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https://www.readbyqxmd.com/read/28818899/draft-genome-sequence-of-sphingobacterium-sp-cz-uam-isolated-from-a-methanotrophic-consortium
#1
José Luis Steffani-Vallejo, Cristal Zuñiga, Pablo Cruz-Morales, Luis Lozano, Marcia Morales, Cuauhtemoc Licona-Cassani, Sergio Revah, José Utrilla
Sphingobacterium sp. CZ-UAM was isolated from a methanotrophic consortium in mineral medium using methane as the only carbon source. A draft genome of 5.84 Mb with a 40.77% G+C content is reported here. This genome sequence will allow the investigation of potential methanotrophy in this isolated strain.
August 17, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28789800/rapid-ultrasonic-and-microwave-assisted-micellar-extraction-of-zingiberone-shogaol-and-gingerols-from-gingers-using-biosurfactants
#2
Li-Qing Peng, Jun Cao, Li-Jing Du, Qi-Dong Zhang, Jing-Jing Xu, Yu-Bo Chen, Yu-Ting Shi, Rong-Rong Li
Two kinds of extraction methods ultrasonic-assisted micellar extraction (UAME) and microwave-assisted micellar extraction (MAME) coupled with ultra-high performance liquid chromatography with ultraviolet detector (UHPLC-UV) were developed and evaluated for extraction and determination of zingerone, 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol in Rhizoma Zingiberis and Rhizoma Zingiberis Preparata. A biosurfactant, hyodeoxycholic acid sodium salt, was used in micellar extraction. Several experimental parameters were studied separately by a univariate method...
August 1, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28655399/sonochemical-synthesis-of-highly-crystalline-photocatalyst-for-industrial-applications
#3
Muhammad Tayyab Noman, Jiri Militky, Jakub Wiener, Jana Saskova, Muhammad Azeem Ashraf, Hafsa Jamshaid, Musaddaq Azeem
Highly photo active pure anatase form of TiO2 nanoparticles with average particle size 4nm have been successfully synthesized by ultrasonic acoustic method (UAM). The effects of process variables i.e. precursors concentration and sonication time were investigated based on central composite design and response surface methodology. The characteristics of the resulting nanoparticles (RNP) were analyzed by scanning electron microscopy, dynamic light scattering, transmission electron microscopy, X-ray diffractometry and Raman spectroscopy...
June 20, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28448019/a-tandem-liquid-chromatography-mass-spectrometry-based-approach-for-metabolite-analysis-of-staphylococcus-aureus
#4
David J Samuels, Zhe Wang, Kyu Y Rhee, Shaun R Brinsmade
In an effort to thwart bacterial pathogens, hosts often limit the availability of nutrients at the site of infection. This limitation can alter the abundances of key metabolites to which regulatory factors respond, adjusting cellular metabolism. In recent years, a number of proteins and RNA have emerged as important regulators of virulence gene expression. For example, the CodY protein responds to levels of branched-chain amino acids and GTP and is widely conserved in low G+C Gram-positive bacteria. As a global regulator in Staphylococcus aureus, CodY controls the expression of dozens of virulence and metabolic genes...
March 28, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28368284/x-ray-diffraction-analysis-and-in-vitro-characterization-of-the-uam2-protein-from-oryza-sativa
#5
Ditte Hededam Welner, Alex Yi Lin Tsai, Andy M DeGiovanni, Henrik Vibe Scheller, Paul D Adams
The role of seemingly non-enzymatic proteins in complexes interconverting UDP-arabinopyranose and UDP-arabinofuranose (UDP-arabinosemutases; UAMs) in the plant cytosol remains unknown. To shed light on their function, crystallographic and functional studies of the seemingly non-enzymatic UAM2 protein from Oryza sativa (OsUAM2) were undertaken. Here, X-ray diffraction data are reported, as well as analysis of the oligomeric state in the crystal and in solution. OsUAM2 crystallizes readily but forms highly radiation-sensitive crystals with limited diffraction power, requiring careful low-dose vector data acquisition...
April 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28269237/defining-new-aims-for-bme-programs-in-latin-america-the-case-of-uam-iztapalapa
#6
J Azpiroz-Leehan, L F Martinez, M E G Urbina, M M Cadena, E Sacristan
The need for upkeep and management of medical technology has fostered the creation of a large number of under graduate programs in the field of biomedical Engineering. In Latin America alone, there are over 85 programs dedicated to this. This contrasts with programs in other regions where most of the undergraduates continue on to pursue graduate degrees or work as research and development engineers in the biomedical industry. In this work we analyze the situation regarding curricular design in the 48 BME programs in Mexico and compare this to suggestions and classifications of programs according to needs and possibilities...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227471/defining-new-aims-for-bme-programs-in-latin-america-the-case-of-uam-iztapalapa
#7
J Azpiroz-Leehan, L F Martinez, M E G Urbina, M M Cadena, E Sacristan, J Azpiroz-Leehan, L F Martinez, M E G Urbina, M M Cadena, E Sacristan, M M Cadena, M E G Urbina, E Sacristan, J Azpiroz-Leehan, L F Martinez
The need for upkeep and management of medical technology has fostered the creation of a large number of under graduate programs in the field of biomedical Engineering. In Latin America alone, there are over 85 programs dedicated to this. This contrasts with programs in other regions where most of the undergraduates continue on to pursue graduate degrees or work as research and development engineers in the biomedical industry. In this work we analyze the situation regarding curricular design in the 48 BME programs in Mexico and compare this to suggestions and classifications of programs according to needs and possibilities...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28192204/analysis-of-plant-udp-arabinopyranose-mutase-uam-role-of-divalent-metals-and-structure-prediction
#8
Jijin R A Kuttiyatveetil, David A R Sanders
UDP-arabinopyranose mutase (UAM) is a plant enzyme which interconverts UDP-arabinopyranose (UDP-Arap; a six-membered sugar) to UDP-arabinofuranose (UDP-Araf; a five-membered sugar). Plant mutases belong to a small gene family called Reversibly Glycosylated Proteins (RGPs). So far, UAM has been identified in Oryza sativa (Rice), Arabidopsis thaliana and Hordeum vulgare (Barley). The enzyme requires divalent metal ions for catalytic activity. Here, the divalent metal ion dependency of UAMs from O. sativa (rice) and A...
May 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28106469/building-a-dna-barcode-library-of-alaska-s-non-marine-arthropods
#9
Derek S Sikes, Matthew Bowser, John M Morton, Casey Bickford, Sarah Meierotto, Kyndall Hildebrandt
Climate change may result in ecological futures with novel species assemblages, trophic mismatch, and mass extinction. Alaska has a limited taxonomic workforce to address these changes. We are building a DNA barcode library to facilitate a metabarcoding approach to monitoring non-marine arthropods. Working with the Canadian Centre for DNA Barcoding, we obtained DNA barcodes from recently collected and authoritatively identified specimens in the University of Alaska Museum (UAM) Insect Collection and the Kenai National Wildlife Refuge collection...
March 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27957429/relationship-between-level-of-hba1c-and-breast-cancer
#10
Fariba Jousheghany, Joshua Phelps, Tina Crook, Reza Hakkak
BACKGROUND: Diabetes and cancer are public health issues worldwide; studies have shown that diabetes is related to increased breast cancer mortality. The purpose of this study was to examine associations between HbA1C and obesity with tumor stage and mortality among breast cancer patients. METHODS: Data for 82 patients with breast cancer (36-89 years of age, diagnosed /treated 1999-2009) were provided by the University of Arkansas for Medical Sciences (UAMS) Data Trust Warehouse...
December 2016: BBA Clinical
https://www.readbyqxmd.com/read/27928821/the-universal-anaesthesia-machine-uam-assessment-of-a-new-anaesthesia-workstation-built-with-global-health-in-mind
#11
D A H de Beer, F D Nesbitt, G T Bell, A Rapuleng
The Universal Anaesthesia Machine has been developed as a complete anaesthesia workstation for use in low- and middle-income countries, where the provision of safe general anaesthesia is often compromised by unreliable supply of electricity and anaesthetic gases. We performed a functional and clinical assessment of this anaesthetic machine, with particular reference to novel features and functioning in the intended environment. The Universal Anaesthesia Machine was found to be reliable, safe and consistent across a range of tests during targeted functional testing...
April 2017: Anaesthesia
https://www.readbyqxmd.com/read/27907126/variability-in-the-sxt-gene-clusters-of-psp-toxin-producing-aphanizomenon-gracile-strains-from-norway-spain-germany-and-north-america
#12
Andreas Ballot, Leonardo Cerasino, Vladyslava Hostyeva, Samuel Cirés
Paralytic shellfish poisoning (PSP) toxin production has been detected worldwide in the cyanobacterial genera Anabaena, Lyngbya, Scytonema, Cuspidothrix and Aphanizomenon. In Europe Aphanizomenon gracile and Cuspidothrix issatschenkoi are the only known producers of PSP toxins and are found in Southwest and Central European freshwater bodies. In this study the PSP toxin producing Aphanizomenon sp. strain NIVA-CYA 851 was isolated from the Norwegian Lake Hillestadvannet. In a polyphasic approach NIVA-CYA 851 was morphologically and phylogenetically classified, and investigated for toxin production...
2016: PloS One
https://www.readbyqxmd.com/read/27833622/downregulation-of-a-udp-arabinomutase-gene-in-switchgrass-panicum-virgatum-l-results-in-increased-cell-wall-lignin-while-reducing-arabinose-glycans
#13
Jonathan D Willis, James A Smith, Mitra Mazarei, Ji-Yi Zhang, Geoffrey B Turner, Stephen R Decker, Robert W Sykes, Charleson R Poovaiah, Holly L Baxter, David G J Mann, Mark F Davis, Michael K Udvardi, Maria J Peña, Jason Backe, Maor Bar-Peled, C N Stewart
Background: Switchgrass (Panicum virgatum L.) is a C4 perennial prairie grass and a dedicated feedstock for lignocellulosic biofuels. Saccharification and biofuel yields are inhibited by the plant cell wall's natural recalcitrance against enzymatic degradation. Plant hemicellulose polysaccharides such as arabinoxylans structurally support and cross-link other cell wall polymers. Grasses predominately have Type II cell walls that are abundant in arabinoxylan, which comprise nearly 25% of aboveground biomass...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27758049/quantitative-identification-of-compound-dependent-on-modules-and-differential-allosteric-modules-from-homologous-ischemic-networks
#14
B Li, J Liu, Y Y Zhang, P Q Wang, Y N Yu, R X Kang, H L Wu, X X Zhang, Z Wang, Y Y Wang
Module-based methods have made much progress in deconstructing biological networks. However, it is a great challenge to quantitatively compare the topological structural variations of modules (allosteric modules [AMs]) under different situations. A total of 23, 42, and 15 coexpression modules were identified in baicalin (BA), jasminoidin (JA), and ursodeoxycholic acid (UA) in a global anti-ischemic mice network, respectively. Then, we integrated the methods of module-based consensus ratio (MCR) and modified Zsummary module statistic to validate 12 BA, 22 JA, and 8 UA on-modules based on comparing with vehicle...
October 2016: CPT: Pharmacometrics & Systems Pharmacology
https://www.readbyqxmd.com/read/27622146/antibiotic-loaded-phosphatidylcholine-inhibits-staphylococcal-bone-infection
#15
Jessica Amber Jennings, Karen E Beenken, Robert A Skinner, Daniel G Meeker, Mark S Smeltzer, Warren O Haggard, Karen S Troxel
AIM: To test antibiotic-loaded coating for efficacy in reducing bacterial biofilm and development of osteomyelitis in an orthopaedic model of implant infection. METHODS: Phosphatidylcholine coatings loaded with 25% vancomycin were applied to washed and sterilized titanium wires 20 mm in length. A 10 mm segment was removed from rabbit radius (total = 9; 5 coated, 4 uncoated), and the segment was injected with 1 × 10(6) colony forming units (CFUs) of Staphylococcus aureus (UAMS-1 strain)...
August 18, 2016: World Journal of Orthopedics
https://www.readbyqxmd.com/read/27611492/dynamics-of-ultrasonic-additive-manufacturing
#16
Adam Hehr, Marcelo J Dapino
Ultrasonic additive manufacturing (UAM) is a solid-state technology for joining similar and dissimilar metal foils near room temperature by scrubbing them together with ultrasonic vibrations under pressure. Structural dynamics of the welding assembly and work piece influence how energy is transferred during the process and ultimately, part quality. To understand the effect of structural dynamics during UAM, a linear time-invariant model is proposed to relate the inputs of shear force and electric current to resultant welder velocity and voltage...
August 17, 2016: Ultrasonics
https://www.readbyqxmd.com/read/27503543/development-and-application-of-ultrasound-assisted-microextraction-to-analysis-of-fenitrothion-in-environmental-samples
#17
Fumiki Takahashi, Kanya Kobayashi, Jiye Jin
A microextraction technique based on ultrasonic emulsification and demulsification was developed for detecting pesticides at trace levels in environmental water samples. In this ultrasound-assisted microextraction (UAME), chloroform was emulsified with an aqueous sample solution containing trace fenitrothion (MEP) by ultrasonic irradiation (48 kHz) for 5 min. The emulsion was then demulsified by ultrasonic irradiation (2.4 MHz) for 10 min. This resulted in phase separation of the water and chloroform without centrifugation...
October 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27401574/evaluation-of-antibiotics-active-against-methicillin-resistant-staphylococcus-aureus-based-on-activity-in-an-established-biofilm
#18
Daniel G Meeker, Karen E Beenken, Weston B Mills, Allister J Loughran, Horace J Spencer, William B Lynn, Mark S Smeltzer
We used in vitro and in vivo models of catheter-associated biofilm formation to compare the relative activity of antibiotics effective against methicillin-resistant Staphylococcus aureus (MRSA) in the specific context of an established biofilm. The results demonstrated that, under in vitro conditions, daptomycin and ceftaroline exhibited comparable activity relative to each other and greater activity than vancomycin, telavancin, oritavancin, dalbavancin, or tigecycline. This was true when assessed using established biofilms formed by the USA300 methicillin-resistant strain LAC and the USA200 methicillin-sensitive strain UAMS-1...
October 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27375189/an-apolar-pistacia-lentiscus-l-leaf-extract-gc-ms-metabolic-profiling-and-evaluation-of-cytotoxicity-and-apoptosis-inducing-effects-on-sh-sy5y-and-sk-n-be-2-c-cell-lines
#19
Simona Piccolella, Paola Nocera, Petronia Carillo, Pasqualina Woodrow, Vincenza Greco, Lorenzo Manti, Antonio Fiorentino, Severina Pacifico
In the course of a cytotoxicity screening of Mediterranean plants vs. neuroblastoma cells, Pistacia lentiscus was of interest. Pl-C extract, prepared from dried leaves by ultrasound assisted maceration (UAM) in chloroform, was profiled through using GC-MS techniques. To evaluate Pl-C cytotoxicity towards SH-SY5Y and SK-N-BE(2)-C cell lines, MTT, SRB and LDH assays were performed. The caspase-3 activation, DNA fragmentation, as well as micronucleation, were also evaluated. The Pl-C oxidant/antioxidant ability was estimated using different methods...
September 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27354444/impact-of-sara-and-phenol-soluble-modulins-on-the-pathogenesis-of-osteomyelitis-in-diverse-clinical-isolates-of-staphylococcus-aureus
#20
Allister J Loughran, Dana Gaddy, Karen E Beenken, Daniel G Meeker, Roy Morello, Haibo Zhao, Stephanie D Byrum, Alan J Tackett, James E Cassat, Mark S Smeltzer
We used a murine model of acute, posttraumatic osteomyelitis to evaluate the virulence of two divergent Staphylococcus aureus clinical isolates (the USA300 strain LAC and the USA200 strain UAMS-1) and their isogenic sarA mutants. The results confirmed that both strains caused comparable degrees of osteolysis and reactive new bone formation in the acute phase of osteomyelitis. Conditioned medium (CM) from stationary-phase cultures of both strains was cytotoxic to cells of established cell lines (MC3TC-E1 and RAW 264...
September 2016: Infection and Immunity
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