keyword
https://read.qxmd.com/read/37884489/small-leucine-rich-proteoglycans-inhibit-cns-regeneration-by-modifying-the-structural-and-mechanical-properties-of-the-lesion-environment
#21
JOURNAL ARTICLE
Julia Kolb, Vasiliki Tsata, Nora John, Kyoohyun Kim, Conrad Möckel, Gonzalo Rosso, Veronika Kurbel, Asha Parmar, Gargi Sharma, Kristina Karandasheva, Shada Abuhattum, Olga Lyraki, Timon Beck, Paul Müller, Raimund Schlüßler, Renato Frischknecht, Anja Wehner, Nicole Krombholz, Barbara Steigenberger, Dimitris Beis, Aya Takeoka, Ingmar Blümcke, Stephanie Möllmert, Kanwarpal Singh, Jochen Guck, Katja Kobow, Daniel Wehner
Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in humans and other mammals, whereas it facilitates axon regeneration in the zebrafish. However, the molecular basis of these different fates is not understood. Here, we identify small leucine-rich proteoglycans (SLRPs) as a contributing factor to regeneration failure in mammals. We demonstrate that the SLRPs chondroadherin, fibromodulin, lumican, and prolargin are enriched in rodent and human but not zebrafish CNS lesions...
October 26, 2023: Nature Communications
https://read.qxmd.com/read/37840262/involvement-of-small-leucine-rich-proteoglycans-and-telocytes-in-thin-and-thick-human-endometrium-immunohistochemical-and-ultrastructural-examination
#22
JOURNAL ARTICLE
Ozlem Karabay Akgul, Tugba Ekiz-Yilmaz
Thin endometrium, defined as an endometrial thickness of less than 7 mm during the late follicular phase, is a common cause of frequent cancelation of embryo transfers or recurrent implantation failure during assisted reproductive treatment. Small proteoglycans regulate intracellular signaling cascades by bridging other matrix molecules and tissue elements, affecting cell proliferation, adhesion, migration, and cytokine concentration. The aim of the study is to investigate the role of small leucine-rich proteoglycans in the pathogenesis of thin and thick human endometrium and their differences from normal endometrium in terms of fine structure properties...
October 16, 2023: Ultrastructural Pathology
https://read.qxmd.com/read/37834438/three-dimensional-modeling-of-cpg-dna-binding-with-matrix-lumican-shows-leucine-rich-repeat-motif-involvement-as-in-tlr9-cpg-dna-interactions
#23
JOURNAL ARTICLE
Tansol Choi, George Maiti, Shukti Chakravarti
Lumican is an extracellular matrix proteoglycan known to regulate toll-like receptor (TLR) signaling in innate immune cells. In experimental settings, lumican suppresses TLR9 signaling by binding to and sequestering its synthetic ligand, CpG-DNA, in non-signal permissive endosomes. However, the molecular details of lumican interactions with CpG-DNA are obscure. Here, the 3-D structure of the 22 base-long CpG-DNA (CpG ODN_2395) bound to lumican or TLR9 were modeled using homology modeling and docking methods...
October 8, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37793508/molecular-cues-for-immune-cells-from-small-leucine-rich-repeat-proteoglycans-in-their-extracellular-matrix-associated-and-free-forms
#24
REVIEW
George Maiti, Sean Ashworth, Tansol Choi, Shukti Chakravarti
In this review we highlight emerging immune regulatory functions of lumican, keratocan, fibromodulin, biglycan and decorin, which are members of the small leucine-rich proteoglycans (SLRP) of the extracellular matrix (ECM). These SLRPs have been studied extensively as collagen-fibril regulatory structural components of the skin, cornea, bone and cartilage in homeostasis. However, SLRPs released from a remodeling ECM, or synthesized by activated fibroblasts and immune cells contribute to an ECM-free pool in tissues and circulation, that may have a significant, but poorly understood foot print in inflammation and disease...
November 2023: Matrix Biology: Journal of the International Society for Matrix Biology
https://read.qxmd.com/read/37742739/rational-design-and-synthesis-of-lumican-stapled-peptides-for-promoting-corneal-wound-healing
#25
JOURNAL ARTICLE
Sudhir Verma, Fernando T Ogata, Isabel Y Moreno, Cassio Prinholato da Silva, Tainah Dorina Marforio, Matteo Calvaresi, Mehmet Sen, Vivien J Coulson-Thomas, Tarsis Ferreira Gesteira
PURPOSE: Lumican is a major extracellular matrix (ECM) component in the cornea that is upregulated after injury and promotes corneal wound healing. We have recently shown that peptides designed based on the 13 C-terminal amino acids of lumican (LumC13 and LumC13C-A ) are able to recapitulate the effects of lumican on promoting corneal wound healing. Herein we used computational chemistry to develop peptide mimetics derived from LumC13C-A with increased stability and half-life that are biologically active and non-toxic, thereby promoting corneal wound healing with increased pharmacological potential...
