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https://www.readbyqxmd.com/read/27909235/a-simple-and-sensitive-high-content-assay-for-the-characterization-of-antiproliferative-therapeutic-antibodies
#1
Andreas Stengl, David Hörl, Heinrich Leonhardt, Jonas Helma
Monoclonal antibodies (mAbs) have become a central class of therapeutic agents in particular as antiproliferative compounds. Their often complex modes of action require sensitive assays during early, functional characterization. Current cell-based proliferation assays often detect metabolites that are indicative of metabolic activity but do not directly account for cell proliferation. Measuring DNA replication by incorporation of base analogues such as 5-bromo-2'-deoxyuridine (BrdU) fills this analytical gap but was previously restricted to bulk effect characterization in enzyme-linked immunosorbent assay formats...
December 1, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27901205/six-month-color-change-and-water-sorption-of-9-new-generation-flowable-composites-in-6-staining-solutions
#2
Maria Arregui, Luis Giner, Marco Ferrari, Marta Vallés, Montserrat Mercadé
Color match and water sorption are two factors that affect restorative materials. Discoloration is essential in the lifespan of restorations. The aim of this study was to evaluate color change and water sorption of nine flowable composites at multiple time points over 6 months. 60 samples of each composite were divided into two groups (Color Change and Water Sorption/Solubility). Each Color Change group was divided into six subgroups, which were immersed in distilled water (DW), coffee (CF), Coca-Cola (CC), red wine (RW), tea (TE) and orange juice (OJ)...
November 28, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27892839/influence-of-adhesive-type-and-placement-technique-on-postoperative-sensitivity-in-posterior-composite-restorations
#3
Trf Costa, M Rezende, A Sakamoto, B Bittencourt, P Dalzochio, A D Loguercio, A Reis
PURPOSE: This double blind, randomized clinical trial compared the postoperative sensitivity of the placement technique (incremental and bulk fill) in posterior composite resin restorations bonded with two different adhesive strategies (self-etch and etch-and-rinse). METHODS: Posterior dental cavities of 72 participants (n=236), with a cavity depth of at least 3 mm, were randomly divided into four groups. The restorations were bonded using either the etch-and-rinse Tetric N-Bond (Ivoclar Vivadent) or the self-etch Tetric N-Bond SE (Ivoclar Vivadent)...
November 28, 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27892836/comparison-of-internal-adaptation-in-class-ii-bulk-fill-composite-restorations-using-micro-ct
#4
S H Han, S H Park
PURPOSE: This study compared the internal adaptation of bulk-fill composite restorations in class II cavities and explored the relationship between internal adaptation and polymerization shrinkage or stress. METHODS AND MATERIALS: Standardized mesio-occluso-distal cavities were prepared in 40 extracted human third molars and randomly divided into five groups (n=8). After having been applied by total-etch XP bond (Dentsply Caulk, Milford, DE, USA) and light curing, the teeth were restored with the following resin composites: group 1, Filtek Z350 (3M ESPE, St...
November 28, 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27886774/analysis-of-tricyclic-antidepressants-in-human-plasma-using-online-restricted-access-molecularly-imprinted-solid-phase-extraction-followed-by-direct-mass-spectrometry-identification-quantification
#5
Mariane Gonçalves Santos, Isabela Maria Campos Tavares, Adriano Francisco Barbosa, Jefferson Bettini, Eduardo Costa Figueiredo
The use of a new class of hybrid materials, called restricted access molecularly imprinted polymers (RAMIPs) seems to present a good strategy for the sample preparation of complex matrices, since these materials combine good protein elimination capacity with high degree selectivity. Mass spectrometers (MS) have been successfully used for polar drug identification and quantification. In order to combine the advantages of both RAMIPs and mass spectrometry, we proposed a study that joins these properties in a single system, where we could analyse tricyclic antidepressants from human plasma, without offline extraction or chromatographic separation...
