give rise to tissue‑committed stem cells, which are responsible for rejuvenation of a given tissue or organ. The robustness of the stem cell compartment is one of the major factors that directly impact life quality as well as lifespan...
November 10, 2016: Polskie Archiwum Medycyny Wewnętrznej
https://www.readbyqxmd.com/read/27906056/umbilical-cord-blood-derived-non-hematopoietic-stem-cells-retrieved-and-expanded-on-bone-marrow-derived-extracellular-matrix-display-pluripotent-characteristics
#20
Junjie Wu, Yun Sun, Travis J Block, Milos Marinkovic, Zhi-Liang Zhang, Richard Chen, Yixia Yin, Juquan Song, David D Dean, Zhongding Lu, Xiao-Dong Chen
BACKGROUND: Umbilical cord blood (UCB) not only contains hematopoietic stem cells (HSCs), but also non-hematopoietic stem cells (NHSCs) that are able to differentiate into a number of distinct cell types. Based on studies published to date, the frequency of NHSCs in UCB is believed to be very low. However, the isolation of these cells is primarily based on their adhesion to tissue culture plastic surfaces. METHODS AND RESULTS: In the current study, we demonstrate that this approach overlooks some of the extremely immature NHSCs because they lack the ability to adhere to plastic...
December 1, 2016: Stem Cell Research & Therapy
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