September 22, 2023: Ocular Surface
https://read.qxmd.com/read/37711155/lumican-silencing-ameliorates-%C3%AE-glycerophosphate-mediated-vascular-smooth-muscle-cell-calcification-by-attenuating-the-inhibition-of-apob-on-kif2c-activity
#26
JOURNAL ARTICLE
Haibin Li, Chunyan Zhang, Qiang Liu
Adverse cardiovascular events are associated with vascular calcification (VC) process, where vascular smooth muscle cells (VSMCs) differentiate into osteoblastic phenotype and deposit hydroxyapatite crystals. Microtubule-associated protein kinesin family member 2C (KIF2C) expression is decreased obviously in VSMC during calcification induction. Accordingly, we investigate the role and potential mechanism of KIF2C on VSMC calcification. The effects of β-glycerophosphate (β-GP)/KIF2C/lumican (LUM) on calcification, calcium content, alkaline phosphatase (ALP) activity, calcification-related markers, Tubulin, the ratio of polymerized (Po) to free (Fr) tubulin, as well as levels of LUM, apolipoprotein B (APOB), and KIF2C were assessed by Alizarin red S staining, calcium assay kit, ALP assay kit, Western blot, immunofluorescence, and quantitative real-time PCR...
2023: Open Medicine (Warsaw, Poland)
https://read.qxmd.com/read/37692065/multi-omics-analysis-reveals-the-prognostic-and-tumor-micro-environmental-value-of-lumican-in-multiple-cancer-types
#27
JOURNAL ARTICLE
Zehuai Guo, Zeyun Li, Ming Chen, Xiangjun Qi, Zhe Sun, Siqi Wu, Xuenan Hou, Mengli Qiu, Yang Cao
Background: Lumican (LUM), a proteoglycan of the extracellular matrix, has been reported to be involved in the regulation of immune escape processes, but the data supporting this phenomenon are not sufficient. In this study, we aimed to explore the links among LUM expression, survival, tumor microenvironment (TME), and immunotherapy in 33 cancer types. Methods: Data from several databases, such as UCSC Xena, GTEx, UALCAN, HPA, GEPIA2, TISIDB, PrognoScan, TIMER2, and GEO, as well as published studies, were used to determine the relationship between LUM expression and clinical features, TME, heterogeneity, and tumor stemness...
2023: Frontiers in Molecular Biosciences
https://read.qxmd.com/read/37662233/3d-modeling-of-cpg-dna-binding-with-matrix-lumican-shows-leucine-rich-repeat-motif-involvement-as-in-tlr9-cpg-dna-interactions
#28
Tansol Choi, George Maiti, Shukti Chakravarti
Lumican is an extracellular matrix proteoglycan, known to regulate toll-like receptor (TLR) signaling in innate immune cells. In experimental settings, lumican suppresses TLR9 signaling by binding to, and sequestering its synthetic ligand, CpG-DNA, in non-signal permissive endosomes. However, the molecular details of lumican interactions with CpG-DNA are obscure. Here, the 3-D structure of the 22 base-long CpG-DNA (CpG ODN_2395) bound to lumican or TLR9 were modeled using homology modeling and docking methods...
August 22, 2023: bioRxiv
https://read.qxmd.com/read/37580001/hyperprolactinemia-modifies-extracellular-matrix-components-associated-with-collagen-fibrillogenesis-in-harderian-glands-of-non-and-pregnant-female-mice
#29
JOURNAL ARTICLE
Ariadne S L Araujo, Manuel de J Simões, Osvaldo P Araujo-Jr, Ricardo S Simões, Edmund C Baracat, Helena B Nader, José M Soares-Jr, Regina C T Gomes
The harderian gland (HG) is a gland located at the base of the nictating membrane and fills the inferomedial aspect of the orbit in rodents. It is under the influence of the hypothalamic-pituitary-gonadal axis and, because of its hormone receptors, it is a target tissue for prolactin (PRL) and sex steroid hormones (estrogen and progesterone). In humans and murine, the anterior surface of the eyes is protected by a tear film synthesized by glands associated with the eye. In order to understand the endocrine changes caused by hyperprolactinemia in the glands responsible for the formation of the tear film, we used an animal model with metoclopramide-induced hyperprolactinemia (HPRL)...