January 15, 2017: Talanta
https://www.readbyqxmd.com/read/27885743/influence-of-polymerisation-method-and-type-of-fibre-on-fracture-strength-of-endodontically-treated-teeth
#6
Neslihan Tekçe, Kansad Pala, Safa Tuncer, Mustafa Demirci, Merve Efe Serim
The aim of this study was to investigate (i) the effect of direct or indirect polymerisation of adhesive-impregnated ribbon fibre under 4-mm bulk-filled composites on fracture strength; (ii) to compare polyethylene ribbon fibre-reinforcement composites with short fibre-reinforced composite; and (iii) the effectiveness of polyethylene ribbon fibre according to the restorative materials used (low-viscous bulk-fill composite, high-viscous flowable composite or conventional paste composite). Seventy molars were divided into seven groups; (groups 1-2) Ribbond-reinforced Surefil-SDR; (group 3) Ribbond-reinforced G Aenial Flo; (group 4) Ribbond-reinforced G Aenial Posterior; (group 5) short fibre-reinforced composite everX Posterior; (group 6) unfilled cavity; (group 7) intact teeth...
November 25, 2016: Australian Endodontic Journal: the Journal of the Australian Society of Endodontology Inc
https://www.readbyqxmd.com/read/27882021/compressive-strength-and-the-effect-of-duration-after-photo-activation-among-dual-cure-bulk-fill-composite-core-materials
#7
Fahad Alkhudhairy, Fahim Vohra
OBJECTIVES: To assess compressive strength and effect of duration after photoactivation on the compressive strength of different dual cure bulk fill composites. METHODS: Seventy-two disc shaped (4x10mm) specimens were prepared from three dual cure bulk fill materials, ZirconCore (ZC) (n=24), MulticCore Flow (MC) (n=24) and Luxacore Dual (LC) (n=24). Half of the specimens in each material were tested for failure loads after one hour [MC1 (n=12), LC1 (n=12) & ZC1 (n=12)] and the other half in 7 days [MC7 (n=12), LC7 (n=12), ZC7 (n=12)] from photo-polymerization using the universal testing machine at a cross-head speed of 0...
September 2016: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/27878177/gate-controlled-conductance-enhancement-from-quantum-hall-channels-along-graphene-p-n-junctions
#8
Endre Tóvári, Péter Makk, Ming-Hao Liu, Peter Rickhaus, Zoltán Kovács-Krausz, Klaus Richter, Christian Schönenberger, Szabolcs Csonka
The formation of quantum Hall channels inside the bulk of graphene is studied using various contact and gate geometries. p-n junctions are created along the longitudinal direction of samples, and enhanced conductance is observed in the case of bipolar doping due to the new conducting channels formed in the bulk, whose position, propagating direction and, in one geometry, coupling to electrodes are determined by the gate-controlled filling factor across the device. This effect could be exploited to probe the behavior and interaction of quantum Hall channels protected against uncontrolled scattering at the edges...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27843942/color-matching-and-blending-effect-of-universal-shade-bulk-fill-resin-composite-in-resin-composite-models-and-natural-teeth
#9
Rasha M Abdelraouf, Nour A Habib
Objectives. To assess visually color-matching and blending-effect (BE) of a universal shade bulk-fill-resin-composite placed in resin-composite-models with different shades and cavity sizes and in natural teeth (extracted and patients' teeth). Materials and Methods. Resin-composite-discs (10 mm × 1 mm) were prepared of universal shade composite and resin-composite of shades: A1, A2, A3, A3.5, and A4. Spectrophotometric-color-measurement was performed to calculate color-difference (ΔE) between the universal shade and shaded-resin-composites discs and determine their translucency-parameter (TP)...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27836116/effect-of-layer-thickness-on-the-elution-of-bulk-fill-composite-components
#10
Lena Rothmund, Franz-Xaver Reichl, Reinhard Hickel, Panorea Styllou, Marianthi Styllou, Kai Kehe, Yang Yang, Christof Högg
OBJECTIVE: An increment layering technique in a thickness of 2mm or less has been the standard to sufficiently convert (co)monomers. Bulk fill resin composites were developed to accelerate the restoration process by enabling up to 4mm thick increments to be cured in a single step. The aim of the present study is to investigate the effect of layer thickness on the elution of components from bulk fill composites. METHODS: The composites ELS Bulk fill, SDR Bulk fill and Venus Bulkfill were polymerized according to the instruction of the manufacturers...