August 12, 2023: Experimental Eye Research
https://read.qxmd.com/read/37509212/the-landscape-of-small-leucine-rich-proteoglycan-impact-on-cancer-pathogenesis-with-a-focus-on-biglycan-and-lumican
#30
REVIEW
Aikaterini Berdiaki, Eirini-Maria Giatagana, George Tzanakakis, Dragana Nikitovic
Cancer development is a multifactorial procedure that involves changes in the cell microenvironment and specific modulations in cell functions. A tumor microenvironment contains tumor cells, non-malignant cells, blood vessels, cells of the immune system, stromal cells, and the extracellular matrix (ECM). The small leucine-rich proteoglycans (SLRPs) are a family of nineteen proteoglycans, which are ubiquitously expressed among mammalian tissues and especially abundant in the ECM. SLRPs are divided into five canonical classes (classes I-III, containing fourteen members) and non-canonical classes (classes IV-V, including five members) based on their amino-acid structural sequence, chromosomal organization, and functional properties...
July 9, 2023: Cancers
https://read.qxmd.com/read/37483824/lumican-is-a-potential-predictor-on-the-efficacy-of-concurrent-chemoradiotherapy-in-cervical-squamous-cell-carcinoma
#31
JOURNAL ARTICLE
Ge Hu, Ying Xiao, Chanchan Ma, Jinyun Wang, Xiaotao Qian, Xiaowei Wu, Fengqin Zhu, Shiying Sun, Junchao Qian
PURPOSE: To identify new novel biomarkers for predicting the efficacy of concurrent chemoradiotherapy(CCRT) in cervical squamous cell carcinoma(CESC). METHODS: Gene expression datasets GSE56363, GSE5787, and GSE168009 were analyzed to identify candidate genes to predict the efficacy of CCRT in CESC. Single-cell RNA sequencing (scRNA-seq) data from GSE168652 and CESC patients in The Cancer Genome Atlas(TCGA) were systematically analyzed to explore possible molecular mechanisms...
July 2023: Heliyon
https://read.qxmd.com/read/37443910/exploration-of-mediators-associated-with-myocardial-remodelling-in-feline-hypertrophic-cardiomyopathy
#32
JOURNAL ARTICLE
Wan-Ching Cheng, Charlotte Lawson, Hui-Hsuan Liu, Lois Wilkie, Melanie Dobromylskyj, Virginia Luis Fuentes, Jayesh Dudhia, David J Connolly
Hypertrophic cardiomyopathy (HCM) affects both humans and cats and exhibits considerable interspecies similarities that are exemplified by underlying pathological processes and clinical presentation to the extent that developments in the human field may have direct relevance to the feline disease. Characteristic changes on histological examination include cardiomyocyte hypertrophy and interstitial and replacement fibrosis. Clinically, HCM is characterised by significant diastolic dysfunction due to a reduction in ventricular compliance and relaxation associated with extracellular matrix (ECM) remodelling and the development of ventricular hypertrophy...
June 26, 2023: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/37392962/the-endothelial-to-mesenchymal-transition-changes-the-focal-adhesion-site-proteins-levels-and-the-slrp-lumican-level-in-hmec-1%C3%A2-cell-line
#33
JOURNAL ARTICLE
Magdalena Wiktorska, Izabela Sacewicz-Hofman, Jolanta Niewiarowska
Scleroderma, the chronic autoimmune disease is a consequence of inflammation in the connective tissue. Prolonged duration affects formation of compact connective tissue strands (scarring) within the target organ. Endothelial cells undergoing endothelial-to-mesenchymal transition (EndMT) are the source of fibroblast phenotype-resembling cells. EndMT contributes to reorganization of the focal adhesion proteins (FA), including integrins, and intensive extracellular matrix (ECM) remodelling. However, in endothelial cells, the relationship between EndMT and the interaction of integrin receptors with lumican - a component of ECM, is still unclear...
June 29, 2023: Experimental Cell Research
https://read.qxmd.com/read/37382175/extracellular-matrix-related-and-serine-protease-proteins-in-the-amniotic-fluid-of-women-with-early-preterm-labor-association-with-spontaneous-preterm-birth-intra-amniotic-inflammation-and-microbial-invasion-of-the-amniotic-cavity
#34
JOURNAL ARTICLE
Kyong-No Lee, Kyo Hoon Park, Kwanghee Ahn, Eun Mi Im, Eunji Oh, Iseop Cho
PROBLEM: We aimed to determine whether altered levels of various extracellular matrix (ECM)-related and serine protease proteins in the amniotic fluid (AF) are associated with imminent spontaneous preterm birth (SPTB; ≤7 days) and intra-amniotic inflammation and/or microbial invasion of the amniotic cavity (IAI/MIAC) in women with early preterm labor (PTL). METHOD OF STUDY: This retrospective cohort study included 252 women with singleton pregnancies undergoing transabdominal amniocentesis who demonstrated PTL (24-31 weeks)...
July 2023: American Journal of Reproductive Immunology: AJRI
https://read.qxmd.com/read/37378898/the-novel-slrp-family-member-lumican-suppresses-pancreatic-cancer-cell-growth
#35
JOURNAL ARTICLE
Huijie Gao, Chao Liu, Qiang Ren, Litao Zhang, Wei Qin, Huiyun Wang, Yuntao Zhang
OBJECTIVES: The past studies clearly indicated that lumican was important in the context of pancreatic cancer (PC) onset and progression, but failed to clarify the underlying mechanistic basis for such activity. As such, we evaluated the functional importance of lumican in the context of pancreatic ductal adenocarcinoma (PDAC) to understand its mechanistic role in PC. METHODS: Lumican levels were evaluated in PDAC patient tissues via quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemistry approaches...
January 1, 2023: Pancreas
https://read.qxmd.com/read/37355022/small-leucine-rich-proteoglycans-biology-function-and-their-therapeutic-potential-in-the-ocular-surface
#36
JOURNAL ARTICLE
Tarsis Ferreira Gesteira, Sudhir Verma, Vivien J Coulson-Thomas
Small leucine rich proteoglycans (SLRPs) are the largest family of proteoglycans, with 18 members that are subdivided into five classes. SLRPs are small in size and can be present in tissues as glycosylated and non-glycosylated proteins, and the most studied SLRPs include decorin, biglycan, lumican, keratocan and fibromodulin. SLRPs specifically bind to collagen fibrils, regulating collagen fibrillogenesis and the biomechanical properties of tissues, and are expressed at particularly high levels in fibrous tissues, such as the cornea...
June 22, 2023: Ocular Surface
https://read.qxmd.com/read/37327869/the-versatile-roles-of-lumican-in-eye-diseases-a-review
#37
JOURNAL ARTICLE
Mei-Chi Tsui, Hsin-Yu Liu, Hsiao-Sang Chu, Wei-Li Chen, Fung-Rong Hu, Winston W-Y Kao, I-Jong Wang
Lumican is a keratan sulfate proteoglycan that belongs to the small leucine-rich proteoglycan family. Research has lifted the veil on the versatile roles of lumican in the pathogenesis of eye diseases. Lumican has pivotal roles in the maintenance of physiological tissue homogenesis and is often upregulated in pathological conditions, e.g., fibrosis, scar tissue formation in injured tissues, persistent inflammatory responses and immune anomaly, etc. Herein, we will review literature regarding the role of lumican in pathogenesis of inherited congenital and acquired eye diseases, e...
June 14, 2023: Ocular Surface
https://read.qxmd.com/read/37240735/application-of-lactobacillus-reuteri-b1-1-limosilactobacillus-reuteri-improves-immunological-profile-of-the-non-carcinogenic-porcine-derived-enterocytes
#38
JOURNAL ARTICLE
Viera Karaffová, Jana Teleky, Maša Pintarič, Tomaž Langerholc, Dagmar Mudroňová, Erik Hudec, Zuzana Ševčíková
In our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri ( L. reuteri ), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 × 107 and 1 × 109 CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1β, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB)...
April 27, 2023: Life
https://read.qxmd.com/read/37240176/derivation-of-human-corneal-keratocytes-from-relex-smile-lenticules-for-cell-therapy-and-tissue-engineering
#39
JOURNAL ARTICLE
Maria A Surovtseva, Irina I Kim, Natalia A Bondarenko, Alexander P Lykov, Kristina Yu Krasner, Elena V Chepeleva, Nataliya P Bgatova, Alexander N Trunov, Valery V Chernykh, Olga V Poveshchenko
Fibroblasts isolated and expanded from ReLEx SMILE lenticules can be a source of human keratocytes. Since corneal keratocytes are quiescent cells, it is difficult to expand them in vitro in suitable numbers for clinical and experimental use. In the present study, this problem was solved by isolating and growing corneal fibroblasts (CFs) with a high proliferative potential and their reversion to keratocytes in a selective serum-free medium. Fibroblasts reversed into keratocytes (rCFs) had a dendritic morphology and ultrastructural signs of activation of protein synthesis and metabolism...
May 16, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37034263/development-of-stromal-differentiation-patterns-in-heterotypical-models-of-artificial-corneas-generated-by-tissue-engineering
#40
JOURNAL ARTICLE
Cristina Blanco-Elices, Carmen Morales-Álvarez, Jesús Chato-Astrain, Carmen González-Gallardo, Paula Ávila-Fernández, Fernando Campos, Ramón Carmona, Miguel Ángel Martín-Piedra, Ingrid Garzón, Miguel Alaminos
Purpose: We carried out a histological characterization analysis of the stromal layer of human heterotypic cornea substitutes generated with extra-corneal cells to determine their putative usefulness in tissue engineering. Methods: Human bioartificial corneas were generated using nanostructured fibrin-agarose biomaterials with corneal stromal cells immersed within. To generate heterotypical corneas, umbilical cord Wharton's jelly stem cells (HWJSC) were cultured on the surface of the stromal substitutes to obtain an epithelial-like layer...
2023: Frontiers in Bioengineering and Biotechnology
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