November 8, 2016: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/27836035/detection-and-risk-factors-of-coxiella-burnetii-infection-in-dairy-cattle-based-on-bulk-tank-milk-samples-in-center-of-iran
#11
Zary Nokhodian, Awat Feizi, Abdolreza Moradi, Majid Yaran, Shervin Ghaffari Hoseini, Behrooz Ataei, Mohammad Hosseini
Infection with Coxiella burnetii has a worldwide distribution in ruminants and is associated with abortions and reproductive problems in this group of animals. This study aimed to determine the prevalence of C. burnetii DNA in bulk tank milk (BTM) of dairy cattle herds and to identify the risk factors of infection. This cross-sectional study was conducted in spring 2015. A total of 163 BTM samples from 74 commercial and 89 traditional dairy cattle herds in Isfahan, Iran was tested for the IS1111 transposon of C...
November 1, 2016: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/27835026/unconventional-correlation-between-quantum-hall-transport-quantization-and-bulk-state-filling-in-gated-graphene-devices
#12
Yong-Tao Cui, Bo Wen, Eric Y Ma, Georgi Diankov, Zheng Han, Francois Amet, Takashi Taniguchi, Kenji Watanabe, David Goldhaber-Gordon, Cory R Dean, Zhi-Xun Shen
We report simultaneous transport and scanning microwave impedance microscopy to examine the correlation between transport quantization and filling of the bulk Landau levels in the quantum Hall regime in gated graphene devices. Surprisingly, a comparison of these measurements reveals that quantized transport typically occurs below the complete filling of bulk Landau levels, when the bulk is still conductive. This result points to a revised understanding of transport quantization when carriers are accumulated by gating...
October 28, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27822296/risk-assessment-for-job-burnout-with-a-mobile-health-web-application-using-questionnaire-data-a-proof-of-concept-study
#13
Roland von Känel, Marc van Nuffel, Walther J Fuchs
BACKGROUND: Job burnout has become a rampant epidemic in working societies, causing high productivity loss and healthcare costs. An easy accessible tool to detect clinically relevant risk may bear the potential to timely avert the dire sequelae of burnout. As a start, we performed a proof of concept study to test the utilization of a mobile health web application for a free and anonymous burnout risk assessment with established questionnaires. METHODS: We designed a client-side javascript web application for users who filled out demographic and psychometric data forms over the internet...
2016: BioPsychoSocial Medicine
https://www.readbyqxmd.com/read/27820696/influence-of-the-compliance-and-layering-method-on-the-wall-deflection-of-simulated-cavities-in-bulk-fill-composite-restoration
#14
Y-J Kim, R Kim, J L Ferracane, I-B Lee
The aim of this study was to investigate the effects of the layering method and compliance on the wall deflection of simulated cavities in bulk-fill and conventional composite restorations and to examine the relationships between the wall deflection and the polymerization shrinkage, flexural modulus, and polymerization shrinkage stress of composites. Six light-cured composites were used in this study. Two of these were conventional methacrylate-based composites (Filtek Z250 and Filtek Z350 XT Flowable [Z350F]), whereas four were bulk-fill composites (SonicFill, Tetric N-Ceram Bulk-Fill, SureFil SDR Flow [SDR], and Filtek Bulk-Fill)...
November 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27820694/degradation-potential-of-bulk-versus-incrementally-applied-and-indirect-composites-color-microhardness-and-surface-deterioration
#15
M El Gezawi, D Kaisarly, H Al-Saleh, A ArRejaie, F Al-Harbi, K H Kunzelmann
This study investigated the color stability and microhardness of five composites exposed to four beverages with different pH values. Composite discs were produced (n=10); Filtek Z250 (3M ESPE) and Filtek P90 (3M ESPE) were applied in two layers (2 mm, 20 seconds), and Tetric N-Ceram Bulk Fill (TetricBF, Ivoclar Vivadent) and SonicFill (Kerr) were applied in bulk (4 mm) and then light cured (40 seconds, Ortholux-LED, 1600 mW/cm(2)). Indirect composite Sinfony (3M ESPE) was applied in two layers (2 mm) and cured (Visio system, 3M ESPE)...
November 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27798031/the-environmental-footprint-of-morphine-a-life-cycle-assessment-from-opium-poppy-farming-to-the-packaged-drug
#16
Scott McAlister, Yanjun Ou, Elise Neff, Karen Hapgood, David Story, Philip Mealey, Forbes McGain
OBJECTIVE: To examine the environmental life cycle from poppy farming through to production of 100 mg in 100 mL of intravenous morphine (standard infusion bag). DESIGN: 'Cradle-to-grave' process-based life cycle assessment (observational). SETTINGS: Australian opium poppy farms, and facilities for pelletising, manufacturing morphine, and sterilising and packaging bags of morphine. MAIN OUTCOME MEASURES: The environmental effects (eg, CO2 equivalent ('CO2 e') emissions and water use) of producing 100 mg of morphine...
October 21, 2016: BMJ Open
https://www.readbyqxmd.com/read/27792380/topological-varma-superfluid-in-optical-lattices
#17
M Di Liberto, A Hemmerich, C Morais Smith
Topological states of matter are peculiar quantum phases showing different edge and bulk transport properties connected by the bulk-boundary correspondence. While noninteracting fermionic topological insulators are well established by now and have been classified according to a tenfold scheme, the possible realization of topological states for bosons has not been explored much yet. Furthermore, the role of interactions is far from being understood. Here, we show that a topological state of matter exclusively driven by interactions may occur in the p band of a Lieb optical lattice filled with ultracold bosons...
October 14, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27792364/fermi-surface-of-metallic-v_-2-o_-3-from-angle-resolved-photoemission-mid-level-filling-of-e_-g-%C3%AF-bands
#18
I Lo Vecchio, J D Denlinger, O Krupin, B J Kim, P A Metcalf, S Lupi, J W Allen, A Lanzara
Using angle resolved photoemission spectroscopy, we report the first band dispersions and distinct features of the bulk Fermi surface (FS) in the paramagnetic metallic phase of the prototypical metal-insulator transition material V_{2}O_{3}. Along the c axis we observe both an electron pocket and a triangular holelike FS topology, showing that both V 3d a_{1g} and e_{g}^{π} states contribute to the FS. These results challenge the existing correlation-enhanced crystal field splitting theoretical explanation for the transition mechanism and pave the way for the solution of this mystery...
October 14, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27783075/counterbalancing-of-morphology-and-conductivity-of-poly-3-4-ethylenedioxythiophene-polystyrene-sulfonate-based-flexible-devices
#19
Woongsik Jang, Sunyong Ahn, Soyun Park, Jong Hyeok Park, Dong Hwan Wang
The importance of conductive polymer electrodes with a balance between the morphology and electrical conductivity for flexible organic photovoltaic properties has been demonstrated. Highly transparent PEDOT:PSS anodes with controlled conductivity and surface properties were realized by insertion of dimethyl sulfoxide (DMSO) and a fluorosurfactant (Zonyl) as efficient additives and used for flexible organic photovoltaic cells (OPVs) which are based on a bulk-heterojunction of polythieno[3,4-b]-thiophene-co-benzodithiophene (PTB7):[6,6]phenyl-C71-butyric acid methyl ester (PC71BM)...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27775127/composite-foods-from-structure-to-sensory-perception
#20
Elke Scholten
An understanding of the effect of structural features of foods in terms of specific sensory attributes is necessary to design foods with specific functionalities, such as reduced fat or increased protein content, and increased feeling of satiety or liking. Although the bulk rheological properties of both liquid and solid foods can be related to textural attributes such as thickness and firmness, they do not always correlate to more complex sensory attributes, such as creamy and smooth. These attributes are often a result of different contributions, including lubrication aspects and interactions between food and components present in the oral cavity...
October 24, 2016: Food & Function